EY710657

Overview
NameEY710657
Unique NameEY710657
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length1067
Libraries
Library NameType
Sweet orange fruit, development stadium (2 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY710657 vs. ExPASy Swiss-Prot
Match: SSG1_MANES (Granule-bound starch synthase 1, chloroplastic/amyloplastic OS=Manihot esculenta GN=WAXY PE=2 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.717e-19
Identity = 51/129 (39.53%), Postives = 67/129 (51.94%), Query Frame = 2 
Query:   92 MATAVAASSFVSRSYGHEVASGLNSKASQMKF-----RCMKHSGLRSLNIIDELQVKTMASKLATSRQAGRNGFRSQNGPSLMIVCGVGLNILFVGTEVAPWSKTXXXXXXXXXXXXXXXXXXHRVMTI 463
            MAT +AA  FVSRS  H     L +KA+ +       + +  +GLRSLN +D+LQ+KT  SK      A  NG      P+  I+CG G+N++FVG EV PWSKT                  HRVMT+
Sbjct:    1 MATVIAAH-FVSRS-SHLSIHALETKANNLSHTGPWTQTITPNGLRSLNTMDKLQMKTQ-SKAVKKVSATGNG-----RPAAKIICGHGMNLIFVGAEVGPWSKTGGLGDVLGGLPPAMAARGHRVMTV 121

HSP 2 Score: 40.817 bits (94), Expect = 1.717e-19
Identity = 20/44 (45.45%), Postives = 27/44 (61.36%), Query Frame = 1 
Query:  469 PDMTSTKMAWDTDVVIELKVGEQIEKVRFLYYAQEKKFKRGVKR 600
            P     K AWDT V +E+K+G++IE VRF +      +KRGV R
Sbjct:  123 PRYDQYKDAWDTSVSVEIKIGDRIETVRFFH-----SYKRGVDR 161

HSP 3 Score: 23.8682 bits (50), Expect = 1.717e-19
Identity = 8/10 (80.00%), Postives = 10/10 (100.00%), Query Frame = 3 
Query:  462 LAPRYDQYKD 491
            ++PRYDQYKD
Sbjct:  121 VSPRYDQYKD 130
[Close]
BLAST of EY710657 vs. ExPASy Swiss-Prot
Match: SSG1_SOLTU (Granule-bound starch synthase 1, chloroplastic/amyloplastic OS=Solanum tuberosum GN=WAXY PE=1 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 4.839e-19
Identity = 49/126 (38.89%), Postives = 66/126 (52.38%), Query Frame = 2 
Query:   92 MATAVAASSFVSRSYGHEVASGLNSKASQMKFR--CMKHSGLRSLNIIDELQVKTMASKLATSRQAGRNGFRSQNGPSLMIVCGVGLNILFVGTEVAPWSKTXXXXXXXXXXXXXXXXXXHRVMTI 463
            MA+  A+  FVSRS   + +    S  SQ+  R   + H+GLR++N +D LQ +T      T + A R   + + G S  IVCG G+N++FVGTEV PWSKT                  HRVMTI
Sbjct:    1 MASITASHHFVSRS---QTSLDTKSTLSQIGLRNHTLTHNGLRAVNKLDGLQSRTNTK--VTPKMASRTETK-RPGCSATIVCGKGMNLIFVGTEVGPWSKTGGLGDVLGGLPPALAARGHRVMTI 120

HSP 2 Score: 39.6614 bits (91), Expect = 4.839e-19
Identity = 21/44 (47.73%), Postives = 26/44 (59.09%), Query Frame = 1 
Query:  469 PDMTSTKMAWDTDVVIELKVGEQIEKVRFLYYAQEKKFKRGVKR 600
            P     K AWDT V +E+KVG+ IE VRF +      +KRGV R
Sbjct:  122 PRYDQYKDAWDTSVAVEVKVGDSIEIVRFFHC-----YKRGVDR 160

HSP 3 Score: 24.2534 bits (51), Expect = 4.839e-19
Identity = 8/10 (80.00%), Postives = 10/10 (100.00%), Query Frame = 3 
Query:  462 LAPRYDQYKD 491
            ++PRYDQYKD
Sbjct:  120 ISPRYDQYKD 129
[Close]
BLAST of EY710657 vs. ExPASy Swiss-Prot
Match: SSG1_ARATH (Probable granule-bound starch synthase 1, chloroplastic/amyloplastic OS=Arabidopsis thaliana GN=WAXY PE=2 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 3.836e-18
Identity = 45/127 (35.43%), Postives = 68/127 (53.54%), Query Frame = 2 
Query:   92 MATAVAASSFVSRS--YGHEVASGLNSKAS-QMKFRCMKHSGLRSLNIIDELQVKTMASKLATSRQAGRNGFRSQNGPSLMIVCGVGLNILFVGTEVAPWSKTXXXXXXXXXXXXXXXXXXHRVMTI 463
            MAT  A+S+FVSR+  + +  AS  +  A   +K + + H GLRS N++D LQ ++ A K  +++ + R+      G    IVC  G++++F+G EV PWSKT                  HRVMTI
Sbjct:    1 MATVTASSNFVSRTSLFNNHGASSCSDVAQITLKGQSLTHCGLRSFNMVDNLQRRSQA-KPVSAKSSKRSSKVKTAGK---IVCEKGMSVIFIGAEVGPWSKTGGLGDVLGGLPPALAARGHRVMTI 123

HSP 2 Score: 40.4318 bits (93), Expect = 3.836e-18
Identity = 20/44 (45.45%), Postives = 28/44 (63.64%), Query Frame = 1 
Query:  469 PDMTSTKMAWDTDVVIELKVGEQIEKVRFLYYAQEKKFKRGVKR 600
            P     K AWDT VV+++KVG+++E VRF +      +KRGV R
Sbjct:  125 PRYDQYKDAWDTCVVVQIKVGDKVENVRFFHC-----YKRGVDR 163

HSP 3 Score: 23.8682 bits (50), Expect = 3.836e-18
Identity = 8/10 (80.00%), Postives = 9/10 (90.00%), Query Frame = 3 
Query:  462 LAPRYDQYKD 491
            + PRYDQYKD
Sbjct:  123 ICPRYDQYKD 132
[Close]
BLAST of EY710657 vs. ExPASy Swiss-Prot
Match: SSG1_ANTMA (Granule-bound starch synthase 1, chloroplastic/amyloplastic OS=Antirrhinum majus GN=WAXY PE=2 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 1.398e-17
Identity = 44/127 (34.65%), Postives = 65/127 (51.18%), Query Frame = 2 
Query:   92 MATAVAASSFVSRSYGHEVAS-GLNSKASQMKFRCMK--HSGLRSLNIIDELQVKTMASKLATSRQAGRNGFRSQNGPSLMIVCGVGLNILFVGTEVAPWSKTXXXXXXXXXXXXXXXXXXHRVMTI 463
            MAT V AS  VS  +G   +S    +  +Q+  R  +  H+GLRS+N++D+LQ++  A +  +  +   NG      P   I+CG G+N++FV  EV PWSKT                  HRVMT+
Sbjct:    1 MAT-VTASQLVSHVHGGATSSPDTKTNLAQVGLRNQQFTHNGLRSINMVDKLQMRNNAKQSRSLVKKTDNG-----SPLGKIICGTGMNLVFVLAEVGPWSKTGGLGDVVGGLPPAMAGNGHRVMTV 121

HSP 2 Score: 42.3578 bits (98), Expect = 1.398e-17
Identity = 22/44 (50.00%), Postives = 27/44 (61.36%), Query Frame = 1 
Query:  469 PDMTSTKMAWDTDVVIELKVGEQIEKVRFLYYAQEKKFKRGVKR 600
            P     K AWDT VV+E+KVG+ IE VRF +      +KRGV R
Sbjct:  123 PRYDQYKDAWDTSVVVEIKVGDSIETVRFFHC-----YKRGVDR 161

HSP 3 Score: 23.8682 bits (50), Expect = 1.398e-17
Identity = 8/10 (80.00%), Postives = 10/10 (100.00%), Query Frame = 3 
Query:  462 LAPRYDQYKD 491
            ++PRYDQYKD
Sbjct:  121 VSPRYDQYKD 130
[Close]
BLAST of EY710657 vs. ExPASy Swiss-Prot
Match: SSG1_IPOBA (Granule-bound starch synthase 1, chloroplastic/amyloplastic OS=Ipomoea batatas GN=WAXY PE=2 SV=2)

HSP 1 Score: 59.6918 bits (143), Expect = 8.703e-16
Identity = 45/128 (35.16%), Postives = 61/128 (47.66%), Query Frame = 2 
Query:   92 MATAVAASSFVSRSYGHEVASGLNSKASQMKFR--CMKHSGLRSLNIIDELQVKTMASKLATSRQAGRNGFRSQNGPSL-MIVCGV-GLNILFVGTEVAPWSKTXXXXXXXXXXXXXXXXXXHRVMTI 463
            MAT + AS FVS   G   +        Q+  R   + H+GLR +N ID LQ++T A K        +NG  ++ G +   IVC   G+N++FVG EV PW KT                  HRVMT+
Sbjct:    1 MAT-ITASHFVSHVCGGATSGESKVGLGQLALRSQAVTHNGLRPVNKIDMLQLRTSAKK------PSKNGRENEGGMAAGTIVCKQQGMNLVFVGCEVGPWCKTGGLGDVLGGLPPALAARGHRVMTV 121

HSP 2 Score: 41.5874 bits (96), Expect = 8.703e-16
Identity = 22/44 (50.00%), Postives = 28/44 (63.64%), Query Frame = 1 
Query:  469 PDMTSTKMAWDTDVVIELKVGEQIEKVRFLYYAQEKKFKRGVKR 600
            P     K AWDT VV+EL+VG++IE VRF +      +KRGV R
Sbjct:  123 PRYDQYKDAWDTCVVVELQVGDRIEPVRFFH-----SYKRGVDR 161

HSP 3 Score: 23.483 bits (49), Expect = 8.703e-16
Identity = 8/10 (80.00%), Postives = 9/10 (90.00%), Query Frame = 3 
Query:  462 LAPRYDQYKD 491
            + PRYDQYKD
Sbjct:  121 VCPRYDQYKD 130
[Close]
BLAST of EY710657 vs. ExPASy Swiss-Prot
Match: SSG1_SORBI (Granule-bound starch synthase 1, chloroplastic/amyloplastic OS=Sorghum bicolor GN=WAXY PE=2 SV=1)

HSP 1 Score: 53.1434 bits (126), Expect = 1.511e-12
Identity = 27/78 (34.62%), Postives = 37/78 (47.44%), Query Frame = 2 
Query:  230 DELQVKTMASKLATSRQAGRNGFRSQNGPSLMIVCGVGLNILFVGTEVAPWSKTXXXXXXXXXXXXXXXXXXHRVMTI 463
            D L ++T A      + A R G R    PSL++    G+N++FVG E+APWSKT                  HRVM +
Sbjct:   44 DALSMRTSACPAPRQQPAARRGGRGGRFPSLVVCATAGMNVVFVGAEMAPWSKTGGLGDVLGGLPPAMAANGHRVMVV 121

HSP 2 Score: 36.5798 bits (83), Expect = 1.511e-12
Identity = 20/44 (45.45%), Postives = 25/44 (56.82%), Query Frame = 1 
Query:  469 PDMTSTKMAWDTDVVIELKVGEQIEKVRFLYYAQEKKFKRGVKR 600
            P     K AWDT VV E+K+G+  E VRF +      +KRGV R
Sbjct:  123 PRYDQYKDAWDTSVVSEIKMGDGYETVRFFHC-----YKRGVDR 161

HSP 3 Score: 23.8682 bits (50), Expect = 1.511e-12
Identity = 8/10 (80.00%), Postives = 10/10 (100.00%), Query Frame = 3 
Query:  462 LAPRYDQYKD 491
            ++PRYDQYKD
Sbjct:  121 VSPRYDQYKD 130
[Close]
BLAST of EY710657 vs. ExPASy Swiss-Prot
Match: SSG1_MAIZE (Granule-bound starch synthase 1, chloroplastic/amyloplastic OS=Zea mays GN=WAXY PE=3 SV=1)

HSP 1 Score: 51.6026 bits (122), Expect = 4.214e-12
Identity = 29/86 (33.72%), Postives = 40/86 (46.51%), Query Frame = 2 
Query:  206 GLRSLNIIDELQVKTMASKLATSRQAGRNGFRSQNGPSLMIVCGVGLNILFVGTEVAPWSKTXXXXXXXXXXXXXXXXXXHRVMTI 463
            G R+    D L ++T A      +Q  R G R    PSL++    G+N++FVG E+APWSKT                  HRVM +
Sbjct:   34 GARASAAADTLSMRTSARAAPRHQQQARRGGRF---PSLVVCASAGMNVVFVGAEMAPWSKTGGLGDVLGGLPPAMAANGHRVMVV 116

HSP 2 Score: 36.5798 bits (83), Expect = 4.214e-12
Identity = 20/44 (45.45%), Postives = 25/44 (56.82%), Query Frame = 1 
Query:  469 PDMTSTKMAWDTDVVIELKVGEQIEKVRFLYYAQEKKFKRGVKR 600
            P     K AWDT VV E+K+G+  E VRF +      +KRGV R
Sbjct:  118 PRYDQYKDAWDTSVVSEIKMGDGYETVRFFHC-----YKRGVDR 156

HSP 3 Score: 23.8682 bits (50), Expect = 4.214e-12
Identity = 8/10 (80.00%), Postives = 10/10 (100.00%), Query Frame = 3 
Query:  462 LAPRYDQYKD 491
            ++PRYDQYKD
Sbjct:  116 VSPRYDQYKD 125
The following BLAST results are available for this feature:
BLAST of EY710657 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 7
Match NameE-valueIdentityDescription
SSG1_MANES1.717e-1939.53Granule-bound starch synthase 1, chloroplastic/amy... [more]
SSG1_SOLTU4.839e-1938.89Granule-bound starch synthase 1, chloroplastic/amy... [more]
SSG1_ARATH3.836e-1835.43Probable granule-bound starch synthase 1, chloropl... [more]
SSG1_ANTMA1.398e-1734.65Granule-bound starch synthase 1, chloroplastic/amy... [more]
SSG1_IPOBA8.703e-1635.16Granule-bound starch synthase 1, chloroplastic/amy... [more]
SSG1_SORBI1.511e-1234.62Granule-bound starch synthase 1, chloroplastic/amy... [more]
SSG1_MAIZE4.214e-1233.72Granule-bound starch synthase 1, chloroplastic/amy... [more]
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Properties
Property NameValue
Genbank descriptionCS00-C3-701-083-F12-CT.F Sweet orange fruit, development stadium (2 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY710657 ID=EY710657; Name=EY710657; organism=Citrus sinensis; type=EST; length=1067bp
CCACGCGTCCGCGCTTGAGCACCAGATTTCATCACGAAACTAAGAGGGAA
ACAAATCTTCAGCAGAGACTACAAACATAATAATATCAGCTATGGCTACC
GCTGTAGCCGCTTCAAGCTTCGTCTCAAGGAGTTATGGACATGAGGTAGC
TTCAGGATTGAATAGTAAGGCATCACAGATGAAATTTAGGTGTATGAAGC
ACAGTGGGCTGAGAAGTTTAAATATCATAGACGAACTCCAAGTGAAAACA
ATGGCATCAAAACTAGCCACCAGCAGGCAAGCTGGGAGGAATGGATTTAG
AAGCCAGAACGGGCCATCCTTAATGATTGTTTGTGGGGTTGGCCTGAATA
TCCTCTTTGTTGGAACAGAGGTTGCTCCTTGGAGTAAAACTGGGGGACTT
GGTGATGTTCTTGGAGGTCTGCCACCAGCACTGGCCGCCAATGGGCACCG
TGTCATGACAATTGGCACCCCGATATGACCAGTACAAAGATGGCTTGGGA
TACTGATGTTGTAATTGAGCTGAAAGTTGGAGAACAAATTGAAAAAGTTC
GCTTTCTTTACTATGCCCAAGAAAAGAAGTTTAAAAGGGGTGTTAAGAGG
AAAACCCTAGGGTACCTGAGAAAAGGGGAAGGGGGAAACGAACAGTAACA
AAAAAAGGAGGCCACAGAAGGGGAGAGGACCACCAGGAGAAAGCGACAGA
GCTGATGGAGAAGGAGAAGGACCGGACAAAAGCAGGAGCAAACAAGACAA
AGGAAGAAAGGAGACAGACAAAAAAAAAAATCATAAAGACGAAACCAATA
AGAACAAAATGGGACGACGAGAAACCGAGAGGAGACGGGCGGAGAGAACC
ACACACACAGAGGAAGAAGCACACAAAGAAGGAAACAGGAGCACACACGG
CGACAGAAGCGAGAAAGAAACCACACGAAAGCGAGGCAAGAAAAGAAAGA
GAGGAAGAGCGAGAGCGAAGAAACAAAGAGCACACACAAAACAAAAACGA
CAGGGACCAGAAAACGACGAAGAAGAAAAAGNCACAGTGAGGAACAGACA
AAGACAGAGACAGAAAA
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