EY727705

Overview
NameEY727705
Unique NameEY727705
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length1059
Libraries
Library NameType
Sweet orange fruit, development stadium (4 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY727705 vs. ExPASy Swiss-Prot
Match: HMCS_ARATH (Hydroxymethylglutaryl-CoA synthase OS=Arabidopsis thaliana GN=HMGS PE=2 SV=2)

HSP 1 Score: 175.252 bits (443), Expect = 9.718e-54
Identity = 85/95 (89.47%), Postives = 91/95 (95.79%), Query Frame = 2 
Query:  263 MAKNVGILAMDIYFPPTCVQQEALEAHDGASKGKYTIGLGQECMAFCSEVEDVISMSLTAVTNLLEKYKIDPKQIGRLEVGSETVINKSKSIKTF 547
            MAKNVGILAMDIYFPPTCVQQEALEAHDGASKGKYTIGLGQ+C+AFC+E+EDVISMS  AVT+L EKYKIDP QIGRLEVGSETVI+KSKSIKTF
Sbjct:    1 MAKNVGILAMDIYFPPTCVQQEALEAHDGASKGKYTIGLGQDCLAFCTELEDVISMSFNAVTSLFEKYKIDPNQIGRLEVGSETVIDKSKSIKTF 95

HSP 2 Score: 57.3806 bits (137), Expect = 9.718e-54
Identity = 32/69 (46.38%), Postives = 44/69 (63.77%), Query Frame = 3 
Query:  525 RANPLRLFLMQIFENFGNTDIEGVDSTHACLRRTGGLFYWGNGVKSISGNGRLGPRWGSRTWGVYAKGP 731
            ++  ++ FLMQ+FE  GNTD+EGVDST+AC   T  L    N V+S S +GR G    + +  VYA+GP
Sbjct:   88 KSKSIKTFLMQLFEKCGNTDVEGVDSTNACYGGTAALLNCVNWVESNSWDGRYGLVICTDS-AVYAEGP 155
[Close]
BLAST of EY727705 vs. ExPASy Swiss-Prot
Match: HMCS1_CHICK (Hydroxymethylglutaryl-CoA synthase, cytoplasmic OS=Gallus gallus GN=HMGCS1 PE=1 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 5.560e-31
Identity = 52/92 (56.52%), Postives = 67/92 (72.83%), Query Frame = 2 
Query:  269 KNVGILAMDIYFPPTCVQQEALEAHDGASKGKYTIGLGQECMAFCSEVEDVISMSLTAVTNLLEKYKIDPKQIGRLEVGSETVINKSKSIKT 544
            K+VGI+A++IYFP   V Q  LE +DG   GKYTIGLGQ  M FCS+ ED+ S+ LT V  L+E+  +    IGRLEVG+ET+I+KSKS+KT
Sbjct:   15 KDVGIVALEIYFPSQYVDQTELEKYDGVDAGKYTIGLGQSKMGFCSDREDINSLCLTVVQKLMERNSLSYDCIGRLEVGTETIIDKSKSVKT 106

HSP 2 Score: 50.447 bits (119), Expect = 5.560e-31
Identity = 23/52 (44.23%), Postives = 35/52 (67.31%), Query Frame = 3 
Query:  525 RANPLRLFLMQIFENFGNTDIEGVDSTHACLRRTGGLFYWGNGVKSISGNGR 680
            ++  ++  LMQ+FE  GNTD+EG+D+T+AC   T  LF   N ++S S +GR
Sbjct:  100 KSKSVKTVLMQLFEESGNTDVEGIDTTNACYGGTAALFNAINWIESSSWDGR 151
[Close]
BLAST of EY727705 vs. ExPASy Swiss-Prot
Match: HMCS1_PONAB (Hydroxymethylglutaryl-CoA synthase, cytoplasmic OS=Pongo abelii GN=HMGCS1 PE=2 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 9.428e-31
Identity = 51/92 (55.43%), Postives = 69/92 (75.00%), Query Frame = 2 
Query:  269 KNVGILAMDIYFPPTCVQQEALEAHDGASKGKYTIGLGQECMAFCSEVEDVISMSLTAVTNLLEKYKIDPKQIGRLEVGSETVINKSKSIKT 544
            K+VGI+A++IYFP   V Q  LE +DG + GKYTIGLGQ  M FC++ ED+ S+ +T V NL+E+  +    IGRLEVG+ET+I+KSKS+KT
Sbjct:   15 KDVGIVALEIYFPSQYVDQAELEKYDGVAAGKYTIGLGQAKMGFCTDREDINSLCMTVVQNLMERNNLSYDCIGRLEVGTETIIDKSKSVKT 106

HSP 2 Score: 49.2914 bits (116), Expect = 9.428e-31
Identity = 23/52 (44.23%), Postives = 35/52 (67.31%), Query Frame = 3 
Query:  525 RANPLRLFLMQIFENFGNTDIEGVDSTHACLRRTGGLFYWGNGVKSISGNGR 680
            ++  ++  LMQ+FE  GNTDIEG+D+T+AC   T  +F   N ++S S +GR
Sbjct:  100 KSKSVKTNLMQLFEESGNTDIEGIDTTNACYGGTAAVFNAVNWIESSSWDGR 151
[Close]
BLAST of EY727705 vs. ExPASy Swiss-Prot
Match: HMCS1_HUMAN (Hydroxymethylglutaryl-CoA synthase, cytoplasmic OS=Homo sapiens GN=HMGCS1 PE=1 SV=2)

HSP 1 Score: 105.916 bits (263), Expect = 1.228e-30
Identity = 51/92 (55.43%), Postives = 68/92 (73.91%), Query Frame = 2 
Query:  269 KNVGILAMDIYFPPTCVQQEALEAHDGASKGKYTIGLGQECMAFCSEVEDVISMSLTAVTNLLEKYKIDPKQIGRLEVGSETVINKSKSIKT 544
            K+VGI+A++IYFP   V Q  LE +DG   GKYTIGLGQ  M FC++ ED+ S+ +T V NL+E+  +    IGRLEVG+ET+I+KSKS+KT
Sbjct:   15 KDVGIVALEIYFPSQYVDQAELEKYDGVDAGKYTIGLGQAKMGFCTDREDINSLCMTVVQNLMERNNLSYDCIGRLEVGTETIIDKSKSVKT 106

HSP 2 Score: 49.2914 bits (116), Expect = 1.228e-30
Identity = 23/52 (44.23%), Postives = 35/52 (67.31%), Query Frame = 3 
Query:  525 RANPLRLFLMQIFENFGNTDIEGVDSTHACLRRTGGLFYWGNGVKSISGNGR 680
            ++  ++  LMQ+FE  GNTDIEG+D+T+AC   T  +F   N ++S S +GR
Sbjct:  100 KSKSVKTNLMQLFEESGNTDIEGIDTTNACYGGTAAVFNAVNWIESSSWDGR 151
[Close]
BLAST of EY727705 vs. ExPASy Swiss-Prot
Match: HMCSA_DICDI (Hydroxymethylglutaryl-CoA synthase A OS=Dictyostelium discoideum GN=hgsA PE=1 SV=2)

HSP 1 Score: 121.324 bits (303), Expect = 1.600e-30
Identity = 56/92 (60.87%), Postives = 73/92 (79.35%), Query Frame = 2 
Query:  269 KNVGILAMDIYFPPTCVQQEALEAHDGASKGKYTIGLGQECMAFCSEVEDVISMSLTAVTNLLEKYKIDPKQIGRLEVGSETVINKSKSIKT 544
            +N+GI  +++YFP T V QE LE  DG S+GKYT+GLGQ  MAFC + ED+ S+SL AV NL++K+ +DP  IGRLEVG+ETVI+KSKS+KT
Sbjct:    5 ENIGIHGIEVYFPSTYVAQEDLEKFDGVSQGKYTLGLGQTNMAFCGDREDIYSLSLNAVNNLMDKFNVDPNSIGRLEVGTETVIDKSKSVKT 96

HSP 2 Score: 33.4982 bits (75), Expect = 1.600e-30
Identity = 21/69 (30.43%), Postives = 34/69 (49.28%), Query Frame = 3 
Query:  525 RANPLRLFLMQIFENFGNTDIEGVDSTHACLRRTGGLFYWGNGVKSISGNGRLGPRWGSRTWGVYAKGP 731
            ++  ++  LM +F   GNT I+G+D+ +AC   T  L      ++S   +GR      +    VY KGP
Sbjct:   90 KSKSVKTVLMDLFAKHGNTSIDGIDTINACYGGTSALHNALQWMESSYWDGR-NAIVVAGDIAVYEKGP 157
[Close]
BLAST of EY727705 vs. ExPASy Swiss-Prot
Match: HMCS1_CRIGR (Hydroxymethylglutaryl-CoA synthase, cytoplasmic OS=Cricetulus griseus GN=HMGCS1 PE=3 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 2.710e-30
Identity = 51/92 (55.43%), Postives = 68/92 (73.91%), Query Frame = 2 
Query:  269 KNVGILAMDIYFPPTCVQQEALEAHDGASKGKYTIGLGQECMAFCSEVEDVISMSLTAVTNLLEKYKIDPKQIGRLEVGSETVINKSKSIKT 544
            K+VGI+A++IYFP   V Q  LE +DG   GKYTIGLGQ  M FC++ ED+ S+ LT V NL+E+  +    IGRLEVG+ET+I+KSKS+K+
Sbjct:   15 KDVGIVALEIYFPSQYVDQAELEKYDGVDAGKYTIGLGQARMGFCTDREDINSLCLTVVQNLMERNSLSYDCIGRLEVGTETIIDKSKSVKS 106

HSP 2 Score: 48.9062 bits (115), Expect = 2.710e-30
Identity = 23/44 (52.27%), Postives = 31/44 (70.45%), Query Frame = 3 
Query:  549 LMQIFENFGNTDIEGVDSTHACLRRTGGLFYWGNGVKSISGNGR 680
            LMQ+FE  GNTDIEG+D+T+AC   T  +F   N ++S S +GR
Sbjct:  108 LMQLFEESGNTDIEGIDTTNACYGGTAAVFNAVNWIESSSWDGR 151
[Close]
BLAST of EY727705 vs. ExPASy Swiss-Prot
Match: HMCS1_MOUSE (Hydroxymethylglutaryl-CoA synthase, cytoplasmic OS=Mus musculus GN=Hmgcs1 PE=1 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 3.529e-30
Identity = 50/92 (54.35%), Postives = 68/92 (73.91%), Query Frame = 2 
Query:  269 KNVGILAMDIYFPPTCVQQEALEAHDGASKGKYTIGLGQECMAFCSEVEDVISMSLTAVTNLLEKYKIDPKQIGRLEVGSETVINKSKSIKT 544
            K+VGI+A++IYFP   V Q  LE +DG   GKYTIGLGQ  M FC++ ED+ S+ LT V  L+E++ +    IGRLEVG+ET+I+KSKS+K+
Sbjct:   15 KDVGIVALEIYFPSQYVDQAELEKYDGVDAGKYTIGLGQARMGFCTDREDINSLCLTVVQKLMERHSLSYDCIGRLEVGTETIIDKSKSVKS 106

HSP 2 Score: 49.2914 bits (116), Expect = 3.529e-30
Identity = 24/44 (54.55%), Postives = 31/44 (70.45%), Query Frame = 3 
Query:  549 LMQIFENFGNTDIEGVDSTHACLRRTGGLFYWGNGVKSISGNGR 680
            LMQ+FE  GNTDIEG+D+T+AC   T  +F   N V+S S +GR
Sbjct:  108 LMQLFEESGNTDIEGIDTTNACYGGTAAVFNAVNWVESSSWDGR 151
[Close]
BLAST of EY727705 vs. ExPASy Swiss-Prot
Match: HMCS1_RAT (Hydroxymethylglutaryl-CoA synthase, cytoplasmic OS=Rattus norvegicus GN=Hmgcs1 PE=1 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 1.321e-29
Identity = 50/92 (54.35%), Postives = 67/92 (72.83%), Query Frame = 2 
Query:  269 KNVGILAMDIYFPPTCVQQEALEAHDGASKGKYTIGLGQECMAFCSEVEDVISMSLTAVTNLLEKYKIDPKQIGRLEVGSETVINKSKSIKT 544
            K+VGI+A++IYFP   V Q  LE +DG   GKYTIGLGQ  M FC++ ED+ S+ LT V  L+E+  +    IGRLEVG+ET+I+KSKS+K+
Sbjct:   15 KDVGIVALEIYFPSQYVDQAELEKYDGVDAGKYTIGLGQARMGFCTDREDINSLCLTVVQKLMERNSLSYDCIGRLEVGTETIIDKSKSVKS 106

HSP 2 Score: 48.9062 bits (115), Expect = 1.321e-29
Identity = 23/44 (52.27%), Postives = 31/44 (70.45%), Query Frame = 3 
Query:  549 LMQIFENFGNTDIEGVDSTHACLRRTGGLFYWGNGVKSISGNGR 680
            LMQ+FE  GNTDIEG+D+T+AC   T  +F   N ++S S +GR
Sbjct:  108 LMQLFEESGNTDIEGIDTTNACYGGTAAVFNAVNWIESSSWDGR 151
[Close]
BLAST of EY727705 vs. ExPASy Swiss-Prot
Match: HMCS2_HUMAN (Hydroxymethylglutaryl-CoA synthase, mitochondrial OS=Homo sapiens GN=HMGCS2 PE=1 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 6.435e-29
Identity = 47/92 (51.09%), Postives = 66/92 (71.74%), Query Frame = 2 
Query:  269 KNVGILAMDIYFPPTCVQQEALEAHDGASKGKYTIGLGQECMAFCSEVEDVISMSLTAVTNLLEKYKIDPKQIGRLEVGSETVINKSKSIKT 544
            K+VGILA+++YFP   V Q  LE ++    GKYT+GLGQ  M FCS  ED+ S+ LT V  L+E+ ++    +GRLEVG+ET+I+KSK++KT
Sbjct:   52 KDVGILALEVYFPAQYVDQTDLEKYNNVEAGKYTVGLGQTRMGFCSVQEDINSLCLTVVQRLMERIQLPWDSVGRLEVGTETIIDKSKAVKT 143

HSP 2 Score: 49.6766 bits (117), Expect = 6.435e-29
Identity = 22/52 (42.31%), Postives = 36/52 (69.23%), Query Frame = 3 
Query:  525 RANPLRLFLMQIFENFGNTDIEGVDSTHACLRRTGGLFYWGNGVKSISGNGR 680
            ++  ++  LM++F++ GNTDIEG+D+T+AC   T  LF   N ++S S +GR
Sbjct:  137 KSKAVKTVLMELFQDSGNTDIEGIDTTNACYGGTASLFNAANWMESSSWDGR 188
[Close]
BLAST of EY727705 vs. ExPASy Swiss-Prot
Match: HMCS2_BOVIN (Hydroxymethylglutaryl-CoA synthase, mitochondrial OS=Bos taurus GN=HMGCS2 PE=2 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 1.849e-28
Identity = 48/92 (52.17%), Postives = 65/92 (70.65%), Query Frame = 2 
Query:  269 KNVGILAMDIYFPPTCVQQEALEAHDGASKGKYTIGLGQECMAFCSEVEDVISMSLTAVTNLLEKYKIDPKQIGRLEVGSETVINKSKSIKT 544
            K+VGILAM++YFP   V Q  LE  +    G+YT+GLGQ  M FCS  EDV S+ LT V  L+E+ ++    +GRLEVG+ET+I+KSK++KT
Sbjct:   52 KDVGILAMEVYFPAQYVDQTELEKFNKVEAGRYTVGLGQTQMGFCSVQEDVNSLCLTVVQQLMERTQLPWDSVGRLEVGTETIIDKSKAVKT 143

HSP 2 Score: 49.6766 bits (117), Expect = 1.849e-28
Identity = 22/52 (42.31%), Postives = 36/52 (69.23%), Query Frame = 3 
Query:  525 RANPLRLFLMQIFENFGNTDIEGVDSTHACLRRTGGLFYWGNGVKSISGNGR 680
            ++  ++  LM++F++ GNTDIEG+D+T+AC   T  LF   N ++S S +GR
Sbjct:  137 KSKAVKTVLMELFQDSGNTDIEGIDTTNACYGGTASLFNAANWMESSSWDGR 188
The following BLAST results are available for this feature:
BLAST of EY727705 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 19
Match NameE-valueIdentityDescription
HMCS_ARATH9.718e-5489.47Hydroxymethylglutaryl-CoA synthase OS=Arabidopsis ... [more]
HMCS1_CHICK5.560e-3156.52Hydroxymethylglutaryl-CoA synthase, cytoplasmic OS... [more]
HMCS1_PONAB9.428e-3155.43Hydroxymethylglutaryl-CoA synthase, cytoplasmic OS... [more]
HMCS1_HUMAN1.228e-3055.43Hydroxymethylglutaryl-CoA synthase, cytoplasmic OS... [more]
HMCSA_DICDI1.600e-3060.87Hydroxymethylglutaryl-CoA synthase A OS=Dictyostel... [more]
HMCS1_CRIGR2.710e-3055.43Hydroxymethylglutaryl-CoA synthase, cytoplasmic OS... [more]
HMCS1_MOUSE3.529e-3054.35Hydroxymethylglutaryl-CoA synthase, cytoplasmic OS... [more]
HMCS1_RAT1.321e-2954.35Hydroxymethylglutaryl-CoA synthase, cytoplasmic OS... [more]
HMCS2_HUMAN6.435e-2951.09Hydroxymethylglutaryl-CoA synthase, mitochondrial ... [more]
HMCS2_BOVIN1.849e-2852.17Hydroxymethylglutaryl-CoA synthase, mitochondrial ... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-703-104-F03-CT.F Sweet orange fruit, development stadium (4 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY727705 ID=EY727705; Name=EY727705; organism=Citrus sinensis; type=EST; length=1059bp
GTGATTGTTCGGGACGGCGGTACGGTCGGGATTCCCGGGTCGACCACGCG
TCCGCTCCGAATATCGAGGCCTGTCTTCTCTTTCTGTCTCTGAACGGCTA
GCTGGGCTACGTTTTACAAACTCAAGCCAACAGCAGAGCAAGAAAGAAAG
CAAAGCAACGCAAAACACATTTTCGTTGTTGTGCTGTTTTCTTGATACAT
ATATATATTTACATATATTTTTCATCTGGGCATTTTCTGTTTTTGAAAAC
CAGATAAAAGCAATGGCAAAGAATGTTGGAATCCTCGCTATGGACATCTA
CTTCCCTCCTACTTGCGTCCAGCAGGAAGCTTTGGAGGCTCATGATGGTG
CAAGCAAAGGAAAATATACAATCGGGCTTGGACAGGAGTGCATGGCATTT
TGTTCAGAGGTGGAAGATGTCATCTCAATGAGCTTGACAGCTGTTACTAA
TCTCCTAGAGAAGTATAAGATTGATCCAAAACAAATTGGTCGTCTCGAAG
TAGGAAGCGAAACTGTGATAAACAAGAGCAAATCCATTAAGACTTTTTTT
GATGCAAATTTTTGAGAATTTTGGAAATACTGACATTGAAGGGGTTGACT
CCACCCATGCCTGCTTACGGAGAACTGGCGGATTATTTTACTGGGGGAAT
GGGGTGAAAAGTATTTCAGGGAACGGGCGCCTAGGGCCTCGTTGGGGGTC
CCGAACGTGGGGGGTTTATGCGAAAGGGCCCCTCCAAAACCCCCTGGAAG
AACCCGCTGGCGGTTTGTCAGGTTAATAAGGGGCTGGAAAGCCCCTGGTT
TGGTTTTTGAAAAGCAAATTTAGGGGGGGAATCCTAAAGTTCCCCAGGGG
CTAGTAATTTTTCCACCAACCCCAAAACCTTGGGGATGGGGAAAAATTCC
CGGGGTGGGTGGAAGGGGGGGAAAATTTTTTTTCCAAAAACAGGGGTCTC
CCCCCCCGGGGGGGCCTTTTTGTTAAAACTTGTTGTTTAAAAAAAAGTGT
TTTTTTATTGGTGGGGCGGGAAACAGAACCCAACAAAAAAAAAACACAGG
GGGGGGGAG
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