EY724975

Overview
NameEY724975
Unique NameEY724975
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length869
Libraries
Library NameType
Sweet orange fruit, development stadium (4 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY724975 vs. ExPASy Swiss-Prot
Match: PRS8A_ARATH (26S protease regulatory subunit 8 homolog A OS=Arabidopsis thaliana GN=RPT6A PE=2 SV=1)

HSP 1 Score: 320.857 bits (821), Expect = 8.185e-90
Identity = 165/197 (83.76%), Postives = 178/197 (90.36%), Query Frame = 2 
Query:   77 MAAVGIETKQPESAAPEEVCAAKGAS-KQGEGLRQYYLQHIHELQLQVRQKTHNLNRLEAQRNELNSRVRMLKEELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPR 664
            MAAVG+++++PE+A  EE C  KGA+ KQGEGL+QYYLQHIHELQ Q+RQKT+NLNRLEAQRNELNSRVRML+EELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVV            PSTRVALRNDSYVLHL+LPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQP+
Sbjct:    1 MAAVGVDSRRPETAM-EETCNVKGAAAKQGEGLKQYYLQHIHELQRQLRQKTNNLNRLEAQRNELNSRVRMLREELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVDIDKSIDITKITPSTRVALRNDSYVLHLVLPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPK 196

HSP 2 Score: 31.9574 bits (71), Expect = 8.185e-90
Identity = 14/17 (82.35%), Postives = 14/17 (82.35%), Query Frame = 1 
Query:  661 KGVLLYGPPATGTTLWA 711
            KGVLLYGPP TG TL A
Sbjct:  196 KGVLLYGPPGTGKTLLA 212
[Close]
BLAST of EY724975 vs. ExPASy Swiss-Prot
Match: PRS8B_ARATH (26S protease regulatory subunit 8 homolog B OS=Arabidopsis thaliana GN=RPT6B PE=2 SV=1)

HSP 1 Score: 305.449 bits (781), Expect = 3.391e-85
Identity = 159/197 (80.71%), Postives = 171/197 (86.80%), Query Frame = 2 
Query:   77 MAAVGIETKQPESAAPEEVCAAKGAS-KQGEGLRQYYLQHIHELQLQVRQKTHNLNRLEAQRNELNSRVRMLKEELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPR 664
            MAAVG+E + P +A  EE C  KGA+ KQGEGL +YYLQH+ ELQ   R+K++NLNRLEAQRNELNSRVRML+EELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVV            PSTRVALRNDSYVLHL+LPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQP+
Sbjct:    1 MAAVGVEARPPVTAM-EETCNVKGAAAKQGEGLNKYYLQHLDELQRLQREKSYNLNRLEAQRNELNSRVRMLREELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVDIDKSIDITKLTPSTRVALRNDSYVLHLVLPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPK 196

HSP 2 Score: 31.9574 bits (71), Expect = 3.391e-85
Identity = 14/17 (82.35%), Postives = 14/17 (82.35%), Query Frame = 1 
Query:  661 KGVLLYGPPATGTTLWA 711
            KGVLLYGPP TG TL A
Sbjct:  196 KGVLLYGPPGTGKTLLA 212
[Close]
BLAST of EY724975 vs. ExPASy Swiss-Prot
Match: PRS8_RAT (26S protease regulatory subunit 8 OS=Rattus norvegicus GN=Psmc5 PE=1 SV=1)

HSP 1 Score: 246.899 bits (629), Expect = 1.162e-67
Identity = 127/181 (70.17%), Postives = 146/181 (80.66%), Query Frame = 2 
Query:  122 PEEVCAAKGASKQGEGLRQYYLQHIHELQLQVRQKTHNLNRLEAQRNELNSRVRMLKEELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPR 664
            PE++   +G  K G GLRQYYL  I ELQL V  K+ NL RL+AQRNELN++VR+L+EELQLLQE GSYVGEVV+ M K KVLVKVHPEGK+VV            P+ RVALRNDSY LH ILP+KVDPLV+LM VEKVPDSTY+MIGGLD+QIKEIKEVIELP+KHPELFE+LGIAQP+
Sbjct:    6 PEQMELEEG--KAGSGLRQYYLSKIEELQLIVNDKSQNLRRLQAQRNELNAKVRLLREELQLLQEQGSYVGEVVRAMDKKKVLVKVHPEGKFVVDVDKNIDINDVTPNCRVALRNDSYTLHKILPNKVDPLVSLMMVEKVPDSTYEMIGGLDKQIKEIKEVIELPVKHPELFEALGIAQPK 184

HSP 2 Score: 31.9574 bits (71), Expect = 1.162e-67
Identity = 14/17 (82.35%), Postives = 14/17 (82.35%), Query Frame = 1 
Query:  661 KGVLLYGPPATGTTLWA 711
            KGVLLYGPP TG TL A
Sbjct:  184 KGVLLYGPPGTGKTLLA 200
[Close]
BLAST of EY724975 vs. ExPASy Swiss-Prot
Match: PRS8_PIG (26S protease regulatory subunit 8 OS=Sus scrofa GN=PSMC5 PE=2 SV=1)

HSP 1 Score: 246.899 bits (629), Expect = 1.162e-67
Identity = 127/181 (70.17%), Postives = 146/181 (80.66%), Query Frame = 2 
Query:  122 PEEVCAAKGASKQGEGLRQYYLQHIHELQLQVRQKTHNLNRLEAQRNELNSRVRMLKEELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPR 664
            PE++   +G  K G GLRQYYL  I ELQL V  K+ NL RL+AQRNELN++VR+L+EELQLLQE GSYVGEVV+ M K KVLVKVHPEGK+VV            P+ RVALRNDSY LH ILP+KVDPLV+LM VEKVPDSTY+MIGGLD+QIKEIKEVIELP+KHPELFE+LGIAQP+
Sbjct:    6 PEQMELEEG--KAGSGLRQYYLSKIEELQLIVNDKSQNLRRLQAQRNELNAKVRLLREELQLLQEQGSYVGEVVRAMDKKKVLVKVHPEGKFVVDVDKNIDINDVTPNCRVALRNDSYTLHKILPNKVDPLVSLMMVEKVPDSTYEMIGGLDKQIKEIKEVIELPVKHPELFEALGIAQPK 184

HSP 2 Score: 31.9574 bits (71), Expect = 1.162e-67
Identity = 14/17 (82.35%), Postives = 14/17 (82.35%), Query Frame = 1 
Query:  661 KGVLLYGPPATGTTLWA 711
            KGVLLYGPP TG TL A
Sbjct:  184 KGVLLYGPPGTGKTLLA 200
[Close]
BLAST of EY724975 vs. ExPASy Swiss-Prot
Match: PRS8_MOUSE (26S protease regulatory subunit 8 OS=Mus musculus GN=Psmc5 PE=1 SV=1)

HSP 1 Score: 246.899 bits (629), Expect = 1.162e-67
Identity = 127/181 (70.17%), Postives = 146/181 (80.66%), Query Frame = 2 
Query:  122 PEEVCAAKGASKQGEGLRQYYLQHIHELQLQVRQKTHNLNRLEAQRNELNSRVRMLKEELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPR 664
            PE++   +G  K G GLRQYYL  I ELQL V  K+ NL RL+AQRNELN++VR+L+EELQLLQE GSYVGEVV+ M K KVLVKVHPEGK+VV            P+ RVALRNDSY LH ILP+KVDPLV+LM VEKVPDSTY+MIGGLD+QIKEIKEVIELP+KHPELFE+LGIAQP+
Sbjct:    6 PEQMELEEG--KAGSGLRQYYLSKIEELQLIVNDKSQNLRRLQAQRNELNAKVRLLREELQLLQEQGSYVGEVVRAMDKKKVLVKVHPEGKFVVDVDKNIDINDVTPNCRVALRNDSYTLHKILPNKVDPLVSLMMVEKVPDSTYEMIGGLDKQIKEIKEVIELPVKHPELFEALGIAQPK 184

HSP 2 Score: 31.9574 bits (71), Expect = 1.162e-67
Identity = 14/17 (82.35%), Postives = 14/17 (82.35%), Query Frame = 1 
Query:  661 KGVLLYGPPATGTTLWA 711
            KGVLLYGPP TG TL A
Sbjct:  184 KGVLLYGPPGTGKTLLA 200
[Close]
BLAST of EY724975 vs. ExPASy Swiss-Prot
Match: PRS8_HUMAN (26S protease regulatory subunit 8 OS=Homo sapiens GN=PSMC5 PE=1 SV=1)

HSP 1 Score: 246.899 bits (629), Expect = 1.162e-67
Identity = 127/181 (70.17%), Postives = 146/181 (80.66%), Query Frame = 2 
Query:  122 PEEVCAAKGASKQGEGLRQYYLQHIHELQLQVRQKTHNLNRLEAQRNELNSRVRMLKEELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPR 664
            PE++   +G  K G GLRQYYL  I ELQL V  K+ NL RL+AQRNELN++VR+L+EELQLLQE GSYVGEVV+ M K KVLVKVHPEGK+VV            P+ RVALRNDSY LH ILP+KVDPLV+LM VEKVPDSTY+MIGGLD+QIKEIKEVIELP+KHPELFE+LGIAQP+
Sbjct:    6 PEQMELEEG--KAGSGLRQYYLSKIEELQLIVNDKSQNLRRLQAQRNELNAKVRLLREELQLLQEQGSYVGEVVRAMDKKKVLVKVHPEGKFVVDVDKNIDINDVTPNCRVALRNDSYTLHKILPNKVDPLVSLMMVEKVPDSTYEMIGGLDKQIKEIKEVIELPVKHPELFEALGIAQPK 184

HSP 2 Score: 31.9574 bits (71), Expect = 1.162e-67
Identity = 14/17 (82.35%), Postives = 14/17 (82.35%), Query Frame = 1 
Query:  661 KGVLLYGPPATGTTLWA 711
            KGVLLYGPP TG TL A
Sbjct:  184 KGVLLYGPPGTGKTLLA 200
[Close]
BLAST of EY724975 vs. ExPASy Swiss-Prot
Match: PRS8_BOVIN (26S protease regulatory subunit 8 OS=Bos taurus GN=PSMC5 PE=2 SV=1)

HSP 1 Score: 246.899 bits (629), Expect = 1.162e-67
Identity = 127/181 (70.17%), Postives = 146/181 (80.66%), Query Frame = 2 
Query:  122 PEEVCAAKGASKQGEGLRQYYLQHIHELQLQVRQKTHNLNRLEAQRNELNSRVRMLKEELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPR 664
            PE++   +G  K G GLRQYYL  I ELQL V  K+ NL RL+AQRNELN++VR+L+EELQLLQE GSYVGEVV+ M K KVLVKVHPEGK+VV            P+ RVALRNDSY LH ILP+KVDPLV+LM VEKVPDSTY+MIGGLD+QIKEIKEVIELP+KHPELFE+LGIAQP+
Sbjct:    6 PEQMELEEG--KAGSGLRQYYLSKIEELQLIVNDKSQNLRRLQAQRNELNAKVRLLREELQLLQEQGSYVGEVVRAMDKKKVLVKVHPEGKFVVDVDKNIDINDVTPNCRVALRNDSYTLHKILPNKVDPLVSLMMVEKVPDSTYEMIGGLDKQIKEIKEVIELPVKHPELFEALGIAQPK 184

HSP 2 Score: 31.9574 bits (71), Expect = 1.162e-67
Identity = 14/17 (82.35%), Postives = 14/17 (82.35%), Query Frame = 1 
Query:  661 KGVLLYGPPATGTTLWA 711
            KGVLLYGPP TG TL A
Sbjct:  184 KGVLLYGPPGTGKTLLA 200
[Close]
BLAST of EY724975 vs. ExPASy Swiss-Prot
Match: PRS8_DROME (26S protease regulatory subunit 8 OS=Drosophila melanogaster GN=Pros45 PE=1 SV=2)

HSP 1 Score: 243.047 bits (619), Expect = 1.652e-66
Identity = 122/174 (70.11%), Postives = 142/174 (81.61%), Query Frame = 2 
Query:  143 KGASKQGEGLRQYYLQHIHELQLQVRQKTHNLNRLEAQRNELNSRVRMLKEELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPR 664
            + A  +GEG R YY+Q I ELQL V +K  NL RL+AQRNELN++VRML+EELQLLQE GSYVGEVVK M K KVLVKVHPEGK+VV            P+ RVALRN+SY LH ILP+KVDPLV+LM VEKVPDSTY+M+GGLD+QIKEIKEVIELP+KHPELF++LGIAQP+
Sbjct:   10 ESAYHKGEGFRSYYIQKIEELQLVVAEKHQNLRRLQAQRNELNAKVRMLREELQLLQEQGSYVGEVVKPMDKKKVLVKVHPEGKFVVDLDKNIDINDVTPNCRVALRNESYTLHKILPNKVDPLVSLMMVEKVPDSTYEMVGGLDKQIKEIKEVIELPVKHPELFDALGIAQPK 183

HSP 2 Score: 31.9574 bits (71), Expect = 1.652e-66
Identity = 14/17 (82.35%), Postives = 14/17 (82.35%), Query Frame = 1 
Query:  661 KGVLLYGPPATGTTLWA 711
            KGVLLYGPP TG TL A
Sbjct:  183 KGVLLYGPPGTGKTLLA 199
[Close]
BLAST of EY724975 vs. ExPASy Swiss-Prot
Match: PRS8_XENLA (26S protease regulatory subunit 8 OS=Xenopus laevis GN=psmc5 PE=2 SV=2)

HSP 1 Score: 241.891 bits (616), Expect = 3.660e-66
Identity = 122/171 (71.35%), Postives = 140/171 (81.87%), Query Frame = 2 
Query:  152 SKQGEGLRQYYLQHIHELQLQVRQKTHNLNRLEAQRNELNSRVRMLKEELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPR 664
            S+ G GLRQYYL  I +LQL V  K+ NL RL+AQRNELN++VR+L+EELQLLQE GSYVGEVV+ M K KVLVKVHPEGK+VV            P+ RVALRNDSY LH ILP+KVDPLV+LM VEKVPDSTY+MIGGLD+QIKEIKEVIELP+KHPE FE+LGIAQP+
Sbjct:    9 SRGGTGLRQYYLSKIEDLQLVVNDKSQNLRRLQAQRNELNAKVRLLREELQLLQEQGSYVGEVVRAMDKKKVLVKVHPEGKFVVDVDKNIDINDVTPNCRVALRNDSYTLHKILPNKVDPLVSLMMVEKVPDSTYEMIGGLDKQIKEIKEVIELPVKHPEHFEALGIAQPK 179

HSP 2 Score: 31.9574 bits (71), Expect = 3.660e-66
Identity = 14/17 (82.35%), Postives = 14/17 (82.35%), Query Frame = 1 
Query:  661 KGVLLYGPPATGTTLWA 711
            KGVLLYGPP TG TL A
Sbjct:  179 KGVLLYGPPGTGKTLLA 195
[Close]
BLAST of EY724975 vs. ExPASy Swiss-Prot
Match: PRS8_DICDI (26S protease regulatory subunit 8 OS=Dictyostelium discoideum GN=psmC5 PE=1 SV=2)

HSP 1 Score: 239.195 bits (609), Expect = 2.349e-65
Identity = 117/167 (70.06%), Postives = 140/167 (83.83%), Query Frame = 2 
Query:  164 EGLRQYYLQHIHELQLQVRQKTHNLNRLEAQRNELNSRVRMLKEELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPR 664
            +G++ YY   I EL+++V +K  +L RLEAQRNELN+RVRMLKEELQLL  PGS+V EVVK+MGKNKVLVKV+PEGK+VV            PSTR AL+++SY LH ILP+K+DPLV+LMKVEK+PDSTYDM+GGLD+QIKEIKEVIELPIKHPELFESLGIAQP+
Sbjct:   14 KGIKSYYCSKIEELEIKVNEKAQDLRRLEAQRNELNNRVRMLKEELQLLTNPGSHVAEVVKLMGKNKVLVKVNPEGKFVVDIDPTVDIAKLTPSTRAALKHESYTLHRILPNKIDPLVSLMKVEKIPDSTYDMVGGLDKQIKEIKEVIELPIKHPELFESLGIAQPK 180

HSP 2 Score: 31.9574 bits (71), Expect = 2.349e-65
Identity = 14/17 (82.35%), Postives = 14/17 (82.35%), Query Frame = 1 
Query:  661 KGVLLYGPPATGTTLWA 711
            KGVLLYGPP TG TL A
Sbjct:  180 KGVLLYGPPGTGKTLLA 196
The following BLAST results are available for this feature:
BLAST of EY724975 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 119
Match NameE-valueIdentityDescription
PRS8A_ARATH8.185e-9083.7626S protease regulatory subunit 8 homolog A OS=Ara... [more]
PRS8B_ARATH3.391e-8580.7126S protease regulatory subunit 8 homolog B OS=Ara... [more]
PRS8_RAT1.162e-6770.1726S protease regulatory subunit 8 OS=Rattus norveg... [more]
PRS8_PIG1.162e-6770.1726S protease regulatory subunit 8 OS=Sus scrofa GN... [more]
PRS8_MOUSE1.162e-6770.1726S protease regulatory subunit 8 OS=Mus musculus ... [more]
PRS8_HUMAN1.162e-6770.1726S protease regulatory subunit 8 OS=Homo sapiens ... [more]
PRS8_BOVIN1.162e-6770.1726S protease regulatory subunit 8 OS=Bos taurus GN... [more]
PRS8_DROME1.652e-6670.1126S protease regulatory subunit 8 OS=Drosophila me... [more]
PRS8_XENLA3.660e-6671.3526S protease regulatory subunit 8 OS=Xenopus laevi... [more]
PRS8_DICDI2.349e-6570.0626S protease regulatory subunit 8 OS=Dictyostelium... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-703-084-B07-CT.F Sweet orange fruit, development stadium (4 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY724975 ID=EY724975; Name=EY724975; organism=Citrus sinensis; type=EST; length=869bp
CACGCGTCCGCCCGAACGAGACTTGATCCGAAAGACAATTAGATTCGAAC
AAATCGAACTCGTTCACTCGGAAAGAATGGCCGCTGTAGGAATCGAAACG
AAGCAGCCGGAATCGGCGGCGCCAGAGGAGGTGTGTGCGGCGAAAGGAGC
ATCGAAACAAGGAGAGGGATTGAGGCAATACTACCTCCAACACATTCACG
AACTCCAGCTCCAAGTCCGTCAAAAAACCCACAATCTCAATCGCCTCGAA
GCCCAGCGTAACGAACTCAATTCTAGAGTGCGGATGCTTAAAGAAGAATT
ACAGCTTCTTCAAGAGCCTGGATCCTATGTCGGTGAAGTTGTTAAAGTTA
TGGGAAAAAATAAGGTTCTTGTTAAGGTTCATCCTGAGGGGAAATATGTT
GTTGATATTGATAAAAGTATTGATATAACCAAGATCACTCCGTCAACAAG
AGTTGCACTTCGTAATGACAGCTATGTTCTTCATTTGATCCTGCCAAGCA
AAGTAGATCCCCTGGTCAACCTTATGAAGGTTGAAAAGGTCCCTGATTCT
ACATATGACATGATTGGTGGCCTTGATCAGCAAATTAAGGAGATCAAGGA
GGTCATTGAGCTTCCTATCAAACATCCCGAATTGTTCGAGTCTCTTGGAA
TAGCTCAACCAAGGGTGTATTGCTATATGGACCACCTGCTACCGGCACAA
CACTGTGGGCAAGCCGGCCAGTGTACGCGCAACACGAGCAGATGCGTGGG
CCGGTGGNNNGNGGNNNNNNNNNNNGNAACTNCNGGCNNGCGNNGCGGGG
GGGNGNGCGCCGCACANGGGGNGCGNCGGGGGCCGGTCGTCCCGCGCCGC
CCACGGGCCGCCGCGCGCG
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