EY735627

Overview
NameEY735627
Unique NameEY735627
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length846
Libraries
Library NameType
Sweet orange fruit, development stadium (6 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY735627 vs. ExPASy Swiss-Prot
Match: RPN1_PIG (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 OS=Sus scrofa GN=RPN1 PE=1 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 2.262e-19
Identity = 39/106 (36.79%), Postives = 60/106 (56.60%), Query Frame = 2 
Query:   83 YYTIVLANPLNTGETATLEILYILTHSLQPFPVEISQS*SQLVYYHDNALILSPYHIKQQTTFIKIPSTKVESFTRVEPTDRAGNELKYGPYEDRPPYSYSPLLVH 400
            ++T+ L   L+ G   ++ +  + TH LQP+P +I+QS  Q V +  N    SPY  K Q+  +K+ S  VES+T++    R+ + L YGP+ D PPYS     VH
Sbjct:  113 FFTVTLPVALDPGAKISVTVETVYTHVLQPYPTQITQSEKQFVVFEGNHYFYSPYPTKSQSMRVKLASRNVESYTKLGNPTRSEDLLDYGPFRDVPPYSQDTFKVH 218

HSP 2 Score: 36.1946 bits (82), Expect = 2.262e-19
Identity = 14/45 (31.11%), Postives = 27/45 (60.00%), Query Frame = 3 
Query:  405 ENNNPFAVVEELVREVDISRWGHLAITEQATCLMLVRETKACVRG 539
            ENN+PF  +  + R +++S WG++A+ E       ++ T A ++G
Sbjct:  220 ENNSPFLTITSMTRVIEVSHWGNIAVEENVD----LKHTGAVLKG 260
[Close]
BLAST of EY735627 vs. ExPASy Swiss-Prot
Match: RPN1_MOUSE (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 OS=Mus musculus GN=Rpn1 PE=2 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 1.094e-18
Identity = 38/107 (35.51%), Postives = 60/107 (56.07%), Query Frame = 2 
Query:   83 YYTIVLANPLNTGETATLEILYILTHSLQPFPVEISQS*SQLVYYHDNALILSPYHIKQQTTFIKIPSTKVESFTRVEPTDRAGNELKYGPYEDRPPYSYSPLLVHF 403
            ++T+ L   L+ G   ++ +  + TH L P+P +I+QS  Q V +  N    SPY  K QT  +K+ S  VES+T++    R+ + L YGP++D P YS     VH+
Sbjct:  113 FFTVKLPVALDPGSKISVVVETVYTHVLHPYPTQITQSEKQFVVFEGNHYFYSPYPTKTQTMRVKLASRNVESYTKLGNPSRSEDVLDYGPFKDIPAYSQDTFKVHY 219

HSP 2 Score: 36.1946 bits (82), Expect = 1.094e-18
Identity = 14/45 (31.11%), Postives = 27/45 (60.00%), Query Frame = 3 
Query:  405 ENNNPFAVVEELVREVDISRWGHLAITEQATCLMLVRETKACVRG 539
            ENN+PF  +  + R +++S WG++A+ E       ++ T A ++G
Sbjct:  220 ENNSPFLTITSMTRVIEVSHWGNIAVEENVD----LKHTGAVLKG 260
[Close]
BLAST of EY735627 vs. ExPASy Swiss-Prot
Match: RPN1_PONAB (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 OS=Pongo abelii GN=RPN1 PE=2 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 1.422e-18
Identity = 39/107 (36.45%), Postives = 60/107 (56.07%), Query Frame = 2 
Query:   83 YYTIVLANPLNTGETATLEILYILTHSLQPFPVEISQS*SQLVYYHDNALILSPYHIKQQTTFIKIPSTKVESFTRVEPTDRAGNELKYGPYEDRPPYSYSPLLVHF 403
            ++T+ L   L+ G   ++ +  + TH LQP+P +I+QS  Q V +  N    SPY  K QT  +K+ S  VES+T++    R+ + L YGP+ D P YS     VH+
Sbjct:  112 FFTVRLPVALDPGAKISVIVETVYTHVLQPYPTQITQSEKQFVVFEGNHYFYSPYPTKTQTMRVKLASRNVESYTKLGNPTRSEDLLDYGPFRDVPAYSQDTFKVHY 218

HSP 2 Score: 36.1946 bits (82), Expect = 1.422e-18
Identity = 14/45 (31.11%), Postives = 27/45 (60.00%), Query Frame = 3 
Query:  405 ENNNPFAVVEELVREVDISRWGHLAITEQATCLMLVRETKACVRG 539
            ENN+PF  +  + R +++S WG++A+ E       ++ T A ++G
Sbjct:  219 ENNSPFLTITSMTRVIEVSHWGNIAVEENVD----LKHTGAVLKG 259
[Close]
BLAST of EY735627 vs. ExPASy Swiss-Prot
Match: RPN1_HUMAN (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 OS=Homo sapiens GN=RPN1 PE=1 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 4.064e-18
Identity = 38/107 (35.51%), Postives = 59/107 (55.14%), Query Frame = 2 
Query:   83 YYTIVLANPLNTGETATLEILYILTHSLQPFPVEISQS*SQLVYYHDNALILSPYHIKQQTTFIKIPSTKVESFTRVEPTDRAGNELKYGPYEDRPPYSYSPLLVHF 403
            ++T+ L   L+ G   ++ +  + TH L P+P +I+QS  Q V +  N    SPY  K QT  +K+ S  VES+T++    R+ + L YGP+ D P YS     VH+
Sbjct:  112 FFTVKLPVALDPGAKISVIVETVYTHVLHPYPTQITQSEKQFVVFEGNHYFYSPYPTKTQTMRVKLASRNVESYTKLGNPTRSEDLLDYGPFRDVPAYSQDTFKVHY 218

HSP 2 Score: 36.1946 bits (82), Expect = 4.064e-18
Identity = 14/45 (31.11%), Postives = 27/45 (60.00%), Query Frame = 3 
Query:  405 ENNNPFAVVEELVREVDISRWGHLAITEQATCLMLVRETKACVRG 539
            ENN+PF  +  + R +++S WG++A+ E       ++ T A ++G
Sbjct:  219 ENNSPFLTITSMTRVIEVSHWGNIAVEENVD----LKHTGAVLKG 259
[Close]
BLAST of EY735627 vs. ExPASy Swiss-Prot
Match: RPN1_MACFA (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 OS=Macaca fascicularis GN=RPN1 PE=2 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 5.284e-18
Identity = 38/107 (35.51%), Postives = 59/107 (55.14%), Query Frame = 2 
Query:   83 YYTIVLANPLNTGETATLEILYILTHSLQPFPVEISQS*SQLVYYHDNALILSPYHIKQQTTFIKIPSTKVESFTRVEPTDRAGNELKYGPYEDRPPYSYSPLLVHF 403
            ++ + L   L+ G   ++ +  + TH LQP+P +I+QS  Q V +  N    SPY  K QT  +K+ S  VES+T++    R+ + L YGP+ D P YS     VH+
Sbjct:  112 FFIVKLPVALDPGAKISVIVETVYTHVLQPYPTQITQSEKQFVVFEGNHYFYSPYPTKTQTMRVKLASRNVESYTKLGNPTRSEDLLDYGPFRDVPAYSQDTFKVHY 218

HSP 2 Score: 36.1946 bits (82), Expect = 5.284e-18
Identity = 14/45 (31.11%), Postives = 27/45 (60.00%), Query Frame = 3 
Query:  405 ENNNPFAVVEELVREVDISRWGHLAITEQATCLMLVRETKACVRG 539
            ENN+PF  +  + R +++S WG++A+ E       ++ T A ++G
Sbjct:  219 ENNSPFLTITSMTRVIEVSHWGNIAVEENVD----LKHTGAVLKG 259
[Close]
BLAST of EY735627 vs. ExPASy Swiss-Prot
Match: RPN1_RAT (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 OS=Rattus norvegicus GN=Rpn1 PE=2 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 6.871e-18
Identity = 37/107 (34.58%), Postives = 59/107 (55.14%), Query Frame = 2 
Query:   83 YYTIVLANPLNTGETATLEILYILTHSLQPFPVEISQS*SQLVYYHDNALILSPYHIKQQTTFIKIPSTKVESFTRVEPTDRAGNELKYGPYEDRPPYSYSPLLVHF 403
            ++T+ L   L+ G   ++ +  + TH L P+P +I+QS  Q V +  N    SPY  K QT  +++ S  VES T++    R+ + L YGP++D P YS     VH+
Sbjct:  110 FFTVKLPVALDPGSKISIVVETVYTHVLHPYPTQITQSEKQFVVFEGNHYFYSPYPTKTQTMRVRLASRNVESHTKLGNPSRSEDILDYGPFKDIPAYSQDTFKVHY 216

HSP 2 Score: 36.1946 bits (82), Expect = 6.871e-18
Identity = 14/45 (31.11%), Postives = 27/45 (60.00%), Query Frame = 3 
Query:  405 ENNNPFAVVEELVREVDISRWGHLAITEQATCLMLVRETKACVRG 539
            ENN+PF  +  + R +++S WG++A+ E       ++ T A ++G
Sbjct:  217 ENNSPFLTITSMTRVIEVSHWGNIAVEENVD----LKHTGAVLKG 257
[Close]
BLAST of EY735627 vs. ExPASy Swiss-Prot
Match: OST1_DICDI (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 OS=Dictyostelium discoideum GN=ost1 PE=2 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 1.239e-16
Identity = 41/120 (34.17%), Postives = 63/120 (52.50%), Query Frame = 2 
Query:   65 APNETNY------YTIVLANPLNTGETATLEI-LYILTHSLQPFPVEISQS*SQLVYYHDNALILSPYHIKQQTTFIKIPSTKVESFTRVEPTDRAGNELKYGPYEDRPPYSYSPLLVHF 403
            +PN  N       Y I L N +   E   L++ +  +   + P+P EI Q  +QLV Y +N    SPY  + Q T +K+ S+KVES++  +PT   G  + YGPY     +S SP+ +H+
Sbjct:   90 SPNSINIKEGFKTYNIELKNKVKKDEIVQLKVKVTSMIEQMIPYPSEIKQGQTQLVLYLNNHYFTSPYKTETQKTTVKLASSKVESYSEEQPTSLKGMTVTYGPYSAIEAFSVSPMRIHY 209

HSP 2 Score: 33.8834 bits (76), Expect = 1.239e-16
Identity = 12/26 (46.15%), Postives = 20/26 (76.92%), Query Frame = 3 
Query:  405 ENNNPFAVVEELVREVDISRWGHLAI 482
            EN +PF V+  L++E ++S WG+LA+
Sbjct:  210 ENGSPFFVLTNLLKEYEVSHWGNLAV 235
The following BLAST results are available for this feature:
BLAST of EY735627 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 7
Match NameE-valueIdentityDescription
RPN1_PIG2.262e-1936.79Dolichyl-diphosphooligosaccharide--protein glycosy... [more]
RPN1_MOUSE1.094e-1835.51Dolichyl-diphosphooligosaccharide--protein glycosy... [more]
RPN1_PONAB1.422e-1836.45Dolichyl-diphosphooligosaccharide--protein glycosy... [more]
RPN1_HUMAN4.064e-1835.51Dolichyl-diphosphooligosaccharide--protein glycosy... [more]
RPN1_MACFA5.284e-1835.51Dolichyl-diphosphooligosaccharide--protein glycosy... [more]
RPN1_RAT6.871e-1834.58Dolichyl-diphosphooligosaccharide--protein glycosy... [more]
OST1_DICDI1.239e-1634.17Dolichyl-diphosphooligosaccharide--protein glycosy... [more]
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Properties
Property NameValue
Genbank descriptionCS00-C3-705-003-A01-CT.F Sweet orange fruit, development stadium (6 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY735627 ID=EY735627; Name=EY735627; organism=Citrus sinensis; type=EST; length=846bp
CCACGCGTCCGGAAGAAGAAAACGTATGTGTCACTTCGCTGTAAAACCAA
CACAACTACCTGATGCACCAAATGAAACTAATTACTACACTATTGTGTTG
GCTAATCCATTGAACACGGGTGAAACTGCAACACTAGAAATTCTTTATAT
ACTTACTCATTCTCTACAACCTTTCCCTGTGGAAATAAGCCAATCATAAT
CTCAGTTAGTATATTATCATGATAATGCTTTAATACTGTCACCGTATCAT
ATTAAGCAACAGACAACTTTCATTAAGATACCAAGTACTAAGGTGGAATC
ATTCACTAGAGTTGAACCCACCGACCGGGCAGGTAATGAACTGAAGTACG
GACCATATGAGGATCGACCTCCATATTCGTATTCTCCACTTCTTGTTCAT
TTTGGAGAATAATAACCCATTTGCTGTAGTTGAGGAGCTTGTTCGTGAAG
TGGACATATCTCGCTGGGGCCACCTTGCGATTACGGAGCAGGCAACTTGC
CTCATGCTGGTGCGCGAGACAAAGGCATGTGTGCGGGGGTTGGATCTCAC
TCCGCCCCACTTCTTATGGGGGTTGCTGCTTCCAGCACCCCAGGGCCAGC
CACCCCCTCGAGTTTCGTGCGGTGTCCTACAGAGCCAAGAGGGGAAAATC
CCTGAGTCCGGTTCCGCCTGCGACTGCGCGCACGGCGGCGGGGAGCGAGC
GGTGGGCGCGCGCCCCGTCCCCGCGACGTTGCACAACCACGCTTTCGCGA
TGCGAAGAGGGCAGGTTCTGGGCAGGAGGCGCGCGCGAGCAGAGGGGAGG
TCGGGGGGAAGGGGGTGGGAGGTGGGGGGGGGCCGTGGGGGGGGAG
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