EY710300

Overview
NameEY710300
Unique NameEY710300
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length1165
Libraries
Library NameType
Sweet orange fruit, development stadium (2 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY710300 vs. ExPASy Swiss-Prot
Match: EF1A_TOBAC (Elongation factor 1-alpha OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 177.178 bits (448), Expect = 3.763e-46
Identity = 88/109 (80.73%), Postives = 96/109 (88.07%), Query Frame = 3 
Query:   60 FLLDKLKAERERGITIDIALWKFETSKYYCTVIDAPGHRDFNKNMITDTSQADCAVLIIDSTTGGFQAGISQDGLSPEHALHAFSLGVNQMISSSNKMDATTSIYLKLK 386
            ++LDKLKAERERGITIDIALWKFET+KYYCTVIDAPGHRDF KNMIT TSQADCAVLIIDSTTGGF+AGIS+DG + EHAL AF+LGV QMI   NKMDATT  Y K +
Sbjct:   58 WVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKAR 166

HSP 2 Score: 31.187 bits (69), Expect = 3.763e-46
Identity = 17/29 (58.62%), Postives = 19/29 (65.52%), Query Frame = 2 
Query:  374 SKAKDNEYEKEVSSKLNKEGYIPK*IPIV 460
            SKA+ +E  KEVSS L K GY P  IP V
Sbjct:  163 SKARYDEIVKEVSSYLKKVGYNPDKIPFV 191
[Close]
BLAST of EY710300 vs. ExPASy Swiss-Prot
Match: EF1A_SOLLC (Elongation factor 1-alpha OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 177.178 bits (448), Expect = 3.763e-46
Identity = 88/109 (80.73%), Postives = 96/109 (88.07%), Query Frame = 3 
Query:   60 FLLDKLKAERERGITIDIALWKFETSKYYCTVIDAPGHRDFNKNMITDTSQADCAVLIIDSTTGGFQAGISQDGLSPEHALHAFSLGVNQMISSSNKMDATTSIYLKLK 386
            ++LDKLKAERERGITIDIALWKFET+KYYCTVIDAPGHRDF KNMIT TSQADCAVLIIDSTTGGF+AGIS+DG + EHAL AF+LGV QMI   NKMDATT  Y K +
Sbjct:   58 WVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKAR 166

HSP 2 Score: 31.187 bits (69), Expect = 3.763e-46
Identity = 17/29 (58.62%), Postives = 19/29 (65.52%), Query Frame = 2 
Query:  374 SKAKDNEYEKEVSSKLNKEGYIPK*IPIV 460
            SKA+ +E  KEVSS L K GY P  IP V
Sbjct:  163 SKARYDEIVKEVSSYLKKVGYNPDKIPFV 191
[Close]
BLAST of EY710300 vs. ExPASy Swiss-Prot
Match: EF1A_ORYSJ (Elongation factor 1-alpha OS=Oryza sativa subsp. japonica GN=REFA1 PE=2 SV=2)

HSP 1 Score: 177.178 bits (448), Expect = 3.763e-46
Identity = 88/109 (80.73%), Postives = 96/109 (88.07%), Query Frame = 3 
Query:   60 FLLDKLKAERERGITIDIALWKFETSKYYCTVIDAPGHRDFNKNMITDTSQADCAVLIIDSTTGGFQAGISQDGLSPEHALHAFSLGVNQMISSSNKMDATTSIYLKLK 386
            ++LDKLKAERERGITIDIALWKFET+KYYCTVIDAPGHRDF KNMIT TSQADCAVLIIDSTTGGF+AGIS+DG + EHAL AF+LGV QMI   NKMDATT  Y K +
Sbjct:   58 WVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKAR 166

HSP 2 Score: 31.187 bits (69), Expect = 3.763e-46
Identity = 17/29 (58.62%), Postives = 19/29 (65.52%), Query Frame = 2 
Query:  374 SKAKDNEYEKEVSSKLNKEGYIPK*IPIV 460
            SKA+ +E  KEVSS L K GY P  IP V
Sbjct:  163 SKARYDEIVKEVSSYLKKVGYNPDKIPFV 191
[Close]
BLAST of EY710300 vs. ExPASy Swiss-Prot
Match: EF1A_MANES (Elongation factor 1-alpha OS=Manihot esculenta GN=EF1 PE=3 SV=1)

HSP 1 Score: 177.178 bits (448), Expect = 3.763e-46
Identity = 88/109 (80.73%), Postives = 96/109 (88.07%), Query Frame = 3 
Query:   60 FLLDKLKAERERGITIDIALWKFETSKYYCTVIDAPGHRDFNKNMITDTSQADCAVLIIDSTTGGFQAGISQDGLSPEHALHAFSLGVNQMISSSNKMDATTSIYLKLK 386
            ++LDKLKAERERGITIDIALWKFET+KYYCTVIDAPGHRDF KNMIT TSQADCAVLIIDSTTGGF+AGIS+DG + EHAL AF+LGV QMI   NKMDATT  Y K +
Sbjct:   58 WVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKAR 166

HSP 2 Score: 31.187 bits (69), Expect = 3.763e-46
Identity = 17/29 (58.62%), Postives = 19/29 (65.52%), Query Frame = 2 
Query:  374 SKAKDNEYEKEVSSKLNKEGYIPK*IPIV 460
            SKA+ +E  KEVSS L K GY P  IP V
Sbjct:  163 SKARYDEIVKEVSSYLKKVGYNPDKIPFV 191
[Close]
BLAST of EY710300 vs. ExPASy Swiss-Prot
Match: EF1A_ARATH (Elongation factor 1-alpha OS=Arabidopsis thaliana GN=A1 PE=1 SV=1)

HSP 1 Score: 177.178 bits (448), Expect = 4.905e-46
Identity = 88/109 (80.73%), Postives = 96/109 (88.07%), Query Frame = 3 
Query:   60 FLLDKLKAERERGITIDIALWKFETSKYYCTVIDAPGHRDFNKNMITDTSQADCAVLIIDSTTGGFQAGISQDGLSPEHALHAFSLGVNQMISSSNKMDATTSIYLKLK 386
            ++LDKLKAERERGITIDIALWKFET+KYYCTVIDAPGHRDF KNMIT TSQADCAVLIIDSTTGGF+AGIS+DG + EHAL AF+LGV QMI   NKMDATT  Y K +
Sbjct:   58 WVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKAR 166

HSP 2 Score: 30.8018 bits (68), Expect = 4.905e-46
Identity = 17/29 (58.62%), Postives = 19/29 (65.52%), Query Frame = 2 
Query:  374 SKAKDNEYEKEVSSKLNKEGYIPK*IPIV 460
            SKA+ +E  KEVSS L K GY P  IP V
Sbjct:  163 SKARYDEIIKEVSSYLKKVGYNPDKIPFV 191
[Close]
BLAST of EY710300 vs. ExPASy Swiss-Prot
Match: EF1A_WHEAT (Elongation factor 1-alpha OS=Triticum aestivum GN=TEF1 PE=2 SV=1)

HSP 1 Score: 177.178 bits (448), Expect = 6.392e-46
Identity = 88/109 (80.73%), Postives = 96/109 (88.07%), Query Frame = 3 
Query:   60 FLLDKLKAERERGITIDIALWKFETSKYYCTVIDAPGHRDFNKNMITDTSQADCAVLIIDSTTGGFQAGISQDGLSPEHALHAFSLGVNQMISSSNKMDATTSIYLKLK 386
            ++LDKLKAERERGITIDIALWKFET+KYYCTVIDAPGHRDF KNMIT TSQADCAVLIIDSTTGGF+AGIS+DG + EHAL AF+LGV QMI   NKMDATT  Y K +
Sbjct:   58 WVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKAR 166

HSP 2 Score: 30.4166 bits (67), Expect = 6.392e-46
Identity = 16/29 (55.17%), Postives = 18/29 (62.07%), Query Frame = 2 
Query:  374 SKAKDNEYEKEVSSKLNKEGYIPK*IPIV 460
            SKA+  E  KEVSS L K GY P  +P V
Sbjct:  163 SKARYEEIVKEVSSYLKKVGYNPDKVPFV 191
[Close]
BLAST of EY710300 vs. ExPASy Swiss-Prot
Match: EF1A_SOYBN (Elongation factor 1-alpha OS=Glycine max GN=TEFS1 PE=3 SV=2)

HSP 1 Score: 176.022 bits (445), Expect = 8.330e-46
Identity = 87/109 (79.82%), Postives = 96/109 (88.07%), Query Frame = 3 
Query:   60 FLLDKLKAERERGITIDIALWKFETSKYYCTVIDAPGHRDFNKNMITDTSQADCAVLIIDSTTGGFQAGISQDGLSPEHALHAFSLGVNQMISSSNKMDATTSIYLKLK 386
            ++LDKLKAERERGITIDIALWKFET+KYYCTVIDAPGHRDF KNMIT TSQADCAVLIIDSTTGGF+AGIS+DG + EHAL +F+LGV QMI   NKMDATT  Y K +
Sbjct:   58 WVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLSFTLGVKQMICCCNKMDATTPKYSKAR 166

HSP 2 Score: 31.187 bits (69), Expect = 8.330e-46
Identity = 17/29 (58.62%), Postives = 19/29 (65.52%), Query Frame = 2 
Query:  374 SKAKDNEYEKEVSSKLNKEGYIPK*IPIV 460
            SKA+ +E  KEVSS L K GY P  IP V
Sbjct:  163 SKARYDEIVKEVSSYLKKVGYNPDKIPFV 191
[Close]
BLAST of EY710300 vs. ExPASy Swiss-Prot
Match: EF1A_MAIZE (Elongation factor 1-alpha OS=Zea mays GN=EF1A PE=3 SV=1)

HSP 1 Score: 177.178 bits (448), Expect = 4.081e-45
Identity = 88/109 (80.73%), Postives = 96/109 (88.07%), Query Frame = 3 
Query:   60 FLLDKLKAERERGITIDIALWKFETSKYYCTVIDAPGHRDFNKNMITDTSQADCAVLIIDSTTGGFQAGISQDGLSPEHALHAFSLGVNQMISSSNKMDATTSIYLKLK 386
            ++LDKLKAERERGITIDIALWKFET+KYYCTVIDAPGHRDF KNMIT TSQADCAVLIIDSTTGGF+AGIS+DG + EHAL AF+LGV QMI   NKMDATT  Y K +
Sbjct:   58 WVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKAR 166

HSP 2 Score: 27.7202 bits (60), Expect = 4.081e-45
Identity = 16/29 (55.17%), Postives = 18/29 (62.07%), Query Frame = 2 
Query:  374 SKAKDNEYEKEVSSKLNKEGYIPK*IPIV 460
            SKA+ +E  KEVSS L K GY P  I  V
Sbjct:  163 SKARYDEIVKEVSSYLKKVGYNPDKIHFV 191
[Close]
BLAST of EY710300 vs. ExPASy Swiss-Prot
Match: EF1A1_DAUCA (Elongation factor 1-alpha OS=Daucus carota PE=3 SV=1)

HSP 1 Score: 175.252 bits (443), Expect = 6.931e-45
Identity = 87/109 (79.82%), Postives = 95/109 (87.16%), Query Frame = 3 
Query:   60 FLLDKLKAERERGITIDIALWKFETSKYYCTVIDAPGHRDFNKNMITDTSQADCAVLIIDSTTGGFQAGISQDGLSPEHALHAFSLGVNQMISSSNKMDATTSIYLKLK 386
            ++LDKLKAERERGITIDIALWKFET+KYYCTVIDAPGHRDF KNMIT TSQADCAVLIID TTGGF+AGIS+DG + EHAL AF+LGV QMI   NKMDATT  Y K +
Sbjct:   58 WVLDKLKAERERGITIDIALWKFETNKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDPTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKAR 166

HSP 2 Score: 28.8758 bits (63), Expect = 6.931e-45
Identity = 16/29 (55.17%), Postives = 19/29 (65.52%), Query Frame = 2 
Query:  374 SKAKDNEYEKEVSSKLNKEGYIPK*IPIV 460
            SKA+ +E  KEVSS L K GY P+ I  V
Sbjct:  163 SKARYDEIVKEVSSYLKKVGYNPEKIAFV 191
[Close]
BLAST of EY710300 vs. ExPASy Swiss-Prot
Match: EF1A_VICFA (Elongation factor 1-alpha OS=Vicia faba PE=2 SV=1)

HSP 1 Score: 177.948 bits (450), Expect = 9.661e-44
Identity = 89/107 (83.18%), Postives = 95/107 (88.79%), Query Frame = 3 
Query:   60 FLLDKLKAERERGITIDIALWKFETSKYYCTVIDAPGHRDFNKNMITDTSQADCAVLIIDSTTGGFQAGISQDGLSPEHALHAFSLGVNQMISSSNKMDATTSIYLK 380
            ++LDKLKAERERGITIDIALWKFETSKYYCTVIDAPGHRDF KNMIT TSQADCAVLIIDSTTGGF+AGIS+DG + EHAL AF+LGV QMI   NKMDATT  Y K
Sbjct:   58 WVLDKLKAERERGITIDIALWKFETSKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSK 164
The following BLAST results are available for this feature:
BLAST of EY710300 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 386
Match NameE-valueIdentityDescription
EF1A_TOBAC3.763e-4680.73Elongation factor 1-alpha OS=Nicotiana tabacum PE=... [more]
EF1A_SOLLC3.763e-4680.73Elongation factor 1-alpha OS=Solanum lycopersicum ... [more]
EF1A_ORYSJ3.763e-4680.73Elongation factor 1-alpha OS=Oryza sativa subsp. j... [more]
EF1A_MANES3.763e-4680.73Elongation factor 1-alpha OS=Manihot esculenta GN=... [more]
EF1A_ARATH4.905e-4680.73Elongation factor 1-alpha OS=Arabidopsis thaliana ... [more]
EF1A_WHEAT6.392e-4680.73Elongation factor 1-alpha OS=Triticum aestivum GN=... [more]
EF1A_SOYBN8.330e-4679.82Elongation factor 1-alpha OS=Glycine max GN=TEFS1 ... [more]
EF1A_MAIZE4.081e-4580.73Elongation factor 1-alpha OS=Zea mays GN=EF1A PE=3... [more]
EF1A1_DAUCA6.931e-4579.82Elongation factor 1-alpha OS=Daucus carota PE=3 SV... [more]
EF1A_VICFA9.661e-4483.18Elongation factor 1-alpha OS=Vicia faba PE=2 SV=1[more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-701-097-C04-CT.F Sweet orange fruit, development stadium (2 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY710300 ID=EY710300; Name=EY710300; organism=Citrus sinensis; type=EST; length=1165bp
GGGTTTCCGGAACCCCCCGCCGTTCCCTTATTAGAACATGAGGTCATTCA
AATACCCCTTTTTTGCTCGACAAGCTCAAGGCTGAGCGTGAACGTGGTAT
TACCATTGATATTGCCTTGTGGAAGTTCGAGACCTCCAAGTACTACTGCA
CCGTCATTGATGCTCCTGGACATCGTGACTTTAACAAGAACATGATTACT
GATACCTCACAAGCTGACTGTGCCGTCCTTATCATTGACTCTACCACCGG
TGGTTTTCAAGCTGGTATCTCCCAGGATGGTCTATCTCCTGAGCATGCTT
TGCATGCATTCTCCCTTGGTGTGAACCAAATGATTAGCAGCAGTAACAAG
ATGGATGCAACCACCTCCATATATCTAAAGCTAAAGACAATGAATATGAG
AAAGAGGTCTCTTCCAAATTGAATAAAGAAGGATACATTCCCAAATAAAT
CCCAATCGTAGTCCTCCTCTGGTCTCAAGAGTGTCTTCAGTATTGAAGAT
CTTTCTACCATGTCTTGAAACTTGGACTATCATAGCAGGATAATCTTTGT
AAAATTAGGGATAAGAAAAGACATAATACATGTACAATATATAGCACAGT
AAAACAGGAACAAAATTGTTGGAATTGAAACCTTCCATAGGTCATGCATT
TAACAGATGGACCAAACAAGTTAGATGATAAAATTTTGCTCCCTCTATCT
GAACTTCGTATATTAATATTGTGATTTACTCTTCTTCTTTTATAGAATAG
TTCCTGGAACAATAGTTTTATTAAAACATAGAACTTTATTTCAAGATACG
AGCTGTGTTTTGTGTGATATACATGAAAGGAAAACTAATATGAATTTTTT
ATATTAATATTGGTTTAATAGAATATAATGTGTCAGAGGATTGTGTATTA
TATTTATATAAAATGTCTAATAATATATTAGAAACACATACAGGTAATAT
GTAAAACATACTATATTTAAAGTATATGAAACATGATATATGTACAAAAG
ATAAACTGCAATTCATATACTATTACTCTCCCCAATTTTTATTGCTTGAT
AATATTTCCATGAATATTACATTAATGTGTATCTTACACACTGTCTAATT
AAATGTTATTTTTATTATATTTCTATTATTCATTTATATATACACTAATT
TGTACATGATGAGTA
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