EY710949 | Citrus Genome Database

Overview


Name	EY710949
Unique Name	EY710949
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	941

Libraries


Library Name	Type
Sweet orange fruit, development stadium (3 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
EY710949_ssr377	microsatellite	EY710949_ssr377:377..396.	BLAST: Citrus ESTs to Prunus persica proteins V1

Homology

[Close]

BLAST of EY710949 vs. ExPASy Swiss-Prot
Match: CSLB4_ARATH (Cellulose synthase-like protein B4 OS=Arabidopsis thaliana GN=CSLB4 PE=2 SV=1)

HSP 1 Score: 146.362 bits (368), Expect = 1.319e-51
Identity = 67/87 (77.01%), Postives = 75/87 (86.21%), Query Frame = 2

Query:  413 RIQELPPVDMFVTTADAELEPPIITVNTVLSLLAVDYPVHKLACYVSDDACSPLNFYSLVEASKFAKLWGPFCKKYNVXIRAPFRYF 673
            R+ ELPPVDMFVTTAD   EPP+I VNTVLSLLAV+YP +KLACYVSDD CSPL ++SL EASKFAK+W PFCKKYNV +RAPF YF
Sbjct:   88 RVHELPPVDMFVTTADPVREPPLIVVNTVLSLLAVNYPANKLACYVSDDGCSPLTYFSLKEASKFAKIWVPFCKKYNVRVRAPFMYF 174

HSP 2 Score: 78.9518 bits (193), Expect = 1.319e-51
Identity = 36/88 (40.91%), Postives = 47/88 (53.41%), Query Frame = 3

Query:   39 MANTKSSCLPLYEKVFHKNTYHRAXXXXXXXXXXXXXXXXXXXXKNHGFAWFLAFLCESCFTFIWILIVNCKWTPLTYKTYPQRLQER 302
            MA + S   PL E++ HK+ + RA                          W +AFLCE+CFTF+W+LI N KW+P  YKTYP+RL ER
Sbjct:    1 MAESSSPLPPLCERISHKSYFLRAVDLTILGLLLSLLLYRILHVNQKDTVWIVAFLCETCFTFVWLLITNIKWSPADYKTYPERLDER 88

[Close]

BLAST of EY710949 vs. ExPASy Swiss-Prot
Match: CSLB3_ARATH (Cellulose synthase-like protein B3 OS=Arabidopsis thaliana GN=CSLB3 PE=2 SV=1)

HSP 1 Score: 141.354 bits (355), Expect = 9.943e-49
Identity = 63/87 (72.41%), Postives = 74/87 (85.06%), Query Frame = 2

Query:  413 RIQELPPVDMFVTTADAELEPPIITVNTVLSLLAVDYPVHKLACYVSDDACSPLNFYSLVEASKFAKLWGPFCKKYNVXIRAPFRYF 673
            R+ +LP VDMFVTTAD   EPPI+  NT+LSLLAV+YP +KLACYVSDD CSPL ++SL EASKFAK+W PFCKKYN+ +RAPFRYF
Sbjct:   88 RVHDLPSVDMFVTTADPVREPPILVANTLLSLLAVNYPANKLACYVSDDGCSPLTYFSLKEASKFAKIWVPFCKKYNIKVRAPFRYF 174

HSP 2 Score: 74.3294 bits (181), Expect = 9.943e-49
Identity = 35/88 (39.77%), Postives = 49/88 (55.68%), Query Frame = 3

Query:   39 MANTKSSCLPLYEKVFHKNTYHRAXXXXXXXXXXXXXXXXXXXXKNHGFAWFLAFLCESCFTFIWILIVNCKWTPLTYKTYPQRLQER 302
            MA++ SS  PL EK+ +KN + R                       +   W +AFLCES F+FIW+LI + KW+P +YK+YP+RL ER
Sbjct:    1 MADSSSSLPPLCEKISYKNYFLRVVDLTILGFLFSLLLYRILLMNQNNSVWVVAFLCESFFSFIWLLITSIKWSPASYKSYPERLDER 88

[Close]

BLAST of EY710949 vs. ExPASy Swiss-Prot
Match: CSLB2_ARATH (Cellulose synthase-like protein B2 OS=Arabidopsis thaliana GN=CSLB2 PE=2 SV=1)

HSP 1 Score: 140.198 bits (352), Expect = 1.350e-47
Identity = 64/87 (73.56%), Postives = 74/87 (85.06%), Query Frame = 2

Query:  413 RIQELPPVDMFVTTADAELEPPIITVNTVLSLLAVDYPVHKLACYVSDDACSPLNFYSLVEASKFAKLWGPFCKKYNVXIRAPFRYF 673
            R+ +LP VDMFV TAD   EPPI+ VNTVLSLLAV+YP +KLACYVSDD CSPL ++SL EASKFAK+W PFCKKYN+ +RAPFRYF
Sbjct:   88 RVYDLPSVDMFVPTADPVREPPIMVVNTVLSLLAVNYPANKLACYVSDDGCSPLTYFSLKEASKFAKIWVPFCKKYNLKVRAPFRYF 174

HSP 2 Score: 69.3218 bits (168), Expect = 1.350e-47
Identity = 33/88 (37.50%), Postives = 44/88 (50.00%), Query Frame = 3

Query:   39 MANTKSSCLPLYEKVFHKNTYHRAXXXXXXXXXXXXXXXXXXXXKNHGFAWFLAFLCESCFTFIWILIVNCKWTPLTYKTYPQRLQER 302
            MA++  S  PLYE + +K+   RA                      +G  W +AFLCESCF+F+W+L    KW+P   K YP RL ER
Sbjct:    1 MADSSCSLPPLYENISYKSYILRAVDLTILGLLFSLLLHRILYMSQNGIIWLVAFLCESCFSFVWLLSTCTKWSPAETKPYPDRLDER 88

HSP 3 Score: 23.0978 bits (48), Expect = 1.350e-47
Identity = 13/35 (37.14%), Postives = 20/35 (57.14%), Query Frame = 3

Query:  705 GDSLQTGKNMKXNDXFLHTERMNHPNIVKVYXENK 809
            GDS   G + +  + F +T+  +H  I+KV  ENK
Sbjct:  209 GDSHLLGTDNEL-EAFSNTKPNDHSTIIKVVWENK 242

[Close]

BLAST of EY710949 vs. ExPASy Swiss-Prot
Match: CSLB6_ARATH (Cellulose synthase-like protein B6 OS=Arabidopsis thaliana GN=CSLB6 PE=2 SV=2)

HSP 1 Score: 142.51 bits (358), Expect = 4.058e-47
Identity = 65/87 (74.71%), Postives = 73/87 (83.91%), Query Frame = 2

Query:  413 RIQELPPVDMFVTTADAELEPPIITVNTVLSLLAVDYPVHKLACYVSDDACSPLNFYSLVEASKFAKLWGPFCKKYNVXIRAPFRYF 673
            R+ +LP VDMFV TAD   EPPII VNTVLSLLAV+YP +KLACYVSDD CSPL ++SL EASKF K+W PFCKKYNV +RAPFRYF
Sbjct:   88 RVHDLPSVDMFVPTADPVREPPIIVVNTVLSLLAVNYPANKLACYVSDDGCSPLTYFSLKEASKFVKIWAPFCKKYNVRVRAPFRYF 174

HSP 2 Score: 67.781 bits (164), Expect = 4.058e-47
Identity = 33/88 (37.50%), Postives = 45/88 (51.14%), Query Frame = 3

Query:   39 MANTKSSCLPLYEKVFHKNTYHRAXXXXXXXXXXXXXXXXXXXXKNHGFAWFLAFLCESCFTFIWILIVNCKWTPLTYKTYPQRLQER 302
            MA++ SS LPL E++ HK+   R                       +   W +AFLCESCF+F+W++I   KW+P   K YP RL ER
Sbjct:    1 MADSSSSLLPLCERISHKSYILRIVDLTILVLLFSLLWYRILHMCENNTIWLVAFLCESCFSFMWLIITCIKWSPAEDKPYPNRLDER 88

[Close]

BLAST of EY710949 vs. ExPASy Swiss-Prot
Match: CSLB1_ARATH (Cellulose synthase-like protein B1 OS=Arabidopsis thaliana GN=CSLB1 PE=2 SV=2)

HSP 1 Score: 139.428 bits (350), Expect = 8.397e-43
Identity = 66/110 (60.00%), Postives = 78/110 (70.91%), Query Frame = 2

Query:  413 RIQELPPVDMFVTTADAELEPPIITVNTVLSLLAVDYPVHKLACYVSDDACSPLNFYSLVEASKFAKLWGPFCKKYNVXIRAPFRYFXXXXXXXXXXXXLPADWKKHEXE 742
            R+ +LP VDMFV TAD   EPPI+ V+TVLSLLAV+YP +KLACYVSDD CSPL ++SL EASKFAK+W PFCKKYN  +RAP RYF               DW+K + E
Sbjct:   88 RVHDLPSVDMFVPTADPVREPPIMVVDTVLSLLAVNYPANKLACYVSDDGCSPLTYFSLKEASKFAKIWVPFCKKYNTRVRAPSRYFLKPISVATEDYEFNRDWEKTKRE 197

HSP 2 Score: 55.0694 bits (131), Expect = 8.397e-43
Identity = 28/88 (31.82%), Postives = 40/88 (45.45%), Query Frame = 3

Query:   39 MANTKSSCLPLYEKVFHKNTYHRAXXXXXXXXXXXXXXXXXXXXKNHGFAWFLAFLCESCFTFIWILIVNCKWTPLTYKTYPQRLQER 302
            MA++  S  PL E++ + N + RA                      +   W +AF CESCF  + +LI   KW+P   K +P RL ER
Sbjct:    1 MADSSFSLPPLCERISYTNYFLRAVYLTVLGLFFSLLLHRIRHTSEYDNVWLVAFFCESCFFLVCLLITCLKWSPADTKPFPDRLDER 88

HSP 3 Score: 21.9422 bits (45), Expect = 8.397e-43
Identity = 9/22 (40.91%), Postives = 14/22 (63.64%), Query Frame = 3

Query:  744 DXFLHTERMNHPNIVKVYXENK 809
            + F +T+  +H  +VKV  ENK
Sbjct:  222 EAFSNTKPNDHSTLVKVVWENK 243

[Close]

BLAST of EY710949 vs. ExPASy Swiss-Prot
Match: CSLH1_ORYSJ (Cellulose synthase-like protein H1 OS=Oryza sativa subsp. japonica GN=CSLH1 PE=2 SV=2)

HSP 1 Score: 132.494 bits (332), Expect = 1.601e-40
Identity = 64/106 (60.38%), Postives = 75/106 (70.75%), Query Frame = 2

Query:  413 RIQELPPVDMFVTTADAELEPPIITVNTVLSLLAVDYPV--HKLACYVSDDACSPLNFYSLVEASKFAKLWGPFCKKYNVXIRAPFRYFXXXXXXXXXXXXLPADW 724
            RI ELP VDMFVTTAD  LEPP++TVNTVLSLLA+DYP    KLACYVSDD CSPL  Y+L EA++FA+ W PFC+++ V +RAPFRYF               DW
Sbjct:   87 RIDELPAVDMFVTTADPVLEPPLVTVNTVLSLLALDYPAAGEKLACYVSDDGCSPLTCYALREAARFARTWVPFCRRHGVAVRAPFRYFSSTPEFGPADGKFLEDW 192

HSP 2 Score: 48.521 bits (114), Expect = 1.601e-40
Identity = 18/35 (51.43%), Postives = 26/35 (74.29%), Query Frame = 3

Query:  198 AFLCESCFTFIWILIVNCKWTPLTYKTYPQRLQER 302
            A  CE+ FTF+W+L VN KW+P+ + T+P+ L ER
Sbjct:   53 ALACEAWFTFMWLLNVNAKWSPVRFDTFPENLAER 87

HSP 3 Score: 27.7202 bits (60), Expect = 1.601e-40
Identity = 21/59 (35.59%), Postives = 26/59 (44.07%), Query Frame = 3

Query:  750 FLHTERMNHPNIVKVYXENKEVSR*DN--TFSYYSERKAK--HPLTTSRAMNX*LRLXA 914
            FL  ER NHP I+KV  +N      D      Y S  K+   H    + AMN   R+ A
Sbjct:  225 FLDVERGNHPTIIKVLWDNNRSRTGDGFPRLIYVSREKSPNLHHHYKAGAMNALTRVSA 283

[Close]

BLAST of EY710949 vs. ExPASy Swiss-Prot
Match: CSLB5_ARATH (Cellulose synthase-like protein B5 OS=Arabidopsis thaliana GN=CSLB5 PE=2 SV=2)

HSP 1 Score: 142.124 bits (357), Expect = 4.333e-33
Identity = 70/122 (57.38%), Postives = 85/122 (69.67%), Query Frame = 2

Query:  320 LALIISASFAIVFVILQVXXXXXXXXCVRLN----RIQELPPVDMFVTTADAELEPPIITVNTVLSLLAVDYPVHKLACYVSDDACSPLNFYSLVEASKFAKLWGPFCKKYNVXIRAPFRYF 673
            LA    + F++V++I              +N    R+ +LP +DMFV TAD   E PIITVNTVLSLLAV+YP +KLACYVSDD CSPL ++SL EASKF K+W PFCKKYNV +RAPFRYF
Sbjct:   53 LAFFCESCFSLVWLIFTCLKWSPAEDIPYINTLNERVHDLPSLDMFVPTADTVRESPIITVNTVLSLLAVNYPANKLACYVSDDGCSPLTYFSLKEASKFVKIWAPFCKKYNVRVRAPFRYF 174

[Close]

BLAST of EY710949 vs. ExPASy Swiss-Prot
Match: CESA4_ARATH (Cellulose synthase A catalytic subunit 4 [UDP-forming] OS=Arabidopsis thaliana GN=CESA4 PE=1 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 2.110e-28
Identity = 54/84 (64.29%), Postives = 65/84 (77.38%), Query Frame = 2

Query:  422 ELPPVDMFVTTADAELEPPIITVNTVLSLLAVDYPVHKLACYVSDDACSPLNFYSLVEASKFAKLWGPFCKKYNVXIRAPFRYF 673
            +L PVD+FV+T D   EPPIIT NT+LS+LAVDYPV+K++CYVSDD  S L F +L E S+FA+ W PFCKKYNV  RAP  YF
Sbjct:  290 KLAPVDVFVSTVDPLKEPPIITANTILSILAVDYPVNKVSCYVSDDGASMLLFDTLSETSEFARRWVPFCKKYNVEPRAPEFYF 373

HSP 2 Score: 35.4242 bits (80), Expect = 2.110e-28
Identity = 15/38 (39.47%), Postives = 20/38 (52.63%), Query Frame = 3

Query:  189 WFLAFLCESCFTFIWILIVNCKWTPLTYKTYPQRLQER 302
            W ++ +CE  F   WIL    KW P+  +TY  RL  R
Sbjct:  244 WLISVICEIWFALSWILDQFPKWFPINRETYLDRLSMR 281

[Close]

BLAST of EY710949 vs. ExPASy Swiss-Prot
Match: CESA6_ORYSJ (Probable cellulose synthase A catalytic subunit 6 [UDP-forming] OS=Oryza sativa subsp. japonica GN=CESA6 PE=2 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 3.574e-28
Identity = 50/84 (59.52%), Postives = 63/84 (75.00%), Query Frame = 2

Query:  422 ELPPVDMFVTTADAELEPPIITVNTVLSLLAVDYPVHKLACYVSDDACSPLNFYSLVEASKFAKLWGPFCKKYNVXIRAPFRYF 673
            +L PVD FV+T D   EPP++T NTVLS+L+VDYPV K++CYVSDD  + L F +L E S+FAK W PFCKK+N+  RAP  YF
Sbjct:  354 QLAPVDFFVSTVDPSKEPPLVTANTVLSILSVDYPVEKVSCYVSDDGAAMLTFEALSETSEFAKKWVPFCKKFNIEPRAPEWYF 437

HSP 2 Score: 36.1946 bits (82), Expect = 3.574e-28
Identity = 18/44 (40.91%), Postives = 23/44 (52.27%), Query Frame = 3

Query:  174 NHGFA-WFLAFLCESCFTFIWILIVNCKWTPLTYKTYPQRLQER 302
            N  FA W ++ +CE  F   WIL    KW P+  +TY  RL  R
Sbjct:  302 NDAFALWLISVICEIWFAMSWILDQFPKWLPIERETYLDRLSLR 345

[Close]

BLAST of EY710949 vs. ExPASy Swiss-Prot
Match: CESA2_ORYSJ (Probable cellulose synthase A catalytic subunit 2 [UDP-forming] OS=Oryza sativa subsp. japonica GN=CESA2 PE=2 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 4.654e-28
Identity = 50/84 (59.52%), Postives = 64/84 (76.19%), Query Frame = 2

Query:  422 ELPPVDMFVTTADAELEPPIITVNTVLSLLAVDYPVHKLACYVSDDACSPLNFYSLVEASKFAKLWGPFCKKYNVXIRAPFRYF 673
            +L PVD+FV+T D   EPP++T NTVLS+LAVDYPV K++CYVSDD  + L F +L E S+FA+ W PFCKKY++  RAP  YF
Sbjct:  344 QLAPVDIFVSTVDPMKEPPLVTANTVLSILAVDYPVDKVSCYVSDDGAAMLTFDALAETSEFARKWVPFCKKYSIEPRAPEWYF 427

HSP 2 Score: 36.5798 bits (83), Expect = 4.654e-28
Identity = 16/38 (42.11%), Postives = 21/38 (55.26%), Query Frame = 3

Query:  189 WFLAFLCESCFTFIWILIVNCKWTPLTYKTYPQRLQER 302
            W L+ +CE  F   WIL    KW+P+  +TY  RL  R
Sbjct:  298 WLLSVICEIWFALSWILDQFPKWSPINRETYLDRLALR 335

The following BLAST results are available for this feature:

BLAST of EY710949 vs. ExPASy Swiss-Prot

BLAST of EY710949 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 65


Match Name	E-value	Identity	Description
CSLB4_ARATH	1.319e-51	77.01	Cellulose synthase-like protein B4 OS=Arabidopsis ...	[more]
CSLB3_ARATH	9.943e-49	72.41	Cellulose synthase-like protein B3 OS=Arabidopsis ...	[more]
CSLB2_ARATH	1.350e-47	73.56	Cellulose synthase-like protein B2 OS=Arabidopsis ...	[more]
CSLB6_ARATH	4.058e-47	74.71	Cellulose synthase-like protein B6 OS=Arabidopsis ...	[more]
CSLB1_ARATH	8.397e-43	60.00	Cellulose synthase-like protein B1 OS=Arabidopsis ...	[more]
CSLH1_ORYSJ	1.601e-40	60.38	Cellulose synthase-like protein H1 OS=Oryza sativa...	[more]
CSLB5_ARATH	4.333e-33	57.38	Cellulose synthase-like protein B5 OS=Arabidopsis ...	[more]
CESA4_ARATH	2.110e-28	64.29	Cellulose synthase A catalytic subunit 4 [UDP-form...	[more]
CESA6_ORYSJ	3.574e-28	59.52	Probable cellulose synthase A catalytic subunit 6 ...	[more]
CESA2_ORYSJ	4.654e-28	59.52	Probable cellulose synthase A catalytic subunit 2 ...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C3-702-002-A03-CT.F Sweet orange fruit, development stadium (3 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY710949 ID=EY710949; Name=EY710949; organism=Citrus sinensis; type=EST; length=941bp
CCACGCGTCCGCGGACGCGTGGGTTGTTTTTCAAGCTCATGGCCAATACA
AAGTCGTCATGTCTCCCTCTATACGAAAAAGTTTTTCATAAAAACACATA
TCATAGGGCTTTAGATGTTATCATCTTGTTCCTTCTTCTATGTCTGCTCT
TCTATCGGCTCTCAACGTTGAAGAATCATGGATTTGCTTGGTTTCTTGCT
TTTCTATGTGAGTCTTGTTTTACTTTCATCTGGATTCTCATCGTCAACTG
CAAGTGGACTCCTTTGACCTACAAAACATATCCCCAACGTCTCCAAGAAA
GGTAACAAATCAAATAACGTTAGCATTAATAATTTCCGCATCATTTGCAA
TTGTCTTTGTCATTTTGCAAGTTACTACACACACACACACACACACATGT
GTACGACTCAACAGGATACAGGAGCTGCCCCCAGTGGACATGTTTGTGAC
AACTGCTGATGCTGAACTCGAGCCGCCAATCATCACTGTAAACACAGTGC
TGTCTTTGTTGGCAGTTGATTATCCAGTTCACAAGCTCGCTTGCTATGTT
TCTGATGATGCCTGCTCACCTCTCAATTTCTACTCTCTTGTTGAAGCTTC
TAAATTCGCTAAGCTTTGGGGTCCATTCTGTAAAAAGTATAATGTTCNAA
TTAGAGCTCCCTTTAGATACTTCCCAGATAGTGAGCCGTCCGATTCCAGN
TCAGGGGGACTCCCTGCAGACTGGAAAAAACATGAAGANGAATGACTGNT
TTCTCCATACAGAACGCATGAATCATCCCAACATAGTGAAGGTATATNCN
GAGAACAAGGAAGTCTCTCGATGAGATAACACATTTAGTTACTATTCAGA
GAGAAAAGCCAAGCATCCCCTCACTACAAGCAGGGCCATGAATGNATGAC
TGCGACTGNATGCCCACATCCAGAGATGGTCTTCAGCTTTG