EY720079

BLAST of EY720079 vs. ExPASy Swiss-Prot
Match: T5H_TAXCU (Taxadiene 5-alpha hydroxylase OS=Taxus cuspidata PE=1 SV=2)

HSP 1 Score: 103.99 bits (258), Expect = 1.246e-21
Identity = 66/190 (34.74%), Postives = 98/190 (51.58%), Query Frame = 3

Query:  138 LPLPPGHVSWPFKYFETLDYLKKARTNTIHKFIAERVQKYKTKCFKTCHIGQNMVFLTSAEGNKFLFSNDYKLVRSWWPVTFLKVF-ENAGEEITVEEVIRARKQFLSFFNEPDALARHVAITDQVVQDHFKCYWDGSKQVGVYPLARKLTFDVSCRLLADIXDREILDESLPLMGNVVRAFFASPITXP 704
            L LPPG +  PF   E+  +L+  R+N++ +F  ERV+K+    FKT  IG   V L    GN+ + SN+ KLV+  WP  F+K+  EN+      E+ I  R     FF  P AL  ++   +  +Q H    W G  +V V PL R+L F++S  L  +I D++  D    L+  ++   FA PI  P
Sbjct:   50 LKLPPGKLGIPF-IGESFIFLRALRSNSLEQFFDERVKKFGL-VFKTSLIGHPTVVLCGPAGNRLILSNEEKLVQMSWPAQFMKLMGENSVATRRGEDHIVMRSALAGFFG-PGALQSYIGKMNTEIQSHINEKWKGKDEVNVLPLVRELVFNISAILFFNIYDKQEQDRLHKLLETILVGSFALPIDLP 236          

BLAST of EY720079 vs. ExPASy Swiss-Prot
Match: C16B1_PICSI (Cytochrome P450 716B1 OS=Picea sitchensis GN=CYP716B1 PE=2 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 1.684e-18
Identity = 60/187 (32.09%), Postives = 88/187 (47.06%), Query Frame = 3

Query:  144 LPPGHVSWPFKYFETLDYLKKARTNTIHKFIAERVQKYKTKCFKTCHIGQNMVFLTSAEGNKFLFSNDYKLVRSWWPVTFLKVFENAGEEITVEEVIRARKQFLSFFNEPDALARHVAITDQVVQDHFKCYWDGSKQVGVYPLARKLTFDVSCRLLADIXDREILDESLPLMGNVVRAFFASPITXP 704
            +PPG   WP    ETL++L   R      F   R QKY    F T  +G   V   S EGN+FLFSN+ KLV + WP +   +F ++      ++  R R+  ++F   P+AL   V   D + + H   +W G  +V   PL ++ TF ++C L A I  ++ LD         V+     PI  P
Sbjct:   51 IPPGTFGWPL-IGETLEFLGCQRKGNPRDFFDSRTQKYGN-VFTTSLVGHPTVVFCSPEGNRFLFSNENKLVVNSWPSSVGNLFRSSLITTVGDDAKRLRRILMTFLR-PEALREFVGRVDSMTKRHLAEHWIGKDEVMALPLLKRYTFSLACDLFASINTKDDLDRLWLHFMVFVKGVMQIPIDLP 234          

BLAST of EY720079 vs. ExPASy Swiss-Prot
Match: C16B2_PICSI (Cytochrome P450 716B2 OS=Picea sitchensis GN=CYP716B2 PE=2 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 4.901e-18
Identity = 55/165 (33.33%), Postives = 83/165 (50.30%), Query Frame = 3

Query:  144 LPPGHVSWPFKYFETLDYLKKARTNTIHKFIAERVQKYKTKCFKTCHIGQNMVFLTSAEGNKFLFSNDYKLVRSWWPVTFLKVFENAGEEITVEEVIRARKQFLSFFNEPDALARHVAITDQVVQDHFKCYWDGSKQVGVYPLARKLTFDVSCRLLADIXDREIL 638
            +PPG   WP    ETL++L   R      F   R QKY    F T  +G   V   S EGN+FLFSN+ KLV + WP +   +F ++      ++  R R+  ++F   P+AL   V   D + + H   +W G  +V   PL ++ TF ++C L A I +++ L
Sbjct:   51 IPPGTFGWPL-IGETLEFLGCQRKGNPRDFFDSRTQKYGN-VFTTSLVGHPTVVFCSPEGNRFLFSNENKLVVNSWPSSVGNLFRSSLITTVGDDAKRLRRILMTFLR-PEALREFVGRVDSMTKRHLAEHWIGKDEVTALPLLKRYTFSLACDLFASINNKDDL 212          

BLAST of EY720079 vs. ExPASy Swiss-Prot
Match: T13H_TAXCU (Taxane 13-alpha-hydroxylase OS=Taxus cuspidata GN=CYP725A2 PE=1 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 2.432e-17
Identity = 61/200 (30.50%), Postives = 99/200 (49.50%), Query Frame = 3

Query:  114 NKQNGSEKLPLPPGHVSWPFKYFETLDYLKKARTNTIHKFIAERVQKYKTKCFKTCHIGQNMVFLTSAEGNKFLFSNDYKLVRSWWPVTFLKVFEN---AGEEITVEEVIRARKQFLSFFNEPDALARHVAITDQVVQDHFKCYWDGSKQVGVYPLARKLTFDVSCRLLADIXDREILDESLPLMGNVVRAFFASPITXP 704
            +K++ S KLP  PG++ +P    ETL +++   ++T  +FI ER+ K+    FKT  IG   V L    GN+ + SN+ KLV+  WP + +K+       G+      ++RA    L+ F  P AL  H A     +Q H    W G  +  V PL + L F V+ RL   I +  + ++   L+  ++   F+ P+  P
Sbjct:   30 SKRHSSVKLP--PGNLGFPL-VGETLQFVRSLGSSTPQQFIEERMSKFGD-VFKTSIIGHPTVVLCGPAGNRLVLSNENKLVQMSWPSSMMKLIGEDCLGGKTGEQHRIVRAA---LTRFLGPQALQNHFAKMSSGIQRHINEKWKGKDEATVLPLVKDLVFSVASRLFFGITEEHLQEQLHNLLEVILVGSFSVPLNIP 222          

BLAST of EY720079 vs. ExPASy Swiss-Prot
Match: T10H_TAXCU (Taxane 10-beta-hydroxylase OS=Taxus cuspidata GN=CYP725A1 PE=1 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 2.783e-13
Identity = 57/199 (28.64%), Postives = 90/199 (45.23%), Query Frame = 3

Query:  117 KQNGSEKLPLPPGHVSWPFKYFETLDYLKKARTNTIHKFIAERVQKYKTKCFKTCHIGQNMVFLTSAEGNKFLFSNDYKLVRSWWPVTFLKVFENAGEEITVE---EVIRARKQFLSFFNEPDALARHVAITDQVVQDHFKCYWDGSKQVGVYPLARKLTFDVSCRLLADIXDREILDESLPLMGNVVRAFFASPITXP 704
            + N    + LPPG + +P    ET+  L+  R+ T  KF  +R++K+    + T  IG   V L    GNK + SN+ KLV    P +F+K+    GE+  V    E  R  +  L+ F    AL  ++      +  HF   W G  +V V PL R L F ++  L  D+ D     +   L+  ++    + P+  P
Sbjct:   41 RYNHRSSVKLPPGKLGFPL-IGETIQLLRTLRSETPQKFFDDRLKKFGP-VYMTSLIGHPTVVLCGPAGNKLVLSNEDKLVEMEGPKSFMKLI---GEDSIVAKRGEDHRILRTALARFLGAQALQNYLGRMSSEIGHHFNEKWKGKDEVKVLPLVRGLIFSIASTLFFDVNDGHQQKQLHHLLETILVGSLSVPLDFP 234