EY684967

Overview
NameEY684967
Unique NameEY684967
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length890
Libraries
Library NameType
Sweet orange bark, greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of EY684967 vs. ExPASy Swiss-Prot
Match: CML15_ARATH (Probable calcium-binding protein CML15 OS=Arabidopsis thaliana GN=CML15 PE=2 SV=1)

HSP 1 Score: 173.711 bits (439), Expect = 1.240e-42
Identity = 87/153 (56.86%), Postives = 112/153 (73.20%), Query Frame = 2
Query:  134 EQLKQLKDIFMRFDMDSDGSXXXXXXXXXXXXXXXKPTGDQLHILLADMDSNGNGLVEFNELVALILPDISEQVLINQKQLMEVFRSFDRDGNGHITAAELAGSMAKMGHPLTYGELSEMMREGQPNDNGRIILVSPNTL*KKLIHNYTGLKL 592
            +Q++QLKDIF RFDMD+DGS               KP+GDQ+H+LLA MDSNGNG VEF+ELV  ILPD++E+VLIN +QL+E+F+SFDRDGNG I+AAELAG+MAKMG PLTY EL+EM++E   N +G I      ++  K   +Y GLK+
Sbjct:    3 DQIRQLKDIFDRFDMDADGSLTILELAALLRSLGLKPSGDQIHVLLASMDSNGNGFVEFDELVGTILPDLNEEVLINSEQLLEIFKSFDRDGNGFISAAELAGAMAKMGQPLTYKELTEMIKEADTNGDGVISFGEFASIMAKSAVDYFGLKI 155          
BLAST of EY684967 vs. ExPASy Swiss-Prot
Match: CML16_ARATH (Probable calcium-binding protein CML16 OS=Arabidopsis thaliana GN=CML16 PE=2 SV=1)

HSP 1 Score: 171.014 bits (432), Expect = 8.034e-42
Identity = 88/136 (64.71%), Postives = 100/136 (73.53%), Query Frame = 2
Query:  122 TVQSEQLKQLKDIFMRFDMDSDGSXXXXXXXXXXXXXXXKPTGDQLHILLADMDSNGNGLVEFNELVALILPDISEQVLINQKQLMEVFRSFDRDGNGHITAAELAGSMAKMGHPLTYGELSEMMREGQPNDNGRI 529
            T  ++Q+KQLKDIF RFDMD DGS               KP  DQ+ +LL  +D NGNG VEF+ELV  ILPDI+E+VLINQ+QLMEVFRSFDRDGNG ITAAELAGSMAKMGHPLTY EL+EMM E   N +G I
Sbjct:    4 TKPTDQIKQLKDIFARFDMDKDGSLTQLELAALLRSLGIKPRSDQISLLLNQIDRNGNGSVEFDELVVAILPDINEEVLINQEQLMEVFRSFDRDGNGSITAAELAGSMAKMGHPLTYRELTEMMTEADSNGDGVI 139          
BLAST of EY684967 vs. ExPASy Swiss-Prot
Match: CML14_ORYSJ (Probable calcium-binding protein CML14 OS=Oryza sativa subsp. japonica GN=CML14 PE=2 SV=1)

HSP 1 Score: 140.969 bits (354), Expect = 8.903e-33
Identity = 71/138 (51.45%), Postives = 97/138 (70.29%), Query Frame = 2
Query:  119 ETVQSEQLKQLKDIFMRFDMDSDGSXXXXXXXXXXXXXXXKPTGDQLHILLADMDSNGNGLVEFNELVALILPDISEQV-LINQKQLMEVFRSFDRDGNGHITAAELAGSMAKMGHPLTYGELSEMMREGQPNDNGRI 529
            + ++  QLKQL+++F RFDM+ DGS               +PTGD++H LLA MD+NGNG VEF+EL A I P ++ Q  L++Q QL+EVFR+FDRDGNG I+AAELA SMA++G PLT+ EL+ MMR+   + +G I
Sbjct:   16 QQLRGSQLKQLRELFRRFDMNGDGSLTQLELAALLRSLGLRPTGDEVHALLAGMDANGNGSVEFDELAAAIAPVLTTQTHLVDQAQLLEVFRAFDRDGNGFISAAELARSMARLGQPLTFEELTRMMRDADTDGDGVI 153          
BLAST of EY684967 vs. ExPASy Swiss-Prot
Match: CML12_ORYSJ (Probable calcium-binding protein CML12 OS=Oryza sativa subsp. japonica GN=CML12 PE=2 SV=1)

HSP 1 Score: 129.413 bits (324), Expect = 2.681e-29
Identity = 70/159 (44.03%), Postives = 99/159 (62.26%), Query Frame = 2
Query:  125 VQSEQLKQLKDIFMRFDMDSDGSXXXXXXXXXXXXXXXKPT-GDQLHILLADMDSNGNGLVEFNELVA----LILPDISEQVLINQKQLMEVFRSFDRDGNGHITAAELAGSMAKMGHPLTYGELSEMMREGQPNDNGRIILVSPNTL*KKLIHNYTGL 586
            ++ EQL+QL +IF+RFD+D DGS               +P  GD++H L+A +D++GNG VEF+EL +    LIL      V ++Q +L E FR+FDRDGNG I+AAELA SMA+MGHP+ Y EL++MMRE   + +G I       +  K   ++ GL
Sbjct:   88 LRGEQLRQLHEIFLRFDLDGDGSLTKLELAALLRSLGLRPAAGDEIHALIAAIDADGNGTVEFDELASSLADLILGPCRPSVAVDQAELAEAFRAFDRDGNGFISAAELARSMARMGHPICYAELTDMMREADTDGDGLISFEEFTAIMAKSALDFLGL 246          
BLAST of EY684967 vs. ExPASy Swiss-Prot
Match: CML18_ARATH (Probable calcium-binding protein CML18 OS=Arabidopsis thaliana GN=CML18 PE=1 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 1.166e-24
Identity = 63/132 (47.73%), Postives = 82/132 (62.12%), Query Frame = 2
Query:  134 EQLKQLKDIFMRFDMDSDGSXXXXXXXXXXXXXXXKPTGDQLHILLADMDSNGNGLVEFNELVALILPDISEQVLINQKQLMEVFRSFDRDGNGHITAAELAGSMAKMGHPLTYGELSEMMREGQPNDNGRI 529
            EQL +L++IF  FD + DGS               KP+ DQL  L+   D N NGLVEF+E VAL+ PD+ +    +  QL  +FR FDRDGNG+ITAAELA SMAK+GH LT  EL+ M++E   + +G I
Sbjct:   16 EQLAELREIFRSFDQNKDGSLTELELGSLLRSLGLKPSQDQLDTLIQKADRNNNGLVEFSEFVALVEPDLVKCPYTDD-QLKAIFRMFDRDGNGYITAAELAHSMAKLGHALTAEELTGMIKEADRDGDGCI 146          
BLAST of EY684967 vs. ExPASy Swiss-Prot
Match: CML11_ORYSJ (Probable calcium-binding protein CML11 OS=Oryza sativa subsp. japonica GN=CML11 PE=2 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 3.391e-24
Identity = 59/138 (42.75%), Postives = 86/138 (62.32%), Query Frame = 2
Query:  119 ETVQSEQLKQLKDIFMRFDMDSDGSXXXXXXXXXXXXXXXKPTGDQLHILLADMDSNGNGLVEFNELVALILPDIS-EQVLINQKQLMEVFRSFDRDGNGHITAAELAGSMAKMGHPLTYGELSEMMREGQPNDNGRI 529
            E +  +QL +L++IF  FD + DGS               KP+ D+L  L+   D+N NGL+EF+E VAL+ P++  ++   ++ Q+  +F  FDRDGNG ITAAELA SMAK+GH LT  EL+ M++E   + +GRI
Sbjct:   55 EPLGDDQLGELREIFRSFDRNGDGSLTQLELGSLLRSLGLKPSTDELDSLIQRADTNSNGLIEFSEFVALVAPELLYDRAPYSEDQIRRLFNIFDRDGNGFITAAELAHSMAKLGHALTVKELTGMIKEADTDGDGRI 192          
BLAST of EY684967 vs. ExPASy Swiss-Prot
Match: CML17_ARATH (Probable calcium-binding protein CML17 OS=Arabidopsis thaliana GN=CML17 PE=2 SV=1)

HSP 1 Score: 111.694 bits (278), Expect = 5.784e-24
Identity = 60/143 (41.96%), Postives = 83/143 (58.04%), Query Frame = 2
Query:  113 VMETVQSEQLKQLKDIFMRFDMDSDGSXXXXXXXXXXXXXXXKPTGDQLHILLADMDSNGNGLVEFNELVALILPDI----SEQVLINQKQLMEVFRSFDRDGNGHITAAELAGSMAKMGHPLTYGELSEMMREGQPNDNGRI 529
            V + +  EQ+ +L++IF  FD + DGS               KP+ DQ   L+   D+  NGLVEF E VAL+ P++           ++QL+ +FR FD DGNG ITAAELA SMAK+GH LT  EL+ M++E   + +GRI
Sbjct:    5 VSKKLDEEQINELREIFRSFDRNKDGSLTQLELGSLLRALGVKPSPDQFETLIDKADTKSNGLVEFPEFVALVSPELLSPAKRTTPYTEEQLLRLFRIFDTDGNGFITAAELAHSMAKLGHALTVAELTGMIKEADSDGDGRI 147          
BLAST of EY684967 vs. ExPASy Swiss-Prot
Match: CALM_PARTE (Calmodulin OS=Paramecium tetraurelia GN=CAM PE=1 SV=3)

HSP 1 Score: 90.5077 bits (223), Expect = 1.380e-17
Identity = 48/137 (35.04%), Postives = 78/137 (56.93%), Query Frame = 2
Query:  119 ETVQSEQLKQLKDIFMRFDMDSDGSXXXXXXXXXXXXXXXKPTGDQLHILLADMDSNGNGLVEFNELVALILPDISEQVLINQKQLMEVFRSFDRDGNGHITAAELAGSMAKMGHPLTYGELSEMMREGQPNDNGRI 529
            E +  EQ+ + K+ F  FD D DG+                PT  +L  ++ ++D++GNG ++F E ++L+   + EQ   ++++L+E F+ FDRDGNG I+AAEL   M  +G  LT  E+ EM+RE   + +G I
Sbjct:    3 EQLTEEQIAEFKEAFALFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLSLMARKMKEQD--SEEELIEAFKVFDRDGNGLISAAELRHVMTNLGEKLTDDEVDEMIREADIDGDGHI 137          
BLAST of EY684967 vs. ExPASy Swiss-Prot
Match: CALM_HETTR (Calmodulin OS=Heterocapsa triquetra PE=2 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 8.945e-17
Identity = 46/137 (33.58%), Postives = 77/137 (56.20%), Query Frame = 2
Query:  119 ETVQSEQLKQLKDIFMRFDMDSDGSXXXXXXXXXXXXXXXKPTGDQLHILLADMDSNGNGLVEFNELVALILPDISEQVLINQKQLMEVFRSFDRDGNGHITAAELAGSMAKMGHPLTYGELSEMMREGQPNDNGRI 529
            + +  EQ+ + K+ F  FD D DG+                PT  +L  ++ ++DS+GNG ++F E ++L+   + +     +++L+E F+ FDRDGNG I+AAEL   M  +G  LT  E+ EM+RE   + +G+I
Sbjct:    3 DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDSDGNGTIDFPEFLSLMARKMKDTD--TEEELIEAFKVFDRDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADVDGDGQI 137          
BLAST of EY684967 vs. ExPASy Swiss-Prot
Match: CALM_DICDI (Calmodulin OS=Dictyostelium discoideum GN=calA PE=1 SV=3)

HSP 1 Score: 87.0409 bits (214), Expect = 1.526e-16
Identity = 44/141 (31.21%), Postives = 78/141 (55.32%), Query Frame = 2
Query:  107 MTVMETVQSEQLKQLKDIFMRFDMDSDGSXXXXXXXXXXXXXXXKPTGDQLHILLADMDSNGNGLVEFNELVALILPDISEQVLINQKQLMEVFRSFDRDGNGHITAAELAGSMAKMGHPLTYGELSEMMREGQPNDNGRI 529
            M   E++  EQ+ + K+ F  FD D DGS                PT  +L  ++ ++D++GNG ++F E + ++   + +     ++++ E F+ FD+DGNG+I+AAEL   M  +G  LT  E+ EM+RE   + +G++
Sbjct:    1 MASQESLTEEQIAEFKEAFSLFDKDGDGSITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGNIDFPEFLTMMARKMQDTD--TEEEIREAFKVFDKDGNGYISAAELRHVMTSLGEKLTNEEVDEMIREADLDGDGQV 139          
The following BLAST results are available for this feature:
BLAST of EY684967 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 154
Match NameE-valueIdentityDescription
CML15_ARATH1.240e-4256.86Probable calcium-binding protein CML15 OS=Arabidop... [more]
CML16_ARATH8.034e-4264.71Probable calcium-binding protein CML16 OS=Arabidop... [more]
CML14_ORYSJ8.903e-3351.45Probable calcium-binding protein CML14 OS=Oryza sa... [more]
CML12_ORYSJ2.681e-2944.03Probable calcium-binding protein CML12 OS=Oryza sa... [more]
CML18_ARATH1.166e-2447.73Probable calcium-binding protein CML18 OS=Arabidop... [more]
CML11_ORYSJ3.391e-2442.75Probable calcium-binding protein CML11 OS=Oryza sa... [more]
CML17_ARATH5.784e-2441.96Probable calcium-binding protein CML17 OS=Arabidop... [more]
CALM_PARTE1.380e-1735.04Calmodulin OS=Paramecium tetraurelia GN=CAM PE=1 S... [more]
CALM_HETTR8.945e-1733.58Calmodulin OS=Heterocapsa triquetra PE=2 SV=1[more]
CALM_DICDI1.526e-1631.21Calmodulin OS=Dictyostelium discoideum GN=calA PE=... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C2-003-007-A03-CT.F Sweet orange bark, greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY684967 ID=EY684967; Name=EY684967; organism=Citrus sinensis; type=EST; length=890bp
AAAAAAAAGAACACAAAACACAACCTTTTTTTTTTTTTTCTTTTTCTTTT
TTTTTTCTTGAATCTTATTTTTCTTTCACCCCATTCCCATATATCTTTAA
TCAACAATGACGGTGATGGAAACAGTTCAATCGGAGCAACTGAAGCAGCT
CAAAGACATATTCATGCGTTTCGACATGGACTCGGACGGCAGCCTCACGC
AGCTGGAACTGGCCGCACTCCTGCGTGCCCTGGGGCTCAAGCCAACCGGC
GACCAGCTGCACATTTTGTTGGCCGACATGGATTCCAACGGCAACGGCCT
CGTCGAATTCAACGAATTGGTTGCTCTGATACTCCCCGACATCAGCGAGC
AAGTCTTGATCAACCAAAAACAACTCATGGAGGTCTTTCGGTCCTTCGAT
CGCGACGGCAACGGCCACATCACTGCGGCCGAGCTCGCGGGCTCCATGGC
CAAAATGGGCCACCCTTTGACGTACGGCGAGCTATCGGAAATGATGAGGG
AGGGCCAACCCAATGACAACGGACGCATAATCTTAGTGAGTCCGAACACA
TTATGAAAAAAGCTGATCCACAACTATACCGGCCTTAAGCTTAGGGGCGG
CCACCCGAGCACCCAAACAAAAAAAAAAAACAAACGCCCATGCGACGCAG
CTGCATGGGACACAGGACCGACCCCACTCAACCCGCGAGAACACCCAAAA
GAACCCGAGAGCGGACAAGAGCGCGCCAAGATTCAAGAAGGAGACAAGAA
CGTGACAAAAGCAGAGAAAGAGAGACAGCGGGCCGGGCGGGAACGGAGTA
CGCGAGAAAAGCCCAAACGGTCAAAAATAACACACGACACAGTAAAAAAG
ACACAACACGAAACGAACACAACGACCAAAGACGGCAGAG
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