EY679865 | Citrus Genome Database

Overview


Name	EY679865
Unique Name	EY679865
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	916

Libraries


Library Name	Type
Sweet orange leaf, young greenhouse plant	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY679865 vs. ExPASy Swiss-Prot
Match: ARP_ARATH (Apurinic endonuclease-redox protein OS=Arabidopsis thaliana GN=ARP PE=2 SV=2)

HSP 1 Score: 104.375 bits (259), Expect = 8.479e-25
Identity = 68/178 (38.20%), Postives = 89/178 (50.00%), Query Frame = 1

Query:   79 LIG*IKPLSVTYGLGISPHDSEGRLVTAEFDSFFLLSCYFPNSGDGLRRL-----VMSSSILYYVANLFYSSKSFLTDVYAFNSSHTELQNGIHLLAAM*KSWKRRSLSF*VGILIVLIRR*TFITXXXXXXXXASQMKKDNLLGQTSYLKVFVNTFRAKIPGVEGYTYWGYRHGGRK 597
            +I  IKPLSV YG G+S HD+EGR+VTAEFDSF+L++ Y PNSGDGL+RL         ++  ++  L  S    LT     N +H E+            +  +RS  F                           +++    G     K FV+TFR + PGV GYTYWGYRHGGRK
Sbjct:  351 IISRIKPLSVRYGTGLSGHDTEGRIVTAEFDSFYLINTYVPNSGDGLKRLSYRIEEWDRTLSNHIKELEKSKPVVLTG--DLNCAHEEID-------IFNPAGNKRSAGF--------------------------TIEERQSFGANLLDKGFVDTFRKQHPGVVGYTYWGYRHGGRK 493

HSP 2 Score: 31.187 bits (69), Expect = 8.479e-25
Identity = 14/38 (36.84%), Postives = 22/38 (57.89%), Query Frame = 2

Query:  596 KTNRRGGIGYFLVTESLVENFHES*VCTGDTGKGNFPI 709
            KTN+   + YFLV++S+  N H+S +     G  + PI
Sbjct:  493 KTNKGWRLDYFLVSQSIAANVHDSYILPDINGSDHCPI 530

[Close]

BLAST of EY679865 vs. ExPASy Swiss-Prot
Match: APEA_DICDI (DNA-(apurinic or apyrimidinic site) lyase OS=Dictyostelium discoideum GN=apeA PE=2 SV=2)

HSP 1 Score: 64.6994 bits (156), Expect = 6.584e-21
Identity = 33/67 (49.25%), Postives = 39/67 (58.21%), Query Frame = 3

Query:  321 YRITEWDPSLSSYXXXXXXXXXXXXSGDLNCAHQEIDIYNPAGNRRIAGFTDEERQSFGTNFLSKGF 521
            YRI EWD    +Y             GDLN AH EID+ NP  N++ AGFT EER SF +NFL KG+
Sbjct:  225 YRIKEWDVDFQAYLEKLNATKPIIWCGDLNVAHTEIDLKNPKTNKKSAGFTIEERTSF-SNFLEKGY 290

HSP 2 Score: 57.7658 bits (138), Expect = 6.584e-21
Identity = 23/46 (50.00%), Postives = 38/46 (82.61%), Query Frame = 1

Query:   94 KPLSVTYGLGISPHDSEGRLVTAEFDSFFLLSCYFPNSGD-GLRRL 228
            KP ++T+G+GI+ HD+EGR++T E+D F++++ Y PN+G  GL+RL
Sbjct:  178 KPNAITFGIGIAKHDNEGRVITLEYDQFYIVNTYIPNAGTRGLQRL 223

[Close]

BLAST of EY679865 vs. ExPASy Swiss-Prot
Match: APEX1_PANTR (DNA-(apurinic or apyrimidinic site) lyase OS=Pan troglodytes GN=APEX1 PE=3 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 1.647e-18
Identity = 26/44 (59.09%), Postives = 33/44 (75.00%), Query Frame = 1

Query:   97 PLSVTYGLGISPHDSEGRLVTAEFDSFFLLSCYFPNSGDGLRRL 228
            PL V+YG+G   HD EGR++ AEFDSF L++ Y PN+G GL RL
Sbjct:  139 PLKVSYGIGEEEHDQEGRVIVAEFDSFVLVTAYVPNAGRGLVRL 182

HSP 2 Score: 54.6842 bits (130), Expect = 1.647e-18
Identity = 24/54 (44.44%), Postives = 31/54 (57.41%), Query Frame = 3

Query:  336 WDPSLSSYXXXXXXXXXXXXSGDLNCAHQEIDIYNPAGNRRIAGFTDEERQSFG 497
            WD +   +             GDLN AH+EID+ NP GN++ AGFT +ERQ FG
Sbjct:  188 WDEAFRKFLKGLASRKPLVLCGDLNVAHEEIDLRNPKGNKKNAGFTPQERQGFG 241

[Close]

BLAST of EY679865 vs. ExPASy Swiss-Prot
Match: APEX1_PANPA (DNA-(apurinic or apyrimidinic site) lyase OS=Pan paniscus GN=APEX1 PE=3 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 1.647e-18
Identity = 26/44 (59.09%), Postives = 33/44 (75.00%), Query Frame = 1

Query:   97 PLSVTYGLGISPHDSEGRLVTAEFDSFFLLSCYFPNSGDGLRRL 228
            PL V+YG+G   HD EGR++ AEFDSF L++ Y PN+G GL RL
Sbjct:  139 PLKVSYGIGEEEHDQEGRVIVAEFDSFVLVTAYVPNAGRGLVRL 182

HSP 2 Score: 54.6842 bits (130), Expect = 1.647e-18
Identity = 24/54 (44.44%), Postives = 31/54 (57.41%), Query Frame = 3

Query:  336 WDPSLSSYXXXXXXXXXXXXSGDLNCAHQEIDIYNPAGNRRIAGFTDEERQSFG 497
            WD +   +             GDLN AH+EID+ NP GN++ AGFT +ERQ FG
Sbjct:  188 WDEAFRKFLKGLASRKPLVLCGDLNVAHEEIDLRNPKGNKKNAGFTPQERQGFG 241

[Close]

BLAST of EY679865 vs. ExPASy Swiss-Prot
Match: APEX1_HUMAN (DNA-(apurinic or apyrimidinic site) lyase OS=Homo sapiens GN=APEX1 PE=1 SV=2)

HSP 1 Score: 59.6918 bits (143), Expect = 1.647e-18
Identity = 26/44 (59.09%), Postives = 33/44 (75.00%), Query Frame = 1

Query:   97 PLSVTYGLGISPHDSEGRLVTAEFDSFFLLSCYFPNSGDGLRRL 228
            PL V+YG+G   HD EGR++ AEFDSF L++ Y PN+G GL RL
Sbjct:  139 PLKVSYGIGDEEHDQEGRVIVAEFDSFVLVTAYVPNAGRGLVRL 182

HSP 2 Score: 54.6842 bits (130), Expect = 1.647e-18
Identity = 24/54 (44.44%), Postives = 31/54 (57.41%), Query Frame = 3

Query:  336 WDPSLSSYXXXXXXXXXXXXSGDLNCAHQEIDIYNPAGNRRIAGFTDEERQSFG 497
            WD +   +             GDLN AH+EID+ NP GN++ AGFT +ERQ FG
Sbjct:  188 WDEAFRKFLKGLASRKPLVLCGDLNVAHEEIDLRNPKGNKKNAGFTPQERQGFG 241

[Close]

BLAST of EY679865 vs. ExPASy Swiss-Prot
Match: APEX1_PONPY (DNA-(apurinic or apyrimidinic site) lyase OS=Pongo pygmaeus GN=APEX1 PE=3 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 2.142e-18
Identity = 26/44 (59.09%), Postives = 33/44 (75.00%), Query Frame = 1

Query:   97 PLSVTYGLGISPHDSEGRLVTAEFDSFFLLSCYFPNSGDGLRRL 228
            PL V+YG+G   HD EGR++ AEFDSF L++ Y PN+G GL RL
Sbjct:  139 PLKVSYGIGEEEHDQEGRVIVAEFDSFVLVTAYVPNAGRGLVRL 182

HSP 2 Score: 54.299 bits (129), Expect = 2.142e-18
Identity = 24/54 (44.44%), Postives = 31/54 (57.41%), Query Frame = 3

Query:  336 WDPSLSSYXXXXXXXXXXXXSGDLNCAHQEIDIYNPAGNRRIAGFTDEERQSFG 497
            WD +   +             GDLN AH+EID+ NP GN++ AGFT +ERQ FG
Sbjct:  188 WDEAFRRFLKGLASRKPLVLCGDLNVAHEEIDLRNPKGNKKNAGFTPQERQGFG 241

[Close]

BLAST of EY679865 vs. ExPASy Swiss-Prot
Match: APEX1_GORGO (DNA-(apurinic or apyrimidinic site) lyase OS=Gorilla gorilla gorilla GN=APEX1 PE=3 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 2.142e-18
Identity = 26/44 (59.09%), Postives = 33/44 (75.00%), Query Frame = 1

Query:   97 PLSVTYGLGISPHDSEGRLVTAEFDSFFLLSCYFPNSGDGLRRL 228
            PL V+YG+G   HD EGR++ AEFDSF L++ Y PN+G GL RL
Sbjct:  139 PLKVSYGIGEEEHDQEGRVIVAEFDSFVLVTAYVPNAGRGLVRL 182

HSP 2 Score: 54.299 bits (129), Expect = 2.142e-18
Identity = 24/54 (44.44%), Postives = 31/54 (57.41%), Query Frame = 3

Query:  336 WDPSLSSYXXXXXXXXXXXXSGDLNCAHQEIDIYNPAGNRRIAGFTDEERQSFG 497
            WD +   +             GDLN AH+EID+ NP GN++ AGFT +ERQ FG
Sbjct:  188 WDEAFRRFLKGLASRKPLVLCGDLNVAHEEIDLRNPKGNKKNAGFTPQERQGFG 241

[Close]

BLAST of EY679865 vs. ExPASy Swiss-Prot
Match: APEX1_RAT (DNA-(apurinic or apyrimidinic site) lyase OS=Rattus norvegicus GN=Apex1 PE=2 SV=2)

HSP 1 Score: 58.5362 bits (140), Expect = 3.622e-18
Identity = 25/44 (56.82%), Postives = 33/44 (75.00%), Query Frame = 1

Query:   97 PLSVTYGLGISPHDSEGRLVTAEFDSFFLLSCYFPNSGDGLRRL 228
            PL V+YG+G   HD EGR++ AEF+SF L++ Y PN+G GL RL
Sbjct:  138 PLKVSYGIGEEEHDQEGRVIVAEFESFILVTAYVPNAGRGLVRL 181

HSP 2 Score: 54.6842 bits (130), Expect = 3.622e-18
Identity = 24/54 (44.44%), Postives = 31/54 (57.41%), Query Frame = 3

Query:  336 WDPSLSSYXXXXXXXXXXXXSGDLNCAHQEIDIYNPAGNRRIAGFTDEERQSFG 497
            WD +   +             GDLN AH+EID+ NP GN++ AGFT +ERQ FG
Sbjct:  187 WDEAFRKFLKDLASRKPLVLCGDLNVAHEEIDLRNPKGNKKNAGFTPQERQGFG 240

[Close]

BLAST of EY679865 vs. ExPASy Swiss-Prot
Match: APEX1_MOUSE (DNA-(apurinic or apyrimidinic site) lyase OS=Mus musculus GN=Apex1 PE=1 SV=2)

HSP 1 Score: 58.151 bits (139), Expect = 4.710e-18
Identity = 25/44 (56.82%), Postives = 33/44 (75.00%), Query Frame = 1

Query:   97 PLSVTYGLGISPHDSEGRLVTAEFDSFFLLSCYFPNSGDGLRRL 228
            PL V+YG+G   HD EGR++ AEF+SF L++ Y PN+G GL RL
Sbjct:  138 PLKVSYGIGEEEHDQEGRVIVAEFESFVLVTAYVPNAGRGLVRL 181

HSP 2 Score: 54.6842 bits (130), Expect = 4.710e-18
Identity = 24/54 (44.44%), Postives = 31/54 (57.41%), Query Frame = 3

Query:  336 WDPSLSSYXXXXXXXXXXXXSGDLNCAHQEIDIYNPAGNRRIAGFTDEERQSFG 497
            WD +   +             GDLN AH+EID+ NP GN++ AGFT +ERQ FG
Sbjct:  187 WDEAFRKFLKDLASRKPLVLCGDLNVAHEEIDLRNPKGNKKNAGFTPQERQGFG 240

[Close]

BLAST of EY679865 vs. ExPASy Swiss-Prot
Match: APEX1_BOVIN (DNA-(apurinic or apyrimidinic site) lyase OS=Bos taurus GN=APEX1 PE=1 SV=2)

HSP 1 Score: 57.3806 bits (137), Expect = 7.961e-18
Identity = 24/44 (54.55%), Postives = 33/44 (75.00%), Query Frame = 1

Query:   97 PLSVTYGLGISPHDSEGRLVTAEFDSFFLLSCYFPNSGDGLRRL 228
            PL V+YG+G   HD EGR++ AE+D+F L++ Y PN+G GL RL
Sbjct:  139 PLKVSYGIGEEEHDQEGRVIVAEYDAFVLVTAYVPNAGRGLVRL 182

HSP 2 Score: 54.6842 bits (130), Expect = 7.961e-18
Identity = 24/54 (44.44%), Postives = 31/54 (57.41%), Query Frame = 3

Query:  336 WDPSLSSYXXXXXXXXXXXXSGDLNCAHQEIDIYNPAGNRRIAGFTDEERQSFG 497
            WD +   +             GDLN AH+EID+ NP GN++ AGFT +ERQ FG
Sbjct:  188 WDEAFRKFLKGLASRKPLVLCGDLNVAHEEIDLRNPKGNKKNAGFTPQERQGFG 241

The following BLAST results are available for this feature:

BLAST of EY679865 vs. ExPASy Swiss-Prot

BLAST of EY679865 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 11


Match Name	E-value	Identity	Description
ARP_ARATH	8.479e-25	38.20	Apurinic endonuclease-redox protein OS=Arabidopsis...	[more]
APEA_DICDI	6.584e-21	49.25	DNA-(apurinic or apyrimidinic site) lyase OS=Dicty...	[more]
APEX1_PANTR	1.647e-18	59.09	DNA-(apurinic or apyrimidinic site) lyase OS=Pan t...	[more]
APEX1_PANPA	1.647e-18	59.09	DNA-(apurinic or apyrimidinic site) lyase OS=Pan p...	[more]
APEX1_HUMAN	1.647e-18	59.09	DNA-(apurinic or apyrimidinic site) lyase OS=Homo ...	[more]
APEX1_PONPY	2.142e-18	59.09	DNA-(apurinic or apyrimidinic site) lyase OS=Pongo...	[more]
APEX1_GORGO	2.142e-18	59.09	DNA-(apurinic or apyrimidinic site) lyase OS=Goril...	[more]
APEX1_RAT	3.622e-18	56.82	DNA-(apurinic or apyrimidinic site) lyase OS=Rattu...	[more]
APEX1_MOUSE	4.710e-18	56.82	DNA-(apurinic or apyrimidinic site) lyase OS=Mus m...	[more]
APEX1_BOVIN	7.961e-18	54.55	DNA-(apurinic or apyrimidinic site) lyase OS=Bos t...	[more]

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Properties


Property Name	Value
Genbank description	CS00-C1-650-004-H03-CT.F Sweet orange leaf, young greenhouse plant Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY679865 ID=EY679865; Name=EY679865; organism=Citrus sinensis; type=EST; length=916bp
TTGATTTTTGCATTGCCACTATTTCTTTTACTTCAGTGATGATTTTGCTA
AATGGCCCCCGTTCTAAAGTTTTTAACACTTATTGGGTAGATAAAACCAC
TCTCAGTCACATATGGCCTAGGCATATCACCCCACGACAGTGAGGGACGG
CTGGTGACAGCAGAGTTTGATTCATTTTTTCTGTTAAGCTGTTATTTTCC
AAACTCTGGAGATGGCTTGAGAAGACTGGTGATGAGCTCAAGCATCTTGT
ATTATGTTGCTAATCTATTTTATTCTTCGAAGAGTTTTTTGACTGATGTC
TATGCTTTCAATTCTAGTCATACAGAATTACAGAATGGGATCCATCTCTT
AGCAGCTATGTGAAAGAGCTGGAAAAGAAGAAGCCTGTCGTTTTGAGTGG
GGATCTTAATTGTGCTCATCAGGAGATAGACATTTATAACCCTGCTGGAA
ATAGAAGAATTGCTGGCTTCACAGATGAAGAAAGACAATCTTTTGGGACA
AACTTCTTATCTAAAGGTTTTTGTAAATACCTTTCGAGCCAAAATTCCTG
GTGTTGAAGGCTATACTTATTGGGGGTATAGGCATGGGGGGCGCAAAAAC
CAATAGAAGGGGGGGGATTGGCTACTTCCTAGTAACCGAATCTCTAGTAG
AAAACTTTCATGAATCATAAGTATGTACGGGAGATACCGGAAAGGGAAAT
TTCCCAATTTGGAATTGATTCACAATTTAAAATATTCCGAGCAGAGATCA
TAACAGCTAGCAATCAGGCACACCAAGCAAAAGAATGGTTTGATATTTCC
GTGTGCAACAATTTTTTAAAAAAGGTGGAAATATTCCCCACCGAACTACG
AGCACATGTTCTCTTGTTTGTGCGAGGCCATTCTATGTGAAAAGCATTGA
ATTCTTTGTGTTAGTA