EY684243

Overview
NameEY684243
Unique NameEY684243
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length934
Libraries
Library NameType
Sweet orange leaf, young greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY684243 vs. ExPASy Swiss-Prot
Match: PAPOA_HUMAN (Poly(A) polymerase alpha OS=Homo sapiens GN=PAPOLA PE=1 SV=4)

HSP 1 Score: 181.8 bits (460), Expect = 1.051e-48
Identity = 89/151 (58.94%), Postives = 106/151 (70.20%), Query Frame = 3 
Query:  180 LQNFRTTLRCLKFWAKRRGIYSNANGFLGGVNWALLVARVCQLYPNAIPSMLVSRFFRVYTQWRWPNPVMMCPIEMGELGFSVWDPRKNPKDRSHRMPIITPAYHCMNSSYKVSLGTLQVMTEQFQCGNRICEEIGLGQAE*SALFEPLSF 632
            + NFR TLR +K WAKR  IYSN  GFLGGV+WA+LVAR CQLYPNAI S LV +FF V+++W WPNPV++   E   L   VWDPR NP DR H MPIITPAY   NS+Y VS+ T  VM E+F+ G  I +EI L +AE S LFE  +F
Sbjct:  217 IDNFRLTLRAIKLWAKRHNIYSNILGFLGGVSWAMLVARTCQLYPNAIASTLVHKFFLVFSKWEWPNPVLLKQPEECNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSVSTRMVMVEEFKQGLAITDEILLSKAEWSKLFEAPNF 367

HSP 2 Score: 33.8834 bits (76), Expect = 1.051e-48
Identity = 15/31 (48.39%), Postives = 23/31 (74.19%), Query Frame = 1 
Query:   13 SVLFVIDKQTVQSLNGCWVADQILKHVPNVE 105
            S+L  +D + ++SLNGC V D+IL  VPN++
Sbjct:  188 SLLKNLDIRCIRSLNGCRVTDEILHLVPNID 218
[Close]
BLAST of EY684243 vs. ExPASy Swiss-Prot
Match: PAPOA_MOUSE (Poly(A) polymerase alpha OS=Mus musculus GN=Papola PE=1 SV=4)

HSP 1 Score: 181.8 bits (460), Expect = 1.051e-48
Identity = 89/151 (58.94%), Postives = 106/151 (70.20%), Query Frame = 3 
Query:  180 LQNFRTTLRCLKFWAKRRGIYSNANGFLGGVNWALLVARVCQLYPNAIPSMLVSRFFRVYTQWRWPNPVMMCPIEMGELGFSVWDPRKNPKDRSHRMPIITPAYHCMNSSYKVSLGTLQVMTEQFQCGNRICEEIGLGQAE*SALFEPLSF 632
            + NFR TLR +K WAKR  IYSN  GFLGGV+WA+LVAR CQLYPNAI S LV +FF V+++W WPNPV++   E   L   VWDPR NP DR H MPIITPAY   NS+Y VS+ T  VM E+F+ G  I +EI L +AE S LFE  +F
Sbjct:  217 IDNFRLTLRAIKLWAKRHNIYSNILGFLGGVSWAMLVARTCQLYPNAIASTLVHKFFLVFSKWEWPNPVLLKQPEECNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSVSTRMVMVEEFKQGLAITDEILLSKAEWSKLFEAPNF 367

HSP 2 Score: 33.8834 bits (76), Expect = 1.051e-48
Identity = 15/31 (48.39%), Postives = 23/31 (74.19%), Query Frame = 1 
Query:   13 SVLFVIDKQTVQSLNGCWVADQILKHVPNVE 105
            S+L  +D + ++SLNGC V D+IL  VPN++
Sbjct:  188 SLLKNLDIRCIRSLNGCRVTDEILHLVPNID 218
[Close]
BLAST of EY684243 vs. ExPASy Swiss-Prot
Match: PAPOA_BOVIN (Poly(A) polymerase alpha OS=Bos taurus GN=PAPOLA PE=1 SV=3)

HSP 1 Score: 181.8 bits (460), Expect = 1.051e-48
Identity = 89/151 (58.94%), Postives = 106/151 (70.20%), Query Frame = 3 
Query:  180 LQNFRTTLRCLKFWAKRRGIYSNANGFLGGVNWALLVARVCQLYPNAIPSMLVSRFFRVYTQWRWPNPVMMCPIEMGELGFSVWDPRKNPKDRSHRMPIITPAYHCMNSSYKVSLGTLQVMTEQFQCGNRICEEIGLGQAE*SALFEPLSF 632
            + NFR TLR +K WAKR  IYSN  GFLGGV+WA+LVAR CQLYPNAI S LV +FF V+++W WPNPV++   E   L   VWDPR NP DR H MPIITPAY   NS+Y VS+ T  VM E+F+ G  I +EI L +AE S LFE  +F
Sbjct:  217 IDNFRLTLRAIKLWAKRHNIYSNILGFLGGVSWAMLVARTCQLYPNAIASTLVHKFFLVFSKWEWPNPVLLKQPEECNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSVSTRMVMVEEFKQGLAITDEILLSKAEWSKLFEAPNF 367

HSP 2 Score: 33.8834 bits (76), Expect = 1.051e-48
Identity = 15/31 (48.39%), Postives = 23/31 (74.19%), Query Frame = 1 
Query:   13 SVLFVIDKQTVQSLNGCWVADQILKHVPNVE 105
            S+L  +D + ++SLNGC V D+IL  VPN++
Sbjct:  188 SLLKNLDIRCIRSLNGCRVTDEILHLVPNID 218
[Close]
BLAST of EY684243 vs. ExPASy Swiss-Prot
Match: PAPO1_XENLA (Poly(A) polymerase alpha-A (Fragment) OS=Xenopus laevis GN=papola-A PE=2 SV=1)

HSP 1 Score: 180.259 bits (456), Expect = 3.034e-48
Identity = 88/151 (58.28%), Postives = 107/151 (70.86%), Query Frame = 3 
Query:  180 LQNFRTTLRCLKFWAKRRGIYSNANGFLGGVNWALLVARVCQLYPNAIPSMLVSRFFRVYTQWRWPNPVMMCPIEMGELGFSVWDPRKNPKDRSHRMPIITPAYHCMNSSYKVSLGTLQVMTEQFQCGNRICEEIGLGQAE*SALFEPLSF 632
            + +FR TLR +K WAKR  IYSN  GFLGGV+WA+LVAR CQLYPNAI S LV +FF V+++W WPNPV++   E   L   VWDPR NP DR H MPIITPAY   NS+Y VS+ T  VM E+F+ G  I +EI LG+AE S LF+  +F
Sbjct:  199 IDSFRLTLRAIKLWAKRHNIYSNILGFLGGVSWAMLVARTCQLYPNAIASTLVHKFFLVFSKWEWPNPVLLKQPEECNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSVSTRAVMIEEFKQGLAITDEILLGKAEWSKLFDAPNF 349

HSP 2 Score: 33.8834 bits (76), Expect = 3.034e-48
Identity = 15/31 (48.39%), Postives = 23/31 (74.19%), Query Frame = 1 
Query:   13 SVLFVIDKQTVQSLNGCWVADQILKHVPNVE 105
            S+L  +D + ++SLNGC V D+IL  VPN++
Sbjct:  170 SLLKNLDIRCIRSLNGCRVTDEILHLVPNID 200
[Close]
BLAST of EY684243 vs. ExPASy Swiss-Prot
Match: PAPOB_HUMAN (Poly(A) polymerase beta OS=Homo sapiens GN=PAPOLB PE=2 SV=1)

HSP 1 Score: 179.489 bits (454), Expect = 5.159e-48
Identity = 88/151 (58.28%), Postives = 104/151 (68.87%), Query Frame = 3 
Query:  180 LQNFRTTLRCLKFWAKRRGIYSNANGFLGGVNWALLVARVCQLYPNAIPSMLVSRFFRVYTQWRWPNPVMMCPIEMGELGFSVWDPRKNPKDRSHRMPIITPAYHCMNSSYKVSLGTLQVMTEQFQCGNRICEEIGLGQAE*SALFEPLSF 632
            + NFR TLR +K WAK   IYSN  GFLGGV+WA+LVAR CQLYPNA+ S LV +FF V+++W WPNPV++   E   L   VWDPR NP DR H MPIITPAY   NS+Y VS+ T  VM E+F+ G  I  EI L +AE S LFE  SF
Sbjct:  217 IDNFRLTLRAIKLWAKCHNIYSNILGFLGGVSWAMLVARTCQLYPNAVASTLVRKFFLVFSEWEWPNPVLLKEPEERNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSISTRMVMIEEFKQGLAITHEILLSKAEWSKLFEAPSF 367

HSP 2 Score: 33.8834 bits (76), Expect = 5.159e-48
Identity = 15/31 (48.39%), Postives = 23/31 (74.19%), Query Frame = 1 
Query:   13 SVLFVIDKQTVQSLNGCWVADQILKHVPNVE 105
            S+L  +D + ++SLNGC V D+IL  VPN++
Sbjct:  188 SLLKNLDIRCIRSLNGCRVTDEILHLVPNID 218
[Close]
BLAST of EY684243 vs. ExPASy Swiss-Prot
Match: PAPOG_MOUSE (Poly(A) polymerase gamma OS=Mus musculus GN=Papolg PE=1 SV=1)

HSP 1 Score: 179.489 bits (454), Expect = 1.938e-47
Identity = 86/150 (57.33%), Postives = 105/150 (70.00%), Query Frame = 3 
Query:  183 QNFRTTLRCLKFWAKRRGIYSNANGFLGGVNWALLVARVCQLYPNAIPSMLVSRFFRVYTQWRWPNPVMMCPIEMGELGFSVWDPRKNPKDRSHRMPIITPAYHCMNSSYKVSLGTLQVMTEQFQCGNRICEEIGLGQAE*SALFEPLSF 632
            + FR TLR +K WAKRRGIYSN  GFLGGV+WA+LVAR CQLYPNA  S LV +FF V+++W WPNPV++   E   L   VWDPR NP DR H MPIITPAY   NS+Y VS  T  VM E+F+ G  + +EI  G+++ S L EP +F
Sbjct:  217 ETFRLTLRAVKLWAKRRGIYSNMLGFLGGVSWAMLVARTCQLYPNAAASTLVHKFFLVFSKWEWPNPVLLKQPEESNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSTSTRTVMVEEFKQGLAVTDEILQGKSDWSKLLEPPNF 366

HSP 2 Score: 31.9574 bits (71), Expect = 1.938e-47
Identity = 14/26 (53.85%), Postives = 19/26 (73.08%), Query Frame = 1 
Query:   28 IDKQTVQSLNGCWVADQILKHVPNVE 105
            +D + ++SLNGC V D+IL  VPN E
Sbjct:  192 LDIRCIRSLNGCRVTDEILHLVPNKE 217
[Close]
BLAST of EY684243 vs. ExPASy Swiss-Prot
Match: PAPOG_HUMAN (Poly(A) polymerase gamma OS=Homo sapiens GN=PAPOLG PE=1 SV=2)

HSP 1 Score: 179.489 bits (454), Expect = 1.938e-47
Identity = 86/150 (57.33%), Postives = 105/150 (70.00%), Query Frame = 3 
Query:  183 QNFRTTLRCLKFWAKRRGIYSNANGFLGGVNWALLVARVCQLYPNAIPSMLVSRFFRVYTQWRWPNPVMMCPIEMGELGFSVWDPRKNPKDRSHRMPIITPAYHCMNSSYKVSLGTLQVMTEQFQCGNRICEEIGLGQAE*SALFEPLSF 632
            + FR TLR +K WAKRRGIYSN  GFLGGV+WA+LVAR CQLYPNA  S LV +FF V+++W WPNPV++   E   L   VWDPR NP DR H MPIITPAY   NS+Y VS  T  VM E+F+ G  + +EI  G+++ S L EP +F
Sbjct:  217 ETFRLTLRAVKLWAKRRGIYSNMLGFLGGVSWAMLVARTCQLYPNAAASTLVHKFFLVFSKWEWPNPVLLKQPEESNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSTSTRTVMVEEFKQGLAVTDEILQGKSDWSKLLEPPNF 366

HSP 2 Score: 31.9574 bits (71), Expect = 1.938e-47
Identity = 14/26 (53.85%), Postives = 19/26 (73.08%), Query Frame = 1 
Query:   28 IDKQTVQSLNGCWVADQILKHVPNVE 105
            +D + ++SLNGC V D+IL  VPN E
Sbjct:  192 LDIRCIRSLNGCRVTDEILHLVPNKE 217
[Close]
BLAST of EY684243 vs. ExPASy Swiss-Prot
Match: PAPOB_MOUSE (Poly(A) polymerase beta OS=Mus musculus GN=Papolb PE=1 SV=2)

HSP 1 Score: 177.563 bits (449), Expect = 1.940e-47
Identity = 88/151 (58.28%), Postives = 104/151 (68.87%), Query Frame = 3 
Query:  180 LQNFRTTLRCLKFWAKRRGIYSNANGFLGGVNWALLVARVCQLYPNAIPSMLVSRFFRVYTQWRWPNPVMMCPIEMGELGFSVWDPRKNPKDRSHRMPIITPAYHCMNSSYKVSLGTLQVMTEQFQCGNRICEEIGLGQAE*SALFEPLSF 632
            + +FR TLR +K WAK   IYSN  GFLGGV+WA+LVAR CQLYPNAI S LV +FF V+++W WPNPV++   E   L   VWDPR NP DR H MPIITPAY   NS+Y VS+ T  VM E+F+ G  I  EI L +AE S LFE  SF
Sbjct:  217 IDSFRLTLRAIKLWAKCHNIYSNILGFLGGVSWAMLVARTCQLYPNAIASTLVRKFFLVFSEWEWPNPVLLKEPEERNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSVSTRMVMIEEFKQGLAITHEILLNKAEWSKLFEAPSF 367

HSP 2 Score: 33.8834 bits (76), Expect = 1.940e-47
Identity = 15/31 (48.39%), Postives = 23/31 (74.19%), Query Frame = 1 
Query:   13 SVLFVIDKQTVQSLNGCWVADQILKHVPNVE 105
            S+L  +D + ++SLNGC V D+IL  VPN++
Sbjct:  188 SLLKNLDIRCIRSLNGCRVTDEILHLVPNID 218
[Close]
BLAST of EY684243 vs. ExPASy Swiss-Prot
Match: PAPO3_XENLA (Poly(A) polymerase type 3 (Fragment) OS=Xenopus laevis PE=2 SV=1)

HSP 1 Score: 176.792 bits (447), Expect = 3.312e-47
Identity = 87/151 (57.62%), Postives = 106/151 (70.20%), Query Frame = 3 
Query:  180 LQNFRTTLRCLKFWAKRRGIYSNANGFLGGVNWALLVARVCQLYPNAIPSMLVSRFFRVYTQWRWPNPVMMCPIEMGELGFSVWDPRKNPKDRSHRMPIITPAYHCMNSSYKVSLGTLQVMTEQFQCGNRICEEIGLGQAE*SALFEPLSF 632
            + +FR TLR +K WAKR  IYSN  GFLGGV+WA+LVAR CQLYPNAI S LV +FF V+++W WPNPV++   E   L   VWDPR NP DR H MPIITPAY   NS+Y VS+ T  VM E+F+ G  I +EI L +AE S LF+  +F
Sbjct:  214 IDSFRLTLRAIKLWAKRHNIYSNILGFLGGVSWAMLVARTCQLYPNAIASTLVHKFFLVFSKWEWPNPVLLKQPEECNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSVSTRAVMVEEFKQGLAITDEILLLKAEWSKLFDAPNF 364

HSP 2 Score: 33.8834 bits (76), Expect = 3.312e-47
Identity = 15/31 (48.39%), Postives = 23/31 (74.19%), Query Frame = 1 
Query:   13 SVLFVIDKQTVQSLNGCWVADQILKHVPNVE 105
            S+L  +D + ++SLNGC V D+IL  VPN++
Sbjct:  185 SLLKNLDIRCIRSLNGCRVTDEILHLVPNID 215
[Close]
BLAST of EY684243 vs. ExPASy Swiss-Prot
Match: PAP_DICDI (Poly(A) polymerase OS=Dictyostelium discoideum GN=papA PE=3 SV=1)

HSP 1 Score: 169.474 bits (428), Expect = 3.567e-46
Identity = 82/152 (53.95%), Postives = 104/152 (68.42%), Query Frame = 3 
Query:  180 LQNFRTTLRCLKFWAKRRGIYSNANGFLGGVNWALLVARVCQLYPNAIPSMLVSRFFRVYTQWRWPNPVMMCPI-EMGELGFSVWDPRKNPKDRSHRMPIITPAYHCMNSSYKVSLGTLQVMTEQFQCGNRICEEIGLGQAE*SALFEPLSF 632
            + NFR  LRC+K WA RRGIYSN  GFLGGV+WALL AR+CQLYPN+ PS ++ RFF+VY  W+WP P+++C I E G LG  VW+P+   +D++H MPIITPAY  MNS+Y VS  TLQ+M  +F  G  I  +I  G+     L E   F
Sbjct:  251 IPNFRMALRCIKLWAIRRGIYSNILGFLGGVSWALLTARICQLYPNSAPSTIIHRFFKVYEIWKWPAPILLCHIQEGGILGPKVWNPK---RDKAHLMPIITPAYPSMNSTYNVSKSTLQLMKSEFVRGAEITRKIETGECTWKNLLEKCDF 399

HSP 2 Score: 37.7354 bits (86), Expect = 3.567e-46
Identity = 18/30 (60.00%), Postives = 23/30 (76.67%), Query Frame = 1 
Query:   13 SVLFVIDKQTVQSLNGCWVADQILKHVPNV 102
            S L  ID++++ SLNGC V DQILK VPN+
Sbjct:  222 SFLKNIDEKSILSLNGCRVTDQILKLVPNI 251
The following BLAST results are available for this feature:
BLAST of EY684243 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 15
Match NameE-valueIdentityDescription
PAPOA_HUMAN1.051e-4858.94Poly(A) polymerase alpha OS=Homo sapiens GN=PAPOLA... [more]
PAPOA_MOUSE1.051e-4858.94Poly(A) polymerase alpha OS=Mus musculus GN=Papola... [more]
PAPOA_BOVIN1.051e-4858.94Poly(A) polymerase alpha OS=Bos taurus GN=PAPOLA P... [more]
PAPO1_XENLA3.034e-4858.28Poly(A) polymerase alpha-A (Fragment) OS=Xenopus l... [more]
PAPOB_HUMAN5.159e-4858.28Poly(A) polymerase beta OS=Homo sapiens GN=PAPOLB ... [more]
PAPOG_MOUSE1.938e-4757.33Poly(A) polymerase gamma OS=Mus musculus GN=Papolg... [more]
PAPOG_HUMAN1.938e-4757.33Poly(A) polymerase gamma OS=Homo sapiens GN=PAPOLG... [more]
PAPOB_MOUSE1.940e-4758.28Poly(A) polymerase beta OS=Mus musculus GN=Papolb ... [more]
PAPO3_XENLA3.312e-4757.62Poly(A) polymerase type 3 (Fragment) OS=Xenopus la... [more]
PAP_DICDI3.567e-4653.95Poly(A) polymerase OS=Dictyostelium discoideum GN=... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-650-054-G04-CT.F Sweet orange leaf, young greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY684243 ID=EY684243; Name=EY684243; organism=Citrus sinensis; type=EST; length=934bp
ATTTCGCATATTTCTGTCCTTTTTGTCATTGATAAGCAAACTGTTCAAAG
TCTTAATGGATGCTGGGTTGCAGATCAAATTCTCAAACACGTTCCAAACG
TTGAGGTGAACATATTATGCGCTTCATTTGGTCTTATTTAGTTAACCATT
GGTTTGAACATCCTTTAATCTGTTGTAAGTTGCAGAACTTTCGCACGACG
CTCAGATGTCTGAAGTTCTGGGCTAAAAGGCGCGGCATTTATTCAAACGC
TAATGGATTCCTTGGTGGTGTAAATTGGGCTCTTCTGGTTGCACGCGTGT
GCCAACTTTATCCCAATGCAATTCCCAGTATGCTAGTTTCTCGGTTTTTT
AGAGTGTATACACAGTGGCGTTGGCCAAACCCTGTCATGATGTGTCCAAT
AGAAATGGGTGAACTGGGGTTCTCAGTGTGGGATCCTCGTAAAAACCCCA
AGGACCGATCTCATCGTATGCCTATAATAACTCCAGCATATCATTGTATG
AACTCTAGTTATAAGGTCTCTTTAGGTACTCTTCAAGTTATGACAGAGCA
GTTCCAGTGCGGTAACAGGATCTGTGAGGAAATTGGACTGGGGCAGGCGG
AATGAAGTGCTCTGTTTGAACCTTTATCCTTTCTTTGAGGCATATGAAAA
CTATCTGGAGGGTGATATTGGTGGCACCAAAGGCGGATGATTTAACAACT
TTGGAAAGGGGGGGGGGGAATCCCGGTTTAAAACAACTTACCCCCGGAAA
AAAAAAAACAAAAAAACAAAGGGGGTTGGTGGCAATGGCCCTTCCTTTTC
CCAAATAAAAAACAAAAACCCCCCCCAAAACCCGGGCCCCCATTTTCCGG
CCTTTTTTTTGGGGGGGTTGGGAAAAAAAAAAAAGGGGAGAAAAATAAGA
AAAAAAAAAGTGCCCCCAACTTTTTTGTTTTTTT
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