EY710539 | Citrus Genome Database

Overview


Name	EY710539
Unique Name	EY710539
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	999

Libraries


Library Name	Type
Sweet orange fruit, development stadium (2 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

BLAST of EY710539 vs. ExPASy Swiss-Prot
Match: PSMD2_DICDI (26S proteasome non-ATPase regulatory subunit 2 OS=Dictyostelium discoideum GN=psmD2 PE=2 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 2.013e-38
Identity = 71/121 (58.68%), Postives = 92/121 (76.03%), Query Frame = 3

Query:   45 MTLLSCAYAGTGNVLKVQNLLGHCAQHHEKGEAYQGPAVLGIAMVAMAEELGLEMAIRSLEHLLQYGEQNIRRAVPLALGLLCISNPKVNVMDTLSRLSHDTDSEVAMAAVISLGLIGSGT 407
            +T+ +CAYAGTGNVLKVQN+L  C+   E    + G AVL IA++AM EELG +M +R  +HLLQ G  +I+RA+PLALGLL  SNP++ +MD LS+LSHD D EVA  A++SLGLIG+GT
Sbjct:  595 LTVEACAYAGTGNVLKVQNMLHFCSDGQEN--PHHGLAVLSIALIAMGEELGSDMCLRMFDHLLQKGNVHIKRAIPLALGLLSPSNPRIAIMDILSKLSHDNDPEVAQGAILSLGLIGAGT 713

HSP 2 Score: 31.5722 bits (70), Expect = 2.013e-38
Identity = 19/38 (50.00%), Postives = 24/38 (63.16%), Query Frame = 2

Query:  461 KDANLLFCVPIAQGLVHMGKGLLDPLTQTFLDRFLASP 574
            KD +L F V IAQGL+H+GKG +  +     DR L SP
Sbjct:  731 KDVHLFF-VRIAQGLLHLGKGTM-TINPYHSDRTLMSP 766

HSP 3 Score: 27.7202 bits (60), Expect = 2.013e-38
Identity = 13/22 (59.09%), Postives = 15/22 (68.18%), Query Frame = 1

Query:  397 GRERNNARIAGMLRNLSSYYYK 462
            G   NNARI GMLR L+ +Y K
Sbjct:  710 GAGTNNARIGGMLRALAVFYGK 731

BLAST of EY710539 vs. ExPASy Swiss-Prot
Match: PSMD2_RAT (26S proteasome non-ATPase regulatory subunit 2 OS=Rattus norvegicus GN=Psmd2 PE=2 SV=1)

HSP 1 Score: 140.198 bits (352), Expect = 3.774e-36
Identity = 69/139 (49.64%), Postives = 95/139 (68.35%), Query Frame = 3

Query:   60 CAYAGTGNVLKVQNLLGHCAQHHEKGE-----------------------AYQGPAVLGIAMVAMAEELGLEMAIRSLEHLLQYGEQNIRRAVPLALGLLCISNPKVNVMDTLSRLSHDTDSEVAMAAVISLGLIGSGT 407
            CAYAG+GNVLKVQ LL  C++H +  E                       A+QG AVLGIA++AM EE+G EMA+R+  HLL+YGE  +RRAVPLAL L+ +SNP++N++DTLS+ SHD D EV+  ++ ++G++GSGT
Sbjct:  598 CAYAGSGNVLKVQQLLHICSEHFDSKEKEEDKDKKEKKDKDKKEAPADMGAHQGVAVLGIALIAMGEEIGAEMALRTFGHLLRYGEPTLRRAVPLALALISVSNPRLNILDTLSKFSHDADPEVSYNSIFAMGMVGSGT 736

HSP 2 Score: 33.4982 bits (75), Expect = 3.774e-36
Identity = 15/23 (65.22%), Postives = 17/23 (73.91%), Query Frame = 2

Query:  461 KDANLLFCVPIAQGLVHMGKGLL 529
            KD N LF V +AQGL H+GKG L
Sbjct:  754 KDPNNLFMVRLAQGLTHLGKGTL 776

BLAST of EY710539 vs. ExPASy Swiss-Prot
Match: PSMD2_PONAB (26S proteasome non-ATPase regulatory subunit 2 OS=Pongo abelii GN=PSMD2 PE=2 SV=1)

HSP 1 Score: 140.198 bits (352), Expect = 3.774e-36
Identity = 69/139 (49.64%), Postives = 95/139 (68.35%), Query Frame = 3

Query:   60 CAYAGTGNVLKVQNLLGHCAQHHEKGE-----------------------AYQGPAVLGIAMVAMAEELGLEMAIRSLEHLLQYGEQNIRRAVPLALGLLCISNPKVNVMDTLSRLSHDTDSEVAMAAVISLGLIGSGT 407
            CAYAG+GNVLKVQ LL  C++H +  E                       A+QG AVLGIA++AM EE+G EMA+R+  HLL+YGE  +RRAVPLAL L+ +SNP++N++DTLS+ SHD D EV+  ++ ++G++GSGT
Sbjct:  598 CAYAGSGNVLKVQQLLHICSEHFDSKEKEEDKDKKEKKDKDKKEAPADMGAHQGVAVLGIALIAMGEEIGAEMALRTFGHLLRYGEPTLRRAVPLALALISVSNPRLNILDTLSKFSHDADPEVSYNSIFAMGMVGSGT 736

HSP 2 Score: 33.4982 bits (75), Expect = 3.774e-36
Identity = 15/23 (65.22%), Postives = 17/23 (73.91%), Query Frame = 2

Query:  461 KDANLLFCVPIAQGLVHMGKGLL 529
            KD N LF V +AQGL H+GKG L
Sbjct:  754 KDPNNLFMVRLAQGLTHLGKGTL 776

BLAST of EY710539 vs. ExPASy Swiss-Prot
Match: PSMD2_MOUSE (26S proteasome non-ATPase regulatory subunit 2 OS=Mus musculus GN=Psmd2 PE=1 SV=1)

HSP 1 Score: 140.198 bits (352), Expect = 3.774e-36
Identity = 69/139 (49.64%), Postives = 95/139 (68.35%), Query Frame = 3

Query:   60 CAYAGTGNVLKVQNLLGHCAQHHEKGE-----------------------AYQGPAVLGIAMVAMAEELGLEMAIRSLEHLLQYGEQNIRRAVPLALGLLCISNPKVNVMDTLSRLSHDTDSEVAMAAVISLGLIGSGT 407
            CAYAG+GNVLKVQ LL  C++H +  E                       A+QG AVLGIA++AM EE+G EMA+R+  HLL+YGE  +RRAVPLAL L+ +SNP++N++DTLS+ SHD D EV+  ++ ++G++GSGT
Sbjct:  598 CAYAGSGNVLKVQQLLHICSEHFDSKEKEEDKDKKEKKDKDKKEAPADMGAHQGVAVLGIALIAMGEEIGAEMALRTFGHLLRYGEPTLRRAVPLALALISVSNPRLNILDTLSKFSHDADPEVSYNSIFAMGMVGSGT 736

HSP 2 Score: 33.4982 bits (75), Expect = 3.774e-36
Identity = 15/23 (65.22%), Postives = 17/23 (73.91%), Query Frame = 2

Query:  461 KDANLLFCVPIAQGLVHMGKGLL 529
            KD N LF V +AQGL H+GKG L
Sbjct:  754 KDPNNLFMVRLAQGLTHLGKGTL 776

BLAST of EY710539 vs. ExPASy Swiss-Prot
Match: PSMD2_HUMAN (26S proteasome non-ATPase regulatory subunit 2 OS=Homo sapiens GN=PSMD2 PE=1 SV=3)

HSP 1 Score: 140.198 bits (352), Expect = 3.774e-36
Identity = 69/139 (49.64%), Postives = 95/139 (68.35%), Query Frame = 3

Query:   60 CAYAGTGNVLKVQNLLGHCAQHHEKGE-----------------------AYQGPAVLGIAMVAMAEELGLEMAIRSLEHLLQYGEQNIRRAVPLALGLLCISNPKVNVMDTLSRLSHDTDSEVAMAAVISLGLIGSGT 407
            CAYAG+GNVLKVQ LL  C++H +  E                       A+QG AVLGIA++AM EE+G EMA+R+  HLL+YGE  +RRAVPLAL L+ +SNP++N++DTLS+ SHD D EV+  ++ ++G++GSGT
Sbjct:  598 CAYAGSGNVLKVQQLLHICSEHFDSKEKEEDKDKKEKKDKDKKEAPADMGAHQGVAVLGIALIAMGEEIGAEMALRTFGHLLRYGEPTLRRAVPLALALISVSNPRLNILDTLSKFSHDADPEVSYNSIFAMGMVGSGT 736

HSP 2 Score: 33.4982 bits (75), Expect = 3.774e-36
Identity = 15/23 (65.22%), Postives = 17/23 (73.91%), Query Frame = 2

Query:  461 KDANLLFCVPIAQGLVHMGKGLL 529
            KD N LF V +AQGL H+GKG L
Sbjct:  754 KDPNNLFMVRLAQGLTHLGKGTL 776

BLAST of EY710539 vs. ExPASy Swiss-Prot
Match: PSMD2_BOVIN (26S proteasome non-ATPase regulatory subunit 2 OS=Bos taurus GN=PSMD2 PE=1 SV=2)

HSP 1 Score: 140.198 bits (352), Expect = 3.774e-36
Identity = 69/139 (49.64%), Postives = 95/139 (68.35%), Query Frame = 3

Query:   60 CAYAGTGNVLKVQNLLGHCAQHHEKGE-----------------------AYQGPAVLGIAMVAMAEELGLEMAIRSLEHLLQYGEQNIRRAVPLALGLLCISNPKVNVMDTLSRLSHDTDSEVAMAAVISLGLIGSGT 407
            CAYAG+GNVLKVQ LL  C++H +  E                       A+QG AVLGIA++AM EE+G EMA+R+  HLL+YGE  +RRAVPLAL L+ +SNP++N++DTLS+ SHD D EV+  ++ ++G++GSGT
Sbjct:  598 CAYAGSGNVLKVQQLLHICSEHFDSKEKEEDKDKKEKKDKDKKEAPADMGAHQGVAVLGIALIAMGEEIGAEMALRTFGHLLRYGEPTLRRAVPLALALISVSNPRLNILDTLSKFSHDADPEVSYNSIFAMGMVGSGT 736

HSP 2 Score: 33.4982 bits (75), Expect = 3.774e-36
Identity = 15/23 (65.22%), Postives = 17/23 (73.91%), Query Frame = 2

Query:  461 KDANLLFCVPIAQGLVHMGKGLL 529
            KD N LF V +AQGL H+GKG L
Sbjct:  754 KDPNNLFMVRLAQGLTHLGKGTL 776

BLAST of EY710539 vs. ExPASy Swiss-Prot
Match: RPN1_NEUCR (26S proteasome regulatory subunit rpn-1 OS=Neurospora crassa GN=rpn-1 PE=3 SV=2)

HSP 1 Score: 135.961 bits (341), Expect = 1.172e-34
Identity = 68/122 (55.74%), Postives = 89/122 (72.95%), Query Frame = 3

Query:   60 CAYAGTGNVLKVQNLLGHCAQHHEKGEAYQGP------AVLGIAMVAMAEELGLEMAIRSLEHLLQYGEQNIRRAVPLALGLLCISNPKVNVMDTLSRLSHDTDSEVAMAAVISLGLIGSGT 407
            CA+AGTG VLK+Q LL  C +H E GE  +G       AVLGI ++AM E++G EM +R   HL+ YGE NIRRAVPLA+GL+  SNP++ V DTLSR SHD D+EVA+ A+ ++GL+G+GT
Sbjct:  607 CAWAGTGAVLKIQELLHICNEHIEDGEEKKGEELLQAYAVLGIGLIAMGEDVGQEMVLRHFGHLMHYGEANIRRAVPLAMGLISPSNPQMKVYDTLSRYSHDNDNEVAINAIFAMGLLGAGT 728

HSP 2 Score: 32.7278 bits (73), Expect = 1.172e-34
Identity = 15/23 (65.22%), Postives = 17/23 (73.91%), Query Frame = 2

Query:  461 KDANLLFCVPIAQGLVHMGKGLL 529
            +D   LF V IAQGL+HMGKG L
Sbjct:  746 RDQESLFMVRIAQGLLHMGKGTL 768

BLAST of EY710539 vs. ExPASy Swiss-Prot
Match: RPN1_SCHPO (26S proteasome regulatory subunit rpn1 OS=Schizosaccharomyces pombe GN=rpn1 PE=1 SV=2)

HSP 1 Score: 125.176 bits (313), Expect = 4.219e-31
Identity = 60/121 (49.59%), Postives = 84/121 (69.42%), Query Frame = 3

Query:   60 CAYAGTGNVLKVQNLLGHCAQH-----HEKGEAYQGPAVLGIAMVAMAEELGLEMAIRSLEHLLQYGEQNIRRAVPLALGLLCISNPKVNVMDTLSRLSHDTDSEVAMAAVISLGLIGSGT 407
            C+YAGTGNVL +Q LL  C++       E     Q  A LG+A +AM E++G EM +R  +H++ YGE +IR+A+PLALGLL  SNP++ + DTLSR SHD D +VA  A+ ++GL+G+GT
Sbjct:  597 CSYAGTGNVLHIQKLLHICSEPPSDDAKESETTIQTFAALGVATIAMGEDIGAEMVLRHFDHMMHYGEPSIRKAIPLALGLLSASNPQMRIFDTLSRYSHDNDLDVAYNAIFAMGLVGAGT 717

HSP 2 Score: 31.5722 bits (70), Expect = 4.219e-31
Identity = 13/23 (56.52%), Postives = 19/23 (82.61%), Query Frame = 2

Query:  461 KDANLLFCVPIAQGLVHMGKGLL 529
            K++N LF V IAQGL+++GKG +
Sbjct:  735 KESNALFMVRIAQGLLYLGKGTM 757

BLAST of EY710539 vs. ExPASy Swiss-Prot
Match: RPN1_YEAST (26S proteasome regulatory subunit RPN1 OS=Saccharomyces cerevisiae GN=RPN1 PE=1 SV=3)

HSP 1 Score: 100.138 bits (248), Expect = 7.378e-23
Identity = 43/84 (51.19%), Postives = 67/84 (79.76%), Query Frame = 3

Query:  156 AVLGIAMVAMAEELGLEMAIRSLEHLLQYGEQNIRRAVPLALGLLCISNPKVNVMDTLSRLSHDTDSEVAMAAVISLGLIGSGT 407
            AVLGIA++A+ E++G EM++R   HL+ YG ++IRR VPLA+G++ +S+P++ V DTL+R SHD D EV+M ++ ++GL G+GT
Sbjct:  739 AVLGIALIALGEDIGKEMSLRHFGHLMHYGNEHIRRMVPLAMGIVSVSDPQMKVFDTLTRFSHDADLEVSMNSIFAMGLCGAGT 822

HSP 2 Score: 28.8758 bits (63), Expect = 7.378e-23
Identity = 15/43 (34.88%), Postives = 23/43 (53.49%), Query Frame = 1

Query:  397 GRERNNARIAGMLRNLSSYYYKRCQSSVLCPNCSRISAHGKGS 525
            G   NNAR+A +LR L+SYY +   +  +      +   GKG+
Sbjct:  819 GAGTNNARLAQLLRQLASYYSREQDALFITRLAQGLLHLGKGT 861

BLAST of EY710539 vs. ExPASy Swiss-Prot
Match: RPN1_CANGA (26S proteasome regulatory subunit RPN1 OS=Candida glabrata GN=RPN1 PE=3 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 4.655e-22
Identity = 42/84 (50.00%), Postives = 65/84 (77.38%), Query Frame = 3

Query:  156 AVLGIAMVAMAEELGLEMAIRSLEHLLQYGEQNIRRAVPLALGLLCISNPKVNVMDTLSRLSHDTDSEVAMAAVISLGLIGSGT 407
            AV+GIA++ M EE+G EM++R   HL+ YG ++IRR VPLA+GL+ +++P++ V DTL+R SHD D +V+M ++ ++GL G GT
Sbjct:  729 AVIGIALITMGEEIGKEMSLRHFGHLMHYGNEHIRRMVPLAMGLVSVADPQMKVFDTLTRFSHDPDLDVSMNSIFAMGLCGVGT 812

HSP 2 Score: 29.261 bits (64), Expect = 4.655e-22
Identity = 11/24 (45.83%), Postives = 18/24 (75.00%), Query Frame = 2

Query:  458 TKDANLLFCVPIAQGLVHMGKGLL 529
            +++ + LF   +AQGLVH+GKG +
Sbjct:  829 SREQDALFITRLAQGLVHLGKGTM 852

The following BLAST results are available for this feature:

BLAST of EY710539 vs. ExPASy Swiss-Prot

BLAST of EY710539 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 11


Match Name	E-value	Identity	Description
PSMD2_DICDI	2.013e-38	58.68	26S proteasome non-ATPase regulatory subunit 2 OS=...	[more]
PSMD2_RAT	3.774e-36	49.64	26S proteasome non-ATPase regulatory subunit 2 OS=...	[more]
PSMD2_PONAB	3.774e-36	49.64	26S proteasome non-ATPase regulatory subunit 2 OS=...	[more]
PSMD2_MOUSE	3.774e-36	49.64	26S proteasome non-ATPase regulatory subunit 2 OS=...	[more]
PSMD2_HUMAN	3.774e-36	49.64	26S proteasome non-ATPase regulatory subunit 2 OS=...	[more]
PSMD2_BOVIN	3.774e-36	49.64	26S proteasome non-ATPase regulatory subunit 2 OS=...	[more]
RPN1_NEUCR	1.172e-34	55.74	26S proteasome regulatory subunit rpn-1 OS=Neurosp...	[more]
RPN1_SCHPO	4.219e-31	49.59	26S proteasome regulatory subunit rpn1 OS=Schizosa...	[more]
RPN1_YEAST	7.378e-23	51.19	26S proteasome regulatory subunit RPN1 OS=Saccharo...	[more]
RPN1_CANGA	4.655e-22	50.00	26S proteasome regulatory subunit RPN1 OS=Candida ...	[more]

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Properties


Property Name	Value
Genbank description	CS00-C3-701-024-D02-CT.F Sweet orange fruit, development stadium (2 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY710539 ID=EY710539; Name=EY710539; organism=Citrus sinensis; type=EST; length=999bp
CCGGGGAAATTTCCCAGGGTCGAACCCACGCGTCCGATTGTGATATGACA
CTGCTCTCTTGTGCATATGCTGGAACTGGGAATGTCCTCAAGGTTCAAAA
TCTTTTGGGTCATTGTGCACAGCATCATGAGAAAGGTGAAGCCTACCAAG
GACCTGCTGTGCTTGGAATTGCTATGGTAGCTATGGCTGAAGAATTGGGT
CTTGAGATGGCAATTCGATCATTAGAGCACCTTTTGCAGTATGGAGAGCA
GAACATTCGTCGTGCAGTTCCGTTGGCTCTTGGTCTCCTATGTATATCAA
ACCCAAAGGTTAATGTTATGGACACATTAAGCCGGCTAAGCCATGATACT
GATTCAGAAGTAGCTATGGCTGCCGTTATATCATTGGGTTTGATAGGGTC
GGGAACGAAACAATGCTAGAATAGCTGGCATGCTTCGCAATCTCTCAAGC
TACTACTACAAAAGATGCCAATCTTCTGTTTTGTGTCCCAATTGCTCAAG
GATTAGTGCACATGGGAAAGGGTCTCCTTGACCCCTTAACCCAAACCTTT
CTGGATCGCTTTCTTGCATCCCCGAACTGCTCTTGTGGGTAAAATAAACC
AATCTTTTTTTGCATGTCTAAACAATGAAAGGCAATAATCGTGAGGAAAA
AATCAATTATGTTGCAATCACTCCTATGACATTGGGCAAATGACGCCACA
AAATGATTATTGAATTGAATAAAAAAAAATTCACAAAACCCTCTTTCTGT
GTTCTTGTTTCGAATGGGAACCAAGAAAAATTAAAGTTTTTATACCTACG
GAAAGGTCAGGCCCCCAGAACCACATACAGTGGGTATCCACAAACACCCC
TACCCACCCCCTTATACCTTTATTGGGGTGCGATGGGAGCATACGAAAAA
AGAAACAAGGATAACAGAACAACACAAAAAATTTCTTCCTTCCTTTTCTC
ACCCCCAAATAATCTTTCACTATACATCTTTTCTATTACCCATCCTATA