EY720252

Overview
NameEY720252
Unique NameEY720252
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length894
Libraries
Library NameType
Sweet orange fruit, development stadium (4 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig1673 contig Csv1_Contig1673:10..903. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of EY720252 vs. ExPASy Swiss-Prot
Match: CIPKN_ORYSJ (CBL-interacting protein kinase 23 OS=Oryza sativa subsp. japonica GN=CIPK23 PE=2 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 1.704e-26
Identity = 47/69 (68.12%), Postives = 54/69 (78.26%), Query Frame = 3 
Query:  600 SRTGGASRTRVGKYELGRTLGEGSFAKVKFARNTETGENVAH*ILDKEKGLKHKMIGSD*REIQP*NLL 806
            S +GG  RTRVG+YELGRTLGEG+FAKVKFARN ++GENVA  ILDK+K LKHKMI    REI    L+
Sbjct:    2 SVSGG--RTRVGRYELGRTLGEGTFAKVKFARNADSGENVAIKILDKDKVLKHKMIAQIKREISTMKLI 68

HSP 2 Score: 55.4546 bits (132), Expect = 1.704e-26
Identity = 28/35 (80.00%), Postives = 30/35 (85.71%), Query Frame = 2 
Query:  788 STMKLIRHPNVIRMYEVMASKTKYPLVWEL*LGGE 892
            STMKLIRHPNVIRM+EVMASKTK  +V EL  GGE
Sbjct:   63 STMKLIRHPNVIRMHEVMASKTKIYIVMELVTGGE 97
[Close]
BLAST of EY720252 vs. ExPASy Swiss-Prot
Match: CIPKN_ARATH (CBL-interacting serine/threonine-protein kinase 23 OS=Arabidopsis thaliana GN=CIPK23 PE=1 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 4.022e-25
Identity = 46/69 (66.67%), Postives = 53/69 (76.81%), Query Frame = 3 
Query:  600 SRTGGASRTRVGKYELGRTLGEGSFAKVKFARNTETGENVAH*ILDKEKGLKHKMIGSD*REIQP*NLL 806
            S +GG  RTRVGKYELGRTLGEG+FAKVKFARN E G+NVA  ++DKEK LK+KMI    REI    L+
Sbjct:   20 SSSGG--RTRVGKYELGRTLGEGTFAKVKFARNVENGDNVAIKVIDKEKVLKNKMIAQIKREISTMKLI 86

HSP 2 Score: 51.9878 bits (123), Expect = 4.022e-25
Identity = 26/35 (74.29%), Postives = 28/35 (80.00%), Query Frame = 2 
Query:  788 STMKLIRHPNVIRMYEVMASKTKYPLVWEL*LGGE 892
            STMKLI+HPNVIRM+EVMASKTK   V E   GGE
Sbjct:   81 STMKLIKHPNVIRMFEVMASKTKIYFVLEFVTGGE 115
[Close]
BLAST of EY720252 vs. ExPASy Swiss-Prot
Match: CIPKW_ORYSJ (CBL-interacting protein kinase 32 OS=Oryza sativa subsp. japonica GN=CIPK32 PE=2 SV=2)

HSP 1 Score: 80.4925 bits (197), Expect = 1.610e-23
Identity = 42/62 (67.74%), Postives = 49/62 (79.03%), Query Frame = 3 
Query:  621 RTRVGKYELGRTLGEGSFAKVKFARNTETGENVAH*ILDKEKGLKHKMIGSD*REIQP*NLL 806
            + RVGKYELGRT+GEG+FAKVKFAR+TETG+ VA  ILDKEK LKHKM+    REI    L+
Sbjct:    7 KRRVGKYELGRTIGEGTFAKVKFARDTETGDPVAIKILDKEKVLKHKMVEQIKREISTMKLI 68

HSP 2 Score: 50.447 bits (119), Expect = 1.610e-23
Identity = 24/35 (68.57%), Postives = 28/35 (80.00%), Query Frame = 2 
Query:  788 STMKLIRHPNVIRMYEVMASKTKYPLVWEL*LGGE 892
            STMKLI+HPNV+R+YEVM SKTK  +V E   GGE
Sbjct:   63 STMKLIKHPNVVRIYEVMGSKTKIYIVLEYVTGGE 97
[Close]
BLAST of EY720252 vs. ExPASy Swiss-Prot
Match: CIPKX_ORYSJ (CBL-interacting protein kinase 33 OS=Oryza sativa subsp. japonica GN=CIPK33 PE=2 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 6.001e-23
Identity = 42/62 (67.74%), Postives = 49/62 (79.03%), Query Frame = 3 
Query:  621 RTRVGKYELGRTLGEGSFAKVKFARNTETGENVAH*ILDKEKGLKHKMIGSD*REIQP*NLL 806
            + RVGKYELGRT+GEG+FAKVKFAR+TETG+ VA  ILDKEK LKHKM+    REI    L+
Sbjct:    7 KRRVGKYELGRTIGEGTFAKVKFARDTETGDPVAIKILDKEKVLKHKMVEQIKREISTMKLI 68

HSP 2 Score: 48.521 bits (114), Expect = 6.001e-23
Identity = 23/35 (65.71%), Postives = 27/35 (77.14%), Query Frame = 2 
Query:  788 STMKLIRHPNVIRMYEVMASKTKYPLVWEL*LGGE 892
            STMKLI+HPNV+R+YEVM SKT   +V E   GGE
Sbjct:   63 STMKLIKHPNVVRIYEVMGSKTNIYIVLEYVTGGE 97
[Close]
BLAST of EY720252 vs. ExPASy Swiss-Prot
Match: CIPK3_ARATH (CBL-interacting serine/threonine-protein kinase 3 OS=Arabidopsis thaliana GN=CIPK3 PE=1 SV=2)

HSP 1 Score: 78.9518 bits (193), Expect = 1.322e-22
Identity = 41/56 (73.21%), Postives = 46/56 (82.14%), Query Frame = 3 
Query:  621 RTRVGKYELGRTLGEGSFAKVKFARNTETGENVAH*ILDKEKGLKHKMIGSD*REI 788
            + RVGKYE+GRT+GEG+FAKVKFARN+ETGE VA  ILDKEK LKHKM     REI
Sbjct:    8 KRRVGKYEVGRTIGEGTFAKVKFARNSETGEPVALKILDKEKVLKHKMAEQIRREI 63

HSP 2 Score: 48.9062 bits (115), Expect = 1.322e-22
Identity = 22/35 (62.86%), Postives = 29/35 (82.86%), Query Frame = 2 
Query:  788 STMKLIRHPNVIRMYEVMASKTKYPLVWEL*LGGE 892
            +TMKLI+HPNV+++YEVMASKTK  ++ E   GGE
Sbjct:   64 ATMKLIKHPNVVQLYEVMASKTKIFIILEYVTGGE 98
[Close]
BLAST of EY720252 vs. ExPASy Swiss-Prot
Match: CIPK9_ARATH (CBL-interacting serine/threonine-protein kinase 9 OS=Arabidopsis thaliana GN=CIPK9 PE=1 SV=2)

HSP 1 Score: 78.1814 bits (191), Expect = 3.788e-22
Identity = 40/64 (62.50%), Postives = 48/64 (75.00%), Query Frame = 3 
Query:  615 ASRTRVGKYELGRTLGEGSFAKVKFARNTETGENVAH*ILDKEKGLKHKMIGSD*REIQP*NLL 806
            ASRTRVG YE+GRTLGEGSFAKVK+A+NT TG+  A  ILD+EK  +HKM+    REI    L+
Sbjct:   11 ASRTRVGNYEMGRTLGEGSFAKVKYAKNTVTGDQAAIKILDREKVFRHKMVEQLKREISTMKLI 74

HSP 2 Score: 48.1358 bits (113), Expect = 3.788e-22
Identity = 24/35 (68.57%), Postives = 28/35 (80.00%), Query Frame = 2 
Query:  788 STMKLIRHPNVIRMYEVMASKTKYPLVWEL*LGGE 892
            STMKLI+HPNV+ + EVMASKTK  +V EL  GGE
Sbjct:   69 STMKLIKHPNVVEIIEVMASKTKIYIVLELVNGGE 103
[Close]
BLAST of EY720252 vs. ExPASy Swiss-Prot
Match: CIPKQ_ARATH (CBL-interacting serine/threonine-protein kinase 26 OS=Arabidopsis thaliana GN=CIPK26 PE=2 SV=2)

HSP 1 Score: 72.0182 bits (175), Expect = 1.158e-20
Identity = 38/54 (70.37%), Postives = 43/54 (79.63%), Query Frame = 3 
Query:  627 RVGKYELGRTLGEGSFAKVKFARNTETGENVAH*ILDKEKGLKHKMIGSD*REI 788
            RVGKYE+G+TLG+G+FAKV+ A NTETGE VA  ILDKEK LKHKM     REI
Sbjct:    9 RVGKYEVGKTLGQGTFAKVRCAVNTETGERVALKILDKEKVLKHKMAEQIRREI 62

HSP 2 Score: 49.2914 bits (116), Expect = 1.158e-20
Identity = 23/34 (67.65%), Postives = 27/34 (79.41%), Query Frame = 2 
Query:  791 TMKLIRHPNVIRMYEVMASKTKYPLVWEL*LGGE 892
            TMKLI HPNV+R+YEV+ASKTK  +V E   GGE
Sbjct:   64 TMKLINHPNVVRLYEVLASKTKIYIVLEFGTGGE 97
[Close]
BLAST of EY720252 vs. ExPASy Swiss-Prot
Match: CIPK8_ORYSJ (CBL-interacting protein kinase 8 OS=Oryza sativa subsp. japonica GN=CIPK8 PE=2 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 3.313e-20
Identity = 37/60 (61.67%), Postives = 47/60 (78.33%), Query Frame = 3 
Query:  609 GGASRTRVGKYELGRTLGEGSFAKVKFARNTETGENVAH*ILDKEKGLKHKMIGSD*REI 788
            GG +  RVGKYE+GRT+GEG+FAKVKFA+NTE+GE+VA  ++D+   LKHKM     REI
Sbjct:    3 GGGALRRVGKYEVGRTIGEGTFAKVKFAQNTESGESVAMKVVDRSSILKHKMADQIKREI 62

HSP 2 Score: 43.8986 bits (102), Expect = 3.313e-20
Identity = 19/35 (54.29%), Postives = 27/35 (77.14%), Query Frame = 2 
Query:  788 STMKLIRHPNVIRMYEVMASKTKYPLVWEL*LGGE 892
            S MKL+RHPNV+R++EV+AS+ K  ++ E   GGE
Sbjct:   63 SIMKLVRHPNVVRLHEVLASRKKIFIILEFITGGE 97
[Close]
BLAST of EY720252 vs. ExPASy Swiss-Prot
Match: CIPK9_ORYSJ (CBL-interacting protein kinase 9 OS=Oryza sativa subsp. japonica GN=CIPK9 PE=2 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 2.084e-19
Identity = 39/79 (49.37%), Postives = 52/79 (65.82%), Query Frame = 3 
Query:  573 AKIEGKDMASRTGGASRT-RVGKYELGRTLGEGSFAKVKFARNTETGENVAH*ILDKEKGLKHKMIGSD*REIQP*NLL 806
            A+ E     +  G A RT RVG+YELG+T+GEGSFAKVK AR+T TG+ +A  +LD+   L+HKM+    REI    L+
Sbjct:    4 AEAEAAGAGAGAGPARRTTRVGRYELGKTIGEGSFAKVKVARDTRTGDTLAIKVLDRNHVLRHKMVEQIKREISTMKLI 82

HSP 2 Score: 46.2098 bits (108), Expect = 2.084e-19
Identity = 22/35 (62.86%), Postives = 29/35 (82.86%), Query Frame = 2 
Query:  788 STMKLIRHPNVIRMYEVMASKTKYPLVWEL*LGGE 892
            STMKLI+HPNV++++EVMASK+K  +V E   GGE
Sbjct:   77 STMKLIKHPNVVQLHEVMASKSKIYMVLEYVDGGE 111
[Close]
BLAST of EY720252 vs. ExPASy Swiss-Prot
Match: CIPK8_ARATH (CBL-interacting serine/threonine-protein kinase 8 OS=Arabidopsis thaliana GN=CIPK8 PE=1 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 4.587e-19
Identity = 35/54 (64.81%), Postives = 44/54 (81.48%), Query Frame = 3 
Query:  627 RVGKYELGRTLGEGSFAKVKFARNTETGENVAH*ILDKEKGLKHKMIGSD*REI 788
            +VGKYELGRT+GEG+FAKVKFA+NTETGE+VA  I+D+   +K KM+    REI
Sbjct:    5 KVGKYELGRTIGEGTFAKVKFAQNTETGESVAMKIVDRSTIIKRKMVDQIKREI 58

HSP 2 Score: 44.669 bits (104), Expect = 4.587e-19
Identity = 20/35 (57.14%), Postives = 27/35 (77.14%), Query Frame = 2 
Query:  788 STMKLIRHPNVIRMYEVMASKTKYPLVWEL*LGGE 892
            S MKL+RHP V+R+YEV+AS+TK  ++ E   GGE
Sbjct:   59 SIMKLVRHPCVVRLYEVLASRTKIYIILEYITGGE 93
The following BLAST results are available for this feature:
BLAST of EY720252 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 38
Match NameE-valueIdentityDescription
CIPKN_ORYSJ1.704e-2668.12CBL-interacting protein kinase 23 OS=Oryza sativa ... [more]
CIPKN_ARATH4.022e-2566.67CBL-interacting serine/threonine-protein kinase 23... [more]
CIPKW_ORYSJ1.610e-2367.74CBL-interacting protein kinase 32 OS=Oryza sativa ... [more]
CIPKX_ORYSJ6.001e-2367.74CBL-interacting protein kinase 33 OS=Oryza sativa ... [more]
CIPK3_ARATH1.322e-2273.21CBL-interacting serine/threonine-protein kinase 3 ... [more]
CIPK9_ARATH3.788e-2262.50CBL-interacting serine/threonine-protein kinase 9 ... [more]
CIPKQ_ARATH1.158e-2070.37CBL-interacting serine/threonine-protein kinase 26... [more]
CIPK8_ORYSJ3.313e-2061.67CBL-interacting protein kinase 8 OS=Oryza sativa s... [more]
CIPK9_ORYSJ2.084e-1949.37CBL-interacting protein kinase 9 OS=Oryza sativa s... [more]
CIPK8_ARATH4.587e-1964.81CBL-interacting serine/threonine-protein kinase 8 ... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-703-004-A10-CT.F Sweet orange fruit, development stadium (4 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY720252 ID=EY720252; Name=EY720252; organism=Citrus sinensis; type=EST; length=894bp
CCACGCGTCCGGACACCAAATACTTCGCCTACGATCCAGCTCCCAAAATT
TCTTCGCAAGAATCTCCTCAATAATTCACGTAGATGTACGTCTCTGATTT
ATCTTCTCAGTGCAAGCCCCTTTATAGATCCGTGCGCTAATTAATTATTA
TGATTATTATTTCTTTATTCGACTGAAATTTGCGAGTAAATACTTCGATT
TTGCCAAAATCATAATTTACATCCTTTCTCGGCTGATTCACTCGATTCCA
TGGGGTCCATGTCTGATCTGTCGGTCACAATCTCTCATCGATTCGTCCTG
AATTGTCGAGGTTTGAGTTTTTGAATTTTGCGGAGGATCTCCACTGATTT
AGTAATTAGCGTCTGAATAATTTATAAATCTTTTTCTTTTTTTTAAAAAA
AAGAAGCAAAGATGAGGGTAGAGAAGTCGCAGGTATGGCAGCCGTGCAAA
AAGAAGAGGTCTTGATCGTTAGTAAATAATCTCCAAAGCGAGAGAGAAAA
AATCGAAAATAAGAAGAGATTTTTAAATTATATTGAGGAAGGGAAGGGAA
CGAAAACTGAATCTTAGGTTGAGCGAAGATTGAAGGGAAGGACATGGCTT
CTCGCACAGGCGGAGCAAGCAGGACACGTGTTGGGAAGTACGAGTTAGGA
AGGACGCTCGGAGAAGGAAGCTTCGCCAAAGTGAAGTTTGCTAGAAACAC
TGAAACTGGAGAAAATGTCGCCCATTAAATTCTCGATAAAGAAAAAGGTC
TCAAGCATAAGATGATCGGGTCAGATTAGCGTGAAATTCAACCATGAAAC
TTATTAGACACCCTAATGTCATCCGCATGTATGAGGTGATGGCAAGCAAG
ACAAAATATCCATTGGTTTGGGAATTGTGACTGGGTGGTGAACT
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