EY711448

Overview
NameEY711448
Unique NameEY711448
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length882
Libraries
Library NameType
Sweet orange fruit, development stadium (3 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY711448 vs. ExPASy Swiss-Prot
Match: PGKY_TOBAC (Phosphoglycerate kinase, cytosolic OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 229.18 bits (583), Expect = 2.458e-59
Identity = 133/209 (63.64%), Postives = 147/209 (70.33%), Query Frame = 1 
Query:   22 IRVIESLFFTPDILLLGGGMIFTFYKAQGHSVGSSLVEEDKLDLGTSLMEKAKSKGVSLLLPTDVVIADKFAADANSKVVPATAIPDGWMGLDVAPDAIKSFTEALDTTKTIIWNGPMGVLEF**ICCWH*GNCQDSCGTHRDGSDHNHWMRRLS--CIRTYTWSG*QHEPIS-----------STGGGASLKLLDGKSLPGVLALDDA 609
            I VIESL    D+LLLGGGMIFTFYKAQG++VGSSLVEEDKLDL TSLMEKAK+KGVSLLLPTDVVIADKFAADANSKVVPA+ IPDGWMGLD+ PDAIKSF  ALDTTKT+IWNGPMGV EF           + + GT          +  LS   + T    G     +            STGGGASL+LL+GK LPGVLALDDA
Sbjct:  207 IGVIESLLEKVDVLLLGGGMIFTFYKAQGYAVGSSLVEEDKLDLATSLMEKAKAKGVSLLLPTDVVIADKFAADANSKVVPASEIPDGWMGLDIGPDAIKSFGSALDTTKTVIWNGPMGVFEF----------DKFAAGTEAIAKK----LAELSGKGVTTIIGGGDSVAAVEKVGLAEKMSHISTGGGASLELLEGKPLPGVLALDDA 401
[Close]
BLAST of EY711448 vs. ExPASy Swiss-Prot
Match: PGKH_TOBAC (Phosphoglycerate kinase, chloroplastic OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 221.09 bits (562), Expect = 6.695e-57
Identity = 126/206 (61.17%), Postives = 146/206 (70.87%), Query Frame = 1 
Query:   22 IRVIESLFFTPDILLLGGGMIFTFYKAQGHSVGSSLVEEDKLDLGTSLMEKAKSKGVSLLLPTDVVIADKFAADANSKVVPATAIPDGWMGLDVAPDAIKSFTEALDTTKTIIWNGPMGVLEF**ICCWH*G---NCQDSCG----THRDGSDHNHWMRRLSCIRTYTWSG*QHEPISSTGGGASLKLLDGKSLPGVLALDDA*TP 618
            I VIESL    DILLLGGGMIFTFYKAQG SVGSSLVEEDKL+L TSL+EKAK+KGVSLLLP+DVVIADKFA DANSK+VPA+AIPDGWMGLD+ PD++K+F +ALDTTKT+IWNGPMGV EF               D  G    T   G D    + ++      +          STGGGASL+LL+GK LPGV+ALD+A  P
Sbjct:  281 IGVIESLLEKCDILLLGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLEKAKAKGVSLLLPSDVVIADKFAPDANSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTKTVIWNGPMGVFEFDKFAVGTEAIAKKLADLSGKGVTTIIGGGDSVAAVEKVGVASVMSH--------ISTGGGASLELLEGKVLPGVIALDEADAP 478
[Close]
BLAST of EY711448 vs. ExPASy Swiss-Prot
Match: PGKH_SPIOL (Phosphoglycerate kinase, chloroplastic (Fragment) OS=Spinacia oleracea PE=2 SV=2)

HSP 1 Score: 219.55 bits (558), Expect = 1.948e-56
Identity = 124/203 (61.08%), Postives = 143/203 (70.44%), Query Frame = 1 
Query:   22 IRVIESLFFTPDILLLGGGMIFTFYKAQGHSVGSSLVEEDKLDLGTSLMEKAKSKGVSLLLPTDVVIADKFAADANSKVVPATAIPDGWMGLDVAPDAIKSFTEALDTTKTIIWNGPMGVLEF**ICCWH*GNC-------QDSCGTHRDGSDHNHWMRRLSCIRTYTWSG*QHEPISSTGGGASLKLLDGKSLPGVLALDDA 609
            I VIESL    DILLLGGGMIFTFYKAQG SVGSSLVEEDKLDL TSL+ KAK KGVSLLLPTDVVIADKFAADA+SK+VPA+ IPDGWMGLD+ PD+IK+F+EALDTT+T+IWNGPMGV EF                  +    T   G D    + ++      +          STGGGASL+LL+GK LPGVLAL++A
Sbjct:  234 IGVIESLLEKCDILLLGGGMIFTFYKAQGMSVGSSLVEEDKLDLATSLLAKAKEKGVSLLLPTDVVIADKFAADADSKIVPASGIPDGWMGLDIGPDSIKTFSEALDTTQTVIWNGPMGVFEFEKFAAGTEAIAKKLEEISKKGATTIIGGGDSVAAVEKVGVAEAMSH--------ISTGGGASLELLEGKQLPGVLALNEA 428
[Close]
BLAST of EY711448 vs. ExPASy Swiss-Prot
Match: PGKY_WHEAT (Phosphoglycerate kinase, cytosolic OS=Triticum aestivum PE=2 SV=1)

HSP 1 Score: 218.779 bits (556), Expect = 3.323e-56
Identity = 124/207 (59.90%), Postives = 139/207 (67.15%), Query Frame = 1 
Query:   22 IRVIESLFFTPDILLLGGGMIFTFYKAQGHSVGSSLVEEDKLDLGTSLMEKAKSKGVSLLLPTDVVIADKFAADANSKVVPATAIPDGWMGLDVAPDAIKSFTEALDTTKTIIWNGPMGVLEF**ICCWH*GNCQDSCGTHRDGSDHNHWMRRLSCIRTYTWSG*QHEPIS-----------STGGGASLKLLDGKSLPGVLALDDA 609
            I VIESL    DIL+LGGGMIFTFYKAQG +VG SLVEEDKL+L TSL+E AKSKGV LLLPTDVV+ADKFAADA SK+VPATAIPDGWMGLDV PD+IK+F EALDTTKT+IWNGPMGV EF            +      D             + T    G     +            STGGGASL+LL+GK LPGVLALD+A
Sbjct:  207 IGVIESLLAKVDILILGGGMIFTFYKAQGLAVGKSLVEEDKLELATSLIETAKSKGVKLLLPTDVVVADKFAADAESKIVPATAIPDGWMGLDVGPDSIKTFAEALDTTKTVIWNGPMGVFEF------------EKFAAGTDAIAKQLAELTGKGVTTIIGGGDSVAAVEKAGLADKMSHISTGGGASLELLEGKPLPGVLALDEA 401
[Close]
BLAST of EY711448 vs. ExPASy Swiss-Prot
Match: PGKH_WHEAT (Phosphoglycerate kinase, chloroplastic OS=Triticum aestivum PE=2 SV=1)

HSP 1 Score: 212.616 bits (540), Expect = 2.381e-54
Identity = 120/206 (58.25%), Postives = 143/206 (69.42%), Query Frame = 1 
Query:   22 IRVIESLFFTPDILLLGGGMIFTFYKAQGHSVGSSLVEEDKLDLGTSLMEKAKSKGVSLLLPTDVVIADKFAADANSKVVPATAIPDGWMGLDVAPDAIKSFTEALDTTKTIIWNGPMGVLEF**ICCWH*GNCQDSCGTHRDGSDHNHWMRRLSCIRTYTWSG*QHEPIS-----------STGGGASLKLLDGKSLPGVLALDD 606
            I VIESL    DILLLGGGMIFTFYKAQG SVGSSLVEEDKL+L TSL+ KAK+KGVSLLLP+DV+IADKFA DANS+ VPA+AIPDGWMGLD+ PD++K+F +ALDTT+TIIWNGPMGV EF           + + GT           ++   + T    G     +            STGGGASL+LL+GK LPGV+ALD+
Sbjct:  278 IGVIESLLEKCDILLLGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLAKAKAKGVSLLLPSDVIIADKFAPDANSQTVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEF----------DKFAVGTESIAKKLAELSKK--GVTTIIGGGDSVAAVEKVGVADVMSHISTGGGASLELLEGKELPGVVALDE 471
[Close]
BLAST of EY711448 vs. ExPASy Swiss-Prot
Match: PGKH_ARATH (Phosphoglycerate kinase, chloroplastic OS=Arabidopsis thaliana GN=At1g56190 PE=1 SV=2)

HSP 1 Score: 210.305 bits (534), Expect = 1.182e-53
Identity = 120/207 (57.97%), Postives = 141/207 (68.12%), Query Frame = 1 
Query:   22 IRVIESLFFTPDILLLGGGMIFTFYKAQGHSVGSSLVEEDKLDLGTSLMEKAKSKGVSLLLPTDVVIADKFAADANSKVVPATAIPDGWMGLDVAPDAIKSFTEALDTTKTIIWNGPMGVLEF**ICCWH*GNCQDSCGTHRDGSDHNHWMRRLSCIRTYTWSG*QHEPIS-----------STGGGASLKLLDGKSLPGVLALDDA 609
            I VIESL    DILLLGGGMIFTFYKAQG SVGSSLVEEDKL+L T L+ KAK+KGVSLLLPTDVV+ADKFA DANSK+VPA+ I DGWMGLD+ PD+IK+F EALDTT+T+IWNGPMGV E            + + GT    +       +   + T    G     +            STGGGASL+LL+GK LPGV+ALD+A
Sbjct:  279 IGVIESLLEKCDILLLGGGMIFTFYKAQGLSVGSSLVEEDKLELATELLAKAKAKGVSLLLPTDVVVADKFAPDANSKIVPASGIEDGWMGLDIGPDSIKTFNEALDTTQTVIWNGPMGVFEM----------EKFAAGTEAIANKLAELSEK--GVTTIIGGGDSVAAVEKVGVAGVMSHISTGGGASLELLEGKVLPGVIALDEA 473
[Close]
BLAST of EY711448 vs. ExPASy Swiss-Prot
Match: PGK_SYNE7 (Phosphoglycerate kinase OS=Synechococcus elongatus (strain PCC 7942) GN=pgk PE=3 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 3.330e-48
Identity = 111/197 (56.35%), Postives = 129/197 (65.48%), Query Frame = 1 
Query:   22 IRVIESLFFTPDILLLGGGMIFTFYKAQGHSVGSSLVEEDKLDLGTSLMEKAKSKGVSLLLPTDVVIADKFAADANSKVVPATAIPDGWMGLDVAPDAIKSFTEALDTTKTIIWNGPMGVLEF**ICCWH*GNCQDSCGTHRDGSDHNHWMRRLSCIRTYTWSG*QHEPIS-STGGGASLKLLDGKSLPGVLALDDA 609
            I VIE+L    D LL+GGGMIFTFYKAQG SVG SLVEEDKLDL  SLM KA+ KGV LLLP DVV+ADKFA DAN+K V   AIPDGWMGLD+ P+++K F EAL   +++IWNGPMGV EF           +   G  R G+     +     +      G   E    STGGGASL+LL+GK LPGV ALDDA
Sbjct:  207 IGVIETLLDKCDKLLIGGGMIFTFYKAQGLSVGGSLVEEDKLDLARSLMAKAQEKGVQLLLPVDVVVADKFAPDANAKTVAIDAIPDGWMGLDIGPESVKQFEEALADCRSVIWNGPMGVFEFDQFAVGTEAIARSLAGLTRKGA--TTIIGGGDSVAAVEKVGVASEMSHISTGGGASLELLEGKVLPGVAALDDA 401
[Close]
BLAST of EY711448 vs. ExPASy Swiss-Prot
Match: PGK_CYAP4 (Phosphoglycerate kinase OS=Cyanothece sp. (strain PCC 7425 / ATCC 29141) GN=pgk PE=3 SV=1)

HSP 1 Score: 191.045 bits (484), Expect = 7.419e-48
Identity = 108/203 (53.20%), Postives = 131/203 (64.53%), Query Frame = 1 
Query:   22 IRVIESLFFTPDILLLGGGMIFTFYKAQGHSVGSSLVEEDKLDLGTSLMEKAKSKGVSLLLPTDVVIADKFAADANSKVVPATAIPDGWMGLDVAPDAIKSFTEALDTTKTIIWNGPMGVLEF**ICCWH*GNCQDSCG-------THRDGSDHNHWMRRLSCIRTYTWSG*QHEPISSTGGGASLKLLDGKSLPGVLALDDA 609
            I VIE+L    D LLLGGGMIFTFYKA+G +VG SLVEEDKL+L  +L  KAK +GV+LLLPTDVV+AD FAADAN++ V   +IPDGWMGLD+ PDA+K+F EAL   KT+IWNGPMGV EF               G       T   G D    + ++      +          STGGGASL+LL+GK LPG++ALDDA
Sbjct:  212 IGVIETLLEKVDKLLLGGGMIFTFYKARGLNVGKSLVEEDKLELARTLEAKAKERGVALLLPTDVVVADAFAADANAQTVSVESIPDGWMGLDIGPDAVKTFQEALSDCKTVIWNGPMGVFEFDKFAVGTEAIAHSLAGLTGKGASTIIGGGDSVAAVEKVGVAEQMSH--------ISTGGGASLELLEGKELPGIVALDDA 406
[Close]
BLAST of EY711448 vs. ExPASy Swiss-Prot
Match: PGK_SYNP6 (Phosphoglycerate kinase OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) GN=pgk PE=3 SV=1)

HSP 1 Score: 189.504 bits (480), Expect = 2.159e-47
Identity = 110/197 (55.84%), Postives = 128/197 (64.97%), Query Frame = 1 
Query:   22 IRVIESLFFTPDILLLGGGMIFTFYKAQGHSVGSSLVEEDKLDLGTSLMEKAKSKGVSLLLPTDVVIADKFAADANSKVVPATAIPDGWMGLDVAPDAIKSFTEALDTTKTIIWNGPMGVLEF**ICCWH*GNCQDSCGTHRDGSDHNHWMRRLSCIRTYTWSG*QHEPIS-STGGGASLKLLDGKSLPGVLALDDA 609
            I VIE+L    D LL+GGGMIFTFYKAQG SVG SLVEEDKLDL  SLM KA+ KGV LLLP DVV+ADKFA DAN+K V   AIPDGWMGLD+ P+++K F  AL   +++IWNGPMGV EF           +   G  R G+     +     +      G   E    STGGGASL+LL+GK LPGV ALDDA
Sbjct:  207 IGVIETLLDKCDKLLIGGGMIFTFYKAQGLSVGGSLVEEDKLDLARSLMAKAQEKGVQLLLPVDVVVADKFAPDANAKTVAIDAIPDGWMGLDIGPESVKQFEGALADCRSVIWNGPMGVFEFDQFAVGTEAIARSLAGLTRKGA--TTIIGGGDSVAAVEKVGVASEMSHISTGGGASLELLEGKVLPGVAALDDA 401
[Close]
BLAST of EY711448 vs. ExPASy Swiss-Prot
Match: PGK_NOSP7 (Phosphoglycerate kinase OS=Nostoc punctiforme (strain ATCC 29133 / PCC 73102) GN=pgk PE=3 SV=1)

HSP 1 Score: 187.193 bits (474), Expect = 1.071e-46
Identity = 110/209 (52.63%), Postives = 131/209 (62.68%), Query Frame = 1 
Query:   22 IRVIESLFFTPDILLLGGGMIFTFYKAQGHSVGSSLVEEDKLDLGTSLMEKAKSKGVSLLLPTDVVIADKFAADANSKVVPATAIPDGWMGLDVAPDAIKSFTEALDTTKTIIWNGPMGVLEF**ICCWH*GNCQDSCGTHRDGSDHNHWMRRLSCIRTYTWSG*QHEPIS-------------STGGGASLKLLDGKSLPGVLALDDA 609
            I VIE+L    D L++GGGMIFTFYKA+G SVG SLVEEDKL+L  SL  KAK +GV+LLLPTDVV+AD FA DANS+ V    IPDGWMGLD+ PD++K F EAL  TKT+IWNGPMGV EF           + + GT        H +  +    T T  G      +             STGGGASL+LL+GK LPG+ ALDDA
Sbjct:  206 IGVIETLLEKCDKLIIGGGMIFTFYKARGLSVGKSLVEEDKLELAKSLEAKAKERGVALLLPTDVVLADNFAPDANSQTVSIENIPDGWMGLDIGPDSVKFFQEALADTKTVIWNGPMGVFEF----------DKFAAGTEAIA----HTLAEIGKTGTTTIIGGGDSVAAVEKVGLADQMSHISTGGGASLELLEGKVLPGIAALDDA 400
The following BLAST results are available for this feature:
BLAST of EY711448 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
PGKY_TOBAC2.458e-5963.64Phosphoglycerate kinase, cytosolic OS=Nicotiana ta... [more]
PGKH_TOBAC6.695e-5761.17Phosphoglycerate kinase, chloroplastic OS=Nicotian... [more]
PGKH_SPIOL1.948e-5661.08Phosphoglycerate kinase, chloroplastic (Fragment) ... [more]
PGKY_WHEAT3.323e-5659.90Phosphoglycerate kinase, cytosolic OS=Triticum aes... [more]
PGKH_WHEAT2.381e-5458.25Phosphoglycerate kinase, chloroplastic OS=Triticum... [more]
PGKH_ARATH1.182e-5357.97Phosphoglycerate kinase, chloroplastic OS=Arabidop... [more]
PGK_SYNE73.330e-4856.35Phosphoglycerate kinase OS=Synechococcus elongatus... [more]
PGK_CYAP47.419e-4853.20Phosphoglycerate kinase OS=Cyanothece sp. (strain ... [more]
PGK_SYNP62.159e-4755.84Phosphoglycerate kinase OS=Synechococcus sp. (stra... [more]
PGK_NOSP71.071e-4652.63Phosphoglycerate kinase OS=Nostoc punctiforme (str... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-702-007-G11-CT.F Sweet orange fruit, development stadium (3 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY711448 ID=EY711448; Name=EY711448; organism=Citrus sinensis; type=EST; length=882bp
CCTTTTTTTTTTTTACTATATATCCGTGTTATTGAATCACTTTTTTTTAC
CCCTGACATTCTATTGCTCGGTGGAGGAATGATCTTTACCTTTTACAAGG
CCCAAGGACACTCTGTTGGTTCATCCCTTGTTGAGGAAGACAAGCTTGAT
CTCGGTACGTCACTTATGGAGAAGGCCAAATCTAAAGGGGTGTCTCTTCT
GCTCCCTACTGATGTTGTTATTGCTGACAAGTTTGCTGCTGATGCCAACA
GCAAGGTGGTGCCAGCAACGGCAATTCCAGATGGTTGGATGGGCTTGGAT
GTTGCACCTGATGCCATCAAGTCATTCACTGAAGCTTTGGATACTACTAA
AACCATCATCTGGAATGGACCTATGGGTGTGCTTGAATTTTGATAAATTT
GCTGCTGGCACTGAGGCAATTGCCAAGACTCTTGCGGAACTCACCGGGAC
GGGAGTGACCACAATCATTGGATGAGGCGACTCAGTTGCATCCGTACATA
CACGTGGTCTGGCTGACAACATGAGCCTATATCCTCCACTGGAGGTGGAG
CCAGCTTAAAGCTCCTTGACGGCAAATCCCTCCCTGGCGTGCTCGCTCTT
GATGATGCTTAAACCCCCTTCCTGTACTTTCCTTTTTTAAATGTGCCCTT
TCCACATTTGATTGTGCGGTCGGATGCCACCCCAAGTGGATCAAAGCTAA
ACTCCTCCTAAACCTTGGCTCTGAAACGCTGTAACATAAAAATAACTCTT
TTCCCAAGGAACAATGGTGAGCTATTTTCTGGGTAGAATCCCTAATTGAT
ATTTCGGTCGTAAGGCTCTTACCTTCCTGACAAAAAACACAATCAACTCA
AATAAGCGCGGCCTCCTTAATACCCATCTTAC
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