EY746125

Overview
NameEY746125
Unique NameEY746125
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length807
Libraries
Library NameType
Sweet orange flower, greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY746125 vs. ExPASy Swiss-Prot
Match: PLDA1_RICCO (Phospholipase D alpha 1 OS=Ricinus communis GN=PLD1 PE=1 SV=1)

HSP 1 Score: 324.709 bits (831), Expect = 1.678e-94
Identity = 150/171 (87.72%), Postives = 164/171 (95.91%), Query Frame = 3 
Query:    3 TFFCLGNREVKRSGEYEPAERPEDDSDYLRAQEARRFMIYVHAKMMIVDDEYIIIGSANINQRSMEGARDTEIAMGGYQPYHLSTRVPARGQIHGFRMSLWYEHLGMLDDTFLQPESSECVKKVNQIAERYWDLYSSESLEHDLPGHLLRYPIGVSGEGDVTELPGTEFFP 515
            TFFCLGNREVK+SGEYEPAE+PE D+DY+RAQEARRFMIYVH KMMIVDDEYIIIGSANINQRSM+GARD+EIAMG YQP+HLSTR PARGQIHGFRMSLWYEHLGMLD++FL PES ECV+KVNQ+AE+YWDLYSSE+LEHDLPGHLLRYPIGV+ EGDVTELPGTEFFP
Sbjct:  618 TFFCLGNREVKKSGEYEPAEKPEPDTDYIRAQEARRFMIYVHTKMMIVDDEYIIIGSANINQRSMDGARDSEIAMGAYQPHHLSTRQPARGQIHGFRMSLWYEHLGMLDESFLNPESEECVRKVNQMAEKYWDLYSSETLEHDLPGHLLRYPIGVASEGDVTELPGTEFFP 788

HSP 2 Score: 43.5134 bits (101), Expect = 1.678e-94
Identity = 18/21 (85.71%), Postives = 20/21 (95.24%), Query Frame = 1 
Query:  514 PDTKARVLGTKSDYMPPVLTT 576
            PDTKARVLG KSDY+PP+LTT
Sbjct:  788 PDTKARVLGAKSDYLPPILTT 808
[Close]
BLAST of EY746125 vs. ExPASy Swiss-Prot
Match: PLDA2_ARATH (Phospholipase D alpha 2 OS=Arabidopsis thaliana GN=PLDALPHA2 PE=2 SV=1)

HSP 1 Score: 319.316 bits (817), Expect = 4.076e-93
Identity = 144/171 (84.21%), Postives = 162/171 (94.74%), Query Frame = 3 
Query:    3 TFFCLGNREVKRSGEYEPAERPEDDSDYLRAQEARRFMIYVHAKMMIVDDEYIIIGSANINQRSMEGARDTEIAMGGYQPYHLSTRVPARGQIHGFRMSLWYEHLGMLDDTFLQPESSECVKKVNQIAERYWDLYSSESLEHDLPGHLLRYPIGVSGEGDVTELPGTEFFP 515
            TFFCLGNREVK+ GEYEP+E+PE D+DY+RAQEARRFMIYVH KMMIVDDEYIIIGSANINQRSM+GARD+EIAMGGYQPYHLSTR PARGQIHGFRMSLWYEHLGMLD+TFL P S EC++KVN++A++YWDLYSSESLEHDLPGHLLRYPIG++ EG++TELPG EFFP
Sbjct:  620 TFFCLGNREVKKDGEYEPSEKPEPDTDYIRAQEARRFMIYVHTKMMIVDDEYIIIGSANINQRSMDGARDSEIAMGGYQPYHLSTRQPARGQIHGFRMSLWYEHLGMLDETFLDPSSQECIQKVNRVADKYWDLYSSESLEHDLPGHLLRYPIGIASEGNITELPGCEFFP 790

HSP 2 Score: 44.2838 bits (103), Expect = 4.076e-93
Identity = 18/21 (85.71%), Postives = 20/21 (95.24%), Query Frame = 1 
Query:  514 PDTKARVLGTKSDYMPPVLTT 576
            PDTKAR+LG KSDYMPP+LTT
Sbjct:  790 PDTKARILGVKSDYMPPILTT 810
[Close]
BLAST of EY746125 vs. ExPASy Swiss-Prot
Match: PLDA1_ARATH (Phospholipase D alpha 1 OS=Arabidopsis thaliana GN=PLDALPHA1 PE=1 SV=2)

HSP 1 Score: 313.538 bits (802), Expect = 1.292e-91
Identity = 143/171 (83.63%), Postives = 160/171 (93.57%), Query Frame = 3 
Query:    3 TFFCLGNREVKRSGEYEPAERPEDDSDYLRAQEARRFMIYVHAKMMIVDDEYIIIGSANINQRSMEGARDTEIAMGGYQPYHLSTRVPARGQIHGFRMSLWYEHLGMLDDTFLQPESSECVKKVNQIAERYWDLYSSESLEHDLPGHLLRYPIGVSGEGDVTELPGTEFFP 515
            TFFCLGNREVK+ GEYEPAE+P+ D+DY+RAQEARRFMIYVH KMMIVDDEYIIIGSANINQRSM+GARD+EIAMGGYQP+HLS R PARGQIHGFRMSLWYEHLGMLD+TFL P S EC++KVN+I+++YWD YSSESLEHDLPGHLLRYPIGV+ EGD+TELPG EFFP
Sbjct:  620 TFFCLGNREVKKDGEYEPAEKPDPDTDYMRAQEARRFMIYVHTKMMIVDDEYIIIGSANINQRSMDGARDSEIAMGGYQPHHLSHRQPARGQIHGFRMSLWYEHLGMLDETFLDPSSLECIEKVNRISDKYWDFYSSESLEHDLPGHLLRYPIGVASEGDITELPGFEFFP 790

HSP 2 Score: 45.0542 bits (105), Expect = 1.292e-91
Identity = 18/21 (85.71%), Postives = 21/21 (100.00%), Query Frame = 1 
Query:  514 PDTKARVLGTKSDYMPPVLTT 576
            PDTKAR+LGTKSDY+PP+LTT
Sbjct:  790 PDTKARILGTKSDYLPPILTT 810
[Close]
BLAST of EY746125 vs. ExPASy Swiss-Prot
Match: PLDA1_BRAOC (Phospholipase D alpha 1 OS=Brassica oleracea var. capitata GN=PLD1 PE=2 SV=1)

HSP 1 Score: 311.227 bits (796), Expect = 6.366e-91
Identity = 143/171 (83.63%), Postives = 158/171 (92.40%), Query Frame = 3 
Query:    3 TFFCLGNREVKRSGEYEPAERPEDDSDYLRAQEARRFMIYVHAKMMIVDDEYIIIGSANINQRSMEGARDTEIAMGGYQPYHLSTRVPARGQIHGFRMSLWYEHLGMLDDTFLQPESSECVKKVNQIAERYWDLYSSESLEHDLPGHLLRYPIGVSGEGDVTELPGTEFFP 515
            TFFCLGNREVK+ GEYEPAERP+ DS Y++AQEARRFMIYVH KMMIVDDEYIIIGSANINQRSM+GARD+EIAMGGYQP+HLS R PARGQIHGFRMSLWYEHLGMLD+TFL P S EC++KVN+I+++YWDLYSSESLEHDLPGHLLRYP+ V GEGDVTE PG EFFP
Sbjct:  620 TFFCLGNREVKKEGEYEPAERPDADSSYMKAQEARRFMIYVHTKMMIVDDEYIIIGSANINQRSMDGARDSEIAMGGYQPHHLSHRQPARGQIHGFRMSLWYEHLGMLDETFLDPSSVECIEKVNRISDKYWDLYSSESLEHDLPGHLLRYPVDVDGEGDVTEFPGFEFFP 790

HSP 2 Score: 45.0542 bits (105), Expect = 6.366e-91
Identity = 18/21 (85.71%), Postives = 21/21 (100.00%), Query Frame = 1 
Query:  514 PDTKARVLGTKSDYMPPVLTT 576
            PDTKAR+LGTKSDY+PP+LTT
Sbjct:  790 PDTKARILGTKSDYLPPILTT 810
[Close]
BLAST of EY746125 vs. ExPASy Swiss-Prot
Match: PLDA1_TOBAC (Phospholipase D alpha 1 OS=Nicotiana tabacum GN=PLD1 PE=2 SV=2)

HSP 1 Score: 309.686 bits (792), Expect = 1.413e-90
Identity = 142/171 (83.04%), Postives = 159/171 (92.98%), Query Frame = 3 
Query:    3 TFFCLGNREVKRSGEYEPAERPEDDSDYLRAQEARRFMIYVHAKMMIVDDEYIIIGSANINQRSMEGARDTEIAMGGYQPYHLSTRVPARGQIHGFRMSLWYEHLGMLDDTFLQPESSECVKKVNQIAERYWDLYSSESLEHDLPGHLLRYPIGVSGEGDVTELPGTEFFP 515
            TFFC+GNREVK+SG YEP+E PE DSDY+RAQEARRFMIYVH+KMMIVDDEYII+GSANINQRSM+GARD+EIAMG YQP+HL+TR PARGQIHGFRM+LWYEHLGMLD+TFL PES ECV KVN++A++YWDLYSSESLE DLPGHLLRYPIGV+ EGDVTELPG E FP
Sbjct:  618 TFFCIGNREVKKSGAYEPSETPEPDSDYIRAQEARRFMIYVHSKMMIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPHHLATREPARGQIHGFRMALWYEHLGMLDETFLHPESEECVSKVNRMADKYWDLYSSESLERDLPGHLLRYPIGVASEGDVTELPGAEHFP 788

HSP 2 Score: 45.4394 bits (106), Expect = 1.413e-90
Identity = 19/21 (90.48%), Postives = 21/21 (100.00%), Query Frame = 1 
Query:  514 PDTKARVLGTKSDYMPPVLTT 576
            PDTKARVLGTKSDY+PP+LTT
Sbjct:  788 PDTKARVLGTKSDYLPPILTT 808
[Close]
BLAST of EY746125 vs. ExPASy Swiss-Prot
Match: PLDA1_VIGUN (Phospholipase D alpha 1 OS=Vigna unguiculata GN=PLD1 PE=2 SV=1)

HSP 1 Score: 311.227 bits (796), Expect = 5.338e-90
Identity = 145/171 (84.80%), Postives = 158/171 (92.40%), Query Frame = 3 
Query:    3 TFFCLGNREVKRSGEYEPAERPEDDSDYLRAQEARRFMIYVHAKMMIVDDEYIIIGSANINQRSMEGARDTEIAMGGYQPYHLSTRVPARGQIHGFRMSLWYEHLGMLDDTFLQPESSECVKKVNQIAERYWDLYSSESLEHDLPGHLLRYPIGVSGEGDVTELPGTEFFP 515
            TFFCLGNREVK+SGEYEPAE+PE DSDY RAQEARRFMIYVH KMMIVDDEYIIIGSANINQRSM+GARD+EIAMGGYQPYHL+   PARGQ++GFRMSLWYEHLGML DTF +PES EC+ KVNQIA++YWDLYSSESLE DLPGHLLRYPIGV+ EG+VTELPG EFFP
Sbjct:  619 TFFCLGNREVKKSGEYEPAEQPEPDSDYQRAQEARRFMIYVHTKMMIVDDEYIIIGSANINQRSMDGARDSEIAMGGYQPYHLANTQPARGQVYGFRMSLWYEHLGMLHDTFQRPESEECINKVNQIADKYWDLYSSESLERDLPGHLLRYPIGVASEGEVTELPGFEFFP 789

HSP 2 Score: 41.9726 bits (97), Expect = 5.338e-90
Identity = 16/21 (76.19%), Postives = 20/21 (95.24%), Query Frame = 1 
Query:  514 PDTKARVLGTKSDYMPPVLTT 576
            PDTKAR+LG K+DY+PP+LTT
Sbjct:  789 PDTKARILGAKADYLPPILTT 809
[Close]
BLAST of EY746125 vs. ExPASy Swiss-Prot
Match: PLDA2_BRAOC (Phospholipase D alpha 2 OS=Brassica oleracea var. capitata GN=PLD2 PE=1 SV=2)

HSP 1 Score: 310.842 bits (795), Expect = 4.476e-89
Identity = 141/171 (82.46%), Postives = 159/171 (92.98%), Query Frame = 3 
Query:    3 TFFCLGNREVKRSGEYEPAERPEDDSDYLRAQEARRFMIYVHAKMMIVDDEYIIIGSANINQRSMEGARDTEIAMGGYQPYHLSTRVPARGQIHGFRMSLWYEHLGMLDDTFLQPESSECVKKVNQIAERYWDLYSSESLEHDLPGHLLRYPIGVSGEGDVTELPGTEFFP 515
            TFFCLGNREVK+ GEYEPAERP+ D+DY+RAQEARRFMIYVH+KMMIVDDEYII+GSANINQRSM+GARD+EIAMGGYQP+HLS R PARGQ+HGFRMSLWYEHLGMLD+TFL P S EC++KVN+IA++YWD YSSESLEHDLPGHLLRYPI V  EG++TELPG EFFP
Sbjct:  622 TFFCLGNREVKKEGEYEPAERPDPDTDYMRAQEARRFMIYVHSKMMIVDDEYIIVGSANINQRSMDGARDSEIAMGGYQPHHLSHRQPARGQVHGFRMSLWYEHLGMLDETFLDPSSLECIEKVNRIADKYWDFYSSESLEHDLPGHLLRYPISVDNEGNITELPGFEFFP 792

HSP 2 Score: 39.2762 bits (90), Expect = 4.476e-89
Identity = 15/21 (71.43%), Postives = 19/21 (90.48%), Query Frame = 1 
Query:  514 PDTKARVLGTKSDYMPPVLTT 576
            PD+KAR+LG K DY+PP+LTT
Sbjct:  792 PDSKARILGNKVDYLPPILTT 812
[Close]
BLAST of EY746125 vs. ExPASy Swiss-Prot
Match: PLDA1_PIMBR (Phospholipase D alpha 1 OS=Pimpinella brachycarpa GN=PLD1 PE=2 SV=1)

HSP 1 Score: 304.679 bits (779), Expect = 4.896e-88
Identity = 140/171 (81.87%), Postives = 159/171 (92.98%), Query Frame = 3 
Query:    3 TFFCLGNREVKRSGEYEPAERPEDDSDYLRAQEARRFMIYVHAKMMIVDDEYIIIGSANINQRSMEGARDTEIAMGGYQPYHLSTRVPARGQIHGFRMSLWYEHLGMLDDTFLQPESSECVKKVNQIAERYWDLYSSESLEHDLPGHLLRYPIGVSGEGDVTELPGTEFFP 515
            TFFCLGNREVKR GEYEP+E+P+ D+DY RAQE+RRFMIYVHAKMMIVDDEYIIIGSANINQRSM+GA+D+EIAMG YQP+HL+TR PARGQIHGFRMSLWYEHLGMLDDT   PES +CV+KVN +A++YWDLYSSE+LE+DLPGHLLRYPI V+ EG+VTELPGTEFFP
Sbjct:  618 TFFCLGNREVKRDGEYEPSEKPDPDTDYSRAQESRRFMIYVHAKMMIVDDEYIIIGSANINQRSMDGAKDSEIAMGAYQPHHLATREPARGQIHGFRMSLWYEHLGMLDDTLALPESVDCVQKVNTVADKYWDLYSSETLENDLPGHLLRYPIAVASEGNVTELPGTEFFP 788

HSP 2 Score: 41.9726 bits (97), Expect = 4.896e-88
Identity = 17/21 (80.95%), Postives = 20/21 (95.24%), Query Frame = 1 
Query:  514 PDTKARVLGTKSDYMPPVLTT 576
            PDTKARVLG KSD++PP+LTT
Sbjct:  788 PDTKARVLGAKSDFLPPILTT 808
[Close]
BLAST of EY746125 vs. ExPASy Swiss-Prot
Match: PLDA1_MAIZE (Phospholipase D alpha 1 OS=Zea mays GN=PLD1 PE=2 SV=1)

HSP 1 Score: 300.827 bits (769), Expect = 5.354e-87
Identity = 139/171 (81.29%), Postives = 154/171 (90.06%), Query Frame = 3 
Query:    3 TFFCLGNREVKRSGEYEPAERPEDDSDYLRAQEARRFMIYVHAKMMIVDDEYIIIGSANINQRSMEGARDTEIAMGGYQPYHLSTRVPARGQIHGFRMSLWYEHLGMLDDTFLQPESSECVKKVNQIAERYWDLYSSESLEHDLPGHLLRYPIGVSGEGDVTELPGTEFFP 515
            TFFCLGNREVK+ GEYEP E PE D+DY+RAQEARRFMIYVH KMMIVDDEYIIIGSANINQRSM+GARD+EIAMG YQPYHL+TR PARGQIHGFRMSLWYEHLGML+D F +PES ECV+KVN++AE+YWDLYSS+ LE DLPGHLL YPIGV+ +G VTELPG E FP
Sbjct:  622 TFFCLGNREVKQEGEYEPEEHPEPDTDYIRAQEARRFMIYVHTKMMIVDDEYIIIGSANINQRSMDGARDSEIAMGAYQPYHLATRQPARGQIHGFRMSLWYEHLGMLEDVFQRPESVECVQKVNEVAEKYWDLYSSDDLEQDLPGHLLSYPIGVTADGSVTELPGMENFP 792

HSP 2 Score: 42.3578 bits (98), Expect = 5.354e-87
Identity = 17/21 (80.95%), Postives = 20/21 (95.24%), Query Frame = 1 
Query:  514 PDTKARVLGTKSDYMPPVLTT 576
            PDT+ARVLG KSDY+PP+LTT
Sbjct:  792 PDTRARVLGNKSDYLPPILTT 812
[Close]
BLAST of EY746125 vs. ExPASy Swiss-Prot
Match: PLDA1_ORYSJ (Phospholipase D alpha 1 OS=Oryza sativa subsp. japonica GN=PLD1 PE=1 SV=2)

HSP 1 Score: 300.442 bits (768), Expect = 9.111e-87
Identity = 138/171 (80.70%), Postives = 157/171 (91.81%), Query Frame = 3 
Query:    3 TFFCLGNREVKRSGEYEPAERPEDDSDYLRAQEARRFMIYVHAKMMIVDDEYIIIGSANINQRSMEGARDTEIAMGGYQPYHLSTRVPARGQIHGFRMSLWYEHLGMLDDTFLQPESSECVKKVNQIAERYWDLYSSESLEHDLPGHLLRYPIGVSGEGDVTELPGTEFFP 515
            TFFCLGNREVK++GEY+P E+PE D+DY RAQEARRFMIYVH KMMIVDDEYIIIGSANINQRSM+GARD+EIAMGGYQPYHL+TR PARGQIHGFRM+LWYEHLGMLDD F +PES ECV+KVN+IAE+YWD+YSS+ L+ DLPGHLL YPIGV+ +G VTELPG E+FP
Sbjct:  622 TFFCLGNREVKQAGEYQPEEQPEADTDYSRAQEARRFMIYVHTKMMIVDDEYIIIGSANINQRSMDGARDSEIAMGGYQPYHLATRQPARGQIHGFRMALWYEHLGMLDDVFQRPESLECVQKVNRIAEKYWDMYSSDDLQQDLPGHLLSYPIGVASDGVVTELPGMEYFP 792

HSP 2 Score: 41.9726 bits (97), Expect = 9.111e-87
Identity = 17/21 (80.95%), Postives = 20/21 (95.24%), Query Frame = 1 
Query:  514 PDTKARVLGTKSDYMPPVLTT 576
            PDT+ARVLG KSDYMPP+LT+
Sbjct:  792 PDTRARVLGAKSDYMPPILTS 812
The following BLAST results are available for this feature:
BLAST of EY746125 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 19
Match NameE-valueIdentityDescription
PLDA1_RICCO1.678e-9487.72Phospholipase D alpha 1 OS=Ricinus communis GN=PLD... [more]
PLDA2_ARATH4.076e-9384.21Phospholipase D alpha 2 OS=Arabidopsis thaliana GN... [more]
PLDA1_ARATH1.292e-9183.63Phospholipase D alpha 1 OS=Arabidopsis thaliana GN... [more]
PLDA1_BRAOC6.366e-9183.63Phospholipase D alpha 1 OS=Brassica oleracea var. ... [more]
PLDA1_TOBAC1.413e-9083.04Phospholipase D alpha 1 OS=Nicotiana tabacum GN=PL... [more]
PLDA1_VIGUN5.338e-9084.80Phospholipase D alpha 1 OS=Vigna unguiculata GN=PL... [more]
PLDA2_BRAOC4.476e-8982.46Phospholipase D alpha 2 OS=Brassica oleracea var. ... [more]
PLDA1_PIMBR4.896e-8881.87Phospholipase D alpha 1 OS=Pimpinella brachycarpa ... [more]
PLDA1_MAIZE5.354e-8781.29Phospholipase D alpha 1 OS=Zea mays GN=PLD1 PE=2 S... [more]
PLDA1_ORYSJ9.111e-8780.70Phospholipase D alpha 1 OS=Oryza sativa subsp. jap... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C5-003-028-C08-CT.F Sweet orange flower, greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY746125 ID=EY746125; Name=EY746125; organism=Citrus sinensis; type=EST; length=807bp
TGACATTCTTCTGCCTTGGGAATCGGGAGGTGAAGAGGAGTGGTGAATAT
GAACCTGCAGAAAGGCCGGAGGATGATTCAGACTATCTTAGAGCTCAGGA
AGCTCGACGCTTCATGATCTACGTTCATGCCAAGATGATGATTGTTGATG
ACGAATACATTATAATTGGATCTGCCAACATCAACCAGCGATCAATGGAG
GGTGCCAGGGACACTGAGATAGCCATGGGAGGCTACCAACCATATCATTT
GTCAACCAGGGTGCCAGCACGAGGCCAGATACACGGCTTCCGTATGTCAC
TATGGTACGAGCACCTAGGCATGCTCGATGACACCTTCCTCCAGCCAGAA
AGTAGCGAATGTGTTAAGAAGGTTAACCAGATTGCCGAACGGTATTGGGA
CCTGTACTCCAGTGAGTCTCTTGAGCATGACTTGCCCGGTCACTTGCTCC
GCTACCCCATTGGTGTTTCTGGTGAAGGAGATGTCACGGAGTTGCCAGGA
ACCGAGTTCTTCCCCCGACACCAAGGCTCGTGTTCTCGGTACCAAATCGG
ATTATATGCCCCCAGTCCTGACCACCTGATGCTTCTCCAGAGGGTGCCCA
AAATAATTTCGTAGTTGCGAACTACCGGGTTATGGTACTATAACCAATTA
TTAAATGATGTGTATTAAAACCAACCAGGGGGGGTATTGGTTTTCCGGCT
TGGGTTCAAAAAATGTGGGGTGATCTTGGGGATATCCTGACCGGGGAACA
CTTCTTAAGTCCGGTTTTTTTGGGGGCATTACCGACAAACTTGGGCCCCA
AATTTTG
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