EY679511

Overview
NameEY679511
Unique NameEY679511
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length938
Libraries
Library NameType
Sweet orange leaf, infected with CiLVcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY679511 vs. ExPASy Swiss-Prot
Match: FADE_ECOLI (Acyl-coenzyme A dehydrogenase OS=Escherichia coli (strain K12) GN=fadE PE=2 SV=2)

HSP 1 Score: 155.221 bits (391), Expect = 1.529e-48
Identity = 72/128 (56.25%), Postives = 90/128 (70.31%), Query Frame = -1 
Query:   12 KDLGISLALIPTDTPGVEIGRRHLPLGAAFMNGPNSGKDVFIPLEFLIGGQEMLGKGWMMLMNCLSVGRSISLPAVGTGAAKFTSLVTGQYAQVREQFNVPLAAFESIQEALARIGGNVKRRDSARML 395
            +DLGI+ ALIPT TPGVEIGRRH PL   F NGP  GKDVF+P++++IGG +M G+GW ML+ CLSVGR I+LP+  TG  K  +L TG YA +R QF + +   E I+E LARI GN    D+A  L
Sbjct:  306 EDLGITCALIPTTTPGVEIGRRHFPLNVPFQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALATGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASL 433

HSP 2 Score: 55.8398 bits (133), Expect = 1.529e-48
Identity = 26/46 (56.52%), Postives = 31/46 (67.39%), Query Frame = -3 
Query:  394 GNGKARETLGWRLNWEKRYITLGPVATLLGLASKAHDPVHLLGDKE 531
            G  + ++ LG RL W KRYITL P+AT+LGLA K  DP  LLG  E
Sbjct:  261 GEWQGQQVLGMRLTWNKRYITLAPIATVLGLAFKLSDPEKLLGGAE 306

HSP 3 Score: 25.0238 bits (53), Expect = 1.529e-48
Identity = 10/19 (52.63%), Postives = 15/19 (78.95%), Query Frame = -2 
Query:  515 AGSIVLTPGVICKGEWEGK 571
            AG+I  T G++C GEW+G+
Sbjct:  249 AGAIPDT-GIVCMGEWQGQ 266
[Close]
BLAST of EY679511 vs. ExPASy Swiss-Prot
Match: FADE_ECO57 (Acyl-coenzyme A dehydrogenase OS=Escherichia coli O157:H7 GN=fadE PE=3 SV=2)

HSP 1 Score: 154.836 bits (390), Expect = 1.989e-48
Identity = 72/128 (56.25%), Postives = 90/128 (70.31%), Query Frame = -1 
Query:   12 KDLGISLALIPTDTPGVEIGRRHLPLGAAFMNGPNSGKDVFIPLEFLIGGQEMLGKGWMMLMNCLSVGRSISLPAVGTGAAKFTSLVTGQYAQVREQFNVPLAAFESIQEALARIGGNVKRRDSARML 395
            +DLGI+ ALIPT TPGVEIGRRH PL   F NGP  GKDVF+P++++IGG +M G+GW ML+ CLSVGR I+LP+  TG  K  +L TG YA +R QF + +   E I+E LARI GN    D+A  L
Sbjct:  306 EDLGITCALIPTTTPGVEIGRRHFPLNVPFQNGPTLGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALATGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASL 433

HSP 2 Score: 55.8398 bits (133), Expect = 1.989e-48
Identity = 26/46 (56.52%), Postives = 31/46 (67.39%), Query Frame = -3 
Query:  394 GNGKARETLGWRLNWEKRYITLGPVATLLGLASKAHDPVHLLGDKE 531
            G  + ++ LG RL W KRYITL P+AT+LGLA K  DP  LLG  E
Sbjct:  261 GEWQGQQVLGMRLTWNKRYITLAPIATVLGLAFKLSDPEKLLGGAE 306

HSP 3 Score: 25.0238 bits (53), Expect = 1.989e-48
Identity = 10/19 (52.63%), Postives = 15/19 (78.95%), Query Frame = -2 
Query:  515 AGSIVLTPGVICKGEWEGK 571
            AG+I  T G++C GEW+G+
Sbjct:  249 AGAIPDT-GIVCMGEWQGQ 266
[Close]
BLAST of EY679511 vs. ExPASy Swiss-Prot
Match: FADE_SALTY (Acyl-coenzyme A dehydrogenase OS=Salmonella typhimurium GN=fadE PE=2 SV=1)

HSP 1 Score: 152.14 bits (383), Expect = 5.697e-48
Identity = 69/129 (53.49%), Postives = 90/129 (69.77%), Query Frame = -1 
Query:   12 KKDLGISLALIPTDTPGVEIGRRHLPLGAAFMNGPNSGKDVFIPLEFLIGGQEMLGKGWMMLMNCLSVGRSISLPAVGTGAAKFTSLVTGQYAQVREQFNVPLAAFESIQEALARIGGNVKRRDSARML 398
            +++LGI+ ALIPT TPGVEIGRRH PL   F NGP  G D+F+P++++IGG +M G+GW ML+ CLSVGR I+LP+  TG  K  +L TG YA +R QF + +   E I+E LARI GN    D+A  L
Sbjct:  305 EEELGITCALIPTSTPGVEIGRRHFPLNVPFQNGPTRGNDIFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALATGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASL 433

HSP 2 Score: 56.6102 bits (135), Expect = 5.697e-48
Identity = 26/46 (56.52%), Postives = 32/46 (69.57%), Query Frame = -3 
Query:  394 GNGKARETLGWRLNWEKRYITLGPVATLLGLASKAHDPVHLLGDKE 531
            G  + ++ LG RL W KRYITL P+AT+LGLA K  DP  LLG +E
Sbjct:  261 GEWQGQQVLGMRLTWNKRYITLAPIATVLGLAFKLSDPDRLLGGEE 306

HSP 3 Score: 25.409 bits (54), Expect = 5.697e-48
Identity = 11/19 (57.89%), Postives = 15/19 (78.95%), Query Frame = -2 
Query:  515 AGSIVLTPGVICKGEWEGK 571
            AG+I  T GV+C GEW+G+
Sbjct:  249 AGAIPDT-GVVCMGEWQGQ 266
[Close]
BLAST of EY679511 vs. ExPASy Swiss-Prot
Match: FADE_SALTI (Acyl-coenzyme A dehydrogenase OS=Salmonella typhi GN=fadE PE=3 SV=1)

HSP 1 Score: 152.14 bits (383), Expect = 5.697e-48
Identity = 69/129 (53.49%), Postives = 90/129 (69.77%), Query Frame = -1 
Query:   12 KKDLGISLALIPTDTPGVEIGRRHLPLGAAFMNGPNSGKDVFIPLEFLIGGQEMLGKGWMMLMNCLSVGRSISLPAVGTGAAKFTSLVTGQYAQVREQFNVPLAAFESIQEALARIGGNVKRRDSARML 398
            +++LGI+ ALIPT TPGVEIGRRH PL   F NGP  G D+F+P++++IGG +M G+GW ML+ CLSVGR I+LP+  TG  K  +L TG YA +R QF + +   E I+E LARI GN    D+A  L
Sbjct:  305 EEELGITCALIPTSTPGVEIGRRHFPLNVPFQNGPTRGNDIFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALATGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASL 433

HSP 2 Score: 56.6102 bits (135), Expect = 5.697e-48
Identity = 26/46 (56.52%), Postives = 32/46 (69.57%), Query Frame = -3 
Query:  394 GNGKARETLGWRLNWEKRYITLGPVATLLGLASKAHDPVHLLGDKE 531
            G  + ++ LG RL W KRYITL P+AT+LGLA K  DP  LLG +E
Sbjct:  261 GEWQGQQVLGMRLTWNKRYITLAPIATVLGLAFKLSDPDRLLGGEE 306

HSP 3 Score: 25.409 bits (54), Expect = 5.697e-48
Identity = 11/19 (57.89%), Postives = 15/19 (78.95%), Query Frame = -2 
Query:  515 AGSIVLTPGVICKGEWEGK 571
            AG+I  T GV+C GEW+G+
Sbjct:  249 AGAIPDT-GVVCMGEWQGQ 266
[Close]
BLAST of EY679511 vs. ExPASy Swiss-Prot
Match: FADE_YERPE (Acyl-coenzyme A dehydrogenase OS=Yersinia pestis GN=fadE PE=3 SV=1)

HSP 1 Score: 145.976 bits (367), Expect = 2.986e-34
Identity = 67/127 (52.76%), Postives = 87/127 (68.50%), Query Frame = -1 
Query:   12 DLGISLALIPTDTPGVEIGRRHLPLGAAFMNGPNSGKDVFIPLEFLIGGQEMLGKGWMMLMNCLSVGRSISLPAVGTGAAKFTSLVTGQYAQVREQFNVPLAAFESIQEALARIGGNVKRRDSARML 392
            DLGI+ ALIPT TPGVEIG RH PL   F NGP  G D+F+P++++IGG +M G+GW ML+ CLSVGR I+LP+  TG+ K  ++  G YA +R QF + +   E I+E LARI GN    D+A  L
Sbjct:  307 DLGITCALIPTHTPGVEIGHRHFPLNIPFQNGPTRGNDIFVPIDYIIGGPQMAGQGWRMLVECLSVGRGITLPSNATGSLKSVAMGIGAYAYIRRQFKISIGKMEGIEEPLARIAGNTYVMDAAATL 433
[Close]
BLAST of EY679511 vs. ExPASy Swiss-Prot
Match: HEMX_PROMI (Putative uroporphyrinogen-III C-methyltransferase (Fragment) OS=Proteus mirabilis GN=hemX PE=3 SV=1)

HSP 1 Score: 116.701 bits (291), Expect = 1.383e-26
Identity = 52/86 (60.47%), Postives = 64/86 (74.42%), Query Frame = -1 
Query:  129 GISLALIPTDTPGVEIGRRHLPLGAAFMNGPNSGKDVFIPLEFLIGGQEMLGKGWMMLMNCLSVGRSISLPAVGTGAAKFTSLVTG 386
            GI+ ALIPTD  GVEIG RH PL   FMNGP  GKDVF+P++F+IGG +M G+GW ML+ CLSVGR I+LP+  TG  K  ++ TG
Sbjct:   80 GITCALIPTDVKGVEIGHRHFPLNVPFMNGPTRGKDVFVPIDFIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGLKSAAMATG 165

HSP 2 Score: 25.0238 bits (53), Expect = 1.383e-26
Identity = 10/17 (58.82%), Postives = 11/17 (64.71%), Query Frame = -3 
Query:  388 ASKAHDPVHLLGDKERP 438
            A+K  DP HLL D E P
Sbjct:   63 ATKLRDPDHLLSDNENP 79
The following BLAST results are available for this feature:
BLAST of EY679511 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 6
Match NameE-valueIdentityDescription
FADE_ECOLI1.529e-4856.25Acyl-coenzyme A dehydrogenase OS=Escherichia coli ... [more]
FADE_ECO571.989e-4856.25Acyl-coenzyme A dehydrogenase OS=Escherichia coli ... [more]
FADE_SALTY5.697e-4853.49Acyl-coenzyme A dehydrogenase OS=Salmonella typhim... [more]
FADE_SALTI5.697e-4853.49Acyl-coenzyme A dehydrogenase OS=Salmonella typhi ... [more]
FADE_YERPE2.986e-3452.76Acyl-coenzyme A dehydrogenase OS=Yersinia pestis G... [more]
HEMX_PROMI1.383e-2660.47Putative uroporphyrinogen-III C-methyltransferase ... [more]
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Properties
Property NameValue
Genbank descriptionCS00-C1-401-052-A05-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY679511 ID=EY679511; Name=EY679511; organism=Citrus sinensis; type=EST; length=938bp
GCATTGGCGGTGAGCATTCGCGCGCTGTCCCTCCTTTTTACGTTACCGCC
GATGCGGGCCAAGGCTTCCTGGATGCTTTCAAAGGCCGCCAGTGGTACGT
TGAACTGCTCACGCACCTGGGCATATTGGCCGGTCACCAGGCTGGTGAAC
TTGGCCGCGCCGGTGCCAACCGCTGGCAGGGAAATCGAACGGCCTACCGA
CAGGCAGTTCATCAGCATCATCCAGCCCTTGCCGAGCATTTCCTGGCCAC
CGATCAGGAACTCCAGGGGAATGAACACGTCCTTGCCGGAGTTGGGGCCG
TTCATGAACGCGGCACCCAGTGGCAGGTGACGGCGGCCGATTTCAACGCC
CGGTGTGTCGGTGGGGATCAGCGCGAGGCTGATGCCGAGGTCTTTCTTTG
TCGCCCAGCAAGTGGACCGGGTCGTGGGCCTTTGAGGCCAAGCCAAGCAG
GGTCGCCACCGGGCCGAGGGTGATGTAGCGTTTTTCCCAGTTCAGGCGCC
AGCCGAGGGTTTCCCTTGCCTTCCCATTCCCCTTTGCAGATCACCCCGGG
TGTCAGGACAATGGAGCCAGCAATCTGAGACCGCCAGGAGGACCCGGTCC
AGGCCAAAACACCGGATATATCTTCCCCCTCCGTCCAAGGAGGGGAAAAG
TAATGGGTTGCCATTGTTCCCTCCGGTGCCTGTAATATAACCAACATATT
ACCGCCAGTGGCCATAGGGATTTAGGGGAAACCTTGAACATGAGAAAAAC
AAAAGGCCGCCCCGCGGCCGGGATTGGCCAGCTTTTCATTCGCAACCGGA
GGGAATGGGGGCGTAAGGGGCGTAAAATACTTTTTCTGCCCATCAATATT
TCCTTTCTGGGAAATTTAATGATGGAACAAGTCAAAAACAATTGTTTGTT
TTTAATATGATACTCCCCCCTAAGGTGGTGGCGGGGGT
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