EY728933 | Citrus Genome Database

Overview


Name	EY728933
Unique Name	EY728933
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	858

Libraries


Library Name	Type
Sweet orange fruit, development stadium (5 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY728933 vs. ExPASy Swiss-Prot
Match: HSP7M_PHAVU (Heat shock 70 kDa protein, mitochondrial OS=Phaseolus vulgaris PE=2 SV=1)

HSP 1 Score: 246.128 bits (627), Expect = 1.108e-80
Identity = 121/149 (81.21%), Postives = 134/149 (89.93%), Query Frame = 1

Query:  112 IAGILRSLRRRDVASAHLSACKSLTCNVKPSWASANWASLARPFSVRPAGNDVIGVDLGTTNSCVAVMEGKNPKVIENSEGTRTTPSVVAFNQKGELLVGTPAKRQAVTNPANTLFGTKRLIGRRFNDAQTQKEMKMVPFKIVNAPNGE 558
            +A +LRSLRRRDVASA  SA +SLT + KP++ +  W+ LARPFS RPAGNDVIG+DLGTTNSCV+VMEGKNPKVIENSEG RTTPSVVAFNQKGELLVGTPAKRQAVTNP NT+FGTKRLIGRRF+D QTQKEMKMVPFKIV APNG+
Sbjct:    1 MAAVLRSLRRRDVASATFSAYRSLTGSTKPAYVAQKWSCLARPFSSRPAGNDVIGIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNTVFGTKRLIGRRFDDPQTQKEMKMVPFKIVKAPNGD 149

HSP 2 Score: 76.2554 bits (186), Expect = 1.108e-80
Identity = 51/100 (51.00%), Postives = 60/100 (60.00%), Query Frame = 2

Query:  557 NAWVEANGQQYSPSQIGAFVLTKMKETAESYLXKVCF*SSDHCTSLX***SQEAXQRRMLGKIGRSPMFQKIINEPTGCWPFL*GVEKGEGPLAFFDLGG 856
            +AWVEANGQQYSPSQIGAFVLTKMKETAE+YL K    S    T        +    +  G+I    + Q+IINEPT       G+   EG +A FDLGG
Sbjct:  149 DAWVEANGQQYSPSQIGAFVLTKMKETAEAYLGKSV--SKAVITVPAYFNDAQRQATKDAGRIAGLDV-QRIINEPTAA-ALSYGMNNKEGLIAVFDLGG 244

[Close]

BLAST of EY728933 vs. ExPASy Swiss-Prot
Match: HSP7M_PEA (Heat shock 70 kDa protein, mitochondrial OS=Pisum sativum GN=HSP1 PE=2 SV=1)

HSP 1 Score: 233.802 bits (595), Expect = 9.275e-77
Identity = 117/148 (79.05%), Postives = 133/148 (89.86%), Query Frame = 1

Query:  115 AGILRSLRRRDVASAHLSACKSLTCNVKPSWASANWASLARPFSVRPAGNDVIGVDLGTTNSCVAVMEGKNPKVIENSEGTRTTPSVVAFNQKGELLVGTPAKRQAVTNPANTLFGTKRLIGRRFNDAQTQKEMKMVPFKIVNAPNGE 558
            A +LRSL+RR+++S+ +SA +SLT + K S+A+   ASL RPFS RPAGNDVIG+DLGTTNSCV+VMEGKNPKVIENSEG RTTPSVVAFNQK ELLVGTPAKRQAVTNP NTLFGTKRLIGRRF+DAQTQKEMKMVP+KIV APNG+
Sbjct:    3 ATLLRSLQRRNLSSSSVSAFRSLTGSTKTSYATHKLASLTRPFSSRPAGNDVIGIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKSELLVGTPAKRQAVTNPTNTLFGTKRLIGRRFDDAQTQKEMKMVPYKIVRAPNGD 150

HSP 2 Score: 75.485 bits (184), Expect = 9.275e-77
Identity = 50/100 (50.00%), Postives = 60/100 (60.00%), Query Frame = 2

Query:  557 NAWVEANGQQYSPSQIGAFVLTKMKETAESYLXKVCF*SSDHCTSLX***SQEAXQRRMLGKIGRSPMFQKIINEPTGCWPFL*GVEKGEGPLAFFDLGG 856
            +AWVEANGQQYSPSQIGAFVLTK+KETAE+YL K    S    T        +    +  G+I    + Q+IINEPT       G+   EG +A FDLGG
Sbjct:  150 DAWVEANGQQYSPSQIGAFVLTKIKETAEAYLGKTI--SKAVVTVPAYFNDAQRQATKDAGRIAGLDV-QRIINEPTAA-ALSYGMNNKEGLIAVFDLGG 245

[Close]

BLAST of EY728933 vs. ExPASy Swiss-Prot
Match: HSP7M_SOLTU (Heat shock 70 kDa protein, mitochondrial OS=Solanum tuberosum GN=HSP68 PE=2 SV=1)

HSP 1 Score: 235.728 bits (600), Expect = 1.210e-76
Identity = 117/155 (75.48%), Postives = 131/155 (84.52%), Query Frame = 1

Query:  106 MAIAGILRSLRRRDVASAHLSACKSLTCNVKPSWA----SANWASLARPFSVRPAGNDVIGVDLGTTNSCVAVMEGKNPKVIENSEGTRTTPSVVAFNQKGELLVGTPAKRQAVTNPANTLFGTKRLIGRRFNDAQTQKEMKMVPFKIVNAPNGE 558
            MA A +LRSLRRR+ A++ +SA ++L  N KPSW      A WA LARPFS +PAGN++IG+DLGTTNSCVAVMEGKNPKVIENSEG RTTPSVVAFNQKGELLVGTPAKRQAVTNP NTL GTKRLIGRRF+D QTQKEMKMVP+KIV   NG+
Sbjct:    1 MATAALLRSLRRREFATSSISAYRTLASNTKPSWCPSLVGAKWAGLARPFSSKPAGNEIIGIDLGTTNSCVAVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNTLSGTKRLIGRRFDDPQTQKEMKMVPYKIVRGSNGD 155

HSP 2 Score: 73.1738 bits (178), Expect = 1.210e-76
Identity = 50/102 (49.02%), Postives = 61/102 (59.80%), Query Frame = 2

Query:  557 NAWVEANGQQYSPSQIGAFVLTKMKETAESYLXKVCF*SSDHCTSLX***SQEAXQRRMLGKIGRSPMF--QKIINEPTGCWPFL*GVEKGEGPLAFFDLGG 856
            +AWVEANGQQYSP+QIGAF+LTKMKETAE+YL K    S +           +A QR+ +   G       Q+IINEPT       G+   EG +A FDLGG
Sbjct:  155 DAWVEANGQQYSPTQIGAFILTKMKETAEAYLGK----SINKAVITVPAYFNDA-QRQAIKDAGAIAGLDVQRIINEPTAA-ALSYGMNSKEGLVAVFDLGG 250

[Close]

BLAST of EY728933 vs. ExPASy Swiss-Prot
Match: GRP75_CHICK (Stress-70 protein, mitochondrial OS=Gallus gallus GN=HSPA9 PE=1 SV=1)

HSP 1 Score: 149.828 bits (377), Expect = 1.865e-46
Identity = 74/112 (66.07%), Postives = 88/112 (78.57%), Query Frame = 1

Query:  223 ASLARPFSVRPAGNDVIGVDLGTTNSCVAVMEGKNPKVIENSEGTRTTPSVVAFNQKGELLVGTPAKRQAVTNPANTLFGTKRLIGRRFNDAQTQKEMKMVPFKIVNAPNGE 558
            A+ +R ++       VIG+DLGTTNSCVAVMEGK  KV+ENSEG RTTPSVVAF   GE LVG PAKRQAVTNP NT + TKRLIGRRF+D++ +K++K VPFKIV A NG+
Sbjct:   42 AASSRKYASEAIKGAVIGIDLGTTNSCVAVMEGKQAKVLENSEGARTTPSVVAFTADGERLVGMPAKRQAVTNPHNTFYATKRLIGRRFDDSEVKKDIKNVPFKIVRASNGD 153

HSP 2 Score: 58.151 bits (139), Expect = 1.865e-46
Identity = 42/101 (41.58%), Postives = 57/101 (56.44%), Query Frame = 2

Query:  557 NAWVEANGQQYSPSQIGAFVLTKMKETAESYLXKVCF*SSDHCTSLX***SQEAXQRRMLGKIGRSPMFQKIINEPTGCWPFL*GVEKGEGP-LAFFDLGG 856
            +AWVEA+G+ YSPSQIGAFVL KMKETAE+YL       +   T        +    +  G+I    +  ++INEPT       G++K E   +A +DLGG
Sbjct:  153 DAWVEAHGKLYSPSQIGAFVLMKMKETAENYLGHPA--KNAVITVPAYFNDSQRQATKDAGQISGLNVL-RVINEPTAA-ALAYGLDKSEDKIIAVYDLGG 249

[Close]

BLAST of EY728933 vs. ExPASy Swiss-Prot
Match: DNAK_PARL1 (Chaperone protein dnaK OS=Parvibaculum lavamentivorans (strain DS-1 / DSM 13023 / NCIMB 13966) GN=dnaK PE=2 SV=1)

HSP 1 Score: 143.28 bits (360), Expect = 7.014e-46
Identity = 71/97 (73.20%), Postives = 79/97 (81.44%), Query Frame = 1

Query:  268 VIGVDLGTTNSCVAVMEGKNPKVIENSEGTRTTPSVVAFNQKGELLVGTPAKRQAVTNPANTLFGTKRLIGRRFNDAQTQKEMKMVPFKIVNAPNGE 558
            VIG+DLGTTNSCVAVMEG + KVIENSEG RTTPS+VAF Q GE LVG  AKRQAVTNP NTLF  KRLIGR ++D  TQK+  MVP+ IV APNG+
Sbjct:    4 VIGIDLGTTNSCVAVMEGSSAKVIENSEGMRTTPSMVAFTQDGERLVGQSAKRQAVTNPTNTLFAIKRLIGRSYDDPTTQKDKGMVPYDIVKAPNGD 100

HSP 2 Score: 62.7734 bits (151), Expect = 7.014e-46
Identity = 45/101 (44.55%), Postives = 58/101 (57.43%), Query Frame = 2

Query:  557 NAWVEANGQQYSPSQIGAFVLTKMKETAESYLXKVCF*SSDHCTSLX***SQEAXQRRMLGKIGRSPMFQKIINEPTGCWPFL*GVEKGEGPL-AFFDLGG 856
            +AWVEA+G++YSPSQI AF+L KMKETAESYL +         T        +    +  GKI    +  +IINEPT       G++K EG L A +DLGG
Sbjct:  100 DAWVEAHGEKYSPSQISAFILQKMKETAESYLGEKV--EKAVITVPAYFNDAQRQATKDAGKIAGLEVL-RIINEPTAA-ALAYGLDKKEGELIAVYDLGG 196

[Close]

BLAST of EY728933 vs. ExPASy Swiss-Prot
Match: GRP75_PONAB (Stress-70 protein, mitochondrial OS=Pongo abelii GN=HSPA9 PE=2 SV=1)

HSP 1 Score: 146.362 bits (368), Expect = 1.191e-45
Identity = 70/97 (72.16%), Postives = 81/97 (83.51%), Query Frame = 1

Query:  268 VIGVDLGTTNSCVAVMEGKNPKVIENSEGTRTTPSVVAFNQKGELLVGTPAKRQAVTNPANTLFGTKRLIGRRFNDAQTQKEMKMVPFKIVNAPNGE 558
            V+G+DLGTTNSCVAVMEGK  KV+EN+EG RTTPSVVAF   GE LVG PAKRQAVTNP NT + TKRLIGRR++D + QK++K VPFKIV A NG+
Sbjct:   55 VVGIDLGTTNSCVAVMEGKQAKVLENAEGARTTPSVVAFTADGERLVGMPAKRQAVTNPNNTFYATKRLIGRRYDDPEVQKDIKNVPFKIVRASNGD 151

HSP 2 Score: 58.9214 bits (141), Expect = 1.191e-45
Identity = 42/101 (41.58%), Postives = 57/101 (56.44%), Query Frame = 2

Query:  557 NAWVEANGQQYSPSQIGAFVLTKMKETAESYLXKVCF*SSDHCTSLX***SQEAXQRRMLGKIGRSPMFQKIINEPTGCWPFL*GVEKGEGP-LAFFDLGG 856
            +AWVEA+G+ YSPSQIGAFVL KMKETAE+YL       +   T        +    +  G+I    +  ++INEPT       G++K E   +A +DLGG
Sbjct:  151 DAWVEAHGKLYSPSQIGAFVLMKMKETAENYLGHTA--KNAVITVPAYFNDSQRQATKDAGQISGLNVL-RVINEPTAA-ALAYGLDKSEDKVIAVYDLGG 247

[Close]

BLAST of EY728933 vs. ExPASy Swiss-Prot
Match: GRP75_HUMAN (Stress-70 protein, mitochondrial OS=Homo sapiens GN=HSPA9 PE=1 SV=2)

HSP 1 Score: 146.362 bits (368), Expect = 1.191e-45
Identity = 70/97 (72.16%), Postives = 81/97 (83.51%), Query Frame = 1

Query:  268 VIGVDLGTTNSCVAVMEGKNPKVIENSEGTRTTPSVVAFNQKGELLVGTPAKRQAVTNPANTLFGTKRLIGRRFNDAQTQKEMKMVPFKIVNAPNGE 558
            V+G+DLGTTNSCVAVMEGK  KV+EN+EG RTTPSVVAF   GE LVG PAKRQAVTNP NT + TKRLIGRR++D + QK++K VPFKIV A NG+
Sbjct:   55 VVGIDLGTTNSCVAVMEGKQAKVLENAEGARTTPSVVAFTADGERLVGMPAKRQAVTNPNNTFYATKRLIGRRYDDPEVQKDIKNVPFKIVRASNGD 151

HSP 2 Score: 58.9214 bits (141), Expect = 1.191e-45
Identity = 42/101 (41.58%), Postives = 57/101 (56.44%), Query Frame = 2

Query:  557 NAWVEANGQQYSPSQIGAFVLTKMKETAESYLXKVCF*SSDHCTSLX***SQEAXQRRMLGKIGRSPMFQKIINEPTGCWPFL*GVEKGEGP-LAFFDLGG 856
            +AWVEA+G+ YSPSQIGAFVL KMKETAE+YL       +   T        +    +  G+I    +  ++INEPT       G++K E   +A +DLGG
Sbjct:  151 DAWVEAHGKLYSPSQIGAFVLMKMKETAENYLGHTA--KNAVITVPAYFNDSQRQATKDAGQISGLNVL-RVINEPTAA-ALAYGLDKSEDKVIAVYDLGG 247

[Close]

BLAST of EY728933 vs. ExPASy Swiss-Prot
Match: GRP75_BOVIN (Stress-70 protein, mitochondrial OS=Bos taurus GN=HSPA9 PE=2 SV=1)

HSP 1 Score: 146.362 bits (368), Expect = 1.191e-45
Identity = 70/97 (72.16%), Postives = 81/97 (83.51%), Query Frame = 1

Query:  268 VIGVDLGTTNSCVAVMEGKNPKVIENSEGTRTTPSVVAFNQKGELLVGTPAKRQAVTNPANTLFGTKRLIGRRFNDAQTQKEMKMVPFKIVNAPNGE 558
            V+G+DLGTTNSCVAVMEGK  KV+EN+EG RTTPSVVAF   GE LVG PAKRQAVTNP NT + TKRLIGRR++D + QK++K VPFKIV A NG+
Sbjct:   55 VVGIDLGTTNSCVAVMEGKQAKVLENAEGARTTPSVVAFTADGERLVGMPAKRQAVTNPNNTFYATKRLIGRRYDDPEVQKDIKNVPFKIVRASNGD 151

HSP 2 Score: 58.9214 bits (141), Expect = 1.191e-45
Identity = 42/101 (41.58%), Postives = 57/101 (56.44%), Query Frame = 2

Query:  557 NAWVEANGQQYSPSQIGAFVLTKMKETAESYLXKVCF*SSDHCTSLX***SQEAXQRRMLGKIGRSPMFQKIINEPTGCWPFL*GVEKGEGP-LAFFDLGG 856
            +AWVEA+G+ YSPSQIGAFVL KMKETAE+YL       +   T        +    +  G+I    +  ++INEPT       G++K E   +A +DLGG
Sbjct:  151 DAWVEAHGKLYSPSQIGAFVLMKMKETAENYLGHTA--KNAVITVPAYFNDSQRQATKDAGQISGLNVL-RVINEPTAA-ALAYGLDKSEDKIIAVYDLGG 247

[Close]

BLAST of EY728933 vs. ExPASy Swiss-Prot
Match: DNAK_BEII9 (Chaperone protein dnaK OS=Beijerinckia indica subsp. indica (strain ATCC 9039 / DSM 1715 / NCIB 8712) GN=dnaK PE=2 SV=1)

HSP 1 Score: 140.969 bits (354), Expect = 1.191e-45
Identity = 67/97 (69.07%), Postives = 76/97 (78.35%), Query Frame = 1

Query:  268 VIGVDLGTTNSCVAVMEGKNPKVIENSEGTRTTPSVVAFNQKGELLVGTPAKRQAVTNPANTLFGTKRLIGRRFNDAQTQKEMKMVPFKIVNAPNGE 558
            VIG+DLGTTNSCVAVMEG  PKVIEN+EG RTTPS+VAF   GE LVG PAKRQ VTNP  T F  KRLIGR F D  T+K++ +VP+KI  APNG+
Sbjct:    4 VIGIDLGTTNSCVAVMEGTTPKVIENAEGARTTPSIVAFTDDGERLVGQPAKRQGVTNPERTFFAIKRLIGRTFEDPMTKKDIGLVPYKITKAPNGD 100

HSP 2 Score: 64.3142 bits (155), Expect = 1.191e-45
Identity = 44/100 (44.00%), Postives = 56/100 (56.00%), Query Frame = 2

Query:  557 NAWVEANGQQYSPSQIGAFVLTKMKETAESYLXKVCF*SSDHCTSLX***SQEAXQRRMLGKIGRSPMFQKIINEPTGCWPFL*GVEKGEGPLAFFDLGG 856
            +AWVEA+G+ YSPSQI AF+L KMKETAESYL +    S    T        +    +  GKI    +  +IINEPT         +KG G +A +DLGG
Sbjct:  100 DAWVEADGKPYSPSQISAFILQKMKETAESYLGQPV--SQAVITVPAYFNDAQRQATKDAGKIAGLEVL-RIINEPTAAALAYGLDKKGSGTIAVYDLGG 196

[Close]

BLAST of EY728933 vs. ExPASy Swiss-Prot
Match: DNAK_METSB (Chaperone protein dnaK OS=Methylocella silvestris (strain BL2 / DSM 15510 / NCIMB 13906) GN=dnaK PE=2 SV=1)

HSP 1 Score: 140.198 bits (352), Expect = 1.553e-45
Identity = 66/97 (68.04%), Postives = 78/97 (80.41%), Query Frame = 1

Query:  268 VIGVDLGTTNSCVAVMEGKNPKVIENSEGTRTTPSVVAFNQKGELLVGTPAKRQAVTNPANTLFGTKRLIGRRFNDAQTQKEMKMVPFKIVNAPNGE 558
            VIG+DLGTTNSCVAVMEG  PKVIEN+EG RTTPS+VAF   GE LVG PAKRQ+VTNP  T F  KRLIGR ++D  T+K+M +VP+KI+ A NG+
Sbjct:    4 VIGIDLGTTNSCVAVMEGTTPKVIENAEGARTTPSIVAFTDDGERLVGQPAKRQSVTNPERTFFAIKRLIGRTYDDPMTKKDMGLVPYKIIRASNGD 100

HSP 2 Score: 64.6994 bits (156), Expect = 1.553e-45
Identity = 44/100 (44.00%), Postives = 57/100 (57.00%), Query Frame = 2

Query:  557 NAWVEANGQQYSPSQIGAFVLTKMKETAESYLXKVCF*SSDHCTSLX***SQEAXQRRMLGKIGRSPMFQKIINEPTGCWPFL*GVEKGEGPLAFFDLGG 856
            +AWVEA+G+QYSPSQI AF+L KMKETAE+YL +    S    T        +    +  GKI    +  +IINEPT         +KG G +A +DLGG
Sbjct:  100 DAWVEADGKQYSPSQISAFILQKMKETAEAYLGQPV--SQAVITVPAYFNDAQRQATKDAGKIAGLEVL-RIINEPTAAALAYGLDKKGAGVIAVYDLGG 196

The following BLAST results are available for this feature:

BLAST of EY728933 vs. ExPASy Swiss-Prot

BLAST of EY728933 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
HSP7M_PHAVU	1.108e-80	81.21	Heat shock 70 kDa protein, mitochondrial OS=Phaseo...	[more]
HSP7M_PEA	9.275e-77	79.05	Heat shock 70 kDa protein, mitochondrial OS=Pisum ...	[more]
HSP7M_SOLTU	1.210e-76	75.48	Heat shock 70 kDa protein, mitochondrial OS=Solanu...	[more]
GRP75_CHICK	1.865e-46	66.07	Stress-70 protein, mitochondrial OS=Gallus gallus ...	[more]
DNAK_PARL1	7.014e-46	73.20	Chaperone protein dnaK OS=Parvibaculum lavamentivo...	[more]
GRP75_PONAB	1.191e-45	72.16	Stress-70 protein, mitochondrial OS=Pongo abelii G...	[more]
GRP75_HUMAN	1.191e-45	72.16	Stress-70 protein, mitochondrial OS=Homo sapiens G...	[more]
GRP75_BOVIN	1.191e-45	72.16	Stress-70 protein, mitochondrial OS=Bos taurus GN=...	[more]
DNAK_BEII9	1.191e-45	69.07	Chaperone protein dnaK OS=Beijerinckia indica subs...	[more]
DNAK_METSB	1.553e-45	68.04	Chaperone protein dnaK OS=Methylocella silvestris ...	[more]

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Properties


Property Name	Value
Genbank description	CS00-C3-704-010-E04-CT.F Sweet orange fruit, development stadium (5 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY728933 ID=EY728933; Name=EY728933; organism=Citrus sinensis; type=EST; length=858bp
CCACGCGTCCGAACCCTAAACCCTTGGAAAAGCAACCTAAAACGCTACAA
GCTTTCTCTCGCTTTCAAAAGAGCTAAAGCAAATCGATTAGTCGATATTT
ACTCAATGGCCATCGCCGGTATTCTCCGCTCCTTACGACGGCGAGATGTC
GCCTCCGCCCATCTCTCTGCCTGCAAATCCTTGACGTGCAATGTCAAACC
CTCCTGGGCTTCTGCAAATTGGGCGAGTTTGGCAAGACCTTTCAGTGTGA
GACCAGCAGGGAATGATGTTATTGGGGTTGATTTGGGAACCACCAATTCA
TGTGTTGCGGTCATGGAAGGAAAGAATCCTAAAGTTATTGAGAATTCTGA
AGGCACGAGGACCACGCCGTCAGTGGTGGCCTTCAATCAGAAAGGTGAGT
TGCTTGTGGGTACACCAGCAAAACGTCAGGCTGTGACTAACCCAGCTAAT
ACACTTTTTGGAACTAAGCGTCTGATTGGTAGGCGATTCAATGATGCCCA
AACTCAAAAAGAAATGAAAATGGTTCCATTCAAGATAGTCAATGCTCCAA
ACGGAGAATGCTTGGGTCGAGGCCAATGGGCAACAGTACTCCCCAAGCCA
GATCGGAGCATTCGTTCTAACCAAGATGAAAGAAACAGCAGAGTCTTATC
TTNGGAAAGTCTGTTTCTAAAGCAGTGATCACTGTACCAGCTTATNTTAA
TGATGATCTCAGGAGGCCANCCAACGAAGGATGCTTGGTAAAATTGGCCG
GTCTCCAATGTTCCAAAAAATTATAAATGAGCCTACTGGCTGCTGGCCTT
TCCTATAGGGTGTAGAAAAAGGAGAGGGTCCCCTTGCATTTTTTGATCTT
GGGGGGGG