EY731477

Overview
NameEY731477
Unique NameEY731477
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length834
Libraries
Library NameType
Sweet orange fruit, development stadium (5 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY731477 vs. ExPASy Swiss-Prot
Match: GAO1A_WHEAT (Gibberellin 20 oxidase 1-A OS=Triticum aestivum GN=GA20ox1A PE=2 SV=1)

HSP 1 Score: 132.109 bits (331), Expect = 3.794e-60
Identity = 59/86 (68.60%), Postives = 70/86 (81.40%), Query Frame = 2 
Query:  227 FMALSNGIYKNCLHRTAVNNTTVRKSLAFFLCPKMDKVVTPPNNLVNANNPRIYPDFTWHNLLHFTQNYFRADVKTLNAFSAWLQQ 484
            FMALSNG YK+CLHR  VN+   RKSLAFFLCP+MDKVV PP  LV+A+NPR YPDFTW +LL FTQ ++RAD+KTL  FS+W+ Q
Sbjct:  267 FMALSNGRYKSCLHRAVVNSRVPRKSLAFFLCPEMDKVVAPPGTLVDASNPRAYPDFTWRSLLDFTQKHYRADMKTLEVFSSWIVQ 352

HSP 2 Score: 119.398 bits (298), Expect = 3.794e-60
Identity = 52/74 (70.27%), Postives = 62/74 (83.78%), Query Frame = 1 
Query:    1 HASGIFQGHDSIMRLNYYPPCQKPNLTLGTGPHCDPTSLTILHQDQVGGLQVYVDEQWQSVSPNPNAFVVNIGE 222
            H    F+G+DSIMRLNYYPPCQ+P  TLGTGPHCDPTSLTILHQD VGGLQV+ + +W+S+ P  +AFVVNIG+
Sbjct:  192 HYRRFFEGNDSIMRLNYYPPCQRPLETLGTGPHCDPTSLTILHQDNVGGLQVHTEGRWRSIRPRADAFVVNIGD 265
[Close]
BLAST of EY731477 vs. ExPASy Swiss-Prot
Match: GAO1B_WHEAT (Gibberellin 20 oxidase 1-B OS=Triticum aestivum GN=GA20ox1B PE=2 SV=1)

HSP 1 Score: 130.954 bits (328), Expect = 6.450e-60
Identity = 59/86 (68.60%), Postives = 69/86 (80.23%), Query Frame = 2 
Query:  227 FMALSNGIYKNCLHRTAVNNTTVRKSLAFFLCPKMDKVVTPPNNLVNANNPRIYPDFTWHNLLHFTQNYFRADVKTLNAFSAWLQQ 484
            FMALSNG YK+CLHR  VN+   RKSLAFFLCP+MDKVV PP  LV+A NPR YPDFTW +LL FTQ ++RAD+KTL  FS+W+ Q
Sbjct:  267 FMALSNGRYKSCLHRAVVNSKVPRKSLAFFLCPEMDKVVAPPGTLVDAANPRAYPDFTWRSLLDFTQKHYRADMKTLEVFSSWIVQ 352

HSP 2 Score: 119.783 bits (299), Expect = 6.450e-60
Identity = 52/74 (70.27%), Postives = 62/74 (83.78%), Query Frame = 1 
Query:    1 HASGIFQGHDSIMRLNYYPPCQKPNLTLGTGPHCDPTSLTILHQDQVGGLQVYVDEQWQSVSPNPNAFVVNIGE 222
            H    F+G+DSIMRLNYYPPCQ+P  TLGTGPHCDPTSLTILHQD VGGLQV+ + +W+S+ P  +AFVVNIG+
Sbjct:  192 HYRRFFEGNDSIMRLNYYPPCQRPMETLGTGPHCDPTSLTILHQDNVGGLQVHTEGRWRSIRPRADAFVVNIGD 265
[Close]
BLAST of EY731477 vs. ExPASy Swiss-Prot
Match: GAO1D_WHEAT (Gibberellin 20 oxidase 1-D OS=Triticum aestivum GN=GA20ox1D PE=1 SV=1)

HSP 1 Score: 130.954 bits (328), Expect = 8.411e-60
Identity = 59/86 (68.60%), Postives = 69/86 (80.23%), Query Frame = 2 
Query:  227 FMALSNGIYKNCLHRTAVNNTTVRKSLAFFLCPKMDKVVTPPNNLVNANNPRIYPDFTWHNLLHFTQNYFRADVKTLNAFSAWLQQ 484
            FMALSNG YK+CLHR  VN+   RKSLAFFLCP+MDKVV PP  LV+A NPR YPDFTW +LL FTQ ++RAD+KTL  FS+W+ Q
Sbjct:  267 FMALSNGRYKSCLHRAVVNSRVPRKSLAFFLCPEMDKVVAPPGTLVDAANPRAYPDFTWRSLLDFTQKHYRADMKTLEVFSSWIVQ 352

HSP 2 Score: 119.398 bits (298), Expect = 8.411e-60
Identity = 52/74 (70.27%), Postives = 62/74 (83.78%), Query Frame = 1 
Query:    1 HASGIFQGHDSIMRLNYYPPCQKPNLTLGTGPHCDPTSLTILHQDQVGGLQVYVDEQWQSVSPNPNAFVVNIGE 222
            H    F+G+DSIMRLNYYPPCQ+P  TLGTGPHCDPTSLTILHQD VGGLQV+ + +W+S+ P  +AFVVNIG+
Sbjct:  192 HYRRFFEGNDSIMRLNYYPPCQRPLETLGTGPHCDPTSLTILHQDNVGGLQVHTEGRWRSIRPRADAFVVNIGD 265
[Close]
BLAST of EY731477 vs. ExPASy Swiss-Prot
Match: GAOX2_ARATH (Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana GN=20ox2 PE=2 SV=1)

HSP 1 Score: 127.102 bits (318), Expect = 1.299e-57
Identity = 55/71 (77.46%), Postives = 63/71 (88.73%), Query Frame = 1 
Query:   10 GIFQGHDSIMRLNYYPPCQKPNLTLGTGPHCDPTSLTILHQDQVGGLQVYVDEQWQSVSPNPNAFVVNIGE 222
            G F+ +DSIMRLN+YPPCQ P+LTLGTGPHCDP+SLTILHQD V GLQV+VD QWQS+ PNP AFVVNIG+
Sbjct:  216 GFFEENDSIMRLNHYPPCQTPDLTLGTGPHCDPSSLTILHQDHVNGLQVFVDNQWQSIRPNPKAFVVNIGD 286

HSP 2 Score: 115.931 bits (289), Expect = 1.299e-57
Identity = 51/84 (60.71%), Postives = 64/84 (76.19%), Query Frame = 2 
Query:  227 FMALSNGIYKNCLHRTAVNNTTVRKSLAFFLCPKMDKVVTPPNNLVNANNPRIYPDFTWHNLLHFTQNYFRADVKTLNAFSAWL 478
            FMALSNGI+K+CLHR  VN  + RKS+AFFLCPK DKVV PP++++     R YPDFTW   L FTQ ++RADV TL++FS W+
Sbjct:  288 FMALSNGIFKSCLHRAVVNRESARKSMAFFLCPKKDKVVKPPSDILEKMKTRKYPDFTWSMFLEFTQKHYRADVNTLDSFSNWV 371
[Close]
BLAST of EY731477 vs. ExPASy Swiss-Prot
Match: GAOX1_ARATH (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana GN=20ox1 PE=2 SV=2)

HSP 1 Score: 124.405 bits (311), Expect = 1.084e-56
Identity = 54/69 (78.26%), Postives = 62/69 (89.86%), Query Frame = 1 
Query:   16 FQGHDSIMRLNYYPPCQKPNLTLGTGPHCDPTSLTILHQDQVGGLQVYVDEQWQSVSPNPNAFVVNIGE 222
            F+ +DSIMRLNYYPPC KP+LTLGTGPHCDPTSLTILHQD V GLQV+V+ QW+S+ PNP AFVVNIG+
Sbjct:  220 FEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRSIRPNPKAFVVNIGD 288

HSP 2 Score: 115.546 bits (288), Expect = 1.084e-56
Identity = 53/88 (60.23%), Postives = 65/88 (73.86%), Query Frame = 2 
Query:  227 FMALSNGIYKNCLHRTAVNNTTVRKSLAFFLCPKMDKVVTPPNNLVNANNPRIYPDFTWHNLLHFTQNYFRADVKTLNAFSAWLQQHV 490
            FMALSN  YK+CLHR  VN+ + RKSLAFFLCPK D+VVTPP  L+++   R YPDFTW   L FTQ ++RAD+ TL AFS WL + +
Sbjct:  290 FMALSNDRYKSCLHRAVVNSESERKSLAFFLCPKKDRVVTPPRELLDSITSRRYPDFTWSMFLEFTQKHYRADMNTLQAFSDWLTKPI 377
[Close]
BLAST of EY731477 vs. ExPASy Swiss-Prot
Match: GAOX1_ORYSJ (Gibberellin 20 oxidase 1 OS=Oryza sativa subsp. japonica GN=20ox1 PE=2 SV=2)

HSP 1 Score: 121.709 bits (304), Expect = 2.180e-54
Identity = 53/90 (58.89%), Postives = 68/90 (75.56%), Query Frame = 2 
Query:  227 FMALSNGIYKNCLHRTAVNNTTVRKSLAFFLCPKMDKVVTPPNNLVNANNPRIYPDFTWHNLLHFTQNYFRADVKTLNAFSAWLQQHVYI 496
            FMALSN  Y++CLHR  VN+T  R+SLAFFLCP+MD VV PP  LV+ ++PR+YPDFTW  LL FTQ ++RAD++   AFS WL  H ++
Sbjct:  277 FMALSNARYRSCLHRAVVNSTAPRRSLAFFLCPEMDTVVRPPEELVDDHHPRVYPDFTWRALLDFTQRHYRADMRLFQAFSDWLNHHRHL 366

HSP 2 Score: 110.538 bits (275), Expect = 2.180e-54
Identity = 47/69 (68.12%), Postives = 58/69 (84.06%), Query Frame = 1 
Query:   16 FQGHDSIMRLNYYPPCQKPNLTLGTGPHCDPTSLTILHQDQVGGLQVYVDEQWQSVSPNPNAFVVNIGE 222
            FQ +DSIMRLNYYP CQ+P  TLGTGPHCDPTSLTILHQD VGGL+V+ + +W+++ P P A VVN+G+
Sbjct:  207 FQRNDSIMRLNYYPACQRPLDTLGTGPHCDPTSLTILHQDHVGGLEVWAEGRWRAIRPRPGALVVNVGD 275
[Close]
BLAST of EY731477 vs. ExPASy Swiss-Prot
Match: GAOX3_ARATH (Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana GN=20ox3 PE=2 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 4.829e-54
Identity = 53/69 (76.81%), Postives = 61/69 (88.41%), Query Frame = 1 
Query:   16 FQGHDSIMRLNYYPPCQKPNLTLGTGPHCDPTSLTILHQDQVGGLQVYVDEQWQSVSPNPNAFVVNIGE 222
            F+  DSI RLNYYP C++P L LGTGPHCDPTSLTILHQDQVGGLQV+VD +WQS+ PNP+AFVVNIG+
Sbjct:  219 FEDSDSIFRLNYYPQCKQPELALGTGPHCDPTSLTILHQDQVGGLQVFVDNKWQSIPPNPHAFVVNIGD 287

HSP 2 Score: 108.227 bits (269), Expect = 4.829e-54
Identity = 51/87 (58.62%), Postives = 62/87 (71.26%), Query Frame = 2 
Query:  227 FMALSNGIYKNCLHRTAVNNTTVRKSLAFFLCPKMDKVVTPPNNLVNA--NNPRIYPDFTWHNLLHFTQNYFRADVKTLNAFSAWLQ 481
            FMAL+NG YK+CLHR  VN+   RK+ AFFLCPK +KVV PP  LVN   +  R YPDFTW   L FTQ ++RAD+ TL+ FS WL+
Sbjct:  289 FMALTNGRYKSCLHRAVVNSERERKTFAFFLCPKGEKVVKPPEELVNGVKSGERKYPDFTWSMFLEFTQKHYRADMNTLDEFSIWLK 375
[Close]
BLAST of EY731477 vs. ExPASy Swiss-Prot
Match: GAOX2_ORYSJ (Gibberellin 20 oxidase 2 OS=Oryza sativa subsp. japonica GN=20ox2 PE=1 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 3.584e-48
Identity = 50/84 (59.52%), Postives = 63/84 (75.00%), Query Frame = 2 
Query:  227 FMALSNGIYKNCLHRTAVNNTTVRKSLAFFLCPKMDKVVTPPNNLVNANNPRIYPDFTWHNLLHFTQNYFRADVKTLNAFSAWL 478
            FMALSNG YK+CLHR  VN    R+SLAFFLCP+ D+VV PP    +A  P+ YPDFTW +L+ FTQ ++RAD +TL+AF+ WL
Sbjct:  292 FMALSNGRYKSCLHRAVVNQRRERRSLAFFLCPREDRVVRPP---PSAATPQHYPDFTWADLMRFTQRHYRADTRTLDAFTRWL 372

HSP 2 Score: 103.219 bits (256), Expect = 3.584e-48
Identity = 46/69 (66.67%), Postives = 53/69 (76.81%), Query Frame = 1 
Query:   16 FQGHDSIMRLNYYPPCQKPNLTLGTGPHCDPTSLTILHQDQVGGLQVYVDEQWQSVSPNPNAFVVNIGE 222
            F    SIMR NYYPPC +P  TLGTGPHCDPT+LTIL QD VGGL+V VD +W+ VSP P A V+NIG+
Sbjct:  222 FADSSSIMRCNYYPPCPEPERTLGTGPHCDPTALTILLQDDVGGLEVLVDGEWRPVSPVPGAMVINIGD 290
[Close]
BLAST of EY731477 vs. ExPASy Swiss-Prot
Match: GAOX2_ORYSI (Gibberellin 20 oxidase 2 OS=Oryza sativa subsp. indica GN=20ox2 PE=1 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 3.584e-48
Identity = 50/84 (59.52%), Postives = 63/84 (75.00%), Query Frame = 2 
Query:  227 FMALSNGIYKNCLHRTAVNNTTVRKSLAFFLCPKMDKVVTPPNNLVNANNPRIYPDFTWHNLLHFTQNYFRADVKTLNAFSAWL 478
            FMALSNG YK+CLHR  VN    R+SLAFFLCP+ D+VV PP    +A  P+ YPDFTW +L+ FTQ ++RAD +TL+AF+ WL
Sbjct:  292 FMALSNGRYKSCLHRAVVNQRRERRSLAFFLCPREDRVVRPP---PSAATPQHYPDFTWADLMRFTQRHYRADTRTLDAFTRWL 372

HSP 2 Score: 103.219 bits (256), Expect = 3.584e-48
Identity = 46/69 (66.67%), Postives = 53/69 (76.81%), Query Frame = 1 
Query:   16 FQGHDSIMRLNYYPPCQKPNLTLGTGPHCDPTSLTILHQDQVGGLQVYVDEQWQSVSPNPNAFVVNIGE 222
            F    SIMR NYYPPC +P  TLGTGPHCDPT+LTIL QD VGGL+V VD +W+ VSP P A V+NIG+
Sbjct:  222 FADSSSIMRCNYYPPCPEPERTLGTGPHCDPTALTILLQDDVGGLEVLVDGEWRPVSPVPGAMVINIGD 290
[Close]
BLAST of EY731477 vs. ExPASy Swiss-Prot
Match: G2OX8_ARATH (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana GN=GA2OX7 PE=1 SV=2)

HSP 1 Score: 79.7221 bits (195), Expect = 4.297e-21
Identity = 34/62 (54.84%), Postives = 44/62 (70.97%), Query Frame = 1 
Query:   37 MRLNYYPPCQKPNLTLGTGPHCDPTSLTILHQDQVGGLQVYVDEQWQSVSPNPNAFVVNIGE 222
            +R+N YPPC KP+   G  PH D   LTIL+QDQVGGLQ+  D +W +V PNP A ++NIG+
Sbjct:  195 LRMNRYPPCPKPSEVYGLMPHTDSDFLTILYQDQVGGLQLIKDNRWIAVKPNPKALIINIGD 256

HSP 2 Score: 40.817 bits (94), Expect = 4.297e-21
Identity = 18/39 (46.15%), Postives = 23/39 (58.97%), Query Frame = 2 
Query:  227 FMALSNGIYKNCLHRTAVNNTTVRKSLAFFLCPKMDKVV 343
            F A SNG+YK+  HR   N    R S A+F+CP  D V+
Sbjct:  258 FQAWSNGMYKSVEHRVMTNPKVERFSTAYFMCPSYDAVI 296
The following BLAST results are available for this feature:
BLAST of EY731477 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 48
Match NameE-valueIdentityDescription
GAO1A_WHEAT3.794e-6068.60Gibberellin 20 oxidase 1-A OS=Triticum aestivum GN... [more]
GAO1B_WHEAT6.450e-6068.60Gibberellin 20 oxidase 1-B OS=Triticum aestivum GN... [more]
GAO1D_WHEAT8.411e-6068.60Gibberellin 20 oxidase 1-D OS=Triticum aestivum GN... [more]
GAOX2_ARATH1.299e-5777.46Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana G... [more]
GAOX1_ARATH1.084e-5678.26Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana G... [more]
GAOX1_ORYSJ2.180e-5458.89Gibberellin 20 oxidase 1 OS=Oryza sativa subsp. ja... [more]
GAOX3_ARATH4.829e-5476.81Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana G... [more]
GAOX2_ORYSJ3.584e-4859.52Gibberellin 20 oxidase 2 OS=Oryza sativa subsp. ja... [more]
GAOX2_ORYSI3.584e-4859.52Gibberellin 20 oxidase 2 OS=Oryza sativa subsp. in... [more]
G2OX8_ARATH4.297e-2154.84Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis th... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-704-043-H01-CT.F Sweet orange fruit, development stadium (5 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY731477 ID=EY731477; Name=EY731477; organism=Citrus sinensis; type=EST; length=834bp
CACGCGTCCGGAATCTTCCAAGGACATGATTCGATAATGAGATTGAATTA
CTACCCACCGTGCCAAAAACCTAACTTGACTCTAGGAACTGGACCCCACT
GTGACCCAACATCGTTAACAATTCTTCATCAAGATCAAGTGGGTGGCCTT
CAAGTGTATGTGGACGAACAATGGCAATCTGTTAGCCCTAACCCAAACGC
TTTTGTTGTCAACATTGGCGAATACATTCATGGCTCTATCAAATGGCATT
TACAAGAATTGCTTGCACAGAACGGCGGTTAATAATACAACTGTTAGGAA
ATCACTTGCTTTCTTTTTATGTCCAAAGATGGACAAGGTGGTGACACCTC
CGAACAACTTGGTCAATGCTAACAATCCAAGGATATATCCCGACTTCACA
TGGCACAATTTGCTCCATTTTACACAAAACTATTTTCGAGCCGACGTGAA
AACCCTCAACGCATTCTCTGCTTGGCTTCAACAGCATGTCTATATTTAAG
AGGGTGAATAGGAAATTTAAGATGATGATGACGATCTCGAGTTAATGGAA
GAAGTNTTTTNTTTTTTTTAAATTTTTTATCTTCTGTNCTTGTGGTGGAA
GGGAGAGAGGAATTTATTAAGCAAAAGGTGGCTAGCTTCTAGATTGGGAC
TACCACAAGGGGCGGCAGAGACACCCAAAAAAAGGCGCAGTTCTGTAATT
AGCTAATAACCGGTTAACGTTGAAGGGAATATTATTATATTTTGTTGGTG
GAAATCCCCGGAGCAAAAGTCAAAGAAAAAAGTACGATGGGAGTCTAACT
TAAGTACTAACTTGGGGGGGGGGGGAACCCCCCT
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