EY736230

Overview
NameEY736230
Unique NameEY736230
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length827
Libraries
Library NameType
Sweet orange fruit, development stadium (6 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY736230 vs. ExPASy Swiss-Prot
Match: ARD4_ARATH (1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 4 OS=Arabidopsis thaliana GN=ARD4 PE=2 SV=1)

HSP 1 Score: 274.633 bits (701), Expect = 8.167e-88
Identity = 123/149 (82.55%), Postives = 138/149 (92.62%), Query Frame = 3 
Query:   45 MALEAWFMDESSQDQRLPHHRNPKQFVSLDQLAELGVLYWRLNPENYENDEELQKIREARGYSYMDLLDLCPEKVTNYEEKLKNFYTEHIHADEEIRYCLEGSGYFDVRDESDQWIRIWIKAGDLIVLPAGIYHRFTLDTSNYVKVMQI 491
            MALEAWFMD+S++DQRLPHHRNPK+ VSLD LAELGVLYW+LNPENYEND EL KIRE RGY YMDLLDLCPEKV+NYEEKLKNF+TEHIH DEEIRYCL GSGYFDVRD+ D+WIRIW++ GDLIVLPAGIYHRFTLD SNY+K+M++
Sbjct:    1 MALEAWFMDDSNEDQRLPHHRNPKELVSLDYLAELGVLYWKLNPENYENDSELSKIREDRGYDYMDLLDLCPEKVSNYEEKLKNFFTEHIHKDEEIRYCLAGSGYFDVRDKDDRWIRIWMQPGDLIVLPAGIYHRFTLDASNYIKLMRL 149

HSP 2 Score: 69.3218 bits (168), Expect = 8.167e-88
Identity = 30/44 (68.18%), Postives = 36/44 (81.82%), Query Frame = 2 
Query:  578 VQLMRLFVGEPVWTAYNRPQEDHPARKEYIKGLTGQAGVPXEAH 709
            ++LMRLFVGEPVWT YNRPQE+HP RK+YI GLT + G   +AH
Sbjct:  144 IKLMRLFVGEPVWTPYNRPQEEHPVRKKYIHGLTYKFGETVKAH 187
[Close]
BLAST of EY736230 vs. ExPASy Swiss-Prot
Match: ARD2_ORYSJ (1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 2 OS=Oryza sativa subsp. japonica GN=ARD2 PE=2 SV=1)

HSP 1 Score: 241.506 bits (615), Expect = 1.662e-73
Identity = 102/147 (69.39%), Postives = 130/147 (88.44%), Query Frame = 3 
Query:   51 LEAWFMDESSQDQRLPHHRNPKQFVSLDQLAELGVLYWRLNPENYENDEELQKIREARGYSYMDLLDLCPEKVTNYEEKLKNFYTEHIHADEEIRYCLEGSGYFDVRDESDQWIRIWIKAGDLIVLPAGIYHRFTLDTSNYVKVMQI 491
            +EAW+MD+S +DQRLPHHR PK+F+ L +L+ELG+L WRLN +++ENDE L+KIREARGYSYMD+ D+CPEK+ NYE KLKNF+ EH+H DEEIRYCLEGSGYFDVRD++DQWIR+ +K G +IVLPAG+YHRFTLD+ NY+K M++
Sbjct:   13 IEAWYMDDSEEDQRLPHHREPKEFIPLSKLSELGILSWRLNADDWENDENLKKIREARGYSYMDICDVCPEKLPNYEAKLKNFFEEHLHTDEEIRYCLEGSGYFDVRDQNDQWIRVAVKKGGMIVLPAGMYHRFTLDSDNYIKAMRL 159

HSP 2 Score: 54.6842 bits (130), Expect = 1.662e-73
Identity = 22/38 (57.89%), Postives = 29/38 (76.32%), Query Frame = 2 
Query:  578 VQLMRLFVGEPVWTAYNRPQEDHPARKEYIKGLTGQAG 691
            ++ MRLFVGEPVWT YNRP +  PARKEY++ +  + G
Sbjct:  154 IKAMRLFVGEPVWTPYNRPHDHLPARKEYVEKIINRGG 191
[Close]
BLAST of EY736230 vs. ExPASy Swiss-Prot
Match: ARD2_ORYSI (1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 2 OS=Oryza sativa subsp. indica GN=ARD2 PE=2 SV=2)

HSP 1 Score: 241.506 bits (615), Expect = 1.662e-73
Identity = 102/147 (69.39%), Postives = 130/147 (88.44%), Query Frame = 3 
Query:   51 LEAWFMDESSQDQRLPHHRNPKQFVSLDQLAELGVLYWRLNPENYENDEELQKIREARGYSYMDLLDLCPEKVTNYEEKLKNFYTEHIHADEEIRYCLEGSGYFDVRDESDQWIRIWIKAGDLIVLPAGIYHRFTLDTSNYVKVMQI 491
            +EAW+MD+S +DQRLPHHR PK+F+ L +L+ELG+L WRLN +++ENDE L+KIREARGYSYMD+ D+CPEK+ NYE KLKNF+ EH+H DEEIRYCLEGSGYFDVRD++DQWIR+ +K G +IVLPAG+YHRFTLD+ NY+K M++
Sbjct:   13 IEAWYMDDSEEDQRLPHHREPKEFIPLSKLSELGILSWRLNADDWENDENLKKIREARGYSYMDICDVCPEKLPNYEAKLKNFFEEHLHTDEEIRYCLEGSGYFDVRDQNDQWIRVAVKKGGMIVLPAGMYHRFTLDSDNYIKAMRL 159

HSP 2 Score: 54.6842 bits (130), Expect = 1.662e-73
Identity = 22/38 (57.89%), Postives = 29/38 (76.32%), Query Frame = 2 
Query:  578 VQLMRLFVGEPVWTAYNRPQEDHPARKEYIKGLTGQAG 691
            ++ MRLFVGEPVWT YNRP +  PARKEY++ +  + G
Sbjct:  154 IKAMRLFVGEPVWTPYNRPHDHLPARKEYVEKIINRGG 191
[Close]
BLAST of EY736230 vs. ExPASy Swiss-Prot
Match: ARD1_ORYSJ (1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 1 OS=Oryza sativa subsp. japonica GN=ARD1 PE=2 SV=1)

HSP 1 Score: 239.58 bits (610), Expect = 1.518e-71
Identity = 101/147 (68.71%), Postives = 130/147 (88.44%), Query Frame = 3 
Query:   51 LEAWFMDESSQDQRLPHHRNPKQFVSLDQLAELGVLYWRLNPENYENDEELQKIREARGYSYMDLLDLCPEKVTNYEEKLKNFYTEHIHADEEIRYCLEGSGYFDVRDESDQWIRIWIKAGDLIVLPAGIYHRFTLDTSNYVKVMQI 491
            +EAW+MD+S +DQRLPHHR PK+F+ +D+L ELGV+ WRLNP+N+EN E L++IREARGYSY+D+ D+CPEK+ NYE K+K+F+ EH+H DEEIRYCLEGSGYFDVRD++DQWIRI +K G +IVLPAG+YHRFTLDT NY+K M++
Sbjct:   13 IEAWYMDDSEEDQRLPHHREPKEFIHVDKLTELGVISWRLNPDNWENCENLKRIREARGYSYVDICDVCPEKLPNYETKIKSFFEEHLHTDEEIRYCLEGSGYFDVRDQNDQWIRIALKKGGMIVLPAGMYHRFTLDTDNYIKAMRL 159

HSP 2 Score: 50.0618 bits (118), Expect = 1.518e-71
Identity = 21/38 (55.26%), Postives = 27/38 (71.05%), Query Frame = 2 
Query:  578 VQLMRLFVGEPVWTAYNRPQEDHPARKEYIKGLTGQAG 691
            ++ MRLFVG+PVWT YNRP +  PARKE++  L    G
Sbjct:  154 IKAMRLFVGDPVWTPYNRPHDHLPARKEFLAKLLKSEG 191
[Close]
BLAST of EY736230 vs. ExPASy Swiss-Prot
Match: ARD1_ORYSI (1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 1 OS=Oryza sativa subsp. indica GN=ARD1 PE=1 SV=2)

HSP 1 Score: 239.58 bits (610), Expect = 1.518e-71
Identity = 101/147 (68.71%), Postives = 130/147 (88.44%), Query Frame = 3 
Query:   51 LEAWFMDESSQDQRLPHHRNPKQFVSLDQLAELGVLYWRLNPENYENDEELQKIREARGYSYMDLLDLCPEKVTNYEEKLKNFYTEHIHADEEIRYCLEGSGYFDVRDESDQWIRIWIKAGDLIVLPAGIYHRFTLDTSNYVKVMQI 491
            +EAW+MD+S +DQRLPHHR PK+F+ +D+L ELGV+ WRLNP+N+EN E L++IREARGYSY+D+ D+CPEK+ NYE K+K+F+ EH+H DEEIRYCLEGSGYFDVRD++DQWIRI +K G +IVLPAG+YHRFTLDT NY+K M++
Sbjct:   13 IEAWYMDDSEEDQRLPHHREPKEFIHVDKLTELGVISWRLNPDNWENCENLKRIREARGYSYVDICDVCPEKLPNYETKIKSFFEEHLHTDEEIRYCLEGSGYFDVRDQNDQWIRIALKKGGMIVLPAGMYHRFTLDTDNYIKAMRL 159

HSP 2 Score: 50.0618 bits (118), Expect = 1.518e-71
Identity = 21/38 (55.26%), Postives = 27/38 (71.05%), Query Frame = 2 
Query:  578 VQLMRLFVGEPVWTAYNRPQEDHPARKEYIKGLTGQAG 691
            ++ MRLFVG+PVWT YNRP +  PARKE++  L    G
Sbjct:  154 IKAMRLFVGDPVWTPYNRPHDHLPARKEFLAKLLKSEG 191
[Close]
BLAST of EY736230 vs. ExPASy Swiss-Prot
Match: ARD3_ARATH (1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 3 OS=Arabidopsis thaliana GN=ARD3 PE=2 SV=1)

HSP 1 Score: 235.343 bits (599), Expect = 5.728e-71
Identity = 97/147 (65.99%), Postives = 130/147 (88.44%), Query Frame = 3 
Query:   51 LEAWFMDESSQDQRLPHHRNPKQFVSLDQLAELGVLYWRLNPENYENDEELQKIREARGYSYMDLLDLCPEKVTNYEEKLKNFYTEHIHADEEIRYCLEGSGYFDVRDESDQWIRIWIKAGDLIVLPAGIYHRFTLDTSNYVKVMQI 491
            ++AW+MD+S +DQRLPHH++PK+F+SLD+LAELGVL WRL+ +NYE DE+L+KIRE+RGYSYMD  ++CPEK+ NYE K+K+F+ EH+H DEEIRYC+ GSGYFDVRD ++ WIR+W+K G +IVLPAGIYHRFT+D+ NY+K M++
Sbjct:   13 IQAWYMDDSEEDQRLPHHKDPKEFLSLDKLAELGVLSWRLDADNYETDEDLKKIRESRGYSYMDFCEVCPEKLPNYEVKVKSFFEEHLHTDEEIRYCVAGSGYFDVRDRNEAWIRVWVKKGGMIVLPAGIYHRFTVDSDNYIKAMRL 159

HSP 2 Score: 52.373 bits (124), Expect = 5.728e-71
Identity = 22/30 (73.33%), Postives = 25/30 (83.33%), Query Frame = 2 
Query:  578 VQLMRLFVGEPVWTAYNRPQEDHPARKEYI 667
            ++ MRLFVGEPVWT YNRP +  PARKEYI
Sbjct:  154 IKAMRLFVGEPVWTPYNRPHDHLPARKEYI 183
[Close]
BLAST of EY736230 vs. ExPASy Swiss-Prot
Match: ARD2_ARATH (1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 2 OS=Arabidopsis thaliana GN=ARD2 PE=2 SV=1)

HSP 1 Score: 230.335 bits (586), Expect = 2.358e-69
Identity = 96/147 (65.31%), Postives = 129/147 (87.76%), Query Frame = 3 
Query:   51 LEAWFMDESSQDQRLPHHRNPKQFVSLDQLAELGVLYWRLNPENYENDEELQKIREARGYSYMDLLDLCPEKVTNYEEKLKNFYTEHIHADEEIRYCLEGSGYFDVRDESDQWIRIWIKAGDLIVLPAGIYHRFTLDTSNYVKVMQI 491
            ++AW+MD+S +DQRLPHH++PK+FVSLD+LAELGVL WRL+ +NYE DE+L+KIRE+RGYSYMD  ++CPEK+ NYE K+K+F+ EH+H DEEIRYC+ G+GYFDVRD ++ WIR+ +K G +IVLPAGIYHRFT+D+ NY+K M++
Sbjct:   13 IQAWYMDDSEEDQRLPHHKDPKEFVSLDKLAELGVLSWRLDADNYETDEDLKKIRESRGYSYMDFCEVCPEKLPNYEVKVKSFFEEHLHTDEEIRYCVAGTGYFDVRDRNEAWIRVLVKKGGMIVLPAGIYHRFTVDSDNYIKAMRL 159

HSP 2 Score: 51.9878 bits (123), Expect = 2.358e-69
Identity = 21/30 (70.00%), Postives = 25/30 (83.33%), Query Frame = 2 
Query:  578 VQLMRLFVGEPVWTAYNRPQEDHPARKEYI 667
            ++ MRLFVGEPVWT YNRP +  PARKEY+
Sbjct:  154 IKAMRLFVGEPVWTPYNRPHDHLPARKEYV 183
[Close]
BLAST of EY736230 vs. ExPASy Swiss-Prot
Match: ARD4_ORYSJ (1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 4 OS=Oryza sativa subsp. japonica GN=ARD4 PE=2 SV=1)

HSP 1 Score: 206.068 bits (523), Expect = 4.376e-68
Identity = 90/149 (60.40%), Postives = 119/149 (79.87%), Query Frame = 3 
Query:   45 MALEAWFMDESSQDQRLPHHRNPKQFVSLDQLAELGVLYWRLNPENYENDEELQKIREARGYSYMDLLDLCPEKVTNYEEKLKNFYTEHIHADEEIRYCLEGSGYFDVRDESDQWIRIWIKAGDLIVLPAGIYHRFTLDTSNYVKVMQI 491
            MA   W + E+ +++      NP + + L +L E+GVLYW L+P+  E++EEL KIR  RGYSY DL ++CP+K+ NYEEKLK+FY EHIHADEEIRYCLEGSGYFDVRD+ D+WIRIWIK GD+I+LPAGIYHRF +D++NY+K+M++
Sbjct:    1 MAHLVWMLGENGEEKSF---ENPNELLPLSRLEEIGVLYWHLDPKKSESEEELTKIRRERGYSYFDLTEICPDKLENYEEKLKSFYCEHIHADEEIRYCLEGSGYFDVRDKDDKWIRIWIKEGDMIILPAGIYHRFIVDSNNYIKLMRL 146

HSP 2 Score: 72.0182 bits (175), Expect = 4.376e-68
Identity = 29/44 (65.91%), Postives = 35/44 (79.55%), Query Frame = 2 
Query:  578 VQLMRLFVGEPVWTAYNRPQEDHPARKEYIKGLTGQAGVPXEAH 709
            ++LMRLF+GEPVWTAYNRPQEDHP R+EY+K + G  G    AH
Sbjct:  141 IKLMRLFIGEPVWTAYNRPQEDHPVRQEYVKNVKGDTGFALAAH 184
[Close]
BLAST of EY736230 vs. ExPASy Swiss-Prot
Match: MTND_CHICK (1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase OS=Gallus gallus GN=ADI1 PE=2 SV=1)

HSP 1 Score: 199.134 bits (505), Expect = 3.313e-62
Identity = 89/147 (60.54%), Postives = 116/147 (78.91%), Query Frame = 3 
Query:   51 LEAWFMDESSQDQRLPHHRNPKQFVSLDQLAELGVLYWRLNPENYENDEELQKIREARGYSYMDLLDLCPEKVTNYEEKLKNFYTEHIHADEEIRYCLEGSGYFDVRDESDQWIRIWIKAGDLIVLPAGIYHRFTLDTSNYVKVMQI 491
            +EAW+MDES +DQR PH   P + VSL+QL  LGV Y RL+ +NYE D  L++IREA  YS+MD++ +  +K+ NYEEK+K FY EH+H D+EIRY L+GSGYFDVRD+ D+WIRI ++ GD+I LPAGIYHRFTLD +NYVK M++
Sbjct:    2 VEAWYMDESQEDQRAPHRLQPNRAVSLEQLRRLGVAYRRLDADNYETDPRLKEIREAENYSWMDIVTIHKDKLPNYEEKIKTFYEEHLHLDDEIRYILDGSGYFDVRDKDDKWIRISMEKGDMITLPAGIYHRFTLDENNYVKAMRL 148

HSP 2 Score: 59.3066 bits (142), Expect = 3.313e-62
Identity = 26/37 (70.27%), Postives = 31/37 (83.78%), Query Frame = 2 
Query:  578 VQLMRLFVGEPVWTAYNRPQEDHPARKEYIKGLTGQA 688
            V+ MRLFVGEPVWTAYNRP +D PARK+Y+K L  +A
Sbjct:  143 VKAMRLFVGEPVWTAYNRPADDFPARKQYMKFLAEEA 179
[Close]
BLAST of EY736230 vs. ExPASy Swiss-Prot
Match: MTND_RAT (1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase OS=Rattus norvegicus GN=Adi1 PE=2 SV=1)

HSP 1 Score: 203.371 bits (516), Expect = 4.706e-61
Identity = 88/147 (59.86%), Postives = 117/147 (79.59%), Query Frame = 3 
Query:   51 LEAWFMDESSQDQRLPHHRNPKQFVSLDQLAELGVLYWRLNPENYENDEELQKIREARGYSYMDLLDLCPEKVTNYEEKLKNFYTEHIHADEEIRYCLEGSGYFDVRDESDQWIRIWIKAGDLIVLPAGIYHRFTLDTSNYVKVMQI 491
            ++AW+MDES+ D R+PH   P + V L+QL  LGVLYW+L+ + YEND EL++IR+ R YS+MD++ +C + + NYEEK+K F+ EH+H DEEIRY LEGSGYFDVRD+ D+WIRI ++ GD+I LPAGIYHRFTLD  NYVK M++
Sbjct:    2 VQAWYMDESTADPRMPHRAQPDRPVGLEQLRTLGVLYWKLDADKYENDPELEQIRKTRNYSWMDIITICKDSLPNYEEKIKMFFEEHLHLDEEIRYILEGSGYFDVRDKEDKWIRISMEKGDMITLPAGIYHRFTLDEKNYVKAMRL 148

HSP 2 Score: 51.2174 bits (121), Expect = 4.706e-61
Identity = 23/37 (62.16%), Postives = 27/37 (72.97%), Query Frame = 2 
Query:  578 VQLMRLFVGEPVWTAYNRPQEDHPARKEYIKGLTGQA 688
            V+ MRLFVGEPVWT YNRP +   AR +Y+K L G A
Sbjct:  143 VKAMRLFVGEPVWTPYNRPADHFDARVQYVKFLEGTA 179
The following BLAST results are available for this feature:
BLAST of EY736230 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 26
Match NameE-valueIdentityDescription
ARD4_ARATH8.167e-8882.551,2-dihydroxy-3-keto-5-methylthiopentene dioxygena... [more]
ARD2_ORYSJ1.662e-7369.391,2-dihydroxy-3-keto-5-methylthiopentene dioxygena... [more]
ARD2_ORYSI1.662e-7369.391,2-dihydroxy-3-keto-5-methylthiopentene dioxygena... [more]
ARD1_ORYSJ1.518e-7168.711,2-dihydroxy-3-keto-5-methylthiopentene dioxygena... [more]
ARD1_ORYSI1.518e-7168.711,2-dihydroxy-3-keto-5-methylthiopentene dioxygena... [more]
ARD3_ARATH5.728e-7165.991,2-dihydroxy-3-keto-5-methylthiopentene dioxygena... [more]
ARD2_ARATH2.358e-6965.311,2-dihydroxy-3-keto-5-methylthiopentene dioxygena... [more]
ARD4_ORYSJ4.376e-6860.401,2-dihydroxy-3-keto-5-methylthiopentene dioxygena... [more]
MTND_CHICK3.313e-6260.541,2-dihydroxy-3-keto-5-methylthiopentene dioxygena... [more]
MTND_RAT4.706e-6159.861,2-dihydroxy-3-keto-5-methylthiopentene dioxygena... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionCS00-C3-705-013-E07-CT.F Sweet orange fruit, development stadium (6 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY736230 ID=EY736230; Name=EY736230; organism=Citrus sinensis; type=EST; length=827bp
CACGCGTCCGGAGTTCAGGCTGGAAGAGTGAGTTCTAGTTACCGATGGCT
CTCGAGGCGTGGTTTATGGATGAAAGTAGTCAAGACCAACGGCTCCCTCA
CCACCGCAACCCGAAGCAATTCGTATCGTTGGACCAATTGGCAGAATTGG
GAGTGCTGTACTGGCGCTTGAACCCGGAAAACTATGAGAACGATGAGGAA
TTGCAGAAGATCAGAGAAGCAAGAGGTTACAGTTACATGGATTTGCTTGA
TTTATGTCCGGAGAAAGTGACTAACTATGAGGAGAAGCTGAAGAACTTTT
ACACGGAGCACATCCATGCCGACGAAGAGATACGCTACTGTTTGGAAGGA
AGTGGATATTTTGATGTTAGAGACGAGAGTGATCAGTGGATTCGAATCTG
GATCAAAGCGGGTGATCTAATCGTTCTGCCAGCTGGAATCTACCATCGTT
TCACTCTTGACACTAGTAACTATGTTAAGGTAATGCAAATTTTAAGTTAT
GGTGTGCAAAGACAAAGTGATCAATGAATGCAATGGTTGGTTAATTAGAA
TTTAGAGCTATTGATTGACTCGTTGATGTGCAGTTGATGAGGTTGTTTGT
GGGAGAACCAGTGTGGACGGCATATAATCGGCCACAGGAGGATCATCCTG
CCAGAAAGGAGTACATCAAAGGTTTGACTGGGCAGGCAGGGGTGCCANTT
GAAGCTCATTAGAGTTTGAGAGTACTTTACCATTTACACTTGTGTATGTG
TATGTTAATGTAACTATATTTATATAGACTTCTGATAAACCACATTTAGC
TAAACCTTATGTCTATGCATTTGGTGC
back to top