EY720838 | Citrus Genome Database

Overview


Name	EY720838
Unique Name	EY720838
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	898

Libraries


Library Name	Type
Sweet orange fruit, development stadium (4 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY720838 vs. ExPASy Swiss-Prot
Match: CH60A_ARATH (Chaperonin CPN60, mitochondrial OS=Arabidopsis thaliana GN=CPN60 PE=1 SV=2)

HSP 1 Score: 104.375 bits (259), Expect = 1.082e-26
Identity = 62/135 (45.93%), Postives = 89/135 (65.93%), Query Frame = 3

Query:  237 AQPITSFEQIALFGTAAANGDRVIGELIAKAFEKGWGNNLHVLAARKTPPSIELQLYWGMTLDRGYLSPYFITNHKTKECVLRDPYIMILEKKVSNCVFFDLLTEIGPVRRYNGTDVLIIAEDIENDLPSSLVLN 641
            A+ I++ E+IA  GT +ANG+R IGELIAKA EK     +  +   KT  + EL++  GM LDRGY SPYFITN KT++C L DP I+I EKK+S+      + E+   R+     +LI++ED+E+D  ++L+LN
Sbjct:  165 ARMISTSEEIAQVGTISANGEREIGELIAKAMEKVGKEGVITIQDGKTLFN-ELEVVEGMKLDRGYTSPYFITNQKTQKCELDDPLILIHEKKISSINSIVKVLELALKRQ---RPLLIVSEDVESDALATLILN 295

HSP 2 Score: 29.261 bits (64), Expect = 1.082e-26
Identity = 12/22 (54.55%), Postives = 15/22 (68.18%), Query Frame = 1

Query:  661 KACVIKPPXIGESXKAXLRDIA 726
            K C IK P  GE+ KA L+D+A
Sbjct:  302 KVCAIKAPGFGENRKANLQDLA 323

HSP 3 Score: 24.6386 bits (52), Expect = 1.082e-26
Identity = 12/24 (50.00%), Postives = 15/24 (62.50%), Query Frame = 2

Query:  182 DQRQGARMAIDALVGNLYSTTHHI 253
            D R+G  MA+DA+V NL S    I
Sbjct:  145 DLRRGISMAVDAVVTNLKSKARMI 168

HSP 4 Score: 23.0978 bits (48), Expect = 1.082e-26
Identity = 13/25 (52.00%), Postives = 16/25 (64.00%), Query Frame = 2

Query:  785 C*EDTVSKDXTVILLCXAAXDRKSV 859
            C + TVSKD TVIL    A D+K +
Sbjct:  350 CKKVTVSKDDTVIL--DGAGDKKGI 372

[Close]

BLAST of EY720838 vs. ExPASy Swiss-Prot
Match: CH62_CUCMA (Chaperonin CPN60-2, mitochondrial OS=Cucurbita maxima GN=CPN60-2 PE=1 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 1.813e-26
Identity = 61/135 (45.19%), Postives = 90/135 (66.67%), Query Frame = 3

Query:  237 AQPITSFEQIALFGTAAANGDRVIGELIAKAFEKGWGNNLHVLAARKTPPSIELQLYWGMTLDRGYLSPYFITNHKTKECVLRDPYIMILEKKVSNCVFFDLLTEIGPVRRYNGTDVLIIAEDIENDLPSSLVLN 641
            A+ I++ E+IA  GT +ANG+R IGELIAKA EK     +  ++  KT  + EL++  GM LDRGY+SPYFITN K ++C L DP I+I EKK+S+      + E+   R+     +LI++ED+E+D  ++L+LN
Sbjct:  166 ARMISTSEEIAQVGTISANGEREIGELIAKAMEKVGKEGVITISDGKTLFN-ELEVVEGMKLDRGYISPYFITNQKNQKCELDDPLILIHEKKISSINSVVKVLELALKRQ---RPLLIVSEDVESDALATLILN 296

HSP 2 Score: 28.8758 bits (63), Expect = 1.813e-26
Identity = 12/22 (54.55%), Postives = 14/22 (63.64%), Query Frame = 1

Query:  661 KACVIKPPXIGESXKAXLRDIA 726
            K C IK P  GE+ KA L D+A
Sbjct:  303 KVCAIKAPGFGENRKAGLHDLA 324

HSP 3 Score: 24.2534 bits (51), Expect = 1.813e-26
Identity = 13/25 (52.00%), Postives = 17/25 (68.00%), Query Frame = 2

Query:  785 C*EDTVSKDXTVILLCXAAXDRKSV 859
            C + T+SKD TVIL    A D+KS+
Sbjct:  351 CKKITISKDDTVIL--DGAGDKKSI 373

HSP 4 Score: 23.483 bits (49), Expect = 1.813e-26
Identity = 11/24 (45.83%), Postives = 15/24 (62.50%), Query Frame = 2

Query:  182 DQRQGARMAIDALVGNLYSTTHHI 253
            D R+G  MA+D++V NL S    I
Sbjct:  146 DLRRGISMAVDSVVTNLKSRARMI 169

[Close]

BLAST of EY720838 vs. ExPASy Swiss-Prot
Match: CH60B_ARATH (Chaperonin CPN60-like 1, mitochondrial OS=Arabidopsis thaliana GN=At2g33210 PE=1 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 3.034e-26
Identity = 61/135 (45.19%), Postives = 89/135 (65.93%), Query Frame = 3

Query:  237 AQPITSFEQIALFGTAAANGDRVIGELIAKAFEKGWGNNLHVLAARKTPPSIELQLYWGMTLDRGYLSPYFITNHKTKECVLRDPYIMILEKKVSNCVFFDLLTEIGPVRRYNGTDVLIIAEDIENDLPSSLVLN 641
            A+ I++ E+IA  GT +ANGDR IGELIAKA E      +  +   KT  + EL++  GM +DRGY+SPYFITN KT++C L DP I+I EKK+SN    + + ++  +       +LI+AED+E+D  ++L+LN
Sbjct:  166 ARMISTSEEIAQVGTISANGDREIGELIAKAMETVGKEGVITIQDGKTLFN-ELEVVEGMKIDRGYISPYFITNPKTQKCELEDPLILIHEKKISN---INAMVKVLELALKKQRPLLIVAEDVESDALATLILN 296

HSP 2 Score: 28.4906 bits (62), Expect = 3.034e-26
Identity = 11/22 (50.00%), Postives = 14/22 (63.64%), Query Frame = 1

Query:  661 KACVIKPPXIGESXKAXLRDIA 726
            K C +K P  GE+ KA L D+A
Sbjct:  303 KVCAVKAPGFGENRKANLHDLA 324

HSP 3 Score: 23.483 bits (49), Expect = 3.034e-26
Identity = 10/24 (41.67%), Postives = 15/24 (62.50%), Query Frame = 2

Query:  182 DQRQGARMAIDALVGNLYSTTHHI 253
            D R+G ++A+D +V NL S    I
Sbjct:  146 DLRRGIKLAVDTVVTNLQSRARMI 169

HSP 4 Score: 21.557 bits (44), Expect = 3.034e-26
Identity = 11/25 (44.00%), Postives = 17/25 (68.00%), Query Frame = 2

Query:  785 C*EDTVSKDXTVILLCXAAXDRKSV 859
            C + TVSKD TV+L    A D++++
Sbjct:  351 CKKVTVSKDDTVVL--DGAGDKQAI 373

[Close]

BLAST of EY720838 vs. ExPASy Swiss-Prot
Match: CH61_CUCMA (Chaperonin CPN60-1, mitochondrial OS=Cucurbita maxima GN=CPN60-1 PE=1 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 3.929e-26
Identity = 58/135 (42.96%), Postives = 90/135 (66.67%), Query Frame = 3

Query:  237 AQPITSFEQIALFGTAAANGDRVIGELIAKAFEKGWGNNLHVLAARKTPPSIELQLYWGMTLDRGYLSPYFITNHKTKECVLRDPYIMILEKKVSNCVFFDLLTEIGPVRRYNGTDVLIIAEDIENDLPSSLVLN 641
            A+ I++ E+IA  GT +ANG+R IGELIAKA EK     +  ++  KT  + EL++  GM LDRGY+SPYFITN K ++C L DP I+I EKK+S+    + + ++  +       +LI++ED+E++  ++L+LN
Sbjct:  166 ARMISTSEEIAQVGTISANGEREIGELIAKAMEKVGKEGVITISDGKTMDN-ELEVVEGMKLDRGYISPYFITNQKNQKCELDDPLIIIYEKKISS---INAVVKVLELALKKQRPLLIVSEDVESEALATLILN 296

HSP 2 Score: 29.261 bits (64), Expect = 3.929e-26
Identity = 12/22 (54.55%), Postives = 15/22 (68.18%), Query Frame = 1

Query:  661 KACVIKPPXIGESXKAXLRDIA 726
            K C IK P  GE+ KA L+D+A
Sbjct:  303 KVCAIKAPGFGENRKAGLQDLA 324

HSP 3 Score: 23.483 bits (49), Expect = 3.929e-26
Identity = 11/24 (45.83%), Postives = 15/24 (62.50%), Query Frame = 2

Query:  182 DQRQGARMAIDALVGNLYSTTHHI 253
            D R+G  MA+D++V NL S    I
Sbjct:  146 DLRRGISMAVDSVVTNLKSRARMI 169

HSP 4 Score: 23.0978 bits (48), Expect = 3.929e-26
Identity = 12/25 (48.00%), Postives = 17/25 (68.00%), Query Frame = 2

Query:  785 C*EDTVSKDXTVILLCXAAXDRKSV 859
            C + T+SKD TVIL    A D+K++
Sbjct:  351 CKKITISKDDTVIL--DGAGDKKAI 373

[Close]

BLAST of EY720838 vs. ExPASy Swiss-Prot
Match: CH61_MAIZE (Chaperonin CPN60-1, mitochondrial OS=Zea mays GN=CPN60I PE=1 SV=2)

HSP 1 Score: 102.834 bits (255), Expect = 4.113e-25
Identity = 59/135 (43.70%), Postives = 88/135 (65.19%), Query Frame = 3

Query:  237 AQPITSFEQIALFGTAAANGDRVIGELIAKAFEKGWGNNLHVLAARKTPPSIELQLYWGMTLDRGYLSPYFITNHKTKECVLRDPYIMILEKKVSNCVFFDLLTEIGPVRRYNGTDVLIIAEDIENDLPSSLVLN 641
            A+ I++ E+IA  GT +ANG+R IGELIAKA EK     +  +A   T  + EL++  GM LDRGY+SPYFITN KT++C L DP I+I +KKV+N      + ++  +       +LI+AED+E++   +L++N
Sbjct:  168 ARMISTSEEIAQVGTISANGEREIGELIAKAMEKVGKEGVITIADGNTLYN-ELEVVEGMKLDRGYISPYFITNSKTQKCELEDPLILIHDKKVTN---MHAVVKVLEMALKKQKPLLIVAEDVESEALGTLIIN 298

HSP 2 Score: 28.8758 bits (63), Expect = 4.113e-25
Identity = 11/22 (50.00%), Postives = 15/22 (68.18%), Query Frame = 1

Query:  661 KACVIKPPXIGESXKAXLRDIA 726
            K C +K P  GE+ KA L+D+A
Sbjct:  305 KVCAVKAPGFGENRKANLQDLA 326

HSP 3 Score: 24.6386 bits (52), Expect = 4.113e-25
Identity = 14/25 (56.00%), Postives = 17/25 (68.00%), Query Frame = 2

Query:  785 C*EDTVSKDXTVILLCXAAXDRKSV 859
            C + TVSKD TVIL    A D+KS+
Sbjct:  353 CKKVTVSKDDTVIL--DGAGDKKSI 375

[Close]

BLAST of EY720838 vs. ExPASy Swiss-Prot
Match: CH62_MAIZE (Chaperonin CPN60-2, mitochondrial OS=Zea mays GN=CPN60II PE=2 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 1.512e-24
Identity = 58/135 (42.96%), Postives = 87/135 (64.44%), Query Frame = 3

Query:  237 AQPITSFEQIALFGTAAANGDRVIGELIAKAFEKGWGNNLHVLAARKTPPSIELQLYWGMTLDRGYLSPYFITNHKTKECVLRDPYIMILEKKVSNCVFFDLLTEIGPVRRYNGTDVLIIAEDIENDLPSSLVLN 641
            A+ I++ E+IA  GT +ANG+R IGELIAKA EK     +  +A   T  + EL++  GM LDRGY+SPYFITN K ++C L DP I+I +KKV+N      + ++  +       +LI+AED+E++   +L++N
Sbjct:  168 ARMISTSEEIAQVGTISANGEREIGELIAKAMEKVGKEGVITIADGNTLYN-ELEVVEGMKLDRGYISPYFITNSKAQKCELEDPLILIHDKKVTN---MHAVVKVLEMALKKQRPLLIVAEDVESEALGTLIIN 298

HSP 2 Score: 28.8758 bits (63), Expect = 1.512e-24
Identity = 11/22 (50.00%), Postives = 15/22 (68.18%), Query Frame = 1

Query:  661 KACVIKPPXIGESXKAXLRDIA 726
            K C +K P  GE+ KA L+D+A
Sbjct:  305 KVCAVKAPGFGENRKANLQDLA 326

HSP 3 Score: 24.6386 bits (52), Expect = 1.512e-24
Identity = 14/25 (56.00%), Postives = 17/25 (68.00%), Query Frame = 2

Query:  785 C*EDTVSKDXTVILLCXAAXDRKSV 859
            C + TVSKD TVIL    A D+KS+
Sbjct:  353 CKKVTVSKDDTVIL--DGAGDKKSI 375

[Close]

BLAST of EY720838 vs. ExPASy Swiss-Prot
Match: CH60_CHLT3 (60 kDa chaperonin OS=Chloroherpeton thalassium (strain ATCC 35110 / GB-78) GN=groL PE=3 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 6.891e-21
Identity = 63/155 (40.65%), Postives = 94/155 (60.65%), Query Frame = 3

Query:  189 DKGLEWQLMHLWEIYTAQPITSFEQIALFGTAAANGDRVIGELIAKAFEK-GWGNNLHVLAARKTPPSIELQLYWGMTLDRGYLSPYFITNHKTKECVLRDPYIMILEKKVSNCVFFDLLTEIGPV---RRYNGTDVLIIAEDIENDLPSSLVLN 641
            DK +   ++ L +I  ++PI   ++IA  G  +AN D  IG+LIA+A EK G    + V  A+ T    EL++  GM  DRGYLSPYF+TN  + E VL +PYI+I +KK+SN      + E+ P+      +G  +LIIAE+IE +  ++LV+N
Sbjct:  121 DKAVTGVIIELKKI--SRPIADKKEIAQVGCISANNDSEIGDLIAEAMEKVGKDGVITVEEAKGT--ETELKVVEGMQFDRGYLSPYFVTNADSMEAVLDEPYILIYDKKISN------MKELLPILEKTAQSGRPLLIIAEEIEGEALATLVVN 265

HSP 2 Score: 27.335 bits (59), Expect = 6.891e-21
Identity = 11/22 (50.00%), Postives = 13/22 (59.09%), Query Frame = 1

Query:  661 KACVIKPPXIGESXKAXLRDIA 726
            K C +K P  G+  KA L DIA
Sbjct:  272 KVCAVKAPGFGDRRKAMLEDIA 293

[Close]

BLAST of EY720838 vs. ExPASy Swiss-Prot
Match: CH60C_ARATH (Chaperonin CPN60-like 2, mitochondrial OS=Arabidopsis thaliana GN=At3g13860 PE=1 SV=2)

HSP 1 Score: 93.2041 bits (230), Expect = 2.563e-20
Identity = 55/132 (41.67%), Postives = 84/132 (63.64%), Query Frame = 3

Query:  246 ITSFEQIALFGTAAANGDRVIGELIAKAFEKGWGNNLHVLAARKTPPSIELQLYWGMTLDRGYLSPYFITNHKTKECVLRDPYIMILEKKVSNCVFFDLLTEIGPVRRYNGTDVLIIAEDIENDLPSSLVLN 641
            I++ E+I    T +ANG+R IGELIA+A EK     +  +A   T  + EL++  GM L RGY+SPYFIT+ KT++C L +P I+I EKK+S+    + L ++      +   +LI+AED+E+D  + L+LN
Sbjct:  168 ISTPEEITQVATISANGEREIGELIARAMEKVGKEGVITVADGNTLDN-ELEVVEGMKLARGYISPYFITDEKTQKCELENPIILIHEKKISD---INSLLKVLEAAVKSSRPLLIVAEDVESDALAMLILN 295

HSP 2 Score: 26.9498 bits (58), Expect = 2.563e-20
Identity = 11/22 (50.00%), Postives = 14/22 (63.64%), Query Frame = 1

Query:  661 KACVIKPPXIGESXKAXLRDIA 726
            K C IK P  G++ KA L D+A
Sbjct:  302 KVCAIKAPGFGDNRKASLDDLA 323

[Close]

BLAST of EY720838 vs. ExPASy Swiss-Prot
Match: CH60_PROA2 (60 kDa chaperonin OS=Prosthecochloris aestuarii (strain DSM 271 / SK 413) GN=groL PE=3 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 3.337e-20
Identity = 57/138 (41.30%), Postives = 84/138 (60.87%), Query Frame = 3

Query:  237 AQPITSFEQIALFGTAAANGDRVIGELIAKAFEKGWGNNLHVLAARKTPPSIELQLYWGMTLDRGYLSPYFITNHKTKECVLRDPYIMILEKKVSNCVFFDLLTEIGPV---RRYNGTDVLIIAEDIENDLPSSLVLN 641
            ++ IT  ++IA  GT +AN D  IGELIA+A +K   + +  +   K   + EL +  GM  DRGYLSPYF+TN +  +  L DPYI+I +KK+SN      + E+ PV      +G  ++IIAEDIE +  ++LV+N
Sbjct:  135 SRDITGKKEIAQVGTISANNDPEIGELIAEAMDKVGKDGVITVEEAKGMDT-ELTVVEGMQFDRGYLSPYFVTNSEKMDADLEDPYILIYDKKISN------MKELLPVLEKTAQSGRPLMIIAEDIEGEALATLVVN 265

HSP 2 Score: 27.335 bits (59), Expect = 3.337e-20
Identity = 11/22 (50.00%), Postives = 13/22 (59.09%), Query Frame = 1

Query:  661 KACVIKPPXIGESXKAXLRDIA 726
            K C +K P  G+  KA L DIA
Sbjct:  272 KVCAVKAPGFGDRRKAMLEDIA 293

[Close]

BLAST of EY720838 vs. ExPASy Swiss-Prot
Match: CH60_CHLCH (60 kDa chaperonin OS=Chlorobium chlorochromatii (strain CaD3) GN=groL PE=3 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 4.341e-20
Identity = 58/138 (42.03%), Postives = 85/138 (61.59%), Query Frame = 3

Query:  237 AQPITSFEQIALFGTAAANGDRVIGELIAKAFEKGWGNNLHVLAARKTPPSIELQLYWGMTLDRGYLSPYFITNHKTKECVLRDPYIMILEKKVSNCVFFDLLTEIGPV---RRYNGTDVLIIAEDIENDLPSSLVLN 641
            ++ I+S ++IA  GT +AN D  IGELIA+A EK   + +  +   K   + EL++  GM  DRGYLSPYF+TN  T E  L +P I+I +KK+SN      + E+ P+      +G  +LIIAEDIE +  ++LV+N
Sbjct:  135 SRSISSKKEIAQVGTISANNDPEIGELIAEAMEKVGKDGVITVEEAKGMET-ELKVVEGMQFDRGYLSPYFVTNSDTMEAELDNPLILIYDKKISN------MKELLPILEKSAQSGRPLLIIAEDIEGEALATLVVN 265

HSP 2 Score: 27.335 bits (59), Expect = 4.341e-20
Identity = 11/22 (50.00%), Postives = 13/22 (59.09%), Query Frame = 1

Query:  661 KACVIKPPXIGESXKAXLRDIA 726
            K C +K P  G+  KA L DIA
Sbjct:  272 KVCAVKAPGFGDRRKAMLEDIA 293

The following BLAST results are available for this feature:

BLAST of EY720838 vs. ExPASy Swiss-Prot

BLAST of EY720838 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
CH60A_ARATH	1.082e-26	45.93	Chaperonin CPN60, mitochondrial OS=Arabidopsis tha...	[more]
CH62_CUCMA	1.813e-26	45.19	Chaperonin CPN60-2, mitochondrial OS=Cucurbita max...	[more]
CH60B_ARATH	3.034e-26	45.19	Chaperonin CPN60-like 1, mitochondrial OS=Arabidop...	[more]
CH61_CUCMA	3.929e-26	42.96	Chaperonin CPN60-1, mitochondrial OS=Cucurbita max...	[more]
CH61_MAIZE	4.113e-25	43.70	Chaperonin CPN60-1, mitochondrial OS=Zea mays GN=C...	[more]
CH62_MAIZE	1.512e-24	42.96	Chaperonin CPN60-2, mitochondrial OS=Zea mays GN=C...	[more]
CH60_CHLT3	6.891e-21	40.65	60 kDa chaperonin OS=Chloroherpeton thalassium (st...	[more]
CH60C_ARATH	2.563e-20	41.67	Chaperonin CPN60-like 2, mitochondrial OS=Arabidop...	[more]
CH60_PROA2	3.337e-20	41.30	60 kDa chaperonin OS=Prosthecochloris aestuarii (s...	[more]
CH60_CHLCH	4.341e-20	42.03	60 kDa chaperonin OS=Chlorobium chlorochromatii (s...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C3-703-015-A05-CT.F Sweet orange fruit, development stadium (4 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY720838 ID=EY720838; Name=EY720838; organism=Citrus sinensis; type=EST; length=898bp
CACGCGTCCGCAAAATCACAAACGATGTTGCGCCATGCCTCTGACCTCGC
CTCCAAAGTCAGGTTCATTTAACTCGGAGCAGACAGTATACCGCGGCGGA
AGTCACTAAAAGATTTTTTGATTGCACCAATTCTAAAGTTAAAAGGGAGA
ATTTTATATGCGGGAGGATGCGGAAGTGCCAGACCAAAGACAAGGGGCTA
GAATGGCAATTGATGCACTTGTGGGAAATTTATACAGCACAACCCATCAC
ATCATTCGAACAAATAGCCCTGTTTGGAACGGCTGCAGCAAATGGTGACA
GAGTGATTGGCGAGCTCATAGCCAAGGCTTTTGAGAAAGGTTGGGGAAAC
AATCTTCATGTTTTAGCTGCTCGGAAAACACCACCCAGTATTGAACTTCA
GTTGTATTGGGGAATGACCCTAGACAGGGGCTATTTGTCACCTTACTTCA
TCACCAATCATAAGACAAAAGAATGTGTNTTACGAGATCCTTATATCATG
ATCCTTGAGAAAAAGGTTTCGAACTGCGTCTTCTTTGACTTATTGACAGA
GATTGGACCAGTTAGAAGATATAATGGCACAGATGTACTGATTATTGCTG
AAGATATTGAAAATGATCTCCCTAGCAGTCTTGTCTTAAATGGGAGTGGC
TACAGCAACCAAGGCTTGTGTCATAAAACCTCCTGNNATTGGGGAAAGTA
NGAAGGCTANCCTTCGGGACATTGCCCAATCACATATGGGGAAGTCATGA
CANGANNAGATTTTGAATGCGTATCTCTTGGCCATGCTGAGAAGATACGG
TATCAAAGGATCANACGGTTATCCTTCTTTGTANGGCTGCATANGACAGA
AAAAGTGTTACAATGGAGATTCCAATGGAAAATTACAAGATTCCCTGA