EY711569

Overview
NameEY711569
Unique NameEY711569
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length510
Libraries
Library NameType
Sweet orange fruit, development stadium (3 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY711569 vs. ExPASy Swiss-Prot
Match: METE_THEYD (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Thermodesulfovibrio yellowstonii (strain ATCC 51303 / DSM 11347 / YP87) GN=metE PE=3 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 1.016e-27
Identity = 37/55 (67.27%), Postives = 44/55 (80.00%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGCL 166
            L GFA T NGWVQSYGSRCV+PP+IYGDV R KA+TV  +  AQS+T RP+KG +
Sbjct:  488 LKGFAITKNGWVQSYGSRCVRPPVIYGDVWRDKALTVEETLYAQSLTPRPVKGIM 542

HSP 2 Score: 64.3142 bits (155), Expect = 1.016e-27
Identity = 43/111 (38.74%), Postives = 57/111 (51.35%), Query Frame = 1 
Query:  148 PNEGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGLTVIQL--------MRLP*GKDCSLGI*TCFYLDWAVPSSEYPLGGPEP-TQITPHV-VTQLNEI 450
            P +G++TG VTIL WS+ R D  R E  YQIALA+K+EV +LEK G+ +IQ+        + L   K          Y DW + S         P TQI  H+  +  NEI
Sbjct:  537 PVKGIMTGAVTILQWSYPRKDISRREIAYQIALALKEEVLELEKRGIKIIQIDEPAFREGLPLKRNKQDE-------YFDWTINSFRLTTADVSPFTQIHTHMCYSDFNEI 640
[Close]
BLAST of EY711569 vs. ExPASy Swiss-Prot
Match: METE_LACPL (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Lactobacillus plantarum GN=metE PE=3 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 2.576e-25
Identity = 33/57 (57.89%), Postives = 43/57 (75.44%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGCLLA 172
            L+GF  T NGWVQSYGSR V+PP+I+GDV+  + +TV  S  AQS+T +P+KG L A
Sbjct:  507 LTGFYATQNGWVQSYGSRGVRPPVIFGDVAYTEPITVAESVYAQSLTDQPVKGMLTA 563

HSP 2 Score: 68.5514 bits (166), Expect = 2.576e-25
Identity = 31/52 (59.62%), Postives = 43/52 (82.69%), Query Frame = 1 
Query:  148 PNEGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGLTVIQL 303
            P +GMLT P+TI+NWSFVR+D PR +   QIALA++ EV++LEKAG+ +IQ+
Sbjct:  556 PVKGMLTAPLTIINWSFVRDDIPRAQVQNQIALALRQEVQNLEKAGIKIIQV 607
[Close]
BLAST of EY711569 vs. ExPASy Swiss-Prot
Match: METE_ARATH (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Arabidopsis thaliana GN=CIMS PE=1 SV=1)

HSP 1 Score: 111.694 bits (278), Expect = 1.985e-24
Identity = 53/66 (80.30%), Postives = 58/66 (87.88%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGCLLALSPFSTGPL 199
            LSGFAFTANGWVQSYGSRCVKPP+IYGDVSRPKAMTVFWS++AQSMT+RPMKG L       TGP+
Sbjct:  504 LSGFAFTANGWVQSYGSRCVKPPVIYGDVSRPKAMTVFWSAMAQSMTSRPMKGML-------TGPV 562

HSP 2 Score: 109.383 bits (272), Expect = 1.002e-23
Identity = 64/110 (58.18%), Postives = 73/110 (66.36%), Query Frame = 1 
Query:  142 SSPNEGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGLTVIQLMRLP*GKDCSL-GI*TCFYLDWAVPSSEYP-LGGPEPTQITPHV-VTQLNEIFPSL 462
            S P +GMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEK G+ VIQ+      +   L      FYLDWAV S      G  + TQI  H+  +  N+I  S+
Sbjct:  551 SRPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKGGIGVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDSTQIHTHMCYSHFNDIIHSI 660
[Close]
BLAST of EY711569 vs. ExPASy Swiss-Prot
Match: METE_SOLSC (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Solenostemon scutellarioides GN=MET PE=1 SV=2)

HSP 1 Score: 109.768 bits (273), Expect = 7.670e-24
Identity = 64/108 (59.26%), Postives = 74/108 (68.52%), Query Frame = 1 
Query:  148 PNEGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGLTVIQLMRLP*GKDCSL-GI*TCFYLDWAVPSSEYP-LGGPEPTQITPHV-VTQLNEIFPSL 462
            P +GMLTGPVTILNWSFVRNDQPR ETCYQIALAIKDEVEDLEKAG+TVIQ+      +   L      FYLDWAV S     +G  + TQI  H+  +  N+I  S+
Sbjct:  552 PMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGITVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNVGVQDTTQIHTHMCYSNFNDIIHSI 659

HSP 2 Score: 108.227 bits (269), Expect = 2.195e-23
Identity = 53/66 (80.30%), Postives = 55/66 (83.33%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGCLLALSPFSTGPL 199
            LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPK MTVFWS+ AQSMT RPMKG L       TGP+
Sbjct:  503 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKPMTVFWSTAAQSMTQRPMKGML-------TGPV 561
[Close]
BLAST of EY711569 vs. ExPASy Swiss-Prot
Match: METE_LISW6 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Listeria welshimeri serovar 6b (strain ATCC 35897 / DSM 20650 / SLCC5334) GN=metE PE=3 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 1.029e-23
Identity = 34/57 (59.65%), Postives = 42/57 (73.68%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGCLLA 172
            L+GF  T  GWVQSYGSR V+PP+IYGDV+  + +TV  S  AQS+T RP+KG L A
Sbjct:  504 LAGFQATKFGWVQSYGSRAVRPPLIYGDVAFTEEITVKESVYAQSLTKRPVKGMLTA 560

HSP 2 Score: 62.7734 bits (151), Expect = 1.029e-23
Identity = 29/52 (55.77%), Postives = 38/52 (73.08%), Query Frame = 1 
Query:  148 PNEGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGLTVIQL 303
            P +GMLT PVTI+NWSFVR+D P      Q+ LA++ EVE LE+ G+ VIQ+
Sbjct:  553 PVKGMLTAPVTIINWSFVRDDVPESVVANQVGLALRKEVEALERNGIKVIQV 604
[Close]
BLAST of EY711569 vs. ExPASy Swiss-Prot
Match: METE_LISMO (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Listeria monocytogenes GN=metE PE=3 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 1.029e-23
Identity = 34/57 (59.65%), Postives = 42/57 (73.68%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGCLLA 172
            L+GF  T  GWVQSYGSR V+PP+IYGDV+  + +TV  S  AQS+T RP+KG L A
Sbjct:  504 LAGFQATKFGWVQSYGSRAVRPPLIYGDVAFTEEITVKESVYAQSLTKRPVKGMLTA 560

HSP 2 Score: 62.7734 bits (151), Expect = 1.029e-23
Identity = 29/52 (55.77%), Postives = 38/52 (73.08%), Query Frame = 1 
Query:  148 PNEGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGLTVIQL 303
            P +GMLT PVTI+NWSFVR+D P      Q+ LA++ EVE LE+ G+ VIQ+
Sbjct:  553 PVKGMLTAPVTIINWSFVRDDVPESVVANQVGLALRKEVEALERNGIKVIQV 604
[Close]
BLAST of EY711569 vs. ExPASy Swiss-Prot
Match: METE_LISMF (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Listeria monocytogenes serotype 4b (strain F2365) GN=metE PE=3 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 1.029e-23
Identity = 34/57 (59.65%), Postives = 42/57 (73.68%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGCLLA 172
            L+GF  T  GWVQSYGSR V+PP+IYGDV+  + +TV  S  AQS+T RP+KG L A
Sbjct:  504 LAGFQATKFGWVQSYGSRAVRPPLIYGDVAFTEEITVKESVYAQSLTKRPVKGMLTA 560

HSP 2 Score: 62.7734 bits (151), Expect = 1.029e-23
Identity = 29/52 (55.77%), Postives = 38/52 (73.08%), Query Frame = 1 
Query:  148 PNEGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGLTVIQL 303
            P +GMLT PVTI+NWSFVR+D P      Q+ LA++ EVE LE+ G+ VIQ+
Sbjct:  553 PVKGMLTAPVTIINWSFVRDDVPESVVANQVGLALRKEVEALERNGIKVIQV 604
[Close]
BLAST of EY711569 vs. ExPASy Swiss-Prot
Match: METE_LISMC (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Listeria monocytogenes serotype 4b (strain Clip81459) GN=metE PE=3 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 1.029e-23
Identity = 34/57 (59.65%), Postives = 42/57 (73.68%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGCLLA 172
            L+GF  T  GWVQSYGSR V+PP+IYGDV+  + +TV  S  AQS+T RP+KG L A
Sbjct:  504 LAGFQATKFGWVQSYGSRAVRPPLIYGDVAFTEEITVKESVYAQSLTKRPVKGMLTA 560

HSP 2 Score: 62.7734 bits (151), Expect = 1.029e-23
Identity = 29/52 (55.77%), Postives = 38/52 (73.08%), Query Frame = 1 
Query:  148 PNEGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGLTVIQL 303
            P +GMLT PVTI+NWSFVR+D P      Q+ LA++ EVE LE+ G+ VIQ+
Sbjct:  553 PVKGMLTAPVTIINWSFVRDDVPESVVANQVGLALRKEVEALERNGIKVIQV 604
[Close]
BLAST of EY711569 vs. ExPASy Swiss-Prot
Match: METE_LISIN (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Listeria innocua GN=metE PE=3 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 1.029e-23
Identity = 34/57 (59.65%), Postives = 42/57 (73.68%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGCLLA 172
            L+GF  T  GWVQSYGSR V+PP+IYGDV+  + +TV  S  AQS+T RP+KG L A
Sbjct:  504 LAGFQATKFGWVQSYGSRAVRPPLIYGDVAFTEEITVKESVYAQSLTKRPVKGMLTA 560

HSP 2 Score: 62.7734 bits (151), Expect = 1.029e-23
Identity = 29/52 (55.77%), Postives = 38/52 (73.08%), Query Frame = 1 
Query:  148 PNEGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGLTVIQL 303
            P +GMLT PVTI+NWSFVR+D P      Q+ LA++ EVE LE+ G+ VIQ+
Sbjct:  553 PVKGMLTAPVTIINWSFVRDDVPESVVANQVGLALRKEVEALERNGIKVIQV 604
[Close]
BLAST of EY711569 vs. ExPASy Swiss-Prot
Match: METE_MESCR (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Mesembryanthemum crystallinum GN=METE PE=2 SV=1)

HSP 1 Score: 108.997 bits (271), Expect = 1.287e-23
Identity = 53/66 (80.30%), Postives = 56/66 (84.85%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGCLLALSPFSTGPL 199
            LSGFAF+ANGWVQSYGSRCVKPPIIYGDVSRP  MTVFWSS+AQSMTARPMKG L       TGP+
Sbjct:  504 LSGFAFSANGWVQSYGSRCVKPPIIYGDVSRPNPMTVFWSSMAQSMTARPMKGML-------TGPV 562

HSP 2 Score: 105.531 bits (262), Expect = 1.447e-22
Identity = 62/108 (57.41%), Postives = 73/108 (67.59%), Query Frame = 1 
Query:  148 PNEGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGLTVIQLMRLP*GKDCSL-GI*TCFYLDWAVPSSEYP-LGGPEPTQITPHV-VTQLNEIFPSL 462
            P +GMLTGPVTILNWSFVRNDQPRHETCYQIALAIK+EVEDLEKAG+ VIQ+      +   L      FYL WAV S     +G  + TQI  H+  +  N+I  S+
Sbjct:  553 PMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKNEVEDLEKAGINVIQIDEAALREGLPLRKSEHDFYLKWAVHSFRITNVGVQDTTQIHTHMCYSNFNDIIHSI 660
The following BLAST results are available for this feature:
BLAST of EY711569 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 268
Match NameE-valueIdentityDescription
METE_THEYD1.016e-2767.275-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_LACPL2.576e-2557.895-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_ARATH1.985e-2480.305-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_SOLSC7.670e-2459.265-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_LISW61.029e-2359.655-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_LISMO1.029e-2359.655-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_LISMF1.029e-2359.655-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_LISMC1.029e-2359.655-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_LISIN1.029e-2359.655-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_MESCR1.287e-2380.305-methyltetrahydropteroyltriglutamate--homocystein... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-702-009-C02-CT.F Sweet orange fruit, development stadium (3 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY711569 ID=EY711569; Name=EY711569; organism=Citrus sinensis; type=EST; length=510bp
ATTATCAGGTTTCGCCTTCACTGCCAATGGGTGGGTGCAATCCTATGGAT
CTCGCTGTGTGAAGCCACCCATCATCTATGGTGATGTGAGCCGTCCCAAG
GCTATGACCGTCTTCTGGTCCTCCTTGGCTCAGAGCATGACAGCTCGCCC
AATGAAGGGATGCTTACTGGCCCTGTCACCATTCTCAACTGGTCCTTTGT
TAGAAATGACCAGCCCAGACATGAAACCTGCTACCAGATTGCTTTGGCCA
TCAAGGACGAAGTCGAAGATCTTGAAAAGGCAGGCTTAACGGTAATCCAA
TTGATGAGGCTGCCCTGAGGAAAGGATTGCTCCTTAGGAATCTGAACATG
CTTCTACTTGGACTGGGCAGTCCCCTCCTCAGAATACCCACTGGGGGGTC
CAGAACCTACCCAAATCACCCCCCATGTGGTCACCCAACTCAACGAAATT
TTCCCCTCATTATCCACATGGATGTGGAGGGGACCCCTTGAAAACCTCCC
TTTGATAAAA
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