EY736438

Overview
NameEY736438
Unique NameEY736438
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length749
Libraries
Library NameType
Sweet orange fruit, development stadium (6 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY736438 vs. ExPASy Swiss-Prot
Match: GLNA_VIGAC (Glutamine synthetase nodule isozyme OS=Vigna aconitifolia PE=2 SV=1)

HSP 1 Score: 261.536 bits (667), Expect = 1.566e-70
Identity = 125/154 (81.17%), Postives = 135/154 (87.66%), Query Frame = 2 
Query:  128 TDKIIAEYIWIGGSGMDMRSKARTLPGPVSDPSKLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAARIFSHSDVVGEEPWYGIEQE*PYCRTMLNGLLGGPSVGYPGAQGPYYWWGVGPDQAWG 589
            T+KIIAEYIWIGGSG+D+RSKARTLPGPVSDPSKLPKWNYDGSSTGQAPGEDSEVI+YPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRH AA+IFSH DVV EEPWYGIEQE    +  +N  LG P  G+PG QGPYY  G G D+A+G
Sbjct:   16 TEKIIAEYIWIGGSGLDLRSKARTLPGPVSDPSKLPKWNYDGSSTGQAPGEDSEVIIYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHNAAKIFSHPDVVAEEPWYGIEQEYTLLQKDVNWPLGWPVGGFPGPQGPYY-CGAGADKAFG 168

HSP 2 Score: 26.5646 bits (57), Expect = 1.566e-70
Identity = 13/23 (56.52%), Postives = 15/23 (65.22%), Query Frame = 3 
Query:  570 GRTKLGGRDMGDPHYKAGL*AGM 638
            G  K  GRD+ D HYKA L AG+
Sbjct:  162 GADKAFGRDIVDAHYKACLYAGI 184
[Close]
BLAST of EY736438 vs. ExPASy Swiss-Prot
Match: GLNA1_MEDSA (Glutamine synthetase cytosolic isozyme OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 258.84 bits (660), Expect = 1.005e-69
Identity = 123/154 (79.87%), Postives = 135/154 (87.66%), Query Frame = 2 
Query:  128 TDKIIAEYIWIGGSGMDMRSKARTLPGPVSDPSKLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAARIFSHSDVVGEEPWYGIEQE*PYCRTMLNGLLGGPSVGYPGAQGPYYWWGVGPDQAWG 589
            T+KIIAEYIWIGGSG+D+RSKARTLPGPV+DPS+LPKWNYDGSSTGQAPGEDSEVI+YPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAA+IFSH DVV E PWYGIEQE    +  +N  LG P  G+PG QGPYY  G G D+A+G
Sbjct:   16 TEKIIAEYIWIGGSGLDLRSKARTLPGPVTDPSQLPKWNYDGSSTGQAPGEDSEVIIYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAAKIFSHPDVVAEVPWYGIEQEYTLLQKDINWPLGWPVGGFPGPQGPYY-CGAGADKAFG 168

HSP 2 Score: 26.5646 bits (57), Expect = 1.005e-69
Identity = 13/23 (56.52%), Postives = 15/23 (65.22%), Query Frame = 3 
Query:  570 GRTKLGGRDMGDPHYKAGL*AGM 638
            G  K  GRD+ D HYKA L AG+
Sbjct:  162 GADKAFGRDIVDSHYKACLYAGI 184
[Close]
BLAST of EY736438 vs. ExPASy Swiss-Prot
Match: GLNA1_LOTJA (Glutamine synthetase cytosolic isozyme OS=Lotus japonicus GN=GLN1 PE=2 SV=2)

HSP 1 Score: 258.455 bits (659), Expect = 1.310e-69
Identity = 122/154 (79.22%), Postives = 136/154 (88.31%), Query Frame = 2 
Query:  128 TDKIIAEYIWIGGSGMDMRSKARTLPGPVSDPSKLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAARIFSHSDVVGEEPWYGIEQE*PYCRTMLNGLLGGPSVGYPGAQGPYYWWGVGPDQAWG 589
            TDKIIAEYIWIGGSG+DMRSKARTLPGPVSDPS+LPKWNYDGSSTGQAPGEDSEVILYPQAIF+DPFRRG+NILV+CDAYTPAGEPIPTNKRHAAA++FSH DVV E PWYGIEQE    +  +N  +G P  G+PG QGPYY  G+G D+A+G
Sbjct:   16 TDKIIAEYIWIGGSGLDMRSKARTLPGPVSDPSQLPKWNYDGSSTGQAPGEDSEVILYPQAIFRDPFRRGSNILVICDAYTPAGEPIPTNKRHAAAKVFSHPDVVAEVPWYGIEQEYTLLQKEVNWPVGWPIGGFPGPQGPYY-CGIGADKAFG 168

HSP 2 Score: 26.5646 bits (57), Expect = 1.310e-69
Identity = 13/23 (56.52%), Postives = 15/23 (65.22%), Query Frame = 3 
Query:  570 GRTKLGGRDMGDPHYKAGL*AGM 638
            G  K  GRD+ D HYKA L AG+
Sbjct:  162 GADKAFGRDIVDAHYKACLYAGV 184
[Close]
BLAST of EY736438 vs. ExPASy Swiss-Prot
Match: GLNA_NICPL (Glutamine synthetase OS=Nicotiana plumbaginifolia PE=2 SV=1)

HSP 1 Score: 258.07 bits (658), Expect = 1.709e-69
Identity = 124/154 (80.52%), Postives = 135/154 (87.66%), Query Frame = 2 
Query:  128 TDKIIAEYIWIGGSGMDMRSKARTLPGPVSDPSKLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAARIFSHSDVVGEEPWYGIEQE*PYCRTMLNGLLGGPSVGYPGAQGPYYWWGVGPDQAWG 589
            T+KIIAEYIWIGGSGMD+RSKARTL GPV+DP+KLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAA+IFS+ DVV EEPWYGIEQE    +  +N  LG P  G+PG QGPYY  G G D+A+G
Sbjct:   16 TEKIIAEYIWIGGSGMDLRSKARTLSGPVTDPAKLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAAKIFSNPDVVAEEPWYGIEQEYTLLQRDINWPLGWPIGGFPGPQGPYY-CGTGADKAFG 168

HSP 2 Score: 26.5646 bits (57), Expect = 1.709e-69
Identity = 13/23 (56.52%), Postives = 15/23 (65.22%), Query Frame = 3 
Query:  570 GRTKLGGRDMGDPHYKAGL*AGM 638
            G  K  GRD+ D HYKA L AG+
Sbjct:  162 GADKAFGRDIVDSHYKAYLYAGI 184
[Close]
BLAST of EY736438 vs. ExPASy Swiss-Prot
Match: GLN11_ARATH (Glutamine synthetase cytosolic isozyme 1-1 OS=Arabidopsis thaliana GN=GLN1-1 PE=1 SV=2)

HSP 1 Score: 256.144 bits (653), Expect = 8.405e-69
Identity = 121/154 (78.57%), Postives = 134/154 (87.01%), Query Frame = 2 
Query:  128 TDKIIAEYIWIGGSGMDMRSKARTLPGPVSDPSKLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAARIFSHSDVVGEEPWYGIEQE*PYCRTMLNGLLGGPSVGYPGAQGPYYWWGVGPDQAWG 589
            TDKIIAEYIW+GGSGMDMRSKARTLPGPV+DPS+LPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAA++FS+ DV  E PWYGIEQE    +  +   +G P  GYPG QGPYY  G+G D+++G
Sbjct:   16 TDKIIAEYIWVGGSGMDMRSKARTLPGPVTDPSQLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAAKVFSNPDVAAEVPWYGIEQEYTLLQKDVKWPVGWPIGGYPGPQGPYY-CGIGADKSFG 168

HSP 2 Score: 26.1794 bits (56), Expect = 8.405e-69
Identity = 13/23 (56.52%), Postives = 15/23 (65.22%), Query Frame = 3 
Query:  570 GRTKLGGRDMGDPHYKAGL*AGM 638
            G  K  GRD+ D HYKA L AG+
Sbjct:  162 GADKSFGRDVVDSHYKACLYAGI 184
[Close]
BLAST of EY736438 vs. ExPASy Swiss-Prot
Match: GLN13_ARATH (Glutamine synthetase cytosolic isozyme 1-3 OS=Arabidopsis thaliana GN=GLN1-3 PE=1 SV=1)

HSP 1 Score: 253.832 bits (647), Expect = 4.134e-68
Identity = 123/154 (79.87%), Postives = 132/154 (85.71%), Query Frame = 2 
Query:  128 TDKIIAEYIWIGGSGMDMRSKARTLPGPVSDPSKLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAARIFSHSDVVGEEPWYGIEQE*PYCRTMLNGLLGGPSVGYPGAQGPYYWWGVGPDQAWG 589
            T KIIAEYIWIGGSGMD+RSKARTLPGPV+DPSKLPKWNYDGSSTGQA GEDSEVILYPQAIFKDPFR+GNNILVMCDAYTPAG+PIPTNKRH AA+IFSH DV  EEPWYGIEQE    +  +N  +G P  GYPG QGPYY  GVG D+A G
Sbjct:   16 TGKIIAEYIWIGGSGMDIRSKARTLPGPVTDPSKLPKWNYDGSSTGQAAGEDSEVILYPQAIFKDPFRKGNNILVMCDAYTPAGDPIPTNKRHNAAKIFSHPDVAKEEPWYGIEQEYTLMQKDVNWPIGWPVGGYPGPQGPYY-CGVGADKAIG 168

HSP 2 Score: 26.1794 bits (56), Expect = 4.134e-68
Identity = 13/23 (56.52%), Postives = 15/23 (65.22%), Query Frame = 3 
Query:  570 GRTKLGGRDMGDPHYKAGL*AGM 638
            G  K  GRD+ D HYKA L AG+
Sbjct:  162 GADKAIGRDIVDAHYKACLYAGI 184
[Close]
BLAST of EY736438 vs. ExPASy Swiss-Prot
Match: GLN12_ARATH (Glutamine synthetase cytosolic isozyme 1-2 OS=Arabidopsis thaliana GN=GLN1-2 PE=1 SV=2)

HSP 1 Score: 252.677 bits (644), Expect = 4.134e-68
Identity = 119/154 (77.27%), Postives = 134/154 (87.01%), Query Frame = 2 
Query:  128 TDKIIAEYIWIGGSGMDMRSKARTLPGPVSDPSKLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAARIFSHSDVVGEEPWYGIEQE*PYCRTMLNGLLGGPSVGYPGAQGPYYWWGVGPDQAWG 589
            ++KIIAEYIW+GGSGMDMRSKARTLPGPV+DPSKLPKWNYDGSSTGQAPG+DSEVILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAA IF++ DV+ E PWYGIEQE    +  +N  LG P  G+PG QGPYY   +G D+++G
Sbjct:   16 SEKIIAEYIWVGGSGMDMRSKARTLPGPVTDPSKLPKWNYDGSSTGQAPGQDSEVILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAAEIFANPDVIAEVPWYGIEQEYTLLQKDVNWPLGWPIGGFPGPQGPYY-CSIGADKSFG 168

HSP 2 Score: 27.335 bits (59), Expect = 4.134e-68
Identity = 13/23 (56.52%), Postives = 15/23 (65.22%), Query Frame = 3 
Query:  570 GRTKLGGRDMGDPHYKAGL*AGM 638
            G  K  GRD+ D HYKA L AG+
Sbjct:  162 GADKSFGRDIVDAHYKASLYAGI 184
[Close]
BLAST of EY736438 vs. ExPASy Swiss-Prot
Match: GLNA1_PEA (Glutamine synthetase nodule isozyme OS=Pisum sativum GN=GS1 PE=2 SV=2)

HSP 1 Score: 254.218 bits (648), Expect = 7.030e-68
Identity = 120/154 (77.92%), Postives = 135/154 (87.66%), Query Frame = 2 
Query:  128 TDKIIAEYIWIGGSGMDMRSKARTLPGPVSDPSKLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAARIFSHSDVVGEEPWYGIEQE*PYCRTMLNGLLGGPSVGYPGAQGPYYWWGVGPDQAWG 589
            T+KIIAEYIWIGGSG+D+R KARTLPGPV+DPS+LPKWNYDGSSTGQAPG+DSEVILYPQAIFKDPFRRGN+ILVMCDAY+PAGEPIPTNKRHAAA++FSH DVV EE WYGIEQE    +  +N  LG P+ GYPG QGPYY   VG D+A+G
Sbjct:   15 TEKIIAEYIWIGGSGLDLRCKARTLPGPVTDPSELPKWNYDGSSTGQAPGQDSEVILYPQAIFKDPFRRGNHILVMCDAYSPAGEPIPTNKRHAAAKVFSHPDVVAEETWYGIEQEYTLLQKDINWPLGWPAGGYPGPQGPYY-CSVGADKAFG 167

HSP 2 Score: 25.0238 bits (53), Expect = 7.030e-68
Identity = 12/23 (52.17%), Postives = 15/23 (65.22%), Query Frame = 3 
Query:  570 GRTKLGGRDMGDPHYKAGL*AGM 638
            G  K  GRD+ + HYKA L AG+
Sbjct:  161 GADKAFGRDVVEAHYKACLFAGI 183
[Close]
BLAST of EY736438 vs. ExPASy Swiss-Prot
Match: GLNA2_VITVI (Glutamine synthetase cytosolic isozyme 2 OS=Vitis vinifera GN=GS1-2 PE=2 SV=1)

HSP 1 Score: 251.906 bits (642), Expect = 1.196e-67
Identity = 120/154 (77.92%), Postives = 131/154 (85.06%), Query Frame = 2 
Query:  128 TDKIIAEYIWIGGSGMDMRSKARTLPGPVSDPSKLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAARIFSHSDVVGEEPWYGIEQE*PYCRTMLNGLLGGPSVGYPGAQGPYYWWGVGPDQAWG 589
            T+KIIAEYIWIGGSGMD+RSKARTL GPVSDP KLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCD YTPAGEPIPTNKRH AA+IFSH +V+ EE WYGIEQE    +  +   +G P  GYPG QGPYY  G+G D+A+G
Sbjct:   16 TEKIIAEYIWIGGSGMDLRSKARTLSGPVSDPHKLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDTYTPAGEPIPTNKRHNAAKIFSHPEVLAEETWYGIEQEYTLLQNSVKWPIGWPVGGYPGPQGPYY-CGIGADKAFG 168

HSP 2 Score: 26.5646 bits (57), Expect = 1.196e-67
Identity = 13/23 (56.52%), Postives = 15/23 (65.22%), Query Frame = 3 
Query:  570 GRTKLGGRDMGDPHYKAGL*AGM 638
            G  K  GRD+ D HYKA L AG+
Sbjct:  162 GADKAFGRDIVDSHYKACLYAGI 184
[Close]
BLAST of EY736438 vs. ExPASy Swiss-Prot
Match: GLNA1_PHAVU (Glutamine synthetase PR-1 OS=Phaseolus vulgaris PE=2 SV=1)

HSP 1 Score: 251.521 bits (641), Expect = 3.457e-67
Identity = 118/154 (76.62%), Postives = 135/154 (87.66%), Query Frame = 2 
Query:  128 TDKIIAEYIWIGGSGMDMRSKARTLPGPVSDPSKLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAARIFSHSDVVGEEPWYGIEQE*PYCRTMLNGLLGGPSVGYPGAQGPYYWWGVGPDQAWG 589
            T+K+IAEYIWIGGSG+D+RSKARTLPGPV +PS+LPKWNYDGSSTGQAPG+DSEVI+YPQAIFKDPFRRGNNILV+CDAYTPAGEPIPTNKRH AA+IFS+ DVV EEPWYGIEQE    +  +N  +G P  G+PG QGPYY  GVG D+A+G
Sbjct:   16 TEKVIAEYIWIGGSGLDLRSKARTLPGPVKNPSELPKWNYDGSSTGQAPGQDSEVIIYPQAIFKDPFRRGNNILVICDAYTPAGEPIPTNKRHNAAKIFSNPDVVAEEPWYGIEQEYTLLQKEVNWPVGWPVGGFPGPQGPYY-CGVGADKAFG 168

HSP 2 Score: 25.409 bits (54), Expect = 3.457e-67
Identity = 12/23 (52.17%), Postives = 15/23 (65.22%), Query Frame = 3 
Query:  570 GRTKLGGRDMGDPHYKAGL*AGM 638
            G  K  GRD+ D HYKA + AG+
Sbjct:  162 GADKAFGRDIVDAHYKACVYAGI 184
The following BLAST results are available for this feature:
BLAST of EY736438 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 87
Match NameE-valueIdentityDescription
GLNA_VIGAC1.566e-7081.17Glutamine synthetase nodule isozyme OS=Vigna aconi... [more]
GLNA1_MEDSA1.005e-6979.87Glutamine synthetase cytosolic isozyme OS=Medicago... [more]
GLNA1_LOTJA1.310e-6979.22Glutamine synthetase cytosolic isozyme OS=Lotus ja... [more]
GLNA_NICPL1.709e-6980.52Glutamine synthetase OS=Nicotiana plumbaginifolia ... [more]
GLN11_ARATH8.405e-6978.57Glutamine synthetase cytosolic isozyme 1-1 OS=Arab... [more]
GLN13_ARATH4.134e-6879.87Glutamine synthetase cytosolic isozyme 1-3 OS=Arab... [more]
GLN12_ARATH4.134e-6877.27Glutamine synthetase cytosolic isozyme 1-2 OS=Arab... [more]
GLNA1_PEA7.030e-6877.92Glutamine synthetase nodule isozyme OS=Pisum sativ... [more]
GLNA2_VITVI1.196e-6777.92Glutamine synthetase cytosolic isozyme 2 OS=Vitis ... [more]
GLNA1_PHAVU3.457e-6776.62Glutamine synthetase PR-1 OS=Phaseolus vulgaris PE... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-705-016-D01-CT.F Sweet orange fruit, development stadium (6 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY736438 ID=EY736438; Name=EY736438; organism=Citrus sinensis; type=EST; length=749bp
CCACGCGTCCGCATCCCTTCAATCACAGTAATCTCATTACCAGTTTCTTT
GTTGTGTTAAGAGACTAAAGAGTCTTCGAAACATGTCTCTGCTCTCAGAT
CTCCTTAACCTTAACCTCTCAGAATCCACTGACAAGATCATCGCTGAGTA
CATATGGATCGGTGGATCTGGCATGGACATGAGGAGCAAAGCAAGGACTC
TTCCCGGTCCCGTTAGTGATCCTTCCAAGCTGCCTAAGTGGAATTATGAT
GGTTCCAGCACAGGCCAAGCACCTGGAGAAGATAGTGAAGTGATCCTCTA
TCCTCAAGCTATTTTCAAGGATCCATTCAGGAGAGGCAACAATATTCTTG
TTATGTGTGATGCTTACACTCCTGCGGGGGAACCAATTCCAACAAACAAA
AGACATGCAGCTGCCAGGATTTTCAGCCATTCTGACGTTGTGGGTGAAGA
ACCATGGTATGGTATTGAGCAGGAATAACCTTACTGCAGAACGATGTTAA
ATGGCCTCTTGGGTGGCCCATCCGTGGGTTATCCTGGGGCTCAGGGACCA
TACTACTGGTGGGGTGTGGGGCCGGACCAAGCTTGGGGGGCGCGACATGG
GGGACCCCCATTACAAGGCCGGTCTATAGGCGGGGATGAGCGTTGGGGGG
GGGGAGGGGGAAAGTTGGCCGGGGCCAGGGGGAGTTCCAAGTGCGCTCCC
CGCGCGGGGATAGTCCGCGGAGGAGTGGGTGGGGCGGGTGCGGGCGGCC
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