EY679227

Overview
NameEY679227
Unique NameEY679227
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length1065
Libraries
Library NameType
Sweet orange leaf, infected with CiLVcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY679227 vs. ExPASy Swiss-Prot
Match: UBC35_ARATH (Ubiquitin carrier protein E2 35 OS=Arabidopsis thaliana GN=UBC35 PE=1 SV=1)

HSP 1 Score: 140.969 bits (354), Expect = 1.214e-45
Identity = 66/73 (90.41%), Postives = 72/73 (98.63%), Query Frame = 1 
Query:  130 MASGNLPRRIVKETQRLLSEPAPGISASPAEDNMRYFNVMILGPSQSPYEGGVFKLELFLPEDYPMSAPKVRF 348
            MA+ NLPRRI+KETQRLLSEPAPGISASP+EDNMRYFNVMILGP+QSPYEGGVFKLELFLPE+YPM+APKVRF
Sbjct:    1 MANSNLPRRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRF 73

HSP 2 Score: 64.6994 bits (156), Expect = 1.214e-45
Identity = 45/81 (55.56%), Postives = 48/81 (59.26%), Query Frame = 2 
Query:  347 FLTKIYHPNIDKLGRICLDILKV*MESRPAKTAQFLLSIQALLECS*PIRSPFSELTYAMH*NDNETEAC*YCEMEWTLVY 589
            FLTKIYHPNIDKLGRICLDILK    S   +    LLSIQALL    P   P SE   A H   NE EA    + EWT +Y
Sbjct:   73 FLTKIYHPNIDKLGRICLDILKD-KWSPALQIRTVLLSIQALLSAPNP-DDPLSE-NIAKHWKSNEAEAVDTAK-EWTRLY 149
[Close]
BLAST of EY679227 vs. ExPASy Swiss-Prot
Match: UBC36_ARATH (Ubiquitin carrier protein E2 36 OS=Arabidopsis thaliana GN=UBC36 PE=1 SV=1)

HSP 1 Score: 139.428 bits (350), Expect = 3.502e-45
Identity = 65/73 (89.04%), Postives = 72/73 (98.63%), Query Frame = 1 
Query:  130 MASGNLPRRIVKETQRLLSEPAPGISASPAEDNMRYFNVMILGPSQSPYEGGVFKLELFLPEDYPMSAPKVRF 348
            MA+ NLPRRI+KETQRLLSEPAPGISASP+E+NMRYFNVMILGP+QSPYEGGVFKLELFLPE+YPM+APKVRF
Sbjct:    1 MANSNLPRRIIKETQRLLSEPAPGISASPSEENMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRF 73

HSP 2 Score: 64.6994 bits (156), Expect = 3.502e-45
Identity = 45/81 (55.56%), Postives = 47/81 (58.02%), Query Frame = 2 
Query:  347 FLTKIYHPNIDKLGRICLDILKV*MESRPAKTAQFLLSIQALLECS*PIRSPFSELTYAMH*NDNETEAC*YCEMEWTLVY 589
            FLTKIYHPNIDKLGRICLDILK    S   +    LLSIQALL    P   P SE   A H   NE EA      EWT +Y
Sbjct:   73 FLTKIYHPNIDKLGRICLDILKD-KWSPALQIRTVLLSIQALLSAPNP-DDPLSE-NIAKHWKSNEAEAV-ETAKEWTRLY 149
[Close]
BLAST of EY679227 vs. ExPASy Swiss-Prot
Match: UBE2N_DICDI (Probable ubiquitin-conjugating enzyme E2 N OS=Dictyostelium discoideum GN=ube2n PE=3 SV=2)

HSP 1 Score: 112.464 bits (280), Expect = 1.212e-35
Identity = 52/68 (76.47%), Postives = 59/68 (86.76%), Query Frame = 1 
Query:  145 LPRRIVKETQRLLSEPAPGISASPAEDNMRYFNVMILGPSQSPYEGGVFKLELFLPEDYPMSAPKVRF 348
            LP+RI+KETQRL++EPAPGISA+PA DN RYF V I GP  SP+EGGVF LELFL +DYPMSAPKVRF
Sbjct:    4 LPKRIIKETQRLMTEPAPGISATPATDNFRYFKVAISGPLDSPFEGGVFNLELFLTDDYPMSAPKVRF 71

HSP 2 Score: 59.6918 bits (143), Expect = 1.212e-35
Identity = 43/81 (53.09%), Postives = 46/81 (56.79%), Query Frame = 2 
Query:  347 FLTKIYHPNIDKLGRICLDILKV*MESRPAKTAQFLLSIQALLECS*PIRSPFSELTYAMH*NDNETEAC*YCEMEWTLVY 589
            FLTKIYHPNIDKLGRICLDILK    S   +    LLSIQALL    P   P +    A H   NE EA      EWT +Y
Sbjct:   71 FLTKIYHPNIDKLGRICLDILKD-KWSPALQIRTVLLSIQALLSAPNP-DDPLAN-DVAEHWKTNEKEAMARAR-EWTRLY 147
[Close]
BLAST of EY679227 vs. ExPASy Swiss-Prot
Match: UBE2N_DROME (Ubiquitin-conjugating enzyme E2 N OS=Drosophila melanogaster GN=ben PE=2 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 5.929e-35
Identity = 52/69 (75.36%), Postives = 62/69 (89.86%), Query Frame = 1 
Query:  142 NLPRRIVKETQRLLSEPAPGISASPAEDNMRYFNVMILGPSQSPYEGGVFKLELFLPEDYPMSAPKVRF 348
            +LPRRI+KETQRL+ EP PGI+A P E+N RYF+V++ GP+ SP+EGGVFKLELFLPEDYPMSAPKVRF
Sbjct:    3 SLPRRIIKETQRLMQEPVPGINAIPDENNARYFHVIVTGPNDSPFEGGVFKLELFLPEDYPMSAPKVRF 71

HSP 2 Score: 53.9138 bits (128), Expect = 5.929e-35
Identity = 39/84 (46.43%), Postives = 46/84 (54.76%), Query Frame = 2 
Query:  347 FLTKIYHPNIDKLGRICLDILKV*MESRPAKTAQFLLSIQALLEC---S*PIRSPFSELTYAMH*NDNETEAC*YCEMEWTLVY 589
            F+TKIYHPNID+LGRICLD+LK    S   +    LLSIQALL       P+ +  +EL        NE EA      EWT  Y
Sbjct:   71 FITKIYHPNIDRLGRICLDVLKD-KWSPALQIRTILLSIQALLSAPNPDDPLANDVAELWKV-----NEAEAIRNAR-EWTQKY 147
[Close]
BLAST of EY679227 vs. ExPASy Swiss-Prot
Match: UBE2N_RAT (Ubiquitin-conjugating enzyme E2 N OS=Rattus norvegicus GN=Ube2n PE=1 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 6.399e-34
Identity = 51/68 (75.00%), Postives = 58/68 (85.29%), Query Frame = 1 
Query:  145 LPRRIVKETQRLLSEPAPGISASPAEDNMRYFNVMILGPSQSPYEGGVFKLELFLPEDYPMSAPKVRF 348
            LPRRI+KETQRLL+EP PGI A P E N RYF+V+I GP  SP+EGG FKLELFLPE+YPM+APKVRF
Sbjct:    4 LPRRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPKVRF 71

HSP 2 Score: 53.9138 bits (128), Expect = 6.399e-34
Identity = 30/48 (62.50%), Postives = 33/48 (68.75%), Query Frame = 2 
Query:  347 FLTKIYHPNIDKLGRICLDILKV*MESRPAKTAQFLLSIQALLECS*P 490
            F+TKIYHPN+DKLGRICLDILK    S   +    LLSIQALL    P
Sbjct:   71 FMTKIYHPNVDKLGRICLDILKD-KWSPALQIRTVLLSIQALLSAPNP 117
[Close]
BLAST of EY679227 vs. ExPASy Swiss-Prot
Match: UBE2N_PONAB (Ubiquitin-conjugating enzyme E2 N OS=Pongo abelii GN=UBE2N PE=2 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 6.399e-34
Identity = 51/68 (75.00%), Postives = 58/68 (85.29%), Query Frame = 1 
Query:  145 LPRRIVKETQRLLSEPAPGISASPAEDNMRYFNVMILGPSQSPYEGGVFKLELFLPEDYPMSAPKVRF 348
            LPRRI+KETQRLL+EP PGI A P E N RYF+V+I GP  SP+EGG FKLELFLPE+YPM+APKVRF
Sbjct:    4 LPRRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPKVRF 71

HSP 2 Score: 53.9138 bits (128), Expect = 6.399e-34
Identity = 30/48 (62.50%), Postives = 33/48 (68.75%), Query Frame = 2 
Query:  347 FLTKIYHPNIDKLGRICLDILKV*MESRPAKTAQFLLSIQALLECS*P 490
            F+TKIYHPN+DKLGRICLDILK    S   +    LLSIQALL    P
Sbjct:   71 FMTKIYHPNVDKLGRICLDILKD-KWSPALQIRTVLLSIQALLSAPNP 117
[Close]
BLAST of EY679227 vs. ExPASy Swiss-Prot
Match: UBE2N_MOUSE (Ubiquitin-conjugating enzyme E2 N OS=Mus musculus GN=Ube2n PE=1 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 6.399e-34
Identity = 51/68 (75.00%), Postives = 58/68 (85.29%), Query Frame = 1 
Query:  145 LPRRIVKETQRLLSEPAPGISASPAEDNMRYFNVMILGPSQSPYEGGVFKLELFLPEDYPMSAPKVRF 348
            LPRRI+KETQRLL+EP PGI A P E N RYF+V+I GP  SP+EGG FKLELFLPE+YPM+APKVRF
Sbjct:    4 LPRRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPKVRF 71

HSP 2 Score: 53.9138 bits (128), Expect = 6.399e-34
Identity = 30/48 (62.50%), Postives = 33/48 (68.75%), Query Frame = 2 
Query:  347 FLTKIYHPNIDKLGRICLDILKV*MESRPAKTAQFLLSIQALLECS*P 490
            F+TKIYHPN+DKLGRICLDILK    S   +    LLSIQALL    P
Sbjct:   71 FMTKIYHPNVDKLGRICLDILKD-KWSPALQIRTVLLSIQALLSAPNP 117
[Close]
BLAST of EY679227 vs. ExPASy Swiss-Prot
Match: UBE2N_HUMAN (Ubiquitin-conjugating enzyme E2 N OS=Homo sapiens GN=UBE2N PE=1 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 6.399e-34
Identity = 51/68 (75.00%), Postives = 58/68 (85.29%), Query Frame = 1 
Query:  145 LPRRIVKETQRLLSEPAPGISASPAEDNMRYFNVMILGPSQSPYEGGVFKLELFLPEDYPMSAPKVRF 348
            LPRRI+KETQRLL+EP PGI A P E N RYF+V+I GP  SP+EGG FKLELFLPE+YPM+APKVRF
Sbjct:    4 LPRRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPKVRF 71

HSP 2 Score: 53.9138 bits (128), Expect = 6.399e-34
Identity = 30/48 (62.50%), Postives = 33/48 (68.75%), Query Frame = 2 
Query:  347 FLTKIYHPNIDKLGRICLDILKV*MESRPAKTAQFLLSIQALLECS*P 490
            F+TKIYHPN+DKLGRICLDILK    S   +    LLSIQALL    P
Sbjct:   71 FMTKIYHPNVDKLGRICLDILKD-KWSPALQIRTVLLSIQALLSAPNP 117
[Close]
BLAST of EY679227 vs. ExPASy Swiss-Prot
Match: UBE2N_BOVIN (Ubiquitin-conjugating enzyme E2 N OS=Bos taurus GN=UBE2N PE=2 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 6.399e-34
Identity = 51/68 (75.00%), Postives = 58/68 (85.29%), Query Frame = 1 
Query:  145 LPRRIVKETQRLLSEPAPGISASPAEDNMRYFNVMILGPSQSPYEGGVFKLELFLPEDYPMSAPKVRF 348
            LPRRI+KETQRLL+EP PGI A P E N RYF+V+I GP  SP+EGG FKLELFLPE+YPM+APKVRF
Sbjct:    4 LPRRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPKVRF 71

HSP 2 Score: 53.9138 bits (128), Expect = 6.399e-34
Identity = 30/48 (62.50%), Postives = 33/48 (68.75%), Query Frame = 2 
Query:  347 FLTKIYHPNIDKLGRICLDILKV*MESRPAKTAQFLLSIQALLECS*P 490
            F+TKIYHPN+DKLGRICLDILK    S   +    LLSIQALL    P
Sbjct:   71 FMTKIYHPNVDKLGRICLDILKD-KWSPALQIRTVLLSIQALLSAPNP 117
[Close]
BLAST of EY679227 vs. ExPASy Swiss-Prot
Match: UBE2N_MACFA (Ubiquitin-conjugating enzyme E2 N OS=Macaca fascicularis GN=UBE2N PE=2 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 3.125e-33
Identity = 51/68 (75.00%), Postives = 58/68 (85.29%), Query Frame = 1 
Query:  145 LPRRIVKETQRLLSEPAPGISASPAEDNMRYFNVMILGPSQSPYEGGVFKLELFLPEDYPMSAPKVRF 348
            LPRRI+KETQRLL+EP PGI A P E N RYF+V+I GP  SP+EGG FKLELFLPE+YPM+APKVRF
Sbjct:    4 LPRRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPKVRF 71

HSP 2 Score: 51.6026 bits (122), Expect = 3.125e-33
Identity = 29/48 (60.42%), Postives = 32/48 (66.67%), Query Frame = 2 
Query:  347 FLTKIYHPNIDKLGRICLDILKV*MESRPAKTAQFLLSIQALLECS*P 490
            F+TKIYHPN+DK GRICLDILK    S   +    LLSIQALL    P
Sbjct:   71 FMTKIYHPNVDKSGRICLDILKD-KWSPALQIRTVLLSIQALLSAPNP 117
The following BLAST results are available for this feature:
BLAST of EY679227 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 112
Match NameE-valueIdentityDescription
UBC35_ARATH1.214e-4590.41Ubiquitin carrier protein E2 35 OS=Arabidopsis tha... [more]
UBC36_ARATH3.502e-4589.04Ubiquitin carrier protein E2 36 OS=Arabidopsis tha... [more]
UBE2N_DICDI1.212e-3576.47Probable ubiquitin-conjugating enzyme E2 N OS=Dict... [more]
UBE2N_DROME5.929e-3575.36Ubiquitin-conjugating enzyme E2 N OS=Drosophila me... [more]
UBE2N_RAT6.399e-3475.00Ubiquitin-conjugating enzyme E2 N OS=Rattus norveg... [more]
UBE2N_PONAB6.399e-3475.00Ubiquitin-conjugating enzyme E2 N OS=Pongo abelii ... [more]
UBE2N_MOUSE6.399e-3475.00Ubiquitin-conjugating enzyme E2 N OS=Mus musculus ... [more]
UBE2N_HUMAN6.399e-3475.00Ubiquitin-conjugating enzyme E2 N OS=Homo sapiens ... [more]
UBE2N_BOVIN6.399e-3475.00Ubiquitin-conjugating enzyme E2 N OS=Bos taurus GN... [more]
UBE2N_MACFA3.125e-3375.00Ubiquitin-conjugating enzyme E2 N OS=Macaca fascic... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-401-056-G08-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY679227 ID=EY679227; Name=EY679227; organism=Citrus sinensis; type=EST; length=1065bp
TTCGGTGGTCCCCGGGAATTTCCCGGGGTCCGACCCACCGCGTTCCGGAA
AAGAAAGAGGAAAAAGAGGGAGAGGGAAAAAAAGACGATCGATTTCTGAC
TCATCTCTGTAAAATCTCTTGGAATTCCGATGGCTAGTGGCAACCTCCCT
CGCAGAATTGTCAAGGAAACCCAGCGGCTGCTCAGTGAACCAGCTCCAGG
CATTAGTGCTTCTCCTGCCGAAGACAACATGCGATATTTTAATGTAATGA
TTCTTGGTCCATCTCAGTCTCCTTATGAAGGTGGGGTTTTCAAGTTGGAG
TTGTTTTTGCCTGAAGATTATCCAATGTCCGCTCCTAAGGTTCGATTTTC
TGACCAAAATATATCATCCTAACATAGACAAGCTTGGAAGGATATGCCTT
GATATTTTGAAAGTATAAATGGAGTCCCGTCCTGCAAAAACCGCACAATT
CCTGCTGAGTATCCAAGCTCTTTTAGAATGCTCCTAACCCATACGATCAC
CTTTTTCTGAATTAACATATGCAATGCATTGAAATGACAATGAGACTGAA
GCCTGCTGATACTGCGAAATGGAATGGACCCTGGTTTATTGTTAGTTGTT
GGGTTAAAGGCTAAATCTTTGTTTCAAAGAATATTCTCTAACTGATTAAT
GTTAAAAACCCCTATTTTGGTTCATAGAACCGAATGGGCCAAAAATGGTT
TTCCTCAACACGTCCGTGGTATTTAATGGTTCCAAGTTGGAATAACACTT
TATTTTCATTGGGTATACTTTTTTAATCATAAAAAAAAAAAAAAAAAATA
AAAAAATATGTGGAGGTCCTCTTTCCTTAGGTATTTATACTTTTTCCTTT
CTTTTGTTATCTGTATACTCCTTTTGTCCCTTTTTATTCTTGGTCCTCCA
TATTCTCAAATATTATATGTGGGACCTTCTTTTTTCTGATCTTAAATGCT
GGAACACGTGGAGTTATTAACCCATGTTGGGGGTTTGTACGCCCTACTTA
TAGATTTATGCTCGCGTGTTTGATGCTAGCATTAGCAACCCTCCCTCCTC
CTATGTTTCACACAC
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