EY684277 | Citrus Genome Database

Overview


Name	EY684277
Unique Name	EY684277
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	934

Libraries


Library Name	Type
Sweet orange leaf, young greenhouse plant	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY684277 vs. ExPASy Swiss-Prot
Match: TCMO_POPKI (Trans-cinnamate 4-monooxygenase OS=Populus kitakamiensis GN=CYP73A16 PE=2 SV=1)

HSP 1 Score: 50.0618 bits (118), Expect = 1.535e-14
Identity = 23/29 (79.31%), Postives = 26/29 (89.66%), Query Frame = 1

Query:  469 RKLASTKSMSNESLKCANDHILDAQNKGK 555
            +KLASTK+MSNE LKCA DHILDAQ KG+
Sbjct:  262 KKLASTKNMSNEGLKCAIDHILDAQKKGE 290

HSP 2 Score: 43.5134 bits (101), Expect = 1.535e-14
Identity = 20/32 (62.50%), Postives = 24/32 (75.00%), Query Frame = 3

Query:  585 IKNIKVA*IEKTLWAIEWGICKLGNPPENPKK 680
            ++NI VA IE TLW+IEWGI +L N PE  KK
Sbjct:  300 VENINVAAIETTLWSIEWGIAELVNHPEIQKK 331

HSP 3 Score: 26.5646 bits (57), Expect = 1.535e-14
Identity = 10/12 (83.33%), Postives = 12/12 (100.00%), Query Frame = 2

Query:  548 KGKINEDNILYI 583
            KG+INEDN+LYI
Sbjct:  288 KGEINEDNVLYI 299

[Close]

BLAST of EY684277 vs. ExPASy Swiss-Prot
Match: TCMO_POPTM (Trans-cinnamate 4-monooxygenase OS=Populus tremuloides GN=CYP73A13 PE=2 SV=1)

HSP 1 Score: 48.1358 bits (113), Expect = 5.557e-14
Identity = 22/29 (75.86%), Postives = 25/29 (86.21%), Query Frame = 1

Query:  469 RKLASTKSMSNESLKCANDHILDAQNKGK 555
            +KLASTK+M NE LKCA DHILDAQ KG+
Sbjct:  262 KKLASTKNMCNEGLKCAIDHILDAQKKGE 290

HSP 2 Score: 43.5134 bits (101), Expect = 5.557e-14
Identity = 20/32 (62.50%), Postives = 24/32 (75.00%), Query Frame = 3

Query:  585 IKNIKVA*IEKTLWAIEWGICKLGNPPENPKK 680
            ++NI VA IE TLW+IEWGI +L N PE  KK
Sbjct:  300 VENINVAAIETTLWSIEWGIAELVNHPEIQKK 331

HSP 3 Score: 26.5646 bits (57), Expect = 5.557e-14
Identity = 10/12 (83.33%), Postives = 12/12 (100.00%), Query Frame = 2

Query:  548 KGKINEDNILYI 583
            KG+INEDN+LYI
Sbjct:  288 KGEINEDNVLYI 299

[Close]

BLAST of EY684277 vs. ExPASy Swiss-Prot
Match: TCMO_GLYEC (Trans-cinnamate 4-monooxygenase OS=Glycyrrhiza echinata GN=CYP73A14 PE=2 SV=1)

HSP 1 Score: 46.2098 bits (108), Expect = 1.554e-13
Identity = 22/28 (78.57%), Postives = 23/28 (82.14%), Query Frame = 1

Query:  472 KLASTKSMSNESLKCANDHILDAQNKGK 555
            KL STKS SNE LKCA DHILDAQ KG+
Sbjct:  263 KLESTKSTSNEGLKCAIDHILDAQKKGE 290

HSP 2 Score: 43.8986 bits (102), Expect = 1.554e-13
Identity = 24/52 (46.15%), Postives = 29/52 (55.77%), Query Frame = 3

Query:  585 IKNIKVA*IEKTLWAIEWGICKLGNPPENPKKXXXXXXXXXXXXXXHILGPG 740
            ++NI VA IE TLW+IEWGI +L N PE  KK               +LGPG
Sbjct:  300 VENINVAAIETTLWSIEWGIAELVNHPEIQKK--------VRDEIDRVLGPG 343

HSP 3 Score: 26.5646 bits (57), Expect = 1.554e-13
Identity = 10/12 (83.33%), Postives = 12/12 (100.00%), Query Frame = 2

Query:  548 KGKINEDNILYI 583
            KG+INEDN+LYI
Sbjct:  288 KGEINEDNVLYI 299

[Close]

BLAST of EY684277 vs. ExPASy Swiss-Prot
Match: TCMO_CATRO (Trans-cinnamate 4-monooxygenase OS=Catharanthus roseus GN=CYP73A4 PE=2 SV=1)

HSP 1 Score: 44.669 bits (104), Expect = 5.614e-13
Identity = 20/29 (68.97%), Postives = 23/29 (79.31%), Query Frame = 1

Query:  469 RKLASTKSMSNESLKCANDHILDAQNKGK 555
            +K  STKSM N SLKCA DHIL+AQ KG+
Sbjct:  262 KKFGSTKSMDNNSLKCAIDHILEAQQKGE 290

HSP 2 Score: 43.5134 bits (101), Expect = 5.614e-13
Identity = 20/32 (62.50%), Postives = 24/32 (75.00%), Query Frame = 3

Query:  585 IKNIKVA*IEKTLWAIEWGICKLGNPPENPKK 680
            ++NI VA IE TLW+IEWGI +L N PE  KK
Sbjct:  300 VENINVAAIETTLWSIEWGIAELVNHPEIQKK 331

HSP 3 Score: 26.5646 bits (57), Expect = 5.614e-13
Identity = 10/12 (83.33%), Postives = 12/12 (100.00%), Query Frame = 2

Query:  548 KGKINEDNILYI 583
            KG+INEDN+LYI
Sbjct:  288 KGEINEDNVLYI 299

[Close]

BLAST of EY684277 vs. ExPASy Swiss-Prot
Match: TCMO_RUTGR (Trans-cinnamate 4-monooxygenase OS=Ruta graveolens GN=CYP73A2 PE=2 SV=1)

HSP 1 Score: 44.669 bits (104), Expect = 1.212e-12
Identity = 19/29 (65.52%), Postives = 24/29 (82.76%), Query Frame = 1

Query:  469 RKLASTKSMSNESLKCANDHILDAQNKGK 555
            +KL STKSM+ E+ KCA DH+LDAQ KG+
Sbjct:  262 KKLTSTKSMTEENFKCAIDHVLDAQQKGE 290

HSP 2 Score: 42.3578 bits (98), Expect = 1.212e-12
Identity = 19/32 (59.38%), Postives = 24/32 (75.00%), Query Frame = 3

Query:  585 IKNIKVA*IEKTLWAIEWGICKLGNPPENPKK 680
            ++NI VA IE TLW+IEWGI +L N P+  KK
Sbjct:  300 VENINVAAIETTLWSIEWGIAELVNHPDIQKK 331

HSP 3 Score: 26.5646 bits (57), Expect = 1.212e-12
Identity = 10/12 (83.33%), Postives = 12/12 (100.00%), Query Frame = 2

Query:  548 KGKINEDNILYI 583
            KG+INEDN+LYI
Sbjct:  288 KGEINEDNVLYI 299

[Close]

BLAST of EY684277 vs. ExPASy Swiss-Prot
Match: TCMO_PETCR (Trans-cinnamate 4-monooxygenase OS=Petroselinum crispum GN=CYP73A10 PE=2 SV=1)

HSP 1 Score: 43.5134 bits (101), Expect = 5.646e-12
Identity = 20/32 (62.50%), Postives = 24/32 (75.00%), Query Frame = 3

Query:  585 IKNIKVA*IEKTLWAIEWGICKLGNPPENPKK 680
            ++NI VA IE TLW+IEWGI +L N PE  KK
Sbjct:  301 VENINVAAIETTLWSIEWGIAELVNNPEIQKK 332

HSP 2 Score: 41.2022 bits (95), Expect = 5.646e-12
Identity = 18/29 (62.07%), Postives = 23/29 (79.31%), Query Frame = 1

Query:  469 RKLASTKSMSNESLKCANDHILDAQNKGK 555
            +KL S KS+ N SLKCA DHI++AQ KG+
Sbjct:  263 KKLESIKSVDNNSLKCAIDHIIEAQQKGE 291

HSP 3 Score: 26.5646 bits (57), Expect = 5.646e-12
Identity = 10/12 (83.33%), Postives = 12/12 (100.00%), Query Frame = 2

Query:  548 KGKINEDNILYI 583
            KG+INEDN+LYI
Sbjct:  289 KGEINEDNVLYI 300

[Close]

BLAST of EY684277 vs. ExPASy Swiss-Prot
Match: TCMO_ZINEL (Trans-cinnamate 4-monooxygenase OS=Zinnia elegans GN=CYP73A12 PE=2 SV=1)

HSP 1 Score: 45.0542 bits (105), Expect = 7.297e-12
Identity = 20/29 (68.97%), Postives = 24/29 (82.76%), Query Frame = 1

Query:  469 RKLASTKSMSNESLKCANDHILDAQNKGK 555
            +KL STKSM N  LKCA DHILDA++KG+
Sbjct:  262 KKLGSTKSMDNNQLKCAIDHILDAKDKGE 290

HSP 2 Score: 38.891 bits (89), Expect = 7.297e-12
Identity = 17/28 (60.71%), Postives = 21/28 (75.00%), Query Frame = 3

Query:  585 IKNIKVA*IEKTLWAIEWGICKLGNPPE 668
            ++NI VA IE TLW+IEW I +L N PE
Sbjct:  300 VENINVAAIETTLWSIEWAIAELVNHPE 327

HSP 3 Score: 26.9498 bits (58), Expect = 7.297e-12
Identity = 10/14 (71.43%), Postives = 13/14 (92.86%), Query Frame = 2

Query:  542 RTKGKINEDNILYI 583
            + KG+INEDN+LYI
Sbjct:  286 KDKGEINEDNVLYI 299

[Close]

BLAST of EY684277 vs. ExPASy Swiss-Prot
Match: TCMO_PHAAU (Trans-cinnamate 4-monooxygenase OS=Phaseolus aureus GN=CYP73A2 PE=1 SV=1)

HSP 1 Score: 42.3578 bits (98), Expect = 7.297e-12
Identity = 18/29 (62.07%), Postives = 23/29 (79.31%), Query Frame = 1

Query:  469 RKLASTKSMSNESLKCANDHILDAQNKGK 555
            + + STKS +NE LKCA DHILDA+ KG+
Sbjct:  262 KNIGSTKSTNNEGLKCAIDHILDAEKKGE 290

HSP 2 Score: 41.9726 bits (97), Expect = 7.297e-12
Identity = 19/32 (59.38%), Postives = 24/32 (75.00%), Query Frame = 3

Query:  585 IKNIKVA*IEKTLWAIEWGICKLGNPPENPKK 680
            ++NI VA IE TLW+IEWGI +L N PE  +K
Sbjct:  300 VENINVAAIETTLWSIEWGIAELVNHPEIQQK 331

HSP 3 Score: 26.5646 bits (57), Expect = 7.297e-12
Identity = 10/12 (83.33%), Postives = 12/12 (100.00%), Query Frame = 2

Query:  548 KGKINEDNILYI 583
            KG+INEDN+LYI
Sbjct:  288 KGEINEDNVLYI 299

[Close]

BLAST of EY684277 vs. ExPASy Swiss-Prot
Match: TCMO_CICAR (Trans-cinnamate 4-monooxygenase OS=Cicer arietinum GN=CYP73A19 PE=2 SV=2)

HSP 1 Score: 46.2098 bits (108), Expect = 2.625e-11
Identity = 21/29 (72.41%), Postives = 24/29 (82.76%), Query Frame = 1

Query:  469 RKLASTKSMSNESLKCANDHILDAQNKGK 555
            +KL STKS +NE LKCA DHILDAQ KG+
Sbjct:  261 KKLGSTKSTTNEGLKCAIDHILDAQQKGE 289

HSP 2 Score: 37.3502 bits (85), Expect = 2.625e-11
Identity = 16/25 (64.00%), Postives = 20/25 (80.00%), Query Frame = 3

Query:  585 IKNIKVA*IEKTLWAIEWGICKLGN 659
            ++NI VA IE TLW+IEWGI +L N
Sbjct:  299 VENINVAAIETTLWSIEWGIAELVN 323

HSP 3 Score: 25.409 bits (54), Expect = 2.625e-11
Identity = 9/12 (75.00%), Postives = 12/12 (100.00%), Query Frame = 2

Query:  548 KGKINEDNILYI 583
            KG+IN+DN+LYI
Sbjct:  287 KGEINDDNVLYI 298

[Close]

BLAST of EY684277 vs. ExPASy Swiss-Prot
Match: TCMO_PEA (Trans-cinnamate 4-monooxygenase OS=Pisum sativum GN=CYP73A9 PE=2 SV=2)

HSP 1 Score: 45.0542 bits (105), Expect = 2.625e-11
Identity = 21/29 (72.41%), Postives = 23/29 (79.31%), Query Frame = 1

Query:  469 RKLASTKSMSNESLKCANDHILDAQNKGK 555
            +KL STKS  NE LKCA DHILDAQ KG+
Sbjct:  262 KKLGSTKSTYNEGLKCAIDHILDAQKKGE 290

HSP 2 Score: 38.5058 bits (88), Expect = 2.625e-11
Identity = 18/32 (56.25%), Postives = 22/32 (68.75%), Query Frame = 3

Query:  585 IKNIKVA*IEKTLWAIEWGICKLGNPPENPKK 680
            ++NI VA IE TLW+IEWGI +L N  E   K
Sbjct:  300 VENINVAAIETTLWSIEWGIAELVNHQEIQNK 331

HSP 3 Score: 25.409 bits (54), Expect = 2.625e-11
Identity = 9/12 (75.00%), Postives = 12/12 (100.00%), Query Frame = 2

Query:  548 KGKINEDNILYI 583
            KG+IN+DN+LYI
Sbjct:  288 KGEINDDNVLYI 299

The following BLAST results are available for this feature:

BLAST of EY684277 vs. ExPASy Swiss-Prot

BLAST of EY684277 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 12


Match Name	E-value	Identity	Description
TCMO_POPKI	1.535e-14	79.31	Trans-cinnamate 4-monooxygenase OS=Populus kitakam...	[more]
TCMO_POPTM	5.557e-14	75.86	Trans-cinnamate 4-monooxygenase OS=Populus tremulo...	[more]
TCMO_GLYEC	1.554e-13	78.57	Trans-cinnamate 4-monooxygenase OS=Glycyrrhiza ech...	[more]
TCMO_CATRO	5.614e-13	68.97	Trans-cinnamate 4-monooxygenase OS=Catharanthus ro...	[more]
TCMO_RUTGR	1.212e-12	65.52	Trans-cinnamate 4-monooxygenase OS=Ruta graveolens...	[more]
TCMO_PETCR	5.646e-12	62.50	Trans-cinnamate 4-monooxygenase OS=Petroselinum cr...	[more]
TCMO_ZINEL	7.297e-12	68.97	Trans-cinnamate 4-monooxygenase OS=Zinnia elegans ...	[more]
TCMO_PHAAU	7.297e-12	62.07	Trans-cinnamate 4-monooxygenase OS=Phaseolus aureu...	[more]
TCMO_CICAR	2.625e-11	72.41	Trans-cinnamate 4-monooxygenase OS=Cicer arietinum...	[more]
TCMO_PEA	2.625e-11	72.41	Trans-cinnamate 4-monooxygenase OS=Pisum sativum G...	[more]

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Properties


Property Name	Value
Genbank description	CS00-C1-650-051-B08-CT.F Sweet orange leaf, young greenhouse plant Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY684277 ID=EY684277; Name=EY684277; organism=Citrus sinensis; type=EST; length=934bp
TGAAAGAGAGAAGGAGGACGGGTCCGGAAATCCGAGTCGACCCCCGCGTC
CGATTCAACTTTATTTGTGGGATTATGATCTCTCATTTTTCTGGTCTTTT
TTTGTTTTTAAATAAATAAAAGTAAAAATTAATATAATTGTGTATTTTAA
AATTTAAATAATTTTTTTACGAAAAAAAAAGAAATAATAATCAACGGTGG
GGGCGTCGTAATCAATTAATGACATAATAATTATTTTTAAAATAAATAAA
TATTCCGTGACCGTTTTGTCTAACACATTCTCCTTATCCCTTTGCTTCGA
CGAGGATCTAGTTTCTGTCGGTTTCTAGTTATGTTTTGGAAACAAAATCA
GCGGCCGTGCTGCCCATGGCCCAACGAAAGAATTGCTTACGTTGACATTT
ATTTTTATTGTAGATATTCTTATTGCCGTCATTTTATTATTCAATTGACG
TGATTAATATTTATTGGCAGGAAATTGGCAAGCACGAAGAGCATGAGTAA
TGAAAGCTTGAAATGCGCAAACGATCATATTTTGGACGCTCAGAACAAAG
GGAAAAATCAACGAGGACAACATTCTATACATCGATTAAAAACATCAAGG
TTGCCTAAATCGAAAAAACTCTTTGGGCAATCGAATGGGGAATTTGCAAA
CTAGGGAATCCTCCCGAAAATCCCAAAAAAACTCCCAATGGAAACTGAAC
ACCGGGCTTGGGTCCTGGTACTCACATTCTTGGACCTGGAACCCCCAAAT
TAACTCAACATTTAGGGCAGTGAACAAGGGAAAATTCCTCCCCTTAAAAA
AGGAAAATTCCTTTTTATTGTCCCCCAAATGAAACAATTCACAAACCCAA
AAGTGGGAAGGGTCAAAACACCCCTGGGCGAAAAAAAAAAAATTCTTATG
AGGAAACGGGGGGAGGACGGACCCGAAATACCAC