CF834558

Overview
NameCF834558
Unique NameCF834558
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length522
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig460 contig Csv1_Contig460:211..732. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF834558 vs. ExPASy Swiss-Prot
Match: CYSK_CITLA (Cysteine synthase OS=Citrullus lanatus PE=2 SV=1)

HSP 1 Score: 222.631 bits (566), Expect = 8.652e-58
Identity = 117/150 (78.00%), Postives = 125/150 (83.33%), Query Frame = -1 
Query:   70 RIDALVSXXXXXXXXXXXGKYLKEHNPEIKLYGVEPVESAVLSGGKPGPHKIQGIGAGFIPGVLDVNLLDETVQISSEEAIETAKLLALKEGLLVGISSGXXXXXXXXXAKRPENAGKLIVVVFPSFGERYLSSVLFESVKKEAESMVFE 519
            +IDALVS           GKYLKE NP IKLYGVEPVESA+LSGGKPGPHKIQGIGAGFIPGVLDVNLLDE +Q+SSEE+IETAKLLALKEGLLVGISSG         AKRPENAGKLIV VFPSFGERYLS+VLFESVK+E E+MVFE
Sbjct:  175 KIDALVSGIGTGGTVTGAGKYLKEQNPNIKLYGVEPVESAILSGGKPGPHKIQGIGAGFIPGVLDVNLLDEVIQVSSEESIETAKLLALKEGLLVGISSGAAAAAAIRIAKRPENAGKLIVAVFPSFGERYLSTVLFESVKRETENMVFE 324
[Close]
BLAST of CF834558 vs. ExPASy Swiss-Prot
Match: CYSK_MAIZE (Cysteine synthase OS=Zea mays PE=1 SV=2)

HSP 1 Score: 215.698 bits (548), Expect = 1.058e-55
Identity = 113/150 (75.33%), Postives = 124/150 (82.67%), Query Frame = -1 
Query:   70 RIDALVSXXXXXXXXXXXGKYLKEHNPEIKLYGVEPVESAVLSGGKPGPHKIQGIGAGFIPGVLDVNLLDETVQISSEEAIETAKLLALKEGLLVGISSGXXXXXXXXXAKRPENAGKLIVVVFPSFGERYLSSVLFESVKKEAESMVFE 519
            +ID LVS           G+YL+E NP +KLYGVEPVESAVL+GGKPGPHKIQGIGAGFIPGVLDV+LLDET+Q+SS+EAIETAK LALKEGLLVGISSG         AKRPENAGKL VVVFPSFGERYLSSVLF+S+KKEAESMV E
Sbjct:  175 KIDGLVSGIGTGGTITGTGRYLREQNPNVKLYGVEPVESAVLNGGKPGPHKIQGIGAGFIPGVLDVDLLDETLQVSSDEAIETAKALALKEGLLVGISSGAAAAAAVRLAKRPENAGKLFVVVFPSFGERYLSSVLFQSIKKEAESMVVE 324
[Close]
BLAST of CF834558 vs. ExPASy Swiss-Prot
Match: CYSK_SPIOL (Cysteine synthase OS=Spinacia oleracea PE=1 SV=1)

HSP 1 Score: 212.231 bits (539), Expect = 1.169e-54
Identity = 112/150 (74.67%), Postives = 122/150 (81.33%), Query Frame = -1 
Query:   70 RIDALVSXXXXXXXXXXXGKYLKEHNPEIKLYGVEPVESAVLSGGKPGPHKIQGIGAGFIPGVLDVNLLDETVQISSEEAIETAKLLALKEGLLVGISSGXXXXXXXXXAKRPENAGKLIVVVFPSFGERYLSSVLFESVKKEAESMVFE 519
            +ID  VS           GKYLKE NP++KL G+EPVESAVLSGGKPGPHKIQG+GAGFIPGVLDVN++DE VQISSEE+IE AKLLALKEGLLVGISSG         AKRPENAGKLIV VFPSFGERYLSSVLF+SV+KEAESMV E
Sbjct:  175 KIDIFVSGIGTGGTITGAGKYLKEQNPDVKLIGLEPVESAVLSGGKPGPHKIQGLGAGFIPGVLDVNIIDEVVQISSEESIEMAKLLALKEGLLVGISSGAAAAAAIKVAKRPENAGKLIVAVFPSFGERYLSSVLFDSVRKEAESMVIE 324
[Close]
BLAST of CF834558 vs. ExPASy Swiss-Prot
Match: CYSK2_ORYSJ (Cysteine synthase OS=Oryza sativa subsp. japonica GN=RCS3 PE=2 SV=1)

HSP 1 Score: 212.231 bits (539), Expect = 1.169e-54
Identity = 109/150 (72.67%), Postives = 122/150 (81.33%), Query Frame = -1 
Query:   70 RIDALVSXXXXXXXXXXXGKYLKEHNPEIKLYGVEPVESAVLSGGKPGPHKIQGIGAGFIPGVLDVNLLDETVQISSEEAIETAKLLALKEGLLVGISSGXXXXXXXXXAKRPENAGKLIVVVFPSFGERYLSSVLFESVKKEAESMVFE 519
            ++D LVS           GKYLKE NPEIK+YGVEP ESA+LSGG+PGPHKIQGIGAGF+PGVLDVNLLDE VQ+SS+EAI  AK LALKEGLLVGISSG         A+RPEN GKL+VVVFPSFGERYLSSVLFES+K+EAE+MVFE
Sbjct:  175 KVDILVSGIGTGGTVTGTGKYLKEQNPEIKIYGVEPTESAILSGGRPGPHKIQGIGAGFVPGVLDVNLLDEVVQVSSDEAISMAKQLALKEGLLVGISSGAAAVAAIRVAQRPENKGKLVVVVFPSFGERYLSSVLFESIKREAENMVFE 324
[Close]
BLAST of CF834558 vs. ExPASy Swiss-Prot
Match: CYSK_SOLTU (Cysteine synthase OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 211.075 bits (536), Expect = 2.605e-54
Identity = 108/150 (72.00%), Postives = 123/150 (82.00%), Query Frame = -1 
Query:   70 RIDALVSXXXXXXXXXXXGKYLKEHNPEIKLYGVEPVESAVLSGGKPGPHKIQGIGAGFIPGVLDVNLLDETVQISSEEAIETAKLLALKEGLLVGISSGXXXXXXXXXAKRPENAGKLIVVVFPSFGERYLSSVLFESVKKEAESMVFE 519
            ++DAL S           GKYL+E NP +KLYGVEPVESA+LSGGKPGPHKIQGIGAGFIPGVL+VNL+D+ VQ+SSEE+IE AKLLALKEGLLVGISSG         AKRPENAGKLIVV+FPSFGERYLSSVLFE+V++EAE+M  E
Sbjct:  175 KVDALASGIGTGGTITGSGKYLREQNPNVKLYGVEPVESAILSGGKPGPHKIQGIGAGFIPGVLEVNLIDDVVQVSSEESIEMAKLLALKEGLLVGISSGAAAAAAIKVAKRPENAGKLIVVIFPSFGERYLSSVLFETVRREAENMTVE 324
[Close]
BLAST of CF834558 vs. ExPASy Swiss-Prot
Match: CYSK_WHEAT (Cysteine synthase OS=Triticum aestivum GN=CYS1 PE=2 SV=1)

HSP 1 Score: 207.994 bits (528), Expect = 2.205e-53
Identity = 108/150 (72.00%), Postives = 121/150 (80.67%), Query Frame = -1 
Query:   70 RIDALVSXXXXXXXXXXXGKYLKEHNPEIKLYGVEPVESAVLSGGKPGPHKIQGIGAGFIPGVLDVNLLDETVQISSEEAIETAKLLALKEGLLVGISSGXXXXXXXXXAKRPENAGKLIVVVFPSFGERYLSSVLFESVKKEAESMVFE 519
            +ID LVS           GKYL+E NP IKLYGVEP ESA+L+GGKPGPHKIQGIGAGFIPGVLDV+++DET+Q+SS+E+IE AK LALKEGLLVGISSG         A+RPENAGKL VVVFPSFGERYLSSVLF S+KKEAESMV E
Sbjct:  176 KIDGLVSGIGTGGTITGTGKYLQEQNPNIKLYGVEPTESAILNGGKPGPHKIQGIGAGFIPGVLDVDIIDETIQVSSDESIEMAKSLALKEGLLVGISSGAAAAAAIKVAQRPENAGKLFVVVFPSFGERYLSSVLFHSIKKEAESMVVE 325
[Close]
BLAST of CF834558 vs. ExPASy Swiss-Prot
Match: CYSK1_ORYSJ (Cysteine synthase OS=Oryza sativa subsp. japonica GN=RCS1 PE=2 SV=2)

HSP 1 Score: 207.994 bits (528), Expect = 2.205e-53
Identity = 107/150 (71.33%), Postives = 123/150 (82.00%), Query Frame = -1 
Query:   70 RIDALVSXXXXXXXXXXXGKYLKEHNPEIKLYGVEPVESAVLSGGKPGPHKIQGIGAGFIPGVLDVNLLDETVQISSEEAIETAKLLALKEGLLVGISSGXXXXXXXXXAKRPENAGKLIVVVFPSFGERYLSSVLFESVKKEAESMVFE 519
            ++D LVS           G+YL+E NP+IK+YGVEPVESAVLSGGKPGPHKIQGIGAGF+PGVLDV+L++ETVQ+SS+EAIE AK LALKEGLLVGISSG         A+RPEN GKL VVVFPSFGERYLSSVLF+S+KKEAE+MV E
Sbjct:  172 KVDGLVSGIGTGGTITGAGRYLREQNPDIKIYGVEPVESAVLSGGKPGPHKIQGIGAGFVPGVLDVDLINETVQVSSDEAIEMAKALALKEGLLVGISSGAAAAAAVRLAQRPENEGKLFVVVFPSFGERYLSSVLFQSIKKEAENMVVE 321
[Close]
BLAST of CF834558 vs. ExPASy Swiss-Prot
Match: CYSK1_BRAJU (Cysteine synthase OS=Brassica juncea PE=2 SV=1)

HSP 1 Score: 201.445 bits (511), Expect = 2.064e-51
Identity = 102/150 (68.00%), Postives = 119/150 (79.33%), Query Frame = -1 
Query:   70 RIDALVSXXXXXXXXXXXGKYLKEHNPEIKLYGVEPVESAVLSGGKPGPHKIQGIGAGFIPGVLDVNLLDETVQISSEEAIETAKLLALKEGLLVGISSGXXXXXXXXXAKRPENAGKLIVVVFPSFGERYLSSVLFESVKKEAESMVFE 519
            +ID  VS           GKYLKE NP +KLYGVEP+ESA+LSGGKPGPHKIQGIGAGFIP VL+V+L+DE VQ+SS+E+I+ A+LLALKEGLLVGISSG         AKRPENAGKL V VFPSFGERYLS+VLF++ +KEAESM F+
Sbjct:  172 KIDGFVSGIGTGGTITGAGKYLKEQNPNVKLYGVEPIESAILSGGKPGPHKIQGIGAGFIPSVLEVDLIDEVVQVSSDESIDMARLLALKEGLLVGISSGAAAAAAIKLAKRPENAGKLFVAVFPSFGERYLSTVLFDATRKEAESMTFQ 321
[Close]
BLAST of CF834558 vs. ExPASy Swiss-Prot
Match: CYSK2_BRAJU (Cysteine synthase OS=Brassica juncea PE=2 SV=1)

HSP 1 Score: 199.134 bits (505), Expect = 1.024e-50
Identity = 101/150 (67.33%), Postives = 117/150 (78.00%), Query Frame = -1 
Query:   70 RIDALVSXXXXXXXXXXXGKYLKEHNPEIKLYGVEPVESAVLSGGKPGPHKIQGIGAGFIPGVLDVNLLDETVQISSEEAIETAKLLALKEGLLVGISSGXXXXXXXXXAKRPENAGKLIVVVFPSFGERYLSSVLFESVKKEAESMVFE 519
            +ID  +S           GKYLKE NP +KLYGVEPVESA+LSGGKPGPHKIQGIGAGFIP VL+ NL+DE VQ+SS+E+I+ A+LLA +EGLLVGISSG         AKRPENAGKL V VFPSFGERYLS+VLF++ +KEAESM FE
Sbjct:  174 KIDGFISGIGTGGTITGAGKYLKEQNPNVKLYGVEPVESAILSGGKPGPHKIQGIGAGFIPNVLETNLIDEVVQVSSDESIDMARLLAREEGLLVGISSGAAAAAAIKLAKRPENAGKLFVAVFPSFGERYLSTVLFDATRKEAESMTFE 323
[Close]
BLAST of CF834558 vs. ExPASy Swiss-Prot
Match: CYSK1_ARATH (Cysteine synthase OS=Arabidopsis thaliana GN=OASA1 PE=1 SV=2)

HSP 1 Score: 194.126 bits (492), Expect = 3.295e-49
Identity = 99/150 (66.00%), Postives = 117/150 (78.00%), Query Frame = -1 
Query:   70 RIDALVSXXXXXXXXXXXGKYLKEHNPEIKLYGVEPVESAVLSGGKPGPHKIQGIGAGFIPGVLDVNLLDETVQISSEEAIETAKLLALKEGLLVGISSGXXXXXXXXXAKRPENAGKLIVVVFPSFGERYLSSVLFESVKKEAESMVFE 519
            +ID  VS           GKYLKE N  +KLYGVEPVESA+LSGGKPGPHKIQGIGAGFIP VL+V+L+DE VQ+SS+E+I+ A+ LALKEGLLVGISSG         A+RPENAGKL V +FPSFGERYLS+VLF++ +KEAE+M FE
Sbjct:  172 KIDGFVSGIGTGGTITGAGKYLKEQNANVKLYGVEPVESAILSGGKPGPHKIQGIGAGFIPSVLNVDLIDEVVQVSSDESIDMARQLALKEGLLVGISSGAAAAAAIKLAQRPENAGKLFVAIFPSFGERYLSTVLFDATRKEAEAMTFE 321
The following BLAST results are available for this feature:
BLAST of CF834558 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 47
Match NameE-valueIdentityDescription
CYSK_CITLA8.652e-5878.00Cysteine synthase OS=Citrullus lanatus PE=2 SV=1[more]
CYSK_MAIZE1.058e-5575.33Cysteine synthase OS=Zea mays PE=1 SV=2[more]
CYSK_SPIOL1.169e-5474.67Cysteine synthase OS=Spinacia oleracea PE=1 SV=1[more]
CYSK2_ORYSJ1.169e-5472.67Cysteine synthase OS=Oryza sativa subsp. japonica ... [more]
CYSK_SOLTU2.605e-5472.00Cysteine synthase OS=Solanum tuberosum PE=2 SV=1[more]
CYSK_WHEAT2.205e-5372.00Cysteine synthase OS=Triticum aestivum GN=CYS1 PE=... [more]
CYSK1_ORYSJ2.205e-5371.33Cysteine synthase OS=Oryza sativa subsp. japonica ... [more]
CYSK1_BRAJU2.064e-5168.00Cysteine synthase OS=Brassica juncea PE=2 SV=1[more]
CYSK2_BRAJU1.024e-5067.33Cysteine synthase OS=Brassica juncea PE=2 SV=1[more]
CYSK1_ARATH3.295e-4966.00Cysteine synthase OS=Arabidopsis thaliana GN=OASA1... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_05L20_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa05L20, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF834558 ID=CF834558; Name=CF834558; organism=Citrus sinensis; type=EST; length=522bp
CTACATCACCTCCCCATTACTCAAAAAATTTTGGATCGCTCACTCTCTCT
GTCTGGCTCTATCTCACTGTTCAAACACCATGCTCTCTGCCTCCTTTTTC
ACAGATTCAAATAAAACAGAAGAGAGGTATCGCTCTCCGAAGCTGGGGAA
GACCACCACAATGAGTTTTCCAGCATTTTCTGGCCTCTTTGCTATCCGAA
TTGCAGCTGCAGTAGCAGCTCCGGATGATATCCCAACCAACAATCCTTCT
TTAAGTGCAAGAAGCTTCGCAGTCTCAATAGCTTCTTCACTTGATATTTG
AACAGTTTCATCTAGTAAATTGACGTCCAAAACTCCAGGGATGAAGCCAG
CACCTATTCCTTGGATCTTATGGGGACCAGGTTTCCCTCCAGACAGCACA
GCACTTTCAACTGGTTCAACGCCATAGAGCTTAATTTCAGGATTATGCTC
TTTGAGATACTTCCCTGCACCAGTGACAGTACCACCAGTCCCTATACCAG
AAACAAGGGCATCAATTCTTAA
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