EY720522

Overview
NameEY720522
Unique NameEY720522
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length858
Libraries
Library NameType
Sweet orange fruit, development stadium (4 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
EY720522_ssr45 microsatellite EY720522_ssr45:45..54. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig11196 contig Csv1_Contig11196:1..859. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of EY720522 vs. ExPASy Swiss-Prot
Match: PTEN_MOUSE (Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN OS=Mus musculus GN=Pten PE=1 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 9.250e-12
Identity = 33/69 (47.83%), Postives = 45/69 (65.22%), Query Frame = 1 
Query:  586 RHVVSQNKRRYQMXGFDLDMTYITENIIAMGFPAGDMSSGVFGYVEGILRNHMEEVI*SLXTITXGQYK 792
            + +VS+NKRRYQ  GFDLD+TYI  NIIAMGFPA  +        EG+ RN++++V+  L +     YK
Sbjct:    6 KEIVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERL--------EGVYRNNIDDVVRFLDSKHKNHYK 66

HSP 2 Score: 25.409 bits (54), Expect = 9.250e-12
Identity = 8/19 (42.11%), Postives = 13/19 (68.42%), Query Frame = 3 
Query:  774 HXGTVQVYNLCSEKAYDAS 830
            H    ++YNLC+E+ YD +
Sbjct:   61 HKNHYKIYNLCAERHYDTA 79
[Close]
BLAST of EY720522 vs. ExPASy Swiss-Prot
Match: PTEN_HUMAN (Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN OS=Homo sapiens GN=PTEN PE=1 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 9.250e-12
Identity = 33/69 (47.83%), Postives = 45/69 (65.22%), Query Frame = 1 
Query:  586 RHVVSQNKRRYQMXGFDLDMTYITENIIAMGFPAGDMSSGVFGYVEGILRNHMEEVI*SLXTITXGQYK 792
            + +VS+NKRRYQ  GFDLD+TYI  NIIAMGFPA  +        EG+ RN++++V+  L +     YK
Sbjct:    6 KEIVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERL--------EGVYRNNIDDVVRFLDSKHKNHYK 66

HSP 2 Score: 25.409 bits (54), Expect = 9.250e-12
Identity = 8/19 (42.11%), Postives = 13/19 (68.42%), Query Frame = 3 
Query:  774 HXGTVQVYNLCSEKAYDAS 830
            H    ++YNLC+E+ YD +
Sbjct:   61 HKNHYKIYNLCAERHYDTA 79
[Close]
BLAST of EY720522 vs. ExPASy Swiss-Prot
Match: PTEN_CANFA (Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN OS=Canis familiaris GN=PTEN PE=2 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 9.250e-12
Identity = 33/69 (47.83%), Postives = 45/69 (65.22%), Query Frame = 1 
Query:  586 RHVVSQNKRRYQMXGFDLDMTYITENIIAMGFPAGDMSSGVFGYVEGILRNHMEEVI*SLXTITXGQYK 792
            + +VS+NKRRYQ  GFDLD+TYI  NIIAMGFPA  +        EG+ RN++++V+  L +     YK
Sbjct:    6 KEIVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERL--------EGVYRNNIDDVVRFLDSKHKNHYK 66

HSP 2 Score: 25.409 bits (54), Expect = 9.250e-12
Identity = 8/19 (42.11%), Postives = 13/19 (68.42%), Query Frame = 3 
Query:  774 HXGTVQVYNLCSEKAYDAS 830
            H    ++YNLC+E+ YD +
Sbjct:   61 HKNHYKIYNLCAERHYDTA 79
[Close]
BLAST of EY720522 vs. ExPASy Swiss-Prot
Match: PTEN_XENLA (Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN OS=Xenopus laevis GN=pten PE=2 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 2.628e-11
Identity = 33/69 (47.83%), Postives = 44/69 (63.77%), Query Frame = 1 
Query:  586 RHVVSQNKRRYQMXGFDLDMTYITENIIAMGFPAGDMSSGVFGYVEGILRNHMEEVI*SLXTITXGQYK 792
            +  VS+NKRRYQ  GFDLD+TYI  NIIAMGFPA  +        EG+ RN++++V+  L +     YK
Sbjct:    6 KEFVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERL--------EGVYRNNIDDVVRFLDSKHKNHYK 66

HSP 2 Score: 25.409 bits (54), Expect = 2.628e-11
Identity = 8/19 (42.11%), Postives = 13/19 (68.42%), Query Frame = 3 
Query:  774 HXGTVQVYNLCSEKAYDAS 830
            H    ++YNLC+E+ YD +
Sbjct:   61 HKNHYKIYNLCAERHYDTN 79
[Close]
BLAST of EY720522 vs. ExPASy Swiss-Prot
Match: TPTE2_MACFA (Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase TPTE2 OS=Macaca fascicularis GN=TPTE2 PE=2 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 7.390e-11
Identity = 29/57 (50.88%), Postives = 36/57 (63.16%), Query Frame = 1 
Query:  586 RHVVSQNKRRYQMXGFDLDMTYITENIIAMGFPAGDMSSGVFGYVEGILRNHMEEVI 756
            R +VS+NKRRY   GFDLD+TY+TE IIAM FP+    S          RN +EEV+
Sbjct:  264 RRLVSENKRRYTRDGFDLDLTYVTERIIAMSFPSSGRQS--------FYRNPIEEVV 312

HSP 2 Score: 28.8758 bits (63), Expect = 7.390e-11
Identity = 11/17 (64.71%), Postives = 13/17 (76.47%), Query Frame = 3 
Query:  774 HXGTVQVYNLCSEKAYD 824
            H    +VYNLCSE+AYD
Sbjct:  319 HPNHYRVYNLCSERAYD 335
[Close]
BLAST of EY720522 vs. ExPASy Swiss-Prot
Match: TPTE2_HUMAN (Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase TPTE2 OS=Homo sapiens GN=TPTE2 PE=1 SV=2)

HSP 1 Score: 56.9954 bits (136), Expect = 7.406e-11
Identity = 29/57 (50.88%), Postives = 36/57 (63.16%), Query Frame = 1 
Query:  586 RHVVSQNKRRYQMXGFDLDMTYITENIIAMGFPAGDMSSGVFGYVEGILRNHMEEVI 756
            R +VS+NKRRY   GFDLD+TY+TE IIAM FP+    S          RN +EEV+
Sbjct:  202 RRLVSENKRRYTRDGFDLDLTYVTERIIAMSFPSSGRQS--------FYRNPIEEVV 250

HSP 2 Score: 28.8758 bits (63), Expect = 7.406e-11
Identity = 11/17 (64.71%), Postives = 13/17 (76.47%), Query Frame = 3 
Query:  774 HXGTVQVYNLCSEKAYD 824
            H    +VYNLCSE+AYD
Sbjct:  257 HRNHYRVYNLCSERAYD 273
The following BLAST results are available for this feature:
BLAST of EY720522 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 6
Match NameE-valueIdentityDescription
PTEN_MOUSE9.250e-1247.83Phosphatidylinositol-3,4,5-trisphosphate 3-phospha... [more]
PTEN_HUMAN9.250e-1247.83Phosphatidylinositol-3,4,5-trisphosphate 3-phospha... [more]
PTEN_CANFA9.250e-1247.83Phosphatidylinositol-3,4,5-trisphosphate 3-phospha... [more]
PTEN_XENLA2.628e-1147.83Phosphatidylinositol-3,4,5-trisphosphate 3-phospha... [more]
TPTE2_MACFA7.390e-1150.88Phosphatidylinositol-3,4,5-trisphosphate 3-phospha... [more]
TPTE2_HUMAN7.406e-1150.88Phosphatidylinositol-3,4,5-trisphosphate 3-phospha... [more]
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Properties
Property NameValue
Genbank descriptionCS00-C3-703-010-F06-CT.F Sweet orange fruit, development stadium (4 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY720522 ID=EY720522; Name=EY720522; organism=Citrus sinensis; type=EST; length=858bp
CCACGCGTCCGCAAATCACAAAATTGAAACTCTCATCATCGTCATCTCTC
TCTCTGCCTAAAACAAGCCCTAGCTTGGCTTCACCATAATCTTTTGTGTA
ATCCTCTGCAACTATCTCAACTGGACTCCAATTCCAATCCCGTGTTCGTG
AATTTTATCATCAATCACCATTGAGACCTCTAAGGAGCCAGAATGGATTC
TGGATCTGCTGATTCATCAGCTTCGCCTCTTACGAAGGCTTCTGAAGTAA
AACCTTCTCCTACTACAGAATCTGCAACAGATAATTCTGCAAGTGATGCA
CGATCAAAGATTTCTGCTACTGGGATATCATCATGGGCCAAAAGTTTGAA
AATTCCTCAGCCATTTTCTGGCTCACAAGAGGATTCACCAACTGGAAATT
CTGGAAAATCAACTTTTGCACGTCTTACTAGTGGCTTAGGACTGCGCTCA
TCTCCAAAATCTCCTCAGTCAGATGATAGTTCTGAGGGGACTTCAACAGC
TGCACAACCTGGTTTTATTGGATCTATTACCAAGGGCTTGGTTGACTCGT
CTAAGAGTGCAGTGAAGGCTGTACAGGTCAAGGCTCGCCATGTTGTCTCT
CAAAATAAACGAAGATACCAGATGGANGGATTTGATTTGGATATGACATA
TATCACTGAGAATATAATTGCAATGGGATTCCCTGCTGGTGACATGAGCT
CAGGGGTTTTCGGATATGTTGAGGGGATTCTACGAAACCACATGGAAGAA
GTGATTTAATCTTTGANNACTATCACANGGGGACAGTACAAGTGTACAAT
CTTTGTTCAGAGAAGGCTTATGATGCATCGTNATTGANGGGGGAAGGTGC
TANTTTCC
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