EY744781

Overview
NameEY744781
Unique NameEY744781
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length930
Libraries
Library NameType
Sweet orange flower, greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
EY744781_ssr827 microsatellite EY744781_ssr827:827..836. BLAST: Citrus ESTs to Prunus persica proteins V1
Homology
[Close]
BLAST of EY744781 vs. ExPASy Swiss-Prot
Match: EF2_BETVU (Elongation factor 2 OS=Beta vulgaris PE=2 SV=1)

HSP 1 Score: 198.364 bits (503), Expect = 4.991e-50
Identity = 95/109 (87.16%), Postives = 102/109 (93.58%), Query Frame = 3 
Query:   12 QAPEQALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPDTQAAQLVADIRKRKGLKEQMTPLSDFEDKL 338
            QAPE ALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPV+ESFGFS TLRA+TSGQAFPQCVFDHW+MM SDPLE  +QA+ LV+ IRKRKGLKEQMTPLS+FEDKL
Sbjct:  735 QAPENALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVVESFGFSSTLRASTSGQAFPQCVFDHWEMMPSDPLEAGSQASTLVSVIRKRKGLKEQMTPLSEFEDKL 843
[Close]
BLAST of EY744781 vs. ExPASy Swiss-Prot
Match: EF2_CHLKE (Elongation factor 2 OS=Chlorella kessleri PE=2 SV=1)

HSP 1 Score: 172.555 bits (436), Expect = 2.933e-42
Identity = 80/109 (73.39%), Postives = 94/109 (86.24%), Query Frame = 3 
Query:   12 QAPEQALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPDTQAAQLVADIRKRKGLKEQMTPLSDFEDKL 338
            Q PEQA+GG+YSVLNQKRG VFEE+QRPGTP++N+KAYLPVIESFGF+ TLRAAT+GQAFPQCVFDHW+ M SDP +  +QA  LV DIRKRKGLK +   LS++EDKL
Sbjct:  737 QCPEQAMGGVYSVLNQKRGMVFEELQRPGTPIFNLKAYLPVIESFGFTSTLRAATAGQAFPQCVFDHWEAMGSDPTQVGSQANTLVMDIRKRKGLKPEPAALSEYEDKL 845
[Close]
BLAST of EY744781 vs. ExPASy Swiss-Prot
Match: EF2_SCHPO (Elongation factor 2 OS=Schizosaccharomyces pombe GN=eft1 PE=1 SV=2)

HSP 1 Score: 153.295 bits (386), Expect = 1.841e-36
Identity = 72/109 (66.06%), Postives = 86/109 (78.90%), Query Frame = 3 
Query:   12 QAPEQALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPDTQAAQLVADIRKRKGLKEQMTPLSDFEDKL 338
            Q  E A+GGIYSVLN+KRGHVF E QR GTPLYNIKAYLPV ESFGF+G LR AT+GQAFPQ VFDHW  MS DPL+P ++  Q+V + RKRKGLKE +   +++ D+L
Sbjct:  734 QVSENAMGGIYSVLNKKRGHVFSEEQRVGTPLYNIKAYLPVNESFGFTGELRQATAGQAFPQLVFDHWSPMSGDPLDPTSKPGQIVCEARKRKGLKENVPDYTEYYDRL 842
[Close]
BLAST of EY744781 vs. ExPASy Swiss-Prot
Match: EF2_ASHGO (Elongation factor 2 OS=Ashbya gossypii GN=EFT1 PE=3 SV=1)

HSP 1 Score: 148.288 bits (373), Expect = 5.923e-35
Identity = 69/109 (63.30%), Postives = 84/109 (77.06%), Query Frame = 3 
Query:   12 QAPEQALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPDTQAAQLVADIRKRKGLKEQMTPLSDFEDKL 338
            Q PEQA+GGIYSVLN+KRG V  E QRPGTPL+ +KAYLPV ESFGF+G LR AT GQAFPQ VFDHW  + +DPL+P T+A ++V + RKR GLKE +    ++ DKL
Sbjct:  734 QCPEQAVGGIYSVLNRKRGQVVSEEQRPGTPLFTVKAYLPVNESFGFTGELRQATGGQAFPQMVFDHWATLGTDPLDPTTKAGEIVVEARKRHGLKENVPGWQEYYDKL 842
[Close]
BLAST of EY744781 vs. ExPASy Swiss-Prot
Match: EF2_KLULA (Elongation factor 2 OS=Kluyveromyces lactis GN=EFT1 PE=3 SV=1)

HSP 1 Score: 147.517 bits (371), Expect = 1.010e-34
Identity = 67/109 (61.47%), Postives = 85/109 (77.98%), Query Frame = 3 
Query:   12 QAPEQALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPDTQAAQLVADIRKRKGLKEQMTPLSDFEDKL 338
            Q PEQA+GGIYSVLN+KRG V  E QRPGTPL+ +KAYLP+ ESFGF+G LR AT GQAFPQ VFDHW  + +DPL+P T+A ++V   RKR+G+KE++    ++ DKL
Sbjct:  734 QCPEQAIGGIYSVLNKKRGQVVSEEQRPGTPLFTVKAYLPINESFGFTGELRQATGGQAFPQMVFDHWATLGTDPLDPSTKAGEIVLAARKRQGMKEEVPGWQEYYDKL 842
[Close]
BLAST of EY744781 vs. ExPASy Swiss-Prot
Match: EF2_YEAST (Elongation factor 2 OS=Saccharomyces cerevisiae GN=EFT1 PE=1 SV=1)

HSP 1 Score: 146.747 bits (369), Expect = 1.723e-34
Identity = 68/109 (62.39%), Postives = 84/109 (77.06%), Query Frame = 3 
Query:   12 QAPEQALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPDTQAAQLVADIRKRKGLKEQMTPLSDFEDKL 338
            Q PEQA+GGIYSVLN+KRG V  E QRPGTPL+ +KAYLPV ESFGF+G LR AT GQAFPQ VFDHW  + SDPL+P ++A ++V   RKR G+KE++    ++ DKL
Sbjct:  734 QCPEQAVGGIYSVLNKKRGQVVSEEQRPGTPLFTVKAYLPVNESFGFTGELRQATGGQAFPQMVFDHWSTLGSDPLDPTSKAGEIVLAARKRHGMKEEVPGWQEYYDKL 842
[Close]
BLAST of EY744781 vs. ExPASy Swiss-Prot
Match: EF2_SACKL (Elongation factor 2 OS=Saccharomyces kluyveri GN=EFT2 PE=3 SV=1)

HSP 1 Score: 146.747 bits (369), Expect = 1.723e-34
Identity = 68/109 (62.39%), Postives = 84/109 (77.06%), Query Frame = 3 
Query:   12 QAPEQALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPDTQAAQLVADIRKRKGLKEQMTPLSDFEDKL 338
            Q PEQA+GGIYSVLN+KRG V  E QRPGTPL+ +KAYLPV ESFGF+G LR AT GQAFPQ VFDHW  + +DPL+P T+A ++V   RKR G+KE++    ++ DKL
Sbjct:  734 QCPEQAVGGIYSVLNRKRGQVVSEEQRPGTPLFTVKAYLPVNESFGFTGELRQATGGQAFPQMVFDHWATLGTDPLDPTTKAGEIVTAARKRHGMKEEVPGWQEYYDKL 842
[Close]
BLAST of EY744781 vs. ExPASy Swiss-Prot
Match: EF2_CANGA (Elongation factor 2 OS=Candida glabrata GN=EFT1 PE=3 SV=1)

HSP 1 Score: 146.747 bits (369), Expect = 1.723e-34
Identity = 68/109 (62.39%), Postives = 85/109 (77.98%), Query Frame = 3 
Query:   12 QAPEQALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPDTQAAQLVADIRKRKGLKEQMTPLSDFEDKL 338
            Q PEQA+GGIYSVLN+KRG V  E QRPGTPL+ +KAYLPV ESFGF+G LR AT GQAFPQ VFDHW  ++SDPL+P ++A ++V   RKR G+KE++    ++ DKL
Sbjct:  734 QCPEQAVGGIYSVLNKKRGQVVSEEQRPGTPLFTVKAYLPVNESFGFTGELRQATGGQAFPQMVFDHWATLNSDPLDPTSKAGEIVTAARKRHGMKEEVPGWQEYYDKL 842
[Close]
BLAST of EY744781 vs. ExPASy Swiss-Prot
Match: EF2_CRIGR (Elongation factor 2 OS=Cricetulus griseus GN=EEF2 PE=3 SV=3)

HSP 1 Score: 145.591 bits (366), Expect = 3.839e-34
Identity = 67/109 (61.47%), Postives = 84/109 (77.06%), Query Frame = 3 
Query:   12 QAPEQALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPDTQAAQLVADIRKRKGLKEQMTPLSDFEDKL 338
            Q PEQ +GGIY VLN+KRGHVFEE Q  GTP++ +KAYLPV ESFGF+  LR+ T GQAFPQCVFDHW ++  DP +  ++ +Q+VA+ RKRKGLKE +  L +F DKL
Sbjct:  750 QCPEQVVGGIYGVLNRKRGHVFEESQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRGSQVVAETRKRKGLKEGIPALDNFLDKL 858
[Close]
BLAST of EY744781 vs. ExPASy Swiss-Prot
Match: EF2_RAT (Elongation factor 2 OS=Rattus norvegicus GN=Eef2 PE=1 SV=4)

HSP 1 Score: 145.206 bits (365), Expect = 5.014e-34
Identity = 67/109 (61.47%), Postives = 84/109 (77.06%), Query Frame = 3 
Query:   12 QAPEQALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPDTQAAQLVADIRKRKGLKEQMTPLSDFEDKL 338
            Q PEQ +GGIY VLN+KRGHVFEE Q  GTP++ +KAYLPV ESFGF+  LR+ T GQAFPQCVFDHW ++  DP +  ++ +Q+VA+ RKRKGLKE +  L +F DKL
Sbjct:  750 QCPEQVVGGIYGVLNRKRGHVFEESQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPALDNFLDKL 858
The following BLAST results are available for this feature:
BLAST of EY744781 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 40
Match NameE-valueIdentityDescription
EF2_BETVU4.991e-5087.16Elongation factor 2 OS=Beta vulgaris PE=2 SV=1[more]
EF2_CHLKE2.933e-4273.39Elongation factor 2 OS=Chlorella kessleri PE=2 SV=... [more]
EF2_SCHPO1.841e-3666.06Elongation factor 2 OS=Schizosaccharomyces pombe G... [more]
EF2_ASHGO5.923e-3563.30Elongation factor 2 OS=Ashbya gossypii GN=EFT1 PE=... [more]
EF2_KLULA1.010e-3461.47Elongation factor 2 OS=Kluyveromyces lactis GN=EFT... [more]
EF2_YEAST1.723e-3462.39Elongation factor 2 OS=Saccharomyces cerevisiae GN... [more]
EF2_SACKL1.723e-3462.39Elongation factor 2 OS=Saccharomyces kluyveri GN=E... [more]
EF2_CANGA1.723e-3462.39Elongation factor 2 OS=Candida glabrata GN=EFT1 PE... [more]
EF2_CRIGR3.839e-3461.47Elongation factor 2 OS=Cricetulus griseus GN=EEF2 ... [more]
EF2_RAT5.014e-3461.47Elongation factor 2 OS=Rattus norvegicus GN=Eef2 P... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C5-003-003-E01-CT.F Sweet orange flower, greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY744781 ID=EY744781; Name=EY744781; organism=Citrus sinensis; type=EST; length=930bp
ACCCNCGTCCGCAGGCTCCCGAGCAGGCCCTTGGTGGTATTTACAGTGTT
CTCAATCAGAAACGTGGTCACGTGTTCGAGGAAATGCAGAGACCTGGTAC
CCCCCTTTACAACATCAAGGCTTACCTTCCCGTCATCGAGTCCTTTGGAT
TCTCAGGCACTTTGAGGGCTGCAACGTCAGGACAGGCCTTCCCACAATGT
GTGTTTGATCACTGGGACATGATGTCATCCGATCCACTGGAGCCTGATAC
ACAGGCTGCACAGCTTGTTGCTGATATCAGGAAAAGGAAGGGCTTGAAGG
AGCAAATGACACCACTATCTGACTTTGAGGACAAGCTGTAAACATTTCGT
CTTGACATCAAGGGTGAGATTGCGCAGCTTGTTGCTGATATCACGAACAG
GGAGCAAATGACACCGCTATCCGAGTAAAGGACCAACTGTAAACATTTCA
TCTTGACATCAAGGGCTGAAATTGCCAGAAATATTTTTTGCTGATGCTTA
TTTTAGTTCCCAGATCTTTCGATATCCTCGTTGAGTTAAGAAAAAAATAA
TAATGGAGAAATGGGTTCAGTCAGCCACGGGCCGGCGCCCGACGGTACCA
CTATGAACAACCACGGATTCGCCGAGACGACACAAAAGAACGACACCCAC
ACCGAAAACACGCCGGCGCTGGAAAGTCATAAACAACCCCCACCCACCGA
ACAAGAATCCCAACACGACACACACCTCACNCCGAACAAACAGCCGACCC
ACGCACCGAACCACCGCACCCCCCCCCACCCGCAAGCAAGCGATCAGACG
CAGGCAGGGACAAGACGCCCACGAAGCACACACACAATGAAGAAACGCAA
GGGGGGACCGCCGCAAGAACGACCACCAAACAAGCGAAACCAGCACAAAC
ACAAGAAGCACCAAGACAAGCAAACACCCC
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