EY753938

Overview
NameEY753938
Unique NameEY753938
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length874
Libraries
Library NameType
Sweet orange leaf, field plant Acdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of EY753938 vs. ExPASy Swiss-Prot
Match: GSTF1_ARATH (Glutathione S-transferase 1 OS=Arabidopsis thaliana GN=GST1 PE=1 SV=2)

HSP 1 Score: 211.075 bits (536), Expect = 4.448e-65
Identity = 105/173 (60.69%), Postives = 129/173 (74.57%), Query Frame = 3
Query:   45 MAGIKVHGSVFSTATQRVFASLYEKELEYELVPVDMKAGEHKKEAFLSLNPFGQVPVLEHGDQKLFESRAITQYIAQEFPDKGTQLTCPGKPIAPLLVWKEVEALQFDPPSSKLNWELVYKPMFGMTTDPAAVEENEAKLAKILDVYEARLSQSKYLACDSFTLVDLHHLPIL 563
            MAGIKV G   STAT+RV  +L+EK +++E V V++K GEHKKE F+  NPFG+VP  E GD K+FESRAITQYIA EF DKG  L   GK +A + +  E+E+ +FDP  SKL WE V KP++GMTTD   VEE EAKLAK+LDVYE RL +SKYLA D FTLVDLH +P++
Sbjct:    1 MAGIKVFGHPASTATRRVLIALHEKNVDFEFVHVELKDGEHKKEPFILRNPFGKVPAFEDGDFKIFESRAITQYIAHEFSDKGNNLLSTGKDMAIIAMGIEIESHEFDPVGSKLVWEQVLKPLYGMTTDKTVVEEEEAKLAKVLDVYEHRLGESKYLASDHFTLVDLHTIPVI 173          

HSP 2 Score: 58.9214 bits (141), Expect = 4.448e-65
Identity = 28/38 (73.68%), Postives = 29/38 (76.32%), Query Frame = 2
Query:  554 PHINLLLGTPVKKLFDARPHVSAWAADITSRPAWAKXL 667
            P I  LLGTP KKLFD RPHVSAW ADITSRP+  K L
Sbjct:  171 PVIQYLLGTPTKKLFDERPHVSAWVADITSRPSAQKVL 208          
BLAST of EY753938 vs. ExPASy Swiss-Prot
Match: GSTF6_ARATH (Glutathione S-transferase 6, chloroplastic OS=Arabidopsis thaliana GN=GST6 PE=1 SV=3)

HSP 1 Score: 220.705 bits (561), Expect = 7.544e-65
Identity = 112/175 (64.00%), Postives = 137/175 (78.29%), Query Frame = 3
Query:   45 MAGIKVHGSVFSTATQRVFASLYEKELEYELVPVDMKAGEHKKEAFLSLNPFGQVPVLEHGDQKLFESRAITQYIAQEFPDKGTQLTCPG--KPIAPLLVWKEVEALQFDPPSSKLNWELVYKPMFGMTTDPAAVEENEAKLAKILDVYEARLSQSKYLACDSFTLVDLHHLPIL 563
            MA IKVHG   STAT RV A+LYEK+L++EL+PVDM+AG HK+EA L+LNPFGQ+P LE GD  LFESRAITQY+A+E+ +KG +L      K  A   VW +VE  QFDP +SKL +E V+K MFGMTTDPAAV+E E KL K+LDVYEARL++S++LA DSFTL DLHHLP +
Sbjct:   49 MASIKVHGVPMSTATMRVLATLYEKDLQFELIPVDMRAGAHKQEAHLALNPFGQIPALEDGDLTLFESRAITQYLAEEYSEKGEKLISQDCKKVKATTNVWLQVEGQQFDPNASKLAFERVFKGMFGMTTDPAAVQELEGKLQKVLDVYEARLAKSEFLAGDSFTLADLHHLPAI 223          

HSP 2 Score: 48.521 bits (114), Expect = 7.544e-65
Identity = 22/38 (57.89%), Postives = 27/38 (71.05%), Query Frame = 2
Query:  554 PHINLLLGTPVKKLFDARPHVSAWAADITSRPAWAKXL 667
            P I+ LLGT  K LFD+RP VS W   I++RPAWAK +
Sbjct:  221 PAIHYLLGTDSKVLFDSRPKVSEWIKKISARPAWAKVI 258          
BLAST of EY753938 vs. ExPASy Swiss-Prot
Match: GSTF1_TOBAC (Glutathione S-transferase PARB OS=Nicotiana tabacum GN=PARB PE=2 SV=1)

HSP 1 Score: 217.238 bits (552), Expect = 7.563e-65
Identity = 108/169 (63.91%), Postives = 130/169 (76.92%), Query Frame = 3
Query:   54 IKVHGSVFSTATQRVFASLYEKELEYELVPVDMKAGEHKKEAFLSLNPFGQVPVLEHGDQKLFESRAITQYIAQEFPDKGTQLTCPG-KPIAPLLVWKEVEALQFDPPSSKLNWELVYKPMFGMTTDPAAVEENEAKLAKILDVYEARLSQSKYLACDSFTLVDLHHLP 557
            IKVHGS  STAT RV A L EKEL++E VPVDM +GEHKK  +LSLNPFGQVP  E GD KLFESRAITQYIA  + D G QL     K +  + VW EVE  +F+PP++KL WEL  KP+ GMTTD AAV+E+EA+L+K+LD+YE +L++SKYL  DSFTLVDLHH+P
Sbjct:    3 IKVHGSPMSTATMRVAACLIEKELDFEFVPVDMASGEHKKHPYLSLNPFGQVPAFEDGDLKLFESRAITQYIAHVYADNGYQLILQDPKKMPSMSVWMEVEGQKFEPPATKLTWELGIKPIIGMTTDDAAVKESEAQLSKVLDIYETQLAESKYLGGDSFTLVDLHHIP 171          

HSP 2 Score: 51.9878 bits (123), Expect = 7.563e-65
Identity = 22/38 (57.89%), Postives = 29/38 (76.32%), Query Frame = 2
Query:  554 PHINLLLGTPVKKLFDARPHVSAWAADITSRPAWAKXL 667
            P+I  L+ + VK++FD+RP VSAW ADI +RPAW K L
Sbjct:  171 PNIYYLMSSKVKEVFDSRPRVSAWCADILARPAWVKGL 208          
BLAST of EY753938 vs. ExPASy Swiss-Prot
Match: GSTF2_TOBAC (Glutathione S-transferase APIC OS=Nicotiana tabacum GN=APIC PE=2 SV=1)

HSP 1 Score: 213.386 bits (542), Expect = 3.718e-64
Identity = 107/169 (63.31%), Postives = 128/169 (75.74%), Query Frame = 3
Query:   54 IKVHGSVFSTATQRVFASLYEKELEYELVPVDMKAGEHKKEAFLSLNPFGQVPVLEHGDQKLFESRAITQYIAQEFPDKGTQLTCPGKPIAPLL-VWKEVEALQFDPPSSKLNWELVYKPMFGMTTDPAAVEENEAKLAKILDVYEARLSQSKYLACDSFTLVDLHHLP 557
            IKVHGS  STAT RV A L EK+L++ELVPVDM +GEHKK  +LSLNPFGQVP  E GD KLFESRAITQYIA  + D G QL        P++ VW EVE  +F+P +SKL WEL  KP+ GMTTD  AV+E+E +L+K+LD+YE RL++SKYL  DSFTLVDLHH+P
Sbjct:    3 IKVHGSPMSTATMRVAACLIEKDLDFELVPVDMVSGEHKKHPYLSLNPFGQVPAFEDGDLKLFESRAITQYIAHVYADNGYQLILQDPKKMPIMSVWMEVEGQKFEPHASKLTWELGIKPIIGMTTDDDAVKESEVQLSKVLDIYETRLAESKYLGGDSFTLVDLHHIP 171          

HSP 2 Score: 53.5286 bits (127), Expect = 3.718e-64
Identity = 23/38 (60.53%), Postives = 29/38 (76.32%), Query Frame = 2
Query:  554 PHINLLLGTPVKKLFDARPHVSAWAADITSRPAWAKXL 667
            P+I  L+ T VK++FD+RP VSAW ADI +RPAW K L
Sbjct:  171 PNIYYLMSTKVKEVFDSRPRVSAWCADILARPAWVKGL 208          
BLAST of EY753938 vs. ExPASy Swiss-Prot
Match: GST11_ARATH (Glutathione S-transferase 11 OS=Arabidopsis thaliana GN=GST11 PE=1 SV=3)

HSP 1 Score: 207.223 bits (526), Expect = 6.323e-64
Identity = 106/174 (60.92%), Postives = 129/174 (74.14%), Query Frame = 3
Query:   45 MAGIKVHGSVFSTATQRVFASLYEKELEYELVPVDMKAGEHKKEAFLSLNPFGQVPVLEHGDQKLFESRAITQYIAQEFPDKGTQLTCPG-KPIAPLLVWKEVEALQFDPPSSKLNWELVYKPMFGMTTDPAAVEENEAKLAKILDVYEARLSQSKYLACDSFTLVDLHHLPIL 563
            MAGIKV G   STAT+RV  +L+EK L++E V +++K GEHKKE F+  NPFG+VP  E GD KLFESRAITQYIA  + DKG QL   G K IA + +  E+E+ +FDP  SKL WE V KP++GMTTD   VEE EAKLAK+LDVYE RL +SKYLA D FTLVDLH +P++
Sbjct:    1 MAGIKVFGHPASTATRRVLIALHEKNLDFEFVHIELKDGEHKKEPFIFRNPFGKVPAFEDGDFKLFESRAITQYIAHFYSDKGNQLVSLGSKDIAGIAMGIEIESHEFDPVGSKLVWEQVLKPLYGMTTDKTVVEEEEAKLAKVLDVYEHRLGESKYLASDKFTLVDLHTIPVI 174          

HSP 2 Score: 58.9214 bits (141), Expect = 6.323e-64
Identity = 28/38 (73.68%), Postives = 29/38 (76.32%), Query Frame = 2
Query:  554 PHINLLLGTPVKKLFDARPHVSAWAADITSRPAWAKXL 667
            P I  LLGTP KKLFD RPHVSAW ADITSRP+  K L
Sbjct:  172 PVIQYLLGTPTKKLFDERPHVSAWVADITSRPSAKKVL 209          
BLAST of EY753938 vs. ExPASy Swiss-Prot
Match: GSTF_SILCU (Glutathione S-transferase OS=Silene cucubalus GN=GST PE=1 SV=3)

HSP 1 Score: 215.312 bits (547), Expect = 1.074e-63
Identity = 114/174 (65.52%), Postives = 138/174 (79.31%), Query Frame = 3
Query:   54 IKVHGSVFSTATQRVFASLYEKELEYELVPVDMKAGEHKKEAFLSLNPFGQVPVLEHGDQKLFESRAITQYIA--QEFPDKGTQLTCPGK-PIAPLLVWKEVEALQFDPPSSKLNWELVYKPMFGMTTDPAAVEENEAKLAKILDVYEARLSQSKYL-ACDSFTLVDLHHLPIL 563
            IKVHG+  STATQRV  +LYEK LE+E VP+DM AG HK+ ++L+LNPFGQVP LE G+ KLFESRAIT+Y+A   +  ++GT L    K  +A  LVW+EVEA QFDP +SKL WELV+K +FGM TD   VEENEAKLAK+LDVYEARL++S+YL A DSFTLVDLHHLP+L
Sbjct:    3 IKVHGNPRSTATQRVLVALYEKHLEFEFVPIDMGAGGHKQPSYLALNPFGQVPALEDGEIKLFESRAITKYLAYTHDHQNEGTSLIHKEKHEMAAQLVWEEVEAHQFDPVASKLAWELVFKGIFGMQTDTTVVEENEAKLAKVLDVYEARLTESEYLGANDSFTLVDLHHLPLL 176          

HSP 2 Score: 50.0618 bits (118), Expect = 1.074e-63
Identity = 22/39 (56.41%), Postives = 27/39 (69.23%), Query Frame = 2
Query:  554 PHINLLLGTPVKKLFDARPHVSAWAADITSRPAWAKXLA 670
            P +  L+GT VKKLF+ R HVSAW   I +RP+W K LA
Sbjct:  174 PLLGYLMGTQVKKLFEERAHVSAWCKKILARPSWEKTLA 212          
BLAST of EY753938 vs. ExPASy Swiss-Prot
Match: GSTF_HYOMU (Glutathione S-transferase OS=Hyoscyamus muticus PE=1 SV=1)

HSP 1 Score: 214.542 bits (545), Expect = 6.125e-55
Identity = 110/188 (58.51%), Postives = 133/188 (70.74%), Query Frame = 3
Query:   51 GIKVHGSVFSTATQRVFASLYEKELEYELVPVDMKAGEHKKEAFLSLNPFGQVPVLEHGDQKLFESRAITQYIAQEFPDKGTQLTC-PGKPIAPLLVWKEVEALQFDPPSSKLNWELVYKPMFGMTTDPAAVEENEAKLAKILDVYEARLSQSKYLACDSFTLVDLHHLPILICCWGRLSRSCLMHAP 611
            G+K+HG   S A  RV A+L EK+L++ELVPV+M+AG+HKKE F++LNPFGQVP  E GD KLFESRAITQYIA  + DKG QL     K +A + VW EVE+ +FDP +SKL +E+V KPM GM TD AAV ENE KL K+LDVYE+RL  SKYL  DSFTL DLHH P +    G   +S     P
Sbjct:    2 GMKLHGPAMSPAVMRVIATLKEKDLDFELVPVNMQAGDHKKEPFITLNPFGQVPAFEDGDLKLFESRAITQYIAHTYADKGNQLLANDPKKMAIMSVWMEVESQKFDPVASKLTFEIVIKPMLGMVTDDAAVAENEEKLGKVLDVYESRLKDSKYLGGDSFTLADLHHAPAMNYLMGTKVKSLFDSRP 189          
BLAST of EY753938 vs. ExPASy Swiss-Prot
Match: GST16_ARATH (Glutathione S-transferase 16 OS=Arabidopsis thaliana GN=GST16 PE=1 SV=1)

HSP 1 Score: 199.904 bits (507), Expect = 1.561e-50
Identity = 107/193 (55.44%), Postives = 133/193 (68.91%), Query Frame = 3
Query:   45 MAGIKVHGSVFSTATQRVFASLYEKELEYELVPVDMKAGEHKKEAFLSLNPFGQVPVLEHGDQKLFESRAITQYIAQEFPDKGTQ-LTCPGKPIAPLLVWK---EVEALQFDPPSSKLNWELVYKPMFGMTTDPAAVEENEAKLAKILDVYEARLSQSKYLACDSFTLVDLHHLPILICCWGRLSRSCLMHAP 611
            MAGIKV G   ST+T+RV  +L+EK L++ELV V++K GEHKKE FLS NPFGQVP  E GD KLFESRAITQYIA  + ++GT  L    K IA   +     +VEA QFDP +SKL WE V+K  +G+ TD A V E EAKLAK+LDVYEARL + KYLA ++FTL DLHH+P++    G  ++      P
Sbjct:    1 MAGIKVFGHPASTSTRRVLIALHEKNLDFELVHVELKDGEHKKEPFLSRNPFGQVPAFEDGDLKLFESRAITQYIAHRYENQGTNLLPADSKNIAQYAIMSIGIQVEAHQFDPVASKLAWEQVFKFNYGLNTDQAVVAEEEAKLAKVLDVYEARLKEFKYLAGETFTLTDLHHIPVIQYLLGTPTKKLFTERP 193          
BLAST of EY753938 vs. ExPASy Swiss-Prot
Match: GSTF4_ARATH (Glutathione S-transferase PM24 OS=Arabidopsis thaliana GN=PM24.1 PE=1 SV=3)

HSP 1 Score: 194.126 bits (492), Expect = 8.567e-49
Identity = 105/193 (54.40%), Postives = 133/193 (68.91%), Query Frame = 3
Query:   45 MAGIKVHGSVFSTATQRVFASLYEKELEYELVPVDMKAGEHKKEAFLSLNPFGQVPVLEHGDQKLFESRAITQYIAQEFPDKGTQ-LTCPGKPI---APLLVWKEVEALQFDPPSSKLNWELVYKPMFGMTTDPAAVEENEAKLAKILDVYEARLSQSKYLACDSFTLVDLHHLPILICCWGRLSRSCLMHAP 611
            MAGIKV G   S AT+RV  +L+EK L++ELV V++K GEHKKE FLS NPFGQVP  E GD KLFESRAITQYIA  + ++GT  L    K I   A + +  +VE  QFDP +SKL +E ++K ++G+TTD A V E EAKLAK+LDVYEARL + KYLA ++FTL DLHH+P +    G  ++      P
Sbjct:    1 MAGIKVFGHPASIATRRVLIALHEKNLDFELVHVELKDGEHKKEPFLSRNPFGQVPAFEDGDLKLFESRAITQYIAHRYENQGTNLLQTDSKNISQYAIMAIGMQVEDHQFDPVASKLAFEQIFKSIYGLTTDEAVVAEEEAKLAKVLDVYEARLKEFKYLAGETFTLTDLHHIPAIQYLLGTPTKKLFTERP 193          
BLAST of EY753938 vs. ExPASy Swiss-Prot
Match: GSTF3_MAIZE (Glutathione S-transferase 3 OS=Zea mays GN=GST3 PE=1 SV=4)

HSP 1 Score: 159.458 bits (402), Expect = 1.048e-41
Identity = 85/175 (48.57%), Postives = 115/175 (65.71%), Query Frame = 3
Query:   45 MAGIKVHGSVFSTATQRVFASLYEKELEYELVPVDMKAGEHKKEAFLSLNPFGQVPVLEHGDQKLFESRAITQYIAQEFPDKGTQLTCPGKPIAPLLVWKEVEALQFDPPSSKLNWELVYKPMFGMTTDPAAVEENEAKLAKILDVYEARLSQSKYLACDSFTLVDLHH--LPIL 563
            MA +K++G   S    RV   L EK L++E+VPVD+  G HK+  FL+LNPFGQ+P L  GD+ LFESRAI +YIA ++  +GT L       A L VW EVE+  F P +S L ++L+ +P+ G   D A VE++  +LAK+LDVYEA L+++KYLA D FTL D +H  LP L
Sbjct:    1 MAPLKLYGMPLSPNVVRVATVLNEKGLDFEIVPVDLTTGAHKQPDFLALNPFGQIPALVDGDEVLFESRAINRYIASKYASEGTDLLPATASAAKLEVWLEVESHHFHPNASPLVFQLLVRPLLGGAPDAAVVEKHAEQLAKVLDVYEAHLARNKYLAGDEFTLADANHALLPAL 175          

HSP 2 Score: 32.3426 bits (72), Expect = 1.048e-41
Identity = 17/40 (42.50%), Postives = 23/40 (57.50%), Query Frame = 2
Query:  602 ARPHVSAWAADITSRPAWAKXLACSTLNTLPVPTXRSRSS 721
            ARPHV AW   I +RPA+ K     T+  +P+P   S S+
Sbjct:  188 ARPHVKAWWEAIAARPAFQK-----TVAAIPLPPPPSSSA 222          
The following BLAST results are available for this feature:
BLAST of EY753938 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 19
Match NameE-valueIdentityDescription
GSTF1_ARATH4.448e-6560.69Glutathione S-transferase 1 OS=Arabidopsis thalian... [more]
GSTF6_ARATH7.544e-6564.00Glutathione S-transferase 6, chloroplastic OS=Arab... [more]
GSTF1_TOBAC7.563e-6563.91Glutathione S-transferase PARB OS=Nicotiana tabacu... [more]
GSTF2_TOBAC3.718e-6463.31Glutathione S-transferase APIC OS=Nicotiana tabacu... [more]
GST11_ARATH6.323e-6460.92Glutathione S-transferase 11 OS=Arabidopsis thalia... [more]
GSTF_SILCU1.074e-6365.52Glutathione S-transferase OS=Silene cucubalus GN=G... [more]
GSTF_HYOMU6.125e-5558.51Glutathione S-transferase OS=Hyoscyamus muticus PE... [more]
GST16_ARATH1.561e-5055.44Glutathione S-transferase 16 OS=Arabidopsis thalia... [more]
GSTF4_ARATH8.567e-4954.40Glutathione S-transferase PM24 OS=Arabidopsis thal... [more]
GSTF3_MAIZE1.048e-4148.57Glutathione S-transferase 3 OS=Zea mays GN=GST3 PE... [more]

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Properties
Property NameValue
Genbank descriptionCS12-C1-001-008-F07-CT.F Sweet orange leaf, field plant A Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY753938 ID=EY753938; Name=EY753938; organism=Citrus sinensis; type=EST; length=874bp
AAGTCACAACTTACAATAACAAGCGAGGGAAGGAAGACCCAAANATGGCA
GGCATCAAGGTCCACGGTTCTGTTTTCTCAACTGCTACGCAGCGAGTTTT
CGCATCCCTCTACGAGAAGGAGCTCGAATACGAGCTTGTCCCCGTAGACA
TGAAGGCTGGTGAACACAAAAAGGAGGCCTTCCTTTCTTTGAATCCATTC
GGTCAAGTTCCGGTGCTTGAACACGGCGACCAGAAGCTCTTCGAATCAAG
GGCAATCACCCAATACATCGCCCAGGAGTTTCCAGACAAAGGCACCCAGT
TAACATGCCCAGGCAAGCCCATCGCCCCGTTATTGGTATGGAAGGAGGTG
GAGGCACTCCAGTTCGACCCCCCATCATCGAAGCTCAACTGGGAGCTCGT
GTACAAGCCAATGTTTGGAATGACCACTGATCCAGCTGCGGTTGAAGAAA
ATGAAGCTAAGCTTGCCAAAATTTTAGATGTTTACGAGGCCCGTTTGTCT
CAGTCCAAATACTTGGCGTGCGACAGCTTCACCTTGGTCGATTTGCATCA
TCTCCCCATATTAATTTGTTGTTGGGGACGCCTGTCAAGAAGCTGTTTGA
TGCACGCCCCCATGTTAGCGCATGGGCAGCTGATATCACTTCCAGACCGG
CTTGGGCTAAGGNCCTTGCCTGCTCAACACTGAACACTCTGCCCGTACCT
ACTANACGTAGTCGCTCCTCGGGGGCACTTATCTTTGTATACCTATTAGG
CTGGNTTTTCAGTTCGTATTCTCTGTTGGGCGTTTGTCATCATATTCTGC
AAATATTGATGCCTTGAATTTGACTACCGCTTTGATTTAACGGACTGCCC
TATTAACTTGGAGTGAGGGCTGAG
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