CF835832

Overview
NameCF835832
Unique NameCF835832
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length645
Libraries
Library NameType
Washington Navel Orange Shoot Meristem cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
CF835832_ssr469 microsatellite CF835832_ssr469:469..483. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig1088 contig Csv1_Contig1088:465..1109. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
BLAST of CF835832 vs. ExPASy Swiss-Prot
Match: RL27_PEA (60S ribosomal protein L27 OS=Pisum sativum GN=RPL27 PE=2 SV=1)

HSP 1 Score: 244.202 bits (622), Expect = 4.303e-64
Identity = 121/135 (89.63%), Postives = 126/135 (93.33%), Query Frame = 3
Query:   30 MVKFLKPNMAVILLQGRYAGRKAVIVKSFDDGTRERAYGHCLVAGIKKYPGKVIRKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKDVVSADSLQSKDKKVTACKETKKRLEERFKTGKNRWFFTKLRF 434
            MVKFLKPN AVILLQGRYAG+KAVIVK+FDDGTRE+ YGHCLVAGIKK+P KVI+KDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKD V  D LQSKDKKVTA KETKK LEERFKTGKNRWFFTKLRF
Sbjct:    1 MVKFLKPNKAVILLQGRYAGKKAVIVKTFDDGTREKPYGHCLVAGIKKFPSKVIKKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKDAVVPDVLQSKDKKVTALKETKKSLEERFKTGKNRWFFTKLRF 135          
BLAST of CF835832 vs. ExPASy Swiss-Prot
Match: RL273_ARATH (60S ribosomal protein L27-3 OS=Arabidopsis thaliana GN=RPL27C PE=2 SV=2)

HSP 1 Score: 235.728 bits (600), Expect = 1.531e-61
Identity = 114/135 (84.44%), Postives = 123/135 (91.11%), Query Frame = 3
Query:   30 MVKFLKPNMAVILLQGRYAGRKAVIVKSFDDGTRERAYGHCLVAGIKKYPGKVIRKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKDVVSADSLQSKDKKVTACKETKKRLEERFKTGKNRWFFTKLRF 434
            MVKFLK N AVILLQGRYAG+KAVI+KSFDDG R+R YGHCLVAG+KKYP KVIRKDSAKKTAKKSRVK F+KLVNYQHLMPTRYTLDVDLK+V + D+LQSKDKKV A KE K +LEERFKTGKNRWFFTKLRF
Sbjct:    1 MVKFLKQNKAVILLQGRYAGKKAVIIKSFDDGNRDRPYGHCLVAGLKKYPSKVIRKDSAKKTAKKSRVKCFIKLVNYQHLMPTRYTLDVDLKEVATLDALQSKDKKVAALKEAKAKLEERFKTGKNRWFFTKLRF 135          
BLAST of CF835832 vs. ExPASy Swiss-Prot
Match: RL272_ARATH (60S ribosomal protein L27-2 OS=Arabidopsis thaliana GN=RPL27B PE=2 SV=2)

HSP 1 Score: 235.728 bits (600), Expect = 1.531e-61
Identity = 114/135 (84.44%), Postives = 124/135 (91.85%), Query Frame = 3
Query:   30 MVKFLKPNMAVILLQGRYAGRKAVIVKSFDDGTRERAYGHCLVAGIKKYPGKVIRKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKDVVSADSLQSKDKKVTACKETKKRLEERFKTGKNRWFFTKLRF 434
            MVKFLK N AVILLQGRYAG+KAVI+KSFDDGT +R YGHCLVAG+KKYP KVIRKDSAKKTAKKSRVK F+KLVNYQHLMPTRYTLDVDLK+V + D+L+SKDKKVTA KE K +LEERFKTGKNRWFFTKLRF
Sbjct:    1 MVKFLKQNKAVILLQGRYAGKKAVIIKSFDDGTSDRRYGHCLVAGLKKYPSKVIRKDSAKKTAKKSRVKCFIKLVNYQHLMPTRYTLDVDLKEVATLDALKSKDKKVTALKEAKAKLEERFKTGKNRWFFTKLRF 135          
BLAST of CF835832 vs. ExPASy Swiss-Prot
Match: RL271_ARATH (60S ribosomal protein L27-1 OS=Arabidopsis thaliana GN=RPL27A PE=2 SV=1)

HSP 1 Score: 226.868 bits (577), Expect = 7.110e-59
Identity = 109/135 (80.74%), Postives = 121/135 (89.63%), Query Frame = 3
Query:   30 MVKFLKPNMAVILLQGRYAGRKAVIVKSFDDGTRERAYGHCLVAGIKKYPGKVIRKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKDVVSADSLQSKDKKVTACKETKKRLEERFKTGKNRWFFTKLRF 434
            MVK +KP  AVILLQGRY G+KAVIVKSFDDGT E+ YGHCLVAG+KKYP KVIRKDSAKKTAKKSRVK F K++NYQH+MPTRYTLD+DLK+VVSAD++ SKDKKVTA KE K + EERFKTGKNRWFFTKLRF
Sbjct:    1 MVKCMKPGKAVILLQGRYTGKKAVIVKSFDDGTVEKKYGHCLVAGLKKYPSKVIRKDSAKKTAKKSRVKCFFKVINYQHVMPTRYTLDLDLKNVVSADAISSKDKKVTALKEAKAKFEERFKTGKNRWFFTKLRF 135          
BLAST of CF835832 vs. ExPASy Swiss-Prot
Match: RL27_SOLTU (60S ribosomal protein L27 OS=Solanum tuberosum GN=RPL27 PE=2 SV=1)

HSP 1 Score: 216.083 bits (549), Expect = 1.255e-55
Identity = 109/139 (78.42%), Postives = 121/139 (87.05%), Query Frame = 3
Query:   30 MVKFLKPNMAVILLQGRYAGRKAVIVKSFDDGTRERAYGHCLVAGIKKYPGKVIRKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDL----KDVVSADSLQSKDKKVTACKETKKRLEERFKTGKNRWFFTKLRF 434
            M KFLKPN AVILLQG+YAGRKAVIV++FD+GTR+R YGHCLVAGI +YP KVIRKDSAKK  KKSRVKAF+KLVNY H+MPTRYTLD DL    KDVV+AD LQ++DKKVTA + TK RL ERFKTGKNRWFFTKLRF
Sbjct:    1 MEKFLKPNKAVILLQGKYAGRKAVIVRAFDEGTRDRPYGHCLVAGISRYPKKVIRKDSAKKAGKKSRVKAFIKLVNYNHIMPTRYTLDEDLKDVVKDVVNADVLQARDKKVTA-RRTKARLAERFKTGKNRWFFTKLRF 138          
BLAST of CF835832 vs. ExPASy Swiss-Prot
Match: RL27_PYRST (60S ribosomal protein L27 OS=Pyrobotrys stellata GN=RPL27 PE=2 SV=1)

HSP 1 Score: 176.407 bits (446), Expect = 1.102e-43
Identity = 87/135 (64.44%), Postives = 105/135 (77.78%), Query Frame = 3
Query:   30 MVKFLKPNMAVILLQGRYAGRKAVIVKSFDDGTRERAYGHCLVAGIKKYPGKVIRKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKDVVSADSLQSKDKKVTACKETKKRLEERFKTGKNRWFFTKLRF 434
            MVKFLK    V++L GR+AG+KAVIV++FDDGT  R YGH LV G++K P KV ++ S KK AKKS +K F+K VNY HLMPTRYTLDVD K  V+A++ ++  KKV A KE KK LEE+FKTGKNRWFFTKLRF
Sbjct:    1 MVKFLKSGKVVVVLSGRFAGKKAVIVRNFDDGTSSRPYGHALVVGLQKEPRKVTKRQSQKKQAKKSTLKTFIKTVNYNHLMPTRYTLDVDFKG-VAAEAQENPTKKVEARKECKKLLEEKFKTGKNRWFFTKLRF 134          
BLAST of CF835832 vs. ExPASy Swiss-Prot
Match: RL27_HIPCM (60S ribosomal protein L27 OS=Hippocampus comes GN=rpl27 PE=2 SV=2)

HSP 1 Score: 158.688 bits (400), Expect = 2.378e-38
Identity = 79/136 (58.09%), Postives = 98/136 (72.06%), Query Frame = 3
Query:   30 MVKFLKPNMAVILLQGRYAGRKAVIVKSFDDGTRERAYGHCLVAGIKKYPGKVIRKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDL-KDVVSADSLQSKDKKVTACKETKKRLEERFKTGKNRWFFTKLRF 434
            M KF+KP   V++L GRYAGRKAVIVK+ DDGT +R Y H LVAGI +YP KV      KK AK+S++KAFVK+ NY HLMPTRY++D+ L K VV+ D  +    K  A +E K + EER+KTGKN+WFF KLRF
Sbjct:    1 MGKFMKPGKVVMVLAGRYAGRKAVIVKNIDDGTADRPYSHALVAGIDRYPRKVTAPMGKKKIAKRSKIKAFVKVYNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKSKARREAKVKFEERYKTGKNKWFFQKLRF 136          
BLAST of CF835832 vs. ExPASy Swiss-Prot
Match: RL27_DANRE (60S ribosomal protein L27 OS=Danio rerio GN=rpl27 PE=2 SV=3)

HSP 1 Score: 158.303 bits (399), Expect = 3.106e-38
Identity = 79/136 (58.09%), Postives = 98/136 (72.06%), Query Frame = 3
Query:   30 MVKFLKPNMAVILLQGRYAGRKAVIVKSFDDGTRERAYGHCLVAGIKKYPGKVIRKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDL-KDVVSADSLQSKDKKVTACKETKKRLEERFKTGKNRWFFTKLRF 434
            M KF+KP   V++L GRYAGRKAVIVK+ DDGT +R Y H LVAGI +YP KV      KK AK+S++KAFVK+ NY HLMPTRY++D+ L K VV+ D  +    K  A +E K + EER+KTGKN+WFF KLRF
Sbjct:    1 MGKFMKPGKVVMVLAGRYAGRKAVIVKNIDDGTADRPYSHALVAGIDRYPRKVTATMGKKKIAKRSKIKAFVKVFNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF 136          
BLAST of CF835832 vs. ExPASy Swiss-Prot
Match: RL27_ICTPU (60S ribosomal protein L27 OS=Ictalurus punctatus GN=rpl27 PE=2 SV=3)

HSP 1 Score: 157.147 bits (396), Expect = 6.918e-38
Identity = 77/136 (56.62%), Postives = 98/136 (72.06%), Query Frame = 3
Query:   30 MVKFLKPNMAVILLQGRYAGRKAVIVKSFDDGTRERAYGHCLVAGIKKYPGKVIRKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDL-KDVVSADSLQSKDKKVTACKETKKRLEERFKTGKNRWFFTKLRF 434
            M KF+KP   V++L GRYAGRKAVIVK+ DDGT +R Y H LV+GI +YP KV      KK AK+S++KAFVK+ NY HLMPTRY++D+ L K +V+ D  +    K  A +E K + EER+KTGKN+WFF KLRF
Sbjct:    1 MGKFMKPGKVVMVLAGRYAGRKAVIVKNIDDGTADRPYSHALVSGIDRYPRKVTATMGKKKVAKRSKIKAFVKVFNYNHLMPTRYSVDIPLDKTIVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF 136          
BLAST of CF835832 vs. ExPASy Swiss-Prot
Match: RL27_RAT (60S ribosomal protein L27 OS=Rattus norvegicus GN=Rpl27 PE=2 SV=2)

HSP 1 Score: 156.377 bits (394), Expect = 1.180e-37
Identity = 77/136 (56.62%), Postives = 98/136 (72.06%), Query Frame = 3
Query:   30 MVKFLKPNMAVILLQGRYAGRKAVIVKSFDDGTRERAYGHCLVAGIKKYPGKVIRKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDL-KDVVSADSLQSKDKKVTACKETKKRLEERFKTGKNRWFFTKLRF 434
            M KF+KP   V++L GRY+GRKAVIVK+ DDGT +R Y H LVAGI +YP KV      KK AK+S++K+FVK+ NY HLMPTRY++D+ L K VV+ D  +    K  A +E K + EER+KTGKN+WFF KLRF
Sbjct:    1 MGKFMKPGKVVLVLAGRYSGRKAVIVKNIDDGTSDRPYSHALVAGIDRYPRKVTAAMGKKKIAKRSKIKSFVKVYNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF 136          
The following BLAST results are available for this feature:
BLAST of CF835832 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 25
Match NameE-valueIdentityDescription
RL27_PEA4.303e-6489.6360S ribosomal protein L27 OS=Pisum sativum GN=RPL2... [more]
RL273_ARATH1.531e-6184.4460S ribosomal protein L27-3 OS=Arabidopsis thalian... [more]
RL272_ARATH1.531e-6184.4460S ribosomal protein L27-2 OS=Arabidopsis thalian... [more]
RL271_ARATH7.110e-5980.7460S ribosomal protein L27-1 OS=Arabidopsis thalian... [more]
RL27_SOLTU1.255e-5578.4260S ribosomal protein L27 OS=Solanum tuberosum GN=... [more]
RL27_PYRST1.102e-4364.4460S ribosomal protein L27 OS=Pyrobotrys stellata G... [more]
RL27_HIPCM2.378e-3858.0960S ribosomal protein L27 OS=Hippocampus comes GN=... [more]
RL27_DANRE3.106e-3858.0960S ribosomal protein L27 OS=Danio rerio GN=rpl27 ... [more]
RL27_ICTPU6.918e-3856.6260S ribosomal protein L27 OS=Ictalurus punctatus G... [more]
RL27_RAT1.180e-3756.6260S ribosomal protein L27 OS=Rattus norvegicus GN=... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS03_01P12_r Washington Navel Orange Shoot Meristem cDNA Library Citrus sinensis cDNA clone CS_PEa01P12, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF835832 ID=CF835832; Name=CF835832; organism=Citrus sinensis; type=EST; length=645bp
CCGCGGGTGCGCCATGAGCGAGAGTTGAAATGGTGAAGTTTCTGAAACCA
AACATGGCCGTGATCCTTCTTCAGGGCCGTTACGCCGGCCGGAAGGCGGT
GATCGTGAAGTCGTTCGACGACGGGACCCGCGAGAGGGCGTACGGGCACT
GTTTGGTAGCGGGGATAAAGAAGTACCCGGGCAAGGTCATCCGCAAGGAC
TCGGCGAAGAAGACAGCGAAGAAGTCGCGCGTCAAGGCCTTCGTCAAGTT
GGTCAACTACCAGCACCTGATGCCCACGCGCTACACTCTCGACGTCGATT
TGAAGGACGTCGTGTCGGCCGATTCCCTCCAGAGCAAGGACAAGAAGGTG
ACCGCTTGCAAGGAGACCAAGAAGCGCCTCGAGGAGAGGTTTAAGACTGG
CAAGAATCGTTGGTTCTTTACTAAGCTCAGGTTTTAGATTTTTAACCAAA
GGAAGAAAGCAACTTGTCGTTTTGTTTTGTTTTGTGTTATTGAAAACGCT
TTAGTTGTTAAAGTGCGAATTTTGAGATTTATGTAGTGTTTTGTGAACTT
AATTTTTTCTGGATCTGGTACTATTTTTATGTGATATTATTGGGTTTATA
ATGTTTACTCAAGACGTCATAAATTNNANAAAAAAAAAAAAAAAA
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