EY720867 | Citrus Genome Database

Overview


Name	EY720867
Unique Name	EY720867
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	869

Libraries


Library Name	Type
Sweet orange fruit, development stadium (4 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY720867 vs. ExPASy Swiss-Prot
Match: IF4A3_NICPL (Eukaryotic initiation factor 4A-3 OS=Nicotiana plumbaginifolia PE=2 SV=1)

HSP 1 Score: 197.978 bits (502), Expect = 4.955e-58
Identity = 98/124 (79.03%), Postives = 110/124 (88.71%), Query Frame = 2

Query:  125 KMVFETTEGVEAITSFDAMGIKDDLLRGIYQYGFEKPSAIQQRAVMPIIKGRDVIAQAQSGTGKTSMIALTVCQTVDTSTXEVQALILSPTKELATQTEKVILGNGDFINIHPHACVGGQSVRE 496
            ++VFET++GVE I SF  MGIKDDLLRG+YQYGFEKPSAIQQRAV+PII GRDVIAQAQSGTGKTSMIALTVCQ VDT + EVQALILSPT+ELA QTEKVIL  GD+IN+  HAC+GG+SV E
Sbjct:    5 RLVFETSKGVEPIASFAEMGIKDDLLRGVYQYGFEKPSAIQQRAVLPIISGRDVIAQAQSGTGKTSMIALTVCQIVDTKSSEVQALILSPTRELAAQTEKVILAIGDYINVQAHACIGGKSVGE 128

HSP 2 Score: 48.521 bits (114), Expect = 4.955e-58
Identity = 32/86 (37.21%), Postives = 46/86 (53.49%), Query Frame = 3

Query:  483 KVSGKDMTKVKHGIHVVSGTPGKGRDLITRKPCRTTTS*FLVADNLLKMLPPGSTDRCAYAVRSLRAGPP--AGVALRRPLPPEVM 734
            K  G+D+ K++HG+ VVSGTPG+  D+I R+  RT     L+ D   +ML  G  D+     R L   PP    V +   LP E++
Sbjct:  124 KSVGEDIRKLEHGVQVVSGTPGRVCDMIKRRTLRTRGIKLLILDESDEMLSRGFKDQIYDVYRYL---PPELQVVLISATLPNEIL 206

[Close]

BLAST of EY720867 vs. ExPASy Swiss-Prot
Match: RH2_ORYSJ (DEAD-box ATP-dependent RNA helicase 2 OS=Oryza sativa subsp. japonica GN=Os01g0639100 PE=2 SV=1)

HSP 1 Score: 191.43 bits (485), Expect = 9.162e-57
Identity = 94/123 (76.42%), Postives = 108/123 (87.80%), Query Frame = 2

Query:  128 MVFETTEGVEAITSFDAMGIKDDLLRGIYQYGFEKPSAIQQRAVMPIIKGRDVIAQAQSGTGKTSMIALTVCQTVDTSTXEVQALILSPTKELATQTEKVILGNGDFINIHPHACVGGQSVRE 496
            + FET+ GVE I+SFD MGI+DDLLRGIY YGFEKPSAIQQRAV+PII GRDVIAQAQSGTGKTSMI+L+VCQ VDT+  EVQALILSPT+ELA QTE+V+L  GDFINI  HAC+GG+S+ E
Sbjct:   19 LTFETSPGVEVISSFDQMGIRDDLLRGIYAYGFEKPSAIQQRAVLPIISGRDVIAQAQSGTGKTSMISLSVCQIVDTAVREVQALILSPTRELAAQTERVMLAIGDFINIQVHACIGGKSIGE 141

HSP 2 Score: 50.8322 bits (120), Expect = 9.162e-57
Identity = 25/57 (43.86%), Postives = 36/57 (63.16%), Query Frame = 3

Query:  483 KVSGKDMTKVKHGIHVVSGTPGKGRDLITRKPCRTTTS*FLVADNLLKMLPPGSTDR 653
            K  G+D+ K++HG+HVVSGTPG+  D+I R+  RT     L+ D   +ML  G  D+
Sbjct:  137 KSIGEDIRKLEHGVHVVSGTPGRVCDMIKRRTLRTRAIKLLILDEADEMLGRGFKDQ 193

[Close]

BLAST of EY720867 vs. ExPASy Swiss-Prot
Match: RH2_ARATH (DEAD-box ATP-dependent RNA helicase 2 OS=Arabidopsis thaliana GN=RH2 PE=2 SV=2)

HSP 1 Score: 191.43 bits (485), Expect = 1.194e-56
Identity = 95/124 (76.61%), Postives = 109/124 (87.90%), Query Frame = 2

Query:  125 KMVFETTEGVEAITSFDAMGIKDDLLRGIYQYGFEKPSAIQQRAVMPIIKGRDVIAQAQSGTGKTSMIALTVCQTVDTSTXEVQALILSPTKELATQTEKVILGNGDFINIHPHACVGGQSVRE 496
            K+VFETT+G+E ITSF+ MGIK+D+LRG+Y+YGFEKPSAIQQRAVMPI++GRDVIAQAQSGTGKTSMIAL+VCQ VDTS+ EVQALILSPT+ELATQTEK I   G   NI  HAC+GG SV E
Sbjct:   22 KLVFETTDGIEPITSFNDMGIKEDVLRGVYEYGFEKPSAIQQRAVMPILQGRDVIAQAQSGTGKTSMIALSVCQVVDTSSREVQALILSPTRELATQTEKTIQAIGLHANIQAHACIGGNSVGE 145

HSP 2 Score: 50.447 bits (119), Expect = 1.194e-56
Identity = 24/54 (44.44%), Postives = 35/54 (64.81%), Query Frame = 3

Query:  492 GKDMTKVKHGIHVVSGTPGKGRDLITRKPCRTTTS*FLVADNLLKMLPPGSTDR 653
            G+D+ K++HG+HVVSGTPG+  D+I R+  RT     L+ D   +ML  G  D+
Sbjct:  144 GEDIRKLEHGVHVVSGTPGRVCDMIKRRSLRTRAIKLLILDESDEMLSRGFKDQ 197

[Close]

BLAST of EY720867 vs. ExPASy Swiss-Prot
Match: RH34_ORYSJ (DEAD-box ATP-dependent RNA helicase 34 OS=Oryza sativa subsp. japonica GN=Os03g0566800 PE=2 SV=1)

HSP 1 Score: 188.734 bits (478), Expect = 5.864e-56
Identity = 92/123 (74.80%), Postives = 108/123 (87.80%), Query Frame = 2

Query:  128 MVFETTEGVEAITSFDAMGIKDDLLRGIYQYGFEKPSAIQQRAVMPIIKGRDVIAQAQSGTGKTSMIALTVCQTVDTSTXEVQALILSPTKELATQTEKVILGNGDFINIHPHACVGGQSVRE 496
            + FET+ GVE I+SFD MGI++DLLRGIY YGFEKPSAIQQRAV+PII GRDVIAQAQSGTGKTSMI+L+VCQ VDT+  EVQALILSPT+ELA QTE+V+L  GD+INI  HAC+GG+S+ E
Sbjct:   19 LTFETSPGVEVISSFDQMGIREDLLRGIYAYGFEKPSAIQQRAVLPIISGRDVIAQAQSGTGKTSMISLSVCQIVDTAVREVQALILSPTRELAAQTERVMLAIGDYINIQVHACIGGKSIGE 141

HSP 2 Score: 50.8322 bits (120), Expect = 5.864e-56
Identity = 25/57 (43.86%), Postives = 36/57 (63.16%), Query Frame = 3

Query:  483 KVSGKDMTKVKHGIHVVSGTPGKGRDLITRKPCRTTTS*FLVADNLLKMLPPGSTDR 653
            K  G+D+ K++HG+HVVSGTPG+  D+I R+  RT     L+ D   +ML  G  D+
Sbjct:  137 KSIGEDIRKLEHGVHVVSGTPGRVCDMIKRRTLRTRAIKLLILDEADEMLGRGFKDQ 193

[Close]

BLAST of EY720867 vs. ExPASy Swiss-Prot
Match: FAL1_USTMA (ATP-dependent RNA helicase FAL1 OS=Ustilago maydis GN=FAL1 PE=3 SV=1)

HSP 1 Score: 167.162 bits (422), Expect = 3.949e-48
Identity = 77/124 (62.10%), Postives = 103/124 (83.06%), Query Frame = 2

Query:  125 KMVFETTEGVEAITSFDAMGIKDDLLRGIYQYGFEKPSAIQQRAVMPIIKGRDVIAQAQSGTGKTSMIALTVCQTVDTSTXEVQALILSPTKELATQTEKVILGNGDFINIHPHACVGGQSVRE 496
            K+ FE++E V+  T+FDAMG+K+DLLRGIY Y FEKPSAIQQRA++PII+GRDVIAQAQSGTGKT+  ++++ Q++DT+  E QAL+LSPT+ELA Q + V+L  GD++N+  HAC+GG SV E
Sbjct:   11 KLAFESSEHVKVATTFDAMGLKEDLLRGIYAYNFEKPSAIQQRAILPIIRGRDVIAQAQSGTGKTATFSISMLQSIDTTLRETQALVLSPTRELAIQIQSVVLALGDYLNVQCHACIGGTSVGE 134

HSP 2 Score: 46.2098 bits (108), Expect = 3.949e-48
Identity = 30/83 (36.14%), Postives = 45/83 (54.22%), Query Frame = 3

Query:  492 GKDMTKVKHGIHVVSGTPGKGRDLITRKPCRTTTS*FLVADNLLKMLPPGSTDRCAYAVRSLRAGPPAG--VALRRPLPPEVM 734
            G+D+ K+ +G H+VSGTPG+  D+I R+  RT     L+ D   ++L  G  D+     R L   PP+   V L   LP +V+
Sbjct:  133 GEDIRKLDYGQHIVSGTPGRVYDMIRRRHLRTKNIKMLILDESDELLNMGFKDQIYDVYRYL---PPSTQVVLLSATLPQDVL 212

[Close]

BLAST of EY720867 vs. ExPASy Swiss-Prot
Match: RH34_ARATH (DEAD-box ATP-dependent RNA helicase 34 OS=Arabidopsis thaliana GN=RH34 PE=2 SV=2)

HSP 1 Score: 165.236 bits (417), Expect = 2.523e-47
Identity = 78/123 (63.41%), Postives = 102/123 (82.93%), Query Frame = 2

Query:  128 MVFETTEGVEAITSFDAMGIKDDLLRGIYQYGFEKPSAIQQRAVMPIIKGRDVIAQAQSGTGKTSMIALTVCQTVDTSTXEVQALILSPTKELATQTEKVILGNGDFINIHPHACVGGQSVRE 496
            +VFETT+G++ I SFD MG+ D +LRG+Y YG++KPS IQQRA++PI+KGRDVIAQAQSGTGKTSMIA++VCQ V+ S+ +VQ L+LSP++ELA+QTEK I   G   NI  HAC+GG+S+ E
Sbjct:   10 LVFETTKGIKPIKSFDDMGMNDKVLRGVYDYGYKKPSEIQQRALVPILKGRDVIAQAQSGTGKTSMIAISVCQIVNISSRKVQVLVLSPSRELASQTEKTIQAIGAHTNIQAHACIGGKSIGE 132

HSP 2 Score: 45.4394 bits (106), Expect = 2.523e-47
Identity = 25/65 (38.46%), Postives = 36/65 (55.38%), Query Frame = 3

Query:  483 KVSGKDMTKVKHGIHVVSGTPGKGRDLITRKPCRTTTS*FLVADNLLKMLPPGSTDRCAYAVRSL 677
            K  G+D+ K++ G+H VSGTPG+  D+I R   +T     LV D   +ML  G  D+     R+L
Sbjct:  128 KSIGEDIKKLERGVHAVSGTPGRVYDMIKRGSLQTKAVKLLVLDESDEMLSKGLKDQIYDVYRAL 192

[Close]

BLAST of EY720867 vs. ExPASy Swiss-Prot
Match: IF4A3_CHICK (Eukaryotic initiation factor 4A-III OS=Gallus gallus GN=EIF4A3 PE=2 SV=1)

HSP 1 Score: 155.221 bits (391), Expect = 8.557e-45
Identity = 74/124 (59.68%), Postives = 97/124 (78.23%), Query Frame = 2

Query:  125 KMVFETTEGVEAITSFDAMGIKDDLLRGIYQYGFEKPSAIQQRAVMPIIKGRDVIAQAQSGTGKTSMIALTVCQTVDTSTXEVQALILSPTKELATQTEKVILGNGDFINIHPHACVGGQSVRE 496
            K+ FET+E V+   +FD MG+++DLLRGIY YGFEKPSAIQQRA+  IIKGRDVIAQ+QSGTGKT+  +++V Q +D    E QALIL+PT+ELA Q +K +L  GD++N+  HAC+GG +V E
Sbjct:   26 KVEFETSEEVDVTPTFDTMGLREDLLRGIYAYGFEKPSAIQQRAIKQIIKGRDVIAQSQSGTGKTATFSISVLQCLDIQVRETQALILAPTRELAVQIQKGLLALGDYMNVQCHACIGGTNVGE 149

HSP 2 Score: 46.9802 bits (110), Expect = 8.557e-45
Identity = 31/83 (37.35%), Postives = 45/83 (54.22%), Query Frame = 3

Query:  492 GKDMTKVKHGIHVVSGTPGKGRDLITRKPCRTTTS*FLVADNLLKMLPPGSTDRCAYAVRSLRAGPPAG--VALRRPLPPEVM 734
            G+D+ K+ +G HVV+GTPG+  D+I R+  RT     LV D   +ML  G  ++     R L   PPA   V +   LP E++
Sbjct:  148 GEDIRKLDYGQHVVAGTPGRVFDMIRRRSLRTRAIKMLVLDEADEMLNKGFKEQIYDVYRYL---PPATQVVLISATLPHEIL 227

[Close]

BLAST of EY720867 vs. ExPASy Swiss-Prot
Match: IF4A3_TAEGU (Eukaryotic initiation factor 4A-III OS=Taeniopygia guttata GN=EIF4A3 PE=2 SV=1)

HSP 1 Score: 155.221 bits (391), Expect = 8.558e-45
Identity = 74/124 (59.68%), Postives = 97/124 (78.23%), Query Frame = 2

Query:  125 KMVFETTEGVEAITSFDAMGIKDDLLRGIYQYGFEKPSAIQQRAVMPIIKGRDVIAQAQSGTGKTSMIALTVCQTVDTSTXEVQALILSPTKELATQTEKVILGNGDFINIHPHACVGGQSVRE 496
            K+ FET+E V+   +FD MG+++DLLRGIY YGFEKPSAIQQRA+  IIKGRDVIAQ+QSGTGKT+  +++V Q +D    E QALIL+PT+ELA Q +K +L  GD++N+  HAC+GG +V E
Sbjct:   24 KVEFETSEEVDVTPTFDTMGLREDLLRGIYAYGFEKPSAIQQRAIKQIIKGRDVIAQSQSGTGKTATFSISVLQCLDIQVRETQALILAPTRELAVQIQKGLLALGDYMNVQCHACIGGTNVGE 147

HSP 2 Score: 46.9802 bits (110), Expect = 8.558e-45
Identity = 31/83 (37.35%), Postives = 45/83 (54.22%), Query Frame = 3

Query:  492 GKDMTKVKHGIHVVSGTPGKGRDLITRKPCRTTTS*FLVADNLLKMLPPGSTDRCAYAVRSLRAGPPAG--VALRRPLPPEVM 734
            G+D+ K+ +G HVV+GTPG+  D+I R+  RT     LV D   +ML  G  ++     R L   PPA   V +   LP E++
Sbjct:  146 GEDIRKLDYGQHVVAGTPGRVFDMIRRRSLRTRAIKMLVLDEADEMLNKGFKEQIYDVYRYL---PPATQVVLISATLPHEIL 225

[Close]

BLAST of EY720867 vs. ExPASy Swiss-Prot
Match: IF4A3_RAT (Eukaryotic initiation factor 4A-III OS=Rattus norvegicus GN=Eif4a3 PE=1 SV=1)

HSP 1 Score: 155.221 bits (391), Expect = 8.558e-45
Identity = 74/124 (59.68%), Postives = 97/124 (78.23%), Query Frame = 2

Query:  125 KMVFETTEGVEAITSFDAMGIKDDLLRGIYQYGFEKPSAIQQRAVMPIIKGRDVIAQAQSGTGKTSMIALTVCQTVDTSTXEVQALILSPTKELATQTEKVILGNGDFINIHPHACVGGQSVRE 496
            K+ FET+E V+   +FD MG+++DLLRGIY YGFEKPSAIQQRA+  IIKGRDVIAQ+QSGTGKT+  +++V Q +D    E QALIL+PT+ELA Q +K +L  GD++N+  HAC+GG +V E
Sbjct:   25 KVEFETSEEVDVTPTFDTMGLREDLLRGIYAYGFEKPSAIQQRAIKQIIKGRDVIAQSQSGTGKTATFSISVLQCLDIQVRETQALILAPTRELAVQIQKGLLALGDYMNVQCHACIGGTNVGE 148

HSP 2 Score: 46.9802 bits (110), Expect = 8.558e-45
Identity = 31/83 (37.35%), Postives = 45/83 (54.22%), Query Frame = 3

Query:  492 GKDMTKVKHGIHVVSGTPGKGRDLITRKPCRTTTS*FLVADNLLKMLPPGSTDRCAYAVRSLRAGPPAG--VALRRPLPPEVM 734
            G+D+ K+ +G HVV+GTPG+  D+I R+  RT     LV D   +ML  G  ++     R L   PPA   V +   LP E++
Sbjct:  147 GEDIRKLDYGQHVVAGTPGRVFDMIRRRSLRTRAIKMLVLDEADEMLNKGFKEQIYDVYRYL---PPATQVVLISATLPHEIL 226

[Close]

BLAST of EY720867 vs. ExPASy Swiss-Prot
Match: IF4A3_PIG (Eukaryotic initiation factor 4A-III OS=Sus scrofa GN=EIF4A3 PE=2 SV=1)

HSP 1 Score: 155.221 bits (391), Expect = 8.558e-45
Identity = 74/124 (59.68%), Postives = 97/124 (78.23%), Query Frame = 2

Query:  125 KMVFETTEGVEAITSFDAMGIKDDLLRGIYQYGFEKPSAIQQRAVMPIIKGRDVIAQAQSGTGKTSMIALTVCQTVDTSTXEVQALILSPTKELATQTEKVILGNGDFINIHPHACVGGQSVRE 496
            K+ FET+E V+   +FD MG+++DLLRGIY YGFEKPSAIQQRA+  IIKGRDVIAQ+QSGTGKT+  +++V Q +D    E QALIL+PT+ELA Q +K +L  GD++N+  HAC+GG +V E
Sbjct:   25 KVEFETSEEVDVTPTFDTMGLREDLLRGIYAYGFEKPSAIQQRAIKQIIKGRDVIAQSQSGTGKTATFSISVLQCLDIQVRETQALILAPTRELAVQIQKGLLALGDYMNVQCHACIGGTNVGE 148

HSP 2 Score: 46.9802 bits (110), Expect = 8.558e-45
Identity = 31/83 (37.35%), Postives = 45/83 (54.22%), Query Frame = 3

Query:  492 GKDMTKVKHGIHVVSGTPGKGRDLITRKPCRTTTS*FLVADNLLKMLPPGSTDRCAYAVRSLRAGPPAG--VALRRPLPPEVM 734
            G+D+ K+ +G HVV+GTPG+  D+I R+  RT     LV D   +ML  G  ++     R L   PPA   V +   LP E++
Sbjct:  147 GEDIRKLDYGQHVVAGTPGRVFDMIRRRSLRTRAIKMLVLDEADEMLNKGFKEQIYDVYRYL---PPATQVVLISATLPHEIL 226

The following BLAST results are available for this feature:

BLAST of EY720867 vs. ExPASy Swiss-Prot

BLAST of EY720867 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 317


Match Name	E-value	Identity	Description
IF4A3_NICPL	4.955e-58	79.03	Eukaryotic initiation factor 4A-3 OS=Nicotiana plu...	[more]
RH2_ORYSJ	9.162e-57	76.42	DEAD-box ATP-dependent RNA helicase 2 OS=Oryza sat...	[more]
RH2_ARATH	1.194e-56	76.61	DEAD-box ATP-dependent RNA helicase 2 OS=Arabidops...	[more]
RH34_ORYSJ	5.864e-56	74.80	DEAD-box ATP-dependent RNA helicase 34 OS=Oryza sa...	[more]
FAL1_USTMA	3.949e-48	62.10	ATP-dependent RNA helicase FAL1 OS=Ustilago maydis...	[more]
RH34_ARATH	2.523e-47	63.41	DEAD-box ATP-dependent RNA helicase 34 OS=Arabidop...	[more]
IF4A3_CHICK	8.557e-45	59.68	Eukaryotic initiation factor 4A-III OS=Gallus gall...	[more]
IF4A3_TAEGU	8.558e-45	59.68	Eukaryotic initiation factor 4A-III OS=Taeniopygia...	[more]
IF4A3_RAT	8.558e-45	59.68	Eukaryotic initiation factor 4A-III OS=Rattus norv...	[more]
IF4A3_PIG	8.558e-45	59.68	Eukaryotic initiation factor 4A-III OS=Sus scrofa ...	[more]

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Properties


Property Name	Value
Genbank description	CS00-C3-703-015-D10-CT.F Sweet orange fruit, development stadium (4 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY720867 ID=EY720867; Name=EY720867; organism=Citrus sinensis; type=EST; length=869bp
CACGCGTCCGGGAAACTCTCATTGATTCTTTGATTTCGAAGAGTAATTGT
TGCGAATTTCGTGCGAGATGGCGGCGGCGACGGCTATGCGGAGGAGAGGA
GGGGGCGGCGGAATGGATGACGACAAGATGGTGTTCGAAACAACGGAAGG
AGTGGAGGCAATCACGAGCTTCGACGCAATGGGAATAAAAGACGATCTGT
TGCGTGGAATTTATCAGTACGGATTCGAGAAGCCATCGGCCATACAACAG
AGGGCCGTTATGCCAATAATAAAGGGCCGTGATGTAATTGCACAGGCCCA
GTCTGGTACTGGCAAGACCTCTATGATTGCCCTAACTGTGTGCCAGACGG
TCGATACTTCTACCANAGAGGTGCAGGCGTTGATTTTGTCACCTACGAAG
GAGTTGGCGACCCAGACAGAGAAAGTGATTTTGGGCAATGGTGATTTTAT
TAACATACACCCACATGCGTGTGTCGGAGGCCAAAGTGTCCGGGAAGGAT
ATGACGAAAGTAAAGCATGGAATCCATGTGGTGTCTGGAACTCCCGGTAA
AGGCCGGGATCTGATTACAAGGAAACCTTGCCGTACTACAACCTCTTAAT
TCTTGGTCGCTGACAATCTGCTTAAAATGCTGCCCCCAGGGTCCACGGAT
AGGTGTGCGTATGCGGTCCGGTCACTTCGCGCCGGTCCTCCGGCTGGCGT
CGCACTCCGCCGCCCCCTTCCCCCCGAAGTGATGGGAGGGGAGCGCCGAG
GAGCGCGGCGCGGTCCGGCGTCGCGGCCTGGGGGATGGTGTGATTCGTCA
GCTTTCGGGGGAAGGAAGAGAGATGTTGGGGCCATCCGAACGACACCCCC
CCCCCCCCCTCCCCCCGCC