EY684494 | Citrus Genome Database

Overview


Name	EY684494
Unique Name	EY684494
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	921

Libraries


Library Name	Type
Sweet orange leaf, young greenhouse plant	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY684494 vs. ExPASy Swiss-Prot
Match: 4CL2_SOYBN (4-coumarate--CoA ligase 2 OS=Glycine max PE=2 SV=2)

HSP 1 Score: 131.724 bits (330), Expect = 7.270e-37
Identity = 68/116 (58.62%), Postives = 83/116 (71.55%), Query Frame = 3

Query:   78 MISIATKKPELSLDISSAAPPAPSNEKIATHIFKSKLPDIPISNHLPLHTYCFQD--RLSDDPCLIVGSTGKTYSYAETHLICRKTAAGLSNLGIKKGDVIMILLQNCAEFVFSFM 419
            MI++A        D +  + P  S      H+FKSKLPDIPISNHLPLH+YCFQ+  + +  PCLIVG   KT++YA+THLI  K AAGLSNLGI KGDV+MILLQN A+FVFSF+
Sbjct:    1 MITLAPSLDTPKTDQNQVSDPQTS------HVFKSKLPDIPISNHLPLHSYCFQNLSQFAHRPCLIVGPASKTFTYADTHLISSKIAAGLSNLGILKGDVVMILLQNSADFVFSFL 110

HSP 2 Score: 44.2838 bits (103), Expect = 7.270e-37
Identity = 39/111 (35.14%), Postives = 54/111 (48.65%), Query Frame = 2

Query:  425 LSMIGAVTTTANPFYTSAEILKQFKNLWGPN*LIT----LVPVSQRGCQKTEQEFPQWIPIDAPPEKFAAFPRVIREAK*GSIPK-NWHPGPERNRGGCPSFRGQRDWPKG 742
            +SMIGAV TTANPFYT+ EI KQF  +     +IT    +  +      K  ++F + + +D PPE    F  V+ EA    +P+   HP    +    P   G    PKG
Sbjct:  112 ISMIGAVATTANPFYTAPEIFKQF-TVSKAKLIITQAMYVDKLRNHDGAKLGEDF-KVVTVDDPPENCLHF-SVLSEANESDVPEVEIHPD---DAVAMPFSSGTTGLPKG 216

[Close]

BLAST of EY684494 vs. ExPASy Swiss-Prot
Match: 4CL3_ARATH (4-coumarate--CoA ligase 3 OS=Arabidopsis thaliana GN=4CL3 PE=1 SV=1)

HSP 1 Score: 142.895 bits (359), Expect = 2.450e-33
Identity = 83/163 (50.92%), Postives = 106/163 (65.03%), Query Frame = 3

Query:   78 MISIATKKPEL---------SLDISSAAPPAPSNEKIATHIFKSKLPDIPISNHLPLHTYCFQ--DRLSDDPCLIVGSTGKTYSYAETHLICRKTAAGLSNLGIKKGDVIMILLQNCAEFVFSFMG-SFP*SVQSRRRPIPSTLQLKS*NSSKTSGGQID*SH 530
            MI+ A  +P++         S D+   +PP P        IF+SKLPDI I NHLPLHTYCF+    +SD PCLIVGSTGK+Y+Y ETHLICR+ A+GL  LGI+KGDVIMILLQN AEFVFSFMG S   +V +   P  ++ +L      K+SG ++  +H
Sbjct:    1 MITAALHEPQIHKPTDTSVVSDDVLPHSPPTP-------RIFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLYKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELY--KQLKSSGAKLIITH 154

[Close]

BLAST of EY684494 vs. ExPASy Swiss-Prot
Match: 4CL2_ORYSJ (Probable 4-coumarate--CoA ligase 2 OS=Oryza sativa subsp. japonica GN=4CL2 PE=2 SV=2)

HSP 1 Score: 112.849 bits (281), Expect = 1.014e-28
Identity = 57/118 (48.31%), Postives = 76/118 (64.41%), Query Frame = 3

Query:   78 MISIATKKPELSLDISSAAPPAPSNEKIATHIFKSKLPDIPISNHLPLHTYCFQD--RLSDDPCLIVGSTGKTYSYAETHLICRKTAAGLSNLGIKKGDVIMILLQNCAEFVFSFMGS 425
            MI++A   PE    +++A   AP     A  +F+SKLPDI I +HLPLH YCF     L D PCLI  +TG+TY++AET L+CR+ AA L  LG+  GD +M+LLQNC EF  +F  +
Sbjct:    1 MITVAA--PEAQPQVAAAVDEAPPE---AVTVFRSKLPDIDIPSHLPLHEYCFARAAELPDAPCLIAAATGRTYTFAETRLLCRRAAAALHRLGVGHGDRVMVLLQNCVEFAVAFFAA 113

HSP 2 Score: 35.8094 bits (81), Expect = 1.014e-28
Identity = 17/24 (70.83%), Postives = 18/24 (75.00%), Query Frame = 2

Query:  428 SMIGAVTTTANPFYTSAEILKQFK 499
            S +GAVTT ANPF T  EI KQFK
Sbjct:  114 SFLGAVTTAANPFCTPQEIHKQFK 137

[Close]

BLAST of EY684494 vs. ExPASy Swiss-Prot
Match: 4CL2_ARATH (4-coumarate--CoA ligase 2 OS=Arabidopsis thaliana GN=4CL2 PE=1 SV=2)

HSP 1 Score: 97.4413 bits (241), Expect = 4.164e-28
Identity = 47/87 (54.02%), Postives = 62/87 (71.26%), Query Frame = 3

Query:  171 IFKSKLPDIPISNHLPLHTYCFQD--RLSDDPCLIVGSTGKTYSYAETHLICRKTAAGLSNLGIKKGDVIMILLQNCAEFVFSFMGS 425
            IF+S+LPDI I NHLPLH Y F++    +  PCLI G TG+ Y+YA+ H+  RK AAGL NLG+K+ DV+MILL N  E V +F+ +
Sbjct:   22 IFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLAAGLHNLGVKQHDVVMILLPNSPEVVLTFLAA 108

HSP 2 Score: 37.7354 bits (86), Expect = 4.164e-28
Identity = 17/24 (70.83%), Postives = 20/24 (83.33%), Query Frame = 2

Query:  428 SMIGAVTTTANPFYTSAEILKQFK 499
            S IGA+TT+ANPF+T AEI KQ K
Sbjct:  109 SFIGAITTSANPFFTPAEISKQAK 132

HSP 3 Score: 31.5722 bits (70), Expect = 4.164e-28
Identity = 24/66 (36.36%), Postives = 33/66 (50.00%), Query Frame = 1

Query:  541 KSKGLPKDGTRISTMDSDRCPPGKICRISQGYPGSQMRIN--PQKLASRPGKKPGGLPFFSGTKGL 732
            K K L  DG  I T DSD  P   + R S+     + R++  P+K++    +    LPF SGT GL
Sbjct:  148 KIKNLQNDGVLIVTTDSDAIPENCL-RFSELTQSEEPRVDSIPEKISP---EDVVALPFSSGTTGL 209

[Close]

BLAST of EY684494 vs. ExPASy Swiss-Prot
Match: 4CL_PINTA (4-coumarate--CoA ligase OS=Pinus taeda GN=4CL PE=2 SV=1)

HSP 1 Score: 111.694 bits (278), Expect = 1.090e-27
Identity = 51/92 (55.43%), Postives = 69/92 (75.00%), Query Frame = 3

Query:  156 KIATHIFKSKLPDIPISNHLPLHTYCFQD--RLSDDPCLIVGSTGKTYSYAETHLICRKTAAGLSNLGIKKGDVIMILLQNCAEFVFSFMGS 425
            K   H+++SKLPDI IS+HLPLH+YCF+     +D PCLI G+T +TY ++E  LI RK AAGL+ LG+++G V+M+LL NC EF F FMG+
Sbjct:    6 KKVEHLYRSKLPDIEISDHLPLHSYCFERVAEFADRPCLIDGATDRTYCFSEVELISRKVAAGLAKLGLQQGQVVMLLLPNCIEFAFVFMGA 97

HSP 2 Score: 33.4982 bits (75), Expect = 1.090e-27
Identity = 25/73 (34.25%), Postives = 35/73 (47.95%), Query Frame = 2

Query:  428 SMIGAVTTTANPFYTSAEILKQFKNLWGPN*LITLVPVSQRGCQKTEQEFPQWIPIDAPPEKFAAFPRVIREA 646
            S+ GA+ TTANPFY   EI KQ K   G   ++TL    ++       +    I ID  P++      V+ EA
Sbjct:   98 SVRGAIVTTANPFYKPGEIAKQAKAA-GARIIVTLAAYVEKLADLQSHDV-LVITIDDAPKEGCQHISVLTEA 168

[Close]

BLAST of EY684494 vs. ExPASy Swiss-Prot
Match: 4CL2_PETCR (4-coumarate--CoA ligase 1 OS=Petroselinum crispum GN=4CL2 PE=2 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 2.580e-26
Identity = 47/87 (54.02%), Postives = 68/87 (78.16%), Query Frame = 3

Query:  171 IFKSKLPDIPISNHLPLHTYCFQD--RLSDDPCLIVGSTGKTYSYAETHLICRKTAAGLSNLGIKKGDVIMILLQNCAEFVFSFMGS 425
            IF+SKLPDI I  HLPLHTYCF++  ++ D  CLI G+TG+T++Y++  L+ RK A+GL+ LGI++GD IM+LL N  E+ F+F+G+
Sbjct:   12 IFRSKLPDIYIPKHLPLHTYCFENISKVGDKSCLINGATGETFTYSQVELLSRKVASGLNKLGIQQGDTIMLLLPNSPEYFFAFLGA 98

HSP 2 Score: 36.1946 bits (82), Expect = 2.580e-26
Identity = 14/21 (66.67%), Postives = 19/21 (90.48%), Query Frame = 2

Query:  437 GAVTTTANPFYTSAEILKQFK 499
            GA++T ANPF+TSAE++KQ K
Sbjct:  102 GAISTMANPFFTSAEVIKQLK 122

[Close]

BLAST of EY684494 vs. ExPASy Swiss-Prot
Match: 4CL1_PETCR (4-coumarate--CoA ligase 1 OS=Petroselinum crispum GN=4CL1 PE=2 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 2.580e-26
Identity = 47/87 (54.02%), Postives = 68/87 (78.16%), Query Frame = 3

Query:  171 IFKSKLPDIPISNHLPLHTYCFQD--RLSDDPCLIVGSTGKTYSYAETHLICRKTAAGLSNLGIKKGDVIMILLQNCAEFVFSFMGS 425
            IF+SKLPDI I  HLPLHTYCF++  ++ D  CLI G+TG+T++Y++  L+ RK A+GL+ LGI++GD IM+LL N  E+ F+F+G+
Sbjct:   12 IFRSKLPDIYIPKHLPLHTYCFENISKVGDKSCLINGATGETFTYSQVELLSRKVASGLNKLGIQQGDTIMLLLPNSPEYFFAFLGA 98

HSP 2 Score: 36.1946 bits (82), Expect = 2.580e-26
Identity = 14/21 (66.67%), Postives = 19/21 (90.48%), Query Frame = 2

Query:  437 GAVTTTANPFYTSAEILKQFK 499
            GA++T ANPF+TSAE++KQ K
Sbjct:  102 GAISTMANPFFTSAEVIKQLK 122

[Close]

BLAST of EY684494 vs. ExPASy Swiss-Prot
Match: 4CL1_TOBAC (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum GN=4CL1 PE=2 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 2.125e-25
Identity = 51/87 (58.62%), Postives = 63/87 (72.41%), Query Frame = 3

Query:  171 IFKSKLPDIPISNHLPLHTYCFQD--RLSDDPCLIVGSTGKTYSYAETHLICRKTAAGLSNLGIKKGDVIMILLQNCAEFVFSFMGS 425
            IF+SKLPDI I  HLPLH+YCF++    S  PCLI G+  + Y+YAE  L CRK A GL+ LGI++ D IMILL N  EFVF+FMG+
Sbjct:   16 IFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANDQIYTYAEVELTCRKVAVGLNKLGIQQKDTIMILLPNSPEFVFAFMGA 102

HSP 2 Score: 32.7278 bits (73), Expect = 2.125e-25
Identity = 13/24 (54.17%), Postives = 19/24 (79.17%), Query Frame = 2

Query:  428 SMIGAVTTTANPFYTSAEILKQFK 499
            S +GA++T ANP +T AE++KQ K
Sbjct:  103 SYLGAISTMANPLFTPAEVVKQAK 126

[Close]

BLAST of EY684494 vs. ExPASy Swiss-Prot
Match: 4CL2_SOLTU (4-coumarate--CoA ligase 2 OS=Solanum tuberosum GN=4CL2 PE=3 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 1.033e-24
Identity = 49/87 (56.32%), Postives = 63/87 (72.41%), Query Frame = 3

Query:  171 IFKSKLPDIPISNHLPLHTYCFQD--RLSDDPCLIVGSTGKTYSYAETHLICRKTAAGLSNLGIKKGDVIMILLQNCAEFVFSFMGS 425
            IF+SKLPDI I  HLPLH+YCF++    +  PCLI G+  + Y+YAE  L  RK A GL+ LGI++ D IMILL NC EFVF+F+G+
Sbjct:   14 IFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVELTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGA 100

HSP 2 Score: 32.7278 bits (73), Expect = 1.033e-24
Identity = 13/24 (54.17%), Postives = 19/24 (79.17%), Query Frame = 2

Query:  428 SMIGAVTTTANPFYTSAEILKQFK 499
            S +GA++T ANP +T AE++KQ K
Sbjct:  101 SYLGAISTMANPLFTPAEVVKQAK 124

[Close]

BLAST of EY684494 vs. ExPASy Swiss-Prot
Match: 4CL1_SOLTU (4-coumarate--CoA ligase 1 OS=Solanum tuberosum GN=4CL1 PE=3 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 1.033e-24
Identity = 49/87 (56.32%), Postives = 63/87 (72.41%), Query Frame = 3

Query:  171 IFKSKLPDIPISNHLPLHTYCFQD--RLSDDPCLIVGSTGKTYSYAETHLICRKTAAGLSNLGIKKGDVIMILLQNCAEFVFSFMGS 425
            IF+SKLPDI I  HLPLH+YCF++    +  PCLI G+  + Y+YAE  L  RK A GL+ LGI++ D IMILL NC EFVF+F+G+
Sbjct:   14 IFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVELTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGA 100

HSP 2 Score: 32.7278 bits (73), Expect = 1.033e-24
Identity = 13/24 (54.17%), Postives = 19/24 (79.17%), Query Frame = 2

Query:  428 SMIGAVTTTANPFYTSAEILKQFK 499
            S +GA++T ANP +T AE++KQ K
Sbjct:  101 SYLGAISTMANPLFTPAEVVKQAK 124

The following BLAST results are available for this feature:

BLAST of EY684494 vs. ExPASy Swiss-Prot

BLAST of EY684494 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 18


Match Name	E-value	Identity	Description
4CL2_SOYBN	7.270e-37	58.62	4-coumarate--CoA ligase 2 OS=Glycine max PE=2 SV=2	[more]
4CL3_ARATH	2.450e-33	50.92	4-coumarate--CoA ligase 3 OS=Arabidopsis thaliana ...	[more]
4CL2_ORYSJ	1.014e-28	48.31	Probable 4-coumarate--CoA ligase 2 OS=Oryza sativa...	[more]
4CL2_ARATH	4.164e-28	54.02	4-coumarate--CoA ligase 2 OS=Arabidopsis thaliana ...	[more]
4CL_PINTA	1.090e-27	55.43	4-coumarate--CoA ligase OS=Pinus taeda GN=4CL PE=2...	[more]
4CL2_PETCR	2.580e-26	54.02	4-coumarate--CoA ligase 1 OS=Petroselinum crispum ...	[more]
4CL1_PETCR	2.580e-26	54.02	4-coumarate--CoA ligase 1 OS=Petroselinum crispum ...	[more]
4CL1_TOBAC	2.125e-25	58.62	4-coumarate--CoA ligase 1 OS=Nicotiana tabacum GN=...	[more]
4CL2_SOLTU	1.033e-24	56.32	4-coumarate--CoA ligase 2 OS=Solanum tuberosum GN=...	[more]
4CL1_SOLTU	1.033e-24	56.32	4-coumarate--CoA ligase 1 OS=Solanum tuberosum GN=...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C1-650-055-F11-CT.F Sweet orange leaf, young greenhouse plant Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY684494 ID=EY684494; Name=EY684494; organism=Citrus sinensis; type=EST; length=921bp
CTTAATTTTCCCTAAACAATAGCCAGCTCCTCCTACTCATCTAAAAAAAT
TTCGACAAAACAAAAAAGGAACTGACGATGATATCAATAGCCACTAAAAA
ACCAGAGCTGTCTCTTGATATCTCTTCAGCAGCACCACCAGCGCCGTCCA
ATGAAAAGATAGCCACCCATATTTTCAAATCAAAGCTTCCCGATATACCC
ATCTCCAATCATCTCCCTCTCCATACATATTGTTTCCAGGATCGTTTATC
TGACGATCCATGCTTAATCGTTGGCTCAACTGGCAAAACTTACTCTTATG
CCGAAACGCACCTCATCTGTCGAAAAACCGCAGCCGGTTTGTCGAATCTC
GGCATCAAAAAGGGCGACGTGATCATGATCCTCCTCCAGAACTGCGCCGA
ATTCGTCTTCTCGTTCATGGGAAGCTTTCCATGATCGGTGCAGTCACGAC
GACGGCCAATCCCTTCTACACTTCAGCTGAAATCTTGAAACAGTTCAAAA
ACCTCTGGGGGCCAAATTGATTAATCACATTGGTCCCCGTAAGTCAAAGG
GGTTGCCAAAAGACGGAACAAGAATTTCCACAATGGATTCCGATCGATGC
CCCCCCGGAAAAATTTGCCGCATTTCCCAGGGTTATCCGGGAAGCCAAAT
GAGGATCAATCCCCAAAAATTGGCATCCAGGCCCGGAAAGAAACCGGGGG
GGTTGCCCTTCTTTTCGGGGACAAAGGGATTGGCCTAAAGGGGGGGGGGG
GTGAAACCCAGAAAACTTTGTTACCACAAGGGGGGCCGAAAAGGGAATGG
GAAAAAAACCAAAAAATCTTTTTTGTGAGGAGCGGGAGAGTATGGGGGGG
GGGCGTTTTTCATTTTGTTTTACCAAATTTATTTTTATTTAAAACAAGGA
GGGTTTTTGGAGGTCTCTCTC