EY679477 | Citrus Genome Database

Overview


Name	EY679477
Unique Name	EY679477
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	894

Libraries


Library Name	Type
Sweet orange leaf, infected with CiLV	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY679477 vs. ExPASy Swiss-Prot
Match: MAOX_VITVI (NADP-dependent malic enzyme OS=Vitis vinifera PE=2 SV=1)

HSP 1 Score: 176.022 bits (445), Expect = 2.429e-45
Identity = 90/107 (84.11%), Postives = 94/107 (87.85%), Query Frame = 2

Query:    2 EETRKKICLVDSKGLIVSSRKDSLQHFKKPWAHEHEPVNNLLDAVKVIKPTILIGSSGVGRTFTKEVIEAMASFNEKPLILALSNPTSQSKCTAEEAYNLE*GSCNF 322
            EETRKKI LVDSKGLIV SRKDSLQ FKKPWAHEHEPV +LLDAVKVIKPT+LIGSSGVG+ FTKEVIEAMAS NEKPLILALSNPTSQS+CTAEEAY    G   F
Sbjct:  363 EETRKKIWLVDSKGLIVGSRKDSLQQFKKPWAHEHEPVKDLLDAVKVIKPTVLIGSSGVGKAFTKEVIEAMASCNEKPLILALSNPTSQSECTAEEAYTWTQGRAIF 469

HSP 2 Score: 28.4906 bits (62), Expect = 2.429e-45
Identity = 11/14 (78.57%), Postives = 11/14 (78.57%), Query Frame = 1

Query:  337 PFDPFEYNG*VFVP 378
            PFDP EYNG  FVP
Sbjct:  474 PFDPVEYNGKTFVP 487

[Close]

BLAST of EY679477 vs. ExPASy Swiss-Prot
Match: MAOX_POPTR (NADP-dependent malic enzyme OS=Populus trichocarpa PE=2 SV=3)

HSP 1 Score: 173.326 bits (438), Expect = 2.633e-44
Identity = 86/107 (80.37%), Postives = 93/107 (86.92%), Query Frame = 2

Query:    2 EETRKKICLVDSKGLIVSSRKDSLQHFKKPWAHEHEPVNNLLDAVKVIKPTILIGSSGVGRTFTKEVIEAMASFNEKPLILALSNPTSQSKCTAEEAYNLE*GSCNF 322
            EETRKKI L DSKGLIVSSRK+SLQHFKKPWAHEHEPV  LL+ VK IKP +LIG+SGVG+TFTKEVIEAMASFNEKPLILALSNPTSQS+CTA+EAY    G   F
Sbjct:  363 EETRKKIWLTDSKGLIVSSRKESLQHFKKPWAHEHEPVKGLLEVVKAIKPIVLIGTSGVGKTFTKEVIEAMASFNEKPLILALSNPTSQSECTAQEAYTWTKGKAIF 469

HSP 2 Score: 27.7202 bits (60), Expect = 2.633e-44
Identity = 11/14 (78.57%), Postives = 11/14 (78.57%), Query Frame = 1

Query:  337 PFDPFEYNG*VFVP 378
            PFDP EY G VFVP
Sbjct:  474 PFDPVEYEGKVFVP 487

[Close]

BLAST of EY679477 vs. ExPASy Swiss-Prot
Match: MAOC_ORYSJ (NADP-dependent malic enzyme, chloroplastic OS=Oryza sativa subsp. japonica GN=ME6 PE=2 SV=2)

HSP 1 Score: 165.622 bits (418), Expect = 6.840e-44
Identity = 81/107 (75.70%), Postives = 93/107 (86.92%), Query Frame = 2

Query:    2 EETRKKICLVDSKGLIVSSRKDSLQHFKKPWAHEHEPVNNLLDAVKVIKPTILIGSSGVGRTFTKEVIEAMASFNEKPLILALSNPTSQSKCTAEEAYNLE*GSCNF 322
            EE RKK+ L+DSKGLIV+SRK+SLQ FKKPWAHEHEPV  LLDAV+ IKPT+LIG+SGVG+TFTKEVIEAMASFNE+P+I +LSNPTS S+CTAEEAYN   G   F
Sbjct:  411 EECRKKVWLLDSKGLIVNSRKESLQAFKKPWAHEHEPVTTLLDAVQSIKPTVLIGTSGVGKTFTKEVIEAMASFNERPVIFSLSNPTSHSECTAEEAYNWSQGRAVF 517

HSP 2 Score: 27.7202 bits (60), Expect = 6.840e-44
Identity = 10/14 (71.43%), Postives = 13/14 (92.86%), Query Frame = 3

Query:  300 WSKGRAIFASGNTF 341
            WS+GRA+FASG+ F
Sbjct:  510 WSQGRAVFASGSPF 523

HSP 3 Score: 26.9498 bits (58), Expect = 6.840e-44
Identity = 10/14 (71.43%), Postives = 11/14 (78.57%), Query Frame = 1

Query:  337 PFDPFEYNG*VFVP 378
            PFDP EYNG + VP
Sbjct:  522 PFDPVEYNGKIHVP 535

[Close]

BLAST of EY679477 vs. ExPASy Swiss-Prot
Match: MAOX_PHAVU (NADP-dependent malic enzyme OS=Phaseolus vulgaris GN=ME1 PE=2 SV=1)

HSP 1 Score: 169.474 bits (428), Expect = 8.227e-43
Identity = 85/107 (79.44%), Postives = 91/107 (85.05%), Query Frame = 2

Query:    2 EETRKKICLVDSKGLIVSSRKDSLQHFKKPWAHEHEPVNNLLDAVKVIKPTILIGSSGVGRTFTKEVIEAMASFNEKPLILALSNPTSQSKCTAEEAYNLE*GSCNF 322
            EETRKKI LVDSKGLIVSSR +SLQ FKKPWAHEHEPV  LL+AVK IKPT+LIGSSG G+TFTKEV+E MAS NEKPLILALSNPTSQS+CTAEEAY    G   F
Sbjct:  361 EETRKKIWLVDSKGLIVSSRLESLQQFKKPWAHEHEPVKGLLEAVKAIKPTVLIGSSGAGKTFTKEVVETMASLNEKPLILALSNPTSQSECTAEEAYTWSKGRAIF 467

HSP 2 Score: 26.5646 bits (57), Expect = 8.227e-43
Identity = 10/14 (71.43%), Postives = 11/14 (78.57%), Query Frame = 1

Query:  337 PFDPFEYNG*VFVP 378
            PFDP EY G +FVP
Sbjct:  472 PFDPVEYEGKLFVP 485

[Close]

BLAST of EY679477 vs. ExPASy Swiss-Prot
Match: MAOC_FLAPR (NADP-dependent malic enzyme, chloroplastic OS=Flaveria pringlei GN=MODA PE=2 SV=1)

HSP 1 Score: 173.326 bits (438), Expect = 1.628e-42
Identity = 85/107 (79.44%), Postives = 93/107 (86.92%), Query Frame = 2

Query:    2 EETRKKICLVDSKGLIVSSRKDSLQHFKKPWAHEHEPVNNLLDAVKVIKPTILIGSSGVGRTFTKEVIEAMASFNEKPLILALSNPTSQSKCTAEEAYNLE*GSCNF 322
            EETRKKI LVDSKGLIV SR DSLQHFKKPWAH+HEPVN  LDAVK IKPT+LIGSSG G+TFTKEV+EAM+SFNEKP+ILALSNPTSQS+CTAE+AY    G   F
Sbjct:  419 EETRKKIWLVDSKGLIVRSRLDSLQHFKKPWAHDHEPVNKFLDAVKAIKPTVLIGSSGAGQTFTKEVVEAMSSFNEKPIILALSNPTSQSECTAEQAYTWSEGRTIF 525

[Close]

BLAST of EY679477 vs. ExPASy Swiss-Prot
Match: MAOC_SOLLC (NADP-dependent malic enzyme, chloroplastic (Fragment) OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 164.851 bits (416), Expect = 6.839e-42
Identity = 79/107 (73.83%), Postives = 92/107 (85.98%), Query Frame = 2

Query:    2 EETRKKICLVDSKGLIVSSRKDSLQHFKKPWAHEHEPVNNLLDAVKVIKPTILIGSSGVGRTFTKEVIEAMASFNEKPLILALSNPTSQSKCTAEEAYNLE*GSCNF 322
            EETRKKI +VDSKGLIV SR + LQHFK+PWAH+HEPV  L++AVK IKPT+LIGSSG GRTFTKEV++AMA+FNEKP+I ALSNPTSQS+CTAEEAY+   G   F
Sbjct:  346 EETRKKIWMVDSKGLIVKSRMEMLQHFKRPWAHDHEPVQELVNAVKSIKPTVLIGSSGAGRTFTKEVVQAMATFNEKPIIFALSNPTSQSECTAEEAYSWSEGRAIF 452

HSP 2 Score: 28.1054 bits (61), Expect = 6.839e-42
Identity = 11/14 (78.57%), Postives = 13/14 (92.86%), Query Frame = 3

Query:  300 WSKGRAIFASGNTF 341
            WS+GRAIFASG+ F
Sbjct:  445 WSEGRAIFASGSPF 458

[Close]

BLAST of EY679477 vs. ExPASy Swiss-Prot
Match: MAOC_FLATR (NADP-dependent malic enzyme, chloroplastic OS=Flaveria trinervia GN=MOD1 PE=2 SV=1)

HSP 1 Score: 166.392 bits (420), Expect = 1.255e-40
Identity = 79/107 (73.83%), Postives = 91/107 (85.05%), Query Frame = 2

Query:    2 EETRKKICLVDSKGLIVSSRKDSLQHFKKPWAHEHEPVNNLLDAVKVIKPTILIGSSGVGRTFTKEVIEAMASFNEKPLILALSNPTSQSKCTAEEAYNLE*GSCNF 322
            EE+RKK+ LVDSKGLIV SR DSLQHFKKPWAH+HEPVN  LDA+K I+PT+LIGSSG G+TFTKEV+E M+S NEKP+ILALSNPTSQS+CTAE+AY    G   F
Sbjct:  420 EESRKKVWLVDSKGLIVRSRLDSLQHFKKPWAHDHEPVNEFLDAIKTIRPTVLIGSSGTGQTFTKEVVETMSSLNEKPIILALSNPTSQSECTAEQAYTWSEGRAIF 526

HSP 2 Score: 22.3274 bits (46), Expect = 1.255e-40
Identity = 8/13 (61.54%), Postives = 10/13 (76.92%), Query Frame = 1

Query:  337 PFDPFEYNG*VFV 375
            PF P EYNG ++V
Sbjct:  531 PFKPVEYNGKLYV 543

[Close]

BLAST of EY679477 vs. ExPASy Swiss-Prot
Match: MAOX_MESCR (NADP-dependent malic enzyme OS=Mesembryanthemum crystallinum GN=MOD1 PE=2 SV=1)

HSP 1 Score: 157.147 bits (396), Expect = 5.103e-39
Identity = 76/107 (71.03%), Postives = 89/107 (83.18%), Query Frame = 2

Query:    2 EETRKKICLVDSKGLIVSSRKDSLQHFKKPWAHEHEPVNNLLDAVKVIKPTILIGSSGVGRTFTKEVIEAMASFNEKPLILALSNPTSQSKCTAEEAYNLE*GSCNF 322
            E+ RKKI LVDSKGL+VSSRK++LQ FK PWAHEHEP+  L+DAV+ IKPT+LIG+SG G+ FTKEV+EAMA+ N KPLILALSNPTSQS+CTAEEAY    G   F
Sbjct:  357 EQMRKKIWLVDSKGLVVSSRKETLQQFKLPWAHEHEPITTLIDAVQAIKPTVLIGTSGKGKQFTKEVVEAMANINAKPLILALSNPTSQSECTAEEAYTWSQGHAIF 463

HSP 2 Score: 26.1794 bits (56), Expect = 5.103e-39
Identity = 10/14 (71.43%), Postives = 10/14 (71.43%), Query Frame = 1

Query:  337 PFDPFEYNG*VFVP 378
            PFDP EY G  FVP
Sbjct:  468 PFDPVEYEGRTFVP 481

[Close]

BLAST of EY679477 vs. ExPASy Swiss-Prot
Match: MAOC_MAIZE (NADP-dependent malic enzyme, chloroplastic OS=Zea mays GN=MOD1 PE=1 SV=1)

HSP 1 Score: 158.303 bits (399), Expect = 1.913e-38
Identity = 77/107 (71.96%), Postives = 89/107 (83.18%), Query Frame = 2

Query:    2 EETRKKICLVDSKGLIVSSRKDSLQHFKKPWAHEHEPVNNLLDAVKVIKPTILIGSSGVGRTFTKEVIEAMASFNEKPLILALSNPTSQSKCTAEEAYNLE*GSCNF 322
            EE RKK+ LVDSKGLIV SRK SLQ FKKPWAHEHEP+  L DAV+ IKPT+LIG+SGVGRTFTKE+IEAM+SFNE+P+I +LSNPTS S+CTAE+AY    G   F
Sbjct:  408 EECRKKVWLVDSKGLIVDSRKGSLQPFKKPWAHEHEPLKTLYDAVQSIKPTVLIGTSGVGRTFTKEIIEAMSSFNERPIIFSLSNPTSHSECTAEQAYTWSQGRSIF 514

HSP 2 Score: 23.0978 bits (48), Expect = 1.913e-38
Identity = 9/14 (64.29%), Postives = 9/14 (64.29%), Query Frame = 1

Query:  337 PFDPFEYNG*VFVP 378
            PF P EY G  FVP
Sbjct:  519 PFAPVEYEGKTFVP 532

[Close]

BLAST of EY679477 vs. ExPASy Swiss-Prot
Match: MAOX_RAT (NADP-dependent malic enzyme OS=Rattus norvegicus GN=Me1 PE=1 SV=2)

HSP 1 Score: 108.612 bits (270), Expect = 5.353e-24
Identity = 56/107 (52.34%), Postives = 77/107 (71.96%), Query Frame = 2

Query:    2 EETRKKICLVDSKGLIVSSRKDSLQHFKKPWAHEHEPVNNLLDAVKVIKPTILIGSSGVGRTFTKEVIEAMASFNEKPLILALSNPTSQSKCTAEEAYNLE*GSCNF 322
            E+ R+KI LVDSKGLIV  R  SL   K+ +AHEHE + NL   V+ IKPT LIG + +G  FT+++++ MA+FNE+P+I ALSNPTS+++C+AEE Y +  G   F
Sbjct:  325 EKARQKIWLVDSKGLIVKGRA-SLTEEKEVFAHEHEEMKNLEAIVQKIKPTALIGVAAIGGAFTEQILKDMAAFNERPIIFALSNPTSKAECSAEECYKVTKGRAIF 430

HSP 2 Score: 24.2534 bits (51), Expect = 5.353e-24
Identity = 10/13 (76.92%), Postives = 12/13 (92.31%), Query Frame = 3

Query:  303 SKGRAIFASGNTF 341
            +KGRAIFASG+ F
Sbjct:  424 TKGRAIFASGSPF 436

The following BLAST results are available for this feature:

BLAST of EY679477 vs. ExPASy Swiss-Prot

BLAST of EY679477 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 29


Match Name	E-value	Identity	Description
MAOX_VITVI	2.429e-45	84.11	NADP-dependent malic enzyme OS=Vitis vinifera PE=2...	[more]
MAOX_POPTR	2.633e-44	80.37	NADP-dependent malic enzyme OS=Populus trichocarpa...	[more]
MAOC_ORYSJ	6.840e-44	75.70	NADP-dependent malic enzyme, chloroplastic OS=Oryz...	[more]
MAOX_PHAVU	8.227e-43	79.44	NADP-dependent malic enzyme OS=Phaseolus vulgaris ...	[more]
MAOC_FLAPR	1.628e-42	79.44	NADP-dependent malic enzyme, chloroplastic OS=Flav...	[more]
MAOC_SOLLC	6.839e-42	73.83	NADP-dependent malic enzyme, chloroplastic (Fragme...	[more]
MAOC_FLATR	1.255e-40	73.83	NADP-dependent malic enzyme, chloroplastic OS=Flav...	[more]
MAOX_MESCR	5.103e-39	71.03	NADP-dependent malic enzyme OS=Mesembryanthemum cr...	[more]
MAOC_MAIZE	1.913e-38	71.96	NADP-dependent malic enzyme, chloroplastic OS=Zea ...	[more]
MAOX_RAT	5.353e-24	52.34	NADP-dependent malic enzyme OS=Rattus norvegicus G...	[more]

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Properties


Property Name	Value
Genbank description	CS00-C1-401-057-F04-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY679477 ID=EY679477; Name=EY679477; organism=Citrus sinensis; type=EST; length=894bp
GGAAGAGACCCGGAAGAAGATATGTCTTGTAGACTCAAAGGGACTGATAG
TCAGCTCCCGGAAAGATTCACTTCAACACTTCAAGAAGCCTTGGGCTCAT
GAACATGAGCCTGTCAACAATCTCTTAGATGCTGTCAAGGTAATCAAGCC
AACAATTTTGATTGGATCATCTGGAGTTGGAAGAACTTTTACAAAAGAAG
TAATCGAGGCCATGGCCTCCTTCAATGAGAAACCTCTCATCCTGGCTCTC
TCCAACCCAACCTCACAGTCAAAATGTACTGCTGAAGAAGCTTACAATTT
GGAGTAAGGGTCGTGCAATTTTCGCTAGTGGAAATACCTTTTGATCCTTT
TGAATACAATGGATAAGTTTTTGTTCCTAGGCTATGACAAACATGATAAC
ATCTTCCACTGGATATGGTGGGGGGATGGTCACCACTGGAACATATAGGT
AACTGACAAATTGTTTAGGATACCATGTAATACCTGATAAATCTATATAT
AAAAATATATCAAAAAAGTATATTATACTCTTCATTTCCATTATATAATA
TTTTCACCAAAAAATGGCAAATTTCCTCTGAATTACATAACTATATAGGT
ATAGAACAGTCAATAAAATTTAAACTTGTGAAAGAGAGTAATTAAATAAA
AGAGAATTTTATATGGTACATATAATATAAAAAAAAGAAAATCATGGTGT
CAGATTACATATTAAACAAATATACTTATTAAAATTAATTTAGAACAACA
TTTTTTCATATATTAAGAACCGTCATATTGATAAATGAATAATACATCAA
ATAGGTAAGTCTTCGTTAACATCTAAAGATAATGTTAGTAAACGATATGT
AGAATATAGAAATAAAACATATAAATATACAGATGGTAATAATA