EY735139 | Citrus Genome Database

Overview


Name	EY735139
Unique Name	EY735139
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	1082

Libraries


Library Name	Type
Sweet orange fruit, development stadium (5 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

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BLAST of EY735139 vs. ExPASy Swiss-Prot
Match: TPIS_ARATH (Triosephosphate isomerase, cytosolic OS=Arabidopsis thaliana GN=CTIMC PE=1 SV=2)

HSP 1 Score: 163.31 bits (412), Expect = 1.587e-43
Identity = 80/109 (73.39%), Postives = 87/109 (79.82%), Query Frame = 2

Query:  191 MARKFFVGGNWKCNGTSEEVKKIVSTLNQAXXXXXXXXXXXXXXXXXFLPLVKSVLRSDFHVAAQNCWVRKGGAFTGEVSAEMLVNLSIPWVILGHSETRDLLNDSNEF 517
            MARKFFVGGNWKCNGT+EEVKKIV+TLN+A                 FLPLVKS LRSDF VAAQNCWV+KGGAFTGEVSAEMLVNL IPWVILGHSE R +LN+S+EF
Sbjct:    1 MARKFFVGGNWKCNGTAEEVKKIVNTLNEAQVPSQDVVEVVVSPPYVFLPLVKSTLRSDFFVAAQNCWVKKGGAFTGEVSAEMLVNLDIPWVILGHSERRAILNESSEF 109

HSP 2 Score: 33.4982 bits (75), Expect = 1.587e-43
Identity = 14/19 (73.68%), Postives = 17/19 (89.47%), Query Frame = 3

Query:  516 FGGDKIAYAISQGLKGIAC 572
            F GDK+AYA++QGLK IAC
Sbjct:  109 FVGDKVAYALAQGLKVIAC 127

HSP 3 Score: 22.3274 bits (46), Expect = 1.587e-43
Identity = 10/18 (55.56%), Postives = 12/18 (66.67%), Query Frame = 1

Query:  559 KALLVVAETLEHGKAGST 612
            K +  V ETLE  +AGST
Sbjct:  123 KVIACVGETLEEREAGST 140

[Close]

BLAST of EY735139 vs. ExPASy Swiss-Prot
Match: TPIS_COPJA (Triosephosphate isomerase, cytosolic OS=Coptis japonica PE=2 SV=1)

HSP 1 Score: 163.31 bits (412), Expect = 2.063e-43
Identity = 79/109 (72.48%), Postives = 86/109 (78.90%), Query Frame = 2

Query:  191 MARKFFVGGNWKCNGTSEEVKKIVSTLNQAXXXXXXXXXXXXXXXXXFLPLVKSVLRSDFHVAAQNCWVRKGGAFTGEVSAEMLVNLSIPWVILGHSETRDLLNDSNEF 517
            M RKFFVGGNWKCNGTSEEVKKIV+ LN+A                 FLP VK++LR+DFHVAAQNCWV+KGGAFTGEVSAEMLVNL IPWVILGHSE R LLN+SNEF
Sbjct:    1 MGRKFFVGGNWKCNGTSEEVKKIVTLLNEAEVPSEDVVEVVVSPPYVFLPFVKNLLRADFHVAAQNCWVKKGGAFTGEVSAEMLVNLGIPWVILGHSERRALLNESNEF 109

HSP 2 Score: 33.113 bits (74), Expect = 2.063e-43
Identity = 15/19 (78.95%), Postives = 16/19 (84.21%), Query Frame = 3

Query:  516 FGGDKIAYAISQGLKGIAC 572
            F GDK AYA+SQGLK IAC
Sbjct:  109 FVGDKTAYALSQGLKVIAC 127

HSP 3 Score: 22.3274 bits (46), Expect = 2.063e-43
Identity = 10/18 (55.56%), Postives = 12/18 (66.67%), Query Frame = 1

Query:  559 KALLVVAETLEHGKAGST 612
            K +  V ETLE  +AGST
Sbjct:  123 KVIACVGETLEQREAGST 140

[Close]

BLAST of EY735139 vs. ExPASy Swiss-Prot
Match: TPIS_PETHY (Triosephosphate isomerase, cytosolic OS=Petunia hybrida GN=TPIP1 PE=2 SV=1)

HSP 1 Score: 160.999 bits (406), Expect = 2.284e-43
Identity = 79/109 (72.48%), Postives = 85/109 (77.98%), Query Frame = 2

Query:  191 MARKFFVGGNWKCNGTSEEVKKIVSTLNQAXXXXXXXXXXXXXXXXXFLPLVKSVLRSDFHVAAQNCWVRKGGAFTGEVSAEMLVNLSIPWVILGHSETRDLLNDSNEF 517
            M RKFFVGGNWKCNGT+EEVKKI++TLN A                 FLPLVK+ LR DFHVAAQNCWV+KGGAFTGEVSAEMLVNLSIPWVILGHSE R LL +SNEF
Sbjct:    1 MGRKFFVGGNWKCNGTAEEVKKILATLNAADVPSQDVVEVVVSPPYVFLPLVKNELRPDFHVAAQNCWVKKGGAFTGEVSAEMLVNLSIPWVILGHSERRALLGESNEF 109

HSP 2 Score: 36.965 bits (84), Expect = 2.284e-43
Identity = 31/89 (34.83%), Postives = 45/89 (50.56%), Query Frame = 3

Query:  516 FGGDKIAYAISQGLKGIACGCRNSGTRKSRIYRGLVVADQNDTNPRKRNAKLGTMLVDDLRGAE*WASGYTGKASNTPARRDSQNTHAQ 782
            F GDK+AYA+SQGLK IAC       R+S     +V A       R ++     +  + +     WA G TGK + +PA+  +Q  HA+
Sbjct:  109 FVGDKVAYALSQGLKVIACVGETLEERESGSTMDVVAAQTKAIADRVKDWTNVVVAYEPV-----WAIG-TGKVA-SPAQ--AQEVHAE 188

[Close]

BLAST of EY735139 vs. ExPASy Swiss-Prot
Match: TPIS_MAIZE (Triosephosphate isomerase, cytosolic OS=Zea mays PE=3 SV=3)

HSP 1 Score: 154.451 bits (389), Expect = 5.929e-41
Identity = 73/109 (66.97%), Postives = 83/109 (76.15%), Query Frame = 2

Query:  191 MARKFFVGGNWKCNGTSEEVKKIVSTLNQAXXXXXXXXXXXXXXXXXFLPLVKSVLRSDFHVAAQNCWVRKGGAFTGEVSAEMLVNLSIPWVILGHSETRDLLNDSNEF 517
            M RKFFVGGNWKCNGT+++V+KIV TLN+                  FLP+VKS LR +FHVAAQNCWV+KGGAFTGEVSAEMLVNL +PWVILGHSE R LL +SNEF
Sbjct:    1 MGRKFFVGGNWKCNGTTDQVEKIVKTLNEGQVPPSDVVEVVVSPPYVFLPVVKSQLRQEFHVAAQNCWVKKGGAFTGEVSAEMLVNLGVPWVILGHSERRALLGESNEF 109

HSP 2 Score: 35.4242 bits (80), Expect = 5.929e-41
Identity = 30/88 (34.09%), Postives = 44/88 (50.00%), Query Frame = 3

Query:  516 FGGDKIAYAISQGLKGIACGCRNSGTRKSRIYRGLVVADQNDTNPRKRNAKLGTMLVDDLRGAE*WASGYTGKASNTPARRDSQNTHA 779
            F GDK+AYA+SQGLK IAC       R++     +V A       + ++     +  + +     WA G TGK + TPA+  +Q  HA
Sbjct:  109 FVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIAEKIKDWSNVVVAYEPV-----WAIG-TGKVA-TPAQ--AQEVHA 187

[Close]

BLAST of EY735139 vs. ExPASy Swiss-Prot
Match: TPIS_ORYSJ (Triosephosphate isomerase, cytosolic OS=Oryza sativa subsp. japonica GN=TPI PE=1 SV=3)

HSP 1 Score: 148.288 bits (373), Expect = 5.328e-39
Identity = 72/109 (66.06%), Postives = 80/109 (73.39%), Query Frame = 2

Query:  191 MARKFFVGGNWKCNGTSEEVKKIVSTLNQAXXXXXXXXXXXXXXXXXFLPLVKSVLRSDFHVAAQNCWVRKGGAFTGEVSAEMLVNLSIPWVILGHSETRDLLNDSNEF 517
            M RKFFVGGNWKCNGT+++V KIV  LN+                  FLP+VKS LR +  VAAQNCWV+KGGAFTGEVSAEMLVNLSIPWVILGHSE R LL +SNEF
Sbjct:    1 MGRKFFVGGNWKCNGTTDQVDKIVKILNEGQIASTDVVEVVVSPPYVFLPVVKSQLRPEIQVAAQNCWVKKGGAFTGEVSAEMLVNLSIPWVILGHSERRSLLGESNEF 109

HSP 2 Score: 35.039 bits (79), Expect = 5.328e-39
Identity = 20/46 (43.48%), Postives = 25/46 (54.35%), Query Frame = 3

Query:  516 FGGDKIAYAISQGLKGIACGCRNSGTRKSRIYRGLVVADQNDTNPR 653
            F GDK+AYA+SQGLK IAC       R+S     +V A     + R
Sbjct:  109 FVGDKVAYALSQGLKVIACVGETLEQRESGSTMDVVAAQTKAISER 154

[Close]

BLAST of EY735139 vs. ExPASy Swiss-Prot
Match: TPIS_HORVU (Triosephosphate isomerase, cytosolic OS=Hordeum vulgare PE=1 SV=3)

HSP 1 Score: 141.739 bits (356), Expect = 1.377e-36
Identity = 68/109 (62.39%), Postives = 77/109 (70.64%), Query Frame = 2

Query:  191 MARKFFVGGNWKCNGTSEEVKKIVSTLNQAXXXXXXXXXXXXXXXXXFLPLVKSVLRSDFHVAAQNCWVRKGGAFTGEVSAEMLVNLSIPWVILGHSETRDLLNDSNEF 517
            M RKFFVGGNWKCNGT E+V+ IV TLN                   FLP+VK+ LR +  VAAQNCWV+KGGAFTGEVSAEML NL +PWVILGHSE R LL +S+EF
Sbjct:    1 MGRKFFVGGNWKCNGTVEQVEAIVQTLNAGQIVSPDVVEVVVSPPYVFLPIVKAKLRPEIQVAAQNCWVKKGGAFTGEVSAEMLANLGVPWVILGHSERRSLLGESSEF 109

HSP 2 Score: 33.4982 bits (75), Expect = 1.377e-36
Identity = 28/88 (31.82%), Postives = 44/88 (50.00%), Query Frame = 3

Query:  516 FGGDKIAYAISQGLKGIACGCRNSGTRKSRIYRGLVVADQNDTNPRKRNAKLGTMLVDDLRGAE*WASGYTGKASNTPARRDSQNTHA 779
            F G+K+AYA++QGLK IAC       R++     +V         + ++   G +  + +     WA G TGK + TPA+  +Q  HA
Sbjct:  109 FVGEKVAYALAQGLKVIACVGETLEQREAGSTMEVVAEQTKAIAGKIKDWSNGVVAYEPV-----WAIG-TGKVA-TPAQ--AQEVHA 187

[Close]

BLAST of EY735139 vs. ExPASy Swiss-Prot
Match: TPIS_SECCE (Triosephosphate isomerase, cytosolic OS=Secale cereale PE=2 SV=3)

HSP 1 Score: 139.813 bits (351), Expect = 4.056e-36
Identity = 69/109 (63.30%), Postives = 76/109 (69.72%), Query Frame = 2

Query:  191 MARKFFVGGNWKCNGTSEEVKKIVSTLNQAXXXXXXXXXXXXXXXXXFLPLVKSVLRSDFHVAAQNCWVRKGGAFTGEVSAEMLVNLSIPWVILGHSETRDLLNDSNEF 517
            M RKFFVGGNWKCNGT  +V+ IV+TLN                   FLP VK  LR +  VAAQNCWV+KGGAFTGEVSAEMLVNL IPWVILGHSE R LL +S+EF
Sbjct:    1 MGRKFFVGGNWKCNGTVSQVETIVNTLNAGQIASPDVVEVVVSPPYVFLPTVKDKLRPEIQVAAQNCWVKKGGAFTGEVSAEMLVNLGIPWVILGHSERRSLLAESSEF 109

HSP 2 Score: 31.9574 bits (71), Expect = 4.056e-36
Identity = 13/19 (68.42%), Postives = 17/19 (89.47%), Query Frame = 3

Query:  516 FGGDKIAYAISQGLKGIAC 572
            F G+K+AYA++QGLK IAC
Sbjct:  109 FVGEKVAYALAQGLKVIAC 127

HSP 3 Score: 22.3274 bits (46), Expect = 4.056e-36
Identity = 10/18 (55.56%), Postives = 12/18 (66.67%), Query Frame = 1

Query:  559 KALLVVAETLEHGKAGST 612
            K +  V ETLE  +AGST
Sbjct:  123 KVIACVGETLEQREAGST 140

[Close]

BLAST of EY735139 vs. ExPASy Swiss-Prot
Match: TPIS_STELP (Triosephosphate isomerase, cytosolic OS=Stellaria longipes GN=TPI PE=2 SV=1)

HSP 1 Score: 134.42 bits (337), Expect = 9.103e-32
Identity = 66/109 (60.55%), Postives = 73/109 (66.97%), Query Frame = 2

Query:  191 MARKFFVGGNWKCNGTSEEVKKIVSTLNQAXXXXXXXXXXXXXXXXXFLPLVKSVLRSDFHVAAQNCWVRKGGAFTGEVSAEMLVNLSIPWVILGHSETRDLLNDSNEF 517
            M RKFFVGGNWKCNGT E V KIV TLN+                  FLP   + L+ +  VAAQNCWV+KGGAFTGEVSA+ML NL I WVILGHSE R LL +SNEF
Sbjct:    1 MGRKFFVGGNWKCNGTQESVSKIVDTLNEPTIAANDVVTVVVSPPYVFLPDENAELKHEIQVAAQNCWVKKGGAFTGEVSAQMLANLGITWVILGHSERRTLLGESNEF 109

HSP 2 Score: 24.6386 bits (52), Expect = 9.103e-32
Identity = 12/19 (63.16%), Postives = 13/19 (68.42%), Query Frame = 3

Query:  516 FGGDKIAYAISQGLKGIAC 572
            F G K AYA S+GL  IAC
Sbjct:  109 FVGKKAAYAQSEGLGVIAC 127

[Close]

BLAST of EY735139 vs. ExPASy Swiss-Prot
Match: TPIC_SPIOL (Triosephosphate isomerase, chloroplastic OS=Spinacia oleracea GN=TPIP1 PE=1 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 8.835e-25
Identity = 53/106 (50.00%), Postives = 65/106 (61.32%), Query Frame = 2

Query:  200 KFFVGGNWKCNGTSEEVKKIVSTLNQAXXXXXXXXXXXXXXXXXFLPLVKSVLRSDFHVAAQNCWVRKGGAFTGEVSAEMLVNLSIPWVILGHSETRDLLNDSNEF 517
            KFFVGGNWKCNGT E + K+VS LN A                 ++  VKS L     ++AQNCW+ KGGAFTGE+S E L +L   WVILGHSE R ++ + NEF
Sbjct:   72 KFFVGGNWKCNGTKESITKLVSDLNSATLEADVDVVVAPPFV--YIDQVKSSLTGRVEISAQNCWIGKGGAFTGEISVEQLKDLGCQWVILGHSERRHVIGEQNEF 175

HSP 2 Score: 26.1794 bits (56), Expect = 8.835e-25
Identity = 12/22 (54.55%), Postives = 15/22 (68.18%), Query Frame = 3

Query:  507 QMNFGGDKIAYAISQGLKGIAC 572
            Q  F G K AYA++QG+  IAC
Sbjct:  172 QNEFIGKKAAYALNQGVGVIAC 193

[Close]

BLAST of EY735139 vs. ExPASy Swiss-Prot
Match: TPIC_SECCE (Triosephosphate isomerase, chloroplastic OS=Secale cereale PE=1 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 1.499e-24
Identity = 57/114 (50.00%), Postives = 70/114 (61.40%), Query Frame = 2

Query:  182 LVVMAR--KFFVGGNWKCNGTSEEVKKIVSTLNQAXXXXXXXXXXXXXXXXXFLPLVKSVLRSDFHVAAQNCWVRKGGAFTGEVSAEMLVNLSIPWVILGHSETRDLLNDSNEF 517
            LV MA   KFFVGGNWKCNGT E + K+VS LN A                 ++  VKS L     V+AQN W+ KGGAFTGE+SAE LV++   WVILGHSE R ++ + +EF
Sbjct:   40 LVAMAGSGKFFVGGNWKCNGTKESISKLVSDLNAATLESDVDVVVAPPFI--YIDQVKSSLTDRIEVSAQNTWIGKGGAFTGEISAEQLVDIGCQWVILGHSERRHVIGEDDEF 151

HSP 2 Score: 26.5646 bits (57), Expect = 1.499e-24
Identity = 12/19 (63.16%), Postives = 14/19 (73.68%), Query Frame = 3

Query:  516 FGGDKIAYAISQGLKGIAC 572
            F G K AYA+SQ LK +AC
Sbjct:  151 FIGKKAAYALSQNLKVMAC 169

The following BLAST results are available for this feature:

BLAST of EY735139 vs. ExPASy Swiss-Prot

BLAST of EY735139 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 89


Match Name	E-value	Identity	Description
TPIS_ARATH	1.587e-43	73.39	Triosephosphate isomerase, cytosolic OS=Arabidopsi...	[more]
TPIS_COPJA	2.063e-43	72.48	Triosephosphate isomerase, cytosolic OS=Coptis jap...	[more]
TPIS_PETHY	2.284e-43	72.48	Triosephosphate isomerase, cytosolic OS=Petunia hy...	[more]
TPIS_MAIZE	5.929e-41	66.97	Triosephosphate isomerase, cytosolic OS=Zea mays P...	[more]
TPIS_ORYSJ	5.328e-39	66.06	Triosephosphate isomerase, cytosolic OS=Oryza sati...	[more]
TPIS_HORVU	1.377e-36	62.39	Triosephosphate isomerase, cytosolic OS=Hordeum vu...	[more]
TPIS_SECCE	4.056e-36	63.30	Triosephosphate isomerase, cytosolic OS=Secale cer...	[more]
TPIS_STELP	9.103e-32	60.55	Triosephosphate isomerase, cytosolic OS=Stellaria ...	[more]
TPIC_SPIOL	8.835e-25	50.00	Triosephosphate isomerase, chloroplastic OS=Spinac...	[more]
TPIC_SECCE	1.499e-24	50.00	Triosephosphate isomerase, chloroplastic OS=Secale...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C3-704-082-H06-CT.F Sweet orange fruit, development stadium (5 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY735139 ID=EY735139; Name=EY735139; organism=Citrus sinensis; type=EST; length=1082bp
GGGGNAAGGAANGAGAGGGAAGGAGGGAGGTGTACACTAATACGAAAGAT
NCNCATGGTAGCCTGCAGGTACGGNCCGGAAATCCCGTTACAAACGAGCG
TCCGCGGACGCGTGTTTTGNTCCAAGAAGTTGATTTCTCGTATTTTCGTC
TCGCTTTCATCGCGAAACCAAACTATTTCCACTCGTCGTCATGGCCCGCA
AATTCTTCGTCGGCGGCAACTGGAAATGCAATGGGACATCTGAGGAAGTG
AAGAAGATTGTTTCTACATTGAATCAAGCTGAAGTTCCTTCTGAGGATGT
TGTGGAGGTTGTTGTAAGCCCTCCGATTGTGTTTCTTCCTTTGGTCAAGA
GTGTGTTGCGGTCTGATTTCCATGTTGCTGCTCAAAATTGCTGGGTTCGC
AAAGGTGGTGCTTTTACCGGGGAAGTTAGTGCTGAGATGCTTGTCAACTT
GAGCATTCCTTGGGTCATTCTTGGTCATTCTGAAACAAGAGATCTTTTGA
ATGACTCAAATGAATTTTGGTGGAGATAAAATTGCTTATGCAATTTCTCA
AGGCTTGAAAGGCATTGCTTGTGGTTGCAGAAACTCTGGAACACGGAAAA
GCAGGATCTACCGGGGCCTGGTTGTGGCTGACCAGAACGACACGAATCCG
CGGAAAAGGAATGCCAAATTGGGGACAATGTTAGTGGATGACCTAAGGGG
AGCCGAATAGTGGGCCAGTGGCTACACAGGGAAGGCTAGCAACACCCCCG
CACGGCGCGACTCGCAAAACACGCACGCACAACGCCCCACAAGCACGACT
GCACAAACAACCCCCAGGGAGCCGCGCGCGGCGCGGCCTAGCCGGGCGCC
CCGAGCGTGAAGGGCCGACCGCGGCGNATAGAGTGGGGAGAAAAAGGTAC
AGCGGAGCAGTAAAAAACCGAGAATAAAGAAGAAGAAAGCATAAAGGATG
AAAGAAGAAAGACTACGATGAGACCATGCAGCGGAGAGACGAATAACGAG
GCACCCCATACCCGGGACACAGTGATGCGCCAGCACGACGCCAGACCGCG
GCGAGAGACTAAACACACCAAGCACATATCCA