EY753657

Overview
NameEY753657
Unique NameEY753657
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length943
Libraries
Library NameType
Sweet orange leaf, field plant Acdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
EY753657_ssr25 microsatellite EY753657_ssr25:25..36. BLAST: Citrus ESTs to Prunus persica proteins V1
Homology
[Close]
BLAST of EY753657 vs. ExPASy Swiss-Prot
Match: DFRA_MALDO (Bifunctional dihydroflavonol 4-reductase/flavanone 4-reductase OS=Malus domestica GN=DFR PE=1 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 1.049e-25
Identity = 60/132 (45.45%), Postives = 86/132 (65.15%), Query Frame = 2 
Query:  131 EEEAVCVTGANGFIGTWLVKTLLDNNYTSINATVFPGSDS---SHLFALPGAGDANLRVFEADVLDSGAVSRAVEGCKGVFHVASPCTLEDPVDPEKELILPAVQGTLNVLEAA-KRFGVRRVVVTSSISAI 514
            E E+VCVTGA+GFIG+WLV  LL++ YT + ATV   ++     HL  LP A + +L +++AD+ D G+   A++GC GVFHVA+P   E   DPE E+I P + G L++L+A  K   VR++V TSS   +
Sbjct:    4 ESESVCVTGASGFIGSWLVMRLLEHGYT-VRATVRDPTNQKKVKHLLDLPKA-ETHLTLWKADLADEGSFDEAIQGCSGVFHVATPMDFESK-DPENEVIKPTINGLLDILKACQKAKTVRKLVFTSSAGTV 132

HSP 2 Score: 28.1054 bits (61), Expect = 1.049e-25
Identity = 15/27 (55.56%), Postives = 21/27 (77.78%), Query Frame = 3 
Query:  576 CKSRKK--W-YPVSKTLAKKAAWEFAE 647
            C+S K   W Y VSKTLA++AAW++A+
Sbjct:  153 CRSVKMTGWMYFVSKTLAEQAAWKYAK 179

HSP 3 Score: 23.0978 bits (48), Expect = 1.049e-25
Identity = 6/15 (40.00%), Postives = 13/15 (86.67%), Query Frame = 1 
Query:  532 ESKVFDETSWTDLEY 576
            +  V+DE++W+D+E+
Sbjct:  138 QKPVYDESNWSDVEF 152
[Close]
BLAST of EY753657 vs. ExPASy Swiss-Prot
Match: DFRA_PYRCO (Bifunctional dihydroflavonol 4-reductase/flavanone 4-reductase OS=Pyrus communis GN=DFR PE=1 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 1.362e-25
Identity = 60/132 (45.45%), Postives = 86/132 (65.15%), Query Frame = 2 
Query:  131 EEEAVCVTGANGFIGTWLVKTLLDNNYTSINATVFPGSDS---SHLFALPGAGDANLRVFEADVLDSGAVSRAVEGCKGVFHVASPCTLEDPVDPEKELILPAVQGTLNVLEAA-KRFGVRRVVVTSSISAI 514
            E E+VCVTGA+GFIG+WLV  LL++ YT + ATV   ++     HL  LP A + +L +++AD+ D G+   A++GC GVFHVA+P   E   DPE E+I P + G L++L+A  K   VR++V TSS   +
Sbjct:    4 ESESVCVTGASGFIGSWLVMRLLEHGYT-VRATVRDPTNQKKVKHLLDLPKA-ETHLTLWKADLADEGSFDEAIQGCSGVFHVATPMDFESR-DPENEVIKPTINGLLDILKACQKAKTVRKLVFTSSAGTV 132

HSP 2 Score: 28.1054 bits (61), Expect = 1.362e-25
Identity = 15/27 (55.56%), Postives = 21/27 (77.78%), Query Frame = 3 
Query:  576 CKSRKK--W-YPVSKTLAKKAAWEFAE 647
            C+S K   W Y VSKTLA++AAW++A+
Sbjct:  153 CRSVKMTGWMYFVSKTLAEQAAWKYAK 179

HSP 3 Score: 23.0978 bits (48), Expect = 1.362e-25
Identity = 6/15 (40.00%), Postives = 13/15 (86.67%), Query Frame = 1 
Query:  532 ESKVFDETSWTDLEY 576
            +  V+DE++W+D+E+
Sbjct:  138 QKPVYDESNWSDVEF 152
[Close]
BLAST of EY753657 vs. ExPASy Swiss-Prot
Match: DFRA_ARATH (Dihydroflavonol-4-reductase OS=Arabidopsis thaliana GN=DFRA PE=2 SV=2)

HSP 1 Score: 96.2857 bits (238), Expect = 1.090e-24
Identity = 60/132 (45.45%), Postives = 80/132 (60.61%), Query Frame = 2 
Query:  131 EEEAVCVTGANGFIGTWLVKTLLDNNYTSINATV-FPGS--DSSHLFALPGAGDANLRVFEADVLDSGAVSRAVEGCKGVFHVASPCTLEDPVDPEKELILPAVQGTLNVLEA-AKRFGVRRVVVTSSISAI 514
            ++E VCVTGA+GFIG+WLV  LL+  Y  + ATV  PG+     HL  LP A    L +++AD+ + G+   A+ GC GVFHVA+P   E   DPE E+I P V G L +++A  K   VRR V TSS   +
Sbjct:    4 QKETVCVTGASGFIGSWLVMRLLERGY-FVRATVRDPGNLKKVQHLLDLPNA-KTLLTLWKADLSEEGSYDDAINGCDGVFHVATPMDFESK-DPENEVIKPTVNGMLGIMKACVKAKTVRRFVFTSSAGTV 132

HSP 2 Score: 33.8834 bits (76), Expect = 1.090e-24
Identity = 15/20 (75.00%), Postives = 17/20 (85.00%), Query Frame = 3 
Query:  597 YPVSKTLAKKAAWEFAENMG 656
            Y VSKTLA+KAAW+FAE  G
Sbjct:  163 YFVSKTLAEKAAWDFAEEKG 182

HSP 3 Score: 23.8682 bits (50), Expect = 1.090e-24
Identity = 7/15 (46.67%), Postives = 11/15 (73.33%), Query Frame = 1 
Query:  532 ESKVFDETSWTDLEY 576
            +  V+DE  W+DLE+
Sbjct:  138 QKNVYDENDWSDLEF 152
[Close]
BLAST of EY753657 vs. ExPASy Swiss-Prot
Match: DFRA_DIACA (Dihydroflavonol-4-reductase OS=Dianthus caryophyllus GN=A PE=2 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 1.137e-23
Identity = 57/125 (45.60%), Postives = 81/125 (64.80%), Query Frame = 2 
Query:  137 EAVCVTGANGFIGTWLVKTLLDNNYTSINATVFPGSDS---SHLFALPGAGDANLRVFEADVLDSGAVSRAVEGCKGVFHVASPCTLEDPVDPEKELILPAVQGTLNVLEAAKRFGVRRVVVTSS 502
            E VCVTGA+GFIG+WL+  LL+  YT + ATV    ++    HL  LP A   NL +++AD+ + G+   AV+GC GVFH+A+P   E   DPE E+I P + G L++L++  +  +RRVV TSS
Sbjct:   23 ETVCVTGASGFIGSWLIMRLLERGYT-VRATVRDPDNTKKVQHLLDLPNA-KTNLTLWKADLHEEGSFDAAVDGCTGVFHIATPMDFESK-DPENEMIKPTINGMLDILKSCVKAKLRRVVFTSS 144

HSP 2 Score: 24.6386 bits (52), Expect = 1.137e-23
Identity = 10/16 (62.50%), Postives = 14/16 (87.50%), Query Frame = 3 
Query:  597 YPVSKTLAKKAAWEFA 644
            Y VSK LA++AAW++A
Sbjct:  179 YFVSKILAEQAAWKYA 194

HSP 3 Score: 21.1718 bits (43), Expect = 1.137e-23
Identity = 7/23 (30.43%), Postives = 12/23 (52.17%), Query Frame = 1 
Query:  508 GNCTESRLESKVFDETSWTDLEY 576
            G       +  V+DET W+ L++
Sbjct:  146 GTVNVEATQKPVYDETCWSALDF 168
[Close]
BLAST of EY753657 vs. ExPASy Swiss-Prot
Match: DFRA_VITVI (Dihydroflavonol-4-reductase OS=Vitis vinifera GN=DFR PE=1 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 1.478e-23
Identity = 57/132 (43.18%), Postives = 83/132 (62.88%), Query Frame = 2 
Query:  131 EEEAVCVTGANGFIGTWLVKTLLDNNYTSINATVFPGSDSS---HLFALPGAGDANLRVFEADVLDSGAVSRAVEGCKGVFHVASPCTLEDPVDPEKELILPAVQGTLNVLEA-AKRFGVRRVVVTSSISAI 514
            + E VCVTGA+GFIG+WLV  LL+   T + ATV   ++     HL  LP A + +L +++AD+ D G+   A++GC GVFHVA+P   E   DPE E+I P ++G L ++++ A    VRR+V TSS   +
Sbjct:    4 QSETVCVTGASGFIGSWLVMRLLERRLT-VRATVRDPTNVKKVKHLLDLPKA-ETHLTLWKADLADEGSFDEAIKGCTGVFHVATPMDFESK-DPENEVIKPTIEGMLGIMKSCAAAKTVRRLVFTSSAGTV 132

HSP 2 Score: 28.8758 bits (63), Expect = 1.478e-23
Identity = 14/27 (51.85%), Postives = 22/27 (81.48%), Query Frame = 3 
Query:  576 CKSRKK--W-YPVSKTLAKKAAWEFAE 647
            C+++K   W Y VSKTLA++AAW++A+
Sbjct:  153 CRAKKMTAWMYFVSKTLAEQAAWKYAK 179

HSP 3 Score: 22.7126 bits (47), Expect = 1.478e-23
Identity = 6/17 (35.29%), Postives = 13/17 (76.47%), Query Frame = 1 
Query:  541 VFDETSWTDLEYASLER 591
            V+DE+ W+D+E+   ++
Sbjct:  141 VYDESCWSDMEFCRAKK 157
[Close]
BLAST of EY753657 vs. ExPASy Swiss-Prot
Match: DFRA_CALCH (Dihydroflavonol-4-reductase OS=Callistephus chinensis GN=F PE=2 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 3.338e-22
Identity = 57/128 (44.53%), Postives = 79/128 (61.72%), Query Frame = 2 
Query:  143 VCVTGANGFIGTWLVKTLLDNNYTSINATVFPGSDSS---HLFALPGAGDANLRVFEADVLDSGAVSRAVEGCKGVFHVASPCTLEDPVDPEKELILPAVQGTLNVLEA-AKRFGVRRVVVTSSISAI 514
            VCVTGA GFIG+WLV  LL+  Y  + ATV    D     HL  LP A + NL +++AD+   G+   A+EGC GVFHVA+P   E   DPE E+I P ++G L+++ + AK   V+++V TSS   +
Sbjct:    9 VCVTGAAGFIGSWLVMRLLERGYI-VRATVRNPGDMKKVKHLLELPKA-ETNLTLWKADLTQEGSFDEAIEGCHGVFHVATPMDFESK-DPENEIIKPTIEGILSIIRSCAKAKTVKKLVYTSSAGTV 133

HSP 2 Score: 24.2534 bits (51), Expect = 3.338e-22
Identity = 14/24 (58.33%), Postives = 16/24 (66.67%), Query Frame = 3 
Query:  579 KSRKKW-YPVSKTLAKKAAWEFAE 647
            K    W Y VSKTLA+KAA E A+
Sbjct:  157 KKMTAWMYFVSKTLAEKAAMEAAK 180

HSP 3 Score: 22.3274 bits (46), Expect = 3.338e-22
Identity = 9/22 (40.91%), Postives = 16/22 (72.73%), Query Frame = 1 
Query:  511 NCTESRLESKVFDETSWTDLEY 576
            N  E++L   V+DE+ W+DL++
Sbjct:  134 NVQETQLP--VYDESHWSDLDF 153
[Close]
BLAST of EY753657 vs. ExPASy Swiss-Prot
Match: DFRA_GERHY (Dihydroflavonol-4-reductase OS=Gerbera hybrida GN=DFR PE=2 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 4.327e-22
Identity = 54/128 (42.19%), Postives = 79/128 (61.72%), Query Frame = 2 
Query:  143 VCVTGANGFIGTWLVKTLLDNNYTSINATVFPGSDSS---HLFALPGAGDANLRVFEADVLDSGAVSRAVEGCKGVFHVASPCTLEDPVDPEKELILPAVQGTLNVLEA-AKRFGVRRVVVTSSISAI 514
            VCVTGA GFIG+WLV  LL+  Y  ++ATV    D     HL  LP A   NL++++AD+   G+   A++GC GVFH+A+P   E   DPE E+I P ++G L+++ +  K   V+++V TSS   +
Sbjct:    9 VCVTGAAGFIGSWLVMRLLERGYV-VHATVRDPGDLKKVKHLLELPKA-QTNLKLWKADLTQEGSFDEAIQGCHGVFHLATPMDFESK-DPENEIIKPTIEGVLSIIRSCVKAKTVKKLVFTSSAGTV 133

HSP 2 Score: 26.5646 bits (57), Expect = 4.327e-22
Identity = 13/21 (61.90%), Postives = 15/21 (71.43%), Query Frame = 3 
Query:  579 KSRKKW-YPVSKTLAKKAAWE 638
            K    W Y VSKTLA+KAAW+
Sbjct:  157 KKMTAWMYFVSKTLAEKAAWD 177

HSP 3 Score: 21.9422 bits (45), Expect = 4.327e-22
Identity = 6/12 (50.00%), Postives = 11/12 (91.67%), Query Frame = 1 
Query:  541 VFDETSWTDLEY 576
            V+DE+ W+DL++
Sbjct:  142 VYDESHWSDLDF 153
[Close]
BLAST of EY753657 vs. ExPASy Swiss-Prot
Match: DFRA_PETHY (Dihydroflavonol-4-reductase OS=Petunia hybrida GN=DFRA PE=2 SV=2)

HSP 1 Score: 101.293 bits (251), Expect = 1.016e-21
Identity = 59/128 (46.09%), Postives = 83/128 (64.84%), Query Frame = 2 
Query:  143 VCVTGANGFIGTWLVKTLLDNNYTSINATVFPGSDSS---HLFALPGAGDANLRVFEADVLDSGAVSRAVEGCKGVFHVASPCTLEDPVDPEKELILPAVQGTLNVLEA-AKRFGVRRVVVTSSISAI 514
            VCVTGA GFIG+WLV  LL+  Y +++ATV    +     HL  LP A D NL +++AD+   G+   A++GC+GVFHVA+P   E   DPE E+I P V+G L+++E+ AK   V+R+V TSS   +
Sbjct:   18 VCVTGAAGFIGSWLVMRLLERGY-NVHATVRDPENKKKVKHLLELPKA-DTNLTLWKADLTVEGSFDEAIQGCQGVFHVATPMDFESK-DPENEVIKPTVRGMLSIIESCAKANTVKRLVFTSSAGTL 142

HSP 2 Score: 22.7126 bits (47), Expect = 1.016e-21
Identity = 6/11 (54.55%), Postives = 11/11 (100.00%), Query Frame = 1 
Query:  544 FDETSWTDLEY 576
            +D+TSW+DL++
Sbjct:  152 YDQTSWSDLDF 162
[Close]
BLAST of EY753657 vs. ExPASy Swiss-Prot
Match: DFRA_MAIZE (Dihydroflavonol-4-reductase OS=Zea mays GN=A1 PE=3 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 3.458e-21
Identity = 56/132 (42.42%), Postives = 81/132 (61.36%), Query Frame = 2 
Query:  131 EEEAVCVTGANGFIGTWLVKTLLDNNYTSINATVFPGSD---SSHLFALPGAGDANLRVFEADVLDSGAVSRAVEGCKGVFHVASPCTLEDPVDPEKELILPAVQGTLNVLEAAKRFG-VRRVVVTSSISAI 514
            E+  V VTGA+GF+G+WLV  LL   YT + ATV   ++   +  L  LPGA +  L +++AD+ + G+   A+ GC GVFHVA+P       DPE E+I P V+G ++++ A K  G VRR+V TSS   +
Sbjct:    9 EKGTVLVTGASGFVGSWLVMKLLQAGYT-VRATVRDPANVGKTKPLMDLPGATE-RLSIWKADLAEEGSFHDAIRGCTGVFHVATPMDFLSK-DPENEVIKPTVEGMISIMRACKEAGTVRRIVFTSSAGTV 137

HSP 2 Score: 23.8682 bits (50), Expect = 3.458e-21
Identity = 12/20 (60.00%), Postives = 14/20 (70.00%), Query Frame = 3 
Query:  597 YPVSKTLAKKAAWEFAENMG 656
            Y VSKTLA+KAA  +A   G
Sbjct:  168 YFVSKTLAEKAALAYAAEHG 187

HSP 3 Score: 23.483 bits (49), Expect = 3.458e-21
Identity = 7/12 (58.33%), Postives = 11/12 (91.67%), Query Frame = 1 
Query:  541 VFDETSWTDLEY 576
            V+DE SWTD+++
Sbjct:  146 VYDEESWTDVDF 157
[Close]
BLAST of EY753657 vs. ExPASy Swiss-Prot
Match: DFRA_HORVU (Dihydroflavonol-4-reductase OS=Hordeum vulgare GN=ANT18 PE=3 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 3.789e-21
Identity = 56/128 (43.75%), Postives = 82/128 (64.06%), Query Frame = 2 
Query:  143 VCVTGANGFIGTWLVKTLLDNNYTSINATVFPGSD---SSHLFALPGAGDANLRVFEADVLDSGAVSRAVEGCKGVFHVASPCTLEDPVDPEKELILPAVQGTLNVLEAAKRFG-VRRVVVTSSISAI 514
            V VTGA+GF+G+WLV  LL   YT + ATV   ++   +  L  LPGA +  L +++AD+ + G+ + A+ GC GVFHVA+P    D  DPE E+I P V+G L+++ A K  G V+R+V TSS  ++
Sbjct:    8 VVVTGASGFVGSWLVMKLLQAGYT-VRATVRDPANVEKTKPLLELPGAKE-RLSIWKADLSEDGSFNEAIAGCTGVFHVATPMDF-DSQDPENEVIKPTVEGMLSIMRACKEAGTVKRIVFTSSAGSV 132

HSP 2 Score: 25.0238 bits (53), Expect = 3.789e-21
Identity = 12/20 (60.00%), Postives = 14/20 (70.00%), Query Frame = 3 
Query:  597 YPVSKTLAKKAAWEFAENMG 656
            Y VSK LA+KAA E+A   G
Sbjct:  163 YFVSKALAEKAAMEYASENG 182
The following BLAST results are available for this feature:
BLAST of EY753657 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 16
Match NameE-valueIdentityDescription
DFRA_MALDO1.049e-2545.45Bifunctional dihydroflavonol 4-reductase/flavanone... [more]
DFRA_PYRCO1.362e-2545.45Bifunctional dihydroflavonol 4-reductase/flavanone... [more]
DFRA_ARATH1.090e-2445.45Dihydroflavonol-4-reductase OS=Arabidopsis thalian... [more]
DFRA_DIACA1.137e-2345.60Dihydroflavonol-4-reductase OS=Dianthus caryophyll... [more]
DFRA_VITVI1.478e-2343.18Dihydroflavonol-4-reductase OS=Vitis vinifera GN=D... [more]
DFRA_CALCH3.338e-2244.53Dihydroflavonol-4-reductase OS=Callistephus chinen... [more]
DFRA_GERHY4.327e-2242.19Dihydroflavonol-4-reductase OS=Gerbera hybrida GN=... [more]
DFRA_PETHY1.016e-2146.09Dihydroflavonol-4-reductase OS=Petunia hybrida GN=... [more]
DFRA_MAIZE3.458e-2142.42Dihydroflavonol-4-reductase OS=Zea mays GN=A1 PE=3... [more]
DFRA_HORVU3.789e-2143.75Dihydroflavonol-4-reductase OS=Hordeum vulgare GN=... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionCS12-C1-001-005-D09-CT.F Sweet orange leaf, field plant A Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY753657 ID=EY753657; Name=EY753657; organism=Citrus sinensis; type=EST; length=943bp
AAACATTATTTTAATCCATCCAGTTCATCATCATCATATCCTCTGACTAT
AAAATCCCAATCACTGTAATCCCGACAGCTCAAGTCTAATTTTTTTTTTC
TTTTACAGAATGGCGTCAGAAGCCAAAAAGGAAGAAGAAGCAGTGTGCGT
GACCGGAGCCAACGGGTTCATCGGCACGTGGCTAGTCAAGACCCTTTTAG
ACAACAACTACACTTCCATAAACGCCACCGTTTTCCCCGGCTCCGACTCA
TCTCACTTGTTCGCCCTCCCTGGCGCCGGCGATGCCAACTTGCGAGTGTT
CGAGGCCGACGTGTTGGACTCCGGCGCCGTCTCGAGGGCGGTTGAGGGAT
GCAAGGGGGTATTCCACGTGGCGTCTCCTTGTACACTGGAGGATCCTGTG
GACCCCGAGAAGGAGCTCATTCTGCCGGCGGTTCAAGGGACGCTTAATGT
GCTTGAGGCTGCTAAAAGGTTTGGGGTGAGGCGCGTGGTGGTCACCAGCT
CCATTTCGGCAATTGTACCGAATCCCGGCTGGAAAGTAAAGTCTTCGATG
AGACTTCGTGGACTGATCTTGAATATGCAAGTCTCGAAAGAAGTGGTATC
CAGTGTCAAAGACATTGGCAAAGAAAGCTGCATGGGAGTTTGCTGAAAAC
ATGGGTTGGATTGGTGGGGGCCATCCATCCTGCTACATGTTTGGGGGCCC
TCATGCAGCCTTATTAAACGCGAGCTGTCCTGGACTACAACAATTGTTAC
AGGGGTTCAAAGAAACTCAAGAGTTCACCTGGTTGGGGAGCCGTTCATGT
TAAAAATGTACCAAGGGCGCAGGGGTGGTTTTTGAAATCCCCGCTGCGTC
GGGGAAAATCTGGGCCCAAAGGTATCTTTCAGGTGGGGGCTTTTGCTGAA
AAGATCTTAAACCTTCCCTTGAATTCCATAACAAGGTTCGATG
back to top