EY735978

Overview
NameEY735978
Unique NameEY735978
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length837
Libraries
Library NameType
Sweet orange fruit, development stadium (6 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
EY735978_ssr472 microsatellite EY735978_ssr472:472..483. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig191 contig Csv1_Contig191:29..868. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of EY735978 vs. ExPASy Swiss-Prot
Match: CSK2A_MAIZE (Casein kinase II subunit alpha OS=Zea mays GN=ACK2 PE=1 SV=1)

HSP 1 Score: 246.514 bits (628), Expect = 2.545e-74
Identity = 118/164 (71.95%), Postives = 132/164 (80.49%), Query Frame = 1 
Query:  271 SKARVYSDINVVRPREYWDYESLTVQWGEQDDYEVVRKVGRGKYSEVFEGVHCTDNEXXXXXXXXXXXXXXXXXXXXXLQNLCGGPNIIKLLDIVRDQQSKTPSLIFEHVNNTDFKVLYPTLSDYDVRYYIYELLKALDYWHSQGICIEM*NPHNVMIDHEQKE 762
            SKARVY+D+NV+RP+EYWDYE+LTVQWGEQDDYEVVRKVGRGKYSEVFEG++  +NE                     LQNLCGGPNI+KLLDIVRDQ SKTPSLIFE+VNNTDFKVLYPTL+DYD+RYYIYELLKALDY HSQGI      PHNVMIDHE ++
Sbjct:    2 SKARVYADVNVLRPKEYWDYEALTVQWGEQDDYEVVRKVGRGKYSEVFEGINVNNNEKCIIKILKPVKKKKIKREIKILQNLCGGPNIVKLLDIVRDQHSKTPSLIFEYVNNTDFKVLYPTLTDYDIRYYIYELLKALDYCHSQGIMHRDVKPHNVMIDHELRK 165

HSP 2 Score: 52.373 bits (124), Expect = 2.545e-74
Identity = 23/26 (88.46%), Postives = 23/26 (88.46%), Query Frame = 2 
Query:  758 RKLRLIDWGSGEVYHPGKEYNVRVAS 835
            RKLRLIDWG  E YHPGKEYNVRVAS
Sbjct:  164 RKLRLIDWGLAEFYHPGKEYNVRVAS 189
[Close]
BLAST of EY735978 vs. ExPASy Swiss-Prot
Match: CSK22_ARATH (Casein kinase II subunit alpha-2 OS=Arabidopsis thaliana GN=CKA2 PE=2 SV=2)

HSP 1 Score: 241.506 bits (615), Expect = 8.038e-73
Identity = 114/164 (69.51%), Postives = 131/164 (79.88%), Query Frame = 1 
Query:  271 SKARVYSDINVVRPREYWDYESLTVQWGEQDDYEVVRKVGRGKYSEVFEGVHCTDNEXXXXXXXXXXXXXXXXXXXXXLQNLCGGPNIIKLLDIVRDQQSKTPSLIFEHVNNTDFKVLYPTLSDYDVRYYIYELLKALDYWHSQGICIEM*NPHNVMIDHEQKE 762
            SKARVY+D+NV+RP++YWDYESL VQWGEQDDYEVVRKVGRGKYSEVFEG++  +NE                     LQNLCGGPNI+KLLD+VRDQ SKTPSLIFE+VN+TDFKVLYPTL+DYD+RYYIYELLKALD+ HSQGI      PHNVMIDHE ++
Sbjct:    2 SKARVYTDVNVIRPKDYWDYESLNVQWGEQDDYEVVRKVGRGKYSEVFEGINMNNNEKCIIKILKPVKKKKIRREIKILQNLCGGPNIVKLLDVVRDQHSKTPSLIFEYVNSTDFKVLYPTLTDYDIRYYIYELLKALDFCHSQGIMHRDVKPHNVMIDHELRK 165

HSP 2 Score: 52.373 bits (124), Expect = 8.038e-73
Identity = 23/26 (88.46%), Postives = 23/26 (88.46%), Query Frame = 2 
Query:  758 RKLRLIDWGSGEVYHPGKEYNVRVAS 835
            RKLRLIDWG  E YHPGKEYNVRVAS
Sbjct:  164 RKLRLIDWGLAEFYHPGKEYNVRVAS 189
[Close]
BLAST of EY735978 vs. ExPASy Swiss-Prot
Match: CSK21_ARATH (Casein kinase II subunit alpha-1 OS=Arabidopsis thaliana GN=CKA1 PE=1 SV=2)

HSP 1 Score: 236.884 bits (603), Expect = 1.946e-71
Identity = 112/164 (68.29%), Postives = 129/164 (78.66%), Query Frame = 1 
Query:  271 SKARVYSDINVVRPREYWDYESLTVQWGEQDDYEVVRKVGRGKYSEVFEGVHCTDNEXXXXXXXXXXXXXXXXXXXXXLQNLCGGPNIIKLLDIVRDQQSKTPSLIFEHVNNTDFKVLYPTLSDYDVRYYIYELLKALDYWHSQGICIEM*NPHNVMIDHEQKE 762
            SKARVY+++NV+RP++YWDYESL VQWGEQDDYEVVRKVGRGKYSEVFEG++    E                     LQNLCGGPNI+KLLD+VRDQ SKTPSLIFE+VN+TDFKVLYPTL+DYD+RYYIYELLKALD+ HSQGI      PHNVMIDHE ++
Sbjct:    2 SKARVYTEVNVIRPKDYWDYESLIVQWGEQDDYEVVRKVGRGKYSEVFEGINVNSKEKCIIKILKPVKKKKIRREIKILQNLCGGPNIVKLLDVVRDQHSKTPSLIFEYVNSTDFKVLYPTLTDYDIRYYIYELLKALDFCHSQGIMHRDVKPHNVMIDHELRK 165

HSP 2 Score: 52.373 bits (124), Expect = 1.946e-71
Identity = 23/26 (88.46%), Postives = 23/26 (88.46%), Query Frame = 2 
Query:  758 RKLRLIDWGSGEVYHPGKEYNVRVAS 835
            RKLRLIDWG  E YHPGKEYNVRVAS
Sbjct:  164 RKLRLIDWGLAEFYHPGKEYNVRVAS 189
[Close]
BLAST of EY735978 vs. ExPASy Swiss-Prot
Match: CSK23_ARATH (Probable casein kinase II subunit alpha OS=Arabidopsis thaliana GN=At2g23080 PE=2 SV=1)

HSP 1 Score: 231.106 bits (588), Expect = 1.045e-69
Identity = 111/164 (67.68%), Postives = 125/164 (76.22%), Query Frame = 1 
Query:  271 SKARVYSDINVVRPREYWDYESLTVQWGEQDDYEVVRKVGRGKYSEVFEGVHCTDNEXXXXXXXXXXXXXXXXXXXXXLQNLCGGPNIIKLLDIVRDQQSKTPSLIFEHVNNTDFKVLYPTLSDYDVRYYIYELLKALDYWHSQGICIEM*NPHNVMIDHEQKE 762
            SKARVY+D+NVVRP+EYWDYESL VQWG QDDYEVVRKVGRGKYSEVFEG +   NE                     LQNLCGGPNI+KL DIVRD+ SKTPSL+FE VN+ DFKVLYPTL+DYD+RYYIYELLKALD+ HSQGI      PHNVMIDH+ ++
Sbjct:    2 SKARVYTDVNVVRPKEYWDYESLVVQWGHQDDYEVVRKVGRGKYSEVFEGKNVNTNERCVIKILKPVKKKKIKREIKILQNLCGGPNIVKLYDIVRDEHSKTPSLVFEFVNSVDFKVLYPTLTDYDIRYYIYELLKALDFCHSQGIMHRDVKPHNVMIDHQLRK 165

HSP 2 Score: 52.373 bits (124), Expect = 1.045e-69
Identity = 23/26 (88.46%), Postives = 23/26 (88.46%), Query Frame = 2 
Query:  758 RKLRLIDWGSGEVYHPGKEYNVRVAS 835
            RKLRLIDWG  E YHPGKEYNVRVAS
Sbjct:  164 RKLRLIDWGLAEFYHPGKEYNVRVAS 189
[Close]
BLAST of EY735978 vs. ExPASy Swiss-Prot
Match: CSK21_RAT (Casein kinase II subunit alpha OS=Rattus norvegicus GN=Csnk2a1 PE=1 SV=2)

HSP 1 Score: 211.46 bits (537), Expect = 2.286e-63
Identity = 99/165 (60.00%), Postives = 121/165 (73.33%), Query Frame = 1 
Query:  268 PSKARVYSDINVVRPREYWDYESLTVQWGEQDDYEVVRKVGRGKYSEVFEGVHCTDNEXXXXXXXXXXXXXXXXXXXXXLQNLCGGPNIIKLLDIVRDQQSKTPSLIFEHVNNTDFKVLYPTLSDYDVRYYIYELLKALDYWHSQGICIEM*NPHNVMIDHEQKE 762
            PS+ARVY+D+N  RPREYWDYES  V+WG QDDY++VRK+GRGKYSEVFE ++ T+NE                     L+NL GGPNII L DIV+D  S+TP+L+FEHVNNTDFK LY TL+DYD+R+Y+YE+LKALDY HS GI      PHNVMIDHE ++
Sbjct:    6 PSRARVYTDVNTHRPREYWDYESHVVEWGNQDDYQLVRKLGRGKYSEVFEAINITNNEKVVVKILKPVKKKKIKREIKILENLRGGPNIITLADIVKDPVSRTPALVFEHVNNTDFKQLYQTLTDYDIRFYMYEILKALDYCHSMGIMHRDVKPHNVMIDHEHRK 170

HSP 2 Score: 50.8322 bits (120), Expect = 2.286e-63
Identity = 22/26 (84.62%), Postives = 23/26 (88.46%), Query Frame = 2 
Query:  758 RKLRLIDWGSGEVYHPGKEYNVRVAS 835
            RKLRLIDWG  E YHPG+EYNVRVAS
Sbjct:  169 RKLRLIDWGLAEFYHPGQEYNVRVAS 194
[Close]
BLAST of EY735978 vs. ExPASy Swiss-Prot
Match: CSK21_MOUSE (Casein kinase II subunit alpha OS=Mus musculus GN=Csnk2a1 PE=2 SV=1)

HSP 1 Score: 211.46 bits (537), Expect = 2.286e-63
Identity = 99/165 (60.00%), Postives = 121/165 (73.33%), Query Frame = 1 
Query:  268 PSKARVYSDINVVRPREYWDYESLTVQWGEQDDYEVVRKVGRGKYSEVFEGVHCTDNEXXXXXXXXXXXXXXXXXXXXXLQNLCGGPNIIKLLDIVRDQQSKTPSLIFEHVNNTDFKVLYPTLSDYDVRYYIYELLKALDYWHSQGICIEM*NPHNVMIDHEQKE 762
            PS+ARVY+D+N  RPREYWDYES  V+WG QDDY++VRK+GRGKYSEVFE ++ T+NE                     L+NL GGPNII L DIV+D  S+TP+L+FEHVNNTDFK LY TL+DYD+R+Y+YE+LKALDY HS GI      PHNVMIDHE ++
Sbjct:    6 PSRARVYTDVNTHRPREYWDYESHVVEWGNQDDYQLVRKLGRGKYSEVFEAINITNNEKVVVKILKPVKKKKIKREIKILENLRGGPNIITLADIVKDPVSRTPALVFEHVNNTDFKQLYQTLTDYDIRFYMYEILKALDYCHSMGIMHRDVKPHNVMIDHEHRK 170

HSP 2 Score: 50.8322 bits (120), Expect = 2.286e-63
Identity = 22/26 (84.62%), Postives = 23/26 (88.46%), Query Frame = 2 
Query:  758 RKLRLIDWGSGEVYHPGKEYNVRVAS 835
            RKLRLIDWG  E YHPG+EYNVRVAS
Sbjct:  169 RKLRLIDWGLAEFYHPGQEYNVRVAS 194
[Close]
BLAST of EY735978 vs. ExPASy Swiss-Prot
Match: CSK21_HUMAN (Casein kinase II subunit alpha OS=Homo sapiens GN=CSNK2A1 PE=1 SV=1)

HSP 1 Score: 211.46 bits (537), Expect = 2.286e-63
Identity = 99/165 (60.00%), Postives = 121/165 (73.33%), Query Frame = 1 
Query:  268 PSKARVYSDINVVRPREYWDYESLTVQWGEQDDYEVVRKVGRGKYSEVFEGVHCTDNEXXXXXXXXXXXXXXXXXXXXXLQNLCGGPNIIKLLDIVRDQQSKTPSLIFEHVNNTDFKVLYPTLSDYDVRYYIYELLKALDYWHSQGICIEM*NPHNVMIDHEQKE 762
            PS+ARVY+D+N  RPREYWDYES  V+WG QDDY++VRK+GRGKYSEVFE ++ T+NE                     L+NL GGPNII L DIV+D  S+TP+L+FEHVNNTDFK LY TL+DYD+R+Y+YE+LKALDY HS GI      PHNVMIDHE ++
Sbjct:    6 PSRARVYTDVNTHRPREYWDYESHVVEWGNQDDYQLVRKLGRGKYSEVFEAINITNNEKVVVKILKPVKKKKIKREIKILENLRGGPNIITLADIVKDPVSRTPALVFEHVNNTDFKQLYQTLTDYDIRFYMYEILKALDYCHSMGIMHRDVKPHNVMIDHEHRK 170

HSP 2 Score: 50.8322 bits (120), Expect = 2.286e-63
Identity = 22/26 (84.62%), Postives = 23/26 (88.46%), Query Frame = 2 
Query:  758 RKLRLIDWGSGEVYHPGKEYNVRVAS 835
            RKLRLIDWG  E YHPG+EYNVRVAS
Sbjct:  169 RKLRLIDWGLAEFYHPGQEYNVRVAS 194
[Close]
BLAST of EY735978 vs. ExPASy Swiss-Prot
Match: CSK21_CHICK (Casein kinase II subunit alpha OS=Gallus gallus GN=CSNK2A1 PE=2 SV=1)

HSP 1 Score: 211.46 bits (537), Expect = 2.286e-63
Identity = 99/165 (60.00%), Postives = 121/165 (73.33%), Query Frame = 1 
Query:  268 PSKARVYSDINVVRPREYWDYESLTVQWGEQDDYEVVRKVGRGKYSEVFEGVHCTDNEXXXXXXXXXXXXXXXXXXXXXLQNLCGGPNIIKLLDIVRDQQSKTPSLIFEHVNNTDFKVLYPTLSDYDVRYYIYELLKALDYWHSQGICIEM*NPHNVMIDHEQKE 762
            PS+ARVY+D+N  RPREYWDYES  V+WG QDDY++VRK+GRGKYSEVFE ++ T+NE                     L+NL GGPNII L DIV+D  S+TP+L+FEHVNNTDFK LY TL+DYD+R+Y+YE+LKALDY HS GI      PHNVMIDHE ++
Sbjct:    6 PSRARVYTDVNTHRPREYWDYESHVVEWGNQDDYQLVRKLGRGKYSEVFEAINITNNEKVVVKILKPVKKKKIKREIKILENLRGGPNIITLADIVKDPVSRTPALVFEHVNNTDFKQLYQTLTDYDIRFYMYEILKALDYCHSMGIMHRDVKPHNVMIDHEHRK 170

HSP 2 Score: 50.8322 bits (120), Expect = 2.286e-63
Identity = 22/26 (84.62%), Postives = 23/26 (88.46%), Query Frame = 2 
Query:  758 RKLRLIDWGSGEVYHPGKEYNVRVAS 835
            RKLRLIDWG  E YHPG+EYNVRVAS
Sbjct:  169 RKLRLIDWGLAEFYHPGQEYNVRVAS 194
[Close]
BLAST of EY735978 vs. ExPASy Swiss-Prot
Match: CSK21_BOVIN (Casein kinase II subunit alpha OS=Bos taurus GN=CSNK2A1 PE=1 SV=1)

HSP 1 Score: 211.46 bits (537), Expect = 2.286e-63
Identity = 99/165 (60.00%), Postives = 121/165 (73.33%), Query Frame = 1 
Query:  268 PSKARVYSDINVVRPREYWDYESLTVQWGEQDDYEVVRKVGRGKYSEVFEGVHCTDNEXXXXXXXXXXXXXXXXXXXXXLQNLCGGPNIIKLLDIVRDQQSKTPSLIFEHVNNTDFKVLYPTLSDYDVRYYIYELLKALDYWHSQGICIEM*NPHNVMIDHEQKE 762
            PS+ARVY+D+N  RPREYWDYES  V+WG QDDY++VRK+GRGKYSEVFE ++ T+NE                     L+NL GGPNII L DIV+D  S+TP+L+FEHVNNTDFK LY TL+DYD+R+Y+YE+LKALDY HS GI      PHNVMIDHE ++
Sbjct:    6 PSRARVYTDVNTHRPREYWDYESHVVEWGNQDDYQLVRKLGRGKYSEVFEAINITNNEKVVVKILKPVKKKKIKREIKILENLRGGPNIITLADIVKDPVSRTPALVFEHVNNTDFKQLYQTLTDYDIRFYMYEILKALDYCHSMGIMHRDVKPHNVMIDHEHRK 170

HSP 2 Score: 50.8322 bits (120), Expect = 2.286e-63
Identity = 22/26 (84.62%), Postives = 23/26 (88.46%), Query Frame = 2 
Query:  758 RKLRLIDWGSGEVYHPGKEYNVRVAS 835
            RKLRLIDWG  E YHPG+EYNVRVAS
Sbjct:  169 RKLRLIDWGLAEFYHPGQEYNVRVAS 194
[Close]
BLAST of EY735978 vs. ExPASy Swiss-Prot
Match: CSK21_XENLA (Casein kinase II subunit alpha OS=Xenopus laevis GN=csnk2a1 PE=2 SV=2)

HSP 1 Score: 210.305 bits (534), Expect = 5.068e-63
Identity = 98/165 (59.39%), Postives = 121/165 (73.33%), Query Frame = 1 
Query:  268 PSKARVYSDINVVRPREYWDYESLTVQWGEQDDYEVVRKVGRGKYSEVFEGVHCTDNEXXXXXXXXXXXXXXXXXXXXXLQNLCGGPNIIKLLDIVRDQQSKTPSLIFEHVNNTDFKVLYPTLSDYDVRYYIYELLKALDYWHSQGICIEM*NPHNVMIDHEQKE 762
            PS+ARVY+D+N  RPR+YWDYES  V+WG QDDY++VRK+GRGKYSEVFE ++ T+NE                     L+NL GGPNII L DIV+D  S+TP+L+FEHVNNTDFK LY TL+DYD+R+Y+YE+LKALDY HS GI      PHNVMIDHE ++
Sbjct:    6 PSRARVYTDVNTHRPRDYWDYESHVVEWGNQDDYQLVRKLGRGKYSEVFEAINITNNEKVVVKILKPVKKKKIKREIKILENLRGGPNIITLADIVKDPVSRTPALVFEHVNNTDFKQLYQTLTDYDIRFYMYEILKALDYCHSMGIMHRDVKPHNVMIDHEHRK 170

HSP 2 Score: 50.8322 bits (120), Expect = 5.068e-63
Identity = 22/26 (84.62%), Postives = 23/26 (88.46%), Query Frame = 2 
Query:  758 RKLRLIDWGSGEVYHPGKEYNVRVAS 835
            RKLRLIDWG  E YHPG+EYNVRVAS
Sbjct:  169 RKLRLIDWGLAEFYHPGQEYNVRVAS 194
The following BLAST results are available for this feature:
BLAST of EY735978 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 27
Match NameE-valueIdentityDescription
CSK2A_MAIZE2.545e-7471.95Casein kinase II subunit alpha OS=Zea mays GN=ACK2... [more]
CSK22_ARATH8.038e-7369.51Casein kinase II subunit alpha-2 OS=Arabidopsis th... [more]
CSK21_ARATH1.946e-7168.29Casein kinase II subunit alpha-1 OS=Arabidopsis th... [more]
CSK23_ARATH1.045e-6967.68Probable casein kinase II subunit alpha OS=Arabido... [more]
CSK21_RAT2.286e-6360.00Casein kinase II subunit alpha OS=Rattus norvegicu... [more]
CSK21_MOUSE2.286e-6360.00Casein kinase II subunit alpha OS=Mus musculus GN=... [more]
CSK21_HUMAN2.286e-6360.00Casein kinase II subunit alpha OS=Homo sapiens GN=... [more]
CSK21_CHICK2.286e-6360.00Casein kinase II subunit alpha OS=Gallus gallus GN... [more]
CSK21_BOVIN2.286e-6360.00Casein kinase II subunit alpha OS=Bos taurus GN=CS... [more]
CSK21_XENLA5.068e-6359.39Casein kinase II subunit alpha OS=Xenopus laevis G... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-705-008-E03-CT.F Sweet orange fruit, development stadium (6 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY735978 ID=EY735978; Name=EY735978; organism=Citrus sinensis; type=EST; length=837bp
CACGCGTCCGAACACAACTCTCTTTTCATCTTTCTTTGCCATTTCATCAA
ACACATCATCGTCAAGAACAACATCGGTGTCAAAGATTCTCTTTTCTCCA
TTATCTCAATCTTCTCGTATATCACCCCTTTCGGCACTCTTCGCGGACTC
GCCTCCAGCGCGTCTCTATCCCACGCGCGACACCAACATCAACGGTTAAG
GCAGTTGGCCACGCCGCCGGACACGCTGGCTCAAAAGATCGGAAAATCGG
TTCGGCGCTCAGGCGCGCCCTCCAAAGCTAGGGTTTATTCCGATATTAAT
GTGGTTCGCCCCAGAGAGTACTGGGACTACGAGTCCCTCACCGTTCAGTG
GGGGGAGCAGGATGATTACGAGGTGGTGAGAAAGGTTGGGAGGGGGAAAT
ATAGTGAAGTGTTTGAAGGCGTGCACTGCACTGATAATGAAAAGTGTATT
ATTAAGATTCTTAAGCCTGTTAAGAAGAAGAAGATTAAGAGGGAGATAAA
AATACTTCAGAATCTTTGTGGTGGACCGAATATTATCAAGTTGCTTGATA
TTGTACGAGACCAGCAATCAAAGACCCCAAGTCTTATATTTGAACATGTG
AATAATACAGATTTTAAAGTGCTTTATCCAACACTCTCGGACTATGATGT
TCGGTATTACATCTATGAACTTCTAAAGGCATTGGATTACTGGCACTCAC
AAGGCATATGCATCGAGATGTGAAACCCCCATAATGTGATGATTGATCAT
GAACAAAAGGAAACTTCGTCTTATAGATTGGGGGTCTGGAGAGGTTTATC
ATCCTGGCAAAGAATATAATGTTCGTGTTGCTTCAAG
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