CK932830 | Citrus Genome Database

Overview


Name	CK932830
Unique Name	CK932830
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	514

Libraries


Library Name	Type
Developing fruit juice sac at 38 DAFB	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
CK932830_ssr246	microsatellite	CK932830_ssr246:246..257.	BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig1541	contig	Csv1_Contig1541:1..515.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CK932830 vs. ExPASy Swiss-Prot
Match: TCMO_POPKI (Trans-cinnamate 4-monooxygenase OS=Populus kitakamiensis GN=CYP73A16 PE=2 SV=1)

HSP 1 Score: 241.121 bits (614), Expect = 8.874e-66
Identity = 112/129 (86.82%), Postives = 122/129 (94.57%), Query Frame = 2

Query:  128 KALQAPPGPLPVPVFGNWLQVGDDLNHRNLSDLAKKYGDVLLLRMGQRNLVVISSPDHAKEVLHTQGVEFGSRTRNVVFDIFTGKGQEMVFTVYGEHWRKMRRIMTVPFFTNKVVQQQRFNWEDEAARV 514
            K  + PPGPLPVPVFGNWLQVGDDLNHRNL+DLAKK+GD+ LLRMGQRNLVV+SSPD +KEVLHTQGVEFGSRTRNVVFDIFTGKGQ+MVFTVYGEHWRKMRRIMTVPFFTNKVVQQ R+ WE+EAA+V
Sbjct:   29 KRFKLPPGPLPVPVFGNWLQVGDDLNHRNLTDLAKKFGDIFLLRMGQRNLVVVSSPDLSKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQYRYGWEEEAAQV 157

HSP 2 Score: 29.6462 bits (65), Expect = 8.874e-66
Identity = 14/17 (82.35%), Postives = 15/17 (88.24%), Query Frame = 3

Query:   93 VVVTITVSKLRGKRFKL 143
            V+V I VSKLRGKRFKL
Sbjct:   17 VLVAILVSKLRGKRFKL 33

[Close]

BLAST of CK932830 vs. ExPASy Swiss-Prot
Match: TCMO_POPTM (Trans-cinnamate 4-monooxygenase OS=Populus tremuloides GN=CYP73A13 PE=2 SV=1)

HSP 1 Score: 241.121 bits (614), Expect = 1.157e-65
Identity = 112/129 (86.82%), Postives = 123/129 (95.35%), Query Frame = 2

Query:  128 KALQAPPGPLPVPVFGNWLQVGDDLNHRNLSDLAKKYGDVLLLRMGQRNLVVISSPDHAKEVLHTQGVEFGSRTRNVVFDIFTGKGQEMVFTVYGEHWRKMRRIMTVPFFTNKVVQQQRFNWEDEAARV 514
            K  + PPGPLPVPVFGNWLQVGDDLNHRNL+DLAKK+GD+LLLRMGQRNLVV+SSP+ +KEVLHTQGVEFGSRTRNVVFDIFTGKGQ+MVFTVYGEHWRKMRRIMTVPFFTNKVVQQ R+ WE+EAA+V
Sbjct:   29 KRFKLPPGPLPVPVFGNWLQVGDDLNHRNLTDLAKKFGDILLLRMGQRNLVVVSSPELSKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQYRYGWEEEAAQV 157

HSP 2 Score: 29.261 bits (64), Expect = 1.157e-65
Identity = 13/17 (76.47%), Postives = 15/17 (88.24%), Query Frame = 3

Query:   93 VVVTITVSKLRGKRFKL 143
            ++V I VSKLRGKRFKL
Sbjct:   17 ILVAILVSKLRGKRFKL 33

[Close]

BLAST of CK932830 vs. ExPASy Swiss-Prot
Match: TCMO_CATRO (Trans-cinnamate 4-monooxygenase OS=Catharanthus roseus GN=CYP73A4 PE=2 SV=1)

HSP 1 Score: 239.195 bits (609), Expect = 2.796e-64
Identity = 111/129 (86.05%), Postives = 121/129 (93.80%), Query Frame = 2

Query:  128 KALQAPPGPLPVPVFGNWLQVGDDLNHRNLSDLAKKYGDVLLLRMGQRNLVVISSPDHAKEVLHTQGVEFGSRTRNVVFDIFTGKGQEMVFTVYGEHWRKMRRIMTVPFFTNKVVQQQRFNWEDEAARV 514
            K  + PPGP+PVPVFGNWLQVGDDLNHRNLSD AKK+G++ LLRMGQRNLVV+SSP+ AKEVLHTQGVEFGSRTRNVVFDIFTGKGQ+MVFTVYGEHWRKMRRIMTVPFFTNKVVQQ R+ WE+EAARV
Sbjct:   29 KKFKLPPGPIPVPVFGNWLQVGDDLNHRNLSDYAKKFGEIFLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQYRYGWEEEAARV 157

HSP 2 Score: 26.5646 bits (57), Expect = 2.796e-64
Identity = 11/17 (64.71%), Postives = 14/17 (82.35%), Query Frame = 3

Query:   93 VVVTITVSKLRGKRFKL 143
            ++V   VSKLRGK+FKL
Sbjct:   17 IIVASIVSKLRGKKFKL 33

[Close]

BLAST of CK932830 vs. ExPASy Swiss-Prot
Match: TCMO_RUTGR (Trans-cinnamate 4-monooxygenase OS=Ruta graveolens GN=CYP73A2 PE=2 SV=1)

HSP 1 Score: 233.802 bits (595), Expect = 1.374e-63
Identity = 111/129 (86.05%), Postives = 119/129 (92.25%), Query Frame = 2

Query:  128 KALQAPPGPLPVPVFGNWLQVGDDLNHRNLSDLAKKYGDVLLLRMGQRNLVVISSPDHAKEVLHTQGVEFGSRTRNVVFDIFTGKGQEMVFTVYGEHWRKMRRIMTVPFFTNKVVQQQRFNWEDEAARV 514
            K  + PPGPL  PVFGNWLQVGDDLN R L++L+KK+GDV LLRMGQRNLVV+SSP+ AKEVLHTQGVEFGSRTRNVVFDIFTGKGQ+MVFTVY EHWRKMRRIMTVPFFTNKVVQQQRFNWEDEAARV
Sbjct:   29 KRFKLPPGPLGFPVFGNWLQVGDDLNQRKLANLSKKFGDVYLLRMGQRNLVVVSSPEMAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFTVYSEHWRKMRRIMTVPFFTNKVVQQQRFNWEDEAARV 157

HSP 2 Score: 29.6462 bits (65), Expect = 1.374e-63
Identity = 14/16 (87.50%), Postives = 14/16 (87.50%), Query Frame = 3

Query:   96 VVTITVSKLRGKRFKL 143
            VV I VSKLRGKRFKL
Sbjct:   18 VVAIAVSKLRGKRFKL 33

[Close]

BLAST of CK932830 vs. ExPASy Swiss-Prot
Match: TCMO_GLYEC (Trans-cinnamate 4-monooxygenase OS=Glycyrrhiza echinata GN=CYP73A14 PE=2 SV=1)

HSP 1 Score: 236.884 bits (603), Expect = 1.792e-63
Identity = 109/129 (84.50%), Postives = 120/129 (93.02%), Query Frame = 2

Query:  128 KALQAPPGPLPVPVFGNWLQVGDDLNHRNLSDLAKKYGDVLLLRMGQRNLVVISSPDHAKEVLHTQGVEFGSRTRNVVFDIFTGKGQEMVFTVYGEHWRKMRRIMTVPFFTNKVVQQQRFNWEDEAARV 514
            +  + PPGP+PVP+FGNWLQVGDDLNHRNL+DLAK++GD+ LLRMGQRNLVV+SSP+ AKEVLHTQGVEFGSRTRNVVFDIFTGKGQ+MVFTVYGEHWRKMRRIMTVPFFTNKVVQQ RF WE EAA V
Sbjct:   29 RRFKLPPGPIPVPIFGNWLQVGDDLNHRNLTDLAKRFGDIFLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQYRFGWESEAASV 157

HSP 2 Score: 26.1794 bits (56), Expect = 1.792e-63
Identity = 10/16 (62.50%), Postives = 13/16 (81.25%), Query Frame = 3

Query:   96 VVTITVSKLRGKRFKL 143
            +  I +SKLRG+RFKL
Sbjct:   18 ITAIAISKLRGRRFKL 33

[Close]

BLAST of CK932830 vs. ExPASy Swiss-Prot
Match: TCMO_PHAAU (Trans-cinnamate 4-monooxygenase OS=Phaseolus aureus GN=CYP73A2 PE=1 SV=1)

HSP 1 Score: 233.032 bits (593), Expect = 2.337e-63
Identity = 109/129 (84.50%), Postives = 118/129 (91.47%), Query Frame = 2

Query:  128 KALQAPPGPLPVPVFGNWLQVGDDLNHRNLSDLAKKYGDVLLLRMGQRNLVVISSPDHAKEVLHTQGVEFGSRTRNVVFDIFTGKGQEMVFTVYGEHWRKMRRIMTVPFFTNKVVQQQRFNWEDEAARV 514
            K  + PPGPLPVP+FGNWLQVGDDLNHRNL+ LAK++GD+ LLRMGQRNLVV+SSPD AKEVLHTQGVEFGSRTRNVVFDIFTG+GQ+MVFTVYGEHWRKMRRIMTVPFFTNKVVQQ R  WE EAA V
Sbjct:   29 KRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTQLAKRFGDIFLLRMGQRNLVVVSSPDLAKEVLHTQGVEFGSRTRNVVFDIFTGEGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQYRHGWEAEAAAV 157

HSP 2 Score: 29.6462 bits (65), Expect = 2.337e-63
Identity = 14/16 (87.50%), Postives = 14/16 (87.50%), Query Frame = 3

Query:   96 VVTITVSKLRGKRFKL 143
            VV I VSKLRGKRFKL
Sbjct:   18 VVAIVVSKLRGKRFKL 33

[Close]

BLAST of CK932830 vs. ExPASy Swiss-Prot
Match: TCMO_PETCR (Trans-cinnamate 4-monooxygenase OS=Petroselinum crispum GN=CYP73A10 PE=2 SV=1)

HSP 1 Score: 234.572 bits (597), Expect = 3.973e-63
Identity = 110/129 (85.27%), Postives = 119/129 (92.25%), Query Frame = 2

Query:  128 KALQAPPGPLPVPVFGNWLQVGDDLNHRNLSDLAKKYGDVLLLRMGQRNLVVISSPDHAKEVLHTQGVEFGSRTRNVVFDIFTGKGQEMVFTVYGEHWRKMRRIMTVPFFTNKVVQQQRFNWEDEAARV 514
            K L+ PPGP+PVPVFGNWLQVGDDLN RNL D AKK+GD+ +LRMGQRNLVV+SSP+ AK+VLHTQGVEFGSRTRNVVFDIFTGKGQ+MVFTVY EHWRKMRRIMTVPFFTNKVVQQ RF WEDEAARV
Sbjct:   30 KKLKLPPGPIPVPVFGNWLQVGDDLNQRNLVDYAKKFGDLFMLRMGQRNLVVVSSPELAKDVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFTVYSEHWRKMRRIMTVPFFTNKVVQQYRFGWEDEAARV 158

HSP 2 Score: 27.335 bits (59), Expect = 3.973e-63
Identity = 11/16 (68.75%), Postives = 14/16 (87.50%), Query Frame = 3

Query:   96 VVTITVSKLRGKRFKL 143
            +V IT+SKLRGK+ KL
Sbjct:   19 IVAITISKLRGKKLKL 34

[Close]

BLAST of CK932830 vs. ExPASy Swiss-Prot
Match: TCMO_HELTU (Trans-cinnamate 4-monooxygenase OS=Helianthus tuberosus GN=CYP73A1 PE=1 SV=1)

HSP 1 Score: 236.113 bits (601), Expect = 3.973e-63
Identity = 109/129 (84.50%), Postives = 121/129 (93.80%), Query Frame = 2

Query:  128 KALQAPPGPLPVPVFGNWLQVGDDLNHRNLSDLAKKYGDVLLLRMGQRNLVVISSPDHAKEVLHTQGVEFGSRTRNVVFDIFTGKGQEMVFTVYGEHWRKMRRIMTVPFFTNKVVQQQRFNWEDEAARV 514
            K  + PPGP+PVP+FGNWLQVGDDLNHRNL+DLAK++G++LLLRMGQRNLVV+SSP+ AKEVLHTQGVEFGSRTRNVVFDIFTGKGQ+MVFTVYGEHWRKMRRIMTVPFFTNKVVQQ R+ WE EAA V
Sbjct:   29 KKFKLPPGPIPVPIFGNWLQVGDDLNHRNLTDLAKRFGEILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQYRYGWEAEAAAV 157

HSP 2 Score: 25.7942 bits (55), Expect = 3.973e-63
Identity = 10/17 (58.82%), Postives = 14/17 (82.35%), Query Frame = 3

Query:   93 VVVTITVSKLRGKRFKL 143
            ++  I +SKLRGK+FKL
Sbjct:   17 IIGAILISKLRGKKFKL 33

[Close]

BLAST of CK932830 vs. ExPASy Swiss-Prot
Match: TCMO_SOYBN (Trans-cinnamate 4-monooxygenase OS=Glycine max GN=CYP73A11 PE=2 SV=1)

HSP 1 Score: 236.113 bits (601), Expect = 6.755e-63
Identity = 110/129 (85.27%), Postives = 119/129 (92.25%), Query Frame = 2

Query:  128 KALQAPPGPLPVPVFGNWLQVGDDLNHRNLSDLAKKYGDVLLLRMGQRNLVVISSPDHAKEVLHTQGVEFGSRTRNVVFDIFTGKGQEMVFTVYGEHWRKMRRIMTVPFFTNKVVQQQRFNWEDEAARV 514
            +  + PPGPLPVP+FGNWLQVGDDLNHRNL+DLAKK+GD+ LLRMGQRNLVV+SSP+ AKEVLHTQGVEFGSRTRNVVFDIFTGKGQ+MVFTVYGEHWRKMRRIMTVPFFTNKVVQQ R  WE EAA V
Sbjct:   29 RKFKLPPGPLPVPIFGNWLQVGDDLNHRNLTDLAKKFGDIFLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQYRHGWESEAAAV 157

HSP 2 Score: 25.0238 bits (53), Expect = 6.755e-63
Identity = 11/16 (68.75%), Postives = 13/16 (81.25%), Query Frame = 3

Query:   96 VVTITVSKLRGKRFKL 143
            VV I VS LRG++FKL
Sbjct:   18 VVAIAVSTLRGRKFKL 33

[Close]

BLAST of CK932830 vs. ExPASy Swiss-Prot
Match: TCMO_ZINEL (Trans-cinnamate 4-monooxygenase OS=Zinnia elegans GN=CYP73A12 PE=2 SV=1)

HSP 1 Score: 233.032 bits (593), Expect = 6.756e-63
Identity = 109/129 (84.50%), Postives = 119/129 (92.25%), Query Frame = 2

Query:  128 KALQAPPGPLPVPVFGNWLQVGDDLNHRNLSDLAKKYGDVLLLRMGQRNLVVISSPDHAKEVLHTQGVEFGSRTRNVVFDIFTGKGQEMVFTVYGEHWRKMRRIMTVPFFTNKVVQQQRFNWEDEAARV 514
            K  + PPGP+PVP+FGNWLQVGDDLNHRNL+DLAKK+G++ LLRMGQRNLVV+SSP+ AKEVLHTQGVEFGSRTRNVVFDIFTGKGQ+MVFTVYGEHWRKMRRIMTVPFFTNKVVQQ R  WE EAA V
Sbjct:   29 KRFKLPPGPVPVPIFGNWLQVGDDLNHRNLTDLAKKFGEIFLLRMGQRNLVVVSSPNLAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQYRTGWEAEAAAV 157

HSP 2 Score: 28.1054 bits (61), Expect = 6.756e-63
Identity = 11/17 (64.71%), Postives = 15/17 (88.24%), Query Frame = 3

Query:   93 VVVTITVSKLRGKRFKL 143
            ++ +I +SKLRGKRFKL
Sbjct:   17 IIASIFISKLRGKRFKL 33

The following BLAST results are available for this feature:

BLAST of CK932830 vs. ExPASy Swiss-Prot

BLAST of CK932830 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 110


Match Name	E-value	Identity	Description
TCMO_POPKI	8.874e-66	86.82	Trans-cinnamate 4-monooxygenase OS=Populus kitakam...	[more]
TCMO_POPTM	1.157e-65	86.82	Trans-cinnamate 4-monooxygenase OS=Populus tremulo...	[more]
TCMO_CATRO	2.796e-64	86.05	Trans-cinnamate 4-monooxygenase OS=Catharanthus ro...	[more]
TCMO_RUTGR	1.374e-63	86.05	Trans-cinnamate 4-monooxygenase OS=Ruta graveolens...	[more]
TCMO_GLYEC	1.792e-63	84.50	Trans-cinnamate 4-monooxygenase OS=Glycyrrhiza ech...	[more]
TCMO_PHAAU	2.337e-63	84.50	Trans-cinnamate 4-monooxygenase OS=Phaseolus aureu...	[more]
TCMO_PETCR	3.973e-63	85.27	Trans-cinnamate 4-monooxygenase OS=Petroselinum cr...	[more]
TCMO_HELTU	3.973e-63	84.50	Trans-cinnamate 4-monooxygenase OS=Helianthus tube...	[more]
TCMO_SOYBN	6.755e-63	85.27	Trans-cinnamate 4-monooxygenase OS=Glycine max GN=...	[more]
TCMO_ZINEL	6.756e-63	84.50	Trans-cinnamate 4-monooxygenase OS=Zinnia elegans ...	[more]

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Properties


Property Name	Value
Genbank description	CGF1004529_A08 Developing fruit juice sac at 38 DAFB Citrus sinensis cDNA clone JSJune0001_IIF_A08 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK932830 ID=CK932830; Name=CK932830; organism=Citrus sinensis; type=EST; length=514bp
GGGGACCCACTGGACTTTTTGTATTCATTACGTTAATCGTAANNNTGGAT
CTCCTCTTGCTGGAAAAGACGCTTTTAGCCCTCTTCGCCGCCGTTGTTGT
CACCATTACCGTCTCTAAACTCCGCGGAAAGCGCTTCAAGCTCCCCCCGG
CCCCCTCCCCGTCCCCGTATTCGGCAACTGGCTCCAGGTCGGCGATGACT
TGAACCATCGGAACCTCTCCGACTTAGCCAAGAAATATGGAGATGTCCTC
CTCCTCCGCATGGGCCAGCGCAACCTCGTCGTCATCTCGTCGCCGGACCA
CGCCAAGGAGGTTCTGCACACGCAGGGAGTGGAGTTCGGATCACGAACGA
GGAACGTCGTGTTCGATATCTTCACTGGGAAGGGCCAGGAGATGGTGTTC
ACAGTGTACGGCGAGCACTGGCGGAAGATGCGGAGGATCATGACCGTCCC
TTTCTTCACGAACAAAGTGGTTCAGCAGCAGCGGTTTAATTGGGAAGACG
AGGCCGCTAGAGTC