EY728939

Overview
NameEY728939
Unique NameEY728939
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length860
Libraries
Library NameType
Sweet orange fruit, development stadium (5 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of EY728939 vs. ExPASy Swiss-Prot
Match: MCD4_ASPFU (GPI ethanolamine phosphate transferase 1 OS=Aspergillus fumigatus GN=mcd4 PE=2 SV=1)

HSP 1 Score: 157.147 bits (396), Expect = 1.134e-37
Identity = 93/227 (40.97%), Postives = 124/227 (54.63%), Query Frame = 1
Query:   25 GSHGDGHPTNTDTPLVVWGAGVQHPKPISETNHSNCGFLFIDEHA---HDMPTPSEWGLNGIERVDVNQADIAPLMSTLLGLPCPVNSVGNLPLEYINMXXXXXXXXXXXXTKQILNQFLRKSHIKQTNSFYFKPFKPLVHY-----SSILDRIEGLISLRDYEMAMKLSENLRSLALQGLHYFQTYDWLMLMSVITLGYIGWMISLL-----LHVLRSYTSLSRDI 666
            GSHGDGHP NT TPLVVWG+GV  PK             + D  A   H+    ++WGL+GI+R DV QAD+A LM+ L GL  PVNSVG LPLEYI+             T+++L  +  K   K+     +KPF+PL  +        +  I+ LI+   Y+  +K S  L + AL+GL Y QTYDWL L ++IT GY+GW+   L     LHVL   +   R +
Sbjct:  289 GSHGDGHPDNTRTPLVVWGSGVAGPK-------------YTDGKAVTGHEDGFSADWGLDGIQRHDVAQADVAALMAYLAGLDFPVNSVGQLPLEYIDASPKEKALAALANTQEVLEMYRVKEAQKRAALLRYKPFEPLADHGKNSVEEHIAEIQVLIANGSYDEPIKRSGALFATALEGLRYLQTYDWLFLRTIITFGYLGWIAYALTTVIDLHVLHRTSDSKRTV 502          
BLAST of EY728939 vs. ExPASy Swiss-Prot
Match: MCD4_ASPOR (GPI ethanolamine phosphate transferase 1 OS=Aspergillus oryzae GN=mcd4 PE=3 SV=1)

HSP 1 Score: 154.836 bits (390), Expect = 5.627e-37
Identity = 86/222 (38.74%), Postives = 122/222 (54.95%), Query Frame = 1
Query:   25 GSHGDGHPTNTDTPLVVWGAGVQHPKPISETNHSNCGFLFIDEHAHDMPTPSEWGLNGIERVDVNQADIAPLMSTLLGLPCPVNSVGNLPLEYINMXXXXXXXXXXXXTKQILNQFLRKSHIKQTNSFYFKPFKPLVHYSSI-----LDRIEGLISLRDYEMAMKLSENLRSLALQGLHYFQTYDWLMLMSVITLGYIGWMISLL-----LHVLRSYTSLSR 660
            GSHGDGHP NT TPLVVWG+GV  P+   E   +           H+    ++WGL+ ++R DV QAD+A LM+ L+GL  P NSVG LPL Y++              + +L  +  K   K+     + PF+PL           L+RI+ LIS + Y+ +++LS  L   AL+GL Y QTYDWL L ++++LGY+GW+   L     LHVL   +  +R
Sbjct:  287 GSHGDGHPDNTRTPLVVWGSGVASPRYTHEGTITG----------HEDGVSADWGLDSVQRNDVAQADVAALMAYLVGLDFPTNSVGQLPLGYLDTSPKDKALAALANAQGVLEMYRVKEEQKRDALLRYTPFEPLADNGETSVEARLERIKTLISNKSYDASIQLSSELLLTALEGLRYLQTYDWLFLRTIVSLGYLGWIAYALTTVIDLHVLHGKSESNR 498          
BLAST of EY728939 vs. ExPASy Swiss-Prot
Match: MCD4_EMENI (GPI ethanolamine phosphate transferase 1 OS=Emericella nidulans GN=mcd4 PE=3 SV=1)

HSP 1 Score: 150.984 bits (380), Expect = 8.126e-36
Identity = 88/222 (39.64%), Postives = 121/222 (54.50%), Query Frame = 1
Query:   25 GSHGDGHPTNTDTPLVVWGAGVQHPKPISETNHSNCGFLFIDEHAHDMPTPSEWGLNGIERVDVNQADIAPLMSTLLGLPCPVNSVGNLPLEYINMXXXXXXXXXXXXTKQILNQFLRKSHIKQTNSFYFKPFKPLV-HYSSILDR----IEGLISLRDYEMAMKLSENLRSLALQGLHYFQTYDWLMLMSVITLGYIGWMISLL-----LHVLRSYTSLSR 660
            GSHGDGHP NT TPLVVWG+GV  PK       S           H+    ++W LN ++R DV QAD+A LM+ L+GL  P NSVG LPLEY++             T+++L  +  K   K+     ++PF+PL   Y +  ++    I+ LI    YE A++ S  L + AL+GL Y QTYDWL L +++T GY+GW+   L     LHVL   +   R
Sbjct:  287 GSHGDGHPDNTRTPLVVWGSGVAPPKQPQHGVPSG----------HEDGVSADWHLNQVQRNDVAQADVAALMAYLVGLDFPTNSVGQLPLEYVDGTPREKALAALANTQEVLEMYHVKEEHKKAALLRYRPFEPLASDYGNSAEQRLAMIKDLIDRGFYEDAIETSAALFATALEGLRYLQTYDWLFLRTIVTFGYVGWIAYALTTVIHLHVLHGASESDR 498          
BLAST of EY728939 vs. ExPASy Swiss-Prot
Match: MCD4_DEBHA (GPI ethanolamine phosphate transferase 1 OS=Debaryomyces hansenii GN=MCD4 PE=3 SV=2)

HSP 1 Score: 149.443 bits (376), Expect = 2.364e-35
Identity = 88/232 (37.93%), Postives = 126/232 (54.31%), Query Frame = 1
Query:   25 GSHGDGHPTNTDTPLVVWGAGVQHPKPISETNHSNCGFLFIDEHA--HDMPTPSEWGLNGIERVDVNQADIAPLMSTLLGLPCPVNSVGNLPLEYINMXXXXXXXXXXXXTKQILNQFLRKSHIKQTNSFYFKPFKP-----LVHYSSILDRIEGLI---SLRDYEMAMK------LSENLRSLALQGLHYFQTYDWLMLMSVITLGYIGWMISLLLHVLRSYTSLSRDILQ 672
            GSHGDGHP NT TPL+ WGAG+  PK + +T  +       D  A  ++      W L+ + R DVNQADIA LM+ L+G   P NSVG LPL+YIN             +  I+ Q+L K      + F FKP+ P     +  Y + ++ +  L+    LR+Y+  +K      LSE L   AL GL+Y QTY+W +L S++TLG+ GW+I   +  L+ +   +R IL+
Sbjct:  278 GSHGDGHPDNTRTPLIAWGAGINKPKLLRDTPDAALLSTPQDPVASGYEANYFDTWKLDHLVRNDVNQADIASLMAYLIGANYPANSVGELPLDYINAESITKIKALYANSLAIVEQYLVKESEVYNHQFSFKPYDPFKQRSIAEYKTQIETLITLLEYGDLREYDQKLKEAAAVDLSEELSKHALNGLNYLQTYNWALLRSIVTLGFFGWIIYSFIIFLKLFILTNRSILE 509          
BLAST of EY728939 vs. ExPASy Swiss-Prot
Match: PIGN_HUMAN (GPI ethanolamine phosphate transferase 1 OS=Homo sapiens GN=PIGN PE=2 SV=1)

HSP 1 Score: 141.354 bits (355), Expect = 6.438e-33
Identity = 83/218 (38.07%), Postives = 122/218 (55.96%), Query Frame = 1
Query:   25 GSHGDGHPTNTDTPLVVWGAGVQHPKPISETNHSNCGFLFIDEHAHDMPTPSEWGLNGIERVDVNQADIAPLMSTLLGLPCPVNSVGNLPLEYINMXXXXXXXXXXXXTKQILNQFLRKSHIKQ--TNSFYFKPFKPLVHYS--SILDRIEGLISLRDYEMAMKLSENLRSLALQGLHYFQTYDWLMLMSVITLGYIGWMISLLLHVLRSYTSLSRDI 666
            GSHG GHP+ T TPLV WGAG+++P+ +S     +  FL             EW L   +R+DVNQADIAPLM++L+G+P P+NSVG LP++Y+N               QIL QF  K   K+  T  F F PFK L      +IL +    I  R ++  + L + L  LAL+GL Y+ TYD   L   + +G++GW+    L +++S+++L + +
Sbjct:  269 GSHGAGHPSETLTPLVTWGAGIKYPQRVSAQQFDDA-FL------------KEWRLENWKRLDVNQADIAPLMTSLIGVPFPLNSVGILPVDYLNNTDLFKAESMFTNAVQILEQFKVKMTQKKEVTLPFLFTPFKLLSDSKQFNILRKARSYIKHRKFDEVVSLCKELIHLALKGLSYYHTYDRFFLGVNVVIGFVGWISYASLLIIKSHSNLIKGV 473          
BLAST of EY728939 vs. ExPASy Swiss-Prot
Match: MCD4_NEUCR (GPI ethanolamine phosphate transferase 1 OS=Neurospora crassa GN=mcd-4 PE=3 SV=1)

HSP 1 Score: 139.813 bits (351), Expect = 1.873e-32
Identity = 89/231 (38.53%), Postives = 119/231 (51.52%), Query Frame = 1
Query:   25 GSHGDGHPTNTDTPLVVWGAGVQHPKPISETNHSNCGFLFIDEHA--HDMPTPSEWGLNGIERVDVNQADIAPLMSTLLGLPCPVNSVGNLPLEYINMXXXXXXXXXXXXTKQILNQFLRKSHIKQTNSFYFKPFKPLVHYSSILDR----IEGLISLRDYEMAMKLSENLRSLALQGLHYFQTYDWLMLMSVITLGYIGWMISLL-----LHVLRSYTSLSRDILQGPAF 684
            GSHGDGHP NT TPL+VWG+GV  P+            L+  E A  HD  + ++W L+ + R DV QAD+A LMS L+G+  P NSVG LPL Y+               + IL Q+  K   K+     ++P++P        +R    I  LI    YE A++ S  L  + L GL Y QTYDWL L ++IT+GY+GW+   L     LHVL      SR +  G  F
Sbjct:  286 GSHGDGHPDNTRTPLIVWGSGVAKPQ------------LYPGEVAPGHDEYS-ADWNLDHVRRHDVAQADVAALMSYLVGVEFPANSVGELPLSYLAADIKEKAEASLVNVQGILEQYRVKEEKKKATELKYRPYQPFGENGLSPERRVAEIRQLIDAGRYEEAIEESAALMKVGLGGLRYLQTYDWLFLRALITIGYLGWIAYALTTVVDLHVLHGRVRPSRTLGGGLFF 503          
BLAST of EY728939 vs. ExPASy Swiss-Prot
Match: MCD4_GIBZE (GPI ethanolamine phosphate transferase 1 OS=Gibberella zeae GN=MCD4 PE=3 SV=1)

HSP 1 Score: 139.813 bits (351), Expect = 1.873e-32
Identity = 88/233 (37.77%), Postives = 120/233 (51.50%), Query Frame = 1
Query:   25 GSHGDGHPTNTDTPLVVWGAGVQ----HPKPISETNHSNCGFLFIDEHAHDMPTPSEWGLNGIERVDVNQADIAPLMSTLLGLPCPVNSVGNLPLEYINMXXXXXXXXXXXXTKQILNQFLRKSHIKQTNSFYFKPFKPL----VHYSSILDRIEGLISLRDYEMAMKLSENLRSLALQGLHYFQTYDWLMLMSVITLGYIGWM-----ISLLLHVLRSYTSLSRDILQGPAF 684
            GSHGDGHP NT TPL+ WG+GV     HP  I+                HD    S+W L+ + R DV QAD+A LMS L+G   P N VG LPL +++              + IL Q+  K   K+     ++P+  L    +   S +  I  LI    +E A++ S+ L S+ LQGL Y QTYDWL L ++IT+GY+GWM       L L+V++   S  R +L G AF
Sbjct:  287 GSHGDGHPNNTRTPLISWGSGVAAPELHPGSIAP--------------GHD-ELSSDWNLDHVRRHDVAQADVAALMSYLIGTEFPANGVGQLPLNFLSASIKEKAEASLANAQVILEQYRVKEENKRNVELRYQPYGQLSDGNLDPESRISHIRSLIEAGSFEEAIEESDALMSIGLQGLRYLQTYDWLFLRALITIGYLGWMAYATTTVLSLYVVKESMSPQRTLL-GSAF 503          
BLAST of EY728939 vs. ExPASy Swiss-Prot
Match: MCD4_CHAGB (GPI ethanolamine phosphate transferase 1 OS=Chaetomium globosum GN=MCD4 PE=3 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 5.450e-32
Identity = 86/226 (38.05%), Postives = 117/226 (51.77%), Query Frame = 1
Query:   25 GSHGDGHPTNTDTPLVVWGAGVQHPKPISETNHSNCGFLFIDEHA--HDMPTPSEWGLNGIERVDVNQADIAPLMSTLLGLPCPVNSVGNLPLEYINMXXXXXXXXXXXXTKQILNQFLRKSHIKQTNSFYFKPFKPL----VHYSSILDRIEGLISLRDYEMAMKLSENLRSLALQGLHYFQTYDWLMLMSVITLGYIGWMISLL-----LHVLRSYTSLSRDIL 669
            G+HGDGHP NT TPL+ WG+GV  P+            L+  E A  HD  + S+W L+ I R DV QAD+A LM+ L G   P NSVG LPL ++               + IL Q+  K   K+     ++P++PL    +   S +  I  LI    YE A++ S  L  + L GL Y QTYDWL L ++IT+GY+GW+   L     LHVL      SR ++
Sbjct:  208 GNHGDGHPDNTRTPLIAWGSGVAKPQ------------LYPGEVAPGHDEYS-SDWNLDHIRRHDVEQADVAALMAYLAGTEFPANSVGELPLSFLTAGLKEKAEASLVNAQGILEQYRVKEEGKKLTELRYRPYEPLSDEGMATESRVAHIRQLIETGSYEEAIEESAALLKVGLGGLRYLQTYDWLFLRALITIGYLGWVAYALTTVIDLHVLHGRIQPSRTLI 420          
BLAST of EY728939 vs. ExPASy Swiss-Prot
Match: MCD4_SCHPO (GPI ethanolamine phosphate transferase 1 OS=Schizosaccharomyces pombe GN=its8 PE=1 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 7.118e-32
Identity = 77/200 (38.50%), Postives = 112/200 (56.00%), Query Frame = 1
Query:   25 GSHGDGHPTNTDTPLVVWGAGVQHPKPISETNHSNCGFLFIDEHAHDMPTPSEWGLNGIERVDVNQADIAPLMSTLLGLPCPVNSVGNLPLEYINMXXXXXXXXXXXXTKQILNQFLRKSHIKQTNSFYFKPFKPLVHY----SSILDRIEGLISLRDYEMAMKLSENLRSLALQGLHYFQTYDWLMLMSVITLGYIGWM 612
            GSHGDG+  NT TP++ WGAG+Q P     T+  N G    DE++  +P    W L  I+R+D+ QADIA LMS L+GL  PVNSVG +PL+Y++               +I  Q+  KS  K   S +F+P+ PL +Y    +S  + +   I   +YE+A++   +     L GL Y Q YDWL+L S++  GY+ W+
Sbjct:  275 GSHGDGNLDNTRTPIIAWGAGIQSP-----THEKNYGH---DEYS--LP----WNLTEIKRIDIQQADIAALMSYLVGLNFPVNSVGQIPLDYLDCSSRRKAEVALMNALEIGEQYNLKSASKDQTSIFFRPYSPLRNYTEVQASFYNSVIADIESGEYEIAIEHCFHFSQTVLSGLRYLQRYDWLLLRSIVFFGYLSWI 460          
BLAST of EY728939 vs. ExPASy Swiss-Prot
Match: PIGN_MOUSE (GPI ethanolamine phosphate transferase 1 OS=Mus musculus GN=Pign PE=2 SV=2)

HSP 1 Score: 137.502 bits (345), Expect = 9.297e-32
Identity = 83/216 (38.43%), Postives = 117/216 (54.17%), Query Frame = 1
Query:   25 GSHGDGHPTNTDTPLVVWGAGVQHPKPISETNHSNCGFLFIDEHAHDMPTPSEWGLNGIERVDVNQADIAPLMSTLLGLPCPVNSVGNLPLEYINMXXXXXXXXXXXXTKQILNQFLRKSHIKQ--TNSFYFKPFKPL--VHYSSILDRIEGLISLRDYEMAMKLSENLRSLALQGLHYFQTYDWLMLMSVITLGYIGWMISLLLHVLRSYTSLSR 660
            GSHG GHP+ T TP V WGAG++ P+ +S   + +  FL             EW L   +R DVNQADIAPLM++L+G+P P+NSVG LP+ Y+N               QIL QF  K   K+  T  F F PFK L       IL +    I    ++  + L E L  LAL+GL Y+ TYD L L   + +G++GWM    L +++S++++ +
Sbjct:  269 GSHGAGHPSETLTPFVTWGAGIKFPQNVSAQQYDD-EFL------------KEWRLENWKRRDVNQADIAPLMASLIGVPFPLNSVGILPVGYLNNTGLFKAESMFTNAVQILEQFKVKMTQKKEATLPFLFTPFKLLSDSQQLDILRKARSYIKQEKFDEVVSLCEELIDLALRGLSYYHTYDRLFLGINVAVGFVGWMSYTSLLIIKSHSNIPK 471          
The following BLAST results are available for this feature:
BLAST of EY728939 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 16
Match NameE-valueIdentityDescription
MCD4_ASPFU1.134e-3740.97GPI ethanolamine phosphate transferase 1 OS=Asperg... [more]
MCD4_ASPOR5.627e-3738.74GPI ethanolamine phosphate transferase 1 OS=Asperg... [more]
MCD4_EMENI8.126e-3639.64GPI ethanolamine phosphate transferase 1 OS=Emeric... [more]
MCD4_DEBHA2.364e-3537.93GPI ethanolamine phosphate transferase 1 OS=Debary... [more]
PIGN_HUMAN6.438e-3338.07GPI ethanolamine phosphate transferase 1 OS=Homo s... [more]
MCD4_NEUCR1.873e-3238.53GPI ethanolamine phosphate transferase 1 OS=Neuros... [more]
MCD4_GIBZE1.873e-3237.77GPI ethanolamine phosphate transferase 1 OS=Gibber... [more]
MCD4_CHAGB5.450e-3238.05GPI ethanolamine phosphate transferase 1 OS=Chaeto... [more]
MCD4_SCHPO7.118e-3238.50GPI ethanolamine phosphate transferase 1 OS=Schizo... [more]
PIGN_MOUSE9.297e-3238.43GPI ethanolamine phosphate transferase 1 OS=Mus mu... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-704-010-E11-CT.F Sweet orange fruit, development stadium (5 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY728939 ID=EY728939; Name=EY728939; organism=Citrus sinensis; type=EST; length=860bp
CACGCGTCCGGGGATGAGTGACAAGGTAGTCATGGAGATGGGCATCCTAC
AAACACTGACACCCCCCTTGTTGTTTGGGGAGCTGGCGTTCAACATCCTA
AGCCAATCTCTGAAACGAATCATTCTAATTGTGGTTTTCTTTTCATCGAT
GAACATGCACATGACATGCCAACCCCTAGTGAATGGGGCCTTAATGGCAT
TGAAAGGGTAGATGTCAATCAAGCTGATATCGCACCACTCATGTCAACTC
TTCTTGGTCTGCCATGTCCTGTGAACTCTGTTGGAAATCTTCCACTTGAA
TACATTAACATGAATGAGGCAGAAAATGCTGAAGCTTTGCTAGCCAATAC
AAAGCAAATTCTCAATCAGTTTCTTCGGAAATCACATATAAAGCAGACAA
ACTCATTTTATTTCAAGCCTTTTAAGCCGCTAGTTCATTACTCTTCTATA
TTGGACCGGATTGAGGGTCTAATATCTCTTAGAGACTATGAAATGGCAAT
GAAGCTTTCAGAAAATTTGAGAAGCCTGGCACTGCAAGGGCTTCATTATT
TTCAAACATACGACTGGTTGATGCTGATGTCAGTTATTACTCTTGGGTAT
ATTGGTTGGATGATCAGTCTTCTTCTCCATGTGCTGCGATCTTATACTTC
ATTATCGAGAGATATATTACAGGGGCCAGCATTCCACCAGGGAAAACATA
CCAGAAAAGTATACTATTCGGATGTCTGTTGATGGGAGTAGTTCTATANA
ATTATTCTGGAGCACTCTCCTCCATTACCATGCCTACATTGCAATGACGT
GTTTCTCTGGACCAAATACTAGTGAATATCATTTGTACTACCATTGTGGA
GACCCTTTGT
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