CF833010

Overview
NameCF833010
Unique NameCF833010
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length849
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig9752 contig Csv1_Contig9752:263..1111. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF833010 vs. ExPASy Swiss-Prot
Match: METE_SOLSC (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Solenostemon scutellarioides GN=MET PE=1 SV=2)

HSP 1 Score: 370.933 bits (951), Expect = 4.908e-102
Identity = 185/191 (96.86%), Postives = 186/191 (97.38%), Query Frame = -1 
Query:  277 PRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQLASAK 849
            PR ETCYQIALAIKDEVEDLEKAGI VIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITN GVQDTTQIHTHMCYSNFNDIIHSII+MDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKY EVKPAL NMVAAAKLLRTQLASAK
Sbjct:  574 PRFETCYQIALAIKDEVEDLEKAGITVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNVGVQDTTQIHTHMCYSNFNDIIHSIINMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYAEVKPALENMVAAAKLLRTQLASAK 764
[Close]
BLAST of CF833010 vs. ExPASy Swiss-Prot
Match: METE_ARATH (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Arabidopsis thaliana GN=CIMS PE=1 SV=1)

HSP 1 Score: 368.237 bits (944), Expect = 3.181e-101
Identity = 180/191 (94.24%), Postives = 186/191 (97.38%), Query Frame = -1 
Query:  277 PRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQLASAK 849
            PRHETCYQIALAIKDEVEDLEK GI VIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQD+TQIHTHMCYS+FNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPS+EEIADR+NKMLAVLE NILWVNPDCGLKTRKYTEVKPAL NMV AAKL+R+QLASAK
Sbjct:  575 PRHETCYQIALAIKDEVEDLEKGGIGVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDSTQIHTHMCYSHFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSSEEIADRVNKMLAVLEQNILWVNPDCGLKTRKYTEVKPALKNMVDAAKLIRSQLASAK 765
[Close]
BLAST of CF833010 vs. ExPASy Swiss-Prot
Match: METE_CATRO (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Catharanthus roseus GN=METE PE=2 SV=1)

HSP 1 Score: 363.229 bits (931), Expect = 1.023e-99
Identity = 179/191 (93.72%), Postives = 185/191 (96.86%), Query Frame = -1 
Query:  277 PRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQLASAK 849
            PR ETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRK+EHAFYLDWAVHSFRITN  +QDTTQIHTHMCYSNFNDIIHSIIDMDADV+TIENSRS EKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVL+TNILWVNPDCGLKTRKY EVKPAL NMV+AAKL+RTQLASAK
Sbjct:  575 PRFETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKAEHAFYLDWAVHSFRITNLPLQDTTQIHTHMCYSNFNDIIHSIIDMDADVMTIENSRSSEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLDTNILWVNPDCGLKTRKYAEVKPALENMVSAAKLIRTQLASAK 765
[Close]
BLAST of CF833010 vs. ExPASy Swiss-Prot
Match: METE_MESCR (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Mesembryanthemum crystallinum GN=METE PE=2 SV=1)

HSP 1 Score: 357.836 bits (917), Expect = 4.300e-98
Identity = 177/191 (92.67%), Postives = 182/191 (95.29%), Query Frame = -1 
Query:  277 PRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQLASAK 849
            PRHETCYQIALAIK+EVEDLEKAGINVIQIDEAALREGLPLRKSEH FYL WAVHSFRITN GVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIP TEE+ADRI KMLAVLE+N+LWVNPDCGLKTRKY EV PALSNMVAAAK LR +LASAK
Sbjct:  575 PRHETCYQIALAIKNEVEDLEKAGINVIQIDEAALREGLPLRKSEHDFYLKWAVHSFRITNVGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPPTEELADRIRKMLAVLESNVLWVNPDCGLKTRKYGEVNPALSNMVAAAKQLRQELASAK 765
[Close]
BLAST of CF833010 vs. ExPASy Swiss-Prot
Match: METE_BURS3 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia sp. (strain 383) GN=metE PE=3 SV=1)

HSP 1 Score: 244.973 bits (624), Expect = 4.062e-64
Identity = 126/187 (67.38%), Postives = 143/187 (76.47%), Query Frame = -1 
Query:  289 PRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQL 849
            PR  +CYQ+ALAI++EV DLEKAG+ VIQIDEAALREGLPLR+++   YL WAV SFRIT  GVQD TQIHTHMCYS FNDII SI DMDADVITIE SRSD +LL  F +  KY   IGPGVYDIHSP IP+ E I   + K    +    LWVNPDCGLKTR++ EV PAL+NMVAAAK LR Q+
Sbjct:  578 PRSVSCYQLALAIREEVLDLEKAGVRVIQIDEAALREGLPLRRAQWGEYLKWAVESFRITANGVQDDTQIHTHMCYSEFNDIIASIADMDADVITIETSRSDMELLDAF-DNFKYPNEIGPGVYDIHSPNIPTQEHIVGLMKKAAERIPAERLWVNPDCGLKTRQWAEVIPALTNMVAAAKTLRNQV 763
[Close]
BLAST of CF833010 vs. ExPASy Swiss-Prot
Match: METE_BURCC (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia cenocepacia (strain MC0-3) GN=metE PE=3 SV=1)

HSP 1 Score: 244.202 bits (622), Expect = 6.928e-64
Identity = 125/187 (66.84%), Postives = 145/187 (77.54%), Query Frame = -1 
Query:  289 PRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQL 849
            PR  +CYQ+ALAI++EV DLEKAG+ VIQIDEAALREGLPLR+++ + YL WAV SFRIT  GVQD TQIHTHMCYS FNDII SI DMDADVITIE SRSD +LL  F +  KY   IGPGVYDIHSP IP+ + I   + K    +    LWVNPDCGLKTR++ EV PAL+NMVAAAK+LR Q+
Sbjct:  578 PRAVSCYQLALAIREEVLDLEKAGVRVIQIDEAALREGLPLRRAQWSEYLKWAVESFRITANGVQDDTQIHTHMCYSEFNDIIASIADMDADVITIETSRSDMELLDAF-DTFKYPNEIGPGVYDIHSPNIPTQDHIVGLMRKAAERIPAERLWVNPDCGLKTRQWAEVIPALTNMVAAAKMLRNQV 763
[Close]
BLAST of CF833010 vs. ExPASy Swiss-Prot
Match: METE_BURCM (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) GN=metE PE=3 SV=1)

HSP 1 Score: 243.047 bits (619), Expect = 1.543e-63
Identity = 124/187 (66.31%), Postives = 143/187 (76.47%), Query Frame = -1 
Query:  289 PRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQL 849
            PR  +CYQ+ALAI+DEV DLEKAG+ VIQIDEAALREGLPLR+++   YL WAV +FRIT  GVQD TQIHTHMCYS FNDII SI DMDADVITIE SRSD +LL  F +  +Y   IGPGVYDIHSP IP+ + I   + K    +    LWVNPDCGLKTR++ EV PAL+NMVAAAK LR Q+
Sbjct:  578 PRSVSCYQLALAIRDEVLDLEKAGVRVIQIDEAALREGLPLRRAQWGEYLKWAVEAFRITANGVQDDTQIHTHMCYSEFNDIIASIADMDADVITIETSRSDMELLDAF-DSFRYPNEIGPGVYDIHSPNIPTQDHIVGLMKKAAERIPAERLWVNPDCGLKTRQWAEVIPALTNMVAAAKTLRNQV 763
[Close]
BLAST of CF833010 vs. ExPASy Swiss-Prot
Match: METE_BURCJ (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia cepacia (strain J2315 / LMG 16656) GN=metE PE=3 SV=1)

HSP 1 Score: 243.047 bits (619), Expect = 1.543e-63
Identity = 125/187 (66.84%), Postives = 144/187 (77.01%), Query Frame = -1 
Query:  289 PRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQL 849
            PR  +CYQ+ALAI+DEV DLE+AG+ VIQIDEAALREGLPLR+++ + YL WAV SFRIT  GVQD TQIHTHMCYS FNDII SI DMDADVITIE SRSD +LL  F +  KY   IGPGVYDIHSP IP+ + I   + K    +    LWVNPDCGLKTR++ EV PAL+NMVAAAK LR Q+
Sbjct:  578 PRSVSCYQLALAIRDEVLDLEQAGVRVIQIDEAALREGLPLRRAQWSEYLKWAVESFRITANGVQDDTQIHTHMCYSEFNDIIASIADMDADVITIETSRSDMELLDAF-DDFKYPNEIGPGVYDIHSPNIPTQDHIVGLMRKAAERIPAERLWVNPDCGLKTRQWGEVIPALTNMVAAAKTLRNQV 763
[Close]
BLAST of CF833010 vs. ExPASy Swiss-Prot
Match: METE_BURCH (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia cenocepacia (strain HI2424) GN=metE PE=3 SV=1)

HSP 1 Score: 243.047 bits (619), Expect = 1.543e-63
Identity = 125/187 (66.84%), Postives = 144/187 (77.01%), Query Frame = -1 
Query:  289 PRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQL 849
            PR  +CYQ+ALAI++EV DLEKAG+ VIQIDEAALREGLPLR+++ + YL WAV SFRIT  GVQD TQIHTHMCYS FNDII SI DMDADVITIE SRSD +LL  F +  KY   IGPGVYDIHSP IP+ + I   + K    +    LWVNPDCGLKTR++ EV PAL+NMVAAAK LR Q+
Sbjct:  578 PRAVSCYQLALAIREEVLDLEKAGVRVIQIDEAALREGLPLRRAQWSEYLKWAVESFRITANGVQDDTQIHTHMCYSEFNDIIASIADMDADVITIETSRSDMELLDAF-DTFKYPNEIGPGVYDIHSPNIPTQDHIVGLMKKAAERIPAERLWVNPDCGLKTRQWAEVIPALTNMVAAAKTLRNQV 763
[Close]
BLAST of CF833010 vs. ExPASy Swiss-Prot
Match: METE_BURCA (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia cenocepacia (strain AU 1054) GN=metE PE=3 SV=1)

HSP 1 Score: 243.047 bits (619), Expect = 1.543e-63
Identity = 125/187 (66.84%), Postives = 144/187 (77.01%), Query Frame = -1 
Query:  289 PRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQL 849
            PR  +CYQ+ALAI++EV DLEKAG+ VIQIDEAALREGLPLR+++ + YL WAV SFRIT  GVQD TQIHTHMCYS FNDII SI DMDADVITIE SRSD +LL  F +  KY   IGPGVYDIHSP IP+ + I   + K    +    LWVNPDCGLKTR++ EV PAL+NMVAAAK LR Q+
Sbjct:  578 PRAVSCYQLALAIREEVLDLEKAGVRVIQIDEAALREGLPLRRAQWSEYLKWAVESFRITANGVQDDTQIHTHMCYSEFNDIIASIADMDADVITIETSRSDMELLDAF-DTFKYPNEIGPGVYDIHSPNIPTQDHIVGLMKKAAERIPAERLWVNPDCGLKTRQWAEVIPALTNMVAAAKTLRNQV 763
The following BLAST results are available for this feature:
BLAST of CF833010 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 283
Match NameE-valueIdentityDescription
METE_SOLSC4.908e-10296.865-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_ARATH3.181e-10194.245-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_CATRO1.023e-9993.725-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_MESCR4.300e-9892.675-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURS34.062e-6467.385-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURCC6.928e-6466.845-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURCM1.543e-6366.315-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURCJ1.543e-6366.845-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURCH1.543e-6366.845-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURCA1.543e-6366.845-methyltetrahydropteroyltriglutamate--homocystein... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_02N01_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa02N01, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF833010 ID=CF833010; Name=CF833010; organism=Citrus sinensis; type=EST; length=849bp
TTTTTTTTTTTTTTATGCACAAACCAGTAAAGGTATTCCATCGATTTACG
GCCACTGATAGCAAGCCGGGGCCTTTAAAAACGGAAAATACAAGCAAAGA
AAAATAGAAGCCAATTGCTCAATAGTGCATAATATGCATACGGAAAATTT
TCATCACATCACGACCCACAGTTATTCGAATAGGTAATAAGACAGGAATT
TTCTTTCTTTGTTTAAAGTGAAAAAAAAAAAAATCAATGGCTCAGGAAGT
ATCCCTCCAATCTCCAGAACCACTCACTTGGCACTGGCAAGCTGGGTGCG
GAGTAGCTTGGCAGCAGCGACCATGTTTGAGAGGGCTGGCTTCACCTCAG
TGTACTTGCGAGTCTTAAGACCACAGTCAGGGTTAACCCACAAAATGTTG
GTTTCTAGCACAGCAAGCATCTTGTTGATTCTGTCAGCAATTTCTTCAGT
TGATGGTATTCTTGGAGAGTGGATGTCATACACACCAGGGCCAATTCCAG
CACCATACTTGACTCCCTCACGGAAGACAGACAGGAGCTTCTCATCAGAA
CGAGAGTTCTCAATGGTGATCACATCAGCATCCATGTCGATAATTGAGTG
GATAATGTCGTTGAAGTTGGAGTAGCACATGTGGGTGTGGATCTGGGTAG
TGTCCTGGACGCCACAGTTGGTGATTCTGAAGGAGTGGACTGCCCAGTCC
AAGTAGAAAGCATGCTCAGATTTCCTAAGAGGCAATCCTTCCCTCAAGGC
AGCCTCATCAATTTGAATAACGTTAATGCCTGCCTTCTCAAGATCCTCGA
CTTCGTCCTTGATGGCCAAAGCAATCTGGTAGCAGGTTTCATGTCTGGG
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