EY692983

Overview
NameEY692983
Unique NameEY692983
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length922
Libraries
Library NameType
Sweet orange fruit, development stadium (1 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of EY692983 vs. ExPASy Swiss-Prot
Match: SEC14_SCHPO (Sec14 cytosolic factor OS=Schizosaccharomyces pombe GN=sec14 PE=2 SV=1)

HSP 1 Score: 170.629 bits (431), Expect = 1.097e-41
Identity = 90/213 (42.25%), Postives = 129/213 (60.56%), Query Frame = 3
Query:   12 QSKDMWAEMLQWRKEFGVDTIMEDFEFKEINEVLSYYPHGYHGVDKEGRPVYIERLGKVDSNKLMQVTTMDRYIRYHVQGFEKAFAVKFPACTIAAKRHIDSSTSILDVQGVGLKNFSKNARELILRLQKIDGDNYPETLHQMFIINAGPGFRLLWNTVKSFLDPKTTSKIHVLGNKYQSKLLEIIDARELPEFLGGTLYCADQGGCLRSDKG 650
            QS +M+ +  +WRKEFGVD ++++F + E   V  YYP  YH  D +GRPVY+E+LG +D  KL Q+TT +R ++  V  +E     +FPAC+  A   I++S +I+D++GVG+ +   +    I +   I  D YPE + + ++INA  GF   +N +K FLD  T  KIH+LG+ Y+S LLE I A  LP  LGG   C   GGC  SD G
Sbjct:   66 QSLEMFIKCEKWRKEFGVDDLIKNFHYDEKEAVSKYYPQFYHKTDIDGRPVYVEQLGNIDLKKLYQITTPERMMQNLVYEYEMLALKRFPACSRKAGGLIETSCTIMDLKGVGITSI-HSVYSYIRQASSISQDYYPERMGKFYVINAPWGFSSAFNLIKGFLDEATVKKIHILGSNYKSALLEQIPADNLPAKLGGNCQC--PGGCELSDAG 275          
BLAST of EY692983 vs. ExPASy Swiss-Prot
Match: SEC14_YARLI (SEC14 cytosolic factor OS=Yarrowia lipolytica GN=SEC14 PE=3 SV=2)

HSP 1 Score: 161.77 bits (408), Expect = 5.096e-39
Identity = 93/234 (39.74%), Postives = 137/234 (58.55%), Query Frame = 3
Query:    9 PQSKDMWAEMLQWRKEFGVDTIMEDFEFKEINEVLSYYPHGYHGVDKEGRPVYIERLGKVDSNKLMQVTTMDRYIRYHVQGFEKAFAVKFPACTIAAKRHIDSSTSILDVQGVGLKNFSKNARELILRLQKIDGDNYPETLHQMFIINAGPGFRLLWNTVKSFLDPKTTSKIHVLGNKYQSKLLEIIDARELPEFLGGTLYCADQGGCLRSDKGHGKTRDIEMV-LNGRAPRAG 707
            P +++MW    +WRKEFG +TI+EDF +KE  EV   YP  YH  DK+GRPVY+E +GKV+ +++ ++TT +R +R  V  +E     + PAC+      I++S +ILD++GV L + S+     +     I  + YPE + + ++INA  GF  +++ +K FLDP T SKIHV G+ Y+ KLL  + A  LP   GG    + + G   SD   G  RD + V   G AP AG
Sbjct:   69 PLAQEMWENCEKWRKEFGTNTILEDFWYKEKKEVAKLYPQYYHKTDKDGRPVYVENVGKVNIHEMYKITTQERMLRNLVWEYESFVRHRLPACSRVVGHLIETSCTILDLKGVSLSSASQ-VYGFLKDASNIGQNYYPERMGKFYLINAPFGFSTVFSVIKRFLDPVTVSKIHVYGSNYKEKLLAQVPAYNLPIKFGG--QSSSKIGVELSD--DGPWRDPQFVGPEGLAPVAG 297          
BLAST of EY692983 vs. ExPASy Swiss-Prot
Match: SEC14_KLULA (SEC14 cytosolic factor OS=Kluyveromyces lactis GN=SEC14 PE=3 SV=2)

HSP 1 Score: 155.606 bits (392), Expect = 3.652e-37
Identity = 87/231 (37.66%), Postives = 131/231 (56.71%), Query Frame = 3
Query:   15 SKDMWAEMLQWRKEFGVDTIMEDFEFKEINEVLSYYPHGYHGVDKEGRPVYIERLGKVDSNKLMQVTTMDRYIRYHVQGFEKAFAVKFPACTIAAKRHIDSSTSILDVQGVGLKNFSKNARELILRLQKIDGDNYPETLHQMFIINAGPGFRLLWNTVKSFLDPKTTSKIHVLGNKYQSKLLEIIDARELPEFLGGTLYCAD-QGGCLRSDKGHGKTRDIEMVLNGRAPRA 704
            SK M+    +WRKEFGVDTI EDF ++E   V  YYP  YH  D +GRPVYIE LG V+  ++ ++TT +R ++  V  +E     + PAC+  A   +++S +ILD++G+ + + +      +     I  + YPE + + ++INA  GF   +   K FLDP T SKI +LG+ YQ  LL+ I A  LP+  GG    ++ +GG   SD G  +  +  +   G AP+A
Sbjct:   70 SKIMYENCEKWRKEFGVDTIFEDFHYEEKPLVAKYYPQYYHKTDNDGRPVYIEELGSVNLTQMYKITTQERMLKNLVWEYEAFVRYRLPACSRKAGYLVETSCTILDLKGISISS-AAQVLSYVREASNIGQNYYPERMGKFYLINAPFGFSTAFRLFKPFLDPVTVSKIFILGSSYQKDLLKQIPAENLPKKFGGQSEVSEAEGGLYLSDIGPWREEEY-IGPEGEAPKA 298          
BLAST of EY692983 vs. ExPASy Swiss-Prot
Match: SEC14_CANAL (SEC14 cytosolic factor OS=Candida albicans GN=SEC14 PE=2 SV=1)

HSP 1 Score: 153.295 bits (386), Expect = 1.813e-36
Identity = 87/229 (37.99%), Postives = 130/229 (56.77%), Query Frame = 3
Query:   21 DMWAEMLQWRKEFGVDTIMEDFEFKEINEVLSYYPHGYHGVDKEGRPVYIERLGKVDSNKLMQVTTMDRYIRYHVQGFEKAFAVKFPACTIAAKRHIDSSTSILDVQGVGLKNFSKNARELILRLQKIDGDNYPETLHQMFIINAGPGFRLLWNTVKSFLDPKTTSKIHVLGNKYQSKLLEIIDARELPEFLGGTLYCADQGGCLRSDKGHGKTRDIEMV-LNGRAPRA 704
            DM+    +WR++FGV+TI++DF ++E   V   YP  YH  DK+GRPVY E LGKVD  K++++TT +R ++  V  +E     + PAC+  A   +++S ++LD+ G+ + + + N    +    KI  D YPE + + ++INA  GF   +   K FLDP T SKIH+LG  Y+ +LL+ I  + LP   GG    +D    L   K  G  RD E +   G  PRA
Sbjct:   74 DMFVACEKWREDFGVNTILKDFHYEEKPIVAKMYPTYYHKTDKDGRPVYFEELGKVDLVKMLKITTQERMLKNLVWEYEAMCQYRLPACSRKAGYLVETSCTVLDLSGISVTS-AYNVIGYVREASKIGQDYYPERMGKFYLINAPFGFSTAFKLFKPFLDPVTVSKIHILGYSYKKELLKQIPPQNLPVKFGGMSDVSDDDLLL---KDVGPWRDPEFIGPEGECPRA 298          
BLAST of EY692983 vs. ExPASy Swiss-Prot
Match: SEC14_CANGA (SEC14 cytosolic factor OS=Candida glabrata GN=SEC14 PE=3 SV=1)

HSP 1 Score: 150.214 bits (378), Expect = 1.534e-35
Identity = 79/213 (37.09%), Postives = 124/213 (58.22%), Query Frame = 3
Query:   15 SKDMWAEMLQWRKEFGVDTIMEDFEFKEINEVLSYYPHGYHGVDKEGRPVYIERLGKVDSNKLMQVTTMDRYIRYHVQGFEKAFAVKFPACTIAAKRHIDSSTSILDVQGVGLKNFSKNARELILRLQKIDGDNYPETLHQMFIINAGPGFRLLWNTVKSFLDPKTTSKIHVLGNKYQSKLLEIIDARELPEFLGGTLYCAD-QGGCLRSDKG 650
            +K+M+    +WRKE+G +TIM+DF + E   V  YYP  YH  DK+GRPVY E LG V+  ++ ++TT +R ++  V  +E     + PAC+ AA   +++S +++D++G+ + + + +    +     I  + YPE + + ++INA  GF   +   K FLDP T SKI +LG+ YQS+LL+ I A  LP   GG     +  GG   SD G
Sbjct:   70 AKEMFENCEKWRKEYGTNTIMQDFHYDEKPLVAKYYPQYYHKTDKDGRPVYFEELGAVNLTEMEKITTQERMLKNLVWEYESVVNYRLPACSRAAGYLVETSCTVMDLKGISISS-AYSVLSYVREASYISQNYYPERMGKFYLINAPFGFSTAFRLFKPFLDPVTVSKIFILGSSYQSELLKQIPAENLPSKFGGKSEVDEAAGGLYLSDIG 281          
BLAST of EY692983 vs. ExPASy Swiss-Prot
Match: SEC14_YEAST (SEC14 cytosolic factor OS=Saccharomyces cerevisiae GN=SEC14 PE=1 SV=3)

HSP 1 Score: 147.902 bits (372), Expect = 7.615e-35
Identity = 83/232 (35.78%), Postives = 133/232 (57.33%), Query Frame = 3
Query:   15 SKDMWAEMLQWRKEFGVDTIMEDFEFKEINEVLSYYPHGYHGVDKEGRPVYIERLGKVDSNKLMQVTTMDRYIRYHVQGFEKAFAVKFPACTIAAKRHIDSSTSILDVQGVGLKNFSKNARELILRLQKIDGDNYPETLHQMFIINAGPGFRLLWNTVKSFLDPKTTSKIHVLGNKYQSKLLEIIDARELPEFLGGTLYCAD-QGGCLRSDKGHGKTRDIEMV-LNGRAPRA 704
            +K+M+    +WRK++G DTI++DF + E   +  +YP  YH  DK+GRPVY E LG V+ +++ +VT+ +R ++  V  +E     + PAC+ AA   +++S +I+D++G+ + + + +    +     I  + YPE + + +IINA  GF   +   K FLDP T SKI +LG+ YQ +LL+ I A  LP   GG     + +GG   SD   G  RD + +   G AP A
Sbjct:   72 AKEMFENCEKWRKDYGTDTILQDFHYDEKPLIAKFYPQYYHKTDKDGRPVYFEELGAVNLHEMNKVTSEERMLKNLVWEYESVVQYRLPACSRAAGHLVETSCTIMDLKGISISS-AYSVMSYVREASYISQNYYPERMGKFYIINAPFGFSTAFRLFKPFLDPVTVSKIFILGSSYQKELLKQIPAENLPVKFGGKSEVDESKGGLYLSD--IGPWRDPKYIGPEGEAPEA 300          
BLAST of EY692983 vs. ExPASy Swiss-Prot
Match: SEC14_ASHGO (SEC14 cytosolic factor OS=Ashbya gossypii GN=SEC14 PE=3 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 4.179e-33
Identity = 78/213 (36.62%), Postives = 122/213 (57.28%), Query Frame = 3
Query:   15 SKDMWAEMLQWRKEFGVDTIMEDFEFKEINEVLSYYPHGYHGVDKEGRPVYIERLGKVDSNKLMQVTTMDRYIRYHVQGFEKAFAVKFPACTIAAKRHIDSSTSILDVQGVGLKNFSKNARELILRLQKIDGDNYPETLHQMFIINAGPGFRLLWNTVKSFLDPKTTSKIHVLGNKYQSKLLEIIDARELPEFLGGTLYCAD-QGGCLRSDKG 650
            ++ M+    +WRKE GVDTI EDF ++E   V  +YP  YH  DK+GRPVYIE LG V+  ++ ++TT +R ++  +  +E     + PA +  A   +++S +ILD++G+ + + +      +     I  + YPE + + ++INA  GF   +   K FLDP T SKI +LG+ YQ +LL+ I A  LP   GG    ++ +GG   SD G
Sbjct:   73 ARAMFENCEKWRKENGVDTIFEDFHYEEKPLVAKFYPQYYHKTDKDGRPVYIEELGAVNLTEMYKITTQERMLKNLIWEYESFSRYRLPASSRQADCLVETSCTILDLKGISI-SAAAQVLSYVREASNIGQNYYPERMGKFYMINAPFGFSAAFRLFKPFLDPVTVSKIFILGSSYQKELLKQIPAENLPVKFGGQSDVSEAEGGLYLSDIG 284          
BLAST of EY692983 vs. ExPASy Swiss-Prot
Match: YKJ1_YEAST (CRAL-TRIO domain-containing protein YKL091C OS=Saccharomyces cerevisiae GN=YKL091C PE=1 SV=2)

HSP 1 Score: 137.117 bits (344), Expect = 1.344e-31
Identity = 75/203 (36.95%), Postives = 121/203 (59.61%), Query Frame = 3
Query:   15 SKDMWAEMLQWRKEFGVDTIMEDFEF------KEINEVLSYYPHGYHGVDKEGRPVYIERLGKVDSNKLMQVTTMDRYIRYHVQGFEKAFAVKFPACTIAAKRHIDSSTSILDVQGVGLKNFSKNARELILRLQKIDGDNYPETLHQMFIINAGPGFRLLWNTVKSFLDPKTTSKIHVLGNKYQSKLLEIIDARELPEFLGGT 605
            S +M+ E  +WR+E+G +TI+ED+E       KE  ++   YP  YH VDK+GRP+Y E LG ++  K+ ++TT  + +R  V+ +E     + PAC+  A   I++S ++LD++G+ L N + +    I  +  I  + YPE + + +II++  GF  ++  VK FLDP T SKI +LG+ Y+ +LL+ I    LP   GGT
Sbjct:   68 SVEMFVETERWREEYGANTIIEDYENNKEAEDKERIKLAKMYPQYYHHVDKDGRPLYFEELGGINLKKMYKITTEKQMLRNLVKEYELFATYRVPACSRRAGYLIETSCTVLDLKGISLSN-AYHVLSYIKDVADISQNYYPERMGKFYIIHSPFGFSTMFKMVKPFLDPVTVSKIFILGSSYKKELLKQIPIENLPVKYGGT 269          
BLAST of EY692983 vs. ExPASy Swiss-Prot
Match: S14L5_HUMAN (SEC14-like protein 5 OS=Homo sapiens GN=SEC14L5 PE=2 SV=3)

HSP 1 Score: 109.768 bits (273), Expect = 2.298e-23
Identity = 64/210 (30.48%), Postives = 116/210 (55.24%), Query Frame = 3
Query:   12 QSKDMWAEMLQWRKEFGVDTIMEDFEFKEINEVLSYYPHGYHGVDKEGRPVYIERLGKVDSNKLMQVTTMDRYIRYHVQGFEKAFAVKFPACTIAAKRHIDSSTSILDVQGVGLKNFSKNARELILRLQKIDGDNYPETLHQMFIINAGPGFRLLWNTVKSFLDPKTTSKIHVL-GNKYQSK--LLEIIDARELPEFLGGTLYC-ADQGG 629
            ++++M  + L WRK+  VD +++ ++   + E   +Y  G+H  D +GRP+YI RLG++D+  LM+    +  +R HV    +    +    T    R I S T +LD++G+ +++  +   + +LR+ ++  DNYPETL ++ I+ A   F +LW  +  F++  T  K  +  G+ YQ    L++ +D   +P+FLGG   C   +GG
Sbjct:  280 KAREMLRQSLSWRKQHQVDLLLQTWQPPALLE--EFYAGGWHYQDIDGRPLYILRLGQMDTKGLMKAVGEEALLR-HVLSVNEEGQKRCEGSTRQLGRPISSWTCLLDLEGLNMRHLWRPGVKALLRMIEVVEDNYPETLGRLLIVRAPRVFPVLWTLISPFINENTRRKFLIYSGSNYQGPGGLVDYLDREVIPDFLGGESVCNVPEGG 486          
BLAST of EY692983 vs. ExPASy Swiss-Prot
Match: S14L1_HUMAN (SEC14-like protein 1 OS=Homo sapiens GN=SEC14L1 PE=1 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 3.002e-23
Identity = 66/210 (31.43%), Postives = 116/210 (55.24%), Query Frame = 3
Query:   12 QSKDMWAEMLQWRKEFGVDTIMEDFEFKEINEVLSYYPHGYHGVDKEGRPVYIERLGKVDSNKLMQVTTMDRYIRYHVQGFEKAFAVKFPACTIAAKRHIDSSTSILDVQGVGLKNFSKNARELILRLQKIDGDNYPETLHQMFIINAGPGFRLLWNTVKSFLDPKTTSKIHV-LGNKYQSK--LLEIIDARELPEFLGGTLYC-ADQGG 629
            +++++  + L WRK+  VD I+E +   ++ +   YY  G+H  DK+GRP+Y+ RLG++D+  L++    +  +RY +   E+    +    T    R I S T ++D++G+ +++  +   + +LR+ ++   NYPETL ++ I+ A   F +LW  V  F+D  T  K  +  GN YQ    LL+ ID   +P+FL G   C   +GG
Sbjct:  293 KAREIMCQSLTWRKQHQVDYILETWTPPQVLQ--DYYAGGWHHHDKDGRPLYVLRLGQMDTKGLVRALGEEALLRYVLSVNEERLR-RCEENTKVFGRPISSWTCLVDLEGLNMRHLWRPGVKALLRIIEVVEANYPETLGRLLILRAPRVFPVLWTLVSPFIDDNTRRKFLIYAGNDYQGPGGLLDYIDKEIIPDFLSGECMCEVPEGG 499          
The following BLAST results are available for this feature:
BLAST of EY692983 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 25
Match NameE-valueIdentityDescription
SEC14_SCHPO1.097e-4142.25Sec14 cytosolic factor OS=Schizosaccharomyces pomb... [more]
SEC14_YARLI5.096e-3939.74SEC14 cytosolic factor OS=Yarrowia lipolytica GN=S... [more]
SEC14_KLULA3.652e-3737.66SEC14 cytosolic factor OS=Kluyveromyces lactis GN=... [more]
SEC14_CANAL1.813e-3637.99SEC14 cytosolic factor OS=Candida albicans GN=SEC1... [more]
SEC14_CANGA1.534e-3537.09SEC14 cytosolic factor OS=Candida glabrata GN=SEC1... [more]
SEC14_YEAST7.615e-3535.78SEC14 cytosolic factor OS=Saccharomyces cerevisiae... [more]
SEC14_ASHGO4.179e-3336.62SEC14 cytosolic factor OS=Ashbya gossypii GN=SEC14... [more]
YKJ1_YEAST1.344e-3136.95CRAL-TRIO domain-containing protein YKL091C OS=Sac... [more]
S14L5_HUMAN2.298e-2330.48SEC14-like protein 5 OS=Homo sapiens GN=SEC14L5 PE... [more]
S14L1_HUMAN3.002e-2331.43SEC14-like protein 1 OS=Homo sapiens GN=SEC14L1 PE... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-700-006-E06-CT.F Sweet orange fruit, development stadium (1 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY692983 ID=EY692983; Name=EY692983; organism=Citrus sinensis; type=EST; length=922bp
CCACGCGTCCGCAAAGCAAAGATATGTGGGCCGAGATGCTTCAATGGAGG
AAGGAATTCGGTGTTGACACTATTATGGAGGATTTCGAGTTCAAAGAAAT
AAATGAAGTTTTGAGTTATTACCCCCATGGTTACCATGGTGTGGACAAGG
AGGGAAGACCTGTTTACATTGAAAGATTGGGAAAAGTTGATTCTAACAAA
CTTATGCAAGTTACCACTATGGATCGATATATAAGGTATCATGTTCAAGG
ATTTGAAAAAGCCTTTGCAGTAAAGTTTCCAGCTTGTACAATTGCTGCAA
AGAGGCACATTGATTCGAGCACTTCAATTTTAGATGTTCAAGGCGTGGGC
CTTAAGAATTTTTCCAAGAATGCTCGTGAGCTCATTTTGCGGCTGCAGAA
GATTGATGGTGATAATTATCCTGAGACTCTTCATCAAATGTTTATCATTA
ATGCTGGTCCTGGATTTAGGCTGCTGTGGAACACTGTGAAAAGTTTTCTA
GACCCTAAAACAACTTCCAAGATTCATGTTCTTGGAAACAAGTACCAGAG
CAAATTGCTTGAGATAATTGATGCCAGAGAGTTGCCAGAGTTTCTTGGAG
GTACCCTGTATTGTGCAGATCAGGGAGGTTGTCTTCGGTCTGATAAGGGG
CATGGCAAAACCAGAGATATTGAGATGGTGCTTAATGGGAGAGCACCGCG
AGCAGGCAGATGTGAAAGNTCTAACAGCGATGGGAAAGTCATTGCTTATG
CTAGCCGCNATGCNTTAAAAGGAGGGAACATCTACTGCTGAGTCTGATCA
AAACAAAAAATTTGCTTCCCCAAGGCATGAGAGTTATCCCTCTTCNATTG
ACTCCGGCCGGAAAAGCAAAGTTGTTGGAAACACTATGCTGGACCTCCCG
GGAATAAGAAATGGTCCAGGGT
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