GE213218

Overview
NameGE213218
Unique NameGE213218
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length589
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig11493 contig Csv1_Contig11493:598..1186. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of GE213218 vs. ExPASy Swiss-Prot
Match: GLNA1_VITVI (Glutamine synthetase cytosolic isozyme 1 OS=Vitis vinifera GN=GS1-1 PE=2 SV=1)

HSP 1 Score: 271.552 bits (693), Expect = 2.106e-72
Identity = 131/139 (94.24%), Postives = 133/139 (95.68%), Query Frame = -1 
Query:  155 RYILERITEIAGVVLSFDPKPIKGDWNGAGAHANYSTKSMRNDGGIDVIKKAIEKLGKRHGEHIAAYGEGNERRLTGRHETADINTFSWGVANRGASIRVGRDTEKEGKGYFEDRRPASNMDPYVVTSMIAETTILWKP 571
            RYILERITEIAGVVLSFDPKPI+GDWNGAGAH NYSTKSMRNDGG +VIKKAIEKLG RH EHIAAYGEGNERRLTGRHETADINTF WGVANRGASIRVGRDTEK GKGYFEDRRPASNMDPYVVTSMIAETTILWKP
Sbjct:  218 RYILERITEIAGVVLSFDPKPIQGDWNGAGAHTNYSTKSMRNDGGFEVIKKAIEKLGLRHKEHIAAYGEGNERRLTGRHETADINTFLWGVANRGASIRVGRDTEKAGKGYFEDRRPASNMDPYVVTSMIAETTILWKP 356
[Close]
BLAST of GE213218 vs. ExPASy Swiss-Prot
Match: GLNA_VIGAC (Glutamine synthetase nodule isozyme OS=Vigna aconitifolia PE=2 SV=1)

HSP 1 Score: 271.166 bits (692), Expect = 2.751e-72
Identity = 130/139 (93.53%), Postives = 133/139 (95.68%), Query Frame = -1 
Query:  155 RYILERITEIAGVVLSFDPKPIKGDWNGAGAHANYSTKSMRNDGGIDVIKKAIEKLGKRHGEHIAAYGEGNERRLTGRHETADINTFSWGVANRGASIRVGRDTEKEGKGYFEDRRPASNMDPYVVTSMIAETTILWKP 571
            RYILERITEIAGVVLSFDPKPIKGDWNGAGAH NYSTK+MRNDGG +VIK AIEKLGKRH EHIAAYGEGNERRLTGRHETADINTF WGVANRGASIRVGRDTEK GKGYFEDRRPASNMDPYVVTSMIA+TTILWKP
Sbjct:  218 RYILERITEIAGVVLSFDPKPIKGDWNGAGAHTNYSTKTMRNDGGYEVIKSAIEKLGKRHKEHIAAYGEGNERRLTGRHETADINTFLWGVANRGASIRVGRDTEKAGKGYFEDRRPASNMDPYVVTSMIADTTILWKP 356
[Close]
BLAST of GE213218 vs. ExPASy Swiss-Prot
Match: GLNA2_VITVI (Glutamine synthetase cytosolic isozyme 2 OS=Vitis vinifera GN=GS1-2 PE=2 SV=1)

HSP 1 Score: 270.396 bits (690), Expect = 4.692e-72
Identity = 129/139 (92.81%), Postives = 134/139 (96.40%), Query Frame = -1 
Query:  155 RYILERITEIAGVVLSFDPKPIKGDWNGAGAHANYSTKSMRNDGGIDVIKKAIEKLGKRHGEHIAAYGEGNERRLTGRHETADINTFSWGVANRGASIRVGRDTEKEGKGYFEDRRPASNMDPYVVTSMIAETTILWKP 571
            RYILERITEIAGVV+SFDPKPI+GDWNGAGAH NYSTKSMRNDGG ++IKKAIEKLG RH EHIAAYGEGNERRLTGRHETADINTF WGVANRGASIRVGRDTEKEGKGYFEDRRPASNMDPYVVTSMIAE+TILWKP
Sbjct:  218 RYILERITEIAGVVVSFDPKPIQGDWNGAGAHTNYSTKSMRNDGGYEIIKKAIEKLGLRHKEHIAAYGEGNERRLTGRHETADINTFLWGVANRGASIRVGRDTEKEGKGYFEDRRPASNMDPYVVTSMIAESTILWKP 356
[Close]
BLAST of GE213218 vs. ExPASy Swiss-Prot
Match: GLNA1_LOTJA (Glutamine synthetase cytosolic isozyme OS=Lotus japonicus GN=GLN1 PE=2 SV=2)

HSP 1 Score: 269.24 bits (687), Expect = 1.045e-71
Identity = 129/139 (92.81%), Postives = 133/139 (95.68%), Query Frame = -1 
Query:  155 RYILERITEIAGVVLSFDPKPIKGDWNGAGAHANYSTKSMRNDGGIDVIKKAIEKLGKRHGEHIAAYGEGNERRLTGRHETADINTFSWGVANRGASIRVGRDTEKEGKGYFEDRRPASNMDPYVVTSMIAETTILWKP 571
            RYILERITEIAGVVLSFDPKPIKGDWNGAGAH NYSTK+MR DGG +VIKKAI+KLG RH EHIAAYGEGNERRLTGRHETADINTF WGVANRGASIRVGRDTEKEGKGYFEDRRPASNMDPYVVTSMIA+TTILWKP
Sbjct:  218 RYILERITEIAGVVLSFDPKPIKGDWNGAGAHTNYSTKTMREDGGYEVIKKAIDKLGLRHKEHIAAYGEGNERRLTGRHETADINTFLWGVANRGASIRVGRDTEKEGKGYFEDRRPASNMDPYVVTSMIADTTILWKP 356
[Close]
BLAST of GE213218 vs. ExPASy Swiss-Prot
Match: GLNA1_ALNGL (Glutamine synthetase OS=Alnus glutinosa GN=GLN1 PE=2 SV=1)

HSP 1 Score: 268.855 bits (686), Expect = 1.365e-71
Identity = 128/139 (92.09%), Postives = 133/139 (95.68%), Query Frame = -1 
Query:  155 RYILERITEIAGVVLSFDPKPIKGDWNGAGAHANYSTKSMRNDGGIDVIKKAIEKLGKRHGEHIAAYGEGNERRLTGRHETADINTFSWGVANRGASIRVGRDTEKEGKGYFEDRRPASNMDPYVVTSMIAETTILWKP 571
            RYILERITEIAGVVLS DPKPI+GDWNGAGAH NYSTKSMRN+GG ++IKKAIEKLG RH EHIAAYGEGNERRLTGRHETADINTF WGVANRGASIRVGRDTEKEGKGYFEDRRPASNMDPYVVTSMIAETT+LWKP
Sbjct:  218 RYILERITEIAGVVLSLDPKPIQGDWNGAGAHTNYSTKSMRNNGGYEIIKKAIEKLGLRHKEHIAAYGEGNERRLTGRHETADINTFKWGVANRGASIRVGRDTEKEGKGYFEDRRPASNMDPYVVTSMIAETTLLWKP 356
[Close]
BLAST of GE213218 vs. ExPASy Swiss-Prot
Match: GLNA2_SOYBN (Glutamine synthetase cytosolic isozyme 2 OS=Glycine max PE=2 SV=1)

HSP 1 Score: 267.314 bits (682), Expect = 3.972e-71
Identity = 129/139 (92.81%), Postives = 132/139 (94.96%), Query Frame = -1 
Query:  155 RYILERITEIAGVVLSFDPKPIKGDWNGAGAHANYSTKSMRNDGGIDVIKKAIEKLGKRHGEHIAAYGEGNERRLTGRHETADINTFSWGVANRGASIRVGRDTEKEGKGYFEDRRPASNMDPYVVTSMIAETTILWKP 571
            RYILERITEIAGVVLSFDPKPI+GDWNGAGAH NYSTKSMRNDGG +VIKKAI KL KRH EHIAAYGEGNERRLTGRHETAD+NTF WGVANRGASIRVGRDTEK GKGYFEDRRPASNMDPYVVTSMIAETTILWKP
Sbjct:  218 RYILERITEIAGVVLSFDPKPIQGDWNGAGAHTNYSTKSMRNDGGYEVIKKAIAKLEKRHKEHIAAYGEGNERRLTGRHETADMNTFVWGVANRGASIRVGRDTEKAGKGYFEDRRPASNMDPYVVTSMIAETTILWKP 356
[Close]
BLAST of GE213218 vs. ExPASy Swiss-Prot
Match: GLNA1_PHAVU (Glutamine synthetase PR-1 OS=Phaseolus vulgaris PE=2 SV=1)

HSP 1 Score: 267.314 bits (682), Expect = 3.972e-71
Identity = 127/139 (91.37%), Postives = 132/139 (94.96%), Query Frame = -1 
Query:  155 RYILERITEIAGVVLSFDPKPIKGDWNGAGAHANYSTKSMRNDGGIDVIKKAIEKLGKRHGEHIAAYGEGNERRLTGRHETADINTFSWGVANRGASIRVGRDTEKEGKGYFEDRRPASNMDPYVVTSMIAETTILWKP 571
            RYILERITE+AGVVLSFDPKPIKGDWNGAGAH NYSTK+MRNDGG + IK AI+KLGKRH EHIAAYGEGNERRLTGRHETADINTF WGVANRGASIRVGRDTEK GKGYFEDRRPASNMDPYVVTSMIA+TTILWKP
Sbjct:  218 RYILERITEVAGVVLSFDPKPIKGDWNGAGAHTNYSTKTMRNDGGYEEIKSAIQKLGKRHKEHIAAYGEGNERRLTGRHETADINTFLWGVANRGASIRVGRDTEKAGKGYFEDRRPASNMDPYVVTSMIADTTILWKP 356
[Close]
BLAST of GE213218 vs. ExPASy Swiss-Prot
Match: GLNA1_MEDSA (Glutamine synthetase cytosolic isozyme OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 266.544 bits (680), Expect = 6.776e-71
Identity = 127/139 (91.37%), Postives = 132/139 (94.96%), Query Frame = -1 
Query:  155 RYILERITEIAGVVLSFDPKPIKGDWNGAGAHANYSTKSMRNDGGIDVIKKAIEKLGKRHGEHIAAYGEGNERRLTGRHETADINTFSWGVANRGASIRVGRDTEKEGKGYFEDRRPASNMDPYVVTSMIAETTILWKP 571
            RYILERITE+AGVVLSFDPKPIKGDWNGAGAH NYSTKSMR DGG +VI KAIEKLGK+H EHIAAYGEGNERRLTGRHETADINTF WGVANRGASIRVGRDTEK GKGYFEDRRP+SNMDPYVVTSMIA+TTILWKP
Sbjct:  218 RYILERITEVAGVVLSFDPKPIKGDWNGAGAHTNYSTKSMREDGGYEVILKAIEKLGKKHKEHIAAYGEGNERRLTGRHETADINTFLWGVANRGASIRVGRDTEKAGKGYFEDRRPSSNMDPYVVTSMIADTTILWKP 356
[Close]
BLAST of GE213218 vs. ExPASy Swiss-Prot
Match: GLNA3_PHAVU (Glutamine synthetase N-1 OS=Phaseolus vulgaris GN=Gln-gamma PE=2 SV=2)

HSP 1 Score: 265.388 bits (677), Expect = 1.509e-70
Identity = 127/139 (91.37%), Postives = 132/139 (94.96%), Query Frame = -1 
Query:  155 RYILERITEIAGVVLSFDPKPIKGDWNGAGAHANYSTKSMRNDGGIDVIKKAIEKLGKRHGEHIAAYGEGNERRLTGRHETADINTFSWGVANRGASIRVGRDTEKEGKGYFEDRRPASNMDPYVVTSMIAETTILWKP 571
            RYILERITEIAGVVLSFDPKPI+GDWNGAGAH NYSTKSMRNDGG +VIKKAI KL KRH EHIAAYGEGNERRLTG+HETAD+NTF WGVANRGASIRVGRDTEK GKGYFEDRRPASNMDPYVVTSMIAETT+LWKP
Sbjct:  218 RYILERITEIAGVVLSFDPKPIQGDWNGAGAHTNYSTKSMRNDGGYEVIKKAITKLEKRHKEHIAAYGEGNERRLTGKHETADMNTFIWGVANRGASIRVGRDTEKAGKGYFEDRRPASNMDPYVVTSMIAETTLLWKP 356
[Close]
BLAST of GE213218 vs. ExPASy Swiss-Prot
Match: GLNA3_MEDSA (Glutamine synthetase nodule isozyme OS=Medicago sativa GN=GS1 PE=2 SV=1)

HSP 1 Score: 265.003 bits (676), Expect = 1.971e-70
Identity = 124/139 (89.21%), Postives = 132/139 (94.96%), Query Frame = -1 
Query:  155 RYILERITEIAGVVLSFDPKPIKGDWNGAGAHANYSTKSMRNDGGIDVIKKAIEKLGKRHGEHIAAYGEGNERRLTGRHETADINTFSWGVANRGASIRVGRDTEKEGKGYFEDRRPASNMDPYVVTSMIAETTILWKP 571
            RYILERITEIAGVV+SFDPKPI GDWNGAGAH NYSTKSMR DGG ++IKKAIEKLG RH EHIAAYGEGNERRLTG+HET DINTFSWGVANRGAS+RVGRDTEK+GKGYFEDRRP+SNMDPYVVTSMIAETT+LWKP
Sbjct:  218 RYILERITEIAGVVVSFDPKPIPGDWNGAGAHTNYSTKSMREDGGYEIIKKAIEKLGLRHKEHIAAYGEGNERRLTGKHETTDINTFSWGVANRGASVRVGRDTEKDGKGYFEDRRPSSNMDPYVVTSMIAETTLLWKP 356
The following BLAST results are available for this feature:
BLAST of GE213218 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 89
Match NameE-valueIdentityDescription
GLNA1_VITVI2.106e-7294.24Glutamine synthetase cytosolic isozyme 1 OS=Vitis ... [more]
GLNA_VIGAC2.751e-7293.53Glutamine synthetase nodule isozyme OS=Vigna aconi... [more]
GLNA2_VITVI4.692e-7292.81Glutamine synthetase cytosolic isozyme 2 OS=Vitis ... [more]
GLNA1_LOTJA1.045e-7192.81Glutamine synthetase cytosolic isozyme OS=Lotus ja... [more]
GLNA1_ALNGL1.365e-7192.09Glutamine synthetase OS=Alnus glutinosa GN=GLN1 PE... [more]
GLNA2_SOYBN3.972e-7192.81Glutamine synthetase cytosolic isozyme 2 OS=Glycin... [more]
GLNA1_PHAVU3.972e-7191.37Glutamine synthetase PR-1 OS=Phaseolus vulgaris PE... [more]
GLNA1_MEDSA6.776e-7191.37Glutamine synthetase cytosolic isozyme OS=Medicago... [more]
GLNA3_PHAVU1.509e-7091.37Glutamine synthetase N-1 OS=Phaseolus vulgaris GN=... [more]
GLNA3_MEDSA1.971e-7089.21Glutamine synthetase nodule isozyme OS=Medicago sa... [more]

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Properties
Property NameValue
Genbank description60 BH (somaclonal variated plants of Skaggs Bonanza navel orange) SSH library Citrus sinensis cDNA 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>GE213218 ID=GE213218; Name=GE213218; organism=Citrus sinensis; type=EST; length=589bp
ACAGTTTTGAAGGGCAAGGAACAAATTCAAACAACAGACTTAAATGCATA
GACAAATTATAAAAACATAAGAATTATTTTTGTGGTGGGTTCAATTTAAG
CTTTCATTTTCCCTCGATTTCTTCTTCGTCATCCATGCTCAAGTTTGTTT
TCTAAGGCTTCCAGAGAATGGTGGTTTCAGCGATCATTGAAGTCACAACA
TATGGGTCCATGTTTGAAGCTGGCCTTCTGTCTTCAAAGTATCCCTTTCC
TTCTTTCTCAGTGTCACGACCAACGCGAATGGAGGCACCTCTGTTAGCGA
CACCCCAAGAAAAAGTGTTGATATCTGCAGTTTCATGTCGACCTGTCAGT
CTTCTTTCGTTGCCCTCTCCATAAGCAGCAATGTGTTCCCCATGGCGTTT
CCCCAACTTTTCAATTGCCTTCTTGATCACATCAATTCCACCATCATTCC
TCATAGACTTGGTGCTGTAGTTGGCGTGTGCACCAGCACCATTCCAGTCA
CCCTTGATTGGTTTTGGATCAAATGAGAGAACAACTCCGGCAATTTCAGT
AATCCTCTCGAGTATGTACCTCGGCCGCGACCACGCTAA
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