EY735745 | Citrus Genome Database

Overview


Name	EY735745
Unique Name	EY735745
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	867

Libraries


Library Name	Type
Sweet orange fruit, development stadium (6 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY735745 vs. ExPASy Swiss-Prot
Match: ISPH_CYAP4 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cyanothece sp. (strain PCC 7425 / ATCC 29141) GN=ispH PE=3 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 6.031e-24
Identity = 39/65 (60.00%), Postives = 52/65 (80.00%), Query Frame = 2

Query:  377 QERQDAVHKLVEEKIDLILVVGGWNSSNTSSLQVIAEDRGIPSYGIDSEKRIGPGNKIAYKLMVR 571
            QERQDA+ KLVE  +DL++V+GG+NSSNT+ LQ I+ +R IPSY IDS  RIGPGN++ +K + R
Sbjct:  293 QERQDAMFKLVEYPLDLMVVIGGFNSSNTTHLQEISIEREIPSYHIDSADRIGPGNRVEHKPLHR 357

HSP 2 Score: 50.0618 bits (118), Expect = 6.031e-24
Identity = 21/32 (65.62%), Postives = 26/32 (81.25%), Query Frame = 3

Query:  666 HGELVEKENWLPKGQITIGVTAGASTPDKVIS 761
            H  L  KENWLP+G I +G+T+GASTPDKV+S
Sbjct:  356 HRPLEIKENWLPEGPIVVGITSGASTPDKVVS 387

[Close]

BLAST of EY735745 vs. ExPASy Swiss-Prot
Match: ISPH_SYNP6 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) GN=ispH PE=3 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 2.251e-23
Identity = 39/61 (63.93%), Postives = 52/61 (85.25%), Query Frame = 2

Query:  377 QERQDAVHKLVEEKIDLILVVGGWNSSNTSSLQVIAEDRGIPSYGIDSEKRIGPGNKIAYK 559
            QERQDA+ +LVEE +DLI+V+GG+NSSNT+ LQ IA +R IPS+ ID+ +RIGPGN+I +K
Sbjct:  292 QERQDAMFQLVEEPLDLIVVIGGFNSSNTTHLQEIAIERQIPSFHIDAAERIGPGNRIEHK 352

HSP 2 Score: 46.595 bits (109), Expect = 2.251e-23
Identity = 19/31 (61.29%), Postives = 24/31 (77.42%), Query Frame = 3

Query:  666 HGELVEKENWLPKGQITIGVTAGASTPDKVI 758
            H +L   E WLP G +TIG+T+GASTPDKV+
Sbjct:  355 HTDLTTTEPWLPAGPLTIGITSGASTPDKVV 385

[Close]

BLAST of EY735745 vs. ExPASy Swiss-Prot
Match: ISPH_SYNE7 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Synechococcus elongatus (strain PCC 7942) GN=ispH PE=3 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 2.251e-23
Identity = 39/61 (63.93%), Postives = 52/61 (85.25%), Query Frame = 2

Query:  377 QERQDAVHKLVEEKIDLILVVGGWNSSNTSSLQVIAEDRGIPSYGIDSEKRIGPGNKIAYK 559
            QERQDA+ +LVEE +DLI+V+GG+NSSNT+ LQ IA +R IPS+ ID+ +RIGPGN+I +K
Sbjct:  292 QERQDAMFQLVEEPLDLIVVIGGFNSSNTTHLQEIAIERQIPSFHIDAAERIGPGNRIEHK 352

HSP 2 Score: 46.595 bits (109), Expect = 2.251e-23
Identity = 19/31 (61.29%), Postives = 24/31 (77.42%), Query Frame = 3

Query:  666 HGELVEKENWLPKGQITIGVTAGASTPDKVI 758
            H +L   E WLP G +TIG+T+GASTPDKV+
Sbjct:  355 HTDLTTTEPWLPAGPLTIGITSGASTPDKVV 385

[Close]

BLAST of EY735745 vs. ExPASy Swiss-Prot
Match: ISPH_ACAM1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Acaryochloris marina (strain MBIC 11017) GN=ispH PE=3 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 2.927e-23
Identity = 37/61 (60.66%), Postives = 51/61 (83.61%), Query Frame = 2

Query:  377 QERQDAVHKLVEEKIDLILVVGGWNSSNTSSLQVIAEDRGIPSYGIDSEKRIGPGNKIAYK 559
            QERQDA+ +LV+E +DL++V+GG+NSSNT+ LQ I+ D+ IPSY IDS  RIGPGN++ +K
Sbjct:  301 QERQDAIFQLVDEPLDLMVVIGGFNSSNTTHLQEISIDKNIPSYHIDSVDRIGPGNRVEHK 361

HSP 2 Score: 46.595 bits (109), Expect = 2.927e-23
Identity = 19/32 (59.38%), Postives = 25/32 (78.12%), Query Frame = 3

Query:  666 HGELVEKENWLPKGQITIGVTAGASTPDKVIS 761
            + +L   +NWLP G + IGVT+GASTPDKV+S
Sbjct:  364 NADLTVTDNWLPNGPLAIGVTSGASTPDKVVS 395

[Close]

BLAST of EY735745 vs. ExPASy Swiss-Prot
Match: ISPH_CYAA5 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cyanothece sp. (strain ATCC 51142) GN=ispH PE=3 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 4.958e-23
Identity = 38/61 (62.30%), Postives = 52/61 (85.25%), Query Frame = 2

Query:  377 QERQDAVHKLVEEKIDLILVVGGWNSSNTSSLQVIAEDRGIPSYGIDSEKRIGPGNKIAYK 559
            QERQDA+  LVEE++D+++V+GG+NSSNT+ LQ I+ ++GI SY IDS+KRI PGNKI +K
Sbjct:  292 QERQDAMLNLVEEEVDVMVVIGGFNSSNTTHLQEISIEKGIDSYHIDSDKRILPGNKIEHK 352

HSP 2 Score: 47.3654 bits (111), Expect = 4.958e-23
Identity = 19/29 (65.52%), Postives = 25/29 (86.21%), Query Frame = 3

Query:  672 ELVEKENWLPKGQITIGVTAGASTPDKVI 758
            ++  KENWLP G+I +GVT+GASTPDKV+
Sbjct:  357 DIETKENWLPDGKIIVGVTSGASTPDKVV 385

[Close]

BLAST of EY735745 vs. ExPASy Swiss-Prot
Match: ISPH_ANAVT (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Anabaena variabilis (strain ATCC 29413 / PCC 7937) GN=ispH PE=3 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 5.297e-22
Identity = 33/61 (54.10%), Postives = 49/61 (80.33%), Query Frame = 2

Query:  377 QERQDAVHKLVEEKIDLILVVGGWNSSNTSSLQVIAEDRGIPSYGIDSEKRIGPGNKIAYK 559
            QERQDA+ +LV+E +DL++V+GG+NSSNT+ LQ I+++RG+PSY ID  +RI   N I ++
Sbjct:  292 QERQDAMLELVQENLDLMIVIGGFNSSNTTQLQQISQERGLPSYHIDVVERIKSVNSIEHR 352

HSP 2 Score: 52.373 bits (124), Expect = 5.297e-22
Identity = 22/31 (70.97%), Postives = 27/31 (87.10%), Query Frame = 3

Query:  666 HGELVEKENWLPKGQITIGVTAGASTPDKVI 758
            +GELV  ENWLP G+I +GVT+GASTPDKV+
Sbjct:  355 NGELVTTENWLPAGKIVVGVTSGASTPDKVV 385

[Close]

BLAST of EY735745 vs. ExPASy Swiss-Prot
Match: ISPH_ANASP (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Anabaena sp. (strain PCC 7120) GN=ispH PE=3 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 6.891e-22
Identity = 33/61 (54.10%), Postives = 49/61 (80.33%), Query Frame = 2

Query:  377 QERQDAVHKLVEEKIDLILVVGGWNSSNTSSLQVIAEDRGIPSYGIDSEKRIGPGNKIAYK 559
            QERQDA+ +LV+E +DL++V+GG+NSSNT+ LQ I+++RG+PSY ID  +RI   N I ++
Sbjct:  292 QERQDAMLELVQENLDLMIVIGGFNSSNTTQLQQISQERGLPSYHIDVVERIKSINSIEHR 352

HSP 2 Score: 52.373 bits (124), Expect = 6.891e-22
Identity = 22/31 (70.97%), Postives = 27/31 (87.10%), Query Frame = 3

Query:  666 HGELVEKENWLPKGQITIGVTAGASTPDKVI 758
            +GELV  ENWLP G+I +GVT+GASTPDKV+
Sbjct:  355 NGELVTTENWLPAGKIVVGVTSGASTPDKVV 385

[Close]

BLAST of EY735745 vs. ExPASy Swiss-Prot
Match: ISPH_MICAN (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Microcystis aeruginosa (strain NIES-843) GN=ispH PE=3 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 3.340e-21
Identity = 37/61 (60.66%), Postives = 50/61 (81.97%), Query Frame = 2

Query:  377 QERQDAVHKLVEEKIDLILVVGGWNSSNTSSLQVIAEDRGIPSYGIDSEKRIGPGNKIAYK 559
            QERQDA+ +LV+E + L++V+GG+NSSNT+ LQ IA +R IPSY IDS +RI PGN+I +K
Sbjct:  292 QERQDAIFELVKEPLSLMVVIGGYNSSNTTHLQEIAIERAIPSYHIDSAERILPGNRIEHK 352

HSP 2 Score: 43.5134 bits (101), Expect = 3.340e-21
Identity = 17/29 (58.62%), Postives = 25/29 (86.21%), Query Frame = 3

Query:  672 ELVEKENWLPKGQITIGVTAGASTPDKVI 758
            +L+  +NWL +G+I +GVT+GASTPDKV+
Sbjct:  357 DLIITDNWLNEGKIVVGVTSGASTPDKVV 385

[Close]

BLAST of EY735745 vs. ExPASy Swiss-Prot
Match: ISPH_NOSP7 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Nostoc punctiforme (strain ATCC 29133 / PCC 73102) GN=ispH PE=3 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 1.618e-20
Identity = 33/61 (54.10%), Postives = 48/61 (78.69%), Query Frame = 2

Query:  377 QERQDAVHKLVEEKIDLILVVGGWNSSNTSSLQVIAEDRGIPSYGIDSEKRIGPGNKIAYK 559
            QERQDA+ +LVE  ++L++V+GG+NSSNT+ LQ IA +R IPSY ID+ +RI  G+ I ++
Sbjct:  292 QERQDAMLELVEHNLNLMVVIGGFNSSNTTQLQQIAFERRIPSYHIDTVERIKSGDSIEHR 352

HSP 2 Score: 50.8322 bits (120), Expect = 1.618e-20
Identity = 19/31 (61.29%), Postives = 27/31 (87.10%), Query Frame = 3

Query:  666 HGELVEKENWLPKGQITIGVTAGASTPDKVI 758
            +G+L+  ENWLP G+I +G+T+GASTPDKV+
Sbjct:  355 NGQLITTENWLPDGEIVVGITSGASTPDKVV 385

[Close]

BLAST of EY735745 vs. ExPASy Swiss-Prot
Match: ISPH_SYNR3 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Synechococcus sp. (strain RCC307) GN=ispH PE=3 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 7.834e-20
Identity = 37/61 (60.66%), Postives = 49/61 (80.33%), Query Frame = 2

Query:  377 QERQDAVHKLVEEKIDLILVVGGWNSSNTSSLQVIAEDRGIPSYGIDSEKRIGPGNKIAYK 559
            QERQDA+  LV+E +DL++V+GG+NSSNT+ LQ IA  RGIPS  ID+ +RIGPGN + +K
Sbjct:  292 QERQDAMFSLVDEPLDLMVVIGGYNSSNTTHLQEIAVSRGIPSVHIDAPERIGPGNAVEHK 352

HSP 2 Score: 37.3502 bits (85), Expect = 7.834e-20
Identity = 15/29 (51.72%), Postives = 23/29 (79.31%), Query Frame = 3

Query:  672 ELVEKENWLPKGQITIGVTAGASTPDKVI 758
            +L   E +LP+G + IG+T+GASTPD+V+
Sbjct:  357 DLERLEPFLPEGDLRIGITSGASTPDRVV 385

The following BLAST results are available for this feature:

BLAST of EY735745 vs. ExPASy Swiss-Prot

BLAST of EY735745 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 28


Match Name	E-value	Identity	Description
ISPH_CYAP4	6.031e-24	60.00	4-hydroxy-3-methylbut-2-enyl diphosphate reductase...	[more]
ISPH_SYNP6	2.251e-23	63.93	4-hydroxy-3-methylbut-2-enyl diphosphate reductase...	[more]
ISPH_SYNE7	2.251e-23	63.93	4-hydroxy-3-methylbut-2-enyl diphosphate reductase...	[more]
ISPH_ACAM1	2.927e-23	60.66	4-hydroxy-3-methylbut-2-enyl diphosphate reductase...	[more]
ISPH_CYAA5	4.958e-23	62.30	4-hydroxy-3-methylbut-2-enyl diphosphate reductase...	[more]
ISPH_ANAVT	5.297e-22	54.10	4-hydroxy-3-methylbut-2-enyl diphosphate reductase...	[more]
ISPH_ANASP	6.891e-22	54.10	4-hydroxy-3-methylbut-2-enyl diphosphate reductase...	[more]
ISPH_MICAN	3.340e-21	60.66	4-hydroxy-3-methylbut-2-enyl diphosphate reductase...	[more]
ISPH_NOSP7	1.618e-20	54.10	4-hydroxy-3-methylbut-2-enyl diphosphate reductase...	[more]
ISPH_SYNR3	7.834e-20	60.66	4-hydroxy-3-methylbut-2-enyl diphosphate reductase...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C3-705-004-D07-CT.F Sweet orange fruit, development stadium (6 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY735745 ID=EY735745; Name=EY735745; organism=Citrus sinensis; type=EST; length=867bp
CACGCGTCCGGGAAAATTAGTGGAGAAGACCATGATGCGTAAATTTGGAG
TTGAAAATGTCAATGAACACTTCATAAGCTTCAACACAATTTGTCATGGT
GCACAAGTAATTTCTTCTCCCTCATGGTTTTATCCTTTCAGATTTTTATA
ATTTTGTGAGTCAGAGAACTTCCTGTCCACTGCATGTCTTTTAACGCACT
TAATAAAACCCAGAATGTTCAACTTCGGTATGCTAAACCTAACAACATAG
TTGACAATTTGTTTTCAATGACCGACTGCTCCGCCAGCAATACTCAGCCG
CTAGTTAATCTGCAAGTTGTGTACCATTCTGTTGTGTTCAGTAAATTTAA
GCTAATGATTTTGGTCTCAATTTTTGCAGGAGCGACAAGATGCAGTGCAT
AAGTTGGTTGAGGAAAAGATCGATCTTATTTTGGTTGTTGGTGGGTGGAA
CTCGAGCAATACATCAAGCCTTCAAGTGATTGCAGAAGACCGTGGAATTC
CCTCATACGGGATCGACAGTGAGAAGAGAATAGGTCCCGGAAACAAAATA
GCGTATAAATTGATGGTACGTGTGAACATATTTGAATTTTACTTGCATCA
ACAGAGTAATTTTTTGGTTTCAAAATTTCGTTATTAATTATCTGTGTTTG
TTATCTTGTATGTANCATGGCGAGCTGGTGGAGAAAGAAAATTGGCTGCC
GAAAGGACAGATCACAATCGGGGTGACAGCCGGTGCCTCCACCCCAGATA
AAGTAATTTCTTACGCAGGAAATTTCTAAATAGATTCTTGCTTACCTTGA
CCTTATTTTTTCATGTTTGTATTTCTAATTTACCATGCCACAGCTAATTA
CTCATTTTCGGCTGCTG