EY693163

Overview
NameEY693163
Unique NameEY693163
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length915
Libraries
Library NameType
Sweet orange fruit, development stadium (1 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY693163 vs. ExPASy Swiss-Prot
Match: NFS1_ARATH (Cysteine desulfurase 1, mitochondrial OS=Arabidopsis thaliana GN=NIFS PE=1 SV=1)

HSP 1 Score: 239.965 bits (611), Expect = 2.387e-72
Identity = 118/184 (64.13%), Postives = 140/184 (76.09%), Query Frame = 3 
Query:   66 MASKLIASTLRXXXXXXXXXXGVRSLSNATAAAVSTAYEPQEDQNGITLKGVRIAGRPLYLDMQATTPVDPRVLDAMLPFYLSRYGNPHSRTHLFGWESEVAVETARSQIADLIGASPKEIIFTSGATESNNVSIKGVMHFYKDKKRHVITTQTEHKCVLDSCRHLQQEGFEITYLPVGADGIV 617
            MASK+I++T+R                +  AAA    YE +     I +KGVRI+GRPLYLDMQATTP+DPRV DAM    +  YGNPHSRTHL+GWE+E AVE AR+Q+A LI ASPKEI+F SGATE+NN+++KGVMHFYKD K+HVITTQTEHKCVLDSCRHLQQEGFE+TYLPV  DG+V
Sbjct:    1 MASKVISATIRRTLTKPHGTFSRCRYLSTAAAATEVNYEDES----IMMKGVRISGRPLYLDMQATTPIDPRVFDAMNASQIHEYGNPHSRTHLYGWEAENAVENARNQVAKLIEASPKEIVFVSGATEANNMAVKGVMHFYKDTKKHVITTQTEHKCVLDSCRHLQQEGFEVTYLPVKTDGLV 180

HSP 2 Score: 54.6842 bits (130), Expect = 2.387e-72
Identity = 25/34 (73.53%), Postives = 29/34 (85.29%), Query Frame = 2 
Query:  620 LDELRAAIXPDTGLISVMGGNNEIGVIQPMEEIG 721
            L+ LR AI PDTGL+S+M  NNEIGV+QPMEEIG
Sbjct:  182 LEMLREAIRPDTGLVSIMAVNNEIGVVQPMEEIG 215
[Close]
BLAST of EY693163 vs. ExPASy Swiss-Prot
Match: NFS1_DROME (Probable cysteine desulfurase, mitochondrial OS=Drosophila melanogaster GN=CG12264 PE=2 SV=1)

HSP 1 Score: 216.853 bits (551), Expect = 6.178e-61
Identity = 104/158 (65.82%), Postives = 124/158 (78.48%), Query Frame = 3 
Query:  144 SNATAAAVSTAYEPQEDQNGITLKGVRIAGRPLYLDMQATTPVDPRVLDAMLPFYLSRYGNPHSRTHLFGWESEVAVETARSQIADLIGASPKEIIFTSGATESNNVSIKGVMHFYKDKKRHVITTQTEHKCVLDSCRHLQQEGFEITYLPVGADGIV 617
            S   A   +T+ E +E Q    +K  +  GRPLYLD QATTP+DPRVLDAMLP+  + YGNPHSRTH +GWE+E AVE AR Q+A LIGA PKEIIFTSGATESNN+++KGV  FY  KKRHVITTQTEHKCVLDSCR L+ EGF++TYLPV A+G++
Sbjct:   32 SRTAAKPAATSKEFRERQVRFNIKNEQTEGRPLYLDAQATTPMDPRVLDAMLPYLTNFYGNPHSRTHAYGWETESAVEKAREQVATLIGADPKEIIFTSGATESNNIAVKGVARFYGTKKRHVITTQTEHKCVLDSCRALENEGFKVTYLPVLANGLI 189

HSP 2 Score: 39.6614 bits (91), Expect = 6.178e-61
Identity = 18/35 (51.43%), Postives = 25/35 (71.43%), Query Frame = 2 
Query:  620 LDELRAAIXPDTGLISVMGGNNEIGVIQPMEEIGQ 724
            L +L   I  +T L+S+M  NNEIGV QP++EIG+
Sbjct:  191 LQQLEETITSETSLVSIMTVNNEIGVRQPVDEIGK 225
[Close]
BLAST of EY693163 vs. ExPASy Swiss-Prot
Match: NFS1_PONAB (Cysteine desulfurase, mitochondrial OS=Pongo abelii GN=NFS1 PE=2 SV=1)

HSP 1 Score: 206.838 bits (525), Expect = 2.328e-60
Identity = 92/128 (71.88%), Postives = 112/128 (87.50%), Query Frame = 3 
Query:  234 RPLYLDMQATTPVDPRVLDAMLPFYLSRYGNPHSRTHLFGWESEVAVETARSQIADLIGASPKEIIFTSGATESNNVSIKGVMHFYKDKKRHVITTQTEHKCVLDSCRHLQQEGFEITYLPVGADGIV 617
            RPLY+D+QATTP+DPRVLDAMLP+ ++ YGNPHSRTH +GWESE A+E AR Q+A LIGA P+EIIFTSGATESNN++IKGV  FY+ +K+H+ITTQTEHKCVLDSCR L+ EGF++TYLPV   GI+
Sbjct:   57 RPLYMDVQATTPLDPRVLDAMLPYLINYYGNPHSRTHAYGWESEAAMERARQQVASLIGADPREIIFTSGATESNNIAIKGVARFYRSRKKHLITTQTEHKCVLDSCRSLEAEGFQVTYLPVQKSGII 184

HSP 2 Score: 47.7506 bits (112), Expect = 2.328e-60
Identity = 24/35 (68.57%), Postives = 27/35 (77.14%), Query Frame = 2 
Query:  620 LDELRAAIXPDTGLISVMGGNNEIGVIQPMEEIGQ 724
            L EL AAI PDT L+SVM  NNEIGV QP+ EIG+
Sbjct:  186 LKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIGR 220
[Close]
BLAST of EY693163 vs. ExPASy Swiss-Prot
Match: NFS1_HUMAN (Cysteine desulfurase, mitochondrial OS=Homo sapiens GN=NFS1 PE=1 SV=3)

HSP 1 Score: 206.838 bits (525), Expect = 2.328e-60
Identity = 92/128 (71.88%), Postives = 112/128 (87.50%), Query Frame = 3 
Query:  234 RPLYLDMQATTPVDPRVLDAMLPFYLSRYGNPHSRTHLFGWESEVAVETARSQIADLIGASPKEIIFTSGATESNNVSIKGVMHFYKDKKRHVITTQTEHKCVLDSCRHLQQEGFEITYLPVGADGIV 617
            RPLY+D+QATTP+DPRVLDAMLP+ ++ YGNPHSRTH +GWESE A+E AR Q+A LIGA P+EIIFTSGATESNN++IKGV  FY+ +K+H+ITTQTEHKCVLDSCR L+ EGF++TYLPV   GI+
Sbjct:   57 RPLYMDVQATTPLDPRVLDAMLPYLINYYGNPHSRTHAYGWESEAAMERARQQVASLIGADPREIIFTSGATESNNIAIKGVARFYRSRKKHLITTQTEHKCVLDSCRSLEAEGFQVTYLPVQKSGII 184

HSP 2 Score: 47.7506 bits (112), Expect = 2.328e-60
Identity = 24/35 (68.57%), Postives = 27/35 (77.14%), Query Frame = 2 
Query:  620 LDELRAAIXPDTGLISVMGGNNEIGVIQPMEEIGQ 724
            L EL AAI PDT L+SVM  NNEIGV QP+ EIG+
Sbjct:  186 LKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIGR 220
[Close]
BLAST of EY693163 vs. ExPASy Swiss-Prot
Match: NFS1_CANMA (Cysteine desulfurase, mitochondrial OS=Candida maltosa GN=SPL1 PE=3 SV=1)

HSP 1 Score: 198.364 bits (503), Expect = 2.719e-60
Identity = 90/128 (70.31%), Postives = 107/128 (83.59%), Query Frame = 3 
Query:  234 RPLYLDMQATTPVDPRVLDAMLPFYLSRYGNPHSRTHLFGWESEVAVETARSQIADLIGASPKEIIFTSGATESNNVSIKGVMHFYKDKKRHVITTQTEHKCVLDSCRHLQQEGFEITYLPVGADGIV 617
            RP+YLD+QATTPVDPRVLD ML FY   YGNPHS TH +GWE++  VE AR  IAD+I A PKEIIFTSGATE+NN++IKGV  FYK  K+H+ITTQTEHKCVLDS RH+Q EGF+ITYLPV ++G++
Sbjct:   83 RPIYLDVQATTPVDPRVLDKMLEFYTGLYGNPHSSTHAYGWETDKEVEKARGYIADVINADPKEIIFTSGATETNNMAIKGVPRFYKKTKKHIITTQTEHKCVLDSARHMQDEGFDITYLPVNSEGLI 210

HSP 2 Score: 50.0618 bits (118), Expect = 2.719e-60
Identity = 24/35 (68.57%), Postives = 29/35 (82.86%), Query Frame = 2 
Query:  620 LDELRAAIXPDTGLISVMGGNNEIGVIQPMEEIGQ 724
            LD+L+ AI  DT L+SVM  NNEIGVIQP++EIGQ
Sbjct:  212 LDDLKKAIRKDTVLVSVMAVNNEIGVIQPLKEIGQ 246

HSP 3 Score: 26.9498 bits (58), Expect = 2.719e-60
Identity = 21/62 (33.87%), Postives = 31/62 (50.00%), Query Frame = 1 
Query:  751 TDAAQAWGRFG*CXKWM*VDVVSGID---YGPK--GACLXIDEARIXXN--LNNGGARKRIR 915
            TDAAQA+G+       M +D++S      YGPK  GAC      R+  +  +  GG  + +R
Sbjct:  257 TDAAQAYGKIPIDVNEMNIDLLSISSHKIYGPKGIGACYVRRRPRVRLDPIITGGGQERGLR 318
[Close]
BLAST of EY693163 vs. ExPASy Swiss-Prot
Match: NFS1_CANAL (Cysteine desulfurase, mitochondrial OS=Candida albicans GN=NFS1 PE=3 SV=1)

HSP 1 Score: 199.519 bits (506), Expect = 4.607e-60
Identity = 90/128 (70.31%), Postives = 108/128 (84.38%), Query Frame = 3 
Query:  234 RPLYLDMQATTPVDPRVLDAMLPFYLSRYGNPHSRTHLFGWESEVAVETARSQIADLIGASPKEIIFTSGATESNNVSIKGVMHFYKDKKRHVITTQTEHKCVLDSCRHLQQEGFEITYLPVGADGIV 617
            RP+YLD+QATTPVDPRVLD ML FY   YGNPHS TH +GWE++  VE AR+ IAD+I A PKEIIFTSGATE+NN++IKGV  FYK  K+H+ITTQTEHKCVLDS RH+Q EGFE+TYLPV ++G++
Sbjct:   87 RPIYLDVQATTPVDPRVLDKMLEFYTGLYGNPHSSTHAYGWETDKEVEKARTYIADVINADPKEIIFTSGATETNNMAIKGVPRFYKKTKKHIITTQTEHKCVLDSARHMQDEGFEVTYLPVSSEGLI 214

HSP 2 Score: 48.1358 bits (113), Expect = 4.607e-60
Identity = 22/35 (62.86%), Postives = 29/35 (82.86%), Query Frame = 2 
Query:  620 LDELRAAIXPDTGLISVMGGNNEIGVIQPMEEIGQ 724
            LD+L+ AI  DT L+S+M  NNEIGVIQP++EIG+
Sbjct:  216 LDDLKKAIRKDTVLVSIMAVNNEIGVIQPLKEIGK 250

HSP 3 Score: 26.9498 bits (58), Expect = 4.607e-60
Identity = 21/62 (33.87%), Postives = 31/62 (50.00%), Query Frame = 1 
Query:  751 TDAAQAWGRFG*CXKWM*VDVVSGID---YGPK--GACLXIDEARIXXN--LNNGGARKRIR 915
            TDAAQA+G+       M +D++S      YGPK  GAC      R+  +  +  GG  + +R
Sbjct:  261 TDAAQAYGKIPIDVNEMNIDLLSISSHKIYGPKGIGACYVRRRPRVRLDPIITGGGQERGLR 322
[Close]
BLAST of EY693163 vs. ExPASy Swiss-Prot
Match: NFS1_RAT (Cysteine desulfurase, mitochondrial OS=Rattus norvegicus GN=Nfs1 PE=2 SV=1)

HSP 1 Score: 203.756 bits (517), Expect = 6.729e-60
Identity = 90/128 (70.31%), Postives = 111/128 (86.72%), Query Frame = 3 
Query:  234 RPLYLDMQATTPVDPRVLDAMLPFYLSRYGNPHSRTHLFGWESEVAVETARSQIADLIGASPKEIIFTSGATESNNVSIKGVMHFYKDKKRHVITTQTEHKCVLDSCRHLQQEGFEITYLPVGADGIV 617
            RPLY+D++ATTP+DPRVLDAMLP+ ++ YGNPHSRTH +GWESE A+E AR Q+A LIGA P+EIIFTSGATESNN++IKGV  FY+ +K+H++TTQTEHKCVLDSCR L+ EGF +TYLPV   GI+
Sbjct:   51 RPLYMDVRATTPLDPRVLDAMLPYLVNYYGNPHSRTHAYGWESEAAMERARQQVASLIGADPREIIFTSGATESNNIAIKGVARFYRSRKKHLVTTQTEHKCVLDSCRSLEAEGFRVTYLPVQKSGII 178

HSP 2 Score: 49.2914 bits (116), Expect = 6.729e-60
Identity = 25/35 (71.43%), Postives = 27/35 (77.14%), Query Frame = 2 
Query:  620 LDELRAAIXPDTGLISVMGGNNEIGVIQPMEEIGQ 724
            L EL AAI PDT L+SVM  NNEIGV QP+ EIGQ
Sbjct:  180 LKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIGQ 214
[Close]
BLAST of EY693163 vs. ExPASy Swiss-Prot
Match: NFS1_MOUSE (Cysteine desulfurase, mitochondrial OS=Mus musculus GN=Nfs1 PE=2 SV=2)

HSP 1 Score: 205.297 bits (521), Expect = 1.945e-59
Identity = 91/128 (71.09%), Postives = 111/128 (86.72%), Query Frame = 3 
Query:  234 RPLYLDMQATTPVDPRVLDAMLPFYLSRYGNPHSRTHLFGWESEVAVETARSQIADLIGASPKEIIFTSGATESNNVSIKGVMHFYKDKKRHVITTQTEHKCVLDSCRHLQQEGFEITYLPVGADGIV 617
            RPLY+D+QATTP+DPRVLDAMLP+ ++ YGNPHSRTH +GWESE A+E AR Q+A LIGA P+EIIFTSGATESNN++IKGV  FY+ +K+H++TTQTEHKCVLDSCR L+ EGF +TYLPV   GI+
Sbjct:   51 RPLYMDVQATTPLDPRVLDAMLPYLVNYYGNPHSRTHAYGWESEAAMERARQQVASLIGADPREIIFTSGATESNNIAIKGVARFYRSRKKHLVTTQTEHKCVLDSCRSLEAEGFRVTYLPVQKSGII 178

HSP 2 Score: 46.2098 bits (108), Expect = 1.945e-59
Identity = 24/35 (68.57%), Postives = 26/35 (74.29%), Query Frame = 2 
Query:  620 LDELRAAIXPDTGLISVMGGNNEIGVIQPMEEIGQ 724
            L EL AAI PDT L+SVM  NNEIGV QP+ EI Q
Sbjct:  180 LKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIRQ 214
[Close]
BLAST of EY693163 vs. ExPASy Swiss-Prot
Match: NFS1_DICDI (Probable cysteine desulfurase, mitochondrial OS=Dictyostelium discoideum GN=nfs1 PE=1 SV=1)

HSP 1 Score: 199.904 bits (507), Expect = 8.677e-57
Identity = 88/135 (65.19%), Postives = 110/135 (81.48%), Query Frame = 3 
Query:  213 KGVRIAGRPLYLDMQATTPVDPRVLDAMLPFYLSRYGNPHSRTHLFGWESEVAVETARSQIADLIGASPKEIIFTSGATESNNVSIKGVMHFYKDKKRHVITTQTEHKCVLDSCRHLQQEGFEITYLPVGADGIV 617
            +G+    +P+YLDMQ+TTP+DPRVLDAMLP Y   YGNPHS+TH +GW S   VE AR +++ +IGA  KEIIFTSGATES N++IKGV  FYK+KK H+ITT TEHKC+LDSCRHL+ EGF++TYLPVG +G+V
Sbjct:   43 RGIAGEKQPIYLDMQSTTPIDPRVLDAMLPLYTENYGNPHSKTHAYGWTSNDLVEDAREKVSKIIGADSKEIIFTSGATESGNIAIKGVARFYKEKKNHIITTVTEHKCILDSCRHLEMEGFKVTYLPVGENGLV 177

HSP 2 Score: 42.743 bits (99), Expect = 8.677e-57
Identity = 18/35 (51.43%), Postives = 27/35 (77.14%), Query Frame = 2 
Query:  620 LDELRAAIXPDTGLISVMGGNNEIGVIQPMEEIGQ 724
            L+ L+  I P T L+++M  NNEIGV+QP++EIG+
Sbjct:  179 LELLKNTITPQTSLVTIMAVNNEIGVVQPIKEIGK 213
[Close]
BLAST of EY693163 vs. ExPASy Swiss-Prot
Match: NFS1_SCHPO (Probable cysteine desulfurase, mitochondrial OS=Schizosaccharomyces pombe GN=SPBC21D10.11c PE=2 SV=1)

HSP 1 Score: 185.652 bits (470), Expect = 4.630e-55
Identity = 86/151 (56.95%), Postives = 112/151 (74.17%), Query Frame = 3 
Query:  183 PQEDQNGITLKGVRIAG------RPLYLDMQATTPVDPRVLDAMLPFYLSRYGNPHSRTHLFGWESEVAVETARSQIADLIGASPKEIIFTSGATESNNVSIKGVMHFYKDKKRHVITTQTEHKCVLDSCRHLQQEGFEITYLPVGADGIV 617
            P+     +TL   + A        P+Y+D QAT+P+D RVLD+MLPF+   YGNPHSRTH +GWE+E AVE AR +IA +I A P+EIIFTSGATESNN  +KGV  FYK +K+H+++ QTEHKCVLDS R LQ+EGFE+T+LPV  +G++
Sbjct:   76 PENSLKTVTLDQAQTAASTVTGLHPIYMDFQATSPLDYRVLDSMLPFFTGIYGNPHSRTHAYGWEAEKAVENARQEIASVINADPREIIFTSGATESNNAILKGVARFYKSRKKHLVSVQTEHKCVLDSLRALQEEGFEVTFLPVQTNGLI 226

HSP 2 Score: 51.2174 bits (121), Expect = 4.630e-55
Identity = 25/35 (71.43%), Postives = 28/35 (80.00%), Query Frame = 2 
Query:  620 LDELRAAIXPDTGLISVMGGNNEIGVIQPMEEIGQ 724
            LDELR AI PDT  +SVM  NNEIGV QP+EEIG+
Sbjct:  228 LDELRDAIRPDTVCVSVMAVNNEIGVCQPLEEIGK 262
The following BLAST results are available for this feature:
BLAST of EY693163 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 203
Match NameE-valueIdentityDescription
NFS1_ARATH2.387e-7264.13Cysteine desulfurase 1, mitochondrial OS=Arabidops... [more]
NFS1_DROME6.178e-6165.82Probable cysteine desulfurase, mitochondrial OS=Dr... [more]
NFS1_PONAB2.328e-6071.88Cysteine desulfurase, mitochondrial OS=Pongo abeli... [more]
NFS1_HUMAN2.328e-6071.88Cysteine desulfurase, mitochondrial OS=Homo sapien... [more]
NFS1_CANMA2.719e-6070.31Cysteine desulfurase, mitochondrial OS=Candida mal... [more]
NFS1_CANAL4.607e-6070.31Cysteine desulfurase, mitochondrial OS=Candida alb... [more]
NFS1_RAT6.729e-6070.31Cysteine desulfurase, mitochondrial OS=Rattus norv... [more]
NFS1_MOUSE1.945e-5971.09Cysteine desulfurase, mitochondrial OS=Mus musculu... [more]
NFS1_DICDI8.677e-5765.19Probable cysteine desulfurase, mitochondrial OS=Di... [more]
NFS1_SCHPO4.630e-5556.95Probable cysteine desulfurase, mitochondrial OS=Sc... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionCS00-C3-700-008-F02-CT.F Sweet orange fruit, development stadium (1 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY693163 ID=EY693163; Name=EY693163; organism=Citrus sinensis; type=EST; length=915bp
CCACGCGTCCGCGGACGCGTGGGTACGCAACGAAGCAAAGCAAAGAGAAA
AAGAAGCCGTTGAAAATGGCCTCTAAGCTAATAGCTTCAACTCTCCGCAA
AACCCTAACTAAGGCCGCCAAGACCGCCGGCGTCCGCTCCCTTTCCAACG
CCACAGCCGCCGCGGTTTCTACGGCGTATGAACCTCAGGAGGACCAAAAC
GGAATCACCTTGAAAGGCGTGAGAATCGCGGGCCGCCCTCTCTACCTCGA
TATGCAAGCAACCACTCCAGTCGACCCACGTGTCCTCGATGCCATGCTCC
CTTTCTATCTTTCTCGTTACGGTAACCCCCACTCCCGGACCCACCTCTTC
GGCTGGGAATCCGAAGTCGCCGTCGAAACCGCCCGCTCTCAAATCGCCGA
TCTTATCGGCGCTTCTCCTAAAGAGATAATTTTCACTTCCGGCGCAACGG
AATCGAATAATGTCTCCATCAAAGGAGTCATGCACTTTTACAAGGATAAA
AAGCGCCACGTCATCACCACTCAGACCGAGCACAAGTGCGTTCTCGATTC
GTGCCGGCATCTTCAGCAGGAAGGTTTTGAAATTACTTATTTACCTGTTG
GAGCTGATGGGATTGTTATTTAGATGAATTGCGAGCTGCTATTANGCCTG
ACACCGGCTTGATTTCTGTTATGGGCGGTAACAACGAAATTGGCGTGATT
CAGCCTATGGAAGAAATCGGCCAATTNGAAAAGATTTAAATCCCGTTTCC
ACTGATGCTGCCCAGGCGTGGGGAAGATTCGGNTGATGTCNAAAATGGAT
GTGAGTTGATGTCGTGAGTGGCATAGATTATGGGCCGAAAGGAGCGTGCT
TGNATATAGACGAGGCCAGAATCNAGNTGAACCTTAATAATGGAGGGGCC
AGAAAAAGGATAAGA
back to top