EY711354 | Citrus Genome Database

Overview


Name	EY711354
Unique Name	EY711354
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	918

Libraries


Library Name	Type
Sweet orange fruit, development stadium (3 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
EY711354_ssr152	microsatellite	EY711354_ssr152:152..166.	BLAST: Citrus ESTs to Prunus persica proteins V1

Homology

[Close]

BLAST of EY711354 vs. ExPASy Swiss-Prot
Match: ARL2_DROME (ADP-ribosylation factor-like protein 2 OS=Drosophila melanogaster GN=Arf84F PE=2 SV=2)

HSP 1 Score: 91.6633 bits (226), Expect = 2.367e-38
Identity = 40/58 (68.97%), Postives = 48/58 (82.76%), Query Frame = 2

Query:  401 EFDRYTLNIWDVGGQRTIRSYWRNYFEQTDGLVWVVDSSDLRRLDDCKMELDNLLKEE 574
            E + YTLN+WDVGGQ+++RSYWRNYFE TDGLVWVVDS+D  RL+ C  EL  LL+EE
Sbjct:   56 EHNGYTLNMWDVGGQKSLRSYWRNYFESTDGLVWVVDSADRMRLESCGQELQVLLQEE 113

HSP 2 Score: 56.9954 bits (136), Expect = 2.367e-38
Identity = 29/69 (42.03%), Postives = 43/69 (62.32%), Query Frame = 1

Query:  589 MFQCQKLSGASLLILXNKQDINGALTPTEIAKVLDXGAMDKTRHWKIVGCSAYTSEGLLXGFDXXVXDM 795
            + Q ++L+GA+LL+L NKQD+ GAL+  EI ++L    +  T HW + G SA T E LL   D  + D+
Sbjct:  109 LLQEERLAGATLLVLCNKQDLPGALSSNEIKEILHLEDI-TTHHWLVAGVSAVTGEKLLSSMDWLIADI 176

HSP 3 Score: 51.9878 bits (123), Expect = 2.367e-38
Identity = 23/35 (65.71%), Postives = 28/35 (80.00%), Query Frame = 1

Query:  175 GLDNSGKTTIVLKINGEDTSVISPTLGFNIKTITY 279
            GLDN+GKTTI+ + NGE    ISPTLGFNIKT+ +
Sbjct:   23 GLDNAGKTTILKRFNGEPIDTISPTLGFNIKTLEH 57

HSP 4 Score: 20.7866 bits (42), Expect = 2.367e-38
Identity = 9/21 (42.86%), Postives = 16/21 (76.19%), Query Frame = 3

Query:   15 VRPRVLKKEKEMRILMVYVIN 77
            V  ++ +KE+EMRIL++ + N
Sbjct:    6 VLKKMRQKEREMRILLLGLDN 26

[Close]

BLAST of EY711354 vs. ExPASy Swiss-Prot
Match: ARL2_CAEBR (ADP-ribosylation factor-like protein 2 OS=Caenorhabditis briggsae GN=evl-20.1 PE=3 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 1.339e-34
Identity = 36/57 (63.16%), Postives = 44/57 (77.19%), Query Frame = 2

Query:  404 FDRYTLNIWDVGGQRTIRSYWRNYFEQTDGLVWVVDSSDLRRLDDCKMELDNLLKEE 574
            F  + LN+WDVGGQ+++RSYW+NYFE TD L+WVVDSSD  RL  C  EL  LL+EE
Sbjct:   57 FKDFQLNLWDVGGQKSLRSYWKNYFESTDALIWVVDSSDRERLTQCSEELKKLLQEE 113

HSP 2 Score: 56.9954 bits (136), Expect = 1.339e-34
Identity = 30/72 (41.67%), Postives = 43/72 (59.72%), Query Frame = 1

Query:  580 LTNMFQCQKLSGASLLILXNKQDINGALTPTEIAKVLDXGAMDKTRHWKIVGCSAYTSEGLLXGFDXXVXDM 795
            L  + Q ++LSGASLL+L NK D+ GA+    IA+VL+  ++ KT HWKI  C A + E L+        D+
Sbjct:  106 LKKLLQEERLSGASLLVLANKSDLPGAIDVNSIAQVLELQSI-KTHHWKIFSCCALSGERLVQAMTWLCDDV 176

HSP 3 Score: 46.2098 bits (108), Expect = 1.339e-34
Identity = 21/40 (52.50%), Postives = 29/40 (72.50%), Query Frame = 1

Query:  175 GLDNSGKTTIVLKINGEDTSVISPTLGFNIKTITYQK*EL 294
            GLDN+GKTT++ K   E T  I PTLGF+IKT+ ++  +L
Sbjct:   23 GLDNAGKTTLMKKFLDEPTDTIEPTLGFDIKTVHFKDFQL 62

[Close]

BLAST of EY711354 vs. ExPASy Swiss-Prot
Match: ARF5_ARATH (Probable ADP-ribosylation factor At2g18390 OS=Arabidopsis thaliana GN=At2g18390 PE=2 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 1.341e-34
Identity = 96/207 (46.38%), Postives = 113/207 (54.59%), Query Frame = 1

Query:  175 GLDNSGKTTIVLKINGEDTSVISPTLGFNIKTITYQK*ELFIP*FRIILCQPKMRI*SL*TLFSF*REHFSSDIF*I*QVHFKHMGCRRPKDYKIILEELL*AN*WFGVGG**FRS*KTG*LQNGTRQSFKGRGSLTNMFQCQKLSGASLLILXNKQDINGALTPTEIAKVLDXGAMDKTRHWKIVGCSAYTSEGLLXGFDXXVXDM 795
            GLDNSGKTTIVLKINGEDTSVISPTLGFNIKTI YQK  L I             +    T+ S+ R +F      +  V    +  RR  D K+ L+ LL                                       + ++L+G+SLLIL NKQDI GALTP EI KVL+  +MDK+RHWKIVGCSAYT EGLL GFD  V D+
Sbjct:   23 GLDNSGKTTIVLKINGEDTSVISPTLGFNIKTIIYQKYTLNI-----------WDVGGQKTIRSYWRNYFEQTDGLVWVVDSSDL--RRLDDCKMELDNLL---------------------------------------KEERLAGSSLLILANKQDIQGALTPDEIGKVLNLESMDKSRHWKIVGCSAYTGEGLLEGFDWLVQDI 177

HSP 2 Score: 26.1794 bits (56), Expect = 1.341e-34
Identity = 12/18 (66.67%), Postives = 15/18 (83.33%), Query Frame = 3

Query:   24 RVLKKEKEMRILMVYVIN 77
            ++ KKEKEMRILMV + N
Sbjct:    9 KIKKKEKEMRILMVGLDN 26

[Close]

BLAST of EY711354 vs. ExPASy Swiss-Prot
Match: ARL2_CAEEL (ADP-ribosylation factor-like protein 2 OS=Caenorhabditis elegans GN=evl-20 PE=2 SV=2)

HSP 1 Score: 83.1889 bits (204), Expect = 1.490e-32
Identity = 36/57 (63.16%), Postives = 43/57 (75.44%), Query Frame = 2

Query:  404 FDRYTLNIWDVGGQRTIRSYWRNYFEQTDGLVWVVDSSDLRRLDDCKMELDNLLKEE 574
            F  + LN+WDVGGQ+++RSYW+NYFE TD L+WVVDSSD  RL  C  EL  LL EE
Sbjct:   57 FKDFQLNLWDVGGQKSLRSYWKNYFESTDALIWVVDSSDRERLLQCSEELKKLLGEE 113

HSP 2 Score: 52.373 bits (124), Expect = 1.490e-32
Identity = 26/65 (40.00%), Postives = 40/65 (61.54%), Query Frame = 1

Query:  601 QKLSGASLLILXNKQDINGALTPTEIAKVLDXGAMDKTRHWKIVGCSAYTSEGLLXGFDXXVXDM 795
            ++L+GASLL+L NK D+ GA+    IA+VLD  ++ K+ HWKI  C A + + L+        D+
Sbjct:  113 ERLAGASLLVLANKSDLPGAIDVNSIAQVLDLHSI-KSHHWKIFSCCALSGDRLVQAMTWLCDDV 176

HSP 3 Score: 46.2098 bits (108), Expect = 1.490e-32
Identity = 21/40 (52.50%), Postives = 29/40 (72.50%), Query Frame = 1

Query:  175 GLDNSGKTTIVLKINGEDTSVISPTLGFNIKTITYQK*EL 294
            GLDN+GKTT++ K   E T  I PTLGF+IKT+ ++  +L
Sbjct:   23 GLDNAGKTTLMKKFLDEPTDTIEPTLGFDIKTVHFKDFQL 62

[Close]

BLAST of EY711354 vs. ExPASy Swiss-Prot
Match: ARL2_BOVIN (ADP-ribosylation factor-like protein 2 OS=Bos taurus GN=ARL2 PE=2 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 5.210e-30
Identity = 38/54 (70.37%), Postives = 47/54 (87.04%), Query Frame = 2

Query:  413 YTLNIWDVGGQRTIRSYWRNYFEQTDGLVWVVDSSDLRRLDDCKMELDNLLKEE 574
            + LNIWDVGGQ+++RSYWRNYFE TDGL+WVVDS+D +R+ DC+ EL NLL EE
Sbjct:   60 FKLNIWDVGGQKSLRSYWRNYFESTDGLIWVVDSADRQRMQDCQRELQNLLVEE 113

HSP 2 Score: 60.8474 bits (146), Expect = 5.210e-30
Identity = 31/72 (43.06%), Postives = 46/72 (63.89%), Query Frame = 1

Query:  580 LTNMFQCQKLSGASLLILXNKQDINGALTPTEIAKVLDXGAMDKTRHWKIVGCSAYTSEGLLXGFDXXVXDM 795
            L N+   ++L+GA+LLI  NKQD+ GAL+   I + L+  ++ ++ HW I GCSA T E LL G D  + D+
Sbjct:  106 LQNLLVEERLAGATLLIFANKQDLPGALSSNAIREALELDSI-RSHHWCIQGCSAVTGENLLPGIDWLLDDI 176

[Close]

BLAST of EY711354 vs. ExPASy Swiss-Prot
Match: ARL2_HUMAN (ADP-ribosylation factor-like protein 2 OS=Homo sapiens GN=ARL2 PE=1 SV=4)

HSP 1 Score: 90.1225 bits (222), Expect = 2.539e-29
Identity = 37/54 (68.52%), Postives = 47/54 (87.04%), Query Frame = 2

Query:  413 YTLNIWDVGGQRTIRSYWRNYFEQTDGLVWVVDSSDLRRLDDCKMELDNLLKEE 574
            + LNIWDVGGQ+++RSYWRNYFE TDGL+WVVDS+D +R+ DC+ EL +LL EE
Sbjct:   60 FKLNIWDVGGQKSLRSYWRNYFESTDGLIWVVDSADRQRMQDCQRELQSLLVEE 113

HSP 2 Score: 60.4622 bits (145), Expect = 2.539e-29
Identity = 30/65 (46.15%), Postives = 44/65 (67.69%), Query Frame = 1

Query:  601 QKLSGASLLILXNKQDINGALTPTEIAKVLDXGAMDKTRHWKIVGCSAYTSEGLLXGFDXXVXDM 795
            ++L+GA+LLI  NKQD+ GAL+   I +VL+  ++ ++ HW I GCSA T E LL G D  + D+
Sbjct:  113 ERLAGATLLIFANKQDLPGALSSNAIREVLELDSI-RSHHWCIQGCSAVTGENLLPGIDWLLDDI 176

[Close]

BLAST of EY711354 vs. ExPASy Swiss-Prot
Match: ARL2_RAT (ADP-ribosylation factor-like protein 2 OS=Rattus norvegicus GN=Arl2 PE=2 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 4.305e-29
Identity = 37/54 (68.52%), Postives = 47/54 (87.04%), Query Frame = 2

Query:  413 YTLNIWDVGGQRTIRSYWRNYFEQTDGLVWVVDSSDLRRLDDCKMELDNLLKEE 574
            + LNIWDVGGQ+++RSYWRNYFE TDGL+WVVDS+D +R+ DC+ EL +LL EE
Sbjct:   60 FKLNIWDVGGQKSLRSYWRNYFESTDGLIWVVDSADRQRMQDCQRELQSLLVEE 113

HSP 2 Score: 59.6918 bits (143), Expect = 4.305e-29
Identity = 29/65 (44.62%), Postives = 44/65 (67.69%), Query Frame = 1

Query:  601 QKLSGASLLILXNKQDINGALTPTEIAKVLDXGAMDKTRHWKIVGCSAYTSEGLLXGFDXXVXDM 795
            ++L+GA+LLI  NKQD+ GAL+   I + L+  ++ ++ HW+I GCSA T E LL G D  + D+
Sbjct:  113 ERLAGATLLIFANKQDLPGALSCNAIQEALELDSI-RSHHWRIQGCSAVTGEDLLPGIDWLLDDI 176

[Close]

BLAST of EY711354 vs. ExPASy Swiss-Prot
Match: ARL2_MOUSE (ADP-ribosylation factor-like protein 2 OS=Mus musculus GN=Arl2 PE=1 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 4.305e-29
Identity = 37/54 (68.52%), Postives = 47/54 (87.04%), Query Frame = 2

Query:  413 YTLNIWDVGGQRTIRSYWRNYFEQTDGLVWVVDSSDLRRLDDCKMELDNLLKEE 574
            + LNIWDVGGQ+++RSYWRNYFE TDGL+WVVDS+D +R+ DC+ EL +LL EE
Sbjct:   60 FKLNIWDVGGQKSLRSYWRNYFESTDGLIWVVDSADRQRMQDCQRELQSLLVEE 113

HSP 2 Score: 59.6918 bits (143), Expect = 4.305e-29
Identity = 29/65 (44.62%), Postives = 44/65 (67.69%), Query Frame = 1

Query:  601 QKLSGASLLILXNKQDINGALTPTEIAKVLDXGAMDKTRHWKIVGCSAYTSEGLLXGFDXXVXDM 795
            ++L+GA+LLI  NKQD+ GAL+   I + L+  ++ ++ HW+I GCSA T E LL G D  + D+
Sbjct:  113 ERLAGATLLIFANKQDLPGALSCNAIQEALELDSI-RSHHWRIQGCSAVTGEDLLPGIDWLLDDI 176

[Close]

BLAST of EY711354 vs. ExPASy Swiss-Prot
Match: ARF11_CAEEL (ADP-ribosylation factor 1-like 1 OS=Caenorhabditis elegans GN=arf-1.1 PE=3 SV=3)

HSP 1 Score: 66.6254 bits (161), Expect = 5.668e-26
Identity = 30/56 (53.57%), Postives = 40/56 (71.43%), Query Frame = 2

Query:  404 FDRYTLNIWDVGGQRTIRSYWRNYFEQTDGLVWVVDSSDLRRLDDCKMELDNLLKE 571
            F + TL +WDVGGQ+ IR+ W+ YF  T  LV+VVDSSD+ R+ + K EL +LL E
Sbjct:   58 FQKITLTVWDVGGQKKIRALWKYYFPNTTTLVFVVDSSDIERIPEAKEELFSLLAE 113

HSP 2 Score: 47.3654 bits (111), Expect = 5.668e-26
Identity = 22/56 (39.29%), Postives = 37/56 (66.07%), Query Frame = 1

Query:  604 KLSGASLLILXNKQDINGALTPTEIAKVLDXGAMDKTRHWKIVGCSAYTSEGLLXG 771
            +L+ + LL+  NKQD+  A +P E+ ++LD G++ K R W I G +A++ +GL  G
Sbjct:  115 ELADSHLLVFANKQDMPNARSPAELTQLLDLGSL-KNREWFICGTNAHSGQGLYEG 169

HSP 3 Score: 45.4394 bits (106), Expect = 5.668e-26
Identity = 19/37 (51.35%), Postives = 28/37 (75.68%), Query Frame = 1

Query:  175 GLDNSGKTTIVLKINGEDTSVISPTLGFNIKTITYQK 285
            GLD +GKTTI+ K+   +T    PT+GFN++T+T+QK
Sbjct:   24 GLDGAGKTTILYKLKLNETVNTIPTIGFNVETVTFQK 60

[Close]

BLAST of EY711354 vs. ExPASy Swiss-Prot
Match: ARFK_DICDI (ADP-ribosylation factor K OS=Dictyostelium discoideum GN=arrK PE=3 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 5.898e-25
Identity = 24/57 (42.11%), Postives = 42/57 (73.68%), Query Frame = 2

Query:  404 FDRYTLNIWDVGGQRTIRSYWRNYFEQTDGLVWVVDSSDLRRLDDCKMELDNLLKEE 574
            +   ++ +WDVGGQ  IR+ W++Y+  T+ +++VVDS+D  R+D+ K E+DNLL ++
Sbjct:   66 YKNLSMTVWDVGGQHKIRALWKHYYHGTNAIIFVVDSTDRERMDEVKEEIDNLLIQD 122

HSP 2 Score: 51.2174 bits (121), Expect = 5.898e-25
Identity = 25/66 (37.88%), Postives = 38/66 (57.58%), Query Frame = 1

Query:  580 LTNMFQCQKLSGASLLILXNKQDINGALTPTEIAKVLDXGAMDKTRHWKIVGCSAYTSEGLLXGFD 777
            + N+    +L G  +L+L NKQD+N A+   EI   L+  ++ K R W +  CSA  S+G+  GFD
Sbjct:  115 IDNLLIQDELKGIQILVLANKQDMNNAMNTAEIVNSLNLNSI-KDRKWYVQPCSAIRSDGIYEGFD 179

HSP 3 Score: 40.4318 bits (93), Expect = 5.898e-25
Identity = 16/36 (44.44%), Postives = 26/36 (72.22%), Query Frame = 1

Query:  175 GLDNSGKTTIVLKINGEDTSVISPTLGFNIKTITYQ 282
            GLD +GK+T++ K+   D  +  PT+GFN++TI Y+
Sbjct:   32 GLDGAGKSTLLFKLKLGDVVLTIPTIGFNVETIVYK 67

The following BLAST results are available for this feature:

BLAST of EY711354 vs. ExPASy Swiss-Prot

BLAST of EY711354 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 104


Match Name	E-value	Identity	Description
ARL2_DROME	2.367e-38	68.97	ADP-ribosylation factor-like protein 2 OS=Drosophi...	[more]
ARL2_CAEBR	1.339e-34	63.16	ADP-ribosylation factor-like protein 2 OS=Caenorha...	[more]
ARF5_ARATH	1.341e-34	46.38	Probable ADP-ribosylation factor At2g18390 OS=Arab...	[more]
ARL2_CAEEL	1.490e-32	63.16	ADP-ribosylation factor-like protein 2 OS=Caenorha...	[more]
ARL2_BOVIN	5.210e-30	70.37	ADP-ribosylation factor-like protein 2 OS=Bos taur...	[more]
ARL2_HUMAN	2.539e-29	68.52	ADP-ribosylation factor-like protein 2 OS=Homo sap...	[more]
ARL2_RAT	4.305e-29	68.52	ADP-ribosylation factor-like protein 2 OS=Rattus n...	[more]
ARL2_MOUSE	4.305e-29	68.52	ADP-ribosylation factor-like protein 2 OS=Mus musc...	[more]
ARF11_CAEEL	5.668e-26	53.57	ADP-ribosylation factor 1-like 1 OS=Caenorhabditis...	[more]
ARFK_DICDI	5.898e-25	42.11	ADP-ribosylation factor K OS=Dictyostelium discoid...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C3-702-006-G04-CT.F Sweet orange fruit, development stadium (3 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY711354 ID=EY711354; Name=EY711354; organism=Citrus sinensis; type=EST; length=918bp
TTGCCGACCCACGCGTCCGCCCACGCGTCCTGAAAAAAGAGAAGGAAATG
CGCATTCTTATGGTGTATGTAATTAACTATTTTTCTTTTGTTTGTTTACA
ATCGTGTTTATAATTGTGCGGAACTGTTAATTATTTATTATTTGTTAATT
ATTTGTTTTGTTTTGTTTTGAAGGGGTCTTGATAATTCTGGGAAGACCAC
TATTGTTTTGAAGATAAACGGAGAAGATACTAGCGTTATCAGTCCTACAC
TTGGATTCAATATCAAAACCATTACTTACCAAAAGTAAGAACTTTTTATT
CCATGATTTAGGATCATTTTATGTCAGCCAAAAATGAGAATTTGAAGTCT
TTAAACGCTGTTTAGTTTTTAGCGCGAACATTTTAGTTCCGATATTTTTT
GAATTTGACAGGTACACTTTAAACATATGGGATGTAGGAGGCCAAAGGAC
TATAAGATCATATTGGAGGAACTACTTTGAGCAAACTGATGGTTTGGTGT
GGGTGGTTGATAGTTCCGATCTTAGAAGACTGGATGATTGCAAAATGGAA
CTAGACAATCTTTTAAAGGAAGAGGGAGTCTGACCAACATGTTTCAATGT
CAGAAGCTATCAGGGGCATCCTTACTGATTCTAGNCAATAAGCAGGACAT
CAATGGTGCTCTTACCCCNACAGAGATTGCTAAAGTTCTTGACTTNGGAG
CCATGGACAAAACTCGACACTGGAAAATTGTGGGTTGCAGTGCCTACACA
AGTGAGGGACTGCTAGANGGATTTGATTGNNTAGTTCANGACATGCCTCC
CGGATCTACCTCTTGACTACCCGTTGTTGTACAAGAAAATTTTCTAATTT
TTCCTTGAATGTGAAAAAAAAAGTGTGTTTATTGGACAAACATTGTGAAT
GTATCCCATGTGATTGAT