CF931512

Overview
NameCF931512
Unique NameCF931512
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length715
Libraries
Library NameType
Mature peel (flavedo) cDNA subtraction librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
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BLAST of CF931512 vs. ExPASy Swiss-Prot
Match: ROGF1_ARATH (Rop guanine nucleotide exchange factor 1 OS=Arabidopsis thaliana GN=ROPGEF1 PE=1 SV=2)

HSP 1 Score: 116.701 bits (291), Expect = 1.239e-25
Identity = 60/137 (43.80%), Postives = 94/137 (68.61%), Query Frame = 3 
Query:    9 LTAESSSSGEMINFLNLKSEHSALEAINKLEAAVFTWKERISEQASGKSPVR-TSWSF-IKDPISELDKIEFLLERAEALIQQLKNRYPNLPQTFLDATKIQYGKDVGHSILEAYSRVLANLAFSILSRIGDILQED 413
            LTA   S   +++ L+L SEH  LE  N++EAAV  W+++   +   ++ ++ +SW   +K  +++ ++ +FL++RAE L+Q L+ R+P LPQT LD  KIQY KDVG SILE+YSRV+ ++AF+I +RI D+L  D
Sbjct:  316 LTANKFSPECLLDCLDLSSEHQTLEIANRIEAAVHVWRQKNGRRHKKQAKLKLSSWGGKVKGLVNDNERNDFLVQRAETLLQSLRIRFPGLPQTTLDMNKIQYNKDVGQSILESYSRVMESMAFNITARIDDVLYVD 452
[Close]
BLAST of CF931512 vs. ExPASy Swiss-Prot
Match: ROGF2_ARATH (Rop guanine nucleotide exchange factor 2 OS=Arabidopsis thaliana GN=ROPGEF2 PE=1 SV=2)

HSP 1 Score: 99.7525 bits (247), Expect = 1.568e-20
Identity = 56/132 (42.42%), Postives = 84/132 (63.64%), Query Frame = 3 
Query:   27 SSGEMINFLNLKSEHSALEAINKLEAAVFTWKERISEQASGKSPVRTSWSFIKDPIS---ELDKIEFLLERAEALIQQLKNRYPNLPQTFLDATKIQYGKDVGHSILEAYSRVLANLAFSILSRIGDILQED 413
            S  ++++ L + SEH AL+  +++EA+++TW+ +     S     ++SW+ +KD +S     DK   L ERAE+L+  LK RYP L QT LD  KI   KDVG ++LE+YSRVL  LAF+I++ I D+L  D
Sbjct:  352 SPEKLLDRLKIVSEHEALQLADRVEASMYTWRRKACLNNS-----KSSWNMVKDLMSITERSDKNYVLAERAESLLFCLKQRYPELSQTSLDICKIHCNKDVGKAVLESYSRVLEGLAFNIVAWIDDVLYVD 478
The following BLAST results are available for this feature:
BLAST of CF931512 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 2
Match NameE-valueIdentityDescription
ROGF1_ARATH1.239e-2543.80Rop guanine nucleotide exchange factor 1 OS=Arabid... [more]
ROGF2_ARATH1.568e-2042.42Rop guanine nucleotide exchange factor 2 OS=Arabid... [more]
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Properties
Property NameValue
Genbank descriptionEST0585 Mature peel (flavedo) cDNA subtraction library Citrus sinensis cDNA clone 6P060 similar to unknown arabidopsis protein (acc# BAC43154), mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF931512 ID=CF931512; Name=CF931512; organism=Citrus sinensis; type=EST; length=715bp
ACAAGGTCTTGACTGCTGAATCAAGTTCTTCTGGCGAAATGATCAATTTC
CTCAATTTAAAATCTGAACACAGTGCACTTGAAGCCATTAACAAGTTAGA
AGCTGCTGTATTTACGTGGAAAGAAAGAATTTCTGAACAAGCTAGTGGTA
AATCCCCCGTCCGAACATCATGGTCCTTCATTAAAGACCCTATATCAGAA
CTGGATAAGATAGAGTTCCTATTGGAACGAGCAGAAGCCCTCATACAGCA
GCTAAAGAACAGATATCCCAACCTTCCTCAAACATTTCTCGATGCCACAA
AAATCCAATACGGCAAGGATGTTGGGCATTCAATACTGGAAGCATACTCC
CGGGTTCTTGCAAACTTGGCTTTCAGCATACTGTCTAGGATAGGAGACAT
TCTACAAGAAGATGCATTGAGCAATCCCAATTCACCTGTGGCAAAATGTT
GCACTCCAGGTTCAAAAATGAATGATGATAATATGGATCAGATTCAGATG
CCATGGCTTCGTCTACGTTCAAGGCACTCGCTCATTGATCAGATGAATAA
AGCAGACGGAAAGTACCTGCCCGGGCGGCCGCCCGGGCAGGTACAACTAC
AAGAAATGGACCCGGAATTTGTTGAGCGTTCTGTAAGAAACCGAGCATTG
ACACTGACTGGACTGTTTTCCCAAGACCCATTTCATCAGCTAAAATGACA
TTGGTATCATTTCGC
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