EY754176

Overview
NameEY754176
Unique NameEY754176
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length921
Libraries
Library NameType
Sweet orange leaf, field plant Acdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY754176 vs. ExPASy Swiss-Prot
Match: AFC1_ARATH (Serine/threonine-protein kinase AFC1 OS=Arabidopsis thaliana GN=AFC1 PE=2 SV=2)

HSP 1 Score: 259.996 bits (663), Expect = 1.036e-89
Identity = 120/176 (68.18%), Postives = 141/176 (80.11%), Query Frame = 3 
Query:  186 METQRIIEFPHKNMDKRPRKRPRLTWDXXXXXXXXXXXXA-----LYCVQEFGNGGMPNYACSSMFYGAIPRTGSPPWRPDDKDGHYVFAIGENLTPRYRILSKMGEGTFGQVVECFDNEKKELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGTRCVQIRNWFDYRNHICIL 698
            +ETQR +EFPH+ +DKRPRKRPRLTWD                  LY   EF +G +PN+   +MFY  +PR GSPPWRPDDKDGHYVF +G+ LTPRY+ILSKMGEGTFGQV+ECFDN+ KE+VAIK++RSINKYREAAMIEIDVLQRL R+D+GG+RCVQIRNWFDYRNHICI+
Sbjct:   17 LETQRNVEFPHRIVDKRPRKRPRLTWDAAPPLLPPPPPPTVFQPPLYYGPEFASGLVPNFVYPNMFYNGLPRQGSPPWRPDDKDGHYVFVVGDTLTPRYQILSKMGEGTFGQVLECFDNKNKEVVAIKVIRSINKYREAAMIEIDVLQRLTRHDVGGSRCVQIRNWFDYRNHICIV 192

HSP 2 Score: 92.4337 bits (228), Expect = 1.036e-89
Identity = 41/49 (83.67%), Postives = 47/49 (95.92%), Query Frame = 2 
Query:  695 FMHELRLIHTDLKPXNILLVSAEYVKVPDYKFLSRSSKDGSYFKNLPKS 841
            +MH+LRLIHTDLKP NILLVS+EY+K+PDYKFLSR +KDGSYFKNLPKS
Sbjct:  230 YMHDLRLIHTDLKPENILLVSSEYIKIPDYKFLSRPTKDGSYFKNLPKS 278
[Close]
BLAST of EY754176 vs. ExPASy Swiss-Prot
Match: AFC2_ARATH (Serine/threonine-protein kinase AFC2 OS=Arabidopsis thaliana GN=AFC2 PE=1 SV=1)

HSP 1 Score: 224.172 bits (570), Expect = 3.279e-70
Identity = 105/177 (59.32%), Postives = 131/177 (74.01%), Query Frame = 3 
Query:  186 METQRIIEFPHKNMDKRPRKRPRLTWDXXXXXXXXXXXXALYCVQEFGN------GGMPNYACSSMFYGAIPRTGSPPWRPDDKDGHYVFAIGENLTPRYRILSKMGEGTFGQVVECFDNEKKELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGTRCVQIRNWFDYRNHICIL 698
            ME +R+ EFPH +MD+RPRKR RL WD             ++C QE GN       G P+   SS+    + R GSPPWR DDKDGHY+F +G++LTPRY+I SKMGEGTFGQV+EC+D E+KE+VA+KIVR + KYREAAMIEI++LQ+L ++D GG RCVQIRNWFDYRNHICI+
Sbjct:    1 MEMERVHEFPHTHMDRRPRKRARLGWDVLPQATKAQV--GMFCGQEIGNISSFASSGAPSDNSSSLCVKGVARNGSPPWREDDKDGHYIFELGDDLTPRYKIYSKMGEGTFGQVLECWDRERKEMVAVKIVRGVKKYREAAMIEIEMLQQLGKHDKGGNRCVQIRNWFDYRNHICIV 175

HSP 2 Score: 63.1586 bits (152), Expect = 3.279e-70
Identity = 32/49 (65.31%), Postives = 40/49 (81.63%), Query Frame = 2 
Query:  695 FMHELRLIHTDLKPXNILLVSAEYVKVPDYKFLSRSSKDGSYFKNLPKS 841
            FMH+LR+IHTDLKP NILLVS++YVK+P+YK  SR  +D  Y K +PKS
Sbjct:  213 FMHDLRMIHTDLKPENILLVSSDYVKIPEYKG-SRLQRDVCY-KRVPKS 259
[Close]
BLAST of EY754176 vs. ExPASy Swiss-Prot
Match: AFC3_ARATH (Serine/threonine-protein kinase AFC3 OS=Arabidopsis thaliana GN=AFC3 PE=2 SV=2)

HSP 1 Score: 175.637 bits (444), Expect = 6.971e-55
Identity = 90/161 (55.90%), Postives = 113/161 (70.19%), Query Frame = 3 
Query:  219 KNMDK-RPRKRPRLTWDXXXXXXXXXXXXALYCVQEFGNGGMPNYACSSMFYGAIPRTGSPPWRPDDKDGHYVFAIGENLTPRYRILSKMGEGTFGQVVECFDNEKKELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGTRCVQIRNWFDYRNHICIL 698
            ++MDK R RKRPR+TWD                ++  G+ G               R  SPP R DD+DGHYVF++ +NLTPRY+ILSKMGEGTFG+V+EC+D + KE VAIKI+RSI KYR+AAMIEIDVLQ+L ++D G TRCVQ++NWFDYRNHICI+
Sbjct:    7 ESMDKERVRKRPRMTWDEAPAEPEAKRAV----IKGHGSDG---------------RILSPPLRDDDRDGHYVFSLRDNLTPRYKILSKMGEGTFGRVLECWDRDTKEYVAIKIIRSIKKYRDAAMIEIDVLQKLVKSDKGRTRCVQMKNWFDYRNHICIV 148

HSP 2 Score: 60.4622 bits (145), Expect = 6.971e-55
Identity = 30/49 (61.22%), Postives = 40/49 (81.63%), Query Frame = 2 
Query:  695 FMHELRLIHTDLKPXNILLVSAEYVKVPDYKFLSRSSKDGSYFKNLPKS 841
            +MHEL+L+HTDLKP NILLVS+E VK+PD K   RS+ + ++F+ LPKS
Sbjct:  186 YMHELQLVHTDLKPENILLVSSENVKLPDNK---RSAANETHFRCLPKS 231
[Close]
BLAST of EY754176 vs. ExPASy Swiss-Prot
Match: LKH1_SCHPO (Dual specificity protein kinase lkh1 OS=Schizosaccharomyces pombe GN=lkh1 PE=1 SV=3)

HSP 1 Score: 99.3673 bits (246), Expect = 3.907e-25
Identity = 46/105 (43.81%), Postives = 66/105 (62.86%), Query Frame = 3 
Query:  387 TGSPPWRPDDKDGHYVFAIGENLTPRYRILSKMGEGTFGQVVECFDNEKKELVAIKIVRSINKYREAAMIEIDVLQRLARND-IGGTRCVQIRNWFDYRNHICIL 698
            T   P   DD DGHY          RY ++  +G GTFG+V++C+D       AIK+ R+I KYREA++IE+ VLQ +A +D     +C+Q+R++FDYR HICI+
Sbjct:  336 TAFDPSTFDDDDGHYKVVPNSKFANRYTVVRLLGHGTFGKVIQCYDQSTGRHCAIKVTRAIPKYREASLIELRVLQTIAHSDPTNENKCIQLRDYFDYRKHICIV 440

HSP 2 Score: 36.965 bits (84), Expect = 3.907e-25
Identity = 15/21 (71.43%), Postives = 18/21 (85.71%), Query Frame = 2 
Query:  695 FMHELRLIHTDLKPXNILLVS 757
            F+H L L+HTDLKP N+LLVS
Sbjct:  478 FLHSLGLVHTDLKPENVLLVS 498
[Close]
BLAST of EY754176 vs. ExPASy Swiss-Prot
Match: CLK3_MOUSE (Dual specificity protein kinase CLK3 OS=Mus musculus GN=Clk3 PE=1 SV=2)

HSP 1 Score: 98.2117 bits (243), Expect = 5.091e-25
Identity = 47/97 (48.45%), Postives = 68/97 (70.10%), Query Frame = 3 
Query:  411 DDKDGHYVFAIGENLTPRYRILSKMGEGTFGQVVECFDNEK-KELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGT-RCVQIRNWFDYRNHICI 695
            DDK+GH V  IG+ L  RY I+  +GEGTFG+VVEC D+ + K  VA+KI+R++ KYREAA +EI+VL+++   D      CV + +WF++  H+CI
Sbjct:  286 DDKEGHLVCRIGDWLQERYEIVGNLGEGTFGKVVECLDHARGKSQVALKIIRNVGKYREAARLEINVLKKIKEKDKENKFLCVLMSDWFNFHGHMCI 382

HSP 2 Score: 37.7354 bits (86), Expect = 5.091e-25
Identity = 16/26 (61.54%), Postives = 21/26 (80.77%), Query Frame = 2 
Query:  689 LYFMHELRLIHTDLKPXNILLVSAEY 766
            L F+HE +L HTDLKP NIL V++E+
Sbjct:  419 LRFLHENQLTHTDLKPENILFVNSEF 444
[Close]
BLAST of EY754176 vs. ExPASy Swiss-Prot
Match: CLK3_HUMAN (Dual specificity protein kinase CLK3 OS=Homo sapiens GN=CLK3 PE=1 SV=3)

HSP 1 Score: 98.2117 bits (243), Expect = 5.091e-25
Identity = 47/97 (48.45%), Postives = 68/97 (70.10%), Query Frame = 3 
Query:  411 DDKDGHYVFAIGENLTPRYRILSKMGEGTFGQVVECFDNEK-KELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGT-RCVQIRNWFDYRNHICI 695
            DDK+GH V  IG+ L  RY I+  +GEGTFG+VVEC D+ + K  VA+KI+R++ KYREAA +EI+VL+++   D      CV + +WF++  H+CI
Sbjct:  286 DDKEGHLVCRIGDWLQERYEIVGNLGEGTFGKVVECLDHARGKSQVALKIIRNVGKYREAARLEINVLKKIKEKDKENKFLCVLMSDWFNFHGHMCI 382

HSP 2 Score: 37.7354 bits (86), Expect = 5.091e-25
Identity = 16/26 (61.54%), Postives = 21/26 (80.77%), Query Frame = 2 
Query:  689 LYFMHELRLIHTDLKPXNILLVSAEY 766
            L F+HE +L HTDLKP NIL V++E+
Sbjct:  419 LRFLHENQLTHTDLKPENILFVNSEF 444
[Close]
BLAST of EY754176 vs. ExPASy Swiss-Prot
Match: CLK3_RAT (Dual specificity protein kinase CLK3 OS=Rattus norvegicus GN=Clk3 PE=2 SV=2)

HSP 1 Score: 98.2117 bits (243), Expect = 5.114e-25
Identity = 47/97 (48.45%), Postives = 68/97 (70.10%), Query Frame = 3 
Query:  411 DDKDGHYVFAIGENLTPRYRILSKMGEGTFGQVVECFDNEK-KELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGT-RCVQIRNWFDYRNHICI 695
            DDK+GH V  IG+ L  RY I+  +GEGTFG+VVEC D+ + K  VA+KI+R++ KYREAA +EI+VL+++   D      CV + +WF++  H+CI
Sbjct:  138 DDKEGHLVCRIGDWLQERYEIVGNLGEGTFGKVVECLDHARGKSQVALKIIRNVGKYREAARLEINVLKKIKEKDKENKFLCVLMSDWFNFHGHMCI 234

HSP 2 Score: 37.7354 bits (86), Expect = 5.114e-25
Identity = 16/26 (61.54%), Postives = 21/26 (80.77%), Query Frame = 2 
Query:  689 LYFMHELRLIHTDLKPXNILLVSAEY 766
            L F+HE +L HTDLKP NIL V++E+
Sbjct:  271 LRFLHENQLTHTDLKPENILFVNSEF 296
[Close]
BLAST of EY754176 vs. ExPASy Swiss-Prot
Match: CLK3_BOVIN (Dual specificity protein kinase CLK3 OS=Bos taurus GN=CLK3 PE=2 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 5.114e-25
Identity = 47/97 (48.45%), Postives = 68/97 (70.10%), Query Frame = 3 
Query:  411 DDKDGHYVFAIGENLTPRYRILSKMGEGTFGQVVECFDNEK-KELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGT-RCVQIRNWFDYRNHICI 695
            DDK+GH V  IG+ L  RY I+  +GEGTFG+VVEC D+ + K  VA+KI+R++ KYREAA +EI+VL+++   D      CV + +WF++  H+CI
Sbjct:  138 DDKEGHLVCRIGDWLQERYEIVGNLGEGTFGKVVECLDHARGKSQVALKIIRNVGKYREAARLEINVLKKIKEKDKENKFLCVLMSDWFNFHGHMCI 234

HSP 2 Score: 37.7354 bits (86), Expect = 5.114e-25
Identity = 16/26 (61.54%), Postives = 21/26 (80.77%), Query Frame = 2 
Query:  689 LYFMHELRLIHTDLKPXNILLVSAEY 766
            L F+HE +L HTDLKP NIL V++E+
Sbjct:  271 LRFLHENQLTHTDLKPENILFVNSEF 296
[Close]
BLAST of EY754176 vs. ExPASy Swiss-Prot
Match: DOA_DROME (Serine/threonine-protein kinase Doa OS=Drosophila melanogaster GN=Doa PE=1 SV=2)

HSP 1 Score: 96.6709 bits (239), Expect = 1.117e-24
Identity = 43/97 (44.33%), Postives = 70/97 (72.16%), Query Frame = 3 
Query:  411 DDKDGHYVFAIGENLTPRYRILSKMGEGTFGQVVECFDNEKKELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGTR-CVQIRNWFDYRNHICIL 698
            DD DGH ++  G+ L  RY+I++ +GEGTFG+VV+  D E+   +A+KI++++ KYREAA +EI+ L+++A+ D      CV++ +WFDY  H+CI+
Sbjct:  461 DDADGHLIYHTGDILHHRYKIMATLGEGTFGRVVKVKDMERDYCMALKIIKNVEKYREAAKLEINALEKIAQKDPHCDHLCVKMIDWFDYHGHMCIV 557

HSP 2 Score: 38.1206 bits (87), Expect = 1.117e-24
Identity = 16/31 (51.61%), Postives = 22/31 (70.97%), Query Frame = 2 
Query:  695 FMHELRLIHTDLKPXNILLVSAEYVKVPDYK 787
            F+H+ RL HTDLKP NIL V ++Y    ++K
Sbjct:  595 FLHDNRLTHTDLKPENILFVDSDYTSHYNHK 625
[Close]
BLAST of EY754176 vs. ExPASy Swiss-Prot
Match: CLK2_HUMAN (Dual specificity protein kinase CLK2 OS=Homo sapiens GN=CLK2 PE=1 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 1.466e-24
Identity = 44/97 (45.36%), Postives = 69/97 (71.13%), Query Frame = 3 
Query:  411 DDKDGHYVFAIGENLTPRYRILSKMGEGTFGQVVECFDNEK-KELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGTR-CVQIRNWFDYRNHICI 695
            DD +GH ++ +G+ L  RY I+S +GEGTFG+VV+C D+ +    VA+KI++++ KY+EAA +EI+VL+++   D      CVQ+ +WFDY  H+CI
Sbjct:  145 DDAEGHLIYHVGDWLQERYEIVSTLGEGTFGRVVQCVDHRRGGARVALKIIKNVEKYKEAARLEINVLEKINEKDPDNKNLCVQMFDWFDYHGHMCI 241

HSP 2 Score: 36.5798 bits (83), Expect = 1.466e-24
Identity = 14/24 (58.33%), Postives = 20/24 (83.33%), Query Frame = 2 
Query:  695 FMHELRLIHTDLKPXNILLVSAEY 766
            F+H+ +L HTDLKP NIL V+++Y
Sbjct:  280 FLHDNKLTHTDLKPENILFVNSDY 303
The following BLAST results are available for this feature:
BLAST of EY754176 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 53
Match NameE-valueIdentityDescription
AFC1_ARATH1.036e-8968.18Serine/threonine-protein kinase AFC1 OS=Arabidopsi... [more]
AFC2_ARATH3.279e-7059.32Serine/threonine-protein kinase AFC2 OS=Arabidopsi... [more]
AFC3_ARATH6.971e-5555.90Serine/threonine-protein kinase AFC3 OS=Arabidopsi... [more]
LKH1_SCHPO3.907e-2543.81Dual specificity protein kinase lkh1 OS=Schizosacc... [more]
CLK3_MOUSE5.091e-2548.45Dual specificity protein kinase CLK3 OS=Mus muscul... [more]
CLK3_HUMAN5.091e-2548.45Dual specificity protein kinase CLK3 OS=Homo sapie... [more]
CLK3_RAT5.114e-2548.45Dual specificity protein kinase CLK3 OS=Rattus nor... [more]
CLK3_BOVIN5.114e-2548.45Dual specificity protein kinase CLK3 OS=Bos taurus... [more]
DOA_DROME1.117e-2444.33Serine/threonine-protein kinase Doa OS=Drosophila ... [more]
CLK2_HUMAN1.466e-2445.36Dual specificity protein kinase CLK2 OS=Homo sapie... [more]

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Properties
Property NameValue
Genbank descriptionCS12-C1-001-011-F03-CT.F Sweet orange leaf, field plant A Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY754176 ID=EY754176; Name=EY754176; organism=Citrus sinensis; type=EST; length=921bp
CCGGNATTCCACGCTCGACCCACGCGTCCGAATCCCCTTTTAGGGTTTCC
AACACTNGAAAATCATCTCTTCAAAATTTCCTTAATTTTTTACTCTCAAT
TTTTTTTAAAGCATTATGATTCAAATTGAGCTTAATCCATGGCTCTGCTA
GGTTTTTTTAATTGAAAATTTAAACAATAAAAGAAATGGAAACGCAGAGA
ATTATAGAATTTCCGCATAAAAACATGGACAAGCGTCCCAGAAAACGACC
CCGTTTAACGTGGGACGTTCCGCCTCCTCTTCCTCCTCCCAAGGTTCTTC
CGGCTCTGTATTGTGTTCAGGAGTTTGGGAATGGAGGAATGCCCAATTAT
GCCTGTTCATCCATGTTTTATGGTGCGATTCCTCGCACCGGATCACCTCC
TTGGAGACCTGATGATAAAGACGGACATTACGTTTTTGCCATCGGAGAAA
ATTTAACTCCTCGCTACAGGATTCTCAGCAAAATGGGTGAAGGGACATTT
GGGCAAGTCGTGGAATGTTTTGACAATGAGAAGAAGGAACTTGTGGCAAT
CAAAATTGTTCGCTCTATAAACAAGTATCGTGAAGCAGCCATGATCGAAA
TTGATGTTCTACAGAGGCTTGCCAGGAATGACATTGGTGGCACCCGTTGT
GTGCAAATACGGAATTGGTTTGACTATCGTAATCATATTTGTATTTTATG
CATGAATTACGTCTTATACACACTGACTTGAAGCCANGAAATATTCTTCT
TGTTTCCGCTGAGTATGTTAAAGTCCCTGATTATAAGTTTCTATCACGAT
CGTCAAAAGATGGTTCCTATTTCAAGAATCTCCCCAAGTCAGTGCATTTA
GCTAATTGATTTGGGAACACACATTTGAACATCAGATCCAGCTTTTGTGG
TTAACACCACTTATCTGCCCC
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