EY720398

Overview
NameEY720398
Unique NameEY720398
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length900
Libraries
Library NameType
Sweet orange fruit, development stadium (4 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY720398 vs. ExPASy Swiss-Prot
Match: ERF3_YEAST (Eukaryotic peptide chain release factor GTP-binding subunit OS=Saccharomyces cerevisiae GN=SUP35 PE=1 SV=1)

HSP 1 Score: 129.028 bits (323), Expect = 5.896e-40
Identity = 61/98 (62.24%), Postives = 77/98 (78.57%), Query Frame = 1 
Query:  151 ISDAPGHKSYVPTMISGASPADIAVLLISAPQGQCETGFDKGGHTPEHVMLAETFGVTKLLLVVNKMDDHTVNWSKERYDEIESQMTPFSKASGYNVK 444
            I DAPGHK YV  MI GAS AD+ VL+ISA +G+ ETGF++GG T EH +LA+T GV K+++VVNKMDD TVNWSKERYD+  S ++ F +A GYN+K
Sbjct:  342 ILDAPGHKMYVSEMIGGASQADVGVLVISARKGEYETGFERGGQTREHALLAKTQGVNKMVVVVNKMDDPTVNWSKERYDQCVSNVSNFLRAIGYNIK 439

HSP 2 Score: 46.2098 bits (108), Expect = 5.896e-40
Identity = 20/44 (45.45%), Postives = 24/44 (54.55%), Query Frame = 2 
Query:  449 FLPISGLMGLNMKTRVDNTLCPWWNGPCSFEALDLMEITPPHPN 580
            F+P+SG  G N+K  VD   CPW+ GP   E LD M     H N
Sbjct:  444 FMPVSGYSGANLKDHVDPKECPWYTGPTLLEYLDTMNHVDRHIN 487

HSP 3 Score: 31.5722 bits (70), Expect = 5.896e-40
Identity = 13/32 (40.62%), Postives = 22/32 (68.75%), Query Frame = 2 
Query:   56 AYIMDTPQQDMIQGNTAQVGGAHFESETTRST 151
            +++MDT +++   G T +VG A+FE+E  R T
Sbjct:  310 SWVMDTNKEERNDGKTIEVGKAYFETEKRRYT 341
[Close]
BLAST of EY720398 vs. ExPASy Swiss-Prot
Match: ERF3_CANMA (Eukaryotic peptide chain release factor GTP-binding subunit OS=Candida maltosa GN=SUP35 PE=3 SV=2)

HSP 1 Score: 124.405 bits (311), Expect = 1.996e-36
Identity = 59/96 (61.46%), Postives = 73/96 (76.04%), Query Frame = 1 
Query:  151 ISDAPGHKSYVPTMISGASPADIAVLLISAPQGQCETGFDKGGHTPEHVMLAETFGVTKLLLVVNKMDDHTVNWSKERYDEIESQMTPFSKASGYN 438
            I DAPGHK YV  MI GAS AD+ +L+ISA +G+ ETGF+KGG T EH +LA+T GV K+++VVNKMDD TVNWSKERY E  +++  F K  GYN
Sbjct:  371 ILDAPGHKMYVSEMIGGASQADVGILVISARKGEYETGFEKGGQTREHALLAKTQGVNKIIVVVNKMDDPTVNWSKERYQECTTKLGVFLKGIGYN 466

HSP 2 Score: 40.0466 bits (92), Expect = 1.996e-36
Identity = 18/44 (40.91%), Postives = 24/44 (54.55%), Query Frame = 2 
Query:  452 LPISGLMGLNMKTRVDNTLCPWWNGPCSFEALDLMEITPPHPNG 583
            +P+SG  G  +K RV+   CPW+ GP   E LD M+      NG
Sbjct:  473 MPVSGYTGAGLKDRVNPKDCPWYEGPSLLEYLDNMDTMNRKING 516

HSP 3 Score: 30.4166 bits (67), Expect = 1.996e-36
Identity = 12/32 (37.50%), Postives = 22/32 (68.75%), Query Frame = 2 
Query:   56 AYIMDTPQQDMIQGNTAQVGGAHFESETTRST 151
            +++MDT +++   G T +VG A+FE++  R T
Sbjct:  339 SWVMDTNKEERNDGKTIEVGKAYFETDKRRYT 370
[Close]
BLAST of EY720398 vs. ExPASy Swiss-Prot
Match: ERF3_DEBHA (Eukaryotic peptide chain release factor GTP-binding subunit OS=Debaryomyces hansenii GN=SUP35 PE=3 SV=4)

HSP 1 Score: 114.005 bits (284), Expect = 1.624e-35
Identity = 53/95 (55.79%), Postives = 71/95 (74.74%), Query Frame = 1 
Query:  151 ISDAPGHKSYVPTMISGASPADIAVLLISAPQGQCETGFDKGGHTPEHVMLAETFGVTKLLLVVNKMDDHTVNWSKERYDEIESQMTPFSKASGY 435
            I DAPGHK YV  MI GAS AD+ +L+ISA +G+ ETGF+KGG T EH +LA+T GV K+++VVNKMDD TV W+++RY +  +++  F K  GY
Sbjct:  360 ILDAPGHKMYVSEMIGGASQADVGILVISARKGEYETGFEKGGQTREHALLAKTQGVNKIIVVVNKMDDPTVGWAEDRYKDCITKLGTFLKGIGY 454

HSP 2 Score: 45.8246 bits (107), Expect = 1.624e-35
Identity = 20/45 (44.44%), Postives = 27/45 (60.00%), Query Frame = 2 
Query:  449 FLPISGLMGLNMKTRVDNTLCPWWNGPCSFEALDLMEITPPHPNG 583
            F+P+SG  G  +K RV+   CPW++GP   E LD M+    H NG
Sbjct:  461 FMPVSGYTGAGIKDRVNPKDCPWYSGPSLLEFLDNMKTMQRHING 505

HSP 3 Score: 31.9574 bits (71), Expect = 1.624e-35
Identity = 13/32 (40.62%), Postives = 22/32 (68.75%), Query Frame = 2 
Query:   56 AYIMDTPQQDMIQGNTAQVGGAHFESETTRST 151
            +++MDT +++   G T +VG A+FE+E  R T
Sbjct:  328 SWVMDTNKEERSDGKTIEVGKAYFETEKRRYT 359
[Close]
BLAST of EY720398 vs. ExPASy Swiss-Prot
Match: ERF3B_PONAB (Eukaryotic peptide chain release factor GTP-binding subunit ERF3B OS=Pongo abelii GN=GSPT2 PE=2 SV=1)

HSP 1 Score: 129.413 bits (324), Expect = 4.641e-35
Identity = 61/99 (61.62%), Postives = 78/99 (78.79%), Query Frame = 1 
Query:  151 ISDAPGHKSYVPTMISGASPADIAVLLISAPQGQCETGFDKGGHTPEHVMLAETFGVTKLLLVVNKMDDHTVNWSKERYDEIESQMTPFSKASGYNVKK 447
            I DAPGHKS+VP MI GAS AD+AVL+ISA +G+ ETGF+KGG T EH MLA+T GV  L++++NKMDD TVNWS ERY+E + ++ PF K  G++ KK
Sbjct:  285 ILDAPGHKSFVPNMIGGASQADLAVLVISARKGEFETGFEKGGQTREHAMLAKTAGVKHLIVLINKMDDPTVNWSIERYEECKEKLVPFLKKVGFSPKK 383

HSP 2 Score: 31.5722 bits (70), Expect = 4.641e-35
Identity = 13/26 (50.00%), Postives = 17/26 (65.38%), Query Frame = 2 
Query:  449 FLPISGLMGLNMKTRVDNTLCPWWNG 526
            F+P SGL G N+K + D   CPW+ G
Sbjct:  387 FMPCSGLTGANVKEQSD--FCPWYTG 410

HSP 3 Score: 29.261 bits (64), Expect = 4.641e-35
Identity = 12/32 (37.50%), Postives = 21/32 (65.62%), Query Frame = 2 
Query:   56 AYIMDTPQQDMIQGNTAQVGGAHFESETTRST 151
            ++ +DT Q++  +G T +VG A+FE+E    T
Sbjct:  253 SWALDTNQEERDKGKTVEVGRAYFETERKHFT 284
[Close]
BLAST of EY720398 vs. ExPASy Swiss-Prot
Match: ERF3B_HUMAN (Eukaryotic peptide chain release factor GTP-binding subunit ERF3B OS=Homo sapiens GN=GSPT2 PE=1 SV=2)

HSP 1 Score: 129.413 bits (324), Expect = 4.641e-35
Identity = 61/99 (61.62%), Postives = 78/99 (78.79%), Query Frame = 1 
Query:  151 ISDAPGHKSYVPTMISGASPADIAVLLISAPQGQCETGFDKGGHTPEHVMLAETFGVTKLLLVVNKMDDHTVNWSKERYDEIESQMTPFSKASGYNVKK 447
            I DAPGHKS+VP MI GAS AD+AVL+ISA +G+ ETGF+KGG T EH MLA+T GV  L++++NKMDD TVNWS ERY+E + ++ PF K  G++ KK
Sbjct:  285 ILDAPGHKSFVPNMIGGASQADLAVLVISARKGEFETGFEKGGQTREHAMLAKTAGVKHLIVLINKMDDPTVNWSIERYEECKEKLVPFLKKVGFSPKK 383

HSP 2 Score: 31.5722 bits (70), Expect = 4.641e-35
Identity = 13/26 (50.00%), Postives = 17/26 (65.38%), Query Frame = 2 
Query:  449 FLPISGLMGLNMKTRVDNTLCPWWNG 526
            F+P SGL G N+K + D   CPW+ G
Sbjct:  387 FMPCSGLTGANIKEQSD--FCPWYTG 410

HSP 3 Score: 29.261 bits (64), Expect = 4.641e-35
Identity = 12/32 (37.50%), Postives = 21/32 (65.62%), Query Frame = 2 
Query:   56 AYIMDTPQQDMIQGNTAQVGGAHFESETTRST 151
            ++ +DT Q++  +G T +VG A+FE+E    T
Sbjct:  253 SWALDTNQEERDKGKTVEVGRAYFETERKHFT 284
[Close]
BLAST of EY720398 vs. ExPASy Swiss-Prot
Match: ERF3B_MOUSE (Eukaryotic peptide chain release factor GTP-binding subunit ERF3B OS=Mus musculus GN=Gspt2 PE=1 SV=1)

HSP 1 Score: 128.257 bits (321), Expect = 1.018e-34
Identity = 60/99 (60.61%), Postives = 78/99 (78.79%), Query Frame = 1 
Query:  151 ISDAPGHKSYVPTMISGASPADIAVLLISAPQGQCETGFDKGGHTPEHVMLAETFGVTKLLLVVNKMDDHTVNWSKERYDEIESQMTPFSKASGYNVKK 447
            I DAPGHKS+VP MI GAS AD+AVL+ISA +G+ ETGF+KGG T EH MLA+T GV  L++++NKMDD TV+WS ERY+E + ++ PF K  G++ KK
Sbjct:  289 ILDAPGHKSFVPNMIGGASQADLAVLVISARKGEFETGFEKGGQTREHAMLAKTAGVKYLIVLINKMDDPTVDWSSERYEECKEKLVPFLKKVGFSPKK 387

HSP 2 Score: 31.5722 bits (70), Expect = 1.018e-34
Identity = 13/26 (50.00%), Postives = 17/26 (65.38%), Query Frame = 2 
Query:  449 FLPISGLMGLNMKTRVDNTLCPWWNG 526
            F+P SGL G N+K + D   CPW+ G
Sbjct:  391 FMPCSGLTGANIKEQSD--FCPWYTG 414

HSP 3 Score: 29.261 bits (64), Expect = 1.018e-34
Identity = 12/32 (37.50%), Postives = 21/32 (65.62%), Query Frame = 2 
Query:   56 AYIMDTPQQDMIQGNTAQVGGAHFESETTRST 151
            ++ +DT Q++  +G T +VG A+FE+E    T
Sbjct:  257 SWALDTNQEERDKGKTVEVGRAYFETEKKHFT 288
[Close]
BLAST of EY720398 vs. ExPASy Swiss-Prot
Match: ERF3_PICPI (Eukaryotic peptide chain release factor GTP-binding subunit OS=Pichia pinus GN=SUP2 PE=3 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 6.336e-34
Identity = 53/95 (55.79%), Postives = 72/95 (75.79%), Query Frame = 1 
Query:  151 ISDAPGHKSYVPTMISGASPADIAVLLISAPQGQCETGFDKGGHTPEHVMLAETFGVTKLLLVVNKMDDHTVNWSKERYDEIESQMTPFSKASGY 435
            I DAPGHK Y+  MI GAS AD+ VL+IS+ +G+ E GF++GG + EH +LA+T GV KL++V+NKMDD TVNWSKERY+E  +++  + K  GY
Sbjct:  400 ILDAPGHKLYISEMIGGASQADVGVLVISSRKGEYEAGFERGGQSREHAILAKTQGVNKLVVVINKMDDPTVNWSKERYEECTTKLAMYLKGVGY 494

HSP 2 Score: 40.817 bits (94), Expect = 6.336e-34
Identity = 17/38 (44.74%), Postives = 22/38 (57.89%), Query Frame = 2 
Query:  449 FLPISGLMGLNMKTRVDNTLCPWWNGPCSFEALDLMEI 562
            F+P+SG  G  +K RV     PW+NGP   E LD M +
Sbjct:  501 FMPVSGYTGAGLKERVSQKDAPWYNGPSLLEYLDSMPL 538

HSP 3 Score: 29.6462 bits (65), Expect = 6.336e-34
Identity = 12/32 (37.50%), Postives = 22/32 (68.75%), Query Frame = 2 
Query:   56 AYIMDTPQQDMIQGNTAQVGGAHFESETTRST 151
            ++IMDT +++   G T +VG ++FE++  R T
Sbjct:  368 SWIMDTNKEERNDGKTIEVGKSYFETDKRRYT 399
[Close]
BLAST of EY720398 vs. ExPASy Swiss-Prot
Match: ERF3_CANAL (Eukaryotic peptide chain release factor GTP-binding subunit OS=Candida albicans GN=SUP35 PE=3 SV=1)

HSP 1 Score: 120.553 bits (301), Expect = 2.467e-33
Identity = 57/95 (60.00%), Postives = 71/95 (74.74%), Query Frame = 1 
Query:  151 ISDAPGHKSYVPTMISGASPADIAVLLISAPQGQCETGFDKGGHTPEHVMLAETFGVTKLLLVVNKMDDHTVNWSKERYDEIESQMTPFSKASGY 435
            I DAPGHK YV  MI GAS AD+ +L+ISA +G+ ETGF+KGG T EH +LA+T GV K+++VVNKMDD TV WSKERY E  +++  F K  GY
Sbjct:  374 ILDAPGHKMYVSEMIGGASQADVGILVISARKGEYETGFEKGGQTREHALLAKTQGVNKIIVVVNKMDDSTVGWSKERYQECTTKLGAFLKGIGY 468

HSP 2 Score: 43.5134 bits (101), Expect = 2.467e-33
Identity = 19/45 (42.22%), Postives = 26/45 (57.78%), Query Frame = 2 
Query:  449 FLPISGLMGLNMKTRVDNTLCPWWNGPCSFEALDLMEITPPHPNG 583
            ++P+SG  G  +K RVD   CPW++GP   E LD M+      NG
Sbjct:  475 YMPVSGYTGAGLKDRVDPKDCPWYDGPSLLEYLDNMDTMNRKING 519
[Close]
BLAST of EY720398 vs. ExPASy Swiss-Prot
Match: ERF3_SCHPO (Eukaryotic peptide chain release factor GTP-binding subunit OS=Schizosaccharomyces pombe GN=sup35 PE=1 SV=2)

HSP 1 Score: 108.997 bits (271), Expect = 8.697e-33
Identity = 52/97 (53.61%), Postives = 71/97 (73.20%), Query Frame = 1 
Query:  157 DAPGHKSYVPTMISGASPADIAVLLISAPQGQCETGFDKGGHTPEHVMLAETFGVTKLLLVVNKMDDHTVNWSKERYDEIESQMTPF-SKASGYNVK 444
            DAPGHK YV  MI+GAS ADI VL+ISA +G+ E GF++GG T EH +LA T G+  L++V+NKMD+ +V WS+ERY E   +++ F  + +GYN K
Sbjct:  322 DAPGHKGYVTNMINGASQADIGVLVISARRGEFEAGFERGGQTREHAVLARTQGINHLVVVINKMDEPSVQWSEERYKECVDKLSMFLRRVAGYNSK 418

HSP 2 Score: 46.9802 bits (110), Expect = 8.697e-33
Identity = 18/37 (48.65%), Postives = 26/37 (70.27%), Query Frame = 2 
Query:  446 KFLPISGLMGLNMKTRVDNTLCPWWNGPCSFEALDLM 556
            K++P+S   G N+K RVD+++CPW+ GP   E LD M
Sbjct:  422 KYMPVSAYTGQNVKDRVDSSVCPWYQGPSLLEYLDSM 458

HSP 3 Score: 26.5646 bits (57), Expect = 8.697e-33
Identity = 10/30 (33.33%), Postives = 20/30 (66.67%), Query Frame = 2 
Query:   56 AYIMDTPQQDMIQGNTAQVGGAHFESETTR 145
            ++ +D+  ++  +G T +VG A+FE+E  R
Sbjct:  288 SWALDSTSEEREKGKTVEVGRAYFETEHRR 317
[Close]
BLAST of EY720398 vs. ExPASy Swiss-Prot
Match: ERF3A_MOUSE (Eukaryotic peptide chain release factor GTP-binding subunit ERF3A OS=Mus musculus GN=Gspt1 PE=1 SV=1)

HSP 1 Score: 132.494 bits (332), Expect = 3.237e-30
Identity = 62/99 (62.63%), Postives = 78/99 (78.79%), Query Frame = 1 
Query:  151 ISDAPGHKSYVPTMISGASPADIAVLLISAPQGQCETGFDKGGHTPEHVMLAETFGVTKLLLVVNKMDDHTVNWSKERYDEIESQMTPFSKASGYNVKK 447
            I DAPGHKS+VP MI GAS AD+AVL+ISA +G+ ETGF+KGG T EH MLA+T GV  L++++NKMDD TVNWS ERY+E + ++ PF K  G+N KK
Sbjct:  156 ILDAPGHKSFVPNMIGGASQADLAVLVISARKGEFETGFEKGGQTREHAMLAKTAGVKHLIVLINKMDDPTVNWSNERYEECKEKLVPFLKKVGFNPKK 254
The following BLAST results are available for this feature:
BLAST of EY720398 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 180
Match NameE-valueIdentityDescription
ERF3_YEAST5.896e-4062.24Eukaryotic peptide chain release factor GTP-bindin... [more]
ERF3_CANMA1.996e-3661.46Eukaryotic peptide chain release factor GTP-bindin... [more]
ERF3_DEBHA1.624e-3555.79Eukaryotic peptide chain release factor GTP-bindin... [more]
ERF3B_PONAB4.641e-3561.62Eukaryotic peptide chain release factor GTP-bindin... [more]
ERF3B_HUMAN4.641e-3561.62Eukaryotic peptide chain release factor GTP-bindin... [more]
ERF3B_MOUSE1.018e-3460.61Eukaryotic peptide chain release factor GTP-bindin... [more]
ERF3_PICPI6.336e-3455.79Eukaryotic peptide chain release factor GTP-bindin... [more]
ERF3_CANAL2.467e-3360.00Eukaryotic peptide chain release factor GTP-bindin... [more]
ERF3_SCHPO8.697e-3353.61Eukaryotic peptide chain release factor GTP-bindin... [more]
ERF3A_MOUSE3.237e-3062.63Eukaryotic peptide chain release factor GTP-bindin... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-703-008-H07-CT.F Sweet orange fruit, development stadium (4 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY720398 ID=EY720398; Name=EY720398; organism=Citrus sinensis; type=EST; length=900bp
ACCCCCCCTTAGACCGTACCGGCTCCGGAATCCCTTGGCTCACCCACGCC
GTCGGGCGTATATTATGGACACTCCTCAACAGGACATGATCCAGGGTAAC
ACAGCACAAGTGGGAGGAGCTCACTTTGAATCTGAGACTACTAGATCCAC
ATTTCGGATGCGCCGGGTCACAAGAGTTATGTCCCTACTATGATTAGTGG
GGCATCTCCCGCTGATATTGCTGTACTGCTAATTTCTGCTCCACAAGGAC
AGTGTGAAACTGGGTTTGACAAAGGAGGGCACACCCCCGAACATGTCATG
CTTGCCGAAACCTTCGGCGTGACTAAACTGCTTCTTGTGGTAAATAAAAT
GGATGACCACACTGTGAACTGGTCAAAGGAAAGGTATGATGAGATTGAAT
CACAGATGACACCATTTTCAAAGGCTTCGGGCTACAATGTGAAGAAAGTT
TCTCCCAATATCTGGTTTGATGGGCTTAAACATGAAAACAAGAGTGGACA
ACACCCTTTGTCCATGGTGGAATGGCCCCTGTTCTTTTGAAGCCCTTGAT
CTTATGGAGATAACTCCTCCACATCCTAATGGTTCATGTAGGAATGCCTA
TCATTGACCAATTCACCGACCTTGCACATCGTGGTCACGGCGAACGTCCA
CTCCGTTCCTTTCCCGAGCGTCCGTGGTGGGCTCCGGTCCCCCCCCTGGT
GGCCTTCCCCCCTCTCTCCCGCCCCCCACGCTCCGTACTCTCCCTCGCTC
ATTAGTGCCCTTGCCCGACATTCTTTCTTCTCCTGCCTCTTTGCGGTCCG
TTCTTCCGTTCGCCGGCCGTATTCACCTCCCCCACTCCGGGGCGCATCCA
CCGGCCCCACTCATACACCTCCTTCTCACTCCCCCGCTCGCCCCCCGCGG
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