EY679897

Overview
NameEY679897
Unique NameEY679897
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length923
Libraries
Library NameType
Sweet orange leaf, young greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY679897 vs. ExPASy Swiss-Prot
Match: AAKG1_RAT (5'-AMP-activated protein kinase subunit gamma-1 OS=Rattus norvegicus GN=Prkag1 PE=1 SV=3)

HSP 1 Score: 73.9442 bits (180), Expect = 1.405e-12
Identity = 47/126 (37.30%), Postives = 64/126 (50.79%), Query Frame = 3 
Query:   27 KVVALDIDLPVKQAFHILYEQGISMAPLWDFSKARFVGVLSASDFILILRELGNHGSNLTE-EELETHTISAWKEGKAYLNRQIDSHGKAFPRPLVYAGPNDNLKDVARKILHNEVATVPIIHSSS 401
            K+V  D  L VK+AF  L   G+  APLWD  K  FVG+L+ +DFI IL     + S L +  ELE H I  W+E   YL            +PLV   PN +L D    ++ N++  +P+I   S
Sbjct:   46 KLVVFDTSLQVKKAFFALVTNGVRAAPLWDSKKQSFVGMLTITDFINILHRY--YKSALVQIYELEEHKIETWRE--VYLQDSF--------KPLVCISPNASLFDAVSSLIRNKIHRLPVIDPES 159
[Close]
BLAST of EY679897 vs. ExPASy Swiss-Prot
Match: AAKG1_PIG (5'-AMP-activated protein kinase subunit gamma-1 OS=Sus scrofa GN=PRKAG1 PE=1 SV=2)

HSP 1 Score: 73.9442 bits (180), Expect = 1.405e-12
Identity = 47/126 (37.30%), Postives = 64/126 (50.79%), Query Frame = 3 
Query:   27 KVVALDIDLPVKQAFHILYEQGISMAPLWDFSKARFVGVLSASDFILILRELGNHGSNLTE-EELETHTISAWKEGKAYLNRQIDSHGKAFPRPLVYAGPNDNLKDVARKILHNEVATVPIIHSSS 401
            K+V  D  L VK+AF  L   G+  APLWD  K  FVG+L+ +DFI IL     + S L +  ELE H I  W+E   YL            +PLV   PN +L D    ++ N++  +P+I   S
Sbjct:   47 KLVVFDTSLQVKKAFFALVTNGVRAAPLWDSKKQSFVGMLTITDFINILHRY--YKSALVQIYELEEHKIETWRE--VYLQDSF--------KPLVCISPNASLFDAVSSLIRNKIHRLPVIDPES 160
[Close]
BLAST of EY679897 vs. ExPASy Swiss-Prot
Match: AAKG1_MOUSE (5'-AMP-activated protein kinase subunit gamma-1 OS=Mus musculus GN=Prkag1 PE=2 SV=2)

HSP 1 Score: 73.9442 bits (180), Expect = 1.405e-12
Identity = 47/126 (37.30%), Postives = 64/126 (50.79%), Query Frame = 3 
Query:   27 KVVALDIDLPVKQAFHILYEQGISMAPLWDFSKARFVGVLSASDFILILRELGNHGSNLTE-EELETHTISAWKEGKAYLNRQIDSHGKAFPRPLVYAGPNDNLKDVARKILHNEVATVPIIHSSS 401
            K+V  D  L VK+AF  L   G+  APLWD  K  FVG+L+ +DFI IL     + S L +  ELE H I  W+E   YL            +PLV   PN +L D    ++ N++  +P+I   S
Sbjct:   46 KLVVFDTSLQVKKAFFALVTNGVRAAPLWDSKKQSFVGMLTITDFINILHRY--YKSALVQIYELEEHKIETWRE--VYLQDSF--------KPLVCISPNASLFDAVSSLIRNKIHRLPVIDPES 159
[Close]
BLAST of EY679897 vs. ExPASy Swiss-Prot
Match: AAKG1_HUMAN (5'-AMP-activated protein kinase subunit gamma-1 OS=Homo sapiens GN=PRKAG1 PE=1 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 1.405e-12
Identity = 47/126 (37.30%), Postives = 64/126 (50.79%), Query Frame = 3 
Query:   27 KVVALDIDLPVKQAFHILYEQGISMAPLWDFSKARFVGVLSASDFILILRELGNHGSNLTE-EELETHTISAWKEGKAYLNRQIDSHGKAFPRPLVYAGPNDNLKDVARKILHNEVATVPIIHSSS 401
            K+V  D  L VK+AF  L   G+  APLWD  K  FVG+L+ +DFI IL     + S L +  ELE H I  W+E   YL            +PLV   PN +L D    ++ N++  +P+I   S
Sbjct:   47 KLVVFDTSLQVKKAFFALVTNGVRAAPLWDSKKQSFVGMLTITDFINILHRY--YKSALVQIYELEEHKIETWRE--VYLQDSF--------KPLVCISPNASLFDAVSSLIRNKIHRLPVIDPES 160
[Close]
BLAST of EY679897 vs. ExPASy Swiss-Prot
Match: AAKG1_BOVIN (5'-AMP-activated protein kinase subunit gamma-1 OS=Bos taurus GN=PRKAG1 PE=2 SV=2)

HSP 1 Score: 73.9442 bits (180), Expect = 1.405e-12
Identity = 47/126 (37.30%), Postives = 64/126 (50.79%), Query Frame = 3 
Query:   27 KVVALDIDLPVKQAFHILYEQGISMAPLWDFSKARFVGVLSASDFILILRELGNHGSNLTE-EELETHTISAWKEGKAYLNRQIDSHGKAFPRPLVYAGPNDNLKDVARKILHNEVATVPIIHSSS 401
            K+V  D  L VK+AF  L   G+  APLWD  K  FVG+L+ +DFI IL     + S L +  ELE H I  W+E   YL            +PLV   PN +L D    ++ N++  +P+I   S
Sbjct:   47 KLVVFDTSLQVKKAFFALVTNGVRAAPLWDSKKQSFVGMLTITDFINILHRY--YKSALVQIYELEEHKIETWRE--VYLQDSF--------KPLVCISPNASLFDAVSSLIRNKIHRLPVIDPES 160
[Close]
BLAST of EY679897 vs. ExPASy Swiss-Prot
Match: AAKG3_HUMAN (5'-AMP-activated protein kinase subunit gamma-3 OS=Homo sapiens GN=PRKAG3 PE=2 SV=3)

HSP 1 Score: 73.559 bits (179), Expect = 1.835e-12
Identity = 56/166 (33.73%), Postives = 83/166 (50.00%), Query Frame = 3 
Query:    3 DAWADAWGKVVALDIDLPVKQAFHILYEQGISMAPLWDFSKARFVGVLSASDFILILRELGNHGSNLTE-EELETHTISAWKEGKAYLNRQIDSHGKAFPRPLVYAGPNDNLKDVARKILHNEVATVPIIHSSSQDGSFPQLLHIASLSGILKCVCRYFRHCSSSL 497
            DA A +  K+V  D  L +K+AF  L   G+  APLWD  K  FVG+L+ +DFIL+L     + S L +  E+E H I  W+E   YL        +   +PLV   PND+L +    ++ N +  +P++   S +     +LHI +   +LK     F H   SL
Sbjct:  195 DAMATS-SKLVIFDTMLEIKKAFFALVANGVRAAPLWDSKKQSFVGMLTITDFILVLHRY--YRSPLVQIYEIEQHKIETWRE--IYL--------QGCFKPLVSISPNDSLFEAVYTLIKNRIHRLPVLDPVSGN-----VLHILTHKRLLK-----FLHIFGSL 337
[Close]
BLAST of EY679897 vs. ExPASy Swiss-Prot
Match: PRKAG_DICDI (5'-AMP-activated protein kinase subunit gamma OS=Dictyostelium discoideum GN=prkag PE=3 SV=3)

HSP 1 Score: 72.4034 bits (176), Expect = 4.088e-12
Identity = 54/175 (30.86%), Postives = 83/175 (47.43%), Query Frame = 3 
Query:   24 GKVVALDIDLPVKQAFHILYEQGISMAPLWDFSKARFVGVLSASDFI-LILRELGNHGSNLTEEELETHTISA-WKEGKAYLNRQIDSHGKAFPRPLVYAGPNDNLKDVARKILHNEVATVPIIHSSSQDGSFPQLLHIASLSGILKCVCRYFRHCSSSLPILKLPICAIPVGTW 542
            GKVV LD  L VK AF+ L E GI  APLW+  +  F G+++ SDFI ++L       SN   +++  H I   W+E                P  L+   P  NL D A  +L  ++  +P++     +     +LHI + S IL  + + F      L  L +PI ++ +GT+
Sbjct:  284 GKVVVLDTKLAVKSAFYALEENGIKSAPLWNSEQHDFTGMITVSDFIDILLYYYKKPKSNNIFQDMGIHRIETFWREISV-----------ERPSSLISTEPETNLYDAASLLLCYKIHRLPVVDKKDTN----SILHILTHSRILAFMMKSFPQLPEKL--LSIPIGSLGIGTF 441
[Close]
BLAST of EY679897 vs. ExPASy Swiss-Prot
Match: AAKG3_PIG (5'-AMP-activated protein kinase subunit gamma-3 OS=Sus scrofa GN=PRKAG3 PE=1 SV=2)

HSP 1 Score: 72.4034 bits (176), Expect = 4.088e-12
Identity = 52/153 (33.99%), Postives = 78/153 (50.98%), Query Frame = 3 
Query:    3 DAWADAWGKVVALDIDLPVKQAFHILYEQGISMAPLWDFSKARFVGVLSASDFILILRELGNHGSNLTE-EELETHTISAWKEGKAYLNRQIDSHGKAFPRPLVYAGPNDNLKDVARKILHNEVATVPIIHSSSQDGSFPQLLHIASLSGILK 458
            DA A +  K+V  D  L +K+AF  L   G+  APLWD  K  FVG+L+ +DFIL+L     + S L +  E+E H I  W+E   YL        +   +PLV   PND+L +    ++ N +  +P++     D     +LHI +   +LK
Sbjct:  220 DAMATS-SKLVIFDTMLEIKKAFFALVANGVRAAPLWDSKKQSFVGMLTITDFILVLHRY--YRSPLVQIYEIEEHKIETWRE--IYL--------QGCFKPLVSISPNDSLFEAVYALIKNRIHRLPVL-----DPVSGAVLHILTHKRLLK 354
[Close]
BLAST of EY679897 vs. ExPASy Swiss-Prot
Match: AAKG3_MOUSE (5'-AMP-activated protein kinase subunit gamma-3 OS=Mus musculus GN=Prkag3 PE=2 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 1.189e-11
Identity = 45/130 (34.62%), Postives = 69/130 (53.08%), Query Frame = 3 
Query:    3 DAWADAWGKVVALDIDLPVKQAFHILYEQGISMAPLWDFSKARFVGVLSASDFILILRELGNHGSNLTE-EELETHTISAWKEGKAYLNRQIDSHGKAFPRPLVYAGPNDNLKDVARKILHNEVATVPII 389
            DA A +  K+V  D  L +K+AF  +   G+  APLWD  K  FVG+L+ +DFIL+L     + S L +  E+E H I  W+E   YL        +   +PLV   PND+L +    ++ N +  +P++
Sbjct:  195 DAMATS-SKLVIFDTTLEIKKAFFAMVANGVRAAPLWDSKKQSFVGMLTITDFILVLHRY--YRSPLVQIYEIEEHKIETWRE--IYL--------QGCFKPLVSISPNDSLFEAVYALIKNRIHRLPVL 311
[Close]
BLAST of EY679897 vs. ExPASy Swiss-Prot
Match: AAKG3_BOVIN (5'-AMP-activated protein kinase subunit gamma-3 OS=Bos taurus GN=PRKAG3 PE=2 SV=2)

HSP 1 Score: 70.4774 bits (171), Expect = 1.553e-11
Identity = 51/153 (33.33%), Postives = 78/153 (50.98%), Query Frame = 3 
Query:    3 DAWADAWGKVVALDIDLPVKQAFHILYEQGISMAPLWDFSKARFVGVLSASDFILILRELGNHGSNLTE-EELETHTISAWKEGKAYLNRQIDSHGKAFPRPLVYAGPNDNLKDVARKILHNEVATVPIIHSSSQDGSFPQLLHIASLSGILK 458
            DA A +  K+V  D  L +K+AF  L   G+  APLWD  K  FVG+L+ +DFIL+L     + S L +  E+E H I  W+E   YL        +   +PLV   P+D+L +    ++ N +  +P++     D     +LHI +   +LK
Sbjct:  202 DAMATS-SKLVIFDTMLQIKKAFFALVANGVRAAPLWDSKKQSFVGMLTITDFILVLHRY--YRSPLVQIYEIEEHKIETWRE--IYL--------QGCFKPLVSISPSDSLFEAVYTLIKNRIHRLPVL-----DPVSGAVLHILTHKRLLK 336
The following BLAST results are available for this feature:
BLAST of EY679897 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 13
Match NameE-valueIdentityDescription
AAKG1_RAT1.405e-1237.305'-AMP-activated protein kinase subunit gamma-1 OS... [more]
AAKG1_PIG1.405e-1237.305'-AMP-activated protein kinase subunit gamma-1 OS... [more]
AAKG1_MOUSE1.405e-1237.305'-AMP-activated protein kinase subunit gamma-1 OS... [more]
AAKG1_HUMAN1.405e-1237.305'-AMP-activated protein kinase subunit gamma-1 OS... [more]
AAKG1_BOVIN1.405e-1237.305'-AMP-activated protein kinase subunit gamma-1 OS... [more]
AAKG3_HUMAN1.835e-1233.735'-AMP-activated protein kinase subunit gamma-3 OS... [more]
PRKAG_DICDI4.088e-1230.865'-AMP-activated protein kinase subunit gamma OS=D... [more]
AAKG3_PIG4.088e-1233.995'-AMP-activated protein kinase subunit gamma-3 OS... [more]
AAKG3_MOUSE1.189e-1134.625'-AMP-activated protein kinase subunit gamma-3 OS... [more]
AAKG3_BOVIN1.553e-1133.335'-AMP-activated protein kinase subunit gamma-3 OS... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-650-005-C02-CT.F Sweet orange leaf, young greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY679897 ID=EY679897; Name=EY679897; organism=Citrus sinensis; type=EST; length=923bp
CGGACGCGTGGGCGGACGCGTGGGGCAAGGTTGTAGCATTGGACATTGAT
CTACCTGTAAAACAAGCATTTCATATACTTTATGAGCAGGGTATCTCCAT
GGCTCCTCTTTGGGACTTCAGCAAGGCGCGGTTTGTTGGAGTTCTTAGTG
CGTCAGATTTTATATTGATTTTAAGAGAGCTTGGAAATCATGGCTCAAAT
CTCACAGAGGAAGAGCTTGAAACACACACCATATCTGCCTGGAAAGAGGG
GAAAGCGTATTTGAACAGACAAATTGATAGTCATGGGAAAGCATTTCCAA
GACCTCTTGTTTATGCGGGGCCGAATGATAACTTGAAGGATGTAGCGCGA
AAGATTTTGCATAATGAGGTGGCTACAGTTCCCATCATCCATTCATCTTC
ACAGGACGGTTCATTTCCTCAGCTACTACATATTGCATCCCTTTCTGGAA
TTCTAAAATGTGTTTGCAGGTATTTTAGGCATTGTTCTAGCTCGTTGCCC
ATTCTAAAGTTACCAATTTGTGCAATTCCTGTGGGTACTTGGGTTCCAAA
GATTGGAGAGCCTAATCGACGACCATTGGCAATGTTGAGACCTAGTGCTT
CTCTTAGTGCTGCATTAATTTTGTTTAGTCCCAGCTCAAGTAATTTCGAT
ACCCATAGTTGATGACAACAACTCGTTACTTTGAATCTACTGGCCGGAGT
GAAATTACCGGCTTTGGGAAAAGGACAAAGCTTATGCACACATTTAATCC
CAGTGGAAATGCCCTTTTCCTCGGGCATTTGCATTGGGGAAAAAATTCCT
ATTTCCCCTTTAGAAATTTGAAAAATTCAAAAAAGGCAAAAAAGTGGTTT
GCCGGCAAAAAACTTTTGGAAAAAAAGGGAAGGGAGGCGGTTGGGGAAAA
CCCCCGGGGGGTTTCCAGCGGGT
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