EY735725 | Citrus Genome Database

Overview


Name	EY735725
Unique Name	EY735725
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	654

Libraries


Library Name	Type
Sweet orange fruit, development stadium (6 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY735725 vs. ExPASy Swiss-Prot
Match: HSCB_PSEFS (Co-chaperone protein hscB homolog OS=Pseudomonas fluorescens (strain SBW25) GN=hscB PE=3 SV=1)

HSP 1 Score: 147.517 bits (371), Expect = 6.157e-54
Identity = 72/77 (93.51%), Postives = 76/77 (98.70%), Query Frame = 3

Query:   18 VGTPCHFALFELQPSFRLDLEQLATRYRELARGVHPDRFADASEREQRLALEQSASLHEAYQTLKNPPKRARXLLAM 248
            +GTPCHFALFELQP+FRLDLEQLATRYRELARGVHPDRFADASEREQRLALE+SASL+EAYQTLKNPPKRAR LLAM
Sbjct:    1 MGTPCHFALFELQPNFRLDLEQLATRYRELARGVHPDRFADASEREQRLALEKSASLNEAYQTLKNPPKRARYLLAM 77

HSP 2 Score: 85.5001 bits (210), Expect = 6.157e-54
Identity = 51/99 (51.52%), Postives = 60/99 (60.61%), Query Frame = 1

Query:  253 GRELPLEVTVXEPDFLMQQNAWRXXXXXXXXXXXXXGVAVFXRRLKIGPV*TQRKLPSLFGI---NPPHLSRAKRLMRRMQFLGQLTYKVRQLEERLDD 540
            G E+P+EVTV +PDFLMQQ  WR             GVAVF RRLK      Q +L   F     +     +A+RLMRRMQFL +LTY+VRQLEERLDD
Sbjct:   79 GGEVPIEVTVHDPDFLMQQMQWREELEDLQDEADVAGVAVFKRRLKT----AQDELNESFAACWNDAAQREQAERLMRRMQFLDKLTYEVRQLEERLDD 173

[Close]

BLAST of EY735725 vs. ExPASy Swiss-Prot
Match: HSCB_PSESM (Co-chaperone protein hscB homolog OS=Pseudomonas syringae pv. tomato GN=hscB PE=3 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 1.867e-46
Identity = 65/77 (84.42%), Postives = 73/77 (94.81%), Query Frame = 3

Query:   18 VGTPCHFALFELQPSFRLDLEQLATRYRELARGVHPDRFADASEREQRLALEQSASLHEAYQTLKNPPKRARXLLAM 248
            +G+PCHFALFEL+P F+LDL+QLATRYRELAR VHPDRFADA EREQRLALE+SASL+EAYQTLK+PPKRAR LLAM
Sbjct:    1 MGSPCHFALFELKPEFQLDLDQLATRYRELARNVHPDRFADAPEREQRLALERSASLNEAYQTLKSPPKRARYLLAM 77

HSP 2 Score: 72.7886 bits (177), Expect = 1.867e-46
Identity = 41/96 (42.71%), Postives = 57/96 (59.38%), Query Frame = 1

Query:  253 GRELPLEVTVXEPDFLMQQNAWRXXXXXXXXXXXXXGVAVFXRRLKIGPV*TQRKLPSLFGINPPHLSRAKRLMRRMQFLGQLTYKVRQLEERLDD 540
            G E+PLEVTV +P+FL+QQ   R             GVA F R+LK+      +   + +  +      A++LMRRMQFL +L+++VRQLEERLDD
Sbjct:   79 GNEVPLEVTVHDPEFLLQQMQLREDLEDLQDEADLAGVATFKRQLKVAQDELNQSFAACWN-DAAQREHAEKLMRRMQFLDKLSHEVRQLEERLDD 173

[Close]

BLAST of EY735725 vs. ExPASy Swiss-Prot
Match: HSCB_PSEMY (Co-chaperone protein hscB homolog OS=Pseudomonas mendocina (strain ymp) GN=hscB PE=3 SV=1)

HSP 1 Score: 127.102 bits (318), Expect = 3.440e-45
Identity = 62/77 (80.52%), Postives = 68/77 (88.31%), Query Frame = 3

Query:   18 VGTPCHFALFELQPSFRLDLEQLATRYRELARGVHPDRFADASEREQRLALEQSASLHEAYQTLKNPPKRARXLLAM 248
            +G PCHFALF+LQPSFRLDLEQLA RYRELAR VHPDRFADA EREQRLALE+SA L+EAYQ LK P +RAR LLA+
Sbjct:    1 MGNPCHFALFDLQPSFRLDLEQLAARYRELARQVHPDRFADAGEREQRLALERSACLNEAYQVLKTPSQRARYLLAL 77

HSP 2 Score: 76.6406 bits (187), Expect = 3.440e-45
Identity = 48/99 (48.48%), Postives = 57/99 (57.58%), Query Frame = 1

Query:  253 GRELPLEVTVXEPDFLMQQNAWRXXXXXXXXXXXXXGVAVFXRRLKIGPV*TQRKLPSLFGI---NPPHLSRAKRLMRRMQFLGQLTYKVRQLEERLDD 540
            G ELPLEVTV +PDFL+QQ   R             GVA F RRLK      Q +L   F     +      A+RLMRRMQFL +L+++VRQLEERLDD
Sbjct:   79 GPELPLEVTVQDPDFLLQQMQLREELEELQDDADLAGVAAFKRRLKTA----QEQLNDSFAACWDDDTRREEAERLMRRMQFLDKLSHEVRQLEERLDD 173

[Close]

BLAST of EY735725 vs. ExPASy Swiss-Prot
Match: HSCB_PSEU5 (Co-chaperone protein hscB homolog OS=Pseudomonas stutzeri (strain A1501) GN=hscB PE=3 SV=1)

HSP 1 Score: 120.168 bits (300), Expect = 3.362e-42
Identity = 58/77 (75.32%), Postives = 67/77 (87.01%), Query Frame = 3

Query:   18 VGTPCHFALFELQPSFRLDLEQLATRYRELARGVHPDRFADASEREQRLALEQSASLHEAYQTLKNPPKRARXLLAM 248
            +GTPCHFALF+L+P F LDL QLA RYRELAR VHPDRFADA E EQR ALE+SA+L+EAYQTLK+P +RAR LLA+
Sbjct:    1 MGTPCHFALFDLKPDFELDLAQLADRYRELARQVHPDRFADAGESEQRQALERSANLNEAYQTLKSPSRRARYLLAL 77

HSP 2 Score: 73.559 bits (179), Expect = 3.362e-42
Identity = 47/99 (47.47%), Postives = 56/99 (56.57%), Query Frame = 1

Query:  253 GRELPLEVTVXEPDFLMQQNAWRXXXXXXXXXXXXXGVAVFXRRLKIGPV*TQRKLPSLFGI---NPPHLSRAKRLMRRMQFLGQLTYKVRQLEERLDD 540
            G ++PLE TV +P FLMQQ  WR             GVA F  RLK      Q+ L   FG    +P     A+RL+RRMQFL +LT +VRQLEERLDD
Sbjct:   79 GHDVPLEATVQDPAFLMQQMHWREELEELHEQADLDGVAAFKSRLKQA----QQGLNDDFGQIWRDPARRVDAERLVRRMQFLDKLTQEVRQLEERLDD 173

[Close]

BLAST of EY735725 vs. ExPASy Swiss-Prot
Match: HSCB_PSEAE (Co-chaperone protein hscB homolog OS=Pseudomonas aeruginosa GN=hscB PE=3 SV=1)

HSP 1 Score: 111.694 bits (278), Expect = 8.718e-40
Identity = 52/77 (67.53%), Postives = 64/77 (83.12%), Query Frame = 3

Query:   18 VGTPCHFALFELQPSFRLDLEQLATRYRELARGVHPDRFADASEREQRLALEQSASLHEAYQTLKNPPKRARXLLAM 248
            +G PCHFA F+LQP+F +DL++L  RYREL R VHPDRFADA EREQRLALE++A L+EAYQTLK+ P+RA  LL +
Sbjct:    1 MGKPCHFAQFDLQPAFLVDLDELGQRYRELVRSVHPDRFADAPEREQRLALERAAQLNEAYQTLKSAPRRALYLLTL 77

HSP 2 Score: 73.9442 bits (180), Expect = 8.718e-40
Identity = 43/96 (44.79%), Postives = 55/96 (57.29%), Query Frame = 1

Query:  253 GRELPLEVTVXEPDFLMQQNAWRXXXXXXXXXXXXXGVAVFXRRLKIGPV*TQRKLPSLFGINPPHLSRAKRLMRRMQFLGQLTYKVRQLEERLDD 540
            G ELPLE TV +P+FL+QQ   R             GVA F RRLK      +R+  + +  +      A+RL+RRMQFL +L  +VRQLEERLDD
Sbjct:   79 GHELPLEATVQDPEFLLQQMQLREELEELQDSADLAGVATFKRRLKAAQAELEREFAACWD-DAQRREEAERLVRRMQFLDKLAQEVRQLEERLDD 173

[Close]

BLAST of EY735725 vs. ExPASy Swiss-Prot
Match: HSCB_PSEAB (Co-chaperone protein hscB homolog OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=hscB PE=3 SV=1)

HSP 1 Score: 111.694 bits (278), Expect = 8.718e-40
Identity = 52/77 (67.53%), Postives = 64/77 (83.12%), Query Frame = 3

Query:   18 VGTPCHFALFELQPSFRLDLEQLATRYRELARGVHPDRFADASEREQRLALEQSASLHEAYQTLKNPPKRARXLLAM 248
            +G PCHFA F+LQP+F +DL++L  RYREL R VHPDRFADA EREQRLALE++A L+EAYQTLK+ P+RA  LL +
Sbjct:    1 MGKPCHFAQFDLQPAFLVDLDELGQRYRELVRSVHPDRFADAPEREQRLALERAAQLNEAYQTLKSAPRRALYLLTL 77

HSP 2 Score: 73.9442 bits (180), Expect = 8.718e-40
Identity = 43/96 (44.79%), Postives = 55/96 (57.29%), Query Frame = 1

Query:  253 GRELPLEVTVXEPDFLMQQNAWRXXXXXXXXXXXXXGVAVFXRRLKIGPV*TQRKLPSLFGINPPHLSRAKRLMRRMQFLGQLTYKVRQLEERLDD 540
            G ELPLE TV +P+FL+QQ   R             GVA F RRLK      +R+  + +  +      A+RL+RRMQFL +L  +VRQLEERLDD
Sbjct:   79 GHELPLEATVQDPEFLLQQMQLREELEELQDSADLAGVATFKRRLKAAQAELEREFAACWD-DAQRREEAERLVRRMQFLDKLAQEVRQLEERLDD 173

[Close]

BLAST of EY735725 vs. ExPASy Swiss-Prot
Match: HSCB_PSEA8 (Co-chaperone protein hscB homolog OS=Pseudomonas aeruginosa (strain LESB58) GN=hscB PE=3 SV=1)

HSP 1 Score: 111.694 bits (278), Expect = 8.718e-40
Identity = 52/77 (67.53%), Postives = 64/77 (83.12%), Query Frame = 3

Query:   18 VGTPCHFALFELQPSFRLDLEQLATRYRELARGVHPDRFADASEREQRLALEQSASLHEAYQTLKNPPKRARXLLAM 248
            +G PCHFA F+LQP+F +DL++L  RYREL R VHPDRFADA EREQRLALE++A L+EAYQTLK+ P+RA  LL +
Sbjct:    1 MGKPCHFAQFDLQPAFLVDLDELGQRYRELVRSVHPDRFADAPEREQRLALERAAQLNEAYQTLKSAPRRALYLLTL 77

HSP 2 Score: 73.9442 bits (180), Expect = 8.718e-40
Identity = 43/96 (44.79%), Postives = 55/96 (57.29%), Query Frame = 1

Query:  253 GRELPLEVTVXEPDFLMQQNAWRXXXXXXXXXXXXXGVAVFXRRLKIGPV*TQRKLPSLFGINPPHLSRAKRLMRRMQFLGQLTYKVRQLEERLDD 540
            G ELPLE TV +P+FL+QQ   R             GVA F RRLK      +R+  + +  +      A+RL+RRMQFL +L  +VRQLEERLDD
Sbjct:   79 GHELPLEATVQDPEFLLQQMQLREELEELQDSADLAGVATFKRRLKAAQAELEREFAACWD-DAQRREEAERLVRRMQFLDKLAQEVRQLEERLDD 173

[Close]

BLAST of EY735725 vs. ExPASy Swiss-Prot
Match: HSCB_PSEA7 (Co-chaperone protein hscB homolog OS=Pseudomonas aeruginosa (strain PA7) GN=hscB PE=3 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 1.229e-38
Identity = 51/77 (66.23%), Postives = 64/77 (83.12%), Query Frame = 3

Query:   18 VGTPCHFALFELQPSFRLDLEQLATRYRELARGVHPDRFADASEREQRLALEQSASLHEAYQTLKNPPKRARXLLAM 248
            +G PCHFA F+LQP+F +DL++L  RYREL R VHPDRFADA EREQRLALE++A L++AYQTLK+ P+RA  LL +
Sbjct:    1 MGKPCHFAQFDLQPTFLVDLDELGRRYRELVRSVHPDRFADAPEREQRLALERAAQLNDAYQTLKSAPRRALYLLTL 77

HSP 2 Score: 71.2478 bits (173), Expect = 1.229e-38
Identity = 42/96 (43.75%), Postives = 55/96 (57.29%), Query Frame = 1

Query:  253 GRELPLEVTVXEPDFLMQQNAWRXXXXXXXXXXXXXGVAVFXRRLKIGPV*TQRKLPSLFGINPPHLSRAKRLMRRMQFLGQLTYKVRQLEERLDD 540
            GRELPLE TV +P+FL+QQ   R             GVA F R+LK      + +  + +  +      A+RL+RRMQFL +L  +VRQLEERLDD
Sbjct:   79 GRELPLEATVQDPEFLLQQMQLREELEELQDSADLAGVATFKRQLKAAQAELEGEFAACWD-DARRREEAERLVRRMQFLDKLAQEVRQLEERLDD 173

[Close]

BLAST of EY735725 vs. ExPASy Swiss-Prot
Match: HSCB_AZOVD (Co-chaperone protein hscB homolog OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=hscB PE=3 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 2.632e-35
Identity = 52/77 (67.53%), Postives = 64/77 (83.12%), Query Frame = 3

Query:   18 VGTPCHFALFELQPSFRLDLEQLATRYRELARGVHPDRFADASEREQRLALEQSASLHEAYQTLKNPPKRARXLLAM 248
            +G   HFALF+L+P FRLD ++LA RYREL R VHPDRFA A EREQRLALE++A L+EAYQTLK+P +RAR LL++
Sbjct:    1 MGNASHFALFDLEPDFRLDQDRLAVRYRELVRRVHPDRFAGAPEREQRLALEEAARLNEAYQTLKSPSQRARYLLSL 77

HSP 2 Score: 62.3882 bits (150), Expect = 2.632e-35
Identity = 45/99 (45.45%), Postives = 51/99 (51.52%), Query Frame = 1

Query:  253 GRELPLEVTVXEPDFLMQQNAWRXXXXXXXXXXXXXGVAVFXRRLKIGPV*TQRKLPSLFGI---NPPHLSRAKRLMRRMQFLGQLTYKVRQLEERLDD 540
            G EL  E TV +P FLMQQ   R             GVA F RRL    V  Q +L   F     +P     A+RL RRMQFL +L  +VRQLEERLDD
Sbjct:   79 GEELSQETTVQDPAFLMQQMELREELQELQDSADLAGVARFKRRL----VQDQEQLNEGFAACWADPRRRDEAERLARRMQFLDKLFAEVRQLEERLDD 173

[Close]

BLAST of EY735725 vs. ExPASy Swiss-Prot
Match: HSCB_AZOVI (Co-chaperone protein hscB homolog OS=Azotobacter vinelandii GN=hscB PE=3 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 9.877e-35
Identity = 51/72 (70.83%), Postives = 62/72 (86.11%), Query Frame = 3

Query:   33 HFALFELQPSFRLDLEQLATRYRELARGVHPDRFADASEREQRLALEQSASLHEAYQTLKNPPKRARXLLAM 248
            HFALF+L+P FRLD ++LA RYREL R VHPDRFA A EREQRLALE++A L+EAYQTLK+P +RAR LL++
Sbjct:    4 HFALFDLEPDFRLDQDRLAVRYRELVRRVHPDRFAGAPEREQRLALEEAARLNEAYQTLKSPSQRARYLLSL 75

HSP 2 Score: 62.3882 bits (150), Expect = 9.877e-35
Identity = 45/99 (45.45%), Postives = 51/99 (51.52%), Query Frame = 1

Query:  253 GRELPLEVTVXEPDFLMQQNAWRXXXXXXXXXXXXXGVAVFXRRLKIGPV*TQRKLPSLFGI---NPPHLSRAKRLMRRMQFLGQLTYKVRQLEERLDD 540
            G EL  E TV +P FLMQQ   R             GVA F RRL    V  Q +L   F     +P     A+RL RRMQFL +L  +VRQLEERLDD
Sbjct:   77 GEELSQETTVQDPAFLMQQMELREELQELQDSADLAGVARFKRRL----VQDQEQLNEGFAACWADPRRRDEAERLARRMQFLDKLFAEVRQLEERLDD 171

The following BLAST results are available for this feature:

BLAST of EY735725 vs. ExPASy Swiss-Prot

BLAST of EY735725 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 118


Match Name	E-value	Identity	Description
HSCB_PSEFS	6.157e-54	93.51	Co-chaperone protein hscB homolog OS=Pseudomonas f...	[more]
HSCB_PSESM	1.867e-46	84.42	Co-chaperone protein hscB homolog OS=Pseudomonas s...	[more]
HSCB_PSEMY	3.440e-45	80.52	Co-chaperone protein hscB homolog OS=Pseudomonas m...	[more]
HSCB_PSEU5	3.362e-42	75.32	Co-chaperone protein hscB homolog OS=Pseudomonas s...	[more]
HSCB_PSEAE	8.718e-40	67.53	Co-chaperone protein hscB homolog OS=Pseudomonas a...	[more]
HSCB_PSEAB	8.718e-40	67.53	Co-chaperone protein hscB homolog OS=Pseudomonas a...	[more]
HSCB_PSEA8	8.718e-40	67.53	Co-chaperone protein hscB homolog OS=Pseudomonas a...	[more]
HSCB_PSEA7	1.229e-38	66.23	Co-chaperone protein hscB homolog OS=Pseudomonas a...	[more]
HSCB_AZOVD	2.632e-35	67.53	Co-chaperone protein hscB homolog OS=Azotobacter v...	[more]
HSCB_AZOVI	9.877e-35	70.83	Co-chaperone protein hscB homolog OS=Azotobacter v...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C3-705-004-B09-CT.F Sweet orange fruit, development stadium (6 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY735725 ID=EY735725; Name=EY735725; organism=Citrus sinensis; type=EST; length=654bp
AACATCTGAGGCTGATTGTGGGTACTCCTTGTCATTTCGCTTTATTCGAG
CTGCAGCCGAGTTTTCGACTGGACCTTGAACAGCTTGCCACGCGCTACCG
TGAACTGGCGCGTGGGGTGCATCCGGACCGCTTTGCTGACGCTTCCGAGC
GTGAGCAACGCTTGGCGCTCGAGCAATCAGCCAGCCTCCACGAAGCCTAT
CAGACGCTCAAGAACCCTCCCAAACGCGCGCGCTNACTGCTCGCCATGGG
GCGGTCGCGAGCTGCCGCTTGAAGTCACGGTCCANGAACCTGATTTCCTG
ATGCAGCAGAATGCATGGCGCGAGGAGCTCGAAGAACTGCAGGACGAGGC
CGATCTGGCCGGAGTCGCGGTCTTCAANCGCCGTCTTAAAATTGGCCCAG
TATAAACTCAACGAAAACTTCCCAGCCTGTTTGGGATTAACCCGCCCCAC
TTAAGCAGGGCCAAACGCCTGATGCGGCGCATGCAGTTCCTCGGACAGCT
CACCTACAAAGTGCGCCAGTTAGAAGAGCGCCTCGACGATTAACCCAGTG
CCGCCACGTTGTACGCCTGATATAGAGAAAGGGCTCGATAACAGCGGGGC
CCCTGCTGGATGATCGCACCAACCGGGGCAAGTCCTCGACCCCACCCACG
TCCT