EY721995

Overview
NameEY721995
Unique NameEY721995
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length822
Libraries
Library NameType
Sweet orange fruit, development stadium (4 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY721995 vs. ExPASy Swiss-Prot
Match: RCA1_LARTR (Ribulose bisphosphate carboxylase/oxygenase activase 1, chloroplastic OS=Larrea tridentata GN=RCA1 PE=2 SV=1)

HSP 1 Score: 219.164 bits (557), Expect = 2.694e-73
Identity = 106/136 (77.94%), Postives = 115/136 (84.56%), Query Frame = 1 
Query:   58 EIYWAPTREDRIGVCKGIFRNDNVADDDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWXXXXXXXXXXKSLVNSKEAAPTFEQPRMTMEKLLEYGNMIVQEQENVKRVQLADKYLSEAALGEANEDAIQSGNFYG 465
            + YWAPTREDRIGVCKGIFR DNVADDDIVKLVDTFPGQSIDFFGALRARVY DEVRKW          K LVNSKE  P+FEQP+MT++KLL YG M+VQEQENVKRVQLADKY+SEAALG+AN DAI+ G FYG
Sbjct:  302 KFYWAPTREDRIGVCKGIFRTDNVADDDIVKLVDTFPGQSIDFFGALRARVYHDEVRKWVSEVGVDTIGKKLVNSKEGPPSFEQPKMTIDKLLGYGGMLVQEQENVKRVQLADKYMSEAALGDANNDAIKRGTFYG 437

HSP 2 Score: 60.8474 bits (146), Expect = 2.694e-73
Identity = 28/34 (82.35%), Postives = 31/34 (91.18%), Query Frame = 2 
Query:  500 GGKAAQQM-NVPVPEGCTDPTAENFDPTARSDDG 598
            GG+AAQQ+ NVPVPEGCTDP A N+DPTARSDDG
Sbjct:  437 GGQAAQQVGNVPVPEGCTDPQATNYDPTARSDDG 470

HSP 3 Score: 38.891 bits (89), Expect = 2.694e-73
Identity = 17/18 (94.44%), Postives = 18/18 (100.00%), Query Frame = 2 
Query:    8 SDFSTLYAPLIRDGRMEK 61
            +DFSTLYAPLIRDGRMEK
Sbjct:  285 NDFSTLYAPLIRDGRMEK 302
[Close]
BLAST of EY721995 vs. ExPASy Swiss-Prot
Match: RCA_ORYSJ (Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Oryza sativa subsp. japonica GN=RCA PE=1 SV=2)

HSP 1 Score: 210.69 bits (535), Expect = 9.710e-70
Identity = 98/136 (72.06%), Postives = 117/136 (86.03%), Query Frame = 1 
Query:   58 EIYWAPTREDRIGVCKGIFRNDNVADDDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWXXXXXXXXXXKSLVNSKEAAPTFEQPRMTMEKLLEYGNMIVQEQENVKRVQLADKYLSEAALGEANEDAIQSGNFYG 465
            + YWAPTR+DR+GVCKGIFR DNV D+DIVK+VD+FPGQSIDFFGALRARVYDDEVRKW          K LVNS+E  P FEQP+MT+EKL+EYG M+V+EQENVKRVQLA++YLSEAALG+AN DA+++G+FYG
Sbjct:  293 KFYWAPTRDDRVGVCKGIFRTDNVPDEDIVKIVDSFPGQSIDFFGALRARVYDDEVRKWVSDTGVENIGKRLVNSREGPPEFEQPKMTIEKLMEYGYMLVKEQENVKRVQLAEQYLSEAALGDANSDAMKTGSFYG 428

HSP 2 Score: 57.3806 bits (137), Expect = 9.710e-70
Identity = 26/33 (78.79%), Postives = 29/33 (87.88%), Query Frame = 2 
Query:  503 GKAAQQM-NVPVPEGCTDPTAENFDPTARSDDG 598
            G+ AQQ  N+PVPEGCTDP A+NFDPTARSDDG
Sbjct:  428 GQGAQQAGNLPVPEGCTDPVAKNFDPTARSDDG 460

HSP 3 Score: 38.891 bits (89), Expect = 9.710e-70
Identity = 17/18 (94.44%), Postives = 18/18 (100.00%), Query Frame = 2 
Query:    8 SDFSTLYAPLIRDGRMEK 61
            +DFSTLYAPLIRDGRMEK
Sbjct:  276 NDFSTLYAPLIRDGRMEK 293
[Close]
BLAST of EY721995 vs. ExPASy Swiss-Prot
Match: RCA_ARATH (Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Arabidopsis thaliana GN=RCA PE=1 SV=2)

HSP 1 Score: 201.445 bits (511), Expect = 1.646e-69
Identity = 98/136 (72.06%), Postives = 108/136 (79.41%), Query Frame = 1 
Query:   58 EIYWAPTREDRIGVCKGIFRNDNVADDDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWXXXXXXXXXXKSLVNSKEAAPTFEQPRMTMEKLLEYGNMIVQEQENVKRVQLADKYLSEAALGEANEDAIQSGNFYG 465
            + YWAPTREDRIGVCKGIFR D + D+DIV LVD FPGQSIDFFGALRARVYDDEVRK+          K LVNS+E  P FEQP MT EKL+EYGNM+V EQENVKRVQLA+ YLS+AALG+AN DAI  G FYG
Sbjct:  302 KFYWAPTREDRIGVCKGIFRTDKIKDEDIVTLVDQFPGQSIDFFGALRARVYDDEVRKFVESLGVEKIGKRLVNSREGPPVFEQPEMTYEKLMEYGNMLVMEQENVKRVQLAETYLSQAALGDANADAIGRGTFYG 437

HSP 2 Score: 65.855 bits (159), Expect = 1.646e-69
Identity = 28/32 (87.50%), Postives = 30/32 (93.75%), Query Frame = 2 
Query:  503 GKAAQQMNVPVPEGCTDPTAENFDPTARSDDG 598
            GK AQQ+N+PVPEGCTDP AENFDPTARSDDG
Sbjct:  437 GKGAQQVNLPVPEGCTDPVAENFDPTARSDDG 468

HSP 3 Score: 38.891 bits (89), Expect = 1.646e-69
Identity = 17/18 (94.44%), Postives = 18/18 (100.00%), Query Frame = 2 
Query:    8 SDFSTLYAPLIRDGRMEK 61
            +DFSTLYAPLIRDGRMEK
Sbjct:  285 NDFSTLYAPLIRDGRMEK 302
[Close]
BLAST of EY721995 vs. ExPASy Swiss-Prot
Match: RCAA_HORVU (Ribulose bisphosphate carboxylase/oxygenase activase A, chloroplastic OS=Hordeum vulgare GN=RCAA PE=2 SV=1)

HSP 1 Score: 208.764 bits (530), Expect = 8.032e-69
Identity = 96/136 (70.59%), Postives = 118/136 (86.76%), Query Frame = 1 
Query:   58 EIYWAPTREDRIGVCKGIFRNDNVADDDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWXXXXXXXXXXKSLVNSKEAAPTFEQPRMTMEKLLEYGNMIVQEQENVKRVQLADKYLSEAALGEANEDAIQSGNFYG 465
            + YWAPTR+DRIGVCKGIF+ DNV+D+ +VK+VDTFPGQSIDFFGALRARVYDDEVRKW          K LVNS++   TFEQP+MT+EKLLEYG+M+VQEQ+NVKRVQLAD Y+S+AALG+AN+DA+++G+FYG
Sbjct:  292 KFYWAPTRDDRIGVCKGIFQTDNVSDESVVKIVDTFPGQSIDFFGALRARVYDDEVRKWVGSTGIENIGKRLVNSRDGPVTFEQPKMTVEKLLEYGHMLVQEQDNVKRVQLADTYMSQAALGDANQDAMKTGSFYG 427

HSP 2 Score: 56.225 bits (134), Expect = 8.032e-69
Identity = 24/32 (75.00%), Postives = 27/32 (84.38%), Query Frame = 2 
Query:  503 GKAAQQMNVPVPEGCTDPTAENFDPTARSDDG 598
            GK AQQ  +PVPEGCTD  A+N+DPTARSDDG
Sbjct:  427 GKGAQQGTLPVPEGCTDQNAKNYDPTARSDDG 458

HSP 3 Score: 38.891 bits (89), Expect = 8.032e-69
Identity = 17/18 (94.44%), Postives = 18/18 (100.00%), Query Frame = 2 
Query:    8 SDFSTLYAPLIRDGRMEK 61
            +DFSTLYAPLIRDGRMEK
Sbjct:  275 NDFSTLYAPLIRDGRMEK 292
[Close]
BLAST of EY721995 vs. ExPASy Swiss-Prot
Match: RCA_SPIOL (Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Spinacia oleracea PE=1 SV=2)

HSP 1 Score: 199.134 bits (505), Expect = 1.578e-66
Identity = 94/136 (69.12%), Postives = 108/136 (79.41%), Query Frame = 1 
Query:   58 EIYWAPTREDRIGVCKGIFRNDNVADDDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWXXXXXXXXXXKSLVNSKEAAPTFEQPRMTMEKLLEYGNMIVQEQENVKRVQLADKYLSEAALGEANEDAIQSGNFYG 465
            + YWAPTREDRIGVC GIF+ D V  + +VKLVD FPGQSIDFFGALRARVY DEVRKW          K LVNSK+  P FEQP MT++KL+EYGNM+VQEQENVKRVQLAD+Y+S AALG+AN+DAI  G F+G
Sbjct:  300 KFYWAPTREDRIGVCTGIFKTDKVPAEHVVKLVDAFPGQSIDFFGALRARVYHDEVRKWVNSVGVDNVGKKLVNSKDGPPVFEQPEMTLQKLMEYGNMLVQEQENVKRVQLADQYMSSAALGDANKDAIDRGTFFG 435

HSP 2 Score: 58.151 bits (139), Expect = 1.578e-66
Identity = 24/32 (75.00%), Postives = 30/32 (93.75%), Query Frame = 2 
Query:  503 GKAAQQMNVPVPEGCTDPTAENFDPTARSDDG 598
            GKAAQQ+++PV +GCTDP A+N+DPTARSDDG
Sbjct:  435 GKAAQQVSLPVAQGCTDPEAKNYDPTARSDDG 466

HSP 3 Score: 38.891 bits (89), Expect = 1.578e-66
Identity = 17/18 (94.44%), Postives = 18/18 (100.00%), Query Frame = 2 
Query:    8 SDFSTLYAPLIRDGRMEK 61
            +DFSTLYAPLIRDGRMEK
Sbjct:  283 NDFSTLYAPLIRDGRMEK 300
[Close]
BLAST of EY721995 vs. ExPASy Swiss-Prot
Match: RCA_MALDO (Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Malus domestica GN=RCA PE=2 SV=1)

HSP 1 Score: 222.631 bits (566), Expect = 1.756e-62
Identity = 108/136 (79.41%), Postives = 117/136 (86.03%), Query Frame = 1 
Query:   58 EIYWAPTREDRIGVCKGIFRNDNVADDDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWXXXXXXXXXXKSLVNSKEAAPTFEQPRMTMEKLLEYGNMIVQEQENVKRVQLADKYLSEAALGEANEDAIQSGNFYG 465
            + YWAPTREDRIGVC GIFR+DNVA +DIVKLVDTFPGQSIDFFGALRARVYDDEVRKW          K LVNSKE  PTFEQP+MT+EKLLEYGNM+VQEQENVKRVQLADKYLSEAALG+AN DA+ +G FYG
Sbjct:  302 KFYWAPTREDRIGVCIGIFRSDNVAKEDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWITGVGVDSIGKKLVNSKEGPPTFEQPKMTIEKLLEYGNMLVQEQENVKRVQLADKYLSEAALGDANSDAMNTGTFYG 437

HSP 2 Score: 38.891 bits (89), Expect = 1.756e-62
Identity = 17/18 (94.44%), Postives = 18/18 (100.00%), Query Frame = 2 
Query:    8 SDFSTLYAPLIRDGRMEK 61
            +DFSTLYAPLIRDGRMEK
Sbjct:  285 NDFSTLYAPLIRDGRMEK 302
[Close]
BLAST of EY721995 vs. ExPASy Swiss-Prot
Match: RCAB_HORVU (Ribulose bisphosphate carboxylase/oxygenase activase B, chloroplastic OS=Hordeum vulgare GN=RCAB PE=2 SV=1)

HSP 1 Score: 216.468 bits (550), Expect = 1.226e-60
Identity = 102/136 (75.00%), Postives = 118/136 (86.76%), Query Frame = 1 
Query:   58 EIYWAPTREDRIGVCKGIFRNDNVADDDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWXXXXXXXXXXKSLVNSKEAAPTFEQPRMTMEKLLEYGNMIVQEQENVKRVQLADKYLSEAALGEANEDAIQSGNFYG 465
            + YWAPTREDRIGVCKGIFR DNV D+ +V+LVDTFPGQSIDFFGALRARVYDDEVRKW          K LVNS+E  PTF+QP+MT+EKL+EYG+M+VQEQENVKRVQLADKYLSEAALG+AN+DA+++G FYG
Sbjct:  289 KFYWAPTREDRIGVCKGIFRTDNVPDEAVVRLVDTFPGQSIDFFGALRARVYDDEVRKWVGEIGVENISKRLVNSREGPPTFDQPKMTIEKLMEYGHMLVQEQENVKRVQLADKYLSEAALGQANDDAMKTGAFYG 424

HSP 2 Score: 38.891 bits (89), Expect = 1.226e-60
Identity = 17/18 (94.44%), Postives = 18/18 (100.00%), Query Frame = 2 
Query:    8 SDFSTLYAPLIRDGRMEK 61
            +DFSTLYAPLIRDGRMEK
Sbjct:  272 NDFSTLYAPLIRDGRMEK 289
[Close]
BLAST of EY721995 vs. ExPASy Swiss-Prot
Match: RCA_PHAAU (Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Phaseolus aureus GN=RCA PE=2 SV=2)

HSP 1 Score: 213.772 bits (543), Expect = 7.850e-60
Identity = 100/135 (74.07%), Postives = 116/135 (85.93%), Query Frame = 1 
Query:   58 EIYWAPTREDRIGVCKGIFRNDNVADDDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWXXXXXXXXXXKSLVNSKEAAPTFEQPRMTMEKLLEYGNMIVQEQENVKRVQLADKYLSEAALGEANEDAIQSGNFY 462
            + YWAPTR+DR+GVCKGIFR D V ++DI KLVDTFPGQSIDFFGALRARVYDDEVRKW          K LVNSKE  PTF+QP+M+++KLL+YGNM+VQEQENVKRVQLADKYL+EAALG ANEDAI+SG+F+
Sbjct:  304 KFYWAPTRDDRVGVCKGIFRTDGVPEEDITKLVDTFPGQSIDFFGALRARVYDDEVRKWISGVGVDATGKKLVNSKEGPPTFDQPKMSLDKLLQYGNMLVQEQENVKRVQLADKYLNEAALGNANEDAIKSGSFF 438

HSP 2 Score: 38.891 bits (89), Expect = 7.850e-60
Identity = 17/18 (94.44%), Postives = 18/18 (100.00%), Query Frame = 2 
Query:    8 SDFSTLYAPLIRDGRMEK 61
            +DFSTLYAPLIRDGRMEK
Sbjct:  287 NDFSTLYAPLIRDGRMEK 304
[Close]
BLAST of EY721995 vs. ExPASy Swiss-Prot
Match: RCA2_LARTR (Ribulose bisphosphate carboxylase/oxygenase activase 2, chloroplastic OS=Larrea tridentata GN=RCA2 PE=2 SV=1)

HSP 1 Score: 213.386 bits (542), Expect = 1.024e-59
Identity = 101/134 (75.37%), Postives = 115/134 (85.82%), Query Frame = 1 
Query:   58 EIYWAPTREDRIGVCKGIFRNDNVADDDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWXXXXXXXXXXKSLVNSKEAAPTFEQPRMTMEKLLEYGNMIVQEQENVKRVQLADKYLSEAALGEANEDAIQSGNF 459
            + YWAPTREDRIGVCKGIFR DNV ++DIVK+VD FPGQSIDFFGALRARVYDDEVRKW          K LVNSKE  PTFEQP+MT++KLL+YGNM+V+EQENVKRVQLADKY+SEAALG+AN+DAI+ G F
Sbjct:  302 KFYWAPTREDRIGVCKGIFRTDNVPEEDIVKVVDQFPGQSIDFFGALRARVYDDEVRKWVSEVGVDTIGKKLVNSKEGPPTFEQPKMTIDKLLQYGNMLVEEQENVKRVQLADKYMSEAALGDANQDAIKRGTF 435

HSP 2 Score: 38.891 bits (89), Expect = 1.024e-59
Identity = 17/18 (94.44%), Postives = 18/18 (100.00%), Query Frame = 2 
Query:    8 SDFSTLYAPLIRDGRMEK 61
            +DFSTLYAPLIRDGRMEK
Sbjct:  285 NDFSTLYAPLIRDGRMEK 302
[Close]
BLAST of EY721995 vs. ExPASy Swiss-Prot
Match: RCA_MAIZE (Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Zea mays GN=RCA1 PE=2 SV=3)

HSP 1 Score: 207.994 bits (528), Expect = 4.198e-58
Identity = 98/135 (72.59%), Postives = 116/135 (85.93%), Query Frame = 1 
Query:   58 EIYWAPTREDRIGVCKGIFRNDNVADDDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWXXXXXXXXXXKSLVNSKEAAPTFEQPRMTMEKLLEYGNMIVQEQENVKRVQLADKYLSEAALGEANEDAIQSGNFY 462
            + YWAPTREDRIGVCKGIFR D V ++ +V+LVDTFPGQSIDFFGALRARVYDDEVR+W          + LVNSKE  PTFEQP++T+EKLLEYG+M+V EQENVKRVQLADKYL+EAALGEANEDA+++G+F+
Sbjct:  298 KFYWAPTREDRIGVCKGIFRTDGVDEEHVVQLVDTFPGQSIDFFGALRARVYDDEVRRWVSETGVENIARKLVNSKEGPPTFEQPKITIEKLLEYGHMLVAEQENVKRVQLADKYLNEAALGEANEDAMKTGSFF 432

HSP 2 Score: 38.891 bits (89), Expect = 4.198e-58
Identity = 17/18 (94.44%), Postives = 18/18 (100.00%), Query Frame = 2 
Query:    8 SDFSTLYAPLIRDGRMEK 61
            +DFSTLYAPLIRDGRMEK
Sbjct:  281 NDFSTLYAPLIRDGRMEK 298
The following BLAST results are available for this feature:
BLAST of EY721995 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 18
Match NameE-valueIdentityDescription
RCA1_LARTR2.694e-7377.94Ribulose bisphosphate carboxylase/oxygenase activa... [more]
RCA_ORYSJ9.710e-7072.06Ribulose bisphosphate carboxylase/oxygenase activa... [more]
RCA_ARATH1.646e-6972.06Ribulose bisphosphate carboxylase/oxygenase activa... [more]
RCAA_HORVU8.032e-6970.59Ribulose bisphosphate carboxylase/oxygenase activa... [more]
RCA_SPIOL1.578e-6669.12Ribulose bisphosphate carboxylase/oxygenase activa... [more]
RCA_MALDO1.756e-6279.41Ribulose bisphosphate carboxylase/oxygenase activa... [more]
RCAB_HORVU1.226e-6075.00Ribulose bisphosphate carboxylase/oxygenase activa... [more]
RCA_PHAAU7.850e-6074.07Ribulose bisphosphate carboxylase/oxygenase activa... [more]
RCA2_LARTR1.024e-5975.37Ribulose bisphosphate carboxylase/oxygenase activa... [more]
RCA_MAIZE4.198e-5872.59Ribulose bisphosphate carboxylase/oxygenase activa... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-703-030-F02-CT.F Sweet orange fruit, development stadium (4 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY721995 ID=EY721995; Name=EY721995; organism=Citrus sinensis; type=EST; length=822bp
CCACGCGTCCGACTTCTCAACATTGTATGCTCCTCTCATCCGTGATGGTC
GTATGGAGAAATCTACTGGGCACCTACAAGGGAGGACCGTATTGGTGTGT
GCAAGGGTATCTTCAGGAATGACAATGTGGCTGATGACGACATTGTCAAG
CTTGTCGACACCTTCCCCGGCCAATCCATTGATTTCTTTGGAGCCCTGAG
GGCCAGAGTCTATGATGATGAGGTGAGGAAGTGGATCTCTGGTGTTGGTG
TTGGAAGCATTGGCAAGAGCCTTGTGAACTCAAAGGAAGCAGCCCCAACA
TTCGAGCAACCAAGGATGACAATGGAGAAGCTGCTCGAGTACGGAAACAT
GATTGTGCAAGAGCAAGAGAATGTGAAGAGAGTTCAGTTGGCTGACAAGT
ATTTGAGTGAGGCCGCTCTTGGTGAAGCTAATGAAGATGCTATTCAGAGT
GGAAATTTCTATGGTTAGCCCCCTTGATCCTAGCTAAGAGGCCTTGATGG
GAGGCAAAGCAGCCCAGCAAATGAATGTTCCAGTTCCTGAAGGATGTACT
GATCCTACTGCAGAAAACTTTGATCCAACTGCAAGAAGTGATGATGGGAA
GCTGCCAATACACGCTTTAGGCCCTGTTTCTAAGGGTTGAAAATGTCCCA
CCTTGGCTTAGATGTGAGATTTTCAATAAGATGGTTGCATTTCCAGTTCC
TTTTTAATCCAAAAGAACCCCCAATTTTGGTTTTAATGACAAGTCTGATT
CGAATTTTTAAAAAAGAAAAAAACAGGGGGCCGCTCTAAGAGGACCAACG
TTTCGCTACCCGTCCGTGCCAC
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