EY684220 | Citrus Genome Database

Overview


Name	EY684220
Unique Name	EY684220
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	988

Libraries


Library Name	Type
Sweet orange leaf, young greenhouse plant	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY684220 vs. ExPASy Swiss-Prot
Match: MDHC_MAIZE (Malate dehydrogenase, cytoplasmic OS=Zea mays PE=2 SV=2)

HSP 1 Score: 230.72 bits (587), Expect = 3.636e-80
Identity = 116/126 (92.06%), Postives = 120/126 (95.24%), Query Frame = 3

Query:   57 MAPYTVRVLVTGAAGQIGYALVPMIARGVMLGTDQPVILHMLDIPPAAEALNGVKMELVDAAFPLIKGVVATTDAVEACTGVNIAVMVGGFPRKEGMERKDVMSKNVSIYKAQASALEQHAAPNCK 434
            MA   +RVLVTGAAGQIGYALVPMIARGVMLG DQPVILHMLDIPPAAEALNGVKMELVDAAFPL+KGVVATTD VEACTGVN+AVMVGGFPRKEGMERKDVMSKNVSIYK+QASALE HAAPNCK
Sbjct:    1 MAKEPMRVLVTGAAGQIGYALVPMIARGVMLGADQPVILHMLDIPPAAEALNGVKMELVDAAFPLLKGVVATTDVVEACTGVNVAVMVGGFPRKEGMERKDVMSKNVSIYKSQASALEAHAAPNCK 126

HSP 2 Score: 90.1225 bits (222), Expect = 3.636e-80
Identity = 49/70 (70.00%), Postives = 58/70 (82.86%), Query Frame = 1

Query:  505 PSCLFQVLVVANPANTNALILKEFAPSIPAKNITCLTRLNHNRALGQIF*KTEWPNIWMLEM--VIIWGN 708
            P+C  +VLVVANPANTNALILKEFAPSIP KN+TCLTRL+HNRALGQI   +E  N+ + ++  VIIWGN
Sbjct:  123 PNC--KVLVVANPANTNALILKEFAPSIPEKNVTCLTRLDHNRALGQI---SERLNVQVSDVKNVIIWGN 187

[Close]

BLAST of EY684220 vs. ExPASy Swiss-Prot
Match: MDHC2_ARATH (Malate dehydrogenase, cytoplasmic 2 OS=Arabidopsis thaliana GN=At5g43330 PE=2 SV=1)

HSP 1 Score: 229.565 bits (584), Expect = 4.155e-80
Identity = 116/126 (92.06%), Postives = 121/126 (96.03%), Query Frame = 3

Query:   57 MAPYTVRVLVTGAAGQIGYALVPMIARGVMLGTDQPVILHMLDIPPAAEALNGVKMELVDAAFPLIKGVVATTDAVEACTGVNIAVMVGGFPRKEGMERKDVMSKNVSIYKAQASALEQHAAPNCK 434
            MA   VRVLVTGAAGQIGYALVPMIARG+MLG DQPVILHMLDIP AAEALNGVKMELVDAAFPL+KGVVATTDAVEACTGVN+AVMVGGFPRKEGMERKDVMSKNVSIYK+QASALE+HAAPNCK
Sbjct:    1 MAKEPVRVLVTGAAGQIGYALVPMIARGIMLGADQPVILHMLDIPFAAEALNGVKMELVDAAFPLLKGVVATTDAVEACTGVNVAVMVGGFPRKEGMERKDVMSKNVSIYKSQASALEKHAAPNCK 126

HSP 2 Score: 90.8929 bits (224), Expect = 4.155e-80
Identity = 48/68 (70.59%), Postives = 56/68 (82.35%), Query Frame = 1

Query:  505 PSCLFQVLVVANPANTNALILKEFAPSIPAKNITCLTRLNHNRALGQIF*KTEWPNIWMLEMVIIWGN 708
            P+C  +VLVVANPANTNALILKEFAPSIP KNITCLTRL+HNRALGQ+  +   P +  ++ VIIWGN
Sbjct:  123 PNC--KVLVVANPANTNALILKEFAPSIPEKNITCLTRLDHNRALGQVSERLSVP-VSDVKNVIIWGN 187

HSP 3 Score: 21.557 bits (44), Expect = 4.155e-80
Identity = 11/19 (57.89%), Postives = 13/19 (68.42%), Query Frame = 3

Query:  729 PDANLATVKTATWGKSPGR 785
            PD N ATVKT+  G+ P R
Sbjct:  194 PDVNHATVKTSV-GEKPVR 211

[Close]

BLAST of EY684220 vs. ExPASy Swiss-Prot
Match: MDHC1_ARATH (Malate dehydrogenase, cytoplasmic 1 OS=Arabidopsis thaliana GN=At1g04410 PE=1 SV=2)

HSP 1 Score: 230.72 bits (587), Expect = 4.743e-80
Identity = 114/126 (90.48%), Postives = 121/126 (96.03%), Query Frame = 3

Query:   57 MAPYTVRVLVTGAAGQIGYALVPMIARGVMLGTDQPVILHMLDIPPAAEALNGVKMELVDAAFPLIKGVVATTDAVEACTGVNIAVMVGGFPRKEGMERKDVMSKNVSIYKAQASALEQHAAPNCK 434
            MA   VRVLVTGAAGQIGYALVPMIARG+MLG DQPVILHMLDIPPAAEALNGVKMEL+DAAFPL+KGVVATTDAVE CTGVN+AVMVGGFPRKEGMERKDVMSKNVSIYK+QA+ALE+HAAPNCK
Sbjct:    1 MAKEPVRVLVTGAAGQIGYALVPMIARGIMLGADQPVILHMLDIPPAAEALNGVKMELIDAAFPLLKGVVATTDAVEGCTGVNVAVMVGGFPRKEGMERKDVMSKNVSIYKSQAAALEKHAAPNCK 126

HSP 2 Score: 89.7373 bits (221), Expect = 4.743e-80
Identity = 48/68 (70.59%), Postives = 56/68 (82.35%), Query Frame = 1

Query:  505 PSCLFQVLVVANPANTNALILKEFAPSIPAKNITCLTRLNHNRALGQIF*KTEWPNIWMLEMVIIWGN 708
            P+C  +VLVVANPANTNALILKEFAPSIP KNI+CLTRL+HNRALGQI  +   P +  ++ VIIWGN
Sbjct:  123 PNC--KVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLSVP-VSDVKNVIIWGN 187

[Close]

BLAST of EY684220 vs. ExPASy Swiss-Prot
Match: MDHC_MESCR (Malate dehydrogenase, cytoplasmic OS=Mesembryanthemum crystallinum GN=MDH PE=2 SV=1)

HSP 1 Score: 229.18 bits (583), Expect = 2.335e-79
Identity = 115/126 (91.27%), Postives = 120/126 (95.24%), Query Frame = 3

Query:   57 MAPYTVRVLVTGAAGQIGYALVPMIARGVMLGTDQPVILHMLDIPPAAEALNGVKMELVDAAFPLIKGVVATTDAVEACTGVNIAVMVGGFPRKEGMERKDVMSKNVSIYKAQASALEQHAAPNCK 434
            MA   +RVLVTGAAGQIGYALVPMIARG+MLG +QPVILHMLDIPPAAEALNGVKMELVDAAFPL+KGVVATTDA EAC GVN+AVMVGGFPRKEGMERKDVMSKNVSIYKAQASALEQHAAPNCK
Sbjct:    1 MAVEPLRVLVTGAAGQIGYALVPMIARGIMLGANQPVILHMLDIPPAAEALNGVKMELVDAAFPLLKGVVATTDAAEACKGVNVAVMVGGFPRKEGMERKDVMSKNVSIYKAQASALEQHAAPNCK 126

HSP 2 Score: 88.9669 bits (219), Expect = 2.335e-79
Identity = 49/70 (70.00%), Postives = 58/70 (82.86%), Query Frame = 1

Query:  505 PSCLFQVLVVANPANTNALILKEFAPSIPAKNITCLTRLNHNRALGQIF*KTEWPNIWMLEM--VIIWGN 708
            P+C  +VLVVANPANTNALILKEFAPSIP KNI+CLTRL+HNRALGQI   +E  N+ + ++  VIIWGN
Sbjct:  123 PNC--KVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQI---SERLNVQVSDVKNVIIWGN 187

[Close]

BLAST of EY684220 vs. ExPASy Swiss-Prot
Match: MDHC_ORYSJ (Malate dehydrogenase, cytoplasmic OS=Oryza sativa subsp. japonica GN=Os10g0478200 PE=1 SV=3)

HSP 1 Score: 226.483 bits (576), Expect = 3.973e-79
Identity = 112/126 (88.89%), Postives = 119/126 (94.44%), Query Frame = 3

Query:   57 MAPYTVRVLVTGAAGQIGYALVPMIARGVMLGTDQPVILHMLDIPPAAEALNGVKMELVDAAFPLIKGVVATTDAVEACTGVNIAVMVGGFPRKEGMERKDVMSKNVSIYKAQASALEQHAAPNCK 434
            MA   +RVLVTGAAGQIGYALVPMIARGVMLG DQPVILHMLDIPPA E+LNG+KMELVDAAFPL+KG+VATTD VEACTGVN+AVMVGGFPRKEGMERKDVMSKNVSIYK+QASALE HAAPNCK
Sbjct:    1 MAKEPMRVLVTGAAGQIGYALVPMIARGVMLGADQPVILHMLDIPPATESLNGLKMELVDAAFPLLKGIVATTDVVEACTGVNVAVMVGGFPRKEGMERKDVMSKNVSIYKSQASALEAHAAPNCK 126

HSP 2 Score: 90.8929 bits (224), Expect = 3.973e-79
Identity = 61/123 (49.59%), Postives = 75/123 (60.98%), Query Frame = 1

Query:  505 PSCLFQVLVVANPANTNALILKEFAPSIPAKNITCLTRLNHNRALGQIF*KTEWPNIWMLEM--VIIWGNSLFPPLPLMPTWQLSKLPLGEKARAGEPWQGRKAGVKWKNFQTPGPKKRGGVA 867
            P+C  +VLVVANPANTNALILKEFAPSIP KNITCLTRL+HNRALGQI   +E  N+ + ++   IIWGN      P +      K P GEK     P +   A  +W N +     ++ G A
Sbjct:  123 PNC--KVLVVANPANTNALILKEFAPSIPEKNITCLTRLDHNRALGQI---SEKLNVQVTDVKNAIIWGNHSSTQYPDV-NHATVKTPSGEK-----PVRELVADDEWLNTEFISTVQQRGAA 234

[Close]

BLAST of EY684220 vs. ExPASy Swiss-Prot
Match: MDHC_BETVU (Malate dehydrogenase, cytoplasmic OS=Beta vulgaris GN=NR1 PE=1 SV=1)

HSP 1 Score: 222.631 bits (566), Expect = 1.638e-77
Identity = 112/126 (88.89%), Postives = 118/126 (93.65%), Query Frame = 3

Query:   57 MAPYTVRVLVTGAAGQIGYALVPMIARGVMLGTDQPVILHMLDIPPAAEALNGVKMELVDAAFPLIKGVVATTDAVEACTGVNIAVMVGGFPRKEGMERKDVMSKNVSIYKAQASALEQHAAPNCK 434
            MA   +RVLVTGAAGQIGYALVPMIARGVMLG +QPVILHMLDIPPAAEALNGVKMELVDAAFPL+KGVVATTD  EAC GVN+AVMVGGFPRKEGMERKDVM KNVSIYK+QASALEQ+AAPNCK
Sbjct:    1 MAVEPLRVLVTGAAGQIGYALVPMIARGVMLGANQPVILHMLDIPPAAEALNGVKMELVDAAFPLLKGVVATTDVAEACKGVNVAVMVGGFPRKEGMERKDVMPKNVSIYKSQASALEQYAAPNCK 126

HSP 2 Score: 89.3521 bits (220), Expect = 1.638e-77
Identity = 48/68 (70.59%), Postives = 55/68 (80.88%), Query Frame = 1

Query:  505 PSCLFQVLVVANPANTNALILKEFAPSIPAKNITCLTRLNHNRALGQIF*KTEWPNIWMLEMVIIWGN 708
            P+C  +VLVVANPANTNALILKEFAPSIP KNITCLTRL+HNRALGQI  +     +  ++ VIIWGN
Sbjct:  123 PNC--KVLVVANPANTNALILKEFAPSIPEKNITCLTRLDHNRALGQISERLN-AQVSDVKNVIIWGN 187

[Close]

BLAST of EY684220 vs. ExPASy Swiss-Prot
Match: MDHC_MEDSA (Malate dehydrogenase, cytoplasmic OS=Medicago sativa GN=CMDH PE=2 SV=1)

HSP 1 Score: 224.942 bits (572), Expect = 2.787e-77
Identity = 115/126 (91.27%), Postives = 119/126 (94.44%), Query Frame = 3

Query:   57 MAPYTVRVLVTGAAGQIGYALVPMIARGVMLGTDQPVILHMLDIPPAAEALNGVKMELVDAAFPLIKGVVATTDAVEACTGVNIAVMVGGFPRKEGMERKDVMSKNVSIYKAQASALEQHAAPNCK 434
            MA   VRVLVTGAAGQIGYALVPMIARGVMLG DQPVILHMLDI PAAE+LNGVKMELVDAAFPL+KGVVATTD VEACTGVNIAVMVGGFPRKEGMERKDVMSKNVSIYK+QASALE+HAA NCK
Sbjct:    1 MAKDPVRVLVTGAAGQIGYALVPMIARGVMLGPDQPVILHMLDIAPAAESLNGVKMELVDAAFPLLKGVVATTDVVEACTGVNIAVMVGGFPRKEGMERKDVMSKNVSIYKSQASALEKHAAANCK 126

HSP 2 Score: 86.2705 bits (212), Expect = 2.787e-77
Identity = 46/65 (70.77%), Postives = 55/65 (84.62%), Query Frame = 1

Query:  520 QVLVVANPANTNALILKEFAPSIPAKNITCLTRLNHNRALGQIF*KTEWPNIWMLEM--VIIWGN 708
            +VLVVANPANTNALILKEFAPSIP +NI+CLTRL+HNRALGQI   +E  N+ + ++  VIIWGN
Sbjct:  126 KVLVVANPANTNALILKEFAPSIPERNISCLTRLDHNRALGQI---SERLNVQVSDVKNVIIWGN 187

[Close]

BLAST of EY684220 vs. ExPASy Swiss-Prot
Match: MDHC_ECHGR (Malate dehydrogenase, cytoplasmic OS=Echinococcus granulosus GN=MDH PE=2 SV=2)

HSP 1 Score: 129.028 bits (323), Expect = 3.248e-44
Identity = 64/124 (51.61%), Postives = 86/124 (69.35%), Query Frame = 3

Query:   63 PYTVRVLVTGAAGQIGYALVPMIARGVMLGTDQPVILHMLDIPPAAEALNGVKMELVDAAFPLIKGVVATTDAVEACTGVNIAVMVGGFPRKEGMERKDVMSKNVSIYKAQASALEQHAAPNCK 434
            P  +RVL+TGAAGQI Y L  M+A G + G DQ +ILH+LDIP A   L+GV MEL D AF ++ G+V T    EA T +++A+MVG  PRK+GMER+D++S NV I+K Q  AL+++A    K
Sbjct:    2 PGPLRVLITGAAGQIAYNLSNMVANGNLFGKDQQIILHLLDIPEAKTVLDGVVMELQDCAFTVLAGIVPTHCLKEAFTDIDVALMVGAMPRKQGMERRDLLSSNVKIFKEQGEALDKYAKKTVK 125

HSP 2 Score: 71.633 bits (174), Expect = 3.248e-44
Identity = 36/63 (57.14%), Postives = 46/63 (73.02%), Query Frame = 1

Query:  520 QVLVVANPANTNALILKEFAPSIPAKNITCLTRLNHNRALGQIF*KTEWPNIWMLEMVIIWGN 708
            +VLVV NPANTN LI+ ++APSIP +N T LTRL+HNRA+ Q+  K   PN   ++ V IWGN
Sbjct:  125 KVLVVGNPANTNCLIMSKYAPSIPKENFTALTRLDHNRAIYQVAAKAGVPNT-CVKNVCIWGN 186

[Close]

BLAST of EY684220 vs. ExPASy Swiss-Prot
Match: MDH_CHRVO (Malate dehydrogenase OS=Chromobacterium violaceum GN=mdh PE=3 SV=1)

HSP 1 Score: 133.65 bits (335), Expect = 3.521e-43
Identity = 70/121 (57.85%), Postives = 85/121 (70.25%), Query Frame = 3

Query:   72 VRVLVTGAAGQIGYALVPMIARGVMLGTDQPVILHMLDIPPAAEALNGVKMELVDAAFPLIKGVVATTDAVEACTGVNIAVMVGGFPRKEGMERKDVMSKNVSIYKAQASALEQHAAPNCK 434
            VRV VTGAAGQIGY+L+  IA G MLG DQPVILH+LD+P A  AL GV MEL D AFPL+ G+VAT D   A   V +A++VG  PR +GMERKD++  N +I+  Q  AL  HAA + K
Sbjct:    5 VRVAVTGAAGQIGYSLLFRIASGEMLGKDQPVILHLLDLPQAQTALKGVMMELEDCAFPLLAGMVATDDPNVAFKDVKVALLVGARPRSKGMERKDLLEANGAIFTVQGKALNDHAARDVK 125

HSP 2 Score: 63.5438 bits (153), Expect = 3.521e-43
Identity = 33/63 (52.38%), Postives = 42/63 (66.67%), Query Frame = 1

Query:  520 QVLVVANPANTNALILKEFAPSIPAKNITCLTRLNHNRALGQIF*KTEWPNIWMLEMVIIWGN 708
            +VLVV NPANTNA I  + AP +  KN T + RL+HNRAL QI  KT  P +  +E + +WGN
Sbjct:  125 KVLVVGNPANTNAWIAMKSAPDLDPKNFTAMLRLDHNRALSQIAAKTGKP-VASIEKLAVWGN 186

[Close]

BLAST of EY684220 vs. ExPASy Swiss-Prot
Match: MDH_RALEH (Malate dehydrogenase OS=Ralstonia eutropha (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) GN=mdh PE=3 SV=1)

HSP 1 Score: 128.257 bits (321), Expect = 2.246e-42
Identity = 67/126 (53.17%), Postives = 86/126 (68.25%), Query Frame = 3

Query:   57 MAPYTVRVLVTGAAGQIGYALVPMIARGVMLGTDQPVILHMLDIPPAAEALNGVKMELVDAAFPLIKGVVATTDAVEACTGVNIAVMVGGFPRKEGMERKDVMSKNVSIYKAQASALEQHAAPNCK 434
            MA   +RV VTGAAGQIGY+L+  IA G MLG DQPVIL +LD+P A +A+ GV MEL D AFPL+ GVV T D   A    ++A++VG  PR +GMERKD++  N  I+  Q  AL++ A+ N K
Sbjct:    1 MAKAPMRVAVTGAAGQIGYSLLFRIANGDMLGKDQPVILQLLDLPQAQQAVKGVVMELEDCAFPLLAGVVITDDPKVAFKDADVALLVGARPRSKGMERKDLLEANAQIFTVQGKALDEVASRNVK 126

HSP 2 Score: 66.2402 bits (160), Expect = 2.246e-42
Identity = 33/63 (52.38%), Postives = 44/63 (69.84%), Query Frame = 1

Query:  520 QVLVVANPANTNALILKEFAPSIPAKNITCLTRLNHNRALGQIF*KTEWPNIWMLEMVIIWGN 708
            +VLVV NPANTNA I  + AP++P +N T + RL+HNRAL QI  KT  P +  +E + +WGN
Sbjct:  126 KVLVVGNPANTNAYIAMKSAPNLPRENFTAMLRLDHNRALSQIAAKTGKP-VSSIEKLFVWGN 187

The following BLAST results are available for this feature:

BLAST of EY684220 vs. ExPASy Swiss-Prot

BLAST of EY684220 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 187


Match Name	E-value	Identity	Description
MDHC_MAIZE	3.636e-80	92.06	Malate dehydrogenase, cytoplasmic OS=Zea mays PE=2...	[more]
MDHC2_ARATH	4.155e-80	92.06	Malate dehydrogenase, cytoplasmic 2 OS=Arabidopsis...	[more]
MDHC1_ARATH	4.743e-80	90.48	Malate dehydrogenase, cytoplasmic 1 OS=Arabidopsis...	[more]
MDHC_MESCR	2.335e-79	91.27	Malate dehydrogenase, cytoplasmic OS=Mesembryanthe...	[more]
MDHC_ORYSJ	3.973e-79	88.89	Malate dehydrogenase, cytoplasmic OS=Oryza sativa ...	[more]
MDHC_BETVU	1.638e-77	88.89	Malate dehydrogenase, cytoplasmic OS=Beta vulgaris...	[more]
MDHC_MEDSA	2.787e-77	91.27	Malate dehydrogenase, cytoplasmic OS=Medicago sati...	[more]
MDHC_ECHGR	3.248e-44	51.61	Malate dehydrogenase, cytoplasmic OS=Echinococcus ...	[more]
MDH_CHRVO	3.521e-43	57.85	Malate dehydrogenase OS=Chromobacterium violaceum ...	[more]
MDH_RALEH	2.246e-42	53.17	Malate dehydrogenase OS=Ralstonia eutropha (strain...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C1-650-054-E03-CT.F Sweet orange leaf, young greenhouse plant Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY684220 ID=EY684220; Name=EY684220; organism=Citrus sinensis; type=EST; length=988bp
CCCACGCGTCCGGAAAAATCTTCTCTTATAAGTGTGATAATCTCTGATNN
GAATTAATGGCCCCTTATACAGTTCGCGTTCTTGTCACCGGCGCCGCAGG
GCAAATTGGATATGCACTTGTCCCCATGATTGCTAGGGGAGTGATGTTGG
GTACTGACCAGCCTGTGATTCTGCACATGCTTGATATACCACCTGCAGCA
GAGGCATTGAATGGTGTGAAGATGGAGTTGGTTGATGCTGCATTCCCTCT
TATTAAAGGTGTTGTTGCTACAACTGATGCTGTTGAGGCATGCACTGGGG
TCAATATTGCCGTTATGGTTGGTGGCTTTCCAAGAAAAGAAGGAATGGAG
AGAAAAGATGTGATGTCAAAGAACGTTTCAATCTACAAGGCTCAGGCTTC
TGCCCTGGAGCAGCACGCAGCTCCGAACTGCAAGGACCATGGAGATTCCT
TGTTGTAACTATATTGATGAAGCTCCTGAACTGTTGGTGCCATTACTGAA
TGGACCCTCTTGTTTATTTCAGGTTCTAGTTGTCGCTAACCCTGCAAACA
CCAATGCATTGATTCTGAAAGAATTTGCGCCCTCAATCCCTGCTAAAAAC
ATTACATGCTTGACCAGATTGAATCATAACAGGGCATTGGGACAAATATT
CTGAAAAACTGAATGGCCCAATATCTGGATGTTAGAAATGGTGATAATTT
GGGGAAATTCTTTATTCCCCCCGTTACCCCTGATGCCAACCTGGCAACTG
TCAAAACTGCCACTTGGGGAAAAAGCCCGGGCCGGTGAACCTTGGCAAGG
AAGAAAGGCGGGGGTTAAATGGAAAAATTTCCAAACCCCAGGCCCCAAAA
AGCGGGGGGGGGTTGCAAAACCTACAAAACACCCAAAAAAAACCCCCCTA
GAGGGGGGCCTCGGTGCGCGGCCGGGTACGAACCCCCGGGGCTGTTTGGG
AACCCAATAAATCTCGCGGGGGAAGAGTGGGGGGGGTT