EY735587

Overview
NameEY735587
Unique NameEY735587
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length834
Libraries
Library NameType
Sweet orange fruit, development stadium (6 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig11467 contig Csv1_Contig11467:160..993. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of EY735587 vs. ExPASy Swiss-Prot
Match: F16P2_SOLTU (Fructose-1,6-bisphosphatase, cytosolic OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 207.223 bits (526), Expect = 4.016e-62
Identity = 102/118 (86.44%), Postives = 111/118 (94.07%), Query Frame = 3 
Query:   96 MDHEADAHRTDLMTITRFVLNEQSKHPESRGDFSILLSHIVLGCKFVCSAVNKAGLAKLIGLAGETNVQGEEQKKLDVLSNDVFVKALISSGRTCILVSEEDEEAIFVEPSKRGR-CV 446
            MDH AD HRTDLMTITRFVLNEQ+KHPESRGDFSILLSHIVLGCKFVC+AVNKAGLAKL+GLAGETNVQGE+QKKLDVLSN+VF+KAL+SS RTCILVSEEDEEA FV P+ RG+ CV
Sbjct:    1 MDHAADRHRTDLMTITRFVLNEQTKHPESRGDFSILLSHIVLGCKFVCTAVNKAGLAKLLGLAGETNVQGEDQKKLDVLSNEVFIKALVSSNRTCILVSEEDEEATFVRPANRGKYCV 118

HSP 2 Score: 53.1434 bits (126), Expect = 4.016e-62
Identity = 22/24 (91.67%), Postives = 24/24 (100.00%), Query Frame = 2 
Query:  551 RYCVVFDPLDGSSNIDCGVSIGTV 622
            +YCVVFDPLDGSSNIDCGVSIGT+
Sbjct:  115 KYCVVFDPLDGSSNIDCGVSIGTI 138
[Close]
BLAST of EY735587 vs. ExPASy Swiss-Prot
Match: F16P2_SPIOL (Fructose-1,6-bisphosphatase, cytosolic OS=Spinacia oleracea PE=1 SV=2)

HSP 1 Score: 205.297 bits (521), Expect = 1.513e-61
Identity = 104/118 (88.14%), Postives = 110/118 (93.22%), Query Frame = 3 
Query:   96 MDHEADAHRTDLMTITRFVLNEQSKHPESRGDFSILLSHIVLGCKFVCSAVNKAGLAKLIGLAGETNVQGEEQKKLDVLSNDVFVKALISSGRTCILVSEEDEEAIFVEPSKRGR-CV 446
            MDH  DA RTDLMTITR+VLNEQSK PESRGDF+ILLSHIVLGCKFVCSAVNKAGLAKLIGLAGETN+QGEEQKKLDVLSN+VFVKAL SSGRTCILVSEEDEEA F+EPS RG+ CV
Sbjct:    1 MDHAGDAMRTDLMTITRYVLNEQSKRPESRGDFTILLSHIVLGCKFVCSAVNKAGLAKLIGLAGETNIQGEEQKKLDVLSNEVFVKALTSSGRTCILVSEEDEEATFIEPSLRGKYCV 118

HSP 2 Score: 53.1434 bits (126), Expect = 1.513e-61
Identity = 22/24 (91.67%), Postives = 24/24 (100.00%), Query Frame = 2 
Query:  551 RYCVVFDPLDGSSNIDCGVSIGTV 622
            +YCVVFDPLDGSSNIDCGVSIGT+
Sbjct:  115 KYCVVFDPLDGSSNIDCGVSIGTI 138
[Close]
BLAST of EY735587 vs. ExPASy Swiss-Prot
Match: F16P2_MUSAC (Fructose-1,6-bisphosphatase, cytosolic OS=Musa acuminata GN=FBPban1 PE=2 SV=1)

HSP 1 Score: 204.527 bits (519), Expect = 2.573e-61
Identity = 103/118 (87.29%), Postives = 112/118 (94.92%), Query Frame = 3 
Query:   96 MDHEADAHRTDLMTITRFVLNEQSKHPESRGDFSILLSHIVLGCKFVCSAVNKAGLAKLIGLAGETNVQGEEQKKLDVLSNDVFVKALISSGRTCILVSEEDEEAIFVEPSKRGR-CV 446
            MDHEA+AHRTDLMTITR+VLNEQS+H ESRGDF+ILLSHIVLGCKFVCSAVNKAGLAKLIGLAGETNVQG  ++KLDVLSN+VFVKALISSGRTCILVSEEDEEA FV+PS RG+ CV
Sbjct:    1 MDHEAEAHRTDLMTITRYVLNEQSRHQESRGDFTILLSHIVLGCKFVCSAVNKAGLAKLIGLAGETNVQGGSKRKLDVLSNEVFVKALISSGRTCILVSEEDEEATFVDPSLRGKYCV 118

HSP 2 Score: 53.1434 bits (126), Expect = 2.573e-61
Identity = 22/24 (91.67%), Postives = 24/24 (100.00%), Query Frame = 2 
Query:  551 RYCVVFDPLDGSSNIDCGVSIGTV 622
            +YCVVFDPLDGSSNIDCGVSIGT+
Sbjct:  115 KYCVVFDPLDGSSNIDCGVSIGTI 138
[Close]
BLAST of EY735587 vs. ExPASy Swiss-Prot
Match: F16P2_PEA (Fructose-1,6-bisphosphatase, cytosolic OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 205.297 bits (521), Expect = 7.436e-61
Identity = 104/118 (88.14%), Postives = 110/118 (93.22%), Query Frame = 3 
Query:   96 MDHEADAHRTDLMTITRFVLNEQSKHPESRGDFSILLSHIVLGCKFVCSAVNKAGLAKLIGLAGETNVQGEEQKKLDVLSNDVFVKALISSGRTCILVSEEDEEAIFVEPSKRGR-CV 446
            MDH  DA RTDLMTITR+VLNEQSK PESRGDF+ILLSHIVLGCKFVCSAVNKAGLAKLIGLAGETN+QGEEQKKLDVLSN+VFVKAL SSGRTCILVSEEDEEA F+EPS RG+ CV
Sbjct:    1 MDHAGDAMRTDLMTITRYVLNEQSKRPESRGDFTILLSHIVLGCKFVCSAVNKAGLAKLIGLAGETNIQGEEQKKLDVLSNEVFVKALTSSGRTCILVSEEDEEATFIEPSLRGKYCV 118

HSP 2 Score: 50.8322 bits (120), Expect = 7.436e-61
Identity = 21/24 (87.50%), Postives = 23/24 (95.83%), Query Frame = 2 
Query:  551 RYCVVFDPLDGSSNIDCGVSIGTV 622
            +YCVVFDPLDGS NIDCGVSIGT+
Sbjct:  115 KYCVVFDPLDGSFNIDCGVSIGTI 138
[Close]
BLAST of EY735587 vs. ExPASy Swiss-Prot
Match: F16P2_ORYCO (Fructose-1,6-bisphosphatase, cytosolic OS=Oryza coarctata PE=2 SV=1)

HSP 1 Score: 202.601 bits (514), Expect = 3.653e-60
Identity = 101/118 (85.59%), Postives = 113/118 (95.76%), Query Frame = 3 
Query:   96 MDHEADAHRTDLMTITRFVLNEQSKHPESRGDFSILLSHIVLGCKFVCSAVNKAGLAKLIGLAGETNVQGEEQKKLDVLSNDVFVKALISSGRTCILVSEEDEEAIFVEPSKRGR-CV 446
            MDHEADA+RTDLMTITR+VLNEQS++PE+RGD +ILLSHIVLGCKFV SAVNKAGLAKLIGLAGETNVQGEEQKKLDVLSN+VFVKAL+SSGRTC+LVSEEDEEA FV+P+ RG+ CV
Sbjct:    1 MDHEADAYRTDLMTITRYVLNEQSRNPEARGDLTILLSHIVLGCKFVASAVNKAGLAKLIGLAGETNVQGEEQKKLDVLSNEVFVKALVSSGRTCVLVSEEDEEATFVDPALRGKYCV 118

HSP 2 Score: 51.2174 bits (121), Expect = 3.653e-60
Identity = 21/24 (87.50%), Postives = 23/24 (95.83%), Query Frame = 2 
Query:  551 RYCVVFDPLDGSSNIDCGVSIGTV 622
            +YCV FDPLDGSSNIDCGVSIGT+
Sbjct:  115 KYCVCFDPLDGSSNIDCGVSIGTI 138
[Close]
BLAST of EY735587 vs. ExPASy Swiss-Prot
Match: F16P2_ORYSJ (Fructose-1,6-bisphosphatase, cytosolic OS=Oryza sativa subsp. japonica GN=Os01g0866400 PE=2 SV=2)

HSP 1 Score: 202.601 bits (514), Expect = 3.654e-60
Identity = 101/118 (85.59%), Postives = 113/118 (95.76%), Query Frame = 3 
Query:   96 MDHEADAHRTDLMTITRFVLNEQSKHPESRGDFSILLSHIVLGCKFVCSAVNKAGLAKLIGLAGETNVQGEEQKKLDVLSNDVFVKALISSGRTCILVSEEDEEAIFVEPSKRGR-CV 446
            MDHEADA+RTDLMTITR+VLNEQS++PE+RGD +ILLSHIVLGCKFV SAVNKAGLAKLIGLAGETNVQGEEQKKLDVLSN+VFVKAL+SSGRTC+LVSEEDEEA FV+P+ RG+ CV
Sbjct:    1 MDHEADAYRTDLMTITRYVLNEQSRNPEARGDLTILLSHIVLGCKFVASAVNKAGLAKLIGLAGETNVQGEEQKKLDVLSNEVFVKALVSSGRTCVLVSEEDEEATFVDPALRGKYCV 118

HSP 2 Score: 51.2174 bits (121), Expect = 3.654e-60
Identity = 21/24 (87.50%), Postives = 23/24 (95.83%), Query Frame = 2 
Query:  551 RYCVVFDPLDGSSNIDCGVSIGTV 622
            +YCV FDPLDGSSNIDCGVSIGT+
Sbjct:  115 KYCVCFDPLDGSSNIDCGVSIGTI 138
[Close]
BLAST of EY735587 vs. ExPASy Swiss-Prot
Match: F16P2_ORYSI (Fructose-1,6-bisphosphatase, cytosolic OS=Oryza sativa subsp. indica GN=OsI_04558 PE=2 SV=2)

HSP 1 Score: 202.601 bits (514), Expect = 3.654e-60
Identity = 101/118 (85.59%), Postives = 113/118 (95.76%), Query Frame = 3 
Query:   96 MDHEADAHRTDLMTITRFVLNEQSKHPESRGDFSILLSHIVLGCKFVCSAVNKAGLAKLIGLAGETNVQGEEQKKLDVLSNDVFVKALISSGRTCILVSEEDEEAIFVEPSKRGR-CV 446
            MDHEADA+RTDLMTITR+VLNEQS++PE+RGD +ILLSHIVLGCKFV SAVNKAGLAKLIGLAGETNVQGEEQKKLDVLSN+VFVKAL+SSGRTC+LVSEEDEEA FV+P+ RG+ CV
Sbjct:    1 MDHEADAYRTDLMTITRYVLNEQSRNPEARGDLTILLSHIVLGCKFVASAVNKAGLAKLIGLAGETNVQGEEQKKLDVLSNEVFVKALVSSGRTCVLVSEEDEEATFVDPALRGKYCV 118

HSP 2 Score: 51.2174 bits (121), Expect = 3.654e-60
Identity = 21/24 (87.50%), Postives = 23/24 (95.83%), Query Frame = 2 
Query:  551 RYCVVFDPLDGSSNIDCGVSIGTV 622
            +YCV FDPLDGSSNIDCGVSIGT+
Sbjct:  115 KYCVCFDPLDGSSNIDCGVSIGTI 138
[Close]
BLAST of EY735587 vs. ExPASy Swiss-Prot
Match: F16P2_SACHY (Fructose-1,6-bisphosphatase, cytosolic OS=Saccharum hybrid GN=CFBP PE=2 SV=2)

HSP 1 Score: 201.06 bits (510), Expect = 1.056e-59
Identity = 101/118 (85.59%), Postives = 110/118 (93.22%), Query Frame = 3 
Query:   96 MDHEADAHRTDLMTITRFVLNEQSKHPESRGDFSILLSHIVLGCKFVCSAVNKAGLAKLIGLAGETNVQGEEQKKLDVLSNDVFVKALISSGRTCILVSEEDEEAIFVEPSKRGR-CV 446
            MDH ADAHRTDLMTITR VLNEQS++PESRGDF+ILLSHIVLGCKFV SAVNKAGLA+LIGLAGETNVQGEEQKKLDVLSN+VFVKAL+SSGRTC+LVSEEDEE  FV+P  RG+ CV
Sbjct:    1 MDHAADAHRTDLMTITRHVLNEQSRNPESRGDFTILLSHIVLGCKFVASAVNKAGLAQLIGLAGETNVQGEEQKKLDVLSNEVFVKALVSSGRTCVLVSEEDEETTFVDPKLRGKYCV 118

HSP 2 Score: 51.2174 bits (121), Expect = 1.056e-59
Identity = 21/24 (87.50%), Postives = 23/24 (95.83%), Query Frame = 2 
Query:  551 RYCVVFDPLDGSSNIDCGVSIGTV 622
            +YCV FDPLDGSSNIDCGVSIGT+
Sbjct:  115 KYCVCFDPLDGSSNIDCGVSIGTI 138
[Close]
BLAST of EY735587 vs. ExPASy Swiss-Prot
Match: F16P2_ARATH (Fructose-1,6-bisphosphatase, cytosolic OS=Arabidopsis thaliana GN=At1g43670 PE=1 SV=1)

HSP 1 Score: 214.542 bits (545), Expect = 5.727e-55
Identity = 113/143 (79.02%), Postives = 123/143 (86.01%), Query Frame = 3 
Query:   96 MDHEADAHRTDLMTITRFVLNEQSKHPESRGDFSILLSHIVLGCKFVCSAVNKAGLAKLIGLAGETNVQGEEQKKLDVLSNDVFVKALISSGRTCILVSEEDEEAIFVEPSKRGR-CVPLLSVFFCSFICFKYSIYTFYTLFT 521
            MDH ADAHRTDLMTITRFVLNEQSK+PESRGDF+ILLSHIVLGCKFVCSAVNKAGLAKLIGLAGETN+QGEEQKKLDVLSNDVFV AL+SSGRT +LVSEEDEEA FVEPSKRG+ CV    +   S I    SI T + ++T
Sbjct:    1 MDHAADAHRTDLMTITRFVLNEQSKYPESRGDFTILLSHIVLGCKFVCSAVNKAGLAKLIGLAGETNIQGEEQKKLDVLSNDVFVNALVSSGRTSVLVSEEDEEATFVEPSKRGKYCVVFDPLDGSSNIDCGVSIGTIFGIYT 143
[Close]
BLAST of EY735587 vs. ExPASy Swiss-Prot
Match: F16P2_BETVU (Fructose-1,6-bisphosphatase, cytosolic OS=Beta vulgaris PE=2 SV=2)

HSP 1 Score: 213.001 bits (541), Expect = 1.666e-54
Identity = 112/142 (78.87%), Postives = 122/142 (85.92%), Query Frame = 3 
Query:   96 MDHEADAHRTDLMTITRFVLNEQSKHPESRGDFSILLSHIVLGCKFVCSAVNKAGLAKLIGLAGETNVQGEEQKKLDVLSNDVFVKALISSGRTCILVSEEDEEAIFVEPSKRGR-CVPLLSVFFCSFICFKYSIYTFYTLF 518
            MDH ADA RTDLMTITRFVLNEQSK PESRGDF+IL+SHIVLGCKFVCSAVNKAGLAKLIGLAGETN+QGEEQKKLDVLSN+VF+KALISSGRTCILVSEEDEEA FVEPS RG+ CV    +  CS I    SI T + ++
Sbjct:    1 MDHAADATRTDLMTITRFVLNEQSKRPESRGDFTILMSHIVLGCKFVCSAVNKAGLAKLIGLAGETNIQGEEQKKLDVLSNEVFIKALISSGRTCILVSEEDEEATFVEPSLRGKYCVVFDPLDGCSNIDCGVSIGTIFGIY 142
The following BLAST results are available for this feature:
BLAST of EY735587 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 198
Match NameE-valueIdentityDescription
F16P2_SOLTU4.016e-6286.44Fructose-1,6-bisphosphatase, cytosolic OS=Solanum ... [more]
F16P2_SPIOL1.513e-6188.14Fructose-1,6-bisphosphatase, cytosolic OS=Spinacia... [more]
F16P2_MUSAC2.573e-6187.29Fructose-1,6-bisphosphatase, cytosolic OS=Musa acu... [more]
F16P2_PEA7.436e-6188.14Fructose-1,6-bisphosphatase, cytosolic OS=Pisum sa... [more]
F16P2_ORYCO3.653e-6085.59Fructose-1,6-bisphosphatase, cytosolic OS=Oryza co... [more]
F16P2_ORYSJ3.654e-6085.59Fructose-1,6-bisphosphatase, cytosolic OS=Oryza sa... [more]
F16P2_ORYSI3.654e-6085.59Fructose-1,6-bisphosphatase, cytosolic OS=Oryza sa... [more]
F16P2_SACHY1.056e-5985.59Fructose-1,6-bisphosphatase, cytosolic OS=Saccharu... [more]
F16P2_ARATH5.727e-5579.02Fructose-1,6-bisphosphatase, cytosolic OS=Arabidop... [more]
F16P2_BETVU1.666e-5478.87Fructose-1,6-bisphosphatase, cytosolic OS=Beta vul... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-705-001-E03-CT.F Sweet orange fruit, development stadium (6 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY735587 ID=EY735587; Name=EY735587; organism=Citrus sinensis; type=EST; length=834bp
CCACGCGTCCGCAATCTCACGCCCTTGAATCCAGCGCTTTATCTTTGCAT
ATCTCTTAGAAAACGACAGCGTTTCTCTTTTAACCGCCGAAGAAGATGGA
TCACGAAGCGGACGCGCACCGGACGGACTTGATGACTATTACTCGTTTTG
TGCTGAACGAGCAGTCGAAGCACCCGGAGTCCCGCGGCGACTTCAGCATC
TTGCTTAGTCACATTGTTCTCGGCTGCAAGTTCGTTTGTTCTGCTGTTAA
CAAGGCGGGTCTTGCCAAGCTAATTGGACTTGCTGGAGAAACCAATGTTC
AGGGTGAAGAGCAAAAGAAATTAGATGTCCTCTCTAATGATGTCTTTGTC
AAAGCTTTGATCAGCAGTGGCCGAACATGCATCCTTGTCTCCGAAGAAGA
TGAAGAGGCAATATTTGTGGAGCCCTCTAAGCGTGGAAGGTGTGTTCCTT
TGCTCTCAGTTTTCTTTTGTTCATTTATCTGTTTTAAATATTCAATTTAT
ACTTTCTACACTCTTTTTACTTACTTGGTTCTATTTTGTTCTGATGGCAA
AGGTACTGTGTTGTTTTTGATCCGTTGGATGGGTCTTCTAACATTGATTG
TGGTGTTTCCATTGGAACGGTTTGAAACCTCATCTGAGAATAGTCTGTNT
TATGTTGTTTTATCAAAATTTTGCNAATACTGACATATGTATAAAATGAC
TATTCTTTCCAGCATGAATATTTTGCAGTGATCANTTGACTTATTCTCTT
TCATCTAGATATTTGGGATTATATGATGAAAGATACCATGACCGACTCTA
AATGATGTGTTGCACCCTGGCACCATATGTTGGG
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